BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 114 112 29.44 1.02 2 2 136 134 29.64 1.12 3 2 138 136 30.33 1.31 4 2 141 139 30.32 1.30 5 3 134 131 30.35 1.30 6 3 135 132 30.65 1.46 7 3 136 133 30.33 1.30 8 3 137 134 30.36 1.32 9 3 138 135 30.64 1.46 10 3 168 165 30.83 1.51 11 5 9 4 811.70 1.74 12 5 10 5 445.89 39.35 13 5 13 8 433.92 33.36 14 5 135 130 34.61 0.03 15 5 136 131 35.27 0.37 16 6 10 4 810.18 1.79 17 7 10 3 833.53 2.45 18 7 11 4 823.02 2.81 19 8 11 3 825.54 1.54 20 8 12 4 824.64 4.73 21 8 57 49 43.24 0.19 22 8 88 80 43.31 0.17 23 8 112 104 46.86 0.45 24 8 184 176 43.32 0.13 25 8 185 177 43.24 0.18 26 8 187 179 43.23 0.18 27 9 12 3 829.41 2.69 28 9 13 4 825.84 4.13 29 9 112 103 44.86 0.55 30 9 136 127 46.16 0.95 31 9 184 175 43.32 0.13 32 10 13 3 825.90 4.11 33 10 14 4 825.90 4.11 34 11 14 3 828.51 3.12 35 11 15 4 817.19 4.40 36 11 87 76 51.77 0.84 37 11 88 77 51.55 0.74 38 12 15 3 819.52 3.24 39 12 16 4 839.28 6.65 40 12 85 73 51.56 0.75 41 12 88 76 51.55 0.74 42 12 112 100 51.54 0.75 43 13 16 3 839.29 6.65 44 13 17 4 839.28 6.65 45 13 114 101 57.99 0.46 46 13 136 123 52.08 0.93 47 14 17 3 841.92 5.33 48 14 18 4 839.28 6.65 49 15 18 3 833.25 0.50 50 15 19 4 821.61 0.40 51 15 85 70 61.32 1.12 52 15 87 72 61.33 1.12 53 16 19 3 817.95 2.16 54 16 112 96 61.30 1.11 55 16 114 98 61.67 1.10 56 16 115 99 69.45 2.74 57 16 118 102 61.70 1.12 58 17 114 97 69.41 2.76 59 17 115 98 69.44 2.75 60 18 115 97 74.72 3.87 61 19 84 65 71.92 2.44 62 19 85 66 71.20 2.08 63 19 93 74 75.44 4.19 64 19 111 92 74.54 3.77 65 19 115 96 74.77 3.87 66 21 117 96 75.95 4.21 67 21 121 100 86.54 1.08 68 22 93 71 77.25 4.01 69 22 94 72 87.22 0.79 70 22 97 75 87.28 0.82 71 22 121 99 86.58 1.07 72 22 125 103 87.11 0.91 73 24 124 100 112.67 0.07 74 24 125 101 107.31 1.62 75 25 124 99 115.32 0.53 76 26 29 3 945.20 0.97 77 26 30 4 888.19 0.85 78 26 123 97 137.22 0.29 79 26 124 98 135.81 0.82 80 26 126 100 127.84 4.79 81 26 127 101 140.66 1.44 82 26 128 102 143.80 0.59 83 28 100 72 143.84 0.62 84 28 103 75 144.02 0.54 85 28 104 76 144.06 0.55 86 28 105 77 146.90 0.23 87 28 107 79 147.97 1.06 88 28 126 98 144.01 0.54 89 28 128 100 149.00 0.82 90 29 128 99 149.31 0.95 91 30 123 93 152.28 2.40 92 30 128 98 149.02 0.81 93 30 152 122 160.09 1.01 94 30 153 123 160.69 0.70 95 30 154 124 160.00 1.01 96 31 104 73 154.69 1.60 97 31 106 75 149.10 0.84 98 31 128 97 159.71 0.91 99 32 35 3 824.75 1.86 100 32 36 4 815.28 3.33 101 33 36 3 830.00 0.76 102 33 37 4 816.05 2.96 103 33 49 16 159.97 1.00 104 33 78 45 160.02 1.01 105 33 79 46 159.97 1.01 106 33 106 73 160.01 1.01 107 33 108 75 160.03 1.01 108 33 129 96 160.69 0.68 109 33 176 143 167.80 0.98 110 34 37 3 826.82 2.35 111 34 38 4 816.16 3.02 112 34 155 121 167.84 1.01 113 34 176 142 167.13 0.66 114 34 206 172 167.66 0.88 115 35 38 3 822.09 0.06 116 35 39 4 826.62 2.31 117 36 39 3 826.72 2.26 118 36 40 4 826.61 2.31 119 36 77 41 168.00 0.99 120 36 78 42 167.98 0.99 121 36 105 69 167.97 0.99 122 36 106 70 167.91 1.02 123 37 40 3 826.73 2.26 124 37 41 4 826.49 2.38 125 37 47 10 168.00 0.99 126 37 49 12 168.00 0.99 127 37 78 41 168.02 0.98 128 37 176 139 168.58 0.90 129 37 206 169 168.48 0.94 130 37 208 171 168.18 0.94 131 37 228 