BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 113.88 0.43 2 3 228 225 113.78 0.45 3 3 229 226 111.55 0.40 4 4 204 200 167.70 0.83 5 4 205 201 163.57 1.16 6 4 206 202 154.80 3.21 7 4 207 203 153.76 3.12 8 4 229 225 114.74 0.10 9 5 36 31 466.24 0.21 10 5 37 32 466.22 0.20 11 5 38 33 466.19 0.20 12 5 207 202 164.58 0.77 13 6 36 30 466.31 0.18 14 6 37 31 466.22 0.20 15 6 38 32 466.22 0.20 16 6 223 217 115.09 0.01 17 6 229 223 115.07 0.01 18 6 249 243 24.38 0.78 19 7 37 30 466.29 0.20 20 7 38 31 466.24 0.20 21 7 40 33 466.24 0.20 22 7 90 83 191.06 1.39 23 7 163 156 184.97 0.99 24 7 207 200 180.62 1.08 25 7 209 202 167.16 0.99 26 7 229 222 115.39 0.12 27 7 230 223 115.07 0.01 28 7 231 224 114.98 0.04 29 8 37 29 466.38 0.16 30 8 38 30 466.31 0.20 31 8 39 31 466.25 0.19 32 8 40 32 466.25 0.19 33 8 231 223 115.08 0.00 34 8 242 234 61.48 0.24 35 8 246 238 41.24 0.64 36 9 40 31 466.25 0.19 37 9 124 115 188.77 1.32 38 9 231 222 115.36 0.10 39 9 232 223 115.05 0.00 40 9 233 224 114.92 0.02 41 9 242 233 61.54 0.26 42 10 40 30 466.38 0.17 43 10 41 31 466.38 0.16 44 10 42 32 466.44 0.14 45 10 237 227 90.00 0.55 46 10 242 232 61.54 0.26 47 11 42 31 466.49 0.12 48 11 65 54 465.47 0.18 49 11 95 84 189.44 1.15 50 11 232 221 116.52 0.26 51 11 233 222 115.14 0.01 52 11 234 223 115.01 0.02 53 11 237 226 94.28 0.78 54 12 15 3 892.74 0.63 55 12 21 9 470.29 1.45 56 12 24 12 467.42 0.01 57 12 25 13 467.39 0.00 58 12 28 16 467.39 0.01 59 12 41 29 466.54 0.12 60 12 42 30 466.53 0.12 61 12 43 31 466.53 0.12 62 12 51 39 466.62 0.14 63 12 65 53 466.12 0.19 64 12 237 225 114.12 0.04 65 12 238 226 91.98 0.38 66 13 44 31 466.59 0.12 67 13 65 52 466.11 0.20 68 13 97 84 189.38 1.11 69 13 234 221 116.34 0.35 70 14 44 30 466.62 0.13 71 15 44 29 466.64 0.12 72 15 47 32 466.85 0.14 73 15 238 223 114.77 0.05 74 16 20 4 849.62 5.78 75 16 21 5 830.94 0.65 76 16 47 31 466.85 0.14 77 17 20 3 863.50 1.16 78 17 21 4 846.74 7.21 79 18 21 3 864.59 1.71 80 18 22 4 849.64 5.76 81 18 50 32 467.88 0.10 82 19 22 3 864.56 1.69 83 19 23 4 856.22 2.48 84 20 23 3 864.27 1.54 85 20 24 4 863.07 0.96 86 21 24 3 856.20 2.47 87 21 25 4 856.19 2.48 88 21 47 26 468.87 0.27 89 21 50 29 470.67 0.54 90 21 238 217 112.06 0.16 91 22 25 3 854.81 1.79 92 22 26 4 852.97 2.23 93 22 50 28 470.63 0.52 94 22 54 32 470.75 0.56 95 23 26 3 856.05 0.98 96 23 27 4 852.99 2.25 97 24 27 3 853.77 1.85 98 24 28 4 849.35 0.93 99 24 237 213 112.17 0.11 100 24 238 214 111.45 0.06 101 24 239 215 90.08 0.54 102 