BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 189 185 142.72 14.53 2 4 202 198 142.90 14.61 3 4 203 199 142.72 14.53 4 4 204 200 142.69 14.54 5 4 205 201 138.92 12.70 6 4 227 223 112.75 0.30 7 5 35 30 672.13 62.85 8 5 36 31 672.11 62.86 9 5 37 32 672.12 62.84 10 5 38 33 672.12 62.83 11 5 205 200 142.69 14.54 12 6 36 30 672.13 62.84 13 6 37 31 672.13 62.84 14 6 38 32 672.15 62.82 15 6 221 215 112.86 0.34 16 6 227 221 112.91 0.35 17 6 228 222 112.88 0.33 18 6 229 223 112.89 0.34 19 6 244 238 38.45 0.21 20 6 246 240 29.52 0.90 21 6 247 241 22.48 0.24 22 7 38 31 672.15 62.82 23 7 40 33 672.15 62.82 24 7 90 83 150.81 16.21 25 7 161 154 144.59 15.25 26 7 205 198 144.18 15.08 27 7 207 200 142.69 14.54 28 7 227 220 112.98 0.32 29 7 228 221 112.91 0.34 30 7 229 222 112.91 0.34 31 8 37 29 672.26 62.79 32 8 38 30 672.21 62.80 33 8 39 31 672.17 62.82 34 8 40 32 672.16 62.81 35 8 229 221 112.91 0.35 36 8 231 223 112.87 0.33 37 8 240 232 56.55 0.33 38 8 243 235 42.52 0.01 39 8 244 236 38.39 0.21 40 9 40 31 672.16 62.82 41 9 229 220 113.00 0.32 42 9 230 221 112.91 0.35 43 9 231 222 112.87 0.33 44 9 240 231 56.63 0.31 45 10 40 30 672.21 62.81 46 10 41 31 672.16 62.82 47 10 42 32 672.15 62.82 48 10 231 221 112.91 0.35 49 10 235 225 78.42 0.01 50 10 240 230 56.63 0.31 51 11 64 53 358.85 40.65 52 11 92 81 150.78 16.21 53 11 95 84 150.31 16.27 54 11 230 219 113.30 0.35 55 11 231 220 112.99 0.32 56 11 232 221 112.91 0.35 57 11 235 224 86.25 1.22 58 12 15 3 856.10 1.58 59 12 21 9 673.09 62.94 60 12 24 12 672.75 62.88 61 12 25 13 672.74 62.88 62 12 41 29 672.29 62.77 63 12 42 30 672.21 62.80 64 12 43 31 672.19 62.82 65 12 44 32 672.18 62.82 66 12 64 52 358.95 40.60 67 12 235 223 111.39 0.18 68 12 236 224 84.67 2.00 69 13 44 31 672.19 62.82 70 13 64 51 358.95 40.60 71 13 95 82 152.76 17.16 72 13 97 84 152.81 17.28 73 14 44 30 672.23 62.79 74 15 21 6 820.62 3.97 75 15 44 29 672.29 62.77 76 15 236 221 112.75 0.42 77 16 20 4 832.41 1.93 78 16 21 5 795.36 5.93 79 16 47 31 672.19 62.76 80 17 20 3 852.77 0.10 81 17 21 4 830.15 0.80 82 17 47 30 672.10 62.70 83 18 21 3 849.52 3.06 84 18 22 4 830.16 0.80 85 18 47 29 672.11 62.70 86 18 50 32 613.62 60.51 87 19 22 3 856.50 1.77 88 19 23 4 834.12 1.07 89 20 23 3 847.69 6.17 90 20 24 4 837.59 2.92 91 21 24 3 829.52 1.59 92 21 25 4 829.52 1.59 93 21 47 26 672.03 62.69 94 21 50 29 612.85 60.80 95 21 236 215 110.98 0.22 96 22 25 3 845.00 6.15 97 22 26 4 824.84 3.93 98 22 50 28 601.29 55.02 99 23 26 3 834.62 0.95 100 23 27 4 832.61 0.06 101 24 27 3 849.03 6.26 102 24 28 4 825.09 4.11 103 24 235 211 111.00 0.22 104 24 236 212 90.56 0.87 105 25 28 3 832.87 7.12 106 25 29 4 824.27 3.93 107 25 50 25 596.70 55.26 108 25 51 26 582.01 56.55 109 25 54 29 378.35 32.19 110 25 55 30 361.94 40.39 111 26 29 3 844.14 3.83 112 26 30 4 823.87 4.00 113 27 30 3 853.43 1.04 114 27 31 4 823.69 3.95 115 