BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 45 44 22.57 0.22 2 1 46 45 22.59 0.21 3 1 47 46 22.55 0.23 4 1 49 48 27.43 0.36 5 1 97 96 24.69 0.95 6 1 98 97 24.73 0.97 7 2 24 22 24.32 0.93 8 2 48 46 24.45 0.88 9 2 49 47 28.59 0.58 10 3 49 46 28.59 0.58 11 3 51 48 37.20 0.17 12 4 17 13 38.91 0.34 13 4 20 16 31.79 0.52 14 4 21 17 31.78 0.52 15 4 24 20 31.78 0.52 16 4 48 44 31.94 0.44 17 4 49 45 32.18 0.32 18 4 50 46 33.63 0.41 19 4 51 47 38.02 0.05 20 5 17 12 39.45 0.41 21 5 51 46 38.48 0.27 22 6 10 4 839.90 6.09 23 6 11 5 822.13 3.75 24 6 13 7 49.20 0.26 25 6 14 8 40.68 0.03 26 6 17 11 39.52 0.43 27 6 51 45 40.81 0.11 28 6 52 46 42.11 0.54 29 6 53 47 51.28 0.89 30 6 132 126 62.03 1.18 31 7 10 3 841.36 5.37 32 7 53 46 55.51 0.77 33 8 52 44 61.50 1.45 34 8 53 45 62.11 1.27 35 8 54 46 63.51 1.35 36 8 55 47 70.74 0.60 37 8 132 124 62.05 1.18 38 8 135 127 62.95 1.62 39 8 136 128 65.27 0.48 40 8 139 131 66.40 0.02 41 9 55 46 75.16 1.60 42 10 13 3 861.82 0.49 43 11 14 3 861.89 0.53 44 11 132 121 82.53 0.48 45 11 181 170 80.03 0.74 46 12 15 3 921.14 2.35 47 12 182 170 92.23 0.48 48 13 16 3 861.88 0.53 49 13 17 4 831.32 1.69 50 14 17 3 838.97 1.93 51 14 18 4 831.32 1.69 52 14 162 148 90.91 0.87 53 14 166 152 84.81 0.24 54 14 173 159 85.59 0.10 55 14 176 162 91.40 0.89 56 14 177 163 91.86 0.66 57 14 181 167 92.56 0.38 58 15 18 3 838.56 1.92 59 15 19 4 831.62 1.54 60 15 173 158 90.11 1.28 61 15 177 162 92.36 0.46 62 16 19 3 846.68 2.25 63 16 20 4 831.63 1.54 64 17 20 3 846.79 2.20 65 17 21 4 839.87 5.66 66 17 50 33 99.45 0.86 67 17 163 146 96.59 1.43 68 18 21 3 839.88 5.67 69 18 22 4 836.66 3.04 70 18 162 144 97.42 1.79 71 18 163 145 96.72 1.49 72 18 166 148 93.23 0.06 73 18 167 149 92.27 0.50 74 18 173 155 93.69 0.26 75 19 22 3 852.55 0.67 76 19 23 4 837.00 2.88 77 20 23 3 850.70 1.90 78 20 24 4 837.00 2.88 79 21 24 3 848.08 2.66 80 21 25 4 853.57 0.29 81 21 48 27 109.28 1.06 82 21 50 29 110.00 0.76 83 21 64 43 109.33 0.59 84 21 163 142 109.48 0.58 85 21 167 146 107.98 0.62 86 22 25 3 853.77 0.20 87 22 26 4 847.85 2.56 88 22 166 144 109.56 0.60 89 22 167 145 109.14 0.47 90 22 169 147 109.41 0.56 91 22 170 148 109.49 0.58 92 22 173 151 109.81 0.53 93 22 242 220 102.81 1.96 94 23 26 3 861.85 0.86 95 24 27 3 924.72 8.58 96 24 48 24 110.37 0.89 97 25 37 12 109.42 0.56 98 25 48 23 110.50 0.85 99 25 64 39 110.45 0.84 100 25 66 41 109.98 0.74 101 25 69 44 110.03 0.72 102 25 167 142 110.38 0.80 103 25 243 218 110.11 0.69 104 26 35 9 109.97 0.75 105 26 36 10 109.47 0.57 106 26 243 217 110.29 0.77 107 27 36 9 