BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 8 12 4 850.64 0.15 2 8 127 120 46.90 0.31 3 8 128 121 30.89 1.46 4 9 127 119 46.90 0.31 5 10 127 118 47.88 0.56 6 10 128 119 47.93 0.55 7 11 14 3 852.36 0.33 8 11 125 115 48.42 0.32 9 11 127 117 48.56 0.24 10 12 23 11 48.59 0.23 11 12 128 117 48.58 0.24 12 12 129 118 48.58 0.24 13 13 21 8 48.63 0.21 14 13 23 10 48.58 0.24 15 13 24 11 48.59 0.23 16 13 124 112 48.59 0.24 17 13 131 119 48.58 0.24 18 14 21 7 48.65 0.21 19 15 20 5 243.30 95.70 20 15 21 6 48.76 0.23 21 16 19 3 790.45 6.05 22 16 20 4 652.02 12.88 23 20 25 5 788.48 2.81 24 21 24 3 834.72 0.14 25 21 25 4 807.85 2.63 26 22 25 3 819.95 7.25 27 22 26 4 807.91 2.61 28 23 26 3 827.33 3.55 29 23 27 4 792.80 4.96 30 23 131 109 374.35 1.52 31 24 27 3 827.32 3.54 32 24 28 4 811.63 4.30 33 24 124 101 374.38 1.50 34 24 131 108 375.90 0.76 35 24 154 132 374.05 1.51 36 25 28 3 827.94 2.92 37 25 29 4 811.63 4.30 38 26 29 3 819.97 8.23 39 26 30 4 814.02 1.28 40 27 30 3 828.33 4.05 41 27 31 4 812.70 0.24 42 27 131 105 382.31 0.45 43 27 132 106 382.31 0.44 44 27 133 107 382.31 0.45 45 27 153 128 382.22 0.44 46 28 31 3 816.30 1.55 47 28 32 4 812.70 0.24 48 28 152 126 382.25 0.43 49 28 153 127 382.25 0.43 50 29 32 3 814.88 0.84 51 29 33 4 812.70 0.24 52 30 33 3 825.63 3.12 53 30 34 4 812.70 0.24 54 30 80 50 383.20 0.48 55 30 81 51 383.29 0.48 56 31 34 3 820.21 1.82 57 31 79 48 383.13 0.45 58 31 80 49 383.13 0.44 59 31 133 103 383.18 0.46 60 31 134 104 383.09 0.45 61 31 135 105 382.78 0.48 62 31 153 124 383.06 0.45 63 31 166 137 339.80 0.70 64 32 166 136 339.81 0.70 65 32 167 137 339.66 0.77 66 32 169 139 339.44 0.85 67 33 167 136 339.66 0.77 68 34 80 46 383.59 0.51 69 34 166 134 339.90 0.66 70 34 167 135 339.81 0.70 71 35 50 15 428.60 7.73 72 35 51 16 428.56 7.72 73 35 53 18 428.51 7.74 74 35 66 31 428.44 7.70 75 35 79 44 396.55 4.41 76 35 166 133 339.94 0.67 77 36 50 14 428.56 7.71 78 36 51 15 428.59 7.73 79 36 165 131 340.27 0.57 80 36 166 132 339.95 0.67 81 36 167 133 339.95 0.67 82 36 168 134 339.93 0.66 83 36 200 166 252.72 0.83 84 37 49 12 428.66 7.75 85 37 50 13 428.56 7.71 86 37 77 40 422.52 4.72 87 37 78 41 420.91 5.52 88 37 79 42 419.40 4.75 89 37 137 101 421.03 5.46 90 37 164 129 340.31 0.57 91 37 165 130 340.28 0.58 92 38 48 10 428.68 7.75 93 38 49 11 428.72 7.75 94 38 137 100 425.51 6.65 95 38 163 127 340.98 0.88 96 38 164 128 340.31 0.57 97 38 193 157 254.02 0.86 98 38 196 160 253.97 0.86 99 38 197 161 253.95 0.86 100 39 47 8 429.06 7.83 101 39 48 9 428.72 7.77 102 39 137 99 428.38 7.62 103 39 148 111 428.61 7.72 104 39 162 125 367.06 0.15 105 39 163 126 