BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 8 12 4 851.14 0.10 2 8 127 120 45.47 1.02 3 8 128 121 30.75 1.38 4 9 127 119 45.73 1.03 5 10 127 118 46.49 1.23 6 10 128 119 46.52 1.25 7 11 14 3 864.66 4.41 8 11 125 115 46.53 1.26 9 11 127 117 46.55 1.25 10 12 23 11 46.55 1.25 11 12 128 117 46.59 1.23 12 12 129 118 46.57 1.24 13 13 21 8 46.59 1.24 14 13 23 10 46.55 1.25 15 13 24 11 46.58 1.24 16 13 124 112 46.57 1.25 17 13 131 119 46.58 1.24 18 14 21 7 46.68 1.27 19 15 20 5 534.19 42.45 20 15 21 6 534.06 42.40 21 16 19 3 790.95 6.30 22 16 20 4 621.10 0.99 23 20 25 5 801.48 5.87 24 21 24 3 834.61 0.42 25 21 25 4 824.37 5.53 26 22 25 3 834.61 0.42 27 22 26 4 824.37 5.53 28 23 26 3 827.84 3.80 29 23 27 4 808.69 2.33 30 23 131 109 367.91 0.76 31 24 27 3 821.72 0.74 32 24 28 4 808.59 2.39 33 24 124 101 367.88 0.76 34 24 131 108 369.68 0.12 35 24 153 131 367.82 0.79 36 24 154 132 366.99 0.36 37 25 28 3 828.44 3.17 38 25 29 4 808.59 2.39 39 26 29 3 825.40 6.02 40 26 30 4 815.49 1.06 41 27 30 3 815.88 1.26 42 27 31 4 815.49 1.06 43 27 131 105 370.12 0.33 44 27 132 106 370.12 0.34 45 27 133 107 370.11 0.34 46 27 153 128 369.95 0.28 47 28 31 3 826.74 7.63 48 28 32 4 814.52 1.54 49 28 153 127 370.07 0.34 50 29 32 3 820.58 4.55 51 29 33 4 809.31 1.96 52 30 33 3 824.72 3.58 53 30 34 4 804.58 0.41 54 30 80 50 371.04 0.23 55 31 34 3 811.48 3.04 56 31 79 48 370.70 0.40 57 31 80 49 370.70 0.40 58 31 133 103 370.69 0.41 59 31 134 104 370.66 0.41 60 31 135 105 370.61 0.39 61 31 153 124 370.66 0.41 62 31 166 137 340.25 0.84 63 32 166 136 340.25 0.84 64 32 167 137 340.22 0.84 65 32 169 139 339.80 1.03 66 32 170 140 339.80 1.03 67 33 167 136 340.24 0.82 68 34 80 46 371.16 0.24 69 34 166 134 340.39 0.90 70 34 167 135 340.27 0.83 71 35 51 16 429.82 1.48 72 35 53 18 429.72 1.54 73 35 66 31 429.64 1.50 74 35 79 44 388.71 0.43 75 36 50 14 429.82 1.48 76 36 51 15 429.84 1.48 77 36 79 43 403.75 7.69 78 36 165 131 341.23 1.03 79 36 166 132 340.66 1.01 80 36 167 133 340.66 1.01 81 36 168 134 340.63 1.01 82 36 197 163 257.57 1.44 83 36 200 166 257.03 1.32 84 37 49 12 429.86 1.48 85 37 50 13 429.82 1.47 86 37 77 40 429.62 1.50 87 37 78 41 426.60 1.45 88 37 79 42 406.41 8.63 89 37 137 101 428.12 0.82 90 37 164 129 341.29 1.03 91 37 165 130 341.26 1.04 92 38 48 10 429.86 1.48 93 38 49 11 429.87 1.49 94 38 137 100 429.64 1.49 95 38 163 127 341.63 1.13 96 38 164 128 341.29 1.03 97 38 193 157 260.16 0.84 98 38 196 160 260.13 0.85 99 38 197 161 259.89 0.85 100 39 47 8 429.92 1.49 101 39 48 9 429.87 1.48 102 39 137 99 429.81 1.47 103 39 148 111 429.81 1.47 104 39 162 125 364.24 2.77 105 39 163 126 345.67 3.01 106 40 