BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 203 200 177.96 1.06 2 3 204 201 171.83 0.64 3 3 205 202 164.39 0.42 4 3 224 221 117.59 0.01 5 3 225 222 117.56 0.00 6 3 227 224 117.39 0.01 7 4 35 31 500.96 24.78 8 4 36 32 500.94 24.79 9 4 37 33 500.95 24.78 10 4 205 201 174.44 0.67 11 5 36 31 500.95 24.78 12 5 37 32 500.96 24.78 13 5 227 222 117.53 0.01 14 5 228 223 117.51 0.01 15 5 229 224 117.52 0.01 16 5 243 238 44.56 0.50 17 5 244 239 42.45 1.18 18 5 246 241 38.33 1.72 19 5 247 242 25.07 0.55 20 6 37 31 500.97 24.78 21 6 39 33 501.03 24.75 22 6 90 84 352.47 0.35 23 6 124 118 332.98 0.07 24 6 161 155 332.08 0.52 25 6 205 199 183.02 1.77 26 6 207 201 170.21 0.15 27 6 227 221 117.59 0.01 28 6 228 222 117.53 0.01 29 6 229 223 117.52 0.01 30 7 37 30 501.05 24.75 31 7 38 31 501.00 24.75 32 7 39 32 501.04 24.74 33 7 229 222 117.53 0.01 34 7 231 224 117.50 0.01 35 7 240 233 54.69 1.16 36 7 243 236 44.74 0.55 37 7 244 237 41.38 0.70 38 8 39 31 501.04 24.74 39 8 229 221 117.59 0.01 40 8 230 222 117.52 0.01 41 8 231 223 117.51 0.01 42 8 240 232 55.05 1.07 43 9 39 30 501.13 24.71 44 9 40 31 501.04 24.74 45 9 41 32 501.13 24.69 46 9 231 222 117.52 0.01 47 9 235 226 76.31 0.41 48 9 240 231 55.05 1.07 49 10 41 31 501.15 24.69 50 10 64 54 453.93 1.64 51 10 92 82 354.51 0.16 52 10 95 85 353.53 0.17 53 10 230 220 118.28 0.07 54 10 231 221 117.59 0.01 55 10 232 222 117.50 0.01 56 10 235 225 83.72 0.10 57 11 14 3 864.82 1.06 58 11 20 9 504.53 23.41 59 11 23 12 504.23 23.27 60 11 40 29 501.19 24.71 61 11 41 30 501.19 24.71 62 11 42 31 501.18 24.70 63 11 43 32 501.21 24.66 64 11 64 53 454.28 1.52 65 11 235 224 111.10 2.17 66 11 236 225 82.50 0.50 67 12 43 31 501.25 24.67 68 12 64 52 453.94 1.66 69 12 95 83 355.10 0.20 70 12 97 85 355.31 0.22 71 12 170 158 314.99 0.52 72 12 232 220 118.28 0.07 73 13 43 30 501.29 24.66 74 14 43 29 501.37 24.62 75 14 236 222 117.43 0.03 76 15 19 4 826.03 5.44 77 15 20 5 814.47 0.35 78 15 46 31 501.78 24.41 79 16 19 3 851.48 0.12 80 16 20 4 832.01 2.44 81 16 46 30 501.81 24.39 82 17 20 3 858.44 3.36 83 17 21 4 832.00 2.43 84 17 46 29 501.82 24.39 85 17 49 32 492.63 19.79 86 18 21 3 846.24 2.50 87 18 22 4 840.82 0.02 88 19 22 3 850.84 0.21 89 19 23 4 840.75 0.06 90 20 23 3 855.91 2.08 91 20 24 4 840.75 0.06 92 20 46 26 502.01 24.15 93 20 49 29 470.81 8.43 94 20 50 30 469.96 8.08 95 21 24 3 870.27 4.22 96 21 25 4 845.32 2.37 97 21 49 28 470.84 8.44 98 21 53 32 460.05 3.02 99 22 25 3 861.50 0.17 100 22 26 4 845.33 2.37 101 23 26 3 850.59 0.14 102 23 27 4 854.85 2.40 103 23 236 213 76.32 0.38 104 24 27 3 854.85 2.40 105 24 28 4 836.30 0.22 106 24 40 16 502.48 24.16 107 24 53 29 457.75 0.66 108 24 54 30 457.62 0.59 109 25 28 3 840.17 1.73 110 25 29 4 832.26 2.12 111 26 29 3 857.61 3.64 112 26 30 4 837.88 0.71 113 26 237 211 76.30 0.36 114 26 238 212 68.48 1.75 115 26 241 215 56.75 0.85 