BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 113.31 0.70 2 3 228 225 111.76 1.46 3 3 229 226 110.45 0.99 4 4 204 200 178.25 0.07 5 4 205 201 178.05 0.18 6 4 206 202 176.08 0.82 7 4 207 203 165.03 0.89 8 4 229 225 116.05 0.57 9 5 36 31 506.84 22.74 10 5 37 32 506.81 22.75 11 5 38 33 506.80 22.75 12 5 207 202 176.07 0.82 13 6 36 30 506.91 22.73 14 6 37 31 506.81 22.74 15 6 38 32 506.82 22.74 16 6 223 217 116.56 0.48 17 6 229 223 116.53 0.47 18 6 249 243 23.97 0.98 19 7 37 30 506.89 22.73 20 7 38 31 506.83 22.75 21 7 40 33 506.81 22.75 22 7 90 83 271.21 41.04 23 7 163 156 264.47 40.35 24 7 207 200 182.49 0.58 25 7 209 202 171.84 1.30 26 7 229 222 116.64 0.49 27 7 230 223 116.53 0.47 28 7 231 224 116.41 0.48 29 8 37 29 506.93 22.73 30 8 38 30 506.88 22.73 31 8 39 31 506.82 22.75 32 8 40 32 506.82 22.74 33 8 231 223 116.56 0.48 34 8 242 234 60.09 0.36 35 8 246 238 39.63 0.29 36 9 40 31 506.82 22.74 37 9 124 115 269.48 41.18 38 9 209 200 187.29 1.23 39 9 231 222 118.00 0.22 40 9 232 223 116.42 0.49 41 9 233 224 116.39 0.48 42 9 242 233 60.30 0.37 43 10 40 30 506.91 22.73 44 10 41 31 506.88 22.72 45 10 42 32 506.88 22.71 46 10 233 223 116.53 0.47 47 10 237 227 87.16 0.02 48 10 242 232 60.32 0.38 49 11 42 31 506.90 22.71 50 11 65 54 458.52 0.42 51 11 95 84 269.79 41.15 52 11 232 221 119.00 0.12 53 11 233 222 116.59 0.47 54 11 234 223 116.41 0.48 55 11 237 226 91.53 0.59 56 12 15 3 884.74 4.63 57 12 21 9 508.09 22.68 58 12 24 12 508.06 22.67 59 12 25 13 508.05 22.68 60 12 28 16 508.03 22.66 61 12 41 29 507.02 22.68 62 12 42 30 507.00 22.68 63 12 43 31 506.94 22.70 64 12 65 53 459.65 0.80 65 12 237 225 111.23 1.40 66 12 238 226 89.62 0.35 67 13 44 31 507.00 22.67 68 13 65 52 459.44 0.86 69 13 95 82 271.22 41.05 70 13 97 84 270.57 40.80 71 13 234 221 118.99 0.12 72 14 44 30 507.01 22.68 73 15 44 29 507.02 22.68 74 15 238 223 116.30 0.47 75 16 20 4 843.62 2.78 76 16 21 5 834.38 1.02 77 16 47 31 506.99 22.57 78 17 20 3 868.56 5.34 79 17 21 4 842.34 3.42 80 18 21 3 873.37 2.15 81 18 22 4 843.62 2.78 82 18 50 32 489.37 13.70 83 19 22 3 871.46 3.90 84 19 23 4 868.95 0.79 85 20 23 3 868.96 0.80 86 20 24 4 868.96 0.80 87 21 24 3 868.96 0.80 88 21 25 4 868.94 0.79 89 21 47 26 506.97 22.55 90 21 238 217 110.45 0.94 91 22 25 3 875.31 2.39 92 22 26 4 868.93 0.79 93 22 54 32 462.97 1.05 94 23 26 3 876.33 2.83 95 23 27 4 868.91 0.80 96 24 27 3 867.13 1.77 97 24 28 4 867.02 1.74 98 24 237 213 111.25 0.57 99 24 238 214 104.25 0.86 100 24 239 215 97.27 1.15 101 25 28 3 855.60 6.04 102 25 29 4 853.53 5.01 103 25 51 26 462.99 1.06 104 25 54 29 462.97 1.12 105 25 55 30 462.89 1.15 106 26 29 3 853.53 5.01 107 26 30 4 853.51 5.00 108 26 54 28 463.08 1.19 109 27 30 3 859.61 2.50 110 27 31 4 848.27 3.63 111 27 240 213 77.92 0.47 112 27 243 216 64.36 0.29 113 28 31 3 857.27 4.63 114 28 41 13 508.74 21.82 115 28 55 27 462.99 1.28 116 28 57 29 462.50 1.54 117 28 60 32 