27 238 211 70.98 0.28 116 28 31 3 835.78 2.10 117 28 33 5 823.62 3.98 118 28 58 30 361.69 40.47 119 28 240 212 59.34 0.30 120 28 241 213 56.65 0.33 121 28 244 216 56.42 0.27 122 29 54 25 378.35 32.20 123 29 55 26 361.79 40.47 124 29 56 27 361.75 40.48 125 29 58 29 361.70 40.46 126 31 58 27 361.69 40.46 127 31 241 210 56.59 0.32 128 32 245 213 42.94 0.23 129 33 58 25 360.55 40.40 130 33 59 26 360.56 40.40 131 33 241 208 56.43 0.28 132 33 244 211 42.48 0.03 133 33 245 212 40.66 0.75 134 34 37 3 876.60 7.24 135 34 245 211 39.69 0.26 136 34 248 214 31.14 0.01 137 37 244 207 38.44 0.21 138 38 205 167 144.58 15.25 139 39 59 20 359.56 40.70 140 39 60 21 359.32 40.81 141 39 240 201 56.62 0.30 142 39 244 205 38.42 0.21 143 40 59 19 359.73 40.75 144 40 60 20 359.55 40.70 145 40 61 21 359.31 40.81 146 40 62 22 359.02 40.66 147 40 90 50 150.82 16.21 148 41 55 14 361.03 40.40 149 41 59 18 360.00 40.67 150 41 60 19 359.89 40.71 151 41 61 20 359.50 40.71 152 41 62 21 359.31 40.81 153 41 240 199 56.67 0.33 154 42 62 20 359.03 40.66 155 42 64 22 358.86 40.65 156 43 47 4 765.84 26.39 157 43 48 5 534.38 33.19 158 43 51 8 525.46 37.34 159 43 62 19 359.72 40.74 160 43 64 21 358.99 40.65 161 44 47 3 838.82 2.46 162 44 48 4 819.72 0.54 163 44 64 20 359.22 40.52 164 45 48 3 840.59 1.70 165 45 78 33 167.00 17.79 166 47 50 3 847.71 1.86 167 47 51 4 837.59 3.19 168 48 51 3 850.06 3.04 169 48 52 4 847.43 1.73 170 48 61 13 360.76 40.45 171 48 62 14 360.76 40.46 172 48 63 15 360.45 40.49 173 48 86 38 159.33 20.18 174 48 88 40 151.12 16.16 175 49 52 3 850.31 0.30 176 49 53 4 839.90 2.01 177 49 86 37 157.83 20.90 178 50 53 3 851.92 0.52 179 50 54 4 838.64 2.64 180 51 54 3 850.64 0.55 181 51 55 4 838.63 2.64 182 51 61 10 360.92 40.41 183 52 55 3 851.26 0.86 184 52 56 4 851.26 0.86 185 52 57 5 837.70 3.11 186 52 59 7 618.22 34.09 187 52 60 8 436.86 78.22 188 52 61 9 360.93 40.41 189 52 86 34 150.06 15.99 190 52 87 35 148.94 15.89 191 55 59 4 806.64 3.73 192 55 61 6 361.62 40.57 193 56 59 3 845.20 1.63 194 61 88 27 150.88 16.18 195 61 89 28 150.92 16.16 196 61 90 29 150.83 16.21 197 62 90 28 150.83 16.21 198 62 91 29 150.90 16.20 199 62 92 30 150.83 16.21 200 63 83 20 159.40 20.19 201 63 90 27 151.08 16.18 202 63 91 28 151.07 16.18 203 63 92 29 151.06 16.18 204 64 92 28 151.08 16.18 205 65 91 26 153.13 17.11 206 65 92 27 153.10 17.12 207 65 93 28 153.09 17.13 208 65 98 33 153.47 17.27 209 65 112 47 163.58 18.95 210 66 78 12 167.09 17.82 211 66 80 14 165.03 18.78 212 66 83 17 165.07 18.75 213 66 91 25 159.34 20.16 214 66 93 27 159.31 20.16 215 66 112 46 163.86 19.05 216 66 116 50 164.56 18.86 217 67 74 7 168.68 18.03 218 67 77 10 168.30 18.09 219 67 78 11 168.27 18.10 220 67 80 13 167.21 17.88 221 67 112 45 164.62 18.91 222 67 115 48 165.69 18.64 223 68 108 40 164.70 18.93 224 68 111 43 164.78 18.96 225 68 112 44 164.67 18.93 226 68 115 47 