BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 672 675 3 845.11 2.58 2 672 676 4 834.87 2.54 3 673 676 3 850.31 0.03 4 673 677 4 834.88 2.54 5 673 723 50 27.03 0.39 6 673 806 133 24.80 0.30 7 673 834 161 24.63 0.29 8 673 837 164 24.62 0.29 9 674 677 3 843.32 1.69 10 674 678 4 835.07 2.44 11 674 802 128 26.84 1.32 12 674 841 167 24.84 0.32 13 675 678 3 848.52 0.92 14 675 679 4 826.52 1.84 15 676 679 3 829.83 0.20 16 676 680 4 826.52 1.85 17 676 721 45 36.97 0.23 18 676 723 47 36.43 0.34 19 677 680 3 862.10 4.04 20 677 681 4 825.22 2.50 21 677 722 45 36.88 0.20 22 677 723 46 36.88 0.20 23 677 805 128 34.27 1.35 24 677 806 129 34.27 1.35 25 677 809 132 34.98 1.01 26 678 681 3 835.29 2.56 27 678 802 124 35.05 1.02 28 678 805 127 35.06 1.02 29 680 718 38 36.99 0.23 30 680 721 41 37.00 0.23 31 680 722 42 36.99 0.23 32 681 718 37 37.25 0.30 33 681 805 124 36.90 0.20 34 681 808 127 36.93 0.20 35 682 714 32 39.20 0.34 36 682 718 36 37.30 0.29 37 684 766 82 84.73 1.08 38 685 707 22 84.85 1.12 39 685 710 25 84.54 1.28 40 685 711 26 77.38 1.41 41 685 714 29 77.38 1.41 42 685 765 80 84.73 1.08 43 685 766 81 84.90 1.15 44 686 707 21 84.88 1.13 45 686 710 24 84.88 1.13 46 686 765 79 84.80 1.10 47 686 766 80 84.90 1.15 48 686 767 81 85.06 1.07 49 687 703 16 84.95 1.15 50 687 706 19 84.94 1.14 51 687 707 20 84.89 1.12 52 687 710 23 84.92 1.13 53 687 765 78 84.91 1.15 54 687 767 80 85.22 1.01 55 687 768 81 85.22 1.01 56 688 710 22 85.27 1.02 57 688 767 79 85.33 1.03 58 689 699 10 85.41 1.00 59 689 700 11 85.33 1.03 60 689 703 14 85.28 1.02 61 689 767 78 85.34 1.03 62 689 768 79 85.34 1.03 63 690 693 3 854.94 1.66 64 691 767 76 85.46 1.02 65 691 774 83 94.26 3.08 66 695 700 5 815.29 6.86 67 695 773 78 129.56 0.24 68 695 774 79 128.00 0.35 69 695 777 82 129.81 0.28 70 696 699 3 861.30 1.11 71 696 700 4 830.82 0.10 72 696 777 81 129.90 0.26 73 697 700 3 853.96 4.79 74 697 701 4 830.82 0.10 75 697 777 80 129.99 0.22 76 697 778 81 129.91 0.26 77 697 779 82 129.93 0.27 78 697 880 183 147.22 0.51 79 697 887 190 149.78 0.81 80 697 889 192 149.77 0.82 81 698 701 3 846.71 0.80 82 698 702 4 830.01 0.49 83 698 887 189 149.78 0.81 84 698 888 190 149.78 0.81 85 698 889 191 149.75 0.81 86 699 702 3 846.33 0.98 87 699 703 4 836.69 3.83 88 700 703 3 846.97 0.67 89 700 704 4 836.26 4.04 90 700 768 68 149.88 0.83 91 700 770 70 149.89 0.83 92 700 774 74 149.91 0.82 93 700 777 77 149.87 0.79 94 700 778 78 149.81 0.79 95 701 704 3 827.53 0.30 96 701 705 4 827.99 0.54 97 701 778 77 149.92 0.81 98 701 780 79 149.91 0.81 99 701 876 175 149.85 0.83 100 701 880 179 149.90 0.81 101 701 889 188 150.35 0.72 102 702 705 3 828.09 0.58 103 702 706 4 827.99 0.54 104 702 890 188 150.47 0.68 105 703 706 3 827.97 0.55 106 703 707 4 822.80 0.25 107 703 768 65 151.03 0.83 108 704 707 3 823.90 1.48 109 704 708 4 819.46 0.70 110 704 764 60 