BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 118.09 0.47 2 3 228 225 116.64 0.56 3 3 229 226 113.36 0.69 4 4 204 200 144.50 12.46 5 4 205 201 140.16 10.38 6 4 206 202 140.10 10.41 7 4 207 203 139.56 10.19 8 4 229 225 118.75 0.22 9 5 36 31 671.55 2.21 10 5 37 32 671.57 2.18 11 5 38 33 671.58 2.18 12 5 207 202 142.82 11.77 13 6 36 30 671.62 2.19 14 6 37 31 671.59 2.19 15 6 38 32 671.58 2.18 16 6 223 217 119.01 0.14 17 6 229 223 118.98 0.12 18 6 249 243 24.01 0.90 19 7 37 30 671.62 2.19 20 7 38 31 671.58 2.18 21 7 40 33 671.59 2.18 22 7 163 156 152.85 15.45 23 7 207 200 151.88 15.71 24 7 209 202 144.18 12.46 25 7 229 222 119.11 0.17 26 7 230 223 118.99 0.12 27 7 231 224 118.98 0.13 28 8 37 29 671.71 2.18 29 8 38 30 671.70 2.18 30 8 39 31 671.60 2.18 31 8 40 32 671.59 2.18 32 8 231 223 118.99 0.12 33 8 242 234 54.50 1.18 34 8 246 238 41.95 1.09 35 9 40 31 671.60 2.18 36 9 124 115 155.29 15.94 37 9 209 200 152.13 15.81 38 9 231 222 119.08 0.15 39 9 232 223 118.97 0.12 40 9 233 224 118.95 0.12 41 9 242 233 54.52 1.18 42 10 40 30 671.68 2.17 43 10 41 31 671.63 2.18 44 10 42 32 671.62 2.19 45 10 233 223 118.99 0.12 46 10 237 227 88.05 0.06 47 10 242 232 54.52 1.18 48 11 42 31 671.64 2.18 49 11 65 54 458.46 0.29 50 11 95 84 155.63 15.93 51 11 232 221 119.73 0.23 52 11 233 222 119.00 0.12 53 11 234 223 118.98 0.11 54 11 237 226 91.78 0.69 55 12 15 3 892.08 0.96 56 12 21 9 672.78 2.14 57 12 24 12 672.66 2.19 58 12 25 13 672.66 2.20 59 12 28 16 672.60 2.22 60 12 41 29 671.74 2.17 61 12 42 30 671.69 2.17 62 12 43 31 671.66 2.17 63 12 51 39 576.76 9.88 64 12 237 225 113.14 0.44 65 12 238 226 89.39 0.49 66 13 44 31 671.65 2.16 67 13 65 52 459.59 0.83 68 13 95 82 156.19 16.02 69 13 97 84 155.84 15.85 70 13 234 221 119.56 0.23 71 14 44 30 671.69 2.17 72 15 44 29 671.74 2.18 73 15 238 223 118.92 0.08 74 16 21 5 828.01 0.33 75 16 47 31 671.63 2.16 76 17 20 3 872.12 3.56 77 17 21 4 839.36 4.91 78 17 47 30 671.62 2.17 79 18 21 3 874.16 2.54 80 18 22 4 840.44 4.37 81 18 50 32 625.57 0.18 82 19 22 3 874.64 2.31 83 19 23 4 857.08 3.96 84 20 23 3 858.28 4.54 85 20 24 4 858.28 4.54 86 21 24 3 858.30 4.54 87 21 25 4 858.28 4.54 88 21 47 26 671.61 2.14 89 21 50 29 598.91 0.03 90 21 238 217 110.58 1.00 91 22 25 3 858.30 4.54 92 22 26 4 858.29 4.54 93 22 50 28 598.93 0.04 94 22 54 32 463.10 1.12 95 23 26 3 866.11 0.61 96 23 27 4 852.88 7.23 97 24 27 3 867.07 1.80 98 24 28 4 846.11 2.54 99 24 237 213 111.37 0.62 100 24 238 214 106.00 2.58 101 24 239 215 90.67 0.15 102 24 240 216 88.14 0.10 103 25 28 3 847.36 1.92 104 25 29 4 846.11 2.54 105 25 51 26 475.70 0.10 106 25 54 29 462.75 1.07 107 25 55 30 461.76 0.79 108 26 29 3 859.80 2.12 109 26 30 4 848.29 3.63 110 26 54 28 462.92 1.16 111 27 30 3 859.61 2.50 112 27 31 4 848.29 3.63 113 27 240 213 84.86 0.96 114 27 243 216 66.46 0.89 115 28 31 3 856.31 4.15 116 28 41 13 671.84 2.14 117 28 55 27 461.75 0.79 