351.17 0.24 106 40 46 6 438.81 3.04 107 40 47 7 429.14 7.83 108 40 148 110 429.04 7.84 109 40 161 123 380.35 0.48 110 40 162 124 368.66 0.54 111 40 176 138 333.42 1.88 112 40 180 142 309.42 1.80 113 40 189 151 254.36 0.96 114 40 193 155 254.11 0.90 115 41 45 4 767.71 0.92 116 41 46 5 429.19 7.85 117 41 147 107 429.23 7.87 118 41 14 108 429.19 7.85 119 41 148 109 429.13 7.84 120 41 160 121 384.77 0.95 121 41 161 122 381.17 0.07 122 41 180 141 320.23 3.54 123 42 45 3 814.13 0.90 124 42 184 144 278.36 0.71 125 42 185 145 265.50 5.71 126 42 186 146 257.48 1.79 127 42 189 149 254.89 1.20 128 43 182 141 298.82 5.91 129 43 183 142 295.32 4.19 130 43 184 143 278.41 0.69 131 44 147 104 442.06 4.96 132 44 185 143 278.36 0.71 133 44 186 144 257.80 1.85 134 45 147 103 442.06 4.95 135 45 186 143 257.37 1.73 136 45 215 172 251.72 0.94 137 45 216 173 251.62 0.94 138 46 139 94 442.04 4.95 139 46 147 102 442.23 4.98 140 46 14 103 442.14 4.95 141 46 148 104 442.02 4.95 142 46 186 142 257.04 1.77 143 46 214 170 251.73 0.94 144 46 215 171 251.69 0.94 145 47 189 144 254.74 1.10 146 47 190 145 254.54 1.02 147 47 193 148 254.50 1.05 148 47 212 167 252.15 0.87 149 47 213 168 252.06 0.85 150 47 214 169 251.66 0.95 151 48 67 19 442.21 5.02 152 48 75 27 442.34 5.03 153 48 76 28 442.25 5.00 154 48 77 29 442.10 4.98 155 48 139 92 442.26 4.99 156 48 148 102 442.27 4.99 157 48 212 166 252.09 0.86 158 48 213 167 251.74 0.95 159 49 197 150 253.98 0.86 160 49 211 164 252.12 0.87 161 49 212 165 251.71 0.97 162 50 66 16 442.39 5.00 163 50 67 17 442.41 5.00 164 50 77 27 442.35 5.01 165 50 197 149 253.95 0.86 166 50 210 162 252.12 0.87 167 50 211 163 252.00 0.85 168 51 66 15 442.45 4.99 169 51 202 152 252.80 0.84 170 51 20 160 252.14 0.85 171 51 210 161 252.05 0.86 172 52 61 9 442.49 4.98 173 52 66 14 442.46 4.99 174 52 209 158 252.22 0.83 175 52 20 159 252.10 0.84 176 52 210 160 252.06 0.85 177 52 211 161 252.03 0.85 178 53 203 151 252.74 0.89 179 54 61 7 442.53 4.96 180 56 59 3 788.20 0.49 181 60 63 3 829.22 3.46 182 61 64 3 829.93 4.32 183 61 209 149 252.22 0.83 184 65 85 20 564.03 34.23 185 65 211 148 252.09 0.87 186 65 223 175 120.67 0.37 187 66 77 11 571.43 30.73 188 66 78 12 564.83 34.02 189 66 85 19 577.28 27.62 190 66 211 147 252.09 0.87 191 67 76 9 578.13 27.44 192 67 77 10 576.43 28.23 193 67 85 18 585.82 23.48 194 67 211 146 252.12 0.88 195 67 213 148 251.71 0.97 196 67 221 171 128.19 0.53 197 67 223 173 119.87 0.02 198 68 75 7 592.69 20.23 199 68 76 8 589.16 21.95 200 68 85 17 585.84 23.48 201 68 89 21 590.33 20.93 202 68 93 25 593.72 19.21 203 68 221 170 128.19 0.53 204 69 72 3 867.26 