BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 672 675 3 835.95 3.08 2 672 676 4 834.87 2.54 3 673 676 3 850.31 0.03 4 673 677 4 834.88 2.54 5 673 723 50 28.95 0.27 6 673 806 133 25.50 0.09 7 673 834 161 25.32 0.01 8 673 837 164 25.50 0.09 9 674 677 3 848.52 0.92 10 674 678 4 825.32 2.25 11 674 802 128 27.53 0.98 12 674 806 132 25.55 0.12 13 674 841 167 25.68 0.05 14 675 678 3 860.27 4.96 15 675 679 4 819.67 5.25 16 676 679 3 826.49 1.84 17 676 680 4 819.67 5.25 18 676 723 47 32.84 0.86 19 677 680 3 862.10 4.04 20 677 681 4 819.66 5.25 21 677 722 45 33.15 0.83 22 677 723 46 33.15 0.83 23 677 805 128 31.59 0.26 24 677 806 129 31.60 0.26 25 677 809 132 31.85 0.39 26 678 681 3 835.29 2.56 27 678 805 127 31.96 0.45 28 680 718 38 33.86 1.05 29 680 721 41 33.86 1.05 30 680 722 42 33.82 1.07 31 681 718 37 36.41 0.01 32 681 804 123 33.38 0.83 33 681 805 124 33.34 0.84 34 681 808 127 33.79 1.02 35 682 714 32 43.06 2.27 36 682 718 36 36.61 0.07 37 684 766 82 83.83 0.66 38 685 707 22 83.94 0.67 39 685 710 25 83.54 0.77 40 685 711 26 77.42 1.43 41 685 714 29 78.39 0.94 42 685 765 80 83.85 0.65 43 685 766 81 83.97 0.68 44 686 707 21 83.98 0.69 45 686 710 24 83.98 0.69 46 686 765 79 83.92 0.67 47 686 766 80 84.00 0.68 48 686 767 81 84.01 0.68 49 687 703 16 84.24 0.79 50 687 706 19 84.23 0.79 51 687 707 20 83.98 0.69 52 687 710 23 84.01 0.68 53 687 765 78 84.00 0.68 54 687 767 80 84.33 0.56 55 687 768 81 84.16 0.62 56 688 710 22 84.56 0.67 57 688 767 79 84.63 0.69 58 689 699 10 84.71 0.65 59 689 700 11 84.63 0.69 60 689 703 14 84.61 0.69 61 689 767 78 84.66 0.69 62 689 768 79 84.62 0.68 63 690 693 3 856.47 2.42 64 691 767 76 84.73 0.66 65 691 774 83 89.31 0.60 66 695 700 5 805.78 7.41 67 695 773 78 129.56 0.24 68 695 774 79 127.84 0.43 69 695 777 82 129.72 0.32 70 696 699 3 849.87 6.83 71 696 700 4 819.94 4.01 72 696 777 81 129.84 0.28 73 697 700 3 854.33 4.61 74 697 701 4 827.27 0.88 75 697 777 80 129.90 0.26 76 697 778 81 129.85 0.29 77 697 779 82 129.85 0.29 78 697 880 183 147.16 0.48 79 697 887 190 149.77 0.82 80 697 889 192 149.77 0.82 81 698 701 3 846.32 0.98 82 698 702 4 820.50 4.26 83 698 887 189 149.77 0.82 84 698 888 190 149.79 0.82 85 698 889 191 149.77 0.82 86 699 702 3 846.33 0.98 87 699 703 4 820.50 4.26 88 700 703 3 846.05 0.21 89 700 704 4 827.97 0.53 90 700 768 68 149.88 0.81 91 700 770 70 149.86 0.79 92 700 774 74 149.85 0.79 93 700 777 77 149.87 0.79 94 700 778 78 149.85 0.79 95 701 704 3 835.38 3.59 96 701 705 4 827.56 0.32 97 701 778 77 149.93 0.81 98 701 780 79 149.88 0.79 99 701 876 175 149.90 0.80 100 701 880 179 149.91 