24 240 216 90.00 0.54 103 25 28 3 852.81 1.37 104 25 29 4 850.58 0.26 105 25 50 25 470.99 0.53 106 25 51 26 470.84 0.53 107 25 54 29 471.05 0.50 108 25 55 30 470.93 0.56 109 26 29 3 852.93 1.31 110 26 30 4 839.77 3.85 111 26 54 28 471.45 0.30 112 27 30 3 857.46 3.58 113 27 31 4 845.31 1.08 114 27 240 213 81.12 0.92 115 27 243 216 63.02 0.36 116 28 31 3 851.11 2.21 117 28 41 13 471.18 0.49 118 28 55 27 471.66 0.37 119 28 57 29 471.65 0.36 120 28 60 32 471.60 0.36 121 28 237 209 111.94 0.19 122 28 242 214 65.44 0.85 123 28 243 215 61.54 0.25 124 29 54 25 471.66 0.37 125 29 55 26 471.65 0.38 126 29 56 27 471.65 0.38 127 29 57 28 471.57 0.41 128 31 57 26 472.44 0.72 129 31 243 212 61.17 0.09 130 31 247 216 51.10 0.76 131 32 247 215 51.08 0.76 132 33 37 4 872.48 5.49 133 33 57 24 546.69 0.68 134 33 59 26 524.82 2.29 135 33 60 27 524.82 2.29 136 33 246 213 51.04 0.77 137 33 247 214 50.66 0.59 138 34 59 25 564.50 1.03 139 35 59 24 569.14 0.38 140 35 246 211 51.02 0.76 141 35 249 214 36.39 0.99 142 35 250 215 26.98 0.53 143 37 59 22 569.27 0.36 144 37 246 209 44.62 0.59 145 37 249 212 31.98 1.46 146 38 59 21 569.29 0.38 147 38 60 22 569.25 0.38 148 38 61 23 569.22 0.36 149 38 90 52 191.14 1.35 150 39 59 20 569.35 0.35 151 39 60 21 569.25 0.38 152 39 61 22 569.25 0.38 153 39 246 207 41.34 0.68 154 40 61 21 569.25 0.38 155 40 63 23 569.19 0.36 156 40 90 50 191.15 1.35 157 41 51 10 569.50 0.35 158 41 55 14 569.50 0.35 159 41 60 19 569.46 0.34 160 41 61 20 569.39 0.34 161 41 62 21 569.22 0.37 162 41 63 22 569.20 0.36 163 42 63 21 569.21 0.36 164 42 65 23 569.00 0.45 165 42 92 50 191.99 1.47 166 43 47 4 829.94 0.60 167 43 48 5 570.18 0.60 168 43 63 20 569.43 0.35 169 43 65 22 569.20 0.36 170 44 47 3 863.39 6.50 171 44 48 4 849.13 0.58 172 44 65 21 569.38 0.33 173 45 48 3 863.76 6.74 174 45 64 19 569.45 0.35 175 45 66 21 569.39 0.34 176 45 78 33 210.24 3.17 177 45 83 38 208.08 3.95 178 47 50 3 872.98 0.36 179 47 51 4 832.98 4.47 180 48 51 3 868.58 1.83 181 48 52 4 833.88 4.04 182 48 62 14 569.58 0.35 183 48 63 15 569.57 0.35 184 48 64 16 569.50 0.37 185 48 83 35 205.21 4.11 186 48 86 38 195.72 1.12 187 48 88 40 195.05 0.95 188 49 52 3 857.53 4.70 189 49 53 4 857.49 4.69 190 49 86 37 195.18 0.85 191 50 53 3 858.11 4.41 192 50 54 4 857.50 4.69 193 51 54 3 860.38 3.28 194 51 55 4 854.43 6.23 195 51 62 11 569.65 0.30 196 52 55 3 873.38 0.67 197 52 62 10 569.67 0.31 198 52 86 34 195.12 0.87 199 55 60 5 569.75 0.29 200 55 62 7 569.71 0.29 201 57 