116 27 30 3 856.18 3.12 117 27 40 13 503.33 23.78 118 27 56 29 457.58 0.56 119 27 59 32 457.14 0.62 120 27 240 213 55.69 1.39 121 27 241 214 54.70 1.16 122 27 244 217 52.35 0.23 123 28 53 25 457.76 0.65 124 28 54 26 457.66 0.60 125 28 55 27 457.61 0.58 126 28 56 28 457.58 0.56 127 30 56 26 476.76 9.04 128 30 241 211 54.55 1.10 129 31 245 214 44.88 0.55 130 32 36 4 852.69 1.48 131 32 38 6 691.36 2.42 132 32 56 24 502.78 22.07 133 32 58 26 499.95 20.84 134 32 241 209 54.50 1.11 135 32 244 212 44.84 0.54 136 32 245 213 44.76 0.57 137 33 36 3 880.44 2.02 138 33 58 25 506.16 23.94 139 33 245 212 43.10 0.93 140 33 248 215 23.10 0.50 141 34 58 24 506.13 23.95 142 36 58 22 506.05 24.03 143 36 244 208 42.52 1.22 144 37 58 21 506.03 24.04 145 37 90 53 354.09 0.03 146 37 205 168 184.13 1.39 147 38 58 20 506.06 24.05 148 38 59 21 506.03 24.05 149 38 60 22 506.03 24.05 150 38 240 202 54.77 1.18 151 38 244 206 42.39 1.21 152 39 60 21 505.86 24.12 153 39 62 23 505.78 24.16 154 39 90 51 354.09 0.03 155 39 124 85 349.84 1.59 156 40 54 14 506.52 23.89 157 40 59 19 506.26 24.01 158 40 60 20 506.12 24.07 159 40 61 21 505.82 24.16 160 40 62 22 505.79 24.16 161 40 240 200 55.54 0.84 162 41 62 21 505.80 24.17 163 41 64 23 505.66 24.21 164 41 92 51 355.12 0.33 165 42 46 4 845.62 1.13 166 42 47 5 553.69 0.55 167 42 50 8 553.38 0.70 168 42 61 19 506.26 24.01 169 42 62 20 506.03 24.10 170 42 64 22 505.74 24.17 171 43 46 3 862.01 2.73 172 43 47 4 846.82 0.53 173 43 64 21 505.74 24.17 174 44 47 3 853.60 3.93 175 44 78 34 360.89 0.33 176 46 49 3 857.09 3.92 177 46 50 4 842.80 0.39 178 47 50 3 854.25 5.33 179 47 51 4 840.48 1.55 180 47 61 14 506.34 24.00 181 47 62 15 506.33 24.00 182 47 63 16 506.22 23.96 183 47 83 36 359.79 0.42 184 47 86 39 359.88 0.45 185 47 88 41 358.35 0.28 186 48 51 3 852.35 4.38 187 48 52 4 848.13 1.22 188 48 86 38 359.81 0.42 189 49 52 3 851.83 1.49 190 49 53 4 850.25 2.27 191 50 53 3 864.50 0.95 192 50 54 4 850.26 2.27 193 50 61 11 506.40 24.03 194 51 54 3 869.41 0.85 195 51 61 10 506.44 24.02 196 51 86 35 359.79 0.43 197 51 87 36 356.72 1.09 198 51 88 37 355.81 0.64 199 54 59 5 506.54 24.03 200 54 61 7 506.48 24.04 201 56 59 3 858.62 2.23 202 60 89 29 355.69 0.58 203 60 90 30 355.68 0.59 204 61 88 27 355.80 0.57 205 61 89 28 355.76 0.58 206 61 90 29 355.68 0.59 207 62 88 26 355.95 0.57 208 62 90 28 355.67 0.59 209 63 83 20 359.80 0.42 210 63 90 27 355.86 0.55 211 63 91 28 355.78 0.56 212 63 92 29 355.80 0.55 213 64 92 28 355.80 0.54 214 65 78 13 360.92 0.33 215 65 91 26 357.76 0.40 216 65 92 27 356.31 0.72 217 65 93 28 355.98 0.56 218 65 98 33 356.84 0.68 219 65 104 39 358.67 0.66 220 65 112 47 360.35 0.26 221 66 78 12 360.92 0.33 222 66 80 14 360.69 0.32 223 66 91 25 359.58 0.38 224 66 93 27 357.87 1.05 225 66 112 46 360.47 0.26 226 67 74 7 362.14 0.47 227 67 77 10 361.08 0.33 228 67 78 11 361.06 0.32 