BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 8 127 120 48.06 0.95 2 8 128 121 29.39 0.74 3 9 127 119 51.12 1.72 4 10 127 118 52.43 1.71 5 10 128 119 52.26 1.62 6 11 14 3 864.96 3.77 7 11 125 115 52.44 1.69 8 11 126 116 52.47 1.71 9 11 127 117 52.46 1.71 10 12 23 11 52.48 1.71 11 12 128 117 52.47 1.71 12 12 129 118 52.46 1.71 13 13 21 8 52.52 1.72 14 13 23 10 52.48 1.71 15 13 24 11 52.47 1.71 16 13 124 112 52.48 1.70 17 13 131 119 52.47 1.71 18 14 21 7 52.59 1.69 19 15 20 5 252.73 98.28 20 15 21 6 52.61 1.69 21 16 19 3 811.19 4.32 22 16 20 4 568.73 63.48 23 20 25 5 779.50 1.72 24 21 24 3 834.10 0.16 25 21 25 4 794.68 9.30 26 22 25 3 823.87 5.29 27 22 26 4 795.07 9.11 28 23 26 3 823.85 5.29 29 23 27 4 808.27 2.49 30 23 131 109 374.53 1.43 31 24 27 3 812.83 3.71 32 24 28 4 808.29 2.48 33 24 124 101 374.55 1.42 34 24 131 108 376.73 0.34 35 24 153 131 374.24 1.51 36 24 154 132 374.12 1.54 37 25 28 3 826.99 3.39 38 25 29 4 808.31 2.47 39 26 29 3 824.84 5.79 40 26 30 4 814.92 0.84 41 27 30 3 834.15 1.14 42 27 31 4 807.41 2.02 43 27 131 105 380.36 1.06 44 27 132 106 380.36 1.06 45 27 133 107 380.36 1.06 46 27 153 128 380.30 1.03 47 28 31 3 826.24 7.38 48 28 32 4 811.85 0.19 49 28 152 126 380.35 1.05 50 28 153 127 380.36 1.06 51 29 32 3 820.09 4.30 52 29 33 4 811.85 0.19 53 30 33 3 825.28 3.29 54 30 34 4 808.68 1.78 55 30 80 50 380.69 1.01 56 31 34 3 810.97 2.79 57 31 79 48 380.68 1.00 58 31 80 49 380.68 1.01 59 31 133 103 380.68 1.01 60 31 134 104 380.66 1.00 61 31 135 105 380.62 1.02 62 31 153 124 380.61 0.99 63 31 166 137 339.90 0.69 64 32 166 136 339.92 0.68 65 32 167 137 339.88 0.69 66 32 169 139 339.16 0.63 67 32 170 140 338.25 0.25 68 33 167 136 339.89 0.69 69 34 80 46 381.33 0.71 70 34 166 134 340.07 0.73 71 34 167 135 339.94 0.69 72 35 51 16 419.48 3.19 73 35 53 18 419.29 3.21 74 35 66 31 418.57 2.82 75 35 79 44 388.22 0.18 76 35 167 134 340.08 0.73 77 36 50 14 419.51 3.19 78 36 51 15 419.51 3.19 79 36 164 130 340.83 0.81 80 36 165 131 340.76 0.81 81 36 166 132 340.20 0.72 82 36 167 133 340.13 0.73 83 36 168 134 340.10 0.74 84 36 197 163 258.58 1.43 85 36 200 166 257.78 1.55 86 37 49 12 419.56 3.18 87 37 50 13 419.51 3.19 88 37 77 40 417.27 3.25 89 37 78 41 413.63 2.26 90 37 79 42 411.81 3.17 91 37 137 101 417.44 4.17 92 37 164 129 340.84 0.81 93 37 165 130 340.79 0.82 94 38 48 10 419.62 3.20 95 38 49 11 419.63 3.20 96 38 137 100 418.98 3.40 97 38 163 127 341.49 0.81 98 38 164 128 340.84 0.81 99 38 193 157 260.16 0.86 100 38 196 160 260.13 0.86 101 38 197 161 259.99 0.90 102 39 47 8 436.25 5.08 103 39 48 9 419.63 3.20 104 39 137 99 419.37 3.25 105 39 148 111 419.51 3.18 106 39 162 125 368.55 0.58 