0.81 101 701 889 188 150.46 0.67 102 702 705 3 846.25 3.08 103 702 706 4 827.56 0.32 104 702 890 188 150.56 0.63 105 703 706 3 827.56 0.32 106 703 707 4 818.94 4.62 107 703 768 65 150.91 0.77 108 704 707 3 838.62 5.24 109 704 708 4 815.84 4.08 110 704 764 60 150.90 0.77 111 704 768 64 152.03 0.28 112 704 770 66 151.26 0.94 113 704 780 76 150.88 0.75 114 705 708 3 825.69 1.69 115 705 709 4 824.48 1.01 116 705 889 184 165.57 0.67 117 705 890 185 165.48 0.61 118 705 891 186 165.08 0.53 119 705 892 187 165.25 0.53 120 705 895 190 164.28 0.23 121 706 709 3 832.37 2.31 122 706 710 4 832.36 2.31 123 706 892 186 165.71 0.68 124 707 710 3 848.39 2.31 125 707 711 4 833.94 3.39 126 707 764 57 165.04 0.61 127 707 765 58 165.15 0.57 128 707 768 61 165.20 0.56 129 708 711 3 846.68 2.98 130 708 712 4 833.95 3.39 131 708 741 33 165.15 0.58 132 708 745 37 165.13 0.57 133 708 895 187 167.52 0.96 134 709 712 3 849.72 1.46 135 709 713 4 846.68 2.98 136 709 892 183 167.78 0.96 137 709 894 185 167.78 0.96 138 709 895 186 167.63 0.92 139 710 713 3 849.72 1.46 140 710 714 4 846.68 2.98 141 711 714 3 848.46 2.33 142 711 715 4 833.06 2.98 143 711 745 34 167.72 0.96 144 711 752 41 167.80 0.94 145 712 715 3 848.45 2.32 146 712 716 4 829.45 1.14 147 712 737 25 170.12 0.20 148 712 741 29 167.82 0.95 149 712 894 182 174.79 0.17 150 712 898 186 174.47 0.09 151 713 716 3 837.72 2.98 152 713 717 4 829.97 0.86 153 714 717 3 834.82 4.44 154 714 718 4 820.68 2.64 155 715 718 3 832.44 3.24 156 715 719 4 821.09 2.84 157 715 737 22 175.36 0.12 158 715 741 26 174.80 0.04 159 715 744 29 174.80 0.04 160 715 745 30 175.16 0.23 161 716 719 3 843.20 2.14 162 716 720 4 819.93 3.41 163 716 734 18 187.32 0.18 164 716 737 21 180.33 2.52 165 717 720 3 836.96 4.97 166 717 721 4 819.95 3.42 167 718 721 3 837.66 5.24 168 718 722 4 819.94 3.41 169 718 744 26 187.94 0.02 170 718 808 90 189.14 0.58 171 718 809 91 187.94 0.02 172 718 812 94 189.25 0.64 173 719 722 3 833.69 3.29 174 719 725 6 611.77 35.04 175 719 737 18 189.36 0.61 176 719 812 93 189.25 0.64 177 720 725 5 618.34 31.75 178 720 726 6 539.62 1.04 179 720 733 13 191.62 1.01 180 720 734 14 191.66 0.98 181 720 737 17 191.62 1.01 182 722 725 3 836.90 5.62 183 722 809 87 191.66 0.98 184 722 812 90 191.68 0.98 185 722 813 91 261.40 35.84 186 722 834 112 262.30 36.30 187 723 830 107 262.38 36.33 188 723 834 111 262.31 36.30 189 724 727 3 848.89 1.07 190 724 830 106 262.35 36.31 191 725 728 3 844.39 0.82 192 725 733 8 519.53 6.77 193 725 737 12 412.29 36.26 194 725 812 87 262.25 36.22 195 725 830 105 342.28 1.25 196 726 733 7 726.28 