118 28 57 29 461.27 0.86 119 28 60 32 461.12 0.88 120 28 237 209 109.30 1.02 121 28 242 214 66.39 0.93 122 28 243 215 54.63 1.24 123 29 54 25 462.00 0.71 124 29 55 26 461.75 0.79 125 29 56 27 461.66 0.81 126 29 57 28 461.26 0.86 127 31 57 26 461.27 0.88 128 31 243 212 54.45 1.21 129 31 247 216 50.80 0.10 130 32 247 215 50.73 0.06 131 33 37 4 887.80 2.17 132 33 39 6 672.66 2.48 133 33 57 24 461.21 0.86 134 33 59 26 461.11 0.88 135 33 60 27 461.11 0.88 136 33 246 213 50.10 0.35 137 34 59 25 461.11 0.88 138 35 59 24 461.09 0.88 139 35 246 211 50.04 0.37 140 35 249 214 34.95 0.24 141 35 250 215 26.75 0.41 142 37 246 209 41.35 0.78 143 37 249 212 30.55 1.68 144 38 59 21 460.11 0.96 145 38 60 22 459.92 0.88 146 38 61 23 459.87 0.87 147 38 90 52 158.95 16.23 148 39 59 20 460.14 0.97 149 39 60 21 459.92 0.88 150 39 61 22 459.92 0.88 151 39 246 207 47.04 0.59 152 40 61 21 459.93 0.88 153 40 63 23 459.84 0.86 154 40 90 50 158.97 16.23 155 40 124 84 155.24 15.96 156 41 51 10 461.81 0.72 157 41 55 14 461.01 0.86 158 41 60 19 460.80 0.97 159 41 61 20 460.37 0.89 160 41 62 21 459.93 0.88 161 41 63 22 459.88 0.89 162 42 63 21 459.89 0.89 163 42 65 23 459.73 0.84 164 42 92 50 159.81 16.70 165 43 47 4 839.72 4.03 166 43 48 5 742.75 36.80 167 43 51 8 471.00 2.66 168 43 63 20 460.09 0.96 169 43 65 22 459.86 0.88 170 44 47 3 857.39 3.50 171 44 48 4 848.41 0.94 172 44 65 21 460.03 0.93 173 45 48 3 868.67 1.72 174 45 64 19 460.30 0.85 175 45 66 21 460.09 0.96 176 45 78 33 209.01 2.66 177 45 83 38 208.93 2.62 178 47 50 3 877.11 1.70 179 47 51 4 863.58 1.66 180 48 51 3 863.57 1.67 181 48 52 4 863.50 1.71 182 48 62 14 461.15 0.83 183 48 63 15 461.13 0.83 184 48 64 16 461.00 0.88 185 48 83 35 208.59 2.60 186 48 86 38 208.59 2.60 187 48 88 40 203.06 2.46 188 49 52 3 881.68 0.83 189 49 53 4 863.50 1.71 190 49 86 37 208.36 2.48 191 50 53 3 876.10 3.62 192 50 54 4 863.47 1.73 193 51 54 3 875.32 4.19 194 51 55 4 853.53 6.71 195 51 62 11 461.17 0.84 196 52 62 10 461.18 0.84 197 52 86 34 208.30 2.50 198 55 60 5 512.71 24.88 199 55 62 7 461.19 0.87 200 57 60 3 867.51 3.68 201 60 246 186 50.05 0.41 202 61 90 29 159.37 16.20 203 62 88 26 160.37 16.55 204 62 90 28 160.07 16.55 205 63 88 25 161.64 16.15 206 63 90 27 159.50 16.26 207 63 91 28 160.27 16.57 208 64 83 19 207.95 2.33 209 64 88 24 195.11 0.19 210 64 90 26 161.19 16.14 211 64 91 27 160.37 16.55 212 64 92 28 161.25 16.11 213 65 92 27 161.58 16.18 214 66 83 17 208.84 2.56 215 66 91 25 161.92 16.27 216 66 92 26 161.78 16.22 217 66 93 27 161.78 16.22 218 66 98 32 162.06 16.34 219 66 112 46 201.74 3.12 220 67 78 11 209.11 2.54 221 67 79 12 208.97 2.60 222 67 80 13 208.90 2.60 223 67 83 16 208.95 2.61 224 67 91 24 189.02 3.04 225 67 93 26 194.71 0.15 226 67 112 45 202.10 3.10 227 68 74 6 261.75 23.55 228 68 77 9 209.39 2.48 229 68 78 10 209.38 2.49 230 68 79 11 209.06 2.64 231 68 80 12 209.06 2.64 232 68 112 44 206.41 