46 6 436.75 4.88 107 40 47 7 429.99 1.52 108 40 148 110 429.89 1.46 109 40 161 123 369.90 0.56 110 40 162 124 369.12 0.37 111 40 176 138 326.44 0.47 112 40 180 142 310.50 1.84 113 40 184 146 278.82 3.71 114 40 189 151 260.32 0.88 115 40 192 154 260.32 0.87 116 40 193 155 260.24 0.87 117 41 45 4 725.95 5.63 118 41 46 5 436.75 4.88 119 41 147 107 430.12 1.55 120 41 14 108 430.07 1.53 121 41 148 109 429.95 1.49 122 41 160 121 370.90 0.41 123 41 161 122 370.16 0.43 124 41 180 141 314.00 0.10 125 42 45 3 805.31 4.02 126 42 184 144 274.05 1.35 127 42 185 145 273.99 1.33 128 42 186 146 266.93 2.14 129 42 189 149 261.42 0.69 130 43 182 141 309.98 1.66 131 43 183 142 294.67 4.51 132 43 184 143 286.95 0.76 133 44 147 104 443.34 1.09 134 44 185 143 274.13 1.34 135 44 186 144 274.01 1.35 136 44 216 174 256.18 1.28 137 45 147 103 443.38 1.07 138 45 186 143 266.83 2.17 139 45 215 172 256.19 1.29 140 45 216 173 256.03 1.27 141 46 139 94 443.38 1.06 142 46 147 102 443.41 1.06 143 46 14 103 443.41 1.06 144 46 148 104 443.33 1.08 145 46 186 142 266.76 2.21 146 46 214 170 256.22 1.29 147 46 215 171 256.07 1.26 148 47 186 141 266.76 2.21 149 47 189 144 260.49 0.83 150 47 190 145 260.37 0.86 151 47 193 148 260.34 0.87 152 47 212 167 256.42 1.26 153 47 213 168 256.30 1.27 154 47 214 169 256.06 1.26 155 48 67 19 443.69 1.11 156 48 75 27 443.70 1.11 157 48 76 28 443.68 1.10 158 48 77 29 443.60 1.11 159 48 137 90 443.60 1.11 160 48 139 92 443.68 1.10 161 48 148 102 443.65 1.10 162 48 212 166 256.31 1.27 163 48 213 167 256.21 1.28 164 49 197 150 260.12 0.86 165 49 211 164 256.36 1.25 166 49 212 165 256.11 1.23 167 50 66 16 443.71 1.12 168 50 67 17 443.72 1.11 169 50 77 27 443.71 1.11 170 50 197 149 260.09 0.87 171 50 210 162 256.38 1.26 172 50 211 163 256.20 1.23 173 51 66 15 443.79 1.10 174 51 202 152 257.22 1.28 175 51 20 160 256.34 1.24 176 52 61 9 444.16 1.04 177 52 66 14 443.84 1.12 178 52 209 158 256.35 1.23 179 52 20 159 256.31 1.23 180 52 210 160 256.19 1.21 181 52 211 161 256.18 1.21 182 54 59 5 621.88 2.62 183 54 61 7 444.18 1.04 184 56 59 3 800.95 7.81 185 60 63 3 829.72 3.21 186 61 64 3 819.25 6.11 187 65 211 148 256.36 1.26 188 65 223 175 120.87 0.27 189 66 76 10 575.94 20.39 190 66 77 11 564.73 25.96 191 66 78 12 564.61 26.01 192 66 85 19 563.58 25.69 193 66 211 147 256.34 1.25 194 67 76 9 575.90 20.38 195 67 77 10 573.63 21.51 196 67 85 18 573.59 21.41 197 67 211 146 256.38 1.25 198 67 213 148 256.22 1.29 199 67 221 171 127.28 0.42 200 67 223 173 114.80 2.77 201 68 75 7 617.03 0.07 202 68 76 8 578.06 19.36 203 68 85 17 577.87 19.27 204 68 89 21 575.07 19.94 205 68 93 25 577.60 18.68 206 69 72 3 