150.94 0.79 111 704 768 64 153.15 0.01 112 704 770 66 153.16 0.01 113 704 780 76 152.89 0.15 114 705 708 3 831.75 2.63 115 705 709 4 832.38 2.31 116 705 889 184 166.08 0.14 117 705 890 185 166.09 0.14 118 705 891 186 165.81 0.06 119 705 892 187 166.03 0.14 120 705 895 190 165.34 0.23 121 706 709 3 833.39 2.81 122 706 710 4 833.39 2.81 123 706 892 186 166.50 0.07 124 707 710 3 848.39 2.31 125 707 711 4 833.39 2.81 126 707 764 57 165.80 0.07 127 707 765 58 165.88 0.09 128 707 768 61 166.02 0.14 129 708 711 3 855.14 1.26 130 708 712 4 845.84 3.41 131 708 741 33 165.80 0.06 132 708 745 37 165.81 0.06 133 708 895 187 167.64 0.02 134 709 712 3 849.74 1.47 135 709 713 4 845.84 3.42 136 709 741 32 166.72 0.17 137 709 892 183 168.22 0.07 138 709 894 185 168.76 0.34 139 709 895 186 167.85 0.01 140 710 713 3 856.94 0.24 141 710 714 4 845.84 3.42 142 711 714 3 845.83 3.42 143 711 715 4 843.32 4.67 144 711 745 34 167.62 0.03 145 711 752 41 167.83 0.01 146 712 715 3 839.42 2.19 147 712 716 4 829.69 2.14 148 712 737 25 168.89 0.29 149 712 741 29 168.84 0.30 150 712 894 182 169.38 0.23 151 712 898 186 169.08 0.27 152 713 716 3 847.66 2.50 153 713 717 4 829.68 2.13 154 714 717 3 830.63 1.72 155 714 718 4 818.38 2.64 156 715 718 3 833.18 3.00 157 715 719 4 818.31 2.60 158 715 737 22 169.62 0.28 159 715 744 29 169.18 0.26 160 716 719 3 843.88 1.79 161 716 720 4 819.89 3.42 162 716 737 21 172.66 1.22 163 717 720 3 847.13 0.12 164 717 721 4 819.89 3.42 165 718 721 3 829.65 1.23 166 718 722 4 819.88 3.44 167 718 744 26 183.15 0.64 168 718 808 90 183.15 0.64 169 718 809 91 183.15 0.64 170 718 812 94 184.49 1.30 171 719 722 3 833.49 3.39 172 719 725 6 721.16 19.66 173 719 737 18 184.57 1.27 174 719 812 93 183.10 0.66 175 720 725 5 697.96 8.04 176 720 726 6 476.60 11.02 177 720 733 13 186.82 1.23 178 722 725 3 840.83 7.58 179 722 809 87 186.92 1.18 180 722 812 90 186.97 1.16 181 722 813 91 262.15 36.43 182 722 834 112 262.57 36.65 183 723 830 107 262.71 36.66 184 723 834 111 262.67 36.66 185 724 727 3 848.89 1.07 186 724 830 106 274.49 42.55 187 725 728 3 844.39 0.82 188 725 733 8 552.30 96.33 189 725 737 12 406.88 35.43 190 725 812 87 262.83 36.59 191 725 813 88 262.74 36.65 192 725 830 105 274.55 42.56 193 726 733 7 755.46 5.23 194 727 826 99 290.89 50.01 195 728 732 4 823.02 1.79 196 728 733 5 798.27 1.73 197 728 813 85 290.96 50.00 198 728 826 98 292.70 50.91 199 728 827 99 290.92 50.02 200 728 830 102 290.92 50.02 201 729 732 3 839.84 2.87 202 729 733 4 823.05 1.78 203 729 822 93 309.39 49.50 204 729 823 94 307.00 49.99 205 730 733 3 850.18 2.31 206 730 734 4 835.47 5.05 207 731 734 3 855.49 0.35 208 731 735 4 825.99 0.31 209 731 822 91 321.97 43.80 210 732 735 3 845.77 3.78 211 732 736 4 822.96 1.80 212 732 821 89 321.89 43.79 213 732 822 90 322.93 43.32 214 732 823 91 322.93 43.32 215 733 736 3 844.24 4.55 