164.95 19.01 227 69 74 5 823.09 9.63 228 69 79 10 168.37 18.09 229 69 80 11 168.35 18.09 230 69 115 46 167.32 18.48 231 70 79 9 168.52 18.02 232 70 115 45 168.53 18.03 233 70 119 49 168.40 18.11 234 74 77 3 853.99 0.43 235 78 82 4 814.88 1.29 236 78 83 5 824.26 8.78 237 79 82 3 828.04 7.88 238 79 83 4 828.75 6.55 239 80 83 3 849.16 2.09 240 80 84 4 843.98 1.06 241 80 115 35 503.76 28.50 242 80 116 36 382.77 12.13 243 80 119 39 509.88 29.47 244 80 121 41 503.99 28.43 245 81 84 3 854.66 4.28 246 81 85 4 843.98 1.06 247 81 119 38 576.75 3.83 248 82 85 3 858.33 2.45 249 82 86 4 854.66 4.28 250 83 86 3 872.14 1.80 251 83 87 4 847.60 2.92 252 83 88 5 800.63 2.85 253 83 121 38 639.68 27.64 254 84 87 3 843.93 1.08 255 84 88 4 823.88 8.78 256 84 119 35 656.26 20.18 257 84 121 37 639.71 27.63 258 88 121 33 723.93 27.23 259 89 121 32 723.90 27.23 260 89 122 33 723.90 27.23 261 90 121 31 723.96 27.22 262 90 122 32 723.91 27.23 263 90 123 33 723.90 27.23 264 90 124 34 723.89 27.23 265 90 159 69 603.49 28.89 266 90 161 71 603.40 28.88 267 91 116 25 724.05 27.24 268 91 121 30 724.04 27.23 269 91 122 31 724.02 27.23 270 91 123 32 723.93 27.23 271 91 124 33 723.93 27.23 272 92 124 32 723.93 27.23 273 92 126 34 723.92 27.23 274 92 230 138 158.16 22.57 275 93 112 19 724.19 27.25 276 93 113 20 724.17 27.25 277 93 116 23 724.19 27.25 278 93 123 30 724.04 27.23 279 93 124 31 723.99 27.24 280 93 125 32 723.92 27.23 281 93 126 33 723.92 27.23 282 94 99 5 744.35 31.50 283 94 109 15 724.18 27.25 284 94 126 32 723.91 27.23 285 95 98 3 853.41 1.17 286 95 99 4 840.13 4.57 287 95 126 31 723.96 27.23 288 95 165 70 603.29 28.84 289 95 230 135 161.88 23.55 290 96 99 3 840.13 4.57 291 96 100 4 830.37 0.32 292 96 165 69 603.31 28.84 293 96 167 71 595.95 27.17 294 97 100 3 854.43 5.49 295 97 101 4 818.52 5.58 296 97 102 5 818.52 5.58 297 98 102 4 744.35 31.48 298 98 103 5 744.35 31.48 299 98 104 6 724.82 27.36 300 99 103 4 730.59 24.61 301 99 104 5 724.83 27.37 302 99 106 7 724.83 27.36 303 99 109 10 724.58 27.27 304 99 126 27 724.08 27.23 305 99 127 28 724.06 27.24 306 99 146 47 605.09 28.78 307 99 150 51 605.02 28.79 308 100 130 30 723.94 27.23 309 100 167 67 602.41 28.84 310 104 108 4 840.33 5.23 311 104 109 5 820.34 4.59 312 104 112 8 812.38 0.60 313 105 108 3 840.33 5.23 314 105 109 4 840.17 5.31 315 106 109 3 846.40 2.19 316 106 110 4 836.54 2.83 317 106 146 40 604.80 28.91 318 106 149 43 604.35 28.73 319 107 110 3 857.78 3.50 320 107 111 4 844.40 0.02 321 108 111 3 855.99 4.40 322 108 112 4 844.09 0.18 323 109 112 3 844.27 0.10 324 109 113 4 843.40 0.53 325 109 150 41 604.21 28.78 326 110 113 3 852.34 0.49 327 110 114 4 848.88 2.21 328 110 153 43 603.89 28.82 329 111 114 3 847.06 3.14 330 111 115 4 847.05 3.14 331 112 115 3 851.56 0.88 332 112 116 4 841.53 0.38 333 113 116 3 846.02 1.86 334 113 117 4 829.49 5.64 335 113 123 10 