60 3 873.51 6.67 202 60 242 182 62.96 0.38 203 60 246 186 50.75 0.76 204 61 90 29 191.72 1.65 205 62 88 26 192.91 1.81 206 62 90 28 191.76 1.66 207 63 88 25 193.47 1.72 208 63 90 27 191.95 1.56 209 63 91 28 192.83 1.77 210 64 83 19 203.24 5.05 211 64 88 24 194.87 1.53 212 64 90 26 193.31 1.78 213 64 91 27 192.98 1.79 214 64 92 28 193.22 1.86 215 65 92 27 193.22 1.86 216 66 91 25 195.00 1.50 217 66 92 26 194.38 1.79 218 66 93 27 194.38 1.79 219 66 98 32 194.86 1.56 220 66 104 38 195.97 1.58 221 66 112 46 196.41 1.79 222 67 78 11 210.26 3.16 223 67 79 12 210.11 3.17 224 67 80 13 210.18 3.14 225 67 83 16 209.69 3.37 226 67 91 24 195.39 1.45 227 67 93 26 195.29 1.34 228 67 112 45 196.56 1.86 229 68 74 6 211.16 3.35 230 68 77 9 210.53 3.06 231 68 78 10 210.53 3.07 232 68 79 11 210.27 3.10 233 68 80 12 210.37 3.08 234 68 112 44 196.39 1.79 235 69 80 11 210.44 3.10 236 69 81 12 210.53 3.07 237 69 115 46 199.13 1.38 238 70 74 4 919.31 4.29 239 70 79 9 210.53 3.06 240 70 81 11 210.55 3.08 241 70 115 45 199.86 1.08 242 71 79 8 210.56 3.06 243 71 81 10 210.56 3.07 244 71 82 11 210.56 3.06 245 74 77 3 848.02 0.60 246 78 82 4 847.70 1.56 247 78 83 5 839.27 2.64 248 79 82 3 864.39 3.54 249 79 83 4 844.36 0.10 250 80 83 3 863.77 3.22 251 80 84 4 839.32 2.66 252 80 91 11 324.64 5.16 253 80 115 35 326.20 5.92 254 80 116 36 280.26 17.05 255 80 121 41 480.70 1.41 256 81 84 3 853.00 4.19 257 81 85 4 845.39 3.25 258 82 85 3 868.71 4.12 259 82 86 4 845.40 3.25 260 83 86 3 848.60 2.23 261 83 87 4 842.88 4.51 262 83 88 5 632.04 20.33 263 83 91 8 440.16 19.50 264 83 121 38 580.99 5.19 265 84 87 3 845.98 3.54 266 84 119 35 581.24 5.07 267 84 121 37 581.09 5.15 268 88 121 33 663.62 0.09 269 89 120 31 663.68 0.11 270 89 121 32 663.61 0.09 271 89 122 33 663.57 0.10 272 90 121 31 663.63 0.09 273 90 122 32 663.57 0.10 274 90 124 34 663.55 0.10 275 90 163 73 663.58 0.11 276 91 116 25 663.78 0.10 277 91 121 30 663.76 0.10 278 91 122 31 663.71 0.09 279 91 123 32 663.59 0.10 280 91 124 33 663.58 0.11 281 92 124 32 663.62 0.09 282 92 126 34 663.56 0.10 283 92 232 140 120.32 0.70 284 93 112 19 663.81 0.12 285 93 113 20 663.79 0.10 286 93 116 23 663.89 0.07 287 93 123 30 663.75 0.10 288 93 124 31 663.72 0.10 289 93 125 32 663.59 0.10 290 93 126 33 663.57 0.10 291 94 99 5 819.45 9.15 292 94 104 10 663.81 0.10 293 94 109 15 663.79 0.11 294 94 126 32 663.62 0.09 295 95 98 3 850.77 2.49 296 95 99 4 843.10 0.80 297 95 126 31 663.67 0.09 298 95 167 72 663.54 0.12 299 95 