216 733 737 4 839.88 6.73 217 734 737 3 837.31 4.61 218 734 738 4 836.50 5.00 219 734 902 168 255.24 0.33 220 735 738 3 837.31 4.61 221 735 739 4 836.50 5.00 222 735 902 167 254.96 0.46 223 736 739 3 859.14 6.30 224 736 740 4 836.48 5.02 225 736 812 76 328.68 41.04 226 736 821 85 332.31 39.29 227 736 827 91 328.75 40.80 228 737 740 3 853.05 5.80 229 737 741 4 825.33 0.48 230 737 812 75 332.30 39.20 231 738 741 3 832.80 4.21 232 738 898 160 275.46 1.61 233 738 902 164 265.00 1.47 234 739 815 76 335.42 39.77 235 739 817 78 335.95 39.86 236 739 902 163 265.04 1.47 237 739 906 167 258.61 1.72 238 739 911 172 255.37 0.27 239 740 743 3 834.64 5.91 240 740 744 4 820.01 0.93 241 740 811 71 335.32 39.72 242 740 812 72 335.31 39.72 243 740 815 75 335.58 39.69 244 741 744 3 847.04 4.62 245 741 745 4 820.12 0.94 246 741 895 154 291.74 0.40 247 741 898 157 290.13 0.83 248 742 745 3 850.06 3.10 249 742 746 4 820.14 0.96 250 742 870 128 339.67 31.54 251 742 873 131 335.82 25.82 252 742 874 132 331.48 27.41 253 742 877 135 324.69 16.35 254 743 746 3 838.22 1.35 255 743 747 4 828.78 3.36 256 743 811 68 346.94 35.21 257 743 866 123 340.87 32.20 258 743 870 127 339.72 31.55 259 744 747 3 846.24 1.10 260 744 748 4 828.79 3.35 261 744 808 64 346.97 35.19 262 744 811 67 346.99 35.20 263 744 812 68 347.03 35.22 264 745 748 3 840.16 5.91 265 745 749 4 821.98 1.82 266 746 749 3 839.20 0.89 267 746 750 4 822.01 1.83 268 746 787 41 469.04 87.51 269 746 790 44 469.00 87.53 270 746 866 120 348.93 28.19 271 746 869 123 348.82 27.74 272 746 873 127 341.50 24.06 273 747 750 3 847.39 4.99 274 747 751 4 822.75 1.48 275 747 790 43 469.05 87.50 276 747 794 47 469.04 87.50 277 747 804 57 355.98 31.07 278 747 807 60 355.79 31.07 279 747 808 61 355.67 31.13 280 747 811 64 355.61 31.10 281 747 863 116 349.54 28.09 282 747 866 119 349.34 27.98 283 748 751 3 844.05 0.62 284 748 752 4 821.50 2.10 285 748 804 56 355.97 31.07 286 748 808 60 355.69 31.14 287 749 752 3 824.09 2.14 288 749 753 4 821.50 2.10 289 749 762 13 472.60 86.41 290 749 763 14 472.57 86.42 291 749 764 15 472.53 86.42 292 749 787 38 470.75 86.75 293 750 753 3 842.40 0.21 294 750 754 4 813.60 6.05 295 750 790 40 471.06 86.50 296 750 791 41 471.06 86.50 297 750 794 44 471.29 86.38 298 751 754 3 836.20 5.25 299 751 755 4 819.95 5.71 300 751 794 43 471.29 86.38 301 751 799 48 359.48 30.45 302 751 800 49 359.53 30.42 303 751 801 50 359.48 30.44 304 751 804 53 358.40 29.91 305 752 755 3 841.84 0.07 306 752 756 4 795.47 1.91 307 752 763 11 472.62 86.41 308 752 764 12 472.63 86.41 309 753 757 4 748.72 3.06 310 753 758 5 703.22 19.70 311 753 759 6 581.70 32.12 312 753 761 8 564.75 40.46 313 753 762 9 472.62 86.41 314 753 763 10 472.62 86.41 315 753 791 38 471.54 86.42 316 754 758 4 602.55 21.71 317 754 759 5 581.70 32.12 318 754 791 37 471.55 86.42 319 754 794 40 471.43 86.36 