110.42 0.83 108 27 38 11 110.37 0.90 109 27 41 14 110.39 0.91 110 27 66 39 110.50 0.85 111 28 36 8 110.45 0.84 112 28 38 10 110.39 0.91 113 29 36 7 110.63 0.79 114 30 34 4 598.13 67.90 115 30 36 6 110.69 0.76 116 31 34 3 840.69 0.55 117 31 36 5 458.37 119.12 118 31 205 174 110.81 0.69 119 31 206 175 110.73 0.71 120 31 244 213 110.92 0.69 121 32 205 173 127.42 7.61 122 32 208 176 127.52 7.67 123 32 209 177 127.50 7.66 124 32 212 180 127.48 7.66 125 32 234 202 140.88 2.00 126 34 205 171 141.80 1.61 127 34 238 204 141.87 1.64 128 34 241 207 141.88 1.61 129 34 244 210 141.77 1.58 130 35 242 207 141.95 1.58 131 35 243 208 141.81 1.59 132 35 244 209 141.81 1.58 133 36 206 170 141.90 1.62 134 36 243 207 141.88 1.58 135 36 244 208 141.81 1.58 136 36 245 209 141.80 1.58 137 36 246 210 141.19 1.33 138 37 66 29 141.98 1.56 139 37 67 30 141.97 1.57 140 37 69 32 141.99 1.56 141 37 198 161 142.00 1.57 142 37 243 206 142.02 1.58 143 37 244 207 141.88 1.58 144 37 245 208 141.82 1.58 145 37 246 209 141.71 1.57 146 38 41 3 893.02 4.03 147 38 67 29 141.99 1.56 148 38 246 208 141.81 1.58 149 39 67 28 388.12 121.47 150 39 246 207 141.83 1.59 151 40 67 27 388.24 121.50 152 41 45 4 839.87 11.34 153 41 46 5 679.21 8.54 154 41 66 25 397.44 125.87 155 41 67 26 397.35 125.89 156 42 45 3 849.61 6.49 157 42 46 4 775.28 20.96 158 43 46 3 820.63 6.72 159 43 93 50 410.94 110.17 160 43 97 54 411.50 109.88 161 45 66 21 439.37 123.86 162 46 65 19 439.41 123.87 163 46 66 20 439.37 123.86 164 46 67 21 439.37 123.86 165 46 91 45 434.69 121.42 166 46 93 47 434.50 121.20 167 47 64 17 439.43 123.87 168 47 65 18 439.42 123.87 169 47 66 19 439.42 123.86 170 47 72 25 439.15 123.70 171 47 93 46 434.82 121.34 172 47 94 47 434.79 121.33 173 47 98 51 434.00 120.92 174 48 63 15 439.79 123.82 175 48 64 16 439.54 123.84 176 48 66 18 439.50 123.83 177 49 62 13 439.91 123.86 178 49 63 14 439.79 123.82 179 49 98 49 435.25 121.14 180 49 99 50 435.37 121.03 181 50 60 10 440.84 123.62 182 50 61 11 440.58 123.63 183 50 62 12 440.06 123.79 184 50 64 14 440.06 123.79 185 50 163 113 319.87 63.83 186 51 59 8 440.92 123.60 187 51 60 9 440.82 123.62 188 51 61 10 440.60 123.63 189 52 59 7 440.93 123.60 190 52 60 8 440.85 123.62 191 52 62 10 440.75 123.62 192 52 135 83 351.76 78.98 193 52 139 87 353.00 79.59 194 52 159 107 344.48 75.55 195 52 163 111 326.11 66.61 196 53 57 4 795.33 10.98 197 53 58 5 442.42 124.31 198 53 59 6 440.92 123.61 199 54 57 3 818.70 0.70 200 54 58 4 596.34 65.14 201 54 136 82 351.79 78.98 202 54 139 85 353.67 79.28 203 55 136 81 353.53 79.20 