462.42 1.54 118 28 237 209 109.94 0.72 119 28 242 214 61.91 0.91 120 28 243 215 60.25 0.35 121 29 54 25 463.11 1.21 122 29 55 26 462.87 1.34 123 29 56 27 462.80 1.39 124 29 57 28 462.50 1.53 125 31 57 26 462.56 1.56 126 31 243 212 59.54 0.32 127 33 37 4 887.80 2.17 128 33 39 6 565.50 0.67 129 33 57 24 506.15 23.40 130 33 59 26 490.13 15.39 131 33 60 27 490.13 15.39 132 33 246 213 47.84 0.03 133 34 59 25 511.03 25.84 134 35 59 24 512.50 26.59 135 35 246 211 45.13 0.98 136 35 249 214 36.70 0.69 137 35 250 215 24.37 0.78 138 37 59 22 512.11 26.91 139 37 246 209 39.94 0.15 140 37 249 212 26.18 0.27 141 38 59 21 512.14 26.92 142 38 61 23 512.03 26.89 143 38 90 52 271.23 41.05 144 39 59 20 512.36 26.84 145 39 60 21 512.11 26.91 146 39 61 22 512.05 26.91 147 39 246 207 39.87 0.21 148 40 61 21 512.12 26.91 149 40 63 23 511.91 26.89 150 40 90 50 271.23 41.05 151 40 124 84 269.21 41.06 152 41 51 10 513.47 26.41 153 41 55 14 512.70 26.79 154 41 60 19 512.65 26.76 155 41 61 20 512.36 26.86 156 41 62 21 512.09 26.91 157 41 63 22 512.02 26.89 158 42 63 21 512.02 26.89 159 42 65 23 511.86 26.91 160 42 92 50 271.93 41.34 161 43 47 4 750.56 43.45 162 43 48 5 689.24 67.14 163 43 51 8 560.63 2.83 164 43 63 20 512.36 26.89 165 43 65 22 512.00 26.88 166 44 47 3 848.46 0.96 167 44 48 4 848.41 0.94 168 44 65 21 512.13 26.95 169 45 48 3 868.67 1.72 170 45 64 19 512.42 26.88 171 45 66 21 512.37 26.86 172 45 78 33 289.59 37.99 173 45 83 38 288.35 37.69 174 47 50 3 877.12 1.70 175 47 51 4 853.98 3.14 176 48 51 3 880.26 0.13 177 48 52 4 863.60 1.67 178 48 62 14 512.87 26.70 179 48 63 15 512.85 26.70 180 48 64 16 512.73 26.73 181 48 83 35 277.29 41.95 182 48 86 38 276.45 42.07 183 48 88 40 275.17 42.09 184 49 52 3 880.26 0.12 185 49 53 4 863.52 1.72 186 49 86 37 275.74 42.43 187 50 53 3 876.10 3.62 188 50 54 4 863.52 1.72 189 51 54 3 875.33 4.17 190 51 55 4 863.51 1.73 191 51 62 11 512.85 26.69 192 52 62 10 512.84 26.69 193 52 86 34 275.77 42.40 194 55 60 5 512.99 26.74 195 55 62 7 512.91 26.73 196 57 60 3 867.51 3.68 197 60 242 182 61.69 0.81 198 60 246 186 47.38 0.29 199 61 90 29 271.63 41.24 200 62 87 25 272.95 41.16 201 62 88 26 272.53 41.16 202 62 90 28 271.87 41.37 203 63 88 25 272.93 41.14 204 63 90 27 271.87 41.37 205 63 91 28 271.95 41.33 206 64 83 19 276.37 42.01 207 64 88 24 273.72 40.81 208 64 90 26 272.63 41.12 209 64 91 27 272.17 41.34 210 64 92 28 272.64 41.11 211 65 92 27 272.45 41.20 212 66 91 25 273.96 40.86 213 66 92 26 273.72 40.74 214 66 93 27 273.66 40.71 215 66 98 32 273.88 40.66 216 66 112 46 274.57 40.73 217 67 78 11 289.72 37.98 218 67 79 12 289.63 37.97 219 67 80 13 289.69 37.93 220 67 83 16 289.57 37.98 221 67 91 24 274.31 40.83 222 67 93 26 274.25 40.79 223 67 112 45 275.08 40.98 224 68 74 6 292.38 36.84 225 68 77 9 289.91 38.04 226 68 78 10 289.78 37.98 227 68 79 11 289.73 37.95 228 68 80 12 289.76 37.95 229 68 112 44 275.43 40.81 230 69 80 