865.31 1.55 207 69 74 5 627.48 4.86 208 69 75 6 617.29 0.06 209 69 93 24 577.60 18.68 210 69 221 169 127.19 0.47 211 70 74 4 700.00 16.73 212 70 76 6 618.67 0.48 213 70 89 19 578.10 18.96 214 70 92 22 577.64 18.71 215 70 93 23 577.59 18.67 216 70 112 43 612.83 0.32 217 70 138 69 547.36 3.55 218 71 74 3 807.56 1.63 219 71 96 25 614.01 0.47 220 71 106 36 613.79 0.70 221 71 107 37 613.25 0.42 222 71 112 42 613.03 0.39 223 72 106 35 614.11 0.82 224 72 219 164 127.30 0.41 225 72 220 165 127.33 0.42 226 72 221 166 127.17 0.48 227 73 140 68 605.16 1.09 228 73 141 69 605.15 1.10 229 73 215 144 256.25 1.30 230 73 219 163 127.36 0.43 231 73 220 164 127.31 0.42 232 73 221 165 126.98 0.58 233 74 107 34 614.12 0.88 234 74 108 35 614.09 0.88 235 74 109 36 613.99 0.86 236 74 112 39 613.98 0.87 237 74 138 65 605.46 1.10 238 74 139 66 605.37 1.08 239 74 140 67 605.16 1.09 240 74 215 143 256.18 1.29 241 75 138 64 605.48 1.10 242 75 139 65 605.37 1.08 243 75 215 142 256.16 1.28 244 75 221 163 127.18 0.47 245 76 85 9 616.86 0.06 246 76 137 62 605.69 1.09 247 76 138 63 605.48 1.10 248 77 85 8 616.86 0.06 249 77 136 60 605.91 1.02 250 77 137 61 605.70 1.09 251 78 82 4 852.45 0.62 252 78 85 7 616.87 0.06 253 78 134 57 609.46 1.35 254 78 135 58 607.13 0.59 255 78 136 59 605.93 1.02 256 78 165 89 341.21 1.03 257 79 134 56 609.62 1.30 258 79 135 57 607.67 0.47 259 79 165 88 340.64 1.01 260 79 166 89 340.37 0.90 261 80 133 54 610.32 1.60 262 80 134 55 609.63 1.30 263 81 84 3 832.03 1.23 264 81 85 4 811.27 4.66 265 81 130 50 611.12 1.73 266 81 133 53 610.35 1.59 267 81 134 54 610.32 1.60 268 82 85 3 823.81 2.89 269 82 86 4 819.34 0.64 270 83 86 3 819.34 0.63 271 83 87 4 819.34 0.63 272 84 87 3 844.75 1.22 273 84 88 4 819.33 0.63 274 84 130 47 610.91 1.84 275 84 132 49 610.53 1.68 276 84 133 50 610.45 1.64 277 84 134 51 610.37 1.63 278 85 88 3 815.60 1.25 279 85 89 4 815.52 1.27 280 85 134 50 610.10 1.67 281 85 136 52 607.52 0.43 282 86 89 3 814.86 0.93 283 86 90 4 814.85 0.93 284 87 90 3 818.49 1.81 285 87 91 4 808.66 0.84 286 87 132 46 610.70 1.75 287 88 91 3 822.84 0.36 288 88 92 4 810.65 1.85 289 88 119 32 612.31 1.91 290 88 120 33 612.31 1.91 291 88 132 45 610.54 1.71 292 88 134 47 610.31 1.63 293 88 136 49 606.40 0.92 294 89 92 3 815.39 0.51 295 89 93 4 814.17 0.10 296 90 93 3 816.97 1.30 297 90 94 4 814.17 0.10 298 91 94 3 828.94 2.75 299 91 95 4 814.17 0.10 300 91 119 29 612.63 1.77 301 92 95 3 832.72 3.19 302 92 96 4 814.19 0.12 303 92 112 21 614.28 1.02 304 92 116 25 612.63 1.77 305 93 96 3 822.07 0.35 306 93 97 4 817.89 1.74 307 94 97 3 819.76 0.80 308 94 98 4 817.88 1.73 309 95 98 3 830.05 4.35 310 95 