2.86 233 69 80 11 209.13 2.60 234 69 81 12 209.41 2.50 235 69 115 46 207.74 2.81 236 70 74 4 928.52 0.32 237 70 79 9 209.40 2.50 238 70 81 11 209.42 2.50 239 71 74 3 967.14 0.93 240 71 79 8 209.46 2.50 241 71 81 10 209.48 2.52 242 71 82 11 209.48 2.52 243 74 77 3 873.71 1.41 244 78 82 4 852.53 2.84 245 78 83 5 830.12 1.27 246 79 82 3 863.57 2.05 247 79 83 4 853.76 3.46 248 80 83 3 859.00 0.83 249 80 84 4 848.56 4.83 250 80 91 11 335.84 10.75 251 80 115 35 392.42 39.03 252 80 116 36 335.95 10.79 253 80 121 41 471.78 0.20 254 81 84 3 856.73 2.32 255 81 85 4 845.19 3.14 256 82 85 3 871.77 4.67 257 82 86 4 848.05 1.71 258 83 86 3 848.37 1.86 259 83 87 4 848.05 1.71 260 83 88 5 755.35 29.01 261 83 91 8 472.55 0.17 262 83 121 38 492.87 7.01 263 84 87 3 859.60 0.07 264 84 88 4 828.99 5.48 265 84 119 35 498.56 4.38 266 84 120 36 493.94 6.72 267 84 121 37 492.93 7.03 268 88 121 33 551.01 0.18 269 89 120 31 551.02 0.18 270 89 121 32 551.00 0.20 271 89 122 33 550.97 0.20 272 90 121 31 551.02 0.18 273 90 122 32 550.99 0.19 274 90 124 34 550.96 0.21 275 91 116 25 551.13 0.20 276 91 121 30 551.11 0.20 277 91 122 31 551.03 0.18 278 91 123 32 551.01 0.18 279 91 124 33 550.98 0.21 280 92 95 3 938.55 0.33 281 92 124 32 551.00 0.20 282 92 126 34 550.97 0.21 283 92 232 140 123.04 1.36 284 93 112 19 551.19 0.21 285 93 113 20 551.12 0.21 286 93 116 23 551.26 0.18 287 93 123 30 551.09 0.19 288 93 124 31 551.04 0.19 289 93 125 32 551.00 0.19 290 93 126 33 550.98 0.21 291 94 99 5 844.05 0.10 292 94 109 15 551.11 0.21 293 94 126 32 551.00 0.19 294 95 98 3 865.17 6.01 295 95 99 4 853.54 0.21 296 95 126 31 551.03 0.20 297 95 167 72 550.93 0.23 298 95 232 137 124.33 0.96 299 96 99 3 853.55 0.20 300 96 100 4 853.55 0.21 301 96 167 71 550.93 0.21 302 96 169 73 547.97 0.94 303 97 100 3 860.91 3.83 304 97 101 4 853.52 0.20 305 97 102 5 853.52 0.20 306 98 101 3 863.90 2.35 307 98 102 4 836.40 3.29 308 98 103 5 834.71 2.45 309 98 104 6 559.09 4.04 310 99 104 5 559.09 4.04 311 99 106 7 559.09 4.04 312 99 109 10 553.29 1.10 313 99 126 27 551.26 0.14 314 99 127 28 551.23 0.15 315 99 146 47 551.36 0.18 316 103 108 5 849.09 4.09 317 104 108 4 849.09 4.09 318 104 109 5 837.11 1.04 319 104 112 8 829.08 2.37 320 105 108 3 874.13 1.23 321 105 109 4 858.85 0.79 322 106 109 3 865.67 3.01 323 106 110 4 860.02 0.19 324 106 145 39 551.64 0.12 325 106 146 40 551.43 0.15 326 106 149 43 551.74 0.09 327 107 110 3 864.49 3.60 328 107 111 4 858.84 0.79 329 108 111 3 859.92 0.24 330 108 112 4 859.92 0.23 331 109 112 3 859.93 0.23 332 109 113 4 851.81 3.83 333 109 150 41 551.89 0.14 334 110 113 3 868.87 1.78 335 110 114 4 852.40 3.53 336 110 149 39 551.89 0.15 337 110 150 40 551.88 0.14 338 110 153 43 552.75 0.24 339 111 114 3 865.95 3.24 340 111 115 4 858.04 0.71 341 111 153 42 552.84 0.19 342 112 115 3 864.31 4.07 343 112 116 4 852.79 4.76 344 113 116 3 863.80 0.73 345 113 117 4 860.49 