1.82 205 69 74 5 661.87 1.79 206 69 75 6 593.38 19.89 207 69 93 24 593.74 19.21 208 69 221 169 128.18 0.53 209 70 74 4 727.02 3.23 210 70 75 5 635.79 6.56 211 70 76 6 627.96 2.64 212 70 89 19 593.69 19.26 213 70 92 22 593.72 19.21 214 70 93 23 593.72 19.21 215 70 112 43 587.28 15.99 216 70 138 69 556.30 0.55 217 71 74 3 817.27 7.00 218 71 96 25 615.12 8.50 219 71 106 36 614.41 3.04 220 71 107 37 614.03 3.23 221 71 112 42 608.85 5.37 222 72 106 35 614.94 3.02 223 72 219 164 128.19 0.56 224 72 220 165 128.25 0.55 225 72 221 166 128.24 0.55 226 73 140 68 608.17 0.33 227 73 141 69 608.18 0.33 228 73 220 164 128.28 0.54 229 73 221 165 128.20 0.53 230 74 107 34 614.77 2.86 231 74 108 35 614.65 2.80 232 74 109 36 614.28 2.68 233 74 112 39 614.10 2.60 234 74 138 65 608.57 0.31 235 74 139 66 608.49 0.29 236 74 140 67 608.18 0.33 237 75 138 64 608.58 0.30 238 75 139 65 608.50 0.28 239 75 215 142 251.62 0.98 240 75 221 163 128.17 0.53 241 76 85 9 621.53 5.65 242 76 137 62 608.67 0.29 243 76 138 63 608.60 0.29 244 77 85 8 621.52 5.63 245 77 136 60 608.91 0.26 246 77 137 61 608.68 0.32 247 78 81 3 1030.00 0.00 248 78 82 4 851.95 0.88 249 78 85 7 621.53 5.63 250 78 134 57 613.27 1.51 251 78 135 58 610.28 0.07 252 78 136 59 608.91 0.26 253 79 134 56 613.31 1.49 254 79 135 57 610.51 0.11 255 79 165 88 339.94 0.67 256 79 166 89 339.87 0.69 257 80 133 54 615.90 0.55 258 80 134 55 613.34 1.47 259 81 84 3 836.52 3.98 260 81 85 4 818.81 0.39 261 81 130 50 619.18 0.93 262 81 133 53 615.91 0.55 263 81 134 54 615.89 0.54 264 82 85 3 823.31 2.64 265 82 86 4 818.82 0.39 266 83 86 3 818.83 0.39 267 83 87 4 818.83 0.39 268 84 87 3 844.25 1.47 269 84 88 4 818.83 0.38 270 84 130 47 619.20 0.93 271 84 132 49 618.69 0.74 272 84 133 50 618.68 0.74 273 84 134 51 618.58 0.80 274 85 88 3 819.29 0.61 275 85 89 4 812.00 3.04 276 85 134 50 617.31 0.43 277 85 136 52 614.36 1.90 278 86 89 3 821.64 0.56 279 86 90 4 814.38 1.14 280 87 90 3 823.53 4.84 281 87 91 4 811.27 2.69 282 87 132 46 619.13 0.96 283 88 91 3 822.34 0.11 284 88 92 4 805.51 0.93 285 88 116 29 619.58 0.61 286 88 119 32 619.62 0.83 287 88 120 33 619.53 0.79 288 88 132 45 619.12 0.97 289 88 134 47 618.86 1.02 290 88 136 49 614.90 2.94 291 89 92 3 814.89 0.26 292 89 93 4 809.00 2.67 293 90 93 3 817.63 0.27 294 90 94 4 813.70 0.35 295 91 94 3 828.44 3.00 296 91 95 4 813.75 0.33 297 91 119 29 619.72 0.79 298 92 95 3 832.22 3.44 299 92 96 4 816.75 1.83 300 92 112 21 620.50 0.48 301 92 116 25 619.89 0.76 302 93 96 3 830.24 3.73 303 93 97 4 816.75 1.83 304 94 97 3 827.78 3.72 305 94 98 4 817.38 1.48 306 95 98 3 829.54 4.60 307 95 99 4 804.21 8.06 