232 137 121.29 0.59 300 96 99 3 853.51 0.19 301 96 100 4 836.95 3.87 302 96 167 71 663.55 0.12 303 96 169 73 661.27 0.27 304 97 100 3 856.06 1.47 305 97 101 4 834.08 5.30 306 97 102 5 830.62 3.60 307 98 102 4 819.47 9.17 308 98 103 5 819.36 9.12 309 98 104 6 663.94 0.07 310 99 104 5 663.86 0.10 311 99 106 7 663.97 0.04 312 99 109 10 664.03 0.04 313 99 126 27 663.91 0.07 314 99 127 28 663.89 0.06 315 99 146 47 663.96 0.04 316 103 108 5 856.30 0.48 317 104 108 4 844.79 6.24 318 104 109 5 845.19 5.06 319 104 112 8 823.41 0.61 320 105 108 3 880.13 1.78 321 105 109 4 864.82 3.77 322 106 109 3 871.52 6.08 323 106 110 4 864.83 3.78 324 106 145 39 664.19 0.10 325 106 146 40 664.07 0.06 326 106 149 43 664.22 0.12 327 107 110 3 864.84 3.79 328 107 111 4 847.27 4.05 329 108 111 3 856.36 8.02 330 108 112 4 847.28 4.05 331 109 112 3 857.43 1.02 332 109 113 4 855.78 0.20 333 109 150 41 664.35 0.09 334 110 113 3 858.40 0.53 335 110 114 4 858.40 0.53 336 110 149 39 664.35 0.09 337 110 150 40 664.34 0.09 338 110 153 43 664.74 0.10 339 111 114 3 871.95 6.24 340 111 115 4 857.28 1.09 341 111 153 42 664.88 0.09 342 112 115 3 868.89 1.81 343 112 116 4 840.80 1.24 344 113 116 3 868.88 1.81 345 113 117 4 857.86 7.29 346 113 123 10 664.58 0.15 347 113 125 12 664.33 0.08 348 113 150 37 664.61 0.17 349 113 154 41 664.99 0.05 350 114 117 3 878.44 2.98 351 114 118 4 857.86 7.29 352 114 153 39 664.97 0.05 353 114 154 40 664.98 0.06 354 115 118 3 878.44 2.98 355 115 119 4 865.57 3.46 356 116 119 3 880.01 2.19 357 116 120 4 856.03 8.23 358 116 121 5 794.97 2.79 359 116 123 7 665.01 0.04 360 117 120 3 873.53 1.04 361 117 123 6 665.03 0.03 362 117 154 37 693.14 8.96 363 117 158 41 695.37 7.84 364 117 161 44 670.98 0.44 365 117 162 45 667.60 1.24 366 120 161 41 784.85 1.69 367 121 161 40 784.86 1.69 368 122 160 38 784.91 1.70 369 122 161 39 784.86 1.69 370 122 207 85 298.56 1.84 371 123 161 38 784.91 1.70 372 123 162 39 784.88 1.69 373 123 163 40 784.85 1.68 374 124 163 39 784.86 1.68 375 124 165 41 784.81 1.70 376 124 209 85 298.48 1.86 377 125 150 25 785.05 1.74 378 125 163 38 784.90 1.69 379 125 164 39 784.87 1.69 380 125 165 40 784.86 1.69 381 126 165 39 784.87 1.69 382 126 167 41 784.84 1.68 383 126 232 106 121.06 0.65 384 127 146 19 785.24 1.74 385 127 147 20 785.20 1.76 386 127 150 23 785.21 1.76 387 127 164 37 784.94 1.69 388 127 165 38 784.90 1.69 389 127 166 39 784.88 1.69 390 127 167 40 784.86 1.68 391 127 190 63 302.94 1.84 392 128 143 15 785.43 1.75 