0.91 346 113 123 10 552.11 0.18 347 113 125 12 551.79 0.17 348 113 150 37 552.18 0.21 349 113 154 41 562.25 4.35 350 114 117 3 876.50 3.95 351 114 118 4 860.49 0.91 352 114 153 39 552.89 0.18 353 114 154 40 553.05 0.14 354 115 118 3 876.50 3.95 355 115 119 4 861.16 1.24 356 116 119 3 874.02 5.19 357 116 120 4 861.16 1.24 358 116 121 5 562.69 4.13 359 116 123 7 562.24 4.33 360 117 120 3 861.18 1.24 361 117 123 6 562.41 4.25 362 117 154 37 632.53 30.52 363 117 158 41 632.50 30.49 364 117 161 44 562.62 4.15 365 117 162 45 562.62 4.15 366 120 161 41 796.93 1.64 367 121 161 40 796.96 1.64 368 122 160 38 797.04 1.61 369 122 161 39 796.96 1.63 370 122 207 85 295.36 0.41 371 123 161 38 797.02 1.61 372 123 162 39 796.97 1.63 373 123 163 40 796.97 1.63 374 124 163 39 796.97 1.63 375 124 165 41 796.87 1.66 376 124 209 85 294.78 0.64 377 125 150 25 797.21 1.63 378 125 163 38 797.02 1.62 379 125 164 39 796.96 1.64 380 125 165 40 796.96 1.64 381 126 165 39 796.96 1.64 382 126 167 41 796.90 1.65 383 126 232 106 124.08 0.88 384 127 143 16 797.47 1.62 385 127 146 19 797.37 1.62 386 127 150 23 797.36 1.62 387 127 164 37 797.05 1.61 388 127 165 38 796.99 1.63 389 127 166 39 796.97 1.63 390 127 167 40 796.97 1.63 391 127 190 63 299.20 0.20 392 128 143 15 797.49 1.61 393 128 146 18 797.53 1.65 394 128 166 38 796.98 1.63 395 128 167 39 796.97 1.63 396 128 169 41 796.83 1.65 397 129 133 4 838.44 3.58 398 129 134 5 821.40 3.97 399 129 139 10 797.63 1.65 400 129 142 13 797.61 1.65 401 129 143 14 797.57 1.64 402 129 166 37 797.03 1.63 403 129 169 40 796.88 1.66 404 129 170 41 796.81 1.64 405 130 133 3 868.09 5.69 406 130 134 4 843.05 6.82 407 130 169 39 796.89 1.67 408 130 170 40 796.80 1.63 409 131 134 3 845.33 5.69 410 131 135 4 843.06 6.82 411 131 170 39 796.87 1.67 412 131 174 43 736.07 1.56 413 131 176 45 632.82 28.78 414 132 135 3 881.66 9.63 415 132 136 4 847.57 4.57 416 133 136 3 890.21 5.36 417 133 137 4 870.58 4.45 418 133 138 5 799.78 2.55 419 133 142 9 797.82 1.59 420 134 137 3 875.36 2.06 421 134 138 4 825.77 7.38 422 134 139 5 797.83 1.62 423 134 166 32 797.14 1.62 424 134 170 36 796.85 1.66 425 134 182 48 317.30 8.50 426 138 141 3 872.56 0.67 427 138 142 4 849.72 1.15 428 139 142 3 881.94 4.13 429 139 143 4 852.34 2.46 430 140 143 3 870.12 1.42 431 140 144 4 851.87 2.25 432 140 189 49 299.45 0.18 433 141 144 3 873.65 0.35 434 141 145 4 859.23 1.42 435 142 145 3 861.84 2.73 436 142 146 4 859.30 1.46 437 143 146 3 867.27 5.45 438 143 147 4 859.30 1.46 439 143 166 23 797.02 1.60 440 143 189 46 299.30 0.21 441 143 190 47 299.23 0.19 442 144 147 3 867.48 5.34 443 144 148 4 853.83 4.21 444 144 189 45 299.31 0.23 445 144 193 49 299.19 0.20 446 145 148 3 857.28 5.00 447 145 149 4 853.83 4.20 448 146 149 3 870.91 1.81 449 146 150 4 854.11 4.05 450 147 150 3 863.87 0.86 451 147 151 4 857.51 2.32 452 147 159 12 797.62 1.54 453 147 164 17 797.08 1.62 454 