99 4 803.32 5.54 311 95 112 18 614.39 1.06 312 95 115 21 614.03 1.12 313 96 100 4 796.62 12.36 314 96 101 5 762.75 4.58 315 96 107 12 614.60 1.10 316 96 112 17 614.38 1.07 317 97 100 3 830.05 4.36 318 97 101 4 758.96 6.49 319 97 102 5 758.95 6.49 320 97 106 10 615.12 1.16 321 98 102 4 758.97 6.48 322 98 10 5 771.40 0.29 323 99 10 4 795.78 3.46 324 100 10 3 822.96 5.83 325 100 103 4 771.40 0.29 326 100 105 6 696.92 4.93 327 100 106 7 615.61 1.33 328 100 107 8 615.09 1.17 329 100 108 9 615.37 1.29 330 100 111 12 614.82 1.20 331 101 104 4 699.36 3.70 332 101 105 5 634.21 10.64 333 101 106 6 615.40 1.27 334 107 112 5 759.30 10.30 335 108 111 3 836.96 0.70 336 108 112 4 812.98 0.46 337 108 142 34 605.00 1.07 338 108 145 37 605.03 1.11 339 109 112 3 826.54 4.33 340 109 113 4 809.18 1.44 341 109 138 29 605.56 1.12 342 109 140 31 605.39 1.10 343 109 141 32 605.38 1.10 344 109 142 33 605.34 1.11 345 109 14 39 605.02 1.11 346 109 149 41 604.96 1.11 347 110 113 3 820.31 0.62 348 110 114 4 809.16 1.44 349 110 145 35 605.31 1.12 350 110 149 40 604.96 1.14 351 111 114 3 815.75 1.15 352 111 115 4 809.15 1.44 353 112 115 3 816.25 1.41 354 112 116 4 816.22 1.40 355 113 116 3 816.23 1.40 356 113 117 4 816.23 1.40 357 113 138 25 605.70 1.08 358 113 149 37 605.00 1.12 359 113 151 39 604.82 1.14 360 113 157 45 516.62 3.52 361 114 117 3 816.22 1.40 362 114 118 4 816.22 1.40 363 114 157 44 517.01 3.37 364 115 118 3 823.08 1.09 365 115 119 4 811.71 4.62 366 116 119 3 822.73 2.31 367 116 120 4 817.51 1.71 368 116 136 20 606.29 0.86 369 116 138 22 605.94 1.03 370 117 120 3 819.80 0.57 371 117 121 4 817.50 1.72 372 117 151 35 604.95 1.14 373 117 155 39 526.42 1.22 374 117 157 41 517.01 3.36 375 118 121 3 821.25 0.15 376 118 122 4 819.78 0.58 377 119 122 3 830.70 1.79 378 119 123 4 819.79 0.58 379 119 132 13 610.88 1.77 380 120 123 3 827.60 3.33 381 120 124 4 818.87 0.12 382 120 132 12 610.69 1.76 383 120 134 14 610.53 1.69 384 120 136 16 609.82 1.59 385 120 151 32 605.03 1.11 386 120 152 33 605.01 1.12 387 120 153 34 604.74 1.17 388 120 155 36 558.44 4.75 389 121 124 3 823.76 2.33 390 121 125 4 818.86 0.12 391 121 154 34 604.70 1.19 392 121 155 35 591.61 2.73 393 122 125 3 820.12 0.77 394 122 126 4 797.50 0.15 395 123 129 6 615.35 3.80 396 123 130 7 611.18 1.73 397 123 131 8 611.11 1.71 398 123 132 9 611.11 1.70 399 124 131 7 611.10 1.70 400 124 153 30 605.03 1.12 401 126 129 3 865.46 1.91 402 131 153 23 604.98 1.12 403 132 153 22 604.88 1.14 404 134 153 20 604.79 1.14 405 135 151 17 604.95 1.14 406 135 152 18 604.79 1.13 407 135 153 19 604.73 1.17 408 135 165 31 340.64 1.02 409 136 150 15 604.95 1.13 410 136 