229 67 108 41 360.62 0.33 230 67 112 45 360.62 0.32 231 68 108 40 360.64 0.33 232 68 111 43 360.67 0.33 233 68 112 44 360.64 0.32 234 68 115 47 360.78 0.29 235 69 74 5 757.72 25.38 236 69 115 46 360.87 0.30 237 70 79 9 360.15 0.94 238 70 81 11 361.20 0.49 239 70 115 45 361.24 0.48 240 71 74 3 976.10 1.46 241 74 77 3 874.51 0.30 242 79 82 3 839.38 0.87 243 79 83 4 831.77 1.50 244 80 83 3 846.00 4.16 245 80 84 4 834.26 5.41 246 80 91 11 415.17 22.37 247 80 115 35 461.47 0.42 248 80 116 36 461.29 0.51 249 80 119 39 519.28 23.27 250 80 121 41 513.95 25.80 251 81 84 3 851.59 1.22 252 81 85 4 847.12 1.01 253 81 119 38 574.62 0.64 254 82 85 3 847.12 1.02 255 82 86 4 847.12 1.01 256 83 86 3 855.66 3.25 257 83 87 4 847.12 1.01 258 83 88 5 682.92 4.21 259 83 90 7 639.52 16.82 260 83 91 8 572.47 3.21 261 83 121 38 604.99 0.47 262 84 87 3 835.00 3.48 263 84 88 4 749.25 35.24 264 84 119 35 609.44 1.72 265 84 120 36 609.43 1.72 266 84 121 37 605.00 0.47 267 89 121 32 662.96 1.02 268 89 122 33 662.94 1.02 269 90 121 31 662.98 1.02 270 90 122 32 662.95 1.02 271 90 123 33 662.94 1.02 272 90 124 34 662.94 1.02 273 90 159 69 662.95 1.02 274 90 161 71 662.94 1.02 275 91 116 25 663.11 1.01 276 91 121 30 663.06 1.03 277 91 122 31 663.01 1.02 278 91 123 32 662.96 1.02 279 91 124 33 662.95 1.02 280 92 95 3 904.30 0.28 281 92 124 32 662.95 1.02 282 92 126 34 662.95 1.02 283 93 109 16 663.13 1.03 284 93 112 19 663.25 1.06 285 93 113 20 663.16 1.02 286 93 116 23 663.31 1.04 287 93 123 30 663.08 1.02 288 93 124 31 663.05 1.03 289 93 125 32 662.98 1.02 290 93 126 33 662.97 1.02 291 94 99 5 759.20 34.51 292 94 109 15 663.15 1.02 293 94 126 32 662.98 1.02 294 95 98 3 854.88 5.92 295 95 99 4 838.89 2.10 296 95 126 31 663.02 1.02 297 95 165 70 663.00 1.02 298 96 99 3 843.43 0.17 299 96 100 4 838.89 2.10 300 96 167 71 648.11 2.62 301 96 170 74 537.07 17.63 302 97 100 3 862.75 5.24 303 97 101 4 838.88 2.10 304 97 102 5 831.93 1.39 305 97 170 73 597.40 2.28 306 98 102 4 766.98 31.09 307 98 103 5 766.97 31.09 308 98 104 6 664.29 0.97 309 99 103 4 664.52 0.89 310 99 104 5 664.32 0.96 311 99 106 7 664.32 0.96 312 99 109 10 664.31 0.97 313 99 126 27 663.28 1.02 314 99 127 28 663.24 1.01 315 99 146 47 663.43 0.98 316 100 103 3 844.88 1.11 317 104 108 4 835.38 4.06 318 104 109 5 829.69 1.19 319 104 112 8 818.80 1.58 320 105 108 3 845.72 1.10 321 105 109 4 835.97 3.77 322 106 109 3 847.94 2.21 323 106 110 4 836.00 3.75 324 106 145 39 663.58 1.01 325 106 146 40 663.52 1.03 326 106 149 43 663.74 0.95 327 107 110 3 849.64 3.08 328 107 111 4 831.54 0.27 329 107 153 46 665.04 0.70 330 108 111 3 855.10 6.32 331 108 112 4 837.16 0.99 332 109 112 3 849.62 2.89 333 109 113 4 835.01 2.06 334 109 125 16 663.63 0.98 335 110 113 3 857.19 0.90 336 110 114 4 849.78 3.17 337 110 149 39 663.79 0.94 338 110 150 40 663.76 0.94 339 110 153 43 665.12 0.66 340 