320 754 795 41 471.40 86.34 321 756 763 7 564.75 40.45 322 757 760 3 836.18 3.93 323 757 761 4 734.86 20.65 324 757 763 6 564.76 40.45 325 759 788 29 471.67 86.42 326 759 791 32 471.65 86.41 327 759 792 33 471.63 86.39 328 761 769 8 564.54 40.44 329 761 770 9 472.59 86.39 330 761 771 10 472.49 86.39 331 762 769 7 564.53 40.43 332 762 770 8 472.77 86.30 333 762 775 13 472.56 86.34 334 762 784 22 471.73 86.47 335 762 787 25 471.64 86.42 336 762 788 26 471.62 86.41 337 763 766 3 889.21 3.02 338 763 769 6 564.52 40.43 339 764 768 4 830.48 6.24 340 764 770 6 613.21 16.24 341 764 787 23 471.64 86.42 342 765 768 3 830.49 6.23 343 765 769 4 830.49 6.23 344 766 769 3 866.53 2.67 345 768 774 6 818.61 4.63 346 769 774 5 825.46 8.06 347 770 774 4 818.62 4.64 348 770 775 5 818.60 4.63 349 770 778 8 763.24 0.46 350 770 784 14 472.50 86.08 351 771 774 3 842.63 0.52 352 771 775 4 827.66 6.96 353 772 775 3 846.40 2.40 354 772 776 4 830.49 5.57 355 772 781 9 629.01 8.48 356 773 776 3 849.11 1.06 357 773 777 4 846.42 2.40 358 774 777 3 830.50 5.57 359 774 778 4 825.33 8.15 360 775 778 3 845.91 1.44 361 775 779 4 828.97 6.33 362 775 780 5 776.22 5.62 363 775 781 6 628.99 8.48 364 775 784 9 592.02 26.34 365 776 779 3 838.16 5.31 366 776 781 5 706.41 16.11 367 778 876 98 405.29 0.83 368 779 876 97 405.46 0.88 369 779 887 108 364.23 21.97 370 780 783 3 841.07 6.24 371 780 784 4 821.66 2.43 372 780 873 93 405.61 0.68 373 780 876 96 405.25 0.98 374 781 784 3 835.24 0.78 375 781 785 4 823.48 3.33 376 782 785 3 836.67 3.26 377 782 786 4 826.29 1.93 378 782 872 90 406.07 0.73 379 783 786 3 830.73 0.29 380 783 787 4 826.80 4.99 381 783 872 89 405.97 0.79 382 783 873 90 405.91 0.83 383 783 876 93 405.51 1.31 384 784 787 3 846.66 1.14 385 784 788 4 838.12 5.42 386 785 788 3 838.12 5.41 387 785 789 4 835.97 4.33 388 786 789 3 846.59 1.18 389 786 790 4 835.98 4.33 390 786 868 82 406.68 0.50 391 786 869 83 406.56 0.56 392 786 872 86 406.45 0.84 393 787 790 3 835.98 4.33 394 787 791 4 835.98 4.33 395 787 869 82 406.58 0.58 396 787 873 86 406.19 0.98 397 788 791 3 837.33 3.67 398 788 792 4 830.84 6.88 399 789 792 3 854.32 2.71 400 789 793 4 824.25 0.99 401 789 796 7 815.16 0.67 402 790 793 3 854.32 2.71 403 790 794 4 837.97 5.46 404 790 862 72 406.81 0.42 405 790 869 79 406.94 0.70 406 791 794 3 843.61 8.29 407 792 795 3 837.97 5.46 408 792 796 4 819.22 2.80 409 793 796 3 841.70 7.33 410 793 797 4 807.57 4.45 411 793 862 69 407.00 0.42 412 794 798 4 816.35 0.05 413 794 799 5 670.12 0.35 414 794 862 68 407.25 0.55 415 795 798 3 800.61 7.92 416 797 855 58 434.05 13.67 417 797 858 61 431.44 12.42 418 797 862 65 429.37 11.54 419 798 855 57 434.22 13.73 420 799 803 4 817.77 2.35 421 799 804 5 799.45 4.14 422 799 807 8 761.88 12.53 423 799 855 56 434.16 13.77 424 799 859 60 433.82 13.91 425 799 862 63 433.78 13.80 426 800 803 3 