724.26 27.18 336 113 125 12 724.01 27.23 337 113 153 40 603.88 28.83 338 113 154 41 603.76 28.89 339 114 117 3 851.00 0.62 340 114 118 4 831.27 1.83 341 114 153 39 603.77 28.88 342 115 118 3 851.00 0.62 343 115 119 4 834.15 0.39 344 116 119 3 851.92 0.17 345 116 120 4 831.26 1.83 346 116 121 5 741.32 23.60 347 116 123 7 724.41 27.15 348 117 120 3 834.17 0.37 349 117 121 4 766.66 32.18 350 117 123 6 724.41 27.16 351 117 154 37 603.72 28.89 352 117 155 38 603.68 28.88 353 117 159 42 603.55 28.89 354 117 160 43 603.52 28.90 355 120 159 39 603.52 28.91 356 121 159 38 603.47 28.88 357 122 159 37 603.45 28.88 358 123 159 36 603.50 28.89 359 123 160 37 603.45 28.88 360 123 161 38 603.44 28.88 361 124 161 37 603.44 28.88 362 124 163 39 603.35 28.85 363 124 207 83 322.40 18.71 364 124 230 106 158.16 22.61 365 125 150 25 603.97 28.82 366 125 161 36 603.50 28.89 367 125 162 37 603.43 28.87 368 125 163 38 603.40 28.86 369 126 163 37 603.40 28.87 370 126 165 39 603.33 28.84 371 126 230 104 161.69 23.66 372 127 143 16 604.90 28.77 373 127 146 19 604.57 28.80 374 127 147 20 604.59 28.79 375 127 150 23 604.50 28.82 376 127 162 35 603.57 28.89 377 127 163 36 603.51 28.88 378 127 164 37 603.41 28.87 379 127 165 38 603.37 28.84 380 127 188 61 340.81 15.24 381 128 143 15 605.03 28.78 382 128 146 18 605.03 28.78 383 128 164 36 603.48 28.88 384 128 165 37 603.37 28.84 385 129 133 4 809.17 1.99 386 129 134 5 761.09 25.92 387 129 139 10 605.19 28.80 388 129 142 13 605.16 28.80 389 129 143 14 605.16 28.80 390 129 164 35 603.54 28.89 391 129 167 38 603.32 28.86 392 129 168 39 602.81 29.03 393 130 133 3 825.11 5.92 394 130 134 4 825.09 5.93 395 130 167 37 603.29 28.86 396 130 168 38 601.83 29.20 397 131 134 3 834.27 3.69 398 131 135 4 825.11 5.92 399 131 168 37 603.24 28.84 400 131 172 41 579.87 27.87 401 131 174 43 550.73 24.30 402 132 135 3 832.56 2.15 403 132 136 4 810.69 1.29 404 133 136 3 832.20 2.37 405 133 137 4 835.60 0.67 406 133 138 5 676.49 52.58 407 133 139 6 605.62 28.69 408 133 142 9 605.45 28.76 409 134 137 3 841.47 2.31 410 134 139 5 613.22 32.49 411 134 164 30 603.68 28.89 412 134 168 34 603.36 28.86 413 134 180 46 342.19 15.13 414 134 184 50 341.53 15.20 415 138 141 3 853.81 0.66 416 138 142 4 848.56 1.92 417 139 142 3 852.57 2.64 418 139 143 4 827.56 3.32 419 139 184 45 341.19 15.12 420 140 143 3 856.78 0.53 421 140 144 4 826.18 4.00 422 140 187 47 341.00 15.20 423 141 144 3 848.57 1.93 424 141 145 4 836.66 7.88 425 142 145 3 849.91 2.58 426 142 146 4 822.60 2.20 427 143 146 3 846.01 0.69 428 143 147 4 818.32 0.07 429 143 164 21 603.59 28.89 430 143 184 41 341.00 15.21 431 143 188 45 340.81 15.25 432 144 147 3 842.41 1.16 433 144 148 4 824.65 3.21 434 144 187 43 340.99 15.21 435 144 188 44 340.81 15.25 436 144 191 47 340.60 15.23 437 145 148 3 843.68 0.52 438 145 149 4 830.45 6.08 439 146 149 3 853.27 2.26 440 146 