111 114 3 858.16 0.40 341 111 115 4 847.61 4.25 342 112 115 3 850.41 3.48 343 112 116 4 842.52 6.79 344 113 116 3 859.60 1.70 345 113 117 4 842.50 6.80 346 113 123 10 664.61 0.87 347 113 125 12 663.77 0.94 348 113 150 37 665.10 0.66 349 113 154 41 665.50 0.52 350 114 117 3 856.76 0.65 351 114 118 4 843.05 0.41 352 114 153 39 665.51 0.53 353 114 154 40 665.50 0.53 354 115 118 3 844.84 0.48 355 115 119 4 842.32 0.04 356 116 119 3 862.63 3.60 357 116 120 4 842.11 0.15 358 116 121 5 818.62 1.06 359 116 123 7 686.99 8.85 360 117 120 3 842.82 0.49 361 117 121 4 822.56 3.02 362 117 123 6 687.02 8.85 363 117 154 37 701.09 5.61 364 117 156 39 724.78 0.46 365 117 159 42 701.08 5.61 366 117 160 43 695.79 4.50 367 122 159 37 796.41 0.99 368 123 159 36 796.56 1.01 369 123 160 37 796.47 0.98 370 123 161 38 796.48 1.01 371 124 161 37 796.50 0.99 372 124 163 39 796.47 1.01 373 124 230 106 118.25 0.04 374 125 147 22 796.75 0.98 375 125 150 25 796.69 0.99 376 125 157 32 796.63 1.00 377 125 160 35 796.60 1.01 378 125 161 36 796.57 1.01 379 125 162 37 796.50 1.00 380 125 163 38 796.48 1.01 381 126 163 37 796.50 1.00 382 126 165 39 796.48 1.01 383 126 230 104 118.87 0.31 384 127 143 16 796.93 1.01 385 127 146 19 796.88 0.98 386 127 147 20 796.88 0.98 387 127 162 35 796.60 1.01 388 127 163 36 796.57 1.01 389 127 164 37 796.51 1.01 390 127 165 38 796.50 1.01 391 127 188 61 255.79 1.47 392 128 142 14 797.03 0.96 393 128 143 15 796.96 1.00 394 128 146 18 796.94 1.01 395 128 164 36 796.56 1.02 396 128 165 37 796.55 1.02 397 128 167 39 796.04 1.14 398 129 133 4 841.18 3.48 399 129 134 5 824.59 1.11 400 129 139 10 797.10 0.98 401 129 142 13 797.04 0.96 402 129 164 35 796.61 1.00 403 129 166 37 796.50 1.00 404 129 167 38 796.47 1.01 405 129 168 39 796.47 1.01 406 130 133 3 847.36 0.43 407 130 134 4 847.33 0.42 408 130 167 37 796.49 1.01 409 130 168 38 796.49 1.01 410 131 134 3 855.83 3.81 411 131 135 4 855.83 3.81 412 131 168 37 796.49 1.01 413 132 135 3 855.87 3.80 414 132 136 4 855.84 3.81 415 133 136 3 855.87 3.81 416 133 137 4 855.84 3.81 417 133 138 5 797.21 0.96 418 133 139 6 797.21 0.97 419 133 142 9 797.20 0.96 420 134 137 3 855.84 3.81 421 134 139 5 805.73 3.29 422 134 168 34 796.58 1.01 423 138 141 3 864.80 8.49 424 138 142 4 842.93 1.39 425 139 142 3 851.05 1.70 426 139 143 4 846.07 0.18 427 139 164 25 796.64 1.01 428 139 184 45 256.30 1.36 429 140 143 3 850.67 0.99 430 140 144 4 842.97 1.41 431 140 184 44 256.30 1.36 432 140 187 47 256.07 1.47 433 141 144 3 858.86 6.23 434 141 145 4 853.37 8.97 435 142 145 3 860.58 5.37 436 142 146 4 856.70 7.30 437 143 146 3 856.70 7.30 438 143 147 4 843.99 0.95 439 143 164 21 796.59 1.00 440 143 188 45 255.86 1.48 441 144 147 3 848.29 1.19 442 144 148 4 836.87 2.60 443 144 191 47 255.89 1.49 444 145 148 3 851.32 0.33 445 145 149 4 846.08 0.10 446 146 149 3 872.25 0.45 447 146 