191 168.12 0.95 132 37 229 192 168.09 0.95 133 38 41 3 826.62 2.31 134 38 42 4 827.12 2.06 135 38 206 168 168.46 0.95 136 39 42 3 828.04 4.24 137 39 43 4 821.28 0.85 138 40 43 3 821.27 0.86 139 40 44 4 769.21 26.89 140 40 45 5 646.00 72.92 141 40 46 6 646.00 72.92 142 40 47 7 168.11 0.97 143 40 78 38 168.12 0.98 144 41 44 3 817.49 1.03 145 41 228 187 168.44 0.95 146 46 219 173 224.30 0.29 147 46 230 184 224.83 0.29 148 46 246 200 122.73 0.35 149 46 247 201 106.73 0.38 150 47 78 31 374.35 58.77 151 47 228 181 224.99 0.27 152 47 229 182 224.86 0.29 153 47 230 183 224.84 0.30 154 48 77 29 374.88 58.55 155 48 78 30 374.88 58.54 156 48 79 31 374.89 58.54 157 48 219 171 224.90 0.28 158 48 230 182 224.87 0.29 159 48 232 184 224.80 0.30 160 48 236 188 212.52 0.30 161 48 243 195 129.31 0.54 162 48 246 198 121.23 1.08 163 48 247 199 111.24 1.88 164 49 78 29 374.91 58.55 165 49 79 30 374.88 58.54 166 49 80 31 374.88 58.53 167 49 81 32 374.91 58.50 168 49 208 159 254.95 9.80 169 49 229 180 225.16 0.29 170 49 230 181 224.91 0.28 171 49 231 182 224.86 0.29 172 49 232 183 224.86 0.29 173 50 73 23 375.30 58.44 174 50 77 27 375.04 58.56 175 50 79 29 374.96 58.54 176 50 80 30 374.92 58.52 177 50 81 31 374.96 58.50 178 50 232 182 224.88 0.29 179 50 236 186 212.55 0.31 180 50 243 193 129.32 0.54 181 51 69 18 375.64 58.42 182 51 81 30 374.97 58.50 183 51 232 181 224.94 0.29 184 51 233 182 224.79 0.29 185 51 237 186 212.55 0.31 186 52 64 12 376.18 58.20 187 52 66 14 375.60 58.40 188 52 69 17 375.64 58.42 189 52 81 29 375.36 58.38 190 52 82 30 375.27 58.34 191 52 83 31 375.31 58.32 192 52 237 185 212.56 0.30 193 53 60 7 376.56 58.13 194 53 83 30 375.39 58.33 195 53 89 36 375.61 58.25 196 53 233 180 225.21 0.30 197 53 237 184 224.09 0.29 198 54 61 7 376.53 58.12 199 54 62 8 376.34 58.20 200 54 63 9 376.29 58.20 201 54 89 35 375.75 58.26 202 54 90 36 375.60 58.33 203 55 60 5 379.06 56.89 204 55 61 6 376.53 58.12 205 55 89 34 375.76 58.26 206 55 90 35 375.75 58.27 207 56 59 3 826.57 2.44 208 56 60 4 392.71 50.09 209 56 88 32 375.88 58.30 210 56 89 33 375.80 58.26 211 57 88 31 375.92 58.31 212 57 89 32 375.92 58.32 213 57 187 130 263.25 10.25 214 60 234 174 225.85 0.53 215 60 237 177 225.12 0.26 216 60 238 178 225.03 0.30 217 62 237 175 224.94 0.30 218 62 238 176 224.91 0.29 219 64 69 5 806.62 1.25 220 64 237 173 224.84 0.31 221 64 240 176 219.81 1.96 222 65 68 3 838.31 2.75 223 65 69 4 808.09 1.99 224 66 69 3 817.40 2.60 225 66 70 4 816.27 2.11 226 66 95 29 422.80 35.11 227 66 99 33 422.92 35.06 228 67 70 3 834.02 0.61 229 67 71 4 816.28 2.11 230 67 98 31 426.00 33.53 231 67 99 32 422.91 35.08 232 67 102 35 426.41 33.66 233 68 71 3 817.82 1.35 234 68 72 4 826.40 1.79 235 69 72 3 837.28 3.55 236 69 73 4 827.95 1.12 237 69 236 167 224.77 0.30 238 69 237 168 223.30 0.48 239 69 240 171 154.11 11.77 240 70 73 3 829.07 0.61 241 70 74 4 827.99 1.10 242 70 80 10 411.60 40.73 243 70 99 29 434.20 29.78 244 70 103 33 455.02 19.59 245 71 74 3 845.83 3.55 246 71 75 4 845.83 3.55 247 71 102 31 473.54 17.42 248 71 103 32 457.60 18.12 249 72 75 3 861.02 4.64 250 72 76 4 845.87 3.54 251 72 240 168 154.11 11.77 252 72 241 169 130.38 0.09 253 72 243 171 129.34 0.52 254 72 244 172 