204 56 136 80 422.25 44.99 205 56 140 84 446.26 33.66 206 58 140 82 457.79 27.88 207 58 143 85 463.56 25.49 208 59 143 84 467.73 23.43 209 60 75 15 556.25 64.91 210 61 74 13 556.43 64.92 211 61 75 14 556.29 64.89 212 61 99 38 553.66 61.99 213 62 71 9 558.28 64.36 214 62 73 11 557.97 64.22 215 62 74 12 556.50 64.78 216 62 155 93 468.17 20.81 217 62 159 97 458.99 16.27 218 63 71 8 558.25 64.34 219 63 72 9 558.11 64.28 220 63 73 10 558.08 64.27 221 63 98 35 554.40 61.55 222 63 99 36 554.41 61.55 223 64 69 5 558.34 64.36 224 64 70 6 558.29 64.33 225 64 71 7 558.10 64.27 226 64 163 99 438.56 4.46 227 64 164 100 438.54 4.46 228 65 68 3 860.59 1.32 229 65 69 4 609.25 38.81 230 65 70 5 558.20 64.27 231 65 72 7 558.43 64.09 232 65 91 26 554.57 61.56 233 67 246 179 190.37 23.62 234 68 168 100 434.22 1.11 235 68 198 130 308.71 34.99 236 69 164 95 439.71 3.88 237 69 167 98 434.22 1.12 238 69 168 99 434.20 1.11 239 69 198 129 308.77 35.01 240 70 168 98 435.88 1.86 241 71 87 16 563.70 57.46 242 71 156 85 470.78 19.34 243 71 160 89 453.27 10.57 244 71 164 93 439.81 3.83 245 72 86 14 563.82 57.40 246 72 87 15 563.70 57.45 247 72 90 18 563.71 57.30 248 72 91 19 563.81 57.26 249 72 94 22 564.12 57.05 250 72 98 26 568.00 54.81 251 73 85 12 563.98 57.34 252 73 86 13 563.82 57.40 253 73 156 83 479.29 15.30 254 74 84 10 567.62 55.55 255 74 85 11 564.46 57.14 256 74 99 25 568.81 54.48 257 74 142 68 486.79 14.42 258 74 152 78 481.81 16.35 259 74 155 81 480.51 15.84 260 74 156 82 479.50 15.35 261 75 82 7 569.58 54.84 262 75 83 8 569.15 54.95 263 75 84 9 567.62 55.56 264 75 99 24 568.83 54.48 265 75 102 27 568.45 54.23 266 76 82 6 569.61 54.84 267 76 83 7 569.55 54.86 268 76 143 67 486.85 14.38 269 76 144 68 487.85 14.47 270 77 81 4 749.78 28.76 271 77 82 5 569.61 54.84 272 77 83 6 569.61 54.84 273 77 143 66 487.88 14.46 274 77 144 67 488.09 14.51 275 78 81 3 842.38 2.15 276 78 83 5 575.84 57.95 277 78 104 26 656.61 10.13 278 78 144 66 488.36 14.37 279 78 145 67 488.57 14.36 280 78 146 68 488.65 14.36 281 82 102 20 739.08 30.90 282 82 104 22 738.87 30.99 283 83 102 19 739.11 30.91 284 83 104 21 737.98 31.43 285 83 106 23 737.97 31.44 286 83 142 59 552.14 17.51 287 83 144 61 552.14 17.51 288 83 145 62 558.18 20.45 289 83 147 64 558.95 20.80 290 84 99 15 739.46 30.75 291 84 102 18 738.92 31.02 292 84 104 20 738.01 31.45 293 84 105 21 713.46 43.70 294 84 106 22 708.24 46.30 295 85 106 21 698.89 50.98 296 85 108 23 689.27 55.78 297 85 147 62 573.25 28.34 298 86 90 4 837.01 1.91 299 86 94 8 739.80 30.70 300 86 95 9 739.76 30.71 