107 39 163 126 345.27 2.70 108 40 46 6 525.07 49.45 109 40 47 7 436.33 5.08 110 40 148 110 419.72 3.18 111 40 161 123 379.64 0.84 112 40 162 124 368.62 0.60 113 40 176 138 332.19 1.34 114 40 180 142 308.31 1.26 115 40 184 146 274.44 0.99 116 40 189 151 260.54 0.76 117 40 192 154 260.50 0.78 118 40 193 155 260.29 0.86 119 41 45 4 758.49 5.53 120 41 46 5 436.35 5.09 121 41 147 107 419.84 3.17 122 41 14 108 419.82 3.18 123 41 148 109 419.75 3.18 124 41 160 121 381.29 0.84 125 41 161 122 380.36 0.49 126 41 180 141 312.91 0.06 127 42 45 3 804.81 3.77 128 42 184 144 273.25 0.67 129 42 185 145 271.16 1.68 130 42 186 146 265.08 1.15 131 42 189 149 262.80 0.07 132 43 182 141 308.18 1.25 133 43 183 142 295.32 4.18 134 43 184 143 286.43 0.99 135 44 147 104 442.47 1.53 136 44 185 143 273.85 1.29 137 44 186 144 265.39 1.20 138 45 147 103 442.48 1.53 139 45 186 143 264.34 0.77 140 45 215 172 257.14 1.69 141 45 216 173 256.93 1.68 142 46 139 94 442.51 1.53 143 46 147 102 442.50 1.54 144 46 14 103 442.49 1.53 145 46 148 104 442.50 1.53 146 46 186 142 264.29 0.74 147 46 214 170 257.14 1.69 148 46 215 171 256.96 1.68 149 47 186 141 264.27 0.73 150 47 189 144 260.62 0.77 151 47 190 145 260.57 0.74 152 47 193 148 260.49 0.78 153 47 212 167 257.41 1.60 154 47 213 168 257.19 1.67 155 47 214 169 256.94 1.67 156 48 67 19 442.88 1.51 157 48 75 27 442.87 1.51 158 48 76 28 442.86 1.51 159 48 77 29 442.82 1.50 160 48 137 90 442.72 1.55 161 48 139 92 442.74 1.55 162 48 148 102 442.73 1.55 163 48 212 166 257.19 1.67 164 48 213 167 257.11 1.67 165 49 197 150 260.15 0.86 166 49 211 164 257.36 1.60 167 49 212 165 257.01 1.66 168 50 66 16 442.98 1.48 169 50 67 17 443.00 1.48 170 50 77 27 442.94 1.49 171 50 197 149 260.07 0.84 172 50 210 162 257.36 1.60 173 50 211 163 257.13 1.64 174 51 66 15 443.06 1.46 175 51 197 148 260.06 0.85 176 51 202 152 257.98 1.63 177 51 20 160 257.45 1.57 178 51 210 161 257.36 1.59 179 52 61 9 443.10 1.47 180 52 66 14 443.08 1.46 181 52 209 158 257.51 1.56 182 52 20 159 257.46 1.57 183 52 210 160 257.43 1.57 184 52 211 161 257.41 1.58 185 56 59 3 802.87 6.84 186 60 63 3 829.22 3.46 187 61 64 3 826.38 2.54 188 61 209 149 257.58 1.61 189 65 211 148 257.21 1.68 190 65 223 175 118.67 0.70 191 66 76 10 574.28 24.50 192 66 77 11 518.86 2.94 193 66 78 12 518.76 2.98 194 66 85 19 518.21 2.81 195 66 211 147 257.21 1.67 196 67 76 9 574.13 24.57 197 67 77 10 521.22 1.84 198 67 85 18 534.64 2.10 199 67 211 146 257.27 1.65 200 67 213 148 257.07 1.66 201 67 221 171 126.27 1.57 202 67 223 173 114.68 2.71 203 68 75 7 589.31 18.54 204 68 76 8 582.27 20.51 205 68 85 17 539.10 0.12 206 68 89 21 544.59 1.82 207 68 93 25 555.67 1.72 