393 128 146 18 785.44 1.76 394 128 166 38 784.89 1.69 395 128 167 39 784.87 1.69 396 128 169 41 784.40 1.78 397 129 133 4 827.41 0.96 398 129 134 5 827.39 0.97 399 129 139 10 785.56 1.72 400 129 142 13 785.49 1.75 401 129 143 14 785.47 1.76 402 129 166 37 784.94 1.70 403 129 167 38 784.89 1.69 404 129 169 40 784.51 1.81 405 129 170 41 784.26 1.84 406 130 133 3 842.40 6.61 407 130 134 4 835.72 4.22 408 130 169 39 784.51 1.81 409 130 170 40 784.37 1.78 410 131 134 3 851.46 2.04 411 131 135 4 848.36 2.10 412 131 170 39 784.32 1.73 413 131 174 43 745.57 3.37 414 131 176 45 680.14 5.09 415 132 135 3 865.55 1.57 416 132 136 4 849.97 1.29 417 133 136 3 881.35 0.94 418 133 137 4 857.49 2.46 419 133 138 5 785.95 1.54 420 133 142 9 785.62 1.70 421 134 137 3 867.90 7.66 422 134 139 5 785.64 1.71 423 134 166 32 784.96 1.69 424 134 170 36 784.38 1.75 425 138 141 3 849.92 3.55 426 138 142 4 835.68 5.82 427 139 142 3 865.28 0.67 428 139 143 4 848.70 0.70 429 139 189 50 303.42 1.77 430 140 143 3 861.80 3.26 431 140 144 4 844.89 2.60 432 140 189 49 303.38 1.77 433 141 145 4 862.29 0.10 434 142 145 3 862.30 0.11 435 142 146 4 859.44 1.32 436 143 146 3 870.34 3.92 437 143 147 4 864.76 1.80 438 143 166 23 784.93 1.68 439 143 189 46 303.17 1.80 440 143 190 47 303.07 1.82 441 144 147 3 873.26 2.45 442 144 148 4 855.59 3.45 443 144 189 45 303.23 1.80 444 144 193 49 302.83 1.89 445 145 148 3 858.53 4.92 446 145 149 4 861.81 3.27 447 146 149 3 865.27 1.55 448 146 150 4 863.40 2.48 449 147 150 3 863.41 2.48 450 147 151 4 854.44 2.01 451 147 159 12 785.52 1.61 452 147 164 17 784.95 1.69 453 147 193 46 302.63 1.79 454 148 151 3 861.65 1.61 455 148 152 4 847.34 1.54 456 148 193 45 302.64 1.80 457 149 152 3 872.58 3.54 458 150 153 3 879.20 0.24 459 150 154 4 864.48 0.94 460 151 154 3 870.34 3.88 461 151 159 8 785.75 1.66 462 151 193 42 302.62 1.79 463 151 198 47 300.02 1.97 464 154 158 4 837.43 8.64 465 154 159 5 824.91 3.95 466 154 162 8 785.69 1.69 467 155 158 3 869.97 3.75 468 155 159 4 859.18 2.69 469 156 159 3 876.80 0.34 470 156 198 42 300.00 1.97 471 156 201 45 299.87 1.94 472 156 202 46 299.41 2.01 473 157 160 3 888.05 3.16 474 158 161 3 879.89 1.22 475 158 162 4 858.27 2.64 476 159 162 3 849.00 0.98 477 159 194 35 301.74 1.63 478 159 198 39 300.00 1.97 479 159 202 43 299.38 2.00 480 159 206 47 298.72 1.88 481 162 206 44 298.70 1.89 482 163 207 44 298.57 1.84 483 163 209 46 298.49 1.86 484 164 190 26 302.63 1.77 485 164 206 42 298.87 1.91 486 164 207 