301 86 98 12 739.58 30.80 302 86 99 13 739.55 30.80 303 86 105 19 713.51 43.72 304 86 106 20 698.74 51.06 305 86 107 21 689.00 55.93 306 86 108 22 688.83 56.01 307 87 90 3 877.75 1.01 308 87 108 21 676.35 62.26 309 87 110 23 667.49 66.58 310 87 111 24 643.85 64.36 311 87 112 25 632.40 58.66 312 87 117 30 579.91 32.37 313 87 156 69 552.26 18.84 314 88 108 20 676.35 62.26 315 88 109 21 676.33 62.26 316 88 110 22 667.49 66.59 317 88 111 23 657.00 70.94 318 89 111 22 657.80 71.34 319 90 94 4 833.06 0.85 320 90 95 5 826.93 3.90 321 91 94 3 838.89 0.22 322 91 95 4 837.69 0.34 323 92 95 3 852.96 0.84 324 92 96 4 827.61 4.22 325 93 96 3 845.84 4.41 326 93 97 4 827.61 4.22 327 94 97 3 855.87 5.42 328 94 98 4 822.74 5.78 329 95 105 10 700.37 50.31 330 97 100 3 855.06 2.28 331 98 105 7 751.45 25.20 332 99 102 3 876.03 9.08 333 99 105 6 783.33 9.26 334 102 105 3 911.50 0.21 335 106 147 41 574.53 27.95 336 108 147 39 575.66 27.86 337 108 152 44 574.57 27.96 338 108 156 48 574.56 27.96 339 110 116 6 701.35 28.36 340 110 117 7 681.25 4.94 341 110 120 10 581.96 30.56 342 110 149 39 576.10 27.89 343 110 152 42 576.28 27.80 344 110 153 43 576.27 27.80 345 111 116 5 779.80 10.85 346 111 117 6 795.65 2.93 347 112 117 5 806.50 2.23 348 113 116 3 849.38 1.06 349 113 117 4 838.77 6.37 350 114 117 3 843.26 4.12 351 114 118 4 835.48 8.01 352 114 125 11 581.60 25.31 353 114 157 43 576.43 27.74 354 114 160 46 575.96 27.51 355 114 161 47 575.95 27.50 356 114 165 51 532.98 28.44 357 114 195 81 376.22 56.53 358 115 118 3 861.45 3.03 359 115 119 4 825.27 6.73 360 116 119 3 850.88 0.30 361 116 120 4 834.65 7.20 362 117 120 3 848.77 3.31 363 117 121 4 812.64 0.42 364 117 153 36 578.28 27.64 365 117 156 39 577.18 28.00 366 117 157 40 577.17 28.00 367 117 160 43 577.18 28.00 368 118 121 3 838.82 0.92 369 118 122 4 801.54 5.38 370 118 123 5 722.82 12.61 371 118 124 6 605.00 32.81 372 118 125 7 603.49 33.57 373 118 157 39 578.58 27.50 374 119 122 3 835.71 10.54 375 120 150 30 579.08 27.56 376 120 153 33 579.04 27.55 377 121 150 29 580.07 27.07 378 121 153 32 579.07 27.56 379 121 154 33 579.04 27.55 380 121 157 36 579.04 27.55 381 122 150 28 580.38 27.14 382 123 127 4 811.88 0.29 383 123 128 5 729.57 21.03 384 123 154 31 580.45 27.13 385 123 157 34 580.39 27.15 386 124 127 3 846.10 1.04 387 124 128 4 816.38 4.18 388 125 128 3 838.75 4.18 389 125 157 32 580.45 27.14 390 125 161 36 580.42 27.14 391 125 187 62 396.60 63.63 392 125 192 67 388.35 59.76 393 125 195 70 387.28 59.21 394 125 196 71 387.28 59.21 395 126 186 60 397.69 63.16 396 