208 68 221 170 126.26 1.57 209 69 72 3 864.81 1.30 210 69 74 5 627.63 0.78 211 69 75 6 591.87 17.20 212 69 93 24 555.68 1.72 213 69 221 169 126.20 1.60 214 70 74 4 702.20 15.64 215 70 75 5 627.54 0.81 216 70 76 6 594.00 17.57 217 70 89 19 555.62 1.72 218 70 92 22 555.66 1.72 219 70 93 23 555.66 1.72 220 70 112 43 589.37 15.13 221 70 138 69 555.56 1.65 222 71 74 3 817.27 7.00 223 71 96 25 608.89 5.37 224 71 106 36 614.79 3.35 225 71 107 37 614.55 3.48 226 71 112 42 601.39 9.78 227 72 106 35 615.22 3.16 228 72 219 164 126.50 1.49 229 72 220 165 126.51 1.50 230 72 221 166 126.26 1.61 231 73 140 68 608.25 0.36 232 73 141 69 608.24 0.35 233 73 215 144 257.18 1.68 234 73 219 163 126.51 1.49 235 73 220 164 126.48 1.50 236 73 221 165 126.20 1.60 237 74 107 34 615.48 3.21 238 74 108 35 615.48 3.21 239 74 109 36 615.38 3.26 240 74 112 39 615.36 3.23 241 74 138 65 608.62 0.32 242 74 139 66 608.58 0.33 243 74 140 67 608.27 0.38 244 74 215 143 257.01 1.71 245 75 138 64 608.63 0.31 246 75 139 65 608.60 0.33 247 75 215 142 256.97 1.67 248 75 221 163 126.20 1.59 249 76 85 9 616.32 3.04 250 76 137 62 608.88 0.33 251 76 138 63 608.63 0.31 252 77 85 8 616.75 3.22 253 77 136 60 609.09 0.24 254 77 137 61 608.89 0.33 255 78 81 3 1030.00 0.00 256 78 82 4 851.95 0.88 257 78 85 7 616.38 3.03 258 78 134 57 613.25 1.51 259 78 135 58 610.38 0.10 260 78 136 59 609.10 0.24 261 78 165 89 340.69 0.79 262 79 134 56 613.28 1.50 263 79 135 57 613.25 1.51 264 79 165 88 340.14 0.76 265 79 166 89 340.06 0.75 266 80 133 54 615.03 1.90 267 80 134 55 613.85 1.73 268 81 84 3 836.52 3.98 269 81 85 4 818.21 0.70 270 81 130 50 621.26 0.84 271 81 133 53 615.23 1.99 272 81 134 54 615.03 1.90 273 82 85 3 818.82 0.39 274 82 86 4 818.83 0.40 275 83 86 3 818.85 0.40 276 83 87 4 819.22 0.58 277 84 87 3 844.25 1.47 278 84 88 4 818.83 0.38 279 84 130 47 621.57 1.04 280 84 132 49 620.37 0.52 281 84 133 50 620.37 0.52 282 84 134 51 620.33 0.51 283 85 88 3 819.23 0.58 284 85 89 4 815.02 1.52 285 85 134 50 619.15 1.09 286 85 136 52 615.95 2.69 287 86 89 3 819.22 0.58 288 86 90 4 811.16 0.41 289 87 90 3 821.26 0.42 290 87 91 4 812.05 3.08 291 87 132 46 621.57 1.09 292 88 91 3 822.34 0.11 293 88 92 4 810.13 4.04 294 88 116 29 623.18 0.50 295 88 119 32 623.19 0.51 296 88 120 33 623.11 0.54 297 88 132 45 621.57 1.11 298 88 134 47 621.25 1.21 299 88 136 49 615.20 3.08 300 89 92 3 819.31 0.55 301 89 93 4 809.00 2.68 302 90 93 3 820.00 0.21 303 90 94 4 813.71 0.33 304 91 94 3 827.50 3.48 305 91 95 4 813.73 0.32 306 91 119 29 623.25 0.53 307 92 95 3 832.22 3.44 308 92 96 4 813.75 0.33 309 92 112 21 623.58 0.54 310 92 116 25 623.25 0.53 311 93 96 3 836.47 0.61 312 93 97 4 