43 298.61 1.85 487 164 208 44 298.49 1.86 488 164 209 45 298.49 1.86 489 165 209 44 298.48 1.86 490 165 232 67 121.04 0.65 491 166 186 20 303.05 1.83 492 166 187 21 302.98 1.84 493 166 190 24 302.91 1.85 494 166 208 42 298.62 1.84 495 166 209 43 298.52 1.85 496 166 210 44 298.47 1.86 497 166 211 45 298.38 1.84 498 167 211 44 298.10 1.84 499 168 171 3 869.96 3.97 500 168 172 4 857.53 1.34 501 168 210 42 298.54 1.83 502 168 211 43 298.45 1.86 503 168 212 44 298.09 1.84 504 169 172 3 859.50 0.29 505 170 174 4 818.98 7.71 506 170 176 6 495.73 4.44 507 170 178 8 311.56 1.89 508 170 182 12 304.12 1.55 509 171 174 3 862.40 4.45 510 171 176 5 344.26 2.02 511 171 178 7 304.34 1.66 512 172 212 40 297.93 1.61 513 177 182 5 853.10 3.08 514 178 182 4 843.59 1.66 515 178 210 32 298.83 1.82 516 179 182 3 860.00 0.37 517 179 183 4 850.86 1.97 518 180 183 3 857.63 5.08 519 180 184 4 846.69 0.12 520 180 221 41 239.15 5.63 521 180 222 42 224.63 0.85 522 180 225 45 128.72 2.69 523 181 184 3 861.13 0.43 524 181 185 4 846.72 0.10 525 182 185 3 849.92 1.69 526 182 186 4 846.84 0.17 527 183 186 3 862.66 1.20 528 183 187 4 847.90 0.69 529 183 210 27 298.92 1.85 530 183 222 39 224.61 0.84 531 184 187 3 847.91 0.69 532 184 188 4 847.90 0.69 533 184 225 41 125.76 1.93 534 184 226 42 125.76 1.94 535 184 227 43 125.64 2.00 536 185 188 3 863.68 1.71 537 185 189 4 850.15 5.05 538 186 189 3 861.70 0.72 539 186 190 4 850.20 5.03 540 187 190 3 861.69 0.72 541 187 191 4 847.30 3.57 542 187 208 21 299.28 1.98 543 187 209 22 299.25 2.00 544 187 210 23 299.01 1.92 545 187 227 40 125.20 1.80 546 187 228 41 123.30 0.88 547 188 191 3 850.59 1.93 548 188 192 4 839.07 0.52 549 188 227 39 125.28 1.82 550 189 192 3 854.46 0.01 551 189 193 4 848.98 3.53 552 190 193 3 854.59 0.73 553 190 194 4 855.13 0.45 554 190 208 18 299.25 2.00 555 191 194 3 859.39 1.68 556 191 195 4 837.32 7.13 557 191 203 12 300.00 1.99 558 191 206 15 299.53 1.95 559 191 208 17 299.29 1.99 560 191 227 36 125.17 1.81 561 191 229 38 125.09 1.78 562 192 195 3 847.07 3.58 563 192 196 4 837.31 7.13 564 193 196 3 858.24 3.33 565 193 197 4 831.85 9.86 566 193 198 5 831.85 9.86 567 194 197 3 850.60 0.78 568 194 198 4 811.11 0.51 569 194 199 5 803.53 2.61 570 194 202 8 350.05 9.51 571 194 203 9 318.37 1.57 572 194 206 12 299.52 1.95 573 195 199 4 648.70 80.02 574 195 200 5 368.64 0.24 575 195 203 8 342.14 13.46 576 198 202 4 847.84 0.58 577 199 202 3 851.69 2.00 578 199 203 4 846.27 0.21 579 200 203 3 854.67 3.49 