150 4 845.33 0.48 448 147 150 3 860.41 5.47 449 147 151 4 839.18 0.45 450 147 157 10 797.33 0.88 451 147 162 15 796.62 0.99 452 147 188 41 255.90 1.46 453 147 191 44 255.83 1.48 454 147 192 45 255.56 1.56 455 148 151 3 847.69 0.90 456 148 152 4 837.25 0.51 457 148 191 43 255.89 1.49 458 148 195 47 249.24 2.00 459 149 153 4 837.24 0.51 460 150 153 3 853.20 7.48 461 150 154 4 822.65 6.19 462 150 157 7 798.30 1.09 463 151 154 3 846.08 11.04 464 151 157 6 798.29 1.09 465 151 191 40 255.87 1.49 466 151 192 41 255.56 1.56 467 151 195 44 238.15 1.79 468 151 196 45 233.34 0.01 469 154 157 3 894.02 1.70 470 154 160 6 797.44 0.94 471 157 160 3 831.19 9.48 472 157 162 5 847.20 1.47 473 157 196 39 201.96 1.43 474 160 204 44 200.59 1.30 475 161 204 43 200.61 1.29 476 161 205 44 200.56 1.28 477 161 207 46 200.45 1.31 478 162 204 42 200.78 1.23 479 162 205 43 200.64 1.28 480 162 206 44 200.48 1.30 481 162 207 45 200.45 1.31 482 163 207 44 200.45 1.31 483 163 230 67 118.27 0.04 484 164 184 20 255.87 1.50 485 164 185 21 255.76 1.46 486 164 188 24 255.72 1.46 487 164 207 43 200.62 1.29 488 164 208 44 200.46 1.32 489 164 209 45 200.46 1.32 490 165 170 5 821.16 9.30 491 165 209 44 200.45 1.31 492 165 230 65 119.27 0.45 493 166 169 3 866.26 3.62 494 166 170 4 849.41 4.80 495 166 176 10 256.75 1.53 496 166 208 42 200.66 1.28 497 166 209 43 200.62 1.29 498 167 170 3 866.26 3.62 499 168 172 4 844.17 2.41 500 168 174 6 265.21 2.08 501 168 175 7 258.30 1.37 502 168 176 8 258.27 1.36 503 169 172 3 851.20 1.10 504 169 174 5 258.27 1.35 505 169 175 6 258.23 1.33 506 169 208 39 201.15 1.42 507 169 210 41 200.28 1.33 508 169 211 42 200.28 1.32 509 170 210 40 200.28 1.32 510 172 211 39 200.40 1.38 511 173 211 38 200.40 1.38 512 174 211 37 200.52 1.33 513 175 208 33 201.25 1.37 514 175 210 35 200.61 1.30 515 175 211 36 200.52 1.33 516 175 216 41 148.30 0.88 517 176 181 5 806.44 0.34 518 176 208 32 201.25 1.37 519 176 220 44 123.13 0.02 520 177 180 3 858.59 0.98 521 177 181 4 830.44 1.12 522 178 181 3 842.43 5.08 523 178 182 4 833.01 2.41 524 178 219 41 121.98 0.04 525 178 220 42 121.70 0.07 526 178 223 45 121.59 0.16 527 179 182 3 869.42 4.99 528 179 183 4 842.25 5.19 529 179 223 44 121.67 0.12 530 180 183 3 863.91 7.75 531 180 184 4 842.25 5.19 532 181 184 3 851.97 2.45 533 181 185 4 843.85 4.39 534 181 208 27 201.36 1.41 535 181 220 39 121.69 0.07 536 181 226 45 120.88 0.36 537 182 185 3 852.42 2.68 538 182 186 4 844.83 6.49 539 182 223 41 120.94 0.35 540 182 224 42 120.95 0.35 541 182 225 43 120.88 0.36 542 182 226 44 120.81 0.33 543 183 186 3 852.44 2.68 544 183 187 4 847.76 5.02 545 184 187 3 853.64 4.45 546 184 188 4 847.76 5.02 547 185 188 3 851.28 3.27 548 185 189 4 846.58 5.62 549 185 206 21 201.57 1.36 550 185 207 22 201.56 1.36 551 185 208 23 201.38 1.41 552 185 225 40 120.73 0.30 553 185 226 41 120.50 0.41 554 186 189 3 