126 187 61 396.67 63.66 397 126 192 66 388.82 59.94 398 127 186 59 397.69 63.17 399 128 154 26 582.45 26.45 400 128 157 29 582.40 26.45 401 128 158 30 582.38 26.46 402 128 186 58 396.79 63.62 403 128 187 59 396.72 63.64 404 129 134 5 804.82 0.46 405 129 158 29 582.43 26.45 406 129 184 55 398.23 63.15 407 129 185 56 398.12 63.18 408 129 186 57 396.79 63.62 409 130 134 4 804.84 0.47 410 130 158 28 582.43 26.45 411 130 161 31 582.41 26.45 412 130 162 32 582.39 26.46 413 130 184 54 398.18 63.16 414 130 185 55 397.79 63.13 415 130 187 57 396.68 63.67 416 131 134 3 847.59 4.64 417 131 135 4 830.37 2.51 418 131 162 31 582.43 26.45 419 131 180 49 398.75 62.89 420 131 183 52 398.67 62.95 421 132 135 3 842.96 0.94 422 132 136 4 838.51 1.51 423 132 162 30 582.47 26.45 424 132 180 48 398.79 62.91 425 132 181 49 398.79 62.91 426 133 136 3 847.37 4.53 427 133 137 4 838.52 1.51 428 134 137 3 838.53 1.50 429 134 138 4 838.53 1.50 430 134 158 24 582.79 26.45 431 134 184 50 398.19 63.16 432 135 138 3 842.71 1.20 433 135 139 4 838.52 1.50 434 135 155 20 582.88 26.48 435 135 158 23 582.78 26.44 436 135 159 24 582.77 26.44 437 135 162 27 582.66 26.39 438 136 139 3 840.91 0.30 439 136 140 4 838.51 1.50 440 137 140 3 848.47 4.08 441 137 141 4 848.47 4.08 442 138 141 3 848.47 4.08 443 138 142 4 846.14 5.24 444 138 155 17 582.90 26.49 445 138 158 20 582.90 26.49 446 139 142 3 848.46 4.08 447 139 143 4 838.94 8.84 448 139 155 16 583.00 26.46 449 140 145 5 839.23 8.69 450 141 145 4 843.02 2.39 451 141 151 10 583.21 26.43 452 142 145 3 836.41 0.92 453 142 151 9 583.21 26.43 454 142 152 10 583.19 26.44 455 142 155 13 583.19 26.43 456 147 152 5 828.71 2.59 457 148 151 3 868.07 6.47 458 148 152 4 834.97 0.54 459 149 152 3 857.32 0.90 460 149 153 4 831.47 2.52 461 150 153 3 849.27 6.21 462 150 154 4 838.45 0.96 463 151 154 3 852.98 4.35 464 151 155 4 841.97 0.80 465 152 155 3 846.99 1.71 466 152 156 4 836.51 3.52 467 153 156 3 847.29 1.85 468 153 157 4 837.27 3.15 469 154 157 3 849.38 2.99 470 154 158 4 843.17 6.09 471 155 158 3 853.22 1.07 472 155 159 4 832.12 0.58 473 156 159 3 857.16 0.17 474 156 160 4 832.09 0.59 475 157 161 4 837.25 2.25 476 158 161 3 861.40 2.23 477 158 162 4 838.85 3.05 478 159 162 3 847.00 4.98 479 159 163 4 838.85 3.06 480 160 163 3 859.00 1.02 481 160 164 4 761.34 25.48 482 160 165 5 751.97 21.03 483 161 165 4 744.74 24.63 484 161 166 5 755.34 19.63 485 161 172 11 474.36 25.52 486 161 187 26 395.48 64.25 487 161 195 34 376.06 56.63 488 162 166 4 774.71 29.32 489 162 176 14 454.67 34.97 490 163 166 3 851.76 