3.81 197 728 732 4 815.73 2.00 198 728 733 5 795.03 0.21 199 728 813 85 378.73 0.92 200 728 826 98 388.34 3.16 201 728 827 99 385.25 4.16 202 728 830 102 385.25 4.16 203 729 732 3 850.18 2.31 204 729 733 4 832.32 6.62 205 729 822 93 390.72 2.50 206 729 823 94 390.22 2.25 207 730 733 3 850.18 2.31 208 730 734 4 835.35 5.11 209 731 734 3 855.49 0.35 210 731 735 4 822.91 1.72 211 731 822 91 397.12 1.06 212 732 735 3 845.76 3.77 213 732 736 4 822.93 1.73 214 732 821 89 397.11 1.06 215 732 822 90 397.14 1.07 216 732 823 91 398.42 1.69 217 733 736 3 844.22 4.55 218 733 737 4 836.47 5.02 219 734 737 3 836.48 5.02 220 734 738 4 836.48 5.02 221 735 738 3 836.49 5.01 222 735 739 4 836.47 5.02 223 735 902 167 260.35 0.57 224 736 739 3 859.14 6.30 225 736 740 4 836.47 5.02 226 736 812 76 402.34 1.53 227 736 821 85 409.95 0.57 228 736 827 91 409.96 0.58 229 737 740 3 853.05 5.80 230 737 741 4 802.88 3.77 231 737 812 75 408.89 0.26 232 738 741 3 832.70 4.26 233 738 894 156 288.59 1.54 234 738 898 160 265.69 1.31 235 738 902 164 265.44 1.34 236 739 815 76 412.67 0.76 237 739 817 78 412.68 0.74 238 739 902 163 265.64 1.29 239 739 905 166 265.41 1.36 240 739 906 167 265.25 1.39 241 739 911 172 264.26 1.11 242 740 743 3 849.11 1.32 243 740 744 4 814.64 0.60 244 740 811 71 412.40 0.95 245 740 812 72 417.05 1.38 246 740 815 75 412.73 0.79 247 741 744 3 850.08 3.10 248 741 745 4 818.87 1.52 249 741 895 154 295.78 1.39 250 741 898 157 289.94 0.88 251 742 745 3 851.63 2.32 252 742 746 4 820.01 0.94 253 742 870 128 409.04 0.30 254 742 873 131 396.12 2.71 255 742 874 132 390.47 1.29 256 742 877 135 362.42 1.15 257 743 746 3 844.79 1.10 258 743 747 4 817.03 0.56 259 743 811 68 432.34 0.40 260 743 866 123 409.25 0.35 261 743 870 127 409.06 0.30 262 744 747 3 835.91 4.07 263 744 748 4 817.99 4.90 264 744 808 64 432.56 0.36 265 744 811 67 432.36 0.40 266 744 812 68 432.38 0.39 267 745 748 3 835.43 3.82 268 745 749 4 813.16 1.39 269 746 749 3 832.30 2.56 270 746 750 4 814.55 2.08 271 746 787 41 490.12 11.71 272 746 790 44 490.13 11.71 273 746 866 120 432.12 0.52 274 746 869 123 427.76 0.63 275 746 870 124 427.62 0.70 276 746 873 127 420.64 0.79 277 747 750 3 847.24 5.07 278 747 751 4 815.41 1.66 279 747 790 43 490.22 11.71 280 747 794 47 490.13 11.71 281 747 804 57 433.53 0.39 282 747 807 60 433.37 0.40 283 747 808 61 433.37 0.40 284 747 811 64 433.27 0.42 285 747 862 115 432.94 0.29 286 747 863 116 432.91 0.32 287 747 866 119 432.13 0.53 288 748 751 3 835.13 0.24 289 748 752 4 830.57 2.52 290 748 804 56 433.42 0.39 291 748 808 60 433.26 0.42 292 749 752 3 830.76 