147 193 46 299.06 0.20 455 148 151 3 861.97 1.77 456 148 152 4 848.25 2.31 457 148 193 45 299.03 0.21 458 149 152 3 873.34 3.17 459 150 153 3 898.82 6.34 460 150 154 4 857.74 2.43 461 151 154 3 878.88 3.62 462 151 159 8 797.74 1.51 463 151 193 42 299.02 0.21 464 151 198 47 296.73 0.26 465 154 158 4 847.48 2.54 466 154 159 5 843.30 4.62 467 154 162 8 797.63 1.56 468 155 158 3 863.98 0.75 469 155 159 4 862.05 2.71 470 156 159 3 892.87 0.74 471 156 198 42 296.52 0.36 472 156 201 45 296.41 0.35 473 156 202 46 296.22 0.43 474 157 160 3 895.74 0.69 475 158 161 3 886.86 2.26 476 159 162 3 848.05 2.71 477 159 198 39 296.50 0.36 478 159 202 43 296.05 0.39 479 159 206 47 295.64 0.48 480 162 206 44 295.58 0.48 481 163 207 44 295.35 0.41 482 163 209 46 294.79 0.64 483 164 206 42 295.73 0.52 484 164 207 43 295.62 0.50 485 164 208 44 294.89 0.62 486 164 209 45 294.79 0.64 487 165 209 44 294.78 0.64 488 165 232 67 123.86 0.99 489 166 170 4 1072.50 0.00 490 166 186 20 299.27 0.23 491 166 187 21 299.22 0.19 492 166 190 24 299.19 0.19 493 166 209 43 295.33 0.42 494 166 210 44 294.74 0.65 495 166 211 45 294.60 0.63 496 167 211 44 294.30 0.66 497 168 171 3 873.39 1.68 498 168 172 4 852.49 4.78 499 168 178 10 300.04 0.21 500 168 210 42 295.31 0.43 501 168 211 43 294.75 0.63 502 168 212 44 294.48 0.60 503 169 172 3 854.49 3.78 504 169 174 5 849.85 3.47 505 170 174 4 842.35 0.28 506 170 176 6 521.34 26.18 507 170 178 8 323.33 5.52 508 170 182 12 317.23 8.54 509 171 174 3 864.43 1.22 510 171 176 5 373.75 9.36 511 171 178 7 323.33 5.51 512 172 212 40 294.49 0.60 513 177 182 5 850.99 2.04 514 178 182 4 834.24 6.33 515 178 210 32 295.61 0.54 516 179 182 3 851.88 2.13 517 179 183 4 847.10 0.09 518 180 183 3 852.25 1.77 519 180 184 4 844.86 1.03 520 180 221 41 245.02 2.70 521 180 222 42 197.31 12.86 522 180 225 45 189.36 16.73 523 181 184 3 855.14 2.57 524 181 185 4 845.08 1.14 525 182 185 3 860.14 0.07 526 182 186 4 847.70 0.79 527 183 186 3 860.14 0.07 528 183 187 4 847.72 0.78 529 183 210 27 295.67 0.54 530 183 222 39 197.28 12.87 531 184 187 3 848.58 0.35 532 184 188 4 851.34 0.10 533 184 225 41 134.72 5.66 534 184 226 42 134.74 5.67 535 184 227 43 134.56 5.61 536 185 188 3 853.32 0.90 537 185 189 4 855.70 2.27 538 186 189 3 858.96 0.65 539 186 190 4 855.69 2.27 540 187 190 3 859.43 0.41 541 187 191 4 850.41 2.01 542 187 208 21 296.06 0.46 543 187 209 22 296.06 0.46 544 187 210 23 295.88 0.48 545 187 227 40 132.35 4.75 546 187 228 41 131.34 4.25 547 188 191 3 858.11 1.84 548 188 192 4 848.95 1.32 549 188 227 39 132.58 4.63 550 189 192 3 854.72 0.14 551 189 193 4 842.34 2.06 552 190 193 3 864.55 0.29 553 190 194 4 853.74 0.25 554 190 208 18 295.95 0.51 555 191 194 3 863.94 0.59 556 191 195 4 840.88 6.68 557 191 203 12 296.75 0.35 558 191 206 15 296.39 0.41 559 191 208 17 296.05 0.46 560 191 227 36 132.34 4.75 561 191 229 38 132.34 4.75 562 192 195 3 861.00 3.38 