844.51 1.31 427 800 804 4 835.21 5.97 428 801 804 3 840.61 3.27 429 801 805 4 837.29 4.93 430 802 805 3 835.86 4.54 431 802 806 4 823.77 0.24 432 802 841 39 440.94 11.69 433 803 806 3 842.37 0.36 434 803 807 4 823.77 0.24 435 803 841 38 440.94 11.69 436 803 855 52 435.31 14.45 437 803 859 56 435.06 14.55 438 804 807 3 851.53 4.22 439 804 808 4 837.25 4.92 440 805 808 3 848.19 5.91 441 805 809 4 837.26 4.92 442 806 809 3 853.85 3.07 443 806 810 4 837.26 4.92 444 806 837 31 441.29 11.66 445 806 838 32 441.26 11.67 446 806 841 35 441.24 11.67 447 807 810 3 853.85 3.06 448 807 811 4 835.17 5.96 449 807 838 31 441.33 11.63 450 807 859 52 434.99 14.63 451 807 863 56 434.70 14.53 452 808 811 3 840.80 4.61 453 808 812 4 835.83 3.61 454 809 812 3 852.94 1.51 455 809 813 4 842.27 6.83 456 809 834 25 441.83 11.47 457 810 813 3 847.11 4.42 458 810 814 4 842.26 6.83 459 810 831 21 442.42 11.39 460 810 834 24 441.67 11.55 461 810 835 25 441.67 11.54 462 810 838 28 441.64 11.54 463 810 863 53 434.78 14.58 464 810 916 106 368.17 37.27 465 811 814 3 842.26 6.83 466 811 863 52 434.75 14.59 467 811 866 55 434.28 14.51 468 811 867 56 433.81 14.71 469 813 827 14 442.57 11.40 470 813 830 17 442.55 11.39 471 813 831 18 442.53 11.40 472 813 834 21 442.53 11.40 473 813 914 101 369.51 37.28 474 814 831 17 442.56 11.39 475 814 913 99 369.58 37.31 476 814 914 100 369.42 37.31 477 815 906 91 375.15 36.76 478 815 911 96 371.17 37.50 479 815 912 97 370.70 37.35 480 815 913 98 369.57 37.32 481 815 914 99 369.57 37.33 482 816 824 8 445.54 12.24 483 816 827 11 443.61 11.69 484 816 911 95 371.48 37.35 485 816 912 96 370.97 37.41 486 816 914 98 370.40 37.48 487 817 820 3 828.61 4.92 488 817 821 4 789.94 7.54 489 817 910 93 373.28 36.48 490 817 911 94 371.95 37.12 491 818 909 91 374.20 36.29 492 818 910 92 373.41 36.45 493 818 912 94 373.12 36.54 494 820 824 4 781.82 0.32 495 821 824 3 812.26 10.32 496 821 827 6 766.72 5.69 497 823 826 3 860.43 1.72 498 823 827 4 832.31 0.36 499 824 827 3 838.46 1.76 500 824 828 4 832.31 0.36 501 825 828 3 837.14 2.57 502 825 829 4 827.99 2.50 503 826 829 3 842.46 0.10 504 826 830 4 827.99 2.50 505 827 830 3 845.05 1.39 506 827 831 4 830.33 0.84 507 827 914 87 370.56 37.46 508 828 831 3 846.97 0.51 509 828 832 4 839.97 3.98 510 829 832 3 845.55 1.22 511 829 833 4 839.99 3.98 512 830 833 3 845.52 1.21 513 830 834 4 834.48 6.73 514 831 834 3 840.80 3.71 515 831 835 4 832.77 7.57 516 831 914 83 369.64 37.28 517 831 916 85 369.41 37.31 518 832 835 3 840.80 3.71 519 832 836 4 832.75 7.55 520 833 836 3 834.70 6.76 521 833 837 4 832.93 7.65 522 834 837 3 842.80 2.60 523 834 838 4 832.25 2.67 524 835 838 3 836.08 0.75 525 835 839 4 837.47 0.06 526 835 856 21 438.02 15.94 527 835 916 81 367.56 36.91 528 836 839 3 843.04 5.36 529 836 840 4 842.99 5.39 530 837 840 3 843.67 5.05 531 837 841 4 842.98 5.40 