150 4 838.01 9.89 441 147 150 3 853.29 2.25 442 147 151 4 843.70 0.55 443 147 188 41 340.74 15.21 444 147 191 44 340.39 15.17 445 147 192 45 340.38 15.18 446 148 151 3 851.30 3.25 447 148 152 4 841.20 1.79 448 148 191 43 340.39 15.16 449 149 152 3 863.22 0.38 450 149 153 4 860.88 1.55 451 150 153 3 863.22 0.38 452 150 154 4 850.81 6.58 453 150 157 7 814.67 1.06 454 150 162 12 603.58 28.89 455 151 154 3 855.39 4.29 456 151 157 6 808.02 1.98 457 151 191 40 340.39 15.17 458 154 157 3 872.39 1.54 459 154 160 6 733.88 28.84 460 156 159 3 903.52 3.31 461 156 160 4 854.57 0.46 462 157 160 3 849.12 2.00 463 157 162 5 847.80 1.52 464 157 192 35 339.33 15.38 465 157 196 39 324.75 18.11 466 157 200 43 323.54 18.24 467 157 204 47 322.55 18.70 468 160 204 44 322.81 18.57 469 161 205 44 322.52 18.70 470 161 207 46 322.39 18.71 471 162 192 30 339.61 15.33 472 162 204 42 323.14 18.43 473 162 205 43 322.66 18.64 474 162 206 44 322.44 18.70 475 162 207 45 322.40 18.71 476 163 207 44 322.39 18.71 477 163 230 67 161.35 23.83 478 164 184 20 340.87 15.25 479 164 185 21 340.77 15.21 480 164 188 24 340.80 15.25 481 164 207 43 322.44 18.70 482 164 208 44 322.31 18.69 483 164 209 45 321.97 18.59 484 165 209 44 321.85 18.55 485 166 169 3 867.99 3.81 486 166 170 4 866.23 4.69 487 166 176 10 341.37 15.10 488 166 208 42 322.45 18.68 489 166 209 43 322.18 18.61 490 166 210 44 321.64 18.57 491 167 170 3 877.98 1.31 492 168 172 4 843.02 0.56 493 168 174 6 693.81 67.02 494 168 175 7 438.08 19.25 495 168 176 8 347.41 13.41 496 168 180 12 342.11 15.09 497 169 172 3 856.06 0.39 498 169 174 5 528.82 21.14 499 169 176 7 342.30 15.05 500 170 210 40 321.78 18.58 501 170 211 41 321.69 18.62 502 175 180 5 827.16 0.13 503 176 180 4 827.16 0.13 504 176 208 32 323.36 18.28 505 177 180 3 842.03 6.42 506 177 181 4 840.95 6.96 507 178 181 3 841.25 6.82 508 178 182 4 841.34 6.77 509 178 219 41 225.40 4.18 510 178 220 42 225.37 4.18 511 178 223 45 171.09 22.29 512 179 182 3 857.11 1.11 513 179 183 4 846.10 4.38 514 180 183 3 844.75 0.64 515 180 184 4 840.55 1.47 516 181 184 3 862.81 3.56 517 181 185 4 829.57 3.88 518 181 208 27 323.42 18.30 519 181 220 39 225.35 4.17 520 182 185 3 846.81 4.02 521 182 186 4 829.56 3.87 522 182 223 41 163.47 23.82 523 182 224 42 163.47 23.82 524 182 225 43 163.44 23.83 525 182 226 44 163.40 23.80 526 183 186 3 846.10 4.41 527 183 187 4 830.15 3.56 528 184 187 3 851.72 1.59 529 184 188 4 816.12 2.44 530 185 188 3 852.57 1.18 531 185 189 4 829.87 3.70 532 185 206 21 324.13 18.16 533 185 207 22 324.13 18.16 534 185 208 23 324.02 18.11 535 185 225 40 163.34 23.82 536 185 226 41 163.30 23.84 537 186 189 3 857.53 8.35 538 186 190 4 821.78 0.39 539 186 225 39 163.38 23.81 540 187 190 3 844.63 5.03 541 187 191 4 823.76 0.57 542 188 191 3 825.09 0.07 543 188 192 4 825.00 1.09 544 188 206 18 324.16 18.17 545 189 192 3 851.45 0.55 