151 16 604.83 1.12 411 137 148 12 605.02 1.12 412 137 149 13 604.95 1.13 413 137 150 14 604.82 1.13 414 137 163 27 341.62 1.13 415 137 165 29 341.26 1.04 416 138 148 11 604.98 1.12 417 138 149 12 604.94 1.13 418 138 151 14 604.83 1.12 419 139 14 9 604.99 1.12 420 139 148 10 604.87 1.16 421 139 215 77 256.14 1.28 422 140 147 7 604.99 1.12 423 140 14 8 604.93 1.14 424 140 215 76 256.18 1.29 425 141 146 5 605.00 1.11 426 141 147 6 604.99 1.12 427 141 215 75 256.23 1.31 428 141 220 95 127.36 0.43 429 142 145 3 816.26 0.40 430 142 146 4 632.79 1.33 431 147 215 69 256.23 1.31 432 147 216 70 256.19 1.29 433 148 159 11 483.24 5.34 434 148 160 12 417.58 22.91 435 148 162 14 414.39 21.45 436 149 157 8 497.66 2.28 437 149 158 9 496.99 2.03 438 149 159 10 474.67 1.06 439 150 157 7 497.65 2.27 440 150 158 8 497.21 2.11 441 150 160 10 484.00 5.36 442 150 163 13 341.44 1.13 443 150 165 15 341.20 1.04 444 151 156 5 570.22 30.30 445 151 157 6 508.37 7.65 446 152 155 3 809.81 5.79 447 152 156 4 596.64 33.06 448 152 158 6 579.13 33.62 449 160 163 3 862.25 1.08 450 161 180 19 310.04 1.62 451 162 176 14 326.18 0.42 452 162 180 18 310.04 1.62 453 163 173 10 328.36 0.12 454 163 176 13 326.08 0.43 455 164 169 5 480.94 71.67 456 164 172 8 329.50 0.33 457 164 173 9 328.62 0.67 458 164 176 12 326.13 0.41 459 165 169 4 476.98 73.66 460 166 169 3 815.32 3.52 461 168 171 3 825.01 2.42 462 168 172 4 815.13 2.54 463 169 172 3 816.16 2.02 464 169 173 4 815.17 2.51 465 170 173 3 822.62 0.25 466 170 174 4 815.23 2.48 467 171 174 3 822.33 1.06 468 171 175 4 815.24 2.48 469 172 175 3 822.28 1.08 470 172 176 4 815.20 2.46 471 172 196 24 258.00 1.39 472 173 176 3 825.40 0.47 473 173 177 4 815.69 2.71 474 174 177 3 824.75 0.79 475 174 178 4 815.65 2.73 476 175 178 3 830.38 2.69 477 175 179 4 812.92 3.56 478 175 195 20 260.03 0.90 479 175 196 21 258.51 1.65 480 176 179 3 823.09 0.96 481 176 180 4 796.02 0.49 482 176 192 16 260.17 0.85 483 176 196 20 259.50 1.15 484 179 192 13 260.20 0.88 485 179 195 16 260.10 0.93 486 180 184 4 810.97 5.20 487 181 184 3 846.82 2.89 488 184 188 4 789.00 2.41 489 184 192 8 742.51 1.70 490 185 188 3 811.84 0.89 491 185 189 4 804.09 2.99 492 186 189 3 813.19 1.55 493 186 190 4 804.09 2.98 494 186 214 28 256.41 1.26 495 186 216 30 256.06 1.26 496 187 190 3 817.42 3.68 497 187 191 4 812.82 1.38 498 187 232 60 88.98 0.81 499 188 191 3 814.75 5.01 500 188 192 4 814.05 5.37 501 189 192 3 822.68 1.05 502 189 193 4 808.51 3.53 503 190 193 3 820.61 2.08 504 190 194 4 810.78 2.42 505 190 212 22 256.44 1.26 506 190 214 24 256.27 1.26 507 190 224 49 123.22 0.10 508 190 232 57 87.19 1.66 509 191 194 3 816.02 