308 95 112 18 620.60 0.52 309 95 115 21 620.39 0.54 310 96 100 4 804.19 8.07 311 96 101 5 789.69 1.86 312 96 107 12 621.83 0.68 313 96 112 17 620.62 0.53 314 97 100 3 829.55 4.61 315 97 101 4 754.36 1.87 316 97 102 5 739.09 5.76 317 97 106 10 623.37 0.77 318 98 102 4 739.09 5.75 319 100 10 3 812.91 1.30 320 100 103 4 764.64 3.62 321 100 105 6 659.13 10.77 322 100 106 7 635.46 6.73 323 100 107 8 623.40 0.76 324 100 108 9 623.47 0.73 325 100 111 12 622.83 0.96 326 101 103 3 829.32 2.65 327 101 104 4 663.40 7.25 328 101 105 5 623.58 0.70 329 101 106 6 623.52 0.71 330 107 112 5 770.13 4.38 331 108 111 3 836.46 0.46 332 108 112 4 808.25 0.80 333 108 142 34 612.60 2.38 334 108 145 37 608.84 0.59 335 109 112 3 826.76 3.72 336 109 113 4 809.06 1.02 337 109 138 29 614.53 3.25 338 109 140 31 614.01 3.04 339 109 141 32 613.32 2.69 340 109 142 33 613.28 2.69 341 109 14 39 608.61 0.48 342 109 149 41 608.46 0.45 343 110 113 3 819.34 0.60 344 110 114 4 809.07 1.02 345 110 145 35 608.94 0.55 346 110 149 40 608.53 0.49 347 111 114 3 809.16 0.99 348 111 115 4 809.13 1.05 349 112 115 3 811.00 1.99 350 112 116 4 815.75 1.62 351 113 116 3 817.00 1.02 352 113 117 4 815.82 1.62 353 113 138 25 614.50 3.16 354 113 149 37 608.40 0.42 355 113 151 39 608.00 0.41 356 113 157 45 492.33 0.40 357 114 117 3 815.90 1.58 358 114 118 4 815.82 1.62 359 114 157 44 493.74 0.28 360 115 118 3 822.58 1.34 361 115 119 4 819.49 0.21 362 116 119 3 822.72 1.81 363 116 120 4 819.50 0.21 364 116 136 20 614.90 3.19 365 116 138 22 614.63 3.06 366 117 120 3 824.44 2.25 367 117 121 4 819.28 0.32 368 117 151 35 608.33 0.39 369 117 155 39 525.46 1.70 370 117 157 41 507.52 7.16 371 118 121 3 820.74 0.40 372 118 122 4 819.29 0.32 373 119 122 3 830.19 2.04 374 119 123 4 819.21 0.36 375 119 132 13 619.60 1.22 376 120 123 3 818.33 0.11 377 120 124 4 818.28 0.09 378 120 132 12 619.48 1.17 379 120 134 14 619.47 1.17 380 120 136 16 618.74 1.24 381 120 151 32 608.38 0.39 382 120 152 33 608.34 0.39 383 120 153 34 607.87 0.40 384 120 155 36 569.18 0.38 385 121 124 3 823.27 2.58 386 121 125 4 818.27 0.08 387 121 154 34 607.87 0.40 388 121 155 35 592.32 1.46 389 122 125 3 819.54 0.56 390 122 126 4 775.81 5.10 391 123 129 6 620.09 1.29 392 123 130 7 619.85 1.26 393 123 131 8 619.72 1.29 394 123 132 9 619.74 1.28 395 124 131 7 619.78 1.26 396 126 129 3 864.96 1.66 397 130 133 3 1030.00 0.00 398 132 153 22 607.98 0.38 399 134 153 20 607.94 0.39 400 135 151 17 608.03 0.40 401 135 152 18 607.94 0.43 402 135 153 19 607.92 0.43 403 135 165 31 339.94 0.66 404 136 149 14 608.35 0.39 405 136 150 15 608.03 0.40 406 136 151 16 608.03 0.39 