563 192 196 4 834.60 3.54 564 193 196 3 857.55 5.08 565 193 197 4 834.49 3.60 566 193 198 5 832.01 2.36 567 194 197 3 863.39 5.07 568 194 198 4 818.94 4.16 569 194 199 5 818.92 4.16 570 194 202 8 347.10 0.10 571 194 203 9 306.81 1.78 572 194 206 12 300.62 1.69 573 195 199 4 646.74 81.93 574 195 200 5 369.60 10.17 575 195 203 8 306.82 1.79 576 198 202 4 854.46 0.35 577 199 202 3 859.51 2.17 578 199 203 4 852.34 0.71 579 200 203 3 857.74 1.96 580 200 204 4 852.34 0.71 581 201 204 3 869.59 2.72 582 201 205 4 856.30 2.69 583 202 205 3 864.68 1.50 584 202 206 4 856.27 2.68 585 203 206 3 858.53 1.55 586 206 229 23 128.93 3.21 587 207 229 22 128.88 3.21 588 208 227 19 129.53 3.35 589 208 229 21 128.98 3.16 590 209 229 20 128.85 3.09 591 209 230 21 124.84 1.17 592 209 231 22 124.78 1.14 593 209 232 23 124.70 1.18 594 210 213 3 1072.50 0.00 595 210 214 4 892.38 3.27 596 210 222 12 139.25 8.31 597 210 228 18 129.50 3.34 598 210 230 20 128.77 3.04 599 210 231 21 124.80 1.14 600 210 232 22 124.79 1.14 601 211 232 21 124.67 1.08 602 213 218 5 856.84 0.85 603 214 218 4 852.37 1.39 604 214 219 5 529.71 28.11 605 214 222 8 358.56 1.39 606 214 231 17 125.06 1.09 607 214 236 22 124.64 1.06 608 214 245 31 53.26 1.36 609 215 218 3 883.21 3.98 610 215 219 4 852.37 1.38 611 215 236 21 124.72 1.10 612 215 241 26 57.99 2.64 613 215 245 30 53.44 1.45 614 216 220 4 881.03 2.89 615 216 241 25 57.73 2.76 616 216 244 28 54.26 1.18 617 216 245 29 53.44 1.46 618 217 220 3 943.33 1.51 619 218 221 3 868.10 2.68 620 218 222 4 843.56 0.10 621 219 222 3 856.83 2.25 622 219 223 4 853.99 0.83 623 219 231 12 132.66 4.63 624 219 245 26 47.77 0.77 625 219 248 29 36.29 0.91 626 220 223 3 856.30 1.32 627 220 224 4 843.89 0.09 628 220 248 28 33.88 0.29 629 221 224 3 858.47 0.24 630 221 225 4 859.56 0.31 631 222 225 3 882.84 5.07 632 222 228 6 303.78 0.18 633 222 231 9 302.30 0.42 634 223 228 5 312.42 4.61 635 223 229 6 303.67 0.24 636 223 230 7 302.42 0.38 637 223 231 8 302.41 0.38 638 223 248 25 33.87 0.29 639 223 249 26 31.46 1.10 640 223 250 27 32.87 0.43 641 225 228 3 859.32 0.36 642 228 231 3 1022.57 2.18 643 230 249 19 24.07 0.93 644 231 245 14 49.70 0.31 645 231 249 18 32.39 3.07 646 233 236 3 893.52 1.57 647 233 237 4 824.63 6.59 648 233 242 9 138.25 5.04 649 233 245 12 51.09 0.03 650 234 237 3 874.77 0.87 651 234 238 4 874.77 0.87 652 235 238 3 894.80 0.90 653 235 239 4 865.73 3.25 654 236 239 3 865.72 2.35 655 236 241 5 352.29 0.73 656 236 242 6 314.29 6.79 657 236 245 9 214.50 43.07 658 237 242 5 314.29 6.79 659 239 242 3 873.09 0.35 660 240 243 3 892.43 0.26 661 241 244 3 875.67 8.12 662 241 245 4 853.07 1.67 663 242 245 3 854.27 2.04 664 242 246 4 852.81 1.54 665 243 246 3 855.73 0.07 666 243 247 4 852.98 1.62 667 244 247 3 853.18 1.53 668 244 248 4 853.01 1.60 669 245 248 3 862.76 3.09 670 245 249 4 859.53 4.71 671 246 249 3 879.21 2.71 672 247 250 3 890.53 2.30