0.21 510 191 195 4 808.60 3.56 511 191 235 59 79.71 1.62 512 191 236 60 57.37 0.54 513 192 195 3 817.70 0.98 514 192 196 4 816.28 0.17 515 193 196 3 822.19 1.27 516 193 197 4 821.52 2.77 517 193 212 19 256.38 1.25 518 194 197 3 821.58 2.75 519 194 198 4 821.54 2.77 520 194 206 11 256.88 1.26 521 194 210 16 256.44 1.25 522 194 212 18 256.33 1.24 523 194 229 50 92.34 0.70 524 194 232 53 86.35 1.24 525 194 233 54 84.57 0.65 526 194 236 57 56.20 0.86 527 195 198 3 827.12 0.01 528 195 199 4 823.66 1.72 529 195 236 56 56.20 0.86 530 196 199 3 835.13 0.79 531 197 200 3 828.34 0.65 532 197 202 4 805.35 2.15 533 197 206 8 256.50 1.22 534 197 210 13 256.38 1.26 535 198 202 3 821.54 3.28 536 198 206 7 256.42 1.24 537 198 236 53 56.20 0.86 538 202 205 3 916.30 0.83 539 202 206 4 804.99 6.06 540 205 20 5 638.21 3.36 541 206 20 4 725.39 12.72 542 206 210 5 638.24 3.38 543 206 233 43 85.06 0.57 544 207 20 3 823.62 0.95 545 207 210 4 728.61 7.53 546 207 230 39 89.14 0.90 547 207 233 42 86.34 1.22 548 208 226 34 93.21 1.12 549 208 229 37 89.14 0.90 550 208 230 38 89.14 0.90 551 208 233 41 87.06 0.89 552 209 226 33 93.24 1.11 553 210 229 34 92.33 0.70 554 211 223 27 120.09 0.20 555 212 223 26 114.83 2.77 556 212 224 27 114.75 2.79 557 212 229 32 92.67 0.55 558 213 221 23 127.29 0.42 559 213 222 24 126.75 0.60 560 213 223 25 114.81 2.77 561 213 224 26 114.81 2.77 562 214 220 21 127.50 0.50 563 214 221 22 127.28 0.42 564 214 222 23 126.95 0.58 565 215 220 20 127.31 0.43 566 215 221 21 127.26 0.43 567 216 220 19 127.37 0.44 568 217 220 18 127.55 0.50 569 21 21 14 128.25 0.44 570 21 218 15 128.19 0.42 571 21 219 16 128.09 0.38 572 21 220 17 127.56 0.51 573 21 21 3 813.48 5.86 574 21 21 4 798.24 1.19 575 21 21 13 128.40 0.44 576 21 218 14 128.21 0.42 577 21 219 15 128.20 0.42 578 21 222 18 127.93 0.35 579 21 21 3 798.24 1.19 580 21 21 12 128.40 0.44 581 21 218 13 128.33 0.42 582 21 219 12 128.40 0.43 583 21 222 15 128.21 0.47 584 21 21 8 128.60 0.42 585 21 21 9 128.59 0.41 586 21 218 10 128.41 0.43 587 21 219 11 128.41 0.43 588 21 21 6 421.35 143.28 589 21 21 3 822.26 3.95 590 21 21 3 877.60 0.15 591 222 228 6 805.41 1.83 592 224 228 4 788.66 6.54 593 224 229 5 787.72 6.06 594 224 232 8 782.27 3.34 595 225 228 3 812.24 0.21 596 225 229 4 805.43 1.84 597 226 229 3 812.59 2.10 598 226 230 4 808.76 0.18 599 227 230 3 820.43 1.93 600 227 231 4 809.16 0.19 601 228 231 3 827.11 5.26 602 228 232 4 809.19 0.19 603 229 232 3 814.15 1.22 604 229 233 4 809.18 0.19 605 230 233 3 812.51 0.24 606 230 234 4 809.18 0.19 607 231 234 3 833.45 1.79 608 232 235 3 829.23 3.90 609 232 236 4 802.84 5.05 610 233 236 3 806.58 3.19