2.43 293 749 753 4 828.79 3.42 294 749 762 13 494.47 10.96 295 749 763 14 494.48 10.96 296 749 764 15 494.43 10.97 297 749 787 38 490.64 11.79 298 750 753 3 839.23 1.80 299 750 754 4 828.80 3.42 300 750 791 41 490.30 11.65 301 750 794 44 490.30 11.65 302 751 754 3 834.33 6.18 303 751 755 4 828.77 3.41 304 751 794 43 490.29 11.66 305 751 799 48 439.56 2.56 306 751 800 49 435.61 0.57 307 751 801 50 435.56 0.55 308 751 804 53 433.42 0.39 309 752 755 3 841.84 0.07 310 752 756 4 820.55 7.52 311 752 763 11 494.54 10.97 312 752 764 12 495.00 10.80 313 753 756 3 846.32 1.19 314 753 757 4 805.27 5.23 315 753 758 5 783.44 4.07 316 753 759 6 518.37 0.47 317 753 761 8 503.17 6.92 318 753 762 9 496.45 9.98 319 753 763 10 494.51 10.96 320 753 791 38 490.63 11.80 321 754 758 4 606.78 17.97 322 754 759 5 520.78 1.67 323 754 791 37 490.63 11.80 324 754 794 40 490.61 11.80 325 754 795 41 490.61 11.81 326 756 763 7 500.95 8.68 327 757 760 3 823.21 1.12 328 757 761 4 789.71 1.52 329 757 763 6 515.82 1.74 330 759 788 29 491.08 11.78 331 759 791 32 490.94 11.83 332 759 792 33 490.73 11.85 333 761 769 8 500.76 8.46 334 761 770 9 500.26 8.30 335 761 771 10 495.10 10.88 336 762 769 7 500.79 8.47 337 762 770 8 500.31 8.27 338 762 775 13 500.31 8.27 339 762 784 22 491.24 11.84 340 762 787 25 491.16 11.81 341 762 788 26 490.99 11.72 342 763 766 3 889.21 3.02 343 763 769 6 504.12 10.14 344 764 768 4 804.61 6.71 345 764 770 6 573.49 14.71 346 764 784 20 491.25 11.82 347 764 787 23 491.19 11.79 348 765 768 3 830.48 6.23 349 765 769 4 813.48 2.27 350 766 769 3 866.53 2.67 351 768 774 6 804.06 1.23 352 769 774 5 805.32 1.86 353 770 774 4 804.05 1.23 354 770 775 5 796.48 2.56 355 770 778 8 778.45 6.43 356 770 784 14 502.13 6.67 357 771 774 3 842.62 0.53 358 771 775 4 825.38 8.12 359 772 775 3 837.78 1.91 360 772 776 4 827.88 6.87 361 772 781 9 576.34 17.89 362 772 788 16 502.15 6.68 363 773 776 3 849.10 1.06 364 773 777 4 827.88 6.88 365 774 777 3 829.76 5.94 366 774 778 4 825.41 8.11 367 775 778 3 842.34 0.35 368 775 779 4 825.43 8.10 369 775 780 5 763.02 12.28 370 775 781 6 576.25 17.93 371 775 784 9 525.76 5.30 372 775 788 13 565.44 23.21 373 776 779 3 846.25 2.33 374 776 781 5 692.46 19.78 375 778 876 98 429.38 0.10 376 779 876 97 429.39 0.12 377 779 887 108 314.07 0.85 378 780 783 3 847.53 3.00 379 780 784 4 818.66 2.61 380 780 873 93 431.01 0.63 381 780 876 96 428.22 0.09 382 781 784 3 831.89 0.90 383 781 785 4 829.00 7.76 384 782 785 3 843.87 0.34 385 782 786 4 833.50 5.53 386 783 786 3 843.24 0.67 387 783 787 4 830.57 7.04 388 783 872 89 431.02 0.63 389 783 