532 838 841 3 853.11 0.33 533 838 842 4 834.77 1.31 534 838 856 18 438.06 15.96 535 838 859 21 437.85 16.02 536 838 863 25 437.75 16.04 537 839 842 3 846.35 3.71 538 839 843 4 824.12 6.64 539 839 856 17 438.03 15.94 540 840 843 3 834.07 7.47 541 840 844 4 817.22 3.19 542 841 844 3 834.21 3.32 543 842 846 4 817.10 5.24 544 842 852 10 438.37 15.96 545 842 855 13 438.34 15.97 546 842 856 14 438.31 15.96 547 842 859 17 438.13 15.96 548 843 846 3 830.57 1.50 549 843 852 9 438.38 15.96 550 846 851 5 712.83 19.91 551 846 852 6 553.75 1.95 552 846 855 9 497.49 12.35 553 848 851 3 816.31 0.54 554 848 852 4 819.80 7.58 555 849 852 3 834.41 0.29 556 849 853 4 819.64 7.66 557 850 853 3 834.42 0.28 558 850 854 4 826.39 4.29 559 851 854 3 859.11 0.02 560 851 855 4 832.47 1.25 561 852 855 3 837.36 3.91 562 852 856 4 823.66 3.16 563 853 856 3 832.74 0.54 564 853 857 4 825.76 2.10 565 854 857 3 836.06 0.71 566 854 858 4 825.81 2.08 567 855 858 3 855.33 2.69 568 855 859 4 828.14 3.25 569 856 859 3 855.33 2.69 570 856 860 4 818.33 5.79 571 856 916 60 365.05 36.88 572 857 860 3 855.03 2.84 573 857 861 4 818.28 5.80 574 857 917 60 364.88 36.83 575 858 861 3 830.76 5.17 576 858 862 4 822.22 6.06 577 859 862 3 841.27 2.35 578 859 863 4 835.77 0.71 579 860 863 3 837.80 1.71 580 860 864 4 839.66 1.22 581 860 916 56 364.88 36.83 582 860 917 57 364.88 36.83 583 861 864 3 848.98 0.43 584 861 865 4 839.66 1.21 585 861 917 56 364.87 36.83 586 862 865 3 845.07 1.93 587 862 866 4 844.27 2.33 588 863 866 3 855.55 0.76 589 863 916 53 365.47 37.12 590 864 867 3 867.33 5.10 591 864 868 4 857.60 0.27 592 864 915 51 365.50 37.13 593 864 916 52 365.50 37.13 594 864 917 53 365.46 37.12 595 865 868 3 872.85 2.35 596 865 869 4 855.94 1.11 597 866 869 3 855.94 1.11 598 866 870 4 833.57 1.75 599 867 870 3 853.26 2.45 600 867 871 4 838.30 1.28 601 867 913 46 368.73 37.67 602 867 915 48 367.79 38.07 603 868 871 3 842.23 2.83 604 868 872 4 838.70 1.08 605 868 915 47 367.83 38.09 606 869 872 3 834.78 0.89 607 869 873 4 834.00 0.51 608 870 873 3 843.86 5.42 609 870 874 4 833.78 0.39 610 871 874 3 834.05 0.52 611 871 875 4 818.69 7.16 612 871 906 35 375.75 36.56 613 871 907 36 375.71 36.58 614 872 875 3 861.51 3.32 615 872 876 4 818.68 7.15 616 873 876 3 843.58 6.38 617 873 877 4 832.15 1.52 618 874 877 3 845.40 4.63 619 874 878 4 825.16 5.01 620 874 903 29 376.72 37.05 621 874 906 32 376.63 36.99 622 874 907 33 375.86 36.61 623 875 878 3 845.41 4.62 624 875 879 4 832.15 1.52 625 875 907 32 375.86 36.60 626 876 879 3 837.61 2.49 627 876 880 4 826.11 4.55 628 877 880 3 851.31 8.00 629 877 881 4 826.11 4.55 630 877 891 14 379.94 36.48 631 877 899 22 377.18 37.02 632 878 881 3 848.23 5.89 633 878 882 4 829.69 2.81 634 878 899 21 377.16 36.99 635 878 903 25 377.12 37.01 636 878 904 26 377.11 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