11 289.80 37.96 231 69 81 12 289.99 38.05 232 69 115 46 277.75 40.51 233 70 74 4 900.90 1.09 234 70 79 9 289.96 38.04 235 70 81 11 289.99 38.05 236 71 79 8 289.99 38.05 237 71 81 10 289.99 38.05 238 74 77 3 871.53 1.03 239 78 82 4 850.39 0.22 240 78 83 5 832.59 4.88 241 79 82 3 857.86 0.27 242 79 83 4 855.75 2.46 243 80 83 3 859.00 0.84 244 80 84 4 854.52 1.84 245 80 91 11 392.47 38.86 246 80 115 35 403.36 34.00 247 80 116 36 393.06 39.15 248 80 121 41 524.54 25.95 249 81 84 3 860.40 0.48 250 81 85 4 838.23 2.12 251 82 85 3 868.20 4.37 252 82 86 4 840.69 0.89 253 83 86 3 854.13 2.80 254 83 87 4 838.24 2.12 255 83 88 5 823.12 8.40 256 83 91 8 565.63 5.74 257 83 121 38 582.06 0.02 258 84 87 3 852.00 3.88 259 84 88 4 838.19 2.14 260 84 119 35 582.06 0.02 261 84 121 37 582.07 0.02 262 88 121 33 659.58 0.30 263 89 120 31 659.69 0.29 264 89 121 32 659.54 0.32 265 89 122 33 659.52 0.32 266 90 121 31 659.65 0.31 267 90 122 32 659.52 0.32 268 90 124 34 659.52 0.32 269 90 163 73 659.53 0.32 270 91 116 25 659.78 0.29 271 91 121 30 659.77 0.29 272 91 122 31 659.69 0.31 273 91 123 32 659.60 0.29 274 91 124 33 659.54 0.32 275 92 124 32 659.60 0.30 276 92 126 34 659.52 0.33 277 92 232 140 120.38 0.03 278 93 112 19 659.87 0.27 279 93 113 20 659.78 0.29 280 93 116 23 659.88 0.28 281 93 123 30 659.77 0.29 282 93 124 31 659.75 0.29 283 93 125 32 659.62 0.32 284 93 126 33 659.53 0.33 285 94 99 5 764.14 30.62 286 94 109 15 659.76 0.29 287 94 126 32 659.57 0.35 288 95 98 3 848.50 2.31 289 95 99 4 848.52 2.30 290 95 126 31 659.58 0.35 291 95 167 72 659.53 0.36 292 95 232 137 121.69 0.41 293 96 99 3 848.75 2.19 294 96 100 4 851.13 0.99 295 96 167 71 659.53 0.36 296 96 169 73 657.28 0.73 297 97 100 3 851.14 0.99 298 97 101 4 851.14 0.99 299 97 102 5 837.28 5.93 300 98 101 3 869.94 1.57 301 98 102 4 834.64 4.62 302 98 103 5 834.09 4.52 303 98 104 6 661.95 0.50 304 99 104 5 660.37 0.29 305 99 106 7 667.28 3.17 306 99 109 10 660.35 0.30 307 99 126 27 659.84 0.28 308 99 127 28 659.81 0.28 309 99 146 47 659.86 0.27 310 103 108 5 858.82 0.77 311 104 108 4 850.38 3.46 312 104 109 5 838.25 2.60 313 104 112 8 811.45 5.54 314 105 108 3 874.13 1.23 315 105 109 4 858.86 0.79 316 106 109 3 865.52 3.08 317 106 110 4 858.86 0.79 318 106 145 39 660.45 0.42 319 106 146 40 660.01 0.33 320 106 149 43 660.48 0.42 321 107 110 3 864.50 3.60 322 107 111 4 858.85 0.79 323 108 111 3 858.32 1.05 324 108 112 4 859.94 0.24 325 109 112 3 859.94 0.24 326 109 113 4 851.38 4.04 327 109 150 41 660.54 0.44 328 110 113 3 868.88 1.78 329 110 114 4 852.40 3.54 330 110 149 39 660.83 0.36 331 110 150 40 660.49 0.42 332 110 153 43 661.75 0.74 333 111 114 3 868.88 1.78 334 111 115 4 856.39 0.10 335 111 153 42 661.88 0.71 336 112 115 3 868.88 1.78 337 112 116 4 851.91 4.32 338 113 116 3 863.78 0.73 339 113 117 4 859.62 0.48 340 113 123 10 661.71 0.77 341 113 125 12 660.37 0.38 342 113 150 37 661.67 0.75 343 