873 90 430.99 0.64 390 783 876 93 428.19 0.07 391 784 787 3 846.66 1.14 392 784 788 4 835.97 4.33 393 785 788 3 838.12 5.41 394 785 789 4 835.97 4.33 395 786 789 3 835.98 4.33 396 786 790 4 835.97 4.33 397 786 868 82 432.66 0.45 398 786 869 83 432.31 0.59 399 786 872 86 431.60 0.64 400 787 790 3 835.98 4.34 401 787 791 4 835.95 4.33 402 787 869 82 432.14 0.66 403 787 873 86 430.99 0.64 404 788 791 3 837.22 3.62 405 788 792 4 821.84 2.45 406 789 792 3 843.24 8.25 407 789 793 4 822.15 2.03 408 789 796 7 803.57 6.63 409 790 793 3 825.27 0.48 410 790 794 4 835.58 6.66 411 790 862 72 433.33 0.42 412 790 866 76 432.88 0.42 413 790 869 79 432.61 0.46 414 791 794 3 848.69 5.75 415 792 795 3 836.61 6.15 416 792 796 4 822.06 2.60 417 793 796 3 835.10 10.63 418 793 797 4 819.78 3.74 419 793 862 69 433.56 0.46 420 794 798 4 774.14 8.40 421 794 799 5 728.38 31.29 422 794 804 10 490.00 26.64 423 794 862 68 433.39 0.40 424 795 798 3 818.57 1.89 425 797 855 58 438.51 0.38 426 797 858 61 435.49 0.52 427 797 862 65 435.42 0.48 428 798 855 57 438.76 0.38 429 799 803 4 824.64 1.09 430 799 804 5 823.06 1.89 431 799 807 8 774.73 3.26 432 799 855 56 438.78 0.38 433 799 859 60 438.64 0.41 434 799 862 63 438.59 0.42 435 800 803 3 834.75 3.19 436 800 804 4 824.65 1.09 437 801 804 3 830.46 1.80 438 801 805 4 827.63 0.39 439 802 805 3 843.01 0.96 440 802 806 4 827.70 0.43 441 802 841 39 442.13 0.31 442 803 806 3 844.70 1.53 443 803 807 4 827.70 0.43 444 803 841 38 442.16 0.30 445 803 855 52 441.88 0.29 446 803 859 56 441.44 0.36 447 804 807 3 854.71 3.48 448 804 808 4 837.83 4.62 449 805 808 3 848.03 6.82 450 805 809 4 837.85 4.62 451 806 809 3 837.86 4.62 452 806 810 4 837.87 4.62 453 806 838 32 442.22 0.29 454 806 841 35 442.25 0.31 455 807 810 3 853.85 3.06 456 807 811 4 837.98 4.68 457 807 838 31 442.19 0.29 458 807 859 52 441.81 0.32 459 807 862 55 441.76 0.32 460 807 863 56 441.38 0.15 461 808 811 3 855.48 2.73 462 808 812 4 844.36 7.81 463 809 812 3 860.12 0.07 464 809 813 4 852.93 1.50 465 809 834 25 442.70 0.15 466 810 813 3 852.93 1.51 467 810 814 4 842.33 6.80 468 810 831 21 442.88 0.16 469 810 834 24 442.70 0.15 470 810 835 25 442.69 0.15 471 810 838 28 442.63 0.18 472 810 863 53 441.80 0.34 473 810 916 106 295.32 0.88 474 811 814 3 841.87 7.04 475 811 863 52 441.80 0.34 476 811 866 55 441.66 0.31 477 811 867 56 441.45 0.35 478 813 827 14 443.07 0.18 479 813 830 17 443.06 0.18 480 813 831 18 443.01 0.18 481 813 834 21 443.01 0.18 482 813 914 101 295.93 0.94 483 814 831 17 443.04 0.18 484 814 867 53 441.62 0.40 485 814 913 99 295.94 0.93 486 814 914 100 