815.63 1.27 313 94 97 3 827.79 3.72 314 94 98 4 817.38 1.48 315 95 98 3 829.54 4.60 316 95 99 4 817.38 1.48 317 95 112 18 623.69 0.58 318 95 115 21 623.59 0.58 319 96 100 4 796.12 12.11 320 96 101 5 752.88 6.94 321 96 107 12 624.08 0.57 322 96 112 17 623.69 0.58 323 97 100 3 829.55 4.61 324 97 101 4 745.85 3.42 325 97 102 5 710.67 10.10 326 97 106 10 626.43 0.27 327 98 102 4 719.95 9.53 328 100 10 3 811.64 0.67 329 100 103 4 732.07 12.55 330 100 105 6 627.36 0.35 331 100 106 7 626.48 0.27 332 100 107 8 626.43 0.26 333 100 108 9 626.45 0.27 334 100 111 12 625.59 0.23 335 101 104 4 631.57 2.05 336 101 105 5 626.56 0.23 337 101 106 6 626.46 0.26 338 107 112 5 799.82 3.41 339 108 111 3 841.32 2.89 340 108 112 4 819.34 0.61 341 108 142 34 614.25 3.33 342 108 145 37 608.61 0.48 343 109 112 3 829.72 2.25 344 109 113 4 813.98 2.06 345 109 138 29 615.92 3.88 346 109 140 31 615.51 3.78 347 109 141 32 614.74 3.41 348 109 142 33 614.70 3.42 349 109 14 39 608.52 0.44 350 109 149 41 608.40 0.41 351 110 113 3 819.99 0.34 352 110 114 4 808.57 0.64 353 110 145 35 608.86 0.52 354 110 149 40 608.49 0.45 355 111 114 3 813.95 2.05 356 111 115 4 809.16 0.94 357 112 115 3 815.75 1.66 358 112 116 4 815.72 1.65 359 113 116 3 816.52 1.28 360 113 117 4 815.74 1.66 361 113 138 25 616.05 3.81 362 113 149 37 608.40 0.41 363 113 151 39 608.00 0.41 364 113 157 45 516.24 3.22 365 114 117 3 815.75 1.67 366 114 118 4 815.75 1.67 367 114 157 44 517.39 2.65 368 115 118 3 822.59 1.34 369 115 119 4 819.49 0.22 370 116 119 3 826.39 0.03 371 116 120 4 819.49 0.23 372 116 136 20 616.23 3.77 373 116 138 22 616.18 3.77 374 117 120 3 819.50 0.23 375 117 121 4 819.29 0.33 376 117 151 35 608.30 0.41 377 117 155 39 526.55 1.16 378 117 157 41 517.41 2.64 379 118 121 3 820.75 0.40 380 118 122 4 819.28 0.33 381 119 122 3 830.20 2.04 382 119 123 4 824.72 4.77 383 119 132 13 622.50 1.56 384 120 123 3 827.10 3.58 385 120 124 4 816.87 0.85 386 120 132 12 622.40 1.53 387 120 134 14 622.36 1.54 388 120 136 16 621.90 1.69 389 120 151 32 608.38 0.38 390 120 152 33 608.34 0.39 391 120 153 34 607.88 0.39 392 120 155 36 533.95 2.51 393 121 124 3 823.04 2.69 394 121 125 4 816.87 0.85 395 121 154 34 607.87 0.40 396 121 155 35 592.73 1.66 397 122 125 3 833.11 7.29 398 122 126 4 795.57 5.55 399 123 129 6 624.34 0.70 400 123 130 7 624.34 0.69 401 123 131 8 622.57 1.54 402 123 132 9 622.60 1.55 403 124 131 7 622.60 1.55 404 124 153 30 608.37 0.38 405 126 129 3 861.76 3.25 406 130 133 3 1030.00 0.00 407 132 153 22 607.98 0.37 408 134 153 20 607.98 0.37 409 135 151 17 608.04 0.39 410 135 152 18 607.98 0.41 411 135 153 19 607.94 0.39 412 135 165 31 340.14 0.76 413 136 149 14 