407 137 148 12 608.35 0.39 408 137 149 13 608.08 0.41 409 137 150 14 608.03 0.40 410 137 163 27 340.56 0.68 411 137 165 29 340.27 0.58 412 138 148 11 608.20 0.34 413 138 149 12 608.06 0.40 414 138 151 14 608.01 0.39 415 139 14 9 608.46 0.46 416 139 148 10 608.05 0.40 417 139 215 77 251.65 0.96 418 140 147 7 608.61 0.49 419 140 14 8 608.57 0.47 420 140 215 76 251.71 0.95 421 141 146 5 608.70 0.53 422 141 147 6 608.62 0.49 423 141 215 75 251.72 0.94 424 141 220 95 128.31 0.54 425 142 145 3 810.43 0.17 426 142 146 4 659.72 10.94 427 147 215 69 251.72 0.95 428 147 216 70 251.72 0.95 429 148 159 11 489.19 1.38 430 148 160 12 486.38 2.62 431 148 162 14 424.14 18.84 432 149 157 8 492.30 0.39 433 149 158 9 491.25 0.72 434 149 159 10 490.62 0.66 435 150 157 7 492.30 0.39 436 150 158 8 491.43 0.81 437 150 160 10 489.21 1.38 438 150 165 15 339.96 0.68 439 151 156 5 577.52 26.15 440 151 157 6 492.29 0.39 441 152 155 3 809.31 5.54 442 152 156 4 595.32 33.23 443 152 158 6 593.50 34.12 444 160 163 3 861.75 0.83 445 161 180 19 308.24 1.23 446 162 176 14 333.25 1.94 447 162 180 18 308.25 1.23 448 163 173 10 333.55 1.83 449 163 176 13 332.81 2.15 450 164 169 5 339.61 0.81 451 164 172 8 334.23 2.17 452 164 173 9 333.50 1.86 453 164 176 12 332.82 2.15 454 165 169 4 337.51 1.68 455 166 169 3 808.83 4.21 456 168 171 3 821.30 4.27 457 168 172 4 807.98 1.14 458 169 172 3 808.91 1.60 459 169 173 4 807.98 1.15 460 170 173 3 813.89 0.26 461 170 174 4 808.24 1.02 462 171 174 3 822.03 1.22 463 171 175 4 814.53 2.12 464 172 175 3 821.62 1.42 465 172 176 4 814.50 2.15 466 172 196 24 253.23 0.83 467 173 176 3 824.90 0.21 468 173 177 4 822.30 1.08 469 174 177 3 824.22 0.55 470 174 178 4 815.16 2.49 471 175 178 3 822.58 1.22 472 175 179 4 812.99 3.59 473 175 195 20 253.80 0.81 474 175 196 21 253.35 0.88 475 176 179 3 822.59 1.21 476 176 180 4 799.56 2.88 477 176 192 16 254.03 0.86 478 176 196 20 253.25 0.82 479 179 192 13 254.05 0.87 480 179 195 16 254.05 0.86 481 180 184 4 822.38 1.01 482 181 184 3 848.18 2.21 483 184 188 4 790.21 4.89 484 185 188 3 828.21 1.71 485 185 189 4 816.96 3.92 486 186 189 3 816.96 3.92 487 186 190 4 816.96 3.92 488 186 214 28 252.16 0.88 489 186 216 30 251.69 0.93 490 187 190 3 816.96 3.92 491 187 191 4 816.96 3.92 492 187 232 60 94.02 1.31 493 188 191 3 816.97 3.92 494 188 192 4 816.95 3.93 495 189 192 3 812.31 1.58 496 189 193 4 809.93 2.69 497 190 193 3 813.79 0.84 498 190 194 4 810.20 2.63 499 190 212 22 252.25 0.86 500 190 214 24 252.12 0.87 501 190 232 57 93.09 1.58 502 191 194 3 815.74 0.07 503 191 195 4 807.96 3.75 504 191 235 59 87.14 2.43 505 191 236 60 47.08 