295.72 0.83 487 815 906 91 298.50 1.55 488 815 911 96 296.89 0.97 489 815 912 97 296.46 0.95 490 815 913 98 295.94 0.92 491 815 914 99 295.94 0.92 492 816 821 5 588.09 19.57 493 816 824 8 475.30 14.80 494 816 827 11 443.60 0.07 495 816 911 95 296.89 0.97 496 816 912 96 296.75 1.03 497 816 914 98 296.28 0.99 498 817 820 3 817.71 0.54 499 817 821 4 795.26 11.76 500 817 910 93 297.07 0.91 501 817 911 94 296.89 0.96 502 818 909 91 298.62 1.49 503 818 910 92 297.69 1.21 504 818 912 94 297.02 0.96 505 820 824 4 777.86 1.66 506 821 824 3 834.56 0.83 507 821 827 6 754.62 1.39 508 823 826 3 852.26 2.37 509 823 827 4 832.26 0.35 510 824 827 3 832.26 0.34 511 824 828 4 830.93 1.01 512 825 828 3 832.27 0.35 513 825 829 4 830.94 1.01 514 826 829 3 830.97 1.01 515 826 830 4 840.39 3.71 516 827 830 3 840.39 3.71 517 827 831 4 832.54 7.66 518 827 914 87 296.59 0.96 519 828 831 3 839.95 3.97 520 828 832 4 836.55 5.66 521 829 832 3 843.13 2.38 522 829 833 4 832.59 7.65 523 830 833 3 849.15 0.65 524 830 834 4 843.05 2.40 525 831 834 3 843.24 2.49 526 831 835 4 822.12 2.39 527 831 914 83 296.26 0.85 528 831 916 85 295.95 0.95 529 832 835 3 843.24 2.49 530 832 836 4 832.57 2.83 531 833 836 3 843.24 2.49 532 833 837 4 843.24 2.49 533 834 837 3 829.50 1.30 534 834 838 4 829.50 1.30 535 835 838 3 837.29 1.19 536 835 839 4 827.99 2.09 537 835 856 21 443.17 0.73 538 835 916 81 295.85 0.94 539 836 839 3 842.96 5.40 540 836 840 4 842.98 5.40 541 837 840 3 842.99 5.40 542 837 841 4 842.99 5.39 543 838 841 3 846.33 3.73 544 838 842 4 834.81 1.30 545 838 856 18 443.17 0.72 546 838 859 21 443.12 0.70 547 838 863 25 442.78 0.84 548 839 842 3 837.86 0.23 549 839 843 4 834.78 1.30 550 839 856 17 443.18 0.73 551 840 843 3 834.06 7.48 552 840 844 4 828.25 4.57 553 841 844 3 855.27 3.13 554 842 846 4 833.58 7.23 555 842 852 10 443.81 0.48 556 842 855 13 443.35 0.69 557 842 856 14 443.36 0.69 558 842 859 17 443.35 0.69 559 843 846 3 840.82 3.63 560 843 852 9 443.81 0.48 561 846 851 5 770.89 4.81 562 846 852 6 656.12 5.51 563 846 855 9 609.93 1.85 564 848 851 3 836.72 0.67 565 848 852 4 820.38 4.58 566 849 852 3 836.45 0.54 567 849 853 4 799.95 3.37 568 850 853 3 835.17 0.10 569 850 854 4 810.97 2.38 570 851 854 3 838.20 1.41 571 851 855 4 812.02 2.93 572 852 855 3 822.80 1.35 573 852 856 4 827.10 3.52 574 853 856 3 832.89 0.61 575 853 857 4 827.11 3.50 576 854 857 3 833.15 0.74 577 854 858 4 831.68 1.23 578 855 858 3 848.59 6.06 579 855 859 4 828.14 3.25 580 856 859 3 855.33 2.69 581 856 860 4 828.13 3.25 582 856 916 60 293.98 1.21 583 857 860 3 864.34 7.49 584 857 861 4 