608.31 0.37 414 136 150 15 608.04 0.39 415 136 151 16 608.01 0.39 416 137 148 12 608.31 0.36 417 137 149 13 608.08 0.38 418 137 150 14 608.02 0.39 419 137 163 27 341.22 0.93 420 137 165 29 340.72 0.79 421 138 148 11 608.21 0.35 422 138 149 12 608.03 0.39 423 138 151 14 608.01 0.39 424 139 14 9 608.23 0.34 425 139 148 10 608.04 0.41 426 139 215 77 256.94 1.68 427 140 147 7 608.35 0.35 428 140 14 8 608.26 0.32 429 140 215 76 257.00 1.71 430 141 146 5 608.54 0.43 431 141 147 6 608.27 0.32 432 141 215 75 257.08 1.74 433 141 220 95 126.49 1.50 434 142 145 3 813.58 1.74 435 142 146 4 655.36 13.12 436 147 215 69 257.08 1.74 437 147 216 70 257.02 1.72 438 148 159 11 465.14 1.17 439 148 160 12 422.75 19.87 440 148 162 14 380.86 0.73 441 149 157 8 477.87 7.13 442 149 158 9 467.14 2.00 443 149 159 10 422.92 19.82 444 150 157 7 477.81 7.11 445 150 158 8 467.28 1.94 446 150 160 10 423.80 19.39 447 150 165 15 340.16 0.76 448 151 156 5 577.50 33.95 449 151 157 6 477.81 7.11 450 152 155 3 823.62 1.60 451 152 156 4 595.31 33.23 452 152 158 6 586.97 37.40 453 160 163 3 861.75 0.83 454 161 180 19 308.24 1.23 455 162 176 14 330.93 1.93 456 162 180 18 308.31 1.26 457 163 173 10 332.82 1.58 458 163 176 13 330.88 1.97 459 164 169 5 339.81 0.68 460 164 172 8 333.00 1.64 461 164 173 9 332.33 1.32 462 164 176 12 330.87 1.97 463 165 169 4 496.88 78.23 464 166 169 3 810.36 4.38 465 168 171 3 825.85 2.00 466 168 172 4 802.84 1.45 467 169 172 3 808.95 1.59 468 169 173 4 807.95 1.10 469 170 173 3 823.19 4.91 470 170 174 4 814.53 2.19 471 171 174 3 821.70 1.38 472 171 175 4 814.58 2.19 473 172 175 3 821.71 1.38 474 172 176 4 814.58 2.19 475 172 196 24 259.79 0.91 476 173 176 3 821.71 1.38 477 173 177 4 815.16 2.48 478 174 177 3 824.89 0.21 479 174 178 4 815.17 2.48 480 175 178 3 829.88 2.44 481 175 179 4 815.16 2.48 482 175 195 20 260.17 0.88 483 175 196 21 259.80 0.90 484 176 179 3 822.58 1.22 485 176 180 4 804.76 7.13 486 176 192 16 260.23 0.89 487 176 196 20 259.75 0.88 488 179 192 13 260.38 0.82 489 179 195 16 260.33 0.80 490 180 184 4 809.12 0.33 491 181 184 3 846.32 3.14 492 184 188 4 797.79 1.39 493 185 188 3 807.10 0.98 494 185 189 4 805.29 1.89 495 186 189 3 806.61 1.26 496 186 190 4 802.65 3.21 497 186 214 28 257.42 1.59 498 186 216 30 256.94 1.67 499 187 190 3 816.93 3.92 500 187 191 4 816.93 3.93 501 187 232 60 90.44 0.36 502 188 191 3 816.94 3.92 503 188 192 4 816.93 3.93 504 189 192 3 820.11 2.33 505 189 193 4 812.62 6.08 506 190 193 3 820.10 2.33 507 190 194 4 812.24 1.65 508 190 212 22 257.47 1.58 509 190 214 24 257.28 1.57 510 190 232 57 88.90 0.30 511 191 194 3 815.58 0.02 512 191 195 4 815.58 0.02 513 191 235 59 82.78 0.24 