113 154 41 662.01 0.67 344 113 162 49 661.57 0.52 345 114 117 3 876.49 3.94 346 114 118 4 859.63 0.48 347 114 153 39 661.97 0.69 348 114 154 40 661.98 0.69 349 115 118 3 874.00 5.19 350 115 119 4 861.15 1.24 351 116 119 3 874.01 5.19 352 116 120 4 861.16 1.24 353 116 121 5 671.23 3.79 354 116 123 7 662.11 0.74 355 117 120 3 862.98 0.34 356 117 123 6 662.15 0.74 357 117 154 37 695.47 7.81 358 117 158 41 695.48 7.80 359 117 161 44 674.44 2.71 360 117 162 45 670.26 4.79 361 120 161 41 796.89 1.64 362 121 161 40 796.95 1.64 363 122 160 38 796.99 1.64 364 122 161 39 796.95 1.64 365 122 207 85 246.18 24.19 366 123 161 38 796.98 1.64 367 123 162 39 796.95 1.64 368 123 163 40 796.95 1.64 369 124 163 39 796.96 1.64 370 124 165 41 796.83 1.68 371 124 209 85 244.64 23.78 372 125 150 25 797.14 1.61 373 125 163 38 796.98 1.64 374 125 164 39 796.96 1.64 375 125 165 40 796.95 1.64 376 126 165 39 796.95 1.64 377 126 167 41 796.90 1.66 378 126 232 106 121.58 0.39 379 127 143 16 797.52 1.66 380 127 146 19 797.33 1.61 381 127 147 20 797.32 1.61 382 127 150 23 797.35 1.62 383 127 164 37 797.03 1.62 384 127 165 38 796.98 1.63 385 127 166 39 796.96 1.64 386 127 167 40 796.95 1.64 387 127 190 63 257.41 20.82 388 128 143 15 797.54 1.66 389 128 146 18 797.53 1.66 390 128 166 38 796.98 1.63 391 128 167 39 796.95 1.64 392 128 169 41 796.79 1.63 393 129 133 4 835.88 3.74 394 129 134 5 810.70 1.38 395 129 139 10 797.62 1.69 396 129 142 13 797.64 1.68 397 129 143 14 797.64 1.68 398 129 166 37 797.00 1.64 399 129 169 40 796.89 1.67 400 129 170 41 796.81 1.64 401 130 133 3 864.10 3.69 402 130 134 4 850.44 3.14 403 130 169 39 796.89 1.67 404 130 170 40 796.77 1.61 405 131 134 3 864.97 4.12 406 131 135 4 850.45 3.14 407 131 170 39 796.87 1.67 408 131 174 43 733.78 2.73 409 131 176 45 702.95 2.14 410 132 135 3 881.67 9.63 411 132 136 4 850.43 3.13 412 133 136 3 866.83 2.20 413 133 137 4 859.56 1.44 414 133 138 5 797.87 1.60 415 133 139 6 797.83 1.62 416 133 142 9 797.79 1.61 417 134 137 3 861.90 4.66 418 134 139 5 797.81 1.64 419 134 166 32 797.12 1.64 420 134 170 36 796.85 1.66 421 138 141 3 872.48 3.50 422 138 142 4 834.12 8.92 423 139 142 3 864.15 1.23 424 139 143 4 847.41 2.27 425 140 143 3 870.12 1.42 426 140 144 4 841.96 0.46 427 140 189 49 258.02 20.87 428 141 145 4 849.24 3.57 429 142 145 3 861.84 2.73 430 142 146 4 859.23 1.43 431 143 146 3 867.27 5.45 432 143 147 4 858.77 1.20 433 143 166 23 797.02 1.60 434 143 189 46 257.92 20.82 435 144 147 3 867.26 5.45 436 144 148 4 852.53 1.92 437 144 189 45 257.92 20.81 438 144 190 46 257.76 20.78 439 144 193 49 257.48 20.83 440 145 148 3 861.82 2.73 441 145 149 4 851.58 1.44 442 146 149 3 870.91 1.81 443 146 150 4 851.58 1.44 444 147 150 3 862.28 0.07 445 147 151 4 848.26 2.32 446 147 159 12 797.63 1.51 447 147 164 17 797.06 1.62 448 147 193 46 257.30 20.75 449 147 194 47 257.28 20.73 450 148 151 3 853.02 4.70 451 148 152 4 