8.86 506 192 195 3 817.06 0.80 507 192 196 4 815.78 0.09 508 193 196 3 817.32 0.67 509 193 197 4 821.02 2.53 510 193 212 19 252.15 0.87 511 194 197 3 821.05 2.52 512 194 198 4 821.02 2.53 513 194 206 11 253.49 0.46 514 194 210 16 252.27 0.87 515 194 212 18 252.14 0.86 516 194 229 50 97.24 0.55 517 194 232 53 92.85 1.47 518 194 233 54 92.12 1.77 519 194 236 57 44.02 7.37 520 195 198 3 826.62 0.27 521 195 199 4 823.16 1.47 522 195 236 56 44.07 7.40 523 196 199 3 836.92 0.10 524 197 200 3 830.88 0.62 525 197 202 4 808.01 3.91 526 197 206 8 253.40 0.41 527 197 210 13 252.16 0.86 528 198 202 3 820.76 2.62 529 198 206 7 253.28 0.35 530 198 236 53 54.52 2.17 531 202 205 3 898.21 5.41 532 202 206 4 807.23 4.44 533 205 20 5 665.22 17.37 534 206 20 4 744.28 7.29 535 206 210 5 645.37 7.44 536 206 233 43 92.84 1.41 537 207 20 3 823.12 0.70 538 207 210 4 742.17 14.81 539 207 229 38 97.27 0.55 540 207 230 39 97.23 0.55 541 207 233 42 92.88 1.41 542 208 226 34 97.31 0.54 543 208 229 37 97.25 0.55 544 208 230 38 97.23 0.55 545 208 233 41 92.89 1.41 546 209 226 33 97.31 0.54 547 210 229 34 97.25 0.55 548 210 233 38 92.88 1.42 549 211 223 27 120.08 0.01 550 212 223 26 119.96 0.05 551 212 224 27 119.77 0.01 552 213 221 23 128.19 0.53 553 213 222 24 127.92 0.61 554 213 223 25 119.95 0.06 555 213 224 26 119.88 0.01 556 214 220 21 128.30 0.53 557 214 221 22 128.19 0.53 558 214 222 23 128.15 0.53 559 214 224 25 123.45 0.57 560 215 220 20 128.27 0.54 561 215 221 21 128.19 0.53 562 216 220 19 128.31 0.54 563 217 220 18 128.32 0.53 564 21 21 14 128.95 0.40 565 21 218 15 128.72 0.46 566 21 219 16 128.60 0.47 567 21 220 17 128.50 0.49 568 21 21 3 812.98 5.61 569 21 21 4 797.47 0.75 570 21 21 13 129.18 0.41 571 21 218 14 128.80 0.46 572 21 219 15 128.75 0.45 573 21 222 18 128.46 0.50 574 21 21 3 807.45 2.83 575 21 21 12 129.16 0.41 576 21 218 13 129.08 0.41 577 21 219 12 129.16 0.42 578 21 222 15 128.95 0.41 579 21 21 8 129.27 0.40 580 21 218 10 129.19 0.41 581 21 219 11 129.19 0.41 582 21 21 3 1029.97 0.01 583 21 21 6 402.95 132.45 584 21 21 3 821.76 3.70 585 21 21 3 877.10 0.10 586 222 228 6 798.15 1.29 587 224 228 4 797.99 1.38 588 224 229 5 790.59 4.06 589 224 232 8 787.06 5.74 590 225 228 3 811.68 2.14 591 225 229 4 803.38 2.00 592 226 229 3 819.67 1.44 593 226 230 4 810.04 1.31 594 227 230 3 827.76 1.72 595 227 231 4 808.67 0.44 596 228 231 3 826.61 5.01 597 228 232 4 814.48 2.46 598 229 232 3 822.21 2.81 599 229 233 4 808.69 0.44 600 230 233 3 808.69 0.44 601 230 234 4 808.69 0.44 602 231 234 3 831.69 2.17 603 232 235 3 813.13 1.84 604 232 236 4 794.88 6.43 605 233 236 3 796.74 7.61