514 191 236 60 47.13 8.89 515 192 195 3 817.10 0.78 516 192 196 4 815.57 0.02 517 193 196 3 817.28 0.69 518 193 197 4 820.79 2.64 519 193 212 19 257.37 1.58 520 194 197 3 820.96 2.56 521 194 198 4 820.99 2.54 522 194 206 11 257.73 1.59 523 194 210 16 257.48 1.58 524 194 212 18 257.31 1.59 525 194 229 50 93.41 1.36 526 194 232 53 87.97 0.06 527 194 233 54 87.48 0.18 528 194 236 57 43.43 7.25 529 195 198 3 826.58 0.24 530 195 199 4 823.16 1.47 531 195 236 56 43.43 7.25 532 196 199 3 834.63 1.03 533 197 200 3 826.58 0.24 534 197 202 4 817.86 4.09 535 197 206 8 257.60 1.57 536 197 210 13 257.39 1.58 537 198 202 3 818.40 4.35 538 198 206 7 257.56 1.57 539 198 236 53 43.43 7.25 540 202 205 3 915.80 1.07 541 202 206 4 804.48 5.82 542 205 20 5 686.40 13.08 543 206 20 4 747.04 6.50 544 206 210 5 686.40 13.08 545 206 233 43 87.60 0.12 546 207 20 3 830.62 3.06 547 207 210 4 747.04 6.50 548 207 230 39 93.04 1.55 549 207 233 42 87.61 0.12 550 208 226 34 93.50 1.34 551 208 229 37 93.46 1.35 552 208 230 38 93.05 1.55 553 208 233 41 88.13 0.14 554 209 226 33 93.50 1.33 555 210 229 34 93.45 1.35 556 210 233 38 87.61 0.12 557 211 223 27 116.98 1.56 558 212 223 26 115.59 2.25 559 212 224 27 114.62 2.72 560 212 229 32 93.54 1.30 561 213 221 23 126.42 1.50 562 213 222 24 126.03 1.56 563 213 223 25 117.19 1.46 564 213 224 26 114.68 2.71 565 214 220 21 126.50 1.49 566 214 221 22 126.41 1.50 567 214 222 23 126.10 1.55 568 214 224 25 125.64 1.44 569 215 220 20 126.47 1.50 570 215 221 21 126.41 1.49 571 216 220 19 126.51 1.51 572 217 220 18 126.63 1.46 573 21 21 14 128.35 1.69 574 21 218 15 128.25 1.74 575 21 219 16 128.16 1.74 576 21 220 17 127.55 1.89 577 21 21 3 812.98 5.61 578 21 21 4 798.25 0.67 579 21 21 13 128.60 1.65 580 21 218 14 128.28 1.72 581 21 219 15 128.25 1.73 582 21 220 16 128.22 1.74 583 21 222 18 127.98 1.70 584 21 21 3 816.43 4.86 585 21 21 12 128.56 1.67 586 21 218 13 128.34 1.73 587 21 219 12 128.56 1.62 588 21 222 15 128.28 1.72 589 21 21 8 128.71 1.64 590 21 218 10 128.60 1.65 591 21 219 11 128.60 1.65 592 21 21 3 1030.00 0.00 593 21 21 6 701.10 16.64 594 21 21 3 821.76 3.70 595 21 21 3 877.10 0.10 596 222 228 6 804.94 2.10 597 224 228 4 804.94 2.10 598 224 229 5 786.72 5.78 599 224 232 8 781.24 3.02 600 225 228 3 813.39 1.28 601 225 229 4 808.27 0.45 602 226 229 3 813.81 1.50 603 226 230 4 808.30 0.46 604 227 230 3 823.89 3.65 605 227 231 4 808.47 0.55 606 228 231 3 818.13 0.78 607 228 232 4 808.66 0.45 608 229 232 3 822.21 2.81 609 229 233 4 808.66 0.45 610 230 233 3 810.77 0.61 611 230 234 4 802.60 2.59 612 231 234 3 831.69 2.17 613 232 235 3 816.70 2.37 614 232 236 4 797.40 5.17 615 233 236 3 806.08 2.94