848.26 2.32 452 148 193 45 257.31 20.73 453 149 152 3 874.08 2.79 454 150 153 3 898.82 6.34 455 150 154 4 864.80 1.10 456 151 154 3 878.88 3.62 457 151 159 8 797.66 1.51 458 151 193 42 257.30 20.74 459 151 198 47 248.27 23.86 460 154 158 4 853.52 0.24 461 154 159 5 853.52 0.24 462 154 162 8 797.75 1.52 463 155 158 3 862.10 3.70 464 155 159 4 861.98 3.89 465 156 159 3 879.17 3.17 466 156 198 42 248.09 23.84 467 156 201 45 247.99 23.80 468 156 202 46 247.78 23.73 469 157 160 3 895.74 0.69 470 158 161 3 886.86 2.26 471 158 162 4 870.50 0.24 472 159 162 3 865.75 2.13 473 159 164 5 873.09 1.54 474 159 198 39 248.04 23.84 475 159 202 43 246.94 24.06 476 159 206 47 246.31 24.19 477 162 206 44 246.28 24.20 478 163 207 44 246.02 24.27 479 163 209 46 244.68 23.76 480 164 190 26 257.28 20.76 481 164 206 42 246.44 24.12 482 164 207 43 246.22 24.21 483 164 208 44 244.74 23.75 484 164 209 45 244.68 23.76 485 165 209 44 244.66 23.77 486 165 232 67 121.47 0.49 487 166 186 20 257.61 20.89 488 166 187 21 257.52 20.86 489 166 190 24 257.38 20.80 490 166 208 42 246.21 24.19 491 166 209 43 245.86 24.32 492 166 210 44 244.64 23.78 493 166 211 45 244.56 23.75 494 167 172 5 844.72 7.75 495 167 211 44 244.56 23.75 496 168 171 3 884.08 0.05 497 168 172 4 863.06 1.42 498 168 210 42 245.86 24.32 499 168 211 43 244.69 23.75 500 168 212 44 244.56 23.75 501 169 172 3 862.75 0.32 502 170 174 4 826.98 4.64 503 170 176 6 425.91 29.19 504 170 178 8 287.66 12.28 505 170 182 12 258.40 20.89 506 171 174 3 868.37 3.19 507 171 176 5 306.14 19.57 508 171 178 7 258.52 20.86 509 177 182 5 854.69 0.19 510 178 182 4 847.13 0.07 511 178 210 32 246.58 23.98 512 179 182 3 858.34 0.62 513 179 183 4 847.18 0.06 514 180 183 3 854.46 0.67 515 180 184 4 847.48 0.21 516 180 221 41 186.09 32.12 517 180 222 42 172.28 25.24 518 180 225 45 125.26 1.73 519 181 184 3 855.13 2.56 520 181 185 4 851.12 1.60 521 182 185 3 857.16 1.42 522 182 186 4 851.13 1.60 523 183 186 3 870.58 5.73 524 183 187 4 848.59 0.35 525 183 210 27 246.53 24.01 526 183 222 39 162.19 20.20 527 184 187 3 854.64 1.79 528 184 188 4 848.95 1.28 529 184 225 41 122.80 0.59 530 184 226 42 122.80 0.59 531 184 227 43 122.78 0.59 532 185 188 3 854.53 0.28 533 185 189 4 851.33 0.10 534 186 189 3 857.66 1.28 535 186 190 4 851.33 0.09 536 187 190 3 855.69 2.27 537 187 191 4 848.88 1.30 538 187 208 21 247.00 23.91 539 187 209 22 246.78 24.02 540 187 210 23 246.69 24.02 541 187 227 40 122.62 0.61 542 187 228 41 122.59 0.61 543 188 191 3 860.43 3.00 544 188 192 4 847.06 2.26 545 188 227 39 122.61 0.62 546 189 192 3 859.01 3.71 547 189 193 4 851.25 0.17 548 190 193 3 865.21 0.62 549 190 194 4 855.07 0.42 550 190 208 18 247.00 23.91 551 191 194 3 864.61 0.92 552 191 195 4 855.07 0.42 553 191 203 12 248.15 23.87 554 191 206 15 247.53 23.80 555 191 208 17 247.02 23.91 556 191 227 36 122.58 0.62 557 191 229 38 122.56 0.62 558 192 195 3 860.34 3.05 559 