546 189 193 4 825.00 1.09 547 189 201 12 324.59 18.23 548 189 204 15 324.31 18.21 549 189 206 17 324.16 18.15 550 189 225 36 163.34 23.82 551 189 227 38 163.27 23.85 552 190 193 3 832.77 2.79 553 190 194 4 825.00 1.09 554 191 194 3 846.12 6.60 555 191 195 4 823.94 0.57 556 191 196 5 823.93 0.56 557 192 195 3 835.52 4.16 558 192 196 4 823.65 0.70 559 192 197 5 823.65 0.70 560 192 200 8 384.21 1.60 561 192 201 9 325.11 18.05 562 192 204 12 324.47 18.30 563 192 206 14 324.18 18.16 564 193 197 4 810.37 5.87 565 193 198 5 369.96 10.96 566 193 201 8 325.12 18.06 567 196 200 4 834.13 4.38 568 197 200 3 834.13 4.38 569 197 201 4 822.12 0.29 570 198 201 3 822.12 0.29 571 198 202 4 820.12 2.62 572 199 202 3 853.12 1.93 573 199 203 4 821.21 3.16 574 200 203 3 852.19 1.47 575 200 204 4 816.11 2.68 576 204 227 23 162.91 23.77 577 205 227 22 162.90 23.77 578 206 226 20 163.16 23.84 579 206 227 21 162.90 23.76 580 207 227 20 162.90 23.76 581 207 228 21 162.19 23.55 582 207 229 22 162.16 23.55 583 207 230 23 162.08 23.59 584 208 212 4 849.64 4.51 585 208 220 12 163.62 24.05 586 208 226 18 163.03 23.71 587 208 228 20 162.78 23.70 588 208 229 21 162.19 23.54 589 208 230 22 162.16 23.55 590 209 230 21 162.03 23.59 591 211 216 5 715.19 51.70 592 212 216 4 700.89 44.55 593 212 217 5 564.33 1.48 594 212 220 8 303.94 33.33 595 212 229 17 162.57 23.46 596 212 231 19 162.31 23.46 597 212 234 22 161.93 23.57 598 212 243 31 99.50 23.34 599 213 216 3 823.74 4.04 600 213 217 4 697.28 46.36 601 213 234 21 162.06 23.50 602 213 239 26 106.52 24.59 603 213 243 30 99.53 23.34 604 214 217 3 872.77 3.64 605 214 239 25 106.52 24.59 606 214 242 28 101.81 22.36 607 214 243 29 101.23 22.51 608 216 219 3 867.08 5.22 609 216 220 4 834.72 1.99 610 217 220 3 838.20 0.18 611 217 221 4 828.09 0.08 612 217 229 12 163.05 23.62 613 217 243 26 43.29 0.34 614 217 246 29 32.85 0.64 615 218 221 3 829.35 0.70 616 218 222 4 827.46 0.40 617 219 222 3 830.24 0.25 618 219 223 4 828.75 0.97 619 220 223 3 849.38 0.73 620 220 226 6 352.83 5.78 621 220 229 9 163.38 23.80 622 221 226 5 352.85 5.78 623 221 229 8 163.58 23.75 624 221 246 25 29.50 0.97 625 221 247 26 27.39 0.21 626 222 247 25 29.00 0.80 627 223 226 3 850.48 7.05 628 229 243 14 42.54 0.01 629 231 234 3 883.08 3.27 630 231 235 4 825.91 1.46 631 231 240 9 56.62 0.31 632 231 243 12 44.04 0.72 633 232 235 3 850.81 1.84 634 233 236 3 867.30 2.43 635 233 237 4 854.31 0.10 636 234 237 3 851.77 1.36 637 234 239 5 396.01 28.63 638 234 240 6 345.89 23.50 639 234 243 9 295.36 38.55 640 235 240 5 360.15 16.37 641 237 240 3 859.84 2.49 642 238 241 3 867.22 1.16 643 239 242 3 843.66 5.53 644 239 243 4 827.07 1.03 645 240 243 3 828.59 1.79 646 240 244 4 824.11 0.45 647 241 244 3 831.47 0.56 648 241 245 4 824.14 0.43 649 242 245 3 842.33 6.82 650 242 246 4 824.11 0.45 651 243 246 3 824.10 0.45 652 245 248 3 868.32 0.76