2.59 491 163 167 4 819.12 7.13 492 164 167 3 853.66 1.64 493 164 168 4 823.31 5.04 494 165 168 3 847.40 4.76 495 165 169 4 819.12 7.13 496 165 172 7 723.59 34.19 497 165 195 30 376.14 56.70 498 166 169 3 846.33 6.46 499 166 172 6 675.63 15.85 500 166 173 7 470.81 27.25 501 167 242 75 220.18 15.78 502 167 243 76 219.87 15.93 503 168 198 30 374.05 57.63 504 168 241 73 222.72 16.77 505 168 242 74 220.18 15.78 506 168 243 75 219.51 15.94 507 169 172 3 844.37 5.47 508 169 173 4 844.39 5.46 509 169 194 25 385.66 61.41 510 169 195 26 385.81 61.34 511 169 198 29 374.24 57.66 512 169 201 32 318.92 30.00 513 169 240 71 222.84 16.75 514 169 241 72 222.72 16.77 515 169 242 73 221.61 16.30 516 170 173 3 846.83 4.24 517 170 174 4 844.38 5.46 518 170 239 69 223.00 16.67 519 170 240 70 222.84 16.75 520 170 241 71 222.84 16.75 521 170 242 72 222.22 16.52 522 171 174 3 852.24 1.54 523 171 175 4 844.39 5.45 524 171 188 17 395.60 64.23 525 171 190 19 393.88 64.70 526 171 191 20 393.42 64.46 527 171 194 23 392.90 64.23 528 171 224 53 296.20 15.34 529 171 240 69 222.99 16.68 530 172 175 3 844.40 5.45 531 172 176 4 844.38 5.45 532 172 191 19 392.92 64.23 533 172 194 22 386.64 61.10 534 172 195 23 386.55 61.05 535 173 176 3 847.75 0.70 536 173 177 4 825.84 0.24 537 174 177 3 847.12 0.39 538 174 178 4 827.11 0.40 539 175 178 3 852.45 2.27 540 175 179 4 825.25 0.48 541 175 180 5 824.18 1.00 542 175 185 10 398.35 63.23 543 175 188 13 396.86 63.76 544 175 191 16 394.60 64.37 545 176 179 3 833.20 3.35 546 176 180 4 809.69 5.88 547 176 181 5 809.69 5.88 548 176 185 9 399.56 63.86 549 177 181 4 812.04 7.07 550 179 183 4 730.23 29.18 551 179 184 5 630.21 50.09 552 179 185 6 536.51 3.25 553 180 183 3 841.91 0.07 554 180 185 5 535.17 3.92 555 187 191 4 808.47 8.37 556 187 192 5 808.45 8.38 557 188 191 3 848.24 4.51 558 188 192 4 832.67 3.28 559 188 224 36 346.59 9.86 560 189 192 3 841.47 1.11 561 189 193 4 833.12 3.50 562 189 222 33 346.57 9.87 563 189 223 34 346.59 9.86 564 189 224 35 346.60 9.86 565 189 225 36 346.61 9.86 566 189 226 37 346.59 9.86 567 190 193 3 841.22 0.55 568 190 194 4 833.12 3.50 569 190 222 32 346.57 9.88 570 190 223 33 346.58 9.87 571 190 224 34 346.61 9.87 572 190 229 39 346.56 9.88 573 190 240 50 223.56 16.57 574 191 194 3 846.83 0.30 575 191 195 4 833.47 3.33 576 192 195 3 840.91 0.39 577 192 196 4 838.38 0.87 578 193 196 3 843.44 1.68 579 193 197 4 837.14 0.03 580 193 221 28 346.60 9.89 581 193 222 29 346.62 9.90 582 194 197 3 852.63 2.93 583 194 198 4 830.66 3.27 584 194 201 7 735.57 10.16 585 194 237 