580 200 204 4 846.27 0.21 581 201 204 3 862.25 0.29 582 201 205 4 860.70 0.48 583 202 205 3 862.25 0.29 584 202 206 4 860.69 0.48 585 203 206 3 864.54 1.44 586 206 229 23 123.05 0.82 587 207 229 22 123.03 0.82 588 208 227 19 123.17 0.82 589 208 228 20 123.14 0.81 590 208 229 21 123.03 0.82 591 209 229 20 122.67 0.64 592 209 230 21 122.24 0.57 593 209 231 22 121.91 0.73 594 209 232 23 121.47 0.52 595 210 222 12 125.19 1.82 596 210 228 18 123.12 0.80 597 210 230 20 122.65 0.67 598 210 231 21 122.25 0.57 599 210 232 22 121.91 0.73 600 211 232 21 121.89 0.72 601 213 218 5 836.97 2.07 602 214 218 4 814.72 3.91 603 214 219 5 434.37 30.59 604 214 222 8 364.49 4.44 605 214 231 17 122.70 0.71 606 214 233 19 122.59 0.73 607 214 236 22 122.22 0.58 608 214 245 31 59.88 0.52 609 215 218 3 878.35 3.08 610 215 219 4 814.71 3.90 611 215 236 21 122.33 0.64 612 215 241 26 61.62 0.24 613 215 245 30 60.99 0.12 614 216 220 4 866.81 1.59 615 216 241 25 61.62 0.24 616 216 244 28 61.16 0.08 617 216 245 29 60.77 0.09 618 217 241 24 62.87 0.39 619 217 244 27 61.23 0.11 620 218 221 3 850.80 0.31 621 218 222 4 838.30 2.44 622 219 222 3 853.28 0.81 623 219 223 4 838.30 2.44 624 219 231 12 125.84 1.68 625 219 245 26 51.26 0.46 626 219 248 29 39.25 1.99 627 220 223 3 860.64 3.50 628 220 224 4 839.77 2.15 629 220 248 28 39.09 1.81 630 221 224 3 868.93 0.48 631 221 225 4 857.38 6.57 632 222 225 3 868.14 1.18 633 222 228 6 330.82 0.90 634 222 231 9 222.75 45.37 635 223 228 5 323.25 4.69 636 223 229 6 323.25 4.69 637 223 230 7 222.98 45.41 638 223 231 8 222.98 45.41 639 223 248 25 38.88 1.80 640 223 249 26 31.50 0.03 641 223 250 27 31.73 0.14 642 225 228 3 852.74 3.65 643 228 231 3 1049.15 1.93 644 230 249 19 24.36 0.78 645 231 245 14 49.78 0.21 646 231 249 18 31.66 1.07 647 233 236 3 899.49 4.55 648 233 237 4 853.16 1.65 649 233 242 9 178.02 23.78 650 233 245 12 51.38 0.52 651 234 237 3 878.58 2.48 652 234 238 4 869.65 1.69 653 235 238 3 884.55 3.93 654 235 239 4 869.25 1.49 655 236 239 3 865.70 2.79 656 236 241 5 636.74 26.04 657 236 242 6 320.01 3.93 658 236 245 9 319.99 3.93 659 237 242 5 352.55 1.43 660 239 242 3 870.76 1.51 661 240 243 3 884.32 3.80 662 241 244 3 864.88 3.27 663 241 245 4 852.14 3.18 664 242 245 3 852.14 3.18 665 242 246 4 831.11 7.50 666 243 246 3 854.11 2.19 667 243 247 4 842.59 1.51 668 244 247 3 847.58 4.00 669 244 248 4 842.58 1.51 670 245 248 3 850.04 0.04 671 245 249 4 850.73 0.48 672 246 249 3 883.04 1.05 673 247 250 3 883.44 1.25