855.58 1.12 555 186 190 4 846.59 5.62 556 186 225 39 120.49 0.42 557 187 190 3 854.23 3.62 558 187 191 4 836.63 0.52 559 188 191 3 848.53 6.46 560 188 192 4 835.77 0.06 561 188 206 18 201.57 1.37 562 189 192 3 856.50 2.48 563 189 193 4 846.29 0.24 564 189 201 12 202.13 1.38 565 189 204 15 201.84 1.32 566 189 206 17 201.57 1.37 567 189 225 36 120.49 0.42 568 189 227 38 120.41 0.45 569 190 193 3 864.26 1.90 570 190 194 4 854.98 1.71 571 191 194 3 865.33 1.38 572 191 195 4 847.06 5.67 573 192 195 3 845.04 4.33 574 192 196 4 836.06 0.18 575 192 201 9 202.21 1.41 576 192 204 12 201.82 1.30 577 193 196 3 850.15 4.11 578 193 197 4 699.01 60.54 579 193 198 5 272.72 8.43 580 193 201 8 206.90 0.93 581 196 200 4 818.68 5.71 582 197 200 3 872.58 5.22 583 197 201 4 835.21 1.33 584 198 201 3 851.88 3.28 585 198 202 4 834.00 1.93 586 199 202 3 849.82 2.04 587 199 203 4 841.82 1.97 588 200 203 3 854.36 1.66 589 200 204 4 841.80 1.98 590 201 204 3 841.93 2.10 591 204 227 23 120.19 0.51 592 205 227 22 119.78 0.71 593 206 225 19 120.62 0.36 594 206 226 20 120.45 0.43 595 206 227 21 120.23 0.49 596 207 227 20 120.27 0.52 597 207 228 21 119.59 0.62 598 207 229 22 119.25 0.45 599 207 230 23 119.23 0.45 600 208 220 12 120.72 0.42 601 208 226 18 120.64 0.36 602 208 228 20 120.28 0.52 603 208 229 21 119.78 0.70 604 208 230 22 119.27 0.45 605 209 230 21 119.27 0.45 606 211 216 5 852.43 2.91 607 212 216 4 852.43 2.91 608 212 229 17 120.82 0.44 609 212 231 19 120.28 0.53 610 212 234 22 119.18 0.48 611 212 243 31 53.59 1.56 612 213 216 3 864.36 0.58 613 213 217 4 842.34 2.14 614 213 234 21 119.17 0.49 615 213 239 26 55.54 0.83 616 213 243 30 54.52 1.19 617 214 239 25 54.76 1.11 618 214 242 28 54.54 1.19 619 214 243 29 54.43 1.16 620 215 218 3 888.36 1.24 621 216 219 3 868.15 4.98 622 216 220 4 865.81 6.15 623 217 220 3 876.74 0.69 624 217 221 4 865.65 6.23 625 217 229 12 121.02 0.42 626 217 243 26 47.23 0.15 627 217 246 29 38.22 2.83 628 218 221 3 862.01 4.41 629 218 246 28 37.66 2.57 630 220 223 3 845.29 3.19 631 220 226 6 121.16 0.44 632 220 229 9 121.09 0.45 633 220 247 27 37.51 2.62 634 221 246 25 37.54 2.62 635 221 247 26 37.12 2.52 636 223 226 3 846.78 6.05 637 226 247 21 37.12 2.52 638 227 247 20 31.55 0.42 639 229 243 14 44.66 0.51 640 231 234 3 874.80 2.21 641 231 235 4 816.93 8.62 642 231 240 9 86.66 17.09 643 231 243 12 50.88 0.83 644 232 235 3 873.03 1.33 645 233 236 3 879.28 0.62 646 234 237 3 847.22 1.51 647 234 239 5 366.82 49.29 648 234 240 6 122.26 0.60 649 234 243 9 86.95 17.07 650 235 240 5 122.31 0.59 651 237 240 3 876.96 0.16 652 238 241 3 879.57 1.15 653 239 242 3 854.62 0.42 654 239 243 4 836.62 0.17 655 240 243 3 848.99 0.58 656 240 244 4 836.62 0.17 657 241 244 3 856.29 5.41 658 241 245 4 836.60 0.18 659 242 245 3 845.86 4.79 660 242 246 4 823.47 2.23 661 243 246 3 851.62 0.75 662 245 248 3 813.71 7.02