820.03 0.21 585 857 917 60 293.56 1.18 586 858 861 3 830.80 5.16 587 858 862 4 819.95 0.29 588 859 862 3 833.36 1.62 589 859 863 4 829.68 3.35 590 860 863 3 832.14 2.12 591 860 864 4 839.62 1.23 592 860 916 56 293.49 1.20 593 860 917 57 293.49 1.20 594 861 864 3 847.16 5.00 595 861 865 4 845.46 4.09 596 861 917 56 293.46 1.21 597 862 865 3 849.19 3.99 598 862 866 4 847.44 3.10 599 863 866 3 858.75 0.85 600 863 916 53 293.48 1.21 601 864 867 3 867.34 5.10 602 864 868 4 858.76 0.84 603 864 915 51 293.49 1.21 604 864 916 52 293.48 1.21 605 864 917 53 293.44 1.20 606 865 868 3 872.38 2.58 607 865 869 4 855.94 1.11 608 866 869 3 857.65 0.26 609 866 870 4 855.93 1.11 610 867 870 3 855.93 1.11 611 867 871 4 847.27 3.20 612 867 913 46 294.08 1.16 613 867 915 48 293.48 1.21 614 868 871 3 842.44 2.73 615 868 872 4 838.91 0.97 616 868 915 47 293.49 1.21 617 869 872 3 835.34 0.80 618 869 873 4 834.79 0.89 619 870 873 3 835.34 0.81 620 870 874 4 834.77 0.88 621 871 874 3 834.37 0.67 622 871 875 4 833.74 0.36 623 871 906 35 299.14 1.27 624 871 907 36 299.12 1.26 625 872 875 3 861.51 3.32 626 872 876 4 819.95 8.25 627 873 876 3 846.39 4.97 628 873 877 4 825.76 5.34 629 874 877 3 836.31 0.08 630 874 878 4 833.32 1.57 631 874 903 29 299.15 1.26 632 874 906 32 299.16 1.26 633 874 907 33 299.13 1.27 634 875 878 3 836.32 0.08 635 875 879 4 833.36 1.56 636 875 907 32 299.15 1.27 637 876 879 3 834.55 0.96 638 876 880 4 833.36 1.55 639 877 880 3 851.31 8.00 640 877 881 4 827.40 1.43 641 877 891 14 301.64 1.25 642 877 899 22 299.72 1.14 643 878 881 3 833.22 1.05 644 878 882 4 833.21 1.05 645 878 899 21 299.89 1.23 646 878 903 25 299.55 1.22 647 878 904 26 299.51 1.23 648 878 907 29 299.29 1.25 649 879 882 3 833.22 1.05 650 879 883 4 833.21 1.05 651 880 883 3 837.26 0.99 652 880 884 4 817.91 6.59 653 880 887 7 720.54 11.49 654 880 889 9 395.87 47.41 655 880 891 11 302.55 0.85 656 881 884 3 827.16 2.43 657 881 891 10 301.69 1.26 658 881 896 15 300.81 1.02 659 881 899 18 300.76 1.00 660 881 900 19 300.74 0.99 661 882 885 3 942.49 0.04 662 883 886 3 837.83 0.21 663 883 887 4 835.44 0.95 664 884 887 3 838.03 0.35 665 884 888 4 833.83 0.15 666 884 889 5 480.76 15.64 667 884 891 7 406.56 52.75 668 891 895 4 827.98 5.57 669 891 896 5 777.49 5.82 670 891 899 8 720.45 10.13 671 892 895 3 837.57 0.78 672 892 896 4 819.35 1.23 673 893 896 3 850.97 6.70 674 893 897 4 834.39 2.37 675 894 897 3 835.88 1.62 676 894 898 4 834.38 2.36 677 895 898 3 834.88 2.11 678 895 899 4 830.60 2.71 679 896 899 3 833.37 1.35 680 896 900 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