192 196 4 852.41 0.42 560 193 196 3 860.95 3.38 561 193 197 4 850.83 1.14 562 193 198 5 849.71 0.58 563 194 197 3 858.15 4.78 564 194 198 4 817.12 3.31 565 194 199 5 817.11 3.31 566 194 202 8 300.17 24.55 567 194 203 9 255.38 27.48 568 194 206 12 247.57 23.75 569 195 199 4 807.02 2.21 570 195 200 5 301.58 25.30 571 195 203 8 253.34 26.47 572 198 202 4 840.29 2.81 573 199 202 3 864.34 4.58 574 199 203 4 848.42 0.30 575 200 203 3 857.74 1.96 576 200 204 4 848.42 0.30 577 201 204 3 859.62 1.02 578 201 205 4 857.73 1.96 579 202 205 3 858.53 1.55 580 202 206 4 848.43 0.30 581 203 206 3 858.53 1.55 582 206 229 23 122.31 0.57 583 207 229 22 122.31 0.57 584 208 227 19 122.59 0.59 585 208 228 20 122.38 0.55 586 208 229 21 122.36 0.55 587 209 229 20 122.38 0.55 588 209 230 21 122.27 0.55 589 209 231 22 121.83 0.34 590 209 232 23 121.55 0.48 591 210 222 12 122.41 0.54 592 210 228 18 122.60 0.58 593 210 230 20 122.40 0.54 594 210 231 21 122.28 0.56 595 210 232 22 122.28 0.56 596 211 232 21 121.86 0.34 597 213 218 5 856.84 0.85 598 214 218 4 856.84 0.84 599 214 219 5 811.92 4.63 600 214 222 8 332.81 30.16 601 214 231 17 122.67 0.54 602 214 233 19 122.56 0.56 603 214 236 22 122.11 0.64 604 214 245 31 53.09 2.05 605 215 218 3 884.43 3.37 606 215 219 4 852.36 1.39 607 215 236 21 122.11 0.64 608 215 241 26 61.43 0.93 609 215 245 30 53.19 2.10 610 216 219 3 883.77 3.70 611 216 220 4 883.22 3.98 612 216 241 25 61.41 0.93 613 216 244 28 56.65 0.37 614 216 245 29 53.11 2.06 615 217 220 3 933.46 3.44 616 217 241 24 63.95 2.19 617 218 221 3 865.98 3.73 618 218 222 4 850.74 3.69 619 219 222 3 851.55 3.29 620 219 223 4 850.65 3.73 621 219 231 12 122.80 0.55 622 219 245 26 49.26 0.73 623 219 248 29 34.34 0.05 624 220 223 3 851.93 3.50 625 220 224 4 851.09 3.51 626 220 248 28 33.77 0.24 627 221 224 3 852.58 3.18 628 222 225 3 882.58 5.20 629 222 228 6 271.20 20.86 630 222 231 9 212.41 45.36 631 223 228 5 271.20 20.87 632 223 229 6 266.32 18.43 633 223 230 7 212.52 45.32 634 223 231 8 212.50 45.32 635 223 248 25 33.75 0.24 636 223 249 26 31.75 0.42 637 223 250 27 33.11 0.33 638 225 228 3 871.21 6.32 639 228 231 3 1044.17 3.00 640 230 249 19 23.96 0.98 641 231 245 14 42.12 0.14 642 231 249 18 26.59 0.17 643 233 236 3 893.52 1.57 644 233 237 4 819.74 9.04 645 233 242 9 60.27 0.37 646 233 245 12 50.63 0.20 647 234 237 3 890.07 3.26 648 235 238 3 894.80 0.90 649 235 239 4 871.32 0.45 650 236 239 3 883.56 6.52 651 236 241 5 481.19 105.75 652 236 242 6 278.44 23.00 653 236 245 9 278.43 23.00 654 237 242 5 298.90 25.93 655 239 242 3 875.44 0.83 656 240 243 3 892.43 0.26 657 241 244 3 864.76 2.67 658 241 245 4 853.27 1.56 659 242 245 3 847.19 1.51 660 242 246 4 841.98 4.09 661 243 246 3 855.72 0.07 662 243 247 4 850.72 0.29 663 244 247 3 855.72 0.06 664 244 248 4 850.72 0.29 665 245 248 3 861.99 2.70 666 245 249 4 859.53 4.70 667 246 249 3 879.15 2.73 668 247 250 3 873.85 0.04