43 223.94 16.70 586 197 200 3 842.68 1.30 587 197 201 4 842.06 0.34 588 197 221 24 346.66 9.92 589 198 201 3 842.07 0.34 590 198 202 4 842.07 0.33 591 198 241 43 222.98 16.83 592 198 243 45 222.68 16.82 593 198 244 46 219.66 16.02 594 198 245 47 218.88 16.09 595 199 202 3 842.07 0.33 596 199 203 4 842.07 0.33 597 199 245 46 219.03 16.21 598 200 203 3 842.07 0.33 599 200 204 4 834.24 4.24 600 200 219 19 346.84 9.99 601 201 204 3 851.49 3.58 602 201 205 4 842.01 0.34 603 201 219 18 346.81 10.02 604 201 221 20 346.91 9.97 605 201 237 36 271.18 6.65 606 201 241 40 223.54 16.59 607 202 205 3 858.60 0.03 608 202 206 4 838.95 1.20 609 202 244 42 220.63 16.12 610 202 245 43 219.77 15.97 611 203 206 3 856.43 1.09 612 203 207 4 838.94 1.20 613 203 245 42 222.00 16.81 614 203 247 44 219.05 16.19 615 204 207 3 839.35 1.08 616 204 208 4 839.03 1.25 617 204 215 11 346.84 9.98 618 204 219 15 346.91 9.97 619 205 208 3 847.90 2.66 620 205 209 4 844.88 4.17 621 205 237 32 311.63 21.40 622 205 241 36 280.55 11.46 623 205 244 39 223.05 16.79 624 206 209 3 858.18 5.51 625 206 245 39 222.93 16.85 626 206 246 40 222.91 16.84 627 206 247 41 219.83 16.34 628 206 248 42 219.06 16.20 629 207 216 9 347.09 10.04 630 207 217 10 347.12 10.03 631 208 211 3 908.83 0.57 632 208 212 4 687.70 14.97 633 208 214 6 350.68 8.95 634 208 215 7 347.02 10.05 635 208 234 26 346.92 10.10 636 209 212 3 843.34 4.95 637 212 234 22 347.06 10.08 638 213 233 20 347.13 10.04 639 213 234 21 347.12 10.04 640 214 232 18 347.29 10.09 641 215 218 3 873.70 1.40 642 215 219 4 858.21 1.19 643 215 231 16 347.38 10.12 644 215 232 17 347.29 10.10 645 215 233 18 347.29 10.10 646 215 234 19 347.29 10.09 647 215 237 22 346.75 10.36 648 218 230 12 348.08 9.97 649 218 231 13 347.56 10.19 650 218 232 14 347.54 10.20 651 219 230 11 348.17 9.98 652 219 231 12 347.89 10.03 653 220 230 10 348.18 9.97 654 221 228 7 348.46 9.97 655 221 229 8 348.28 9.97 656 221 231 10 348.27 9.97 657 221 237 16 348.23 9.98 658 222 226 4 650.89 57.49 659 222 227 5 348.56 9.96 660 222 229 7 348.30 9.96 661 223 226 3 826.44 6.48 662 223 227 4 464.48 67.23 663 223 229 6 348.51 9.95 664 229 236 7 543.39 88.84 665 229 237 8 531.45 94.16 666 229 240 11 356.36 7.80 667 231 236 5 709.37 21.64 668 231 237 6 640.70 55.98 669 232 236 4 843.90 3.06 670 233 236 3 852.48 1.23 671 233 237 4 847.17 3.77 672 234 237 3 855.14 0.10 673 234 238 4 838.70 0.46 674 235 239 4 841.09 0.73 675 236 239 3 901.23 2.90 676 237 240 3 861.04 1.56 677 237 241 4 836.99 4.80 678 238 241 3 851.91 0.85 679 241 244 3 911.59 2.10