129.27 0.55 255 73 76 3 850.01 1.47 256 73 77 4 821.78 2.02 257 73 80 7 433.32 30.23 258 73 236 163 223.41 0.43 259 73 240 167 130.39 0.09 260 73 243 170 129.29 0.54 261 74 103 29 551.97 0.32 262 74 104 30 573.35 2.21 263 74 105 31 539.65 3.15 264 76 243 167 129.31 0.54 265 76 244 168 129.25 0.55 266 76 247 171 111.23 1.88 267 77 80 3 887.77 1.03 268 77 105 28 598.75 0.18 269 77 243 166 129.30 0.54 270 78 105 27 598.89 0.24 271 78 106 28 598.75 0.18 272 79 105 26 598.89 0.24 273 79 106 27 598.75 0.18 274 80 99 19 599.19 0.23 275 80 103 23 599.19 0.23 276 80 105 25 599.11 0.21 277 80 106 26 599.00 0.23 278 80 107 27 598.70 0.17 279 80 108 28 598.70 0.17 280 81 108 27 598.70 0.17 281 81 110 29 598.61 0.13 282 81 233 152 224.83 0.28 283 82 95 13 599.33 0.21 284 82 96 14 599.14 0.29 285 82 99 17 599.25 0.24 286 82 107 25 599.12 0.22 287 82 108 26 599.00 0.23 288 82 109 27 598.75 0.18 289 82 110 28 598.69 0.17 290 83 89 6 599.67 0.22 291 83 91 8 599.56 0.24 292 83 110 27 598.73 0.18 293 83 233 150 225.35 0.38 294 84 91 7 599.56 0.24 295 84 92 8 599.50 0.22 296 84 93 9 599.49 0.21 297 84 96 12 599.35 0.21 298 84 109 25 599.12 0.21 299 84 110 26 599.01 0.21 300 84 111 27 598.83 0.18 301 85 88 3 881.52 2.20 302 85 89 4 599.74 0.24 303 85 90 5 599.74 0.24 304 85 91 6 599.63 0.23 305 85 111 26 598.90 0.20 306 85 112 27 598.89 0.20 307 86 90 4 599.73 0.24 308 86 91 5 599.70 0.23 309 89 112 23 599.00 0.23 310 92 95 3 841.50 2.81 311 92 96 4 821.03 3.93 312 93 96 3 838.97 1.55 313 93 97 4 810.20 9.35 314 93 109 16 599.16 0.23 315 93 118 25 408.05 2.62 316 93 121 28 348.50 31.90 317 93 125 32 347.49 32.26 318 94 97 3 828.29 0.30 319 94 98 4 821.72 3.60 320 95 98 3 828.23 1.75 321 95 99 4 821.74 3.61 322 96 99 3 835.59 3.92 323 96 100 4 821.74 3.61 324 96 107 11 599.24 0.21 325 96 109 13 599.09 0.18 326 96 125 29 347.16 32.06 327 96 127 31 347.46 31.78 328 97 100 3 828.98 7.23 329 97 101 4 828.98 7.23 330 97 125 28 347.16 32.07 331 98 101 3 847.26 1.92 332 98 102 4 828.97 7.23 333 99 102 3 821.19 3.91 334 99 103 4 805.81 9.43 335 99 107 8 599.25 0.21 336 100 103 3 842.17 0.64 337 100 107 7 599.26 0.22 338 100 125 25 347.82 31.73 339 100 126 26 347.01 32.00 340 103 107 4 853.01 1.36 341 106 128 22 346.88 31.42 342 107 127 20 347.19 31.48 343 107 128 21 346.98 31.40 344 107 129 22 346.89 31.41 345 108 129 21 346.89 31.41 346 108 130 22 346.82 31.44 347 108 131 23 346.79 31.46 348 108 157 49 346.75 31.45 349 109 113 4 888.16 0.12 350 109 118 9 348.81 31.63 351 109 122 13 347.89 31.60 352 109 127 18 347.67 31.61 353 109 129 20 347.03 31.40 354 109 130 21 346.88 31.41 355 112 118 6 713.23 38.00 356 112 133 21 347.88 31.47 357 112 136 24 348.38 31.50 358 112 184 72 347.36 31.21 359 113 118 5 668.01 15.39 360 113 119 6 627.88 88.17 361 113 130 17 348.30 31.43 362 113 131 18 348.45 31.51 363 113 132 19 348.04 31.44 364 113 137 24 348.64 31.44 365 113 145 32 349.72 31.64 366 114 118 4 829.39 2.82 367 114 136 22 348.70 31.47 368 114 137 23 348.69 31.46 369 114 145 31 349.87 31.71 370 115 118 3 848.56 3.87 371 115 145 30 349.88 31.70 372 116 119 3 847.20 3.19 373 116 120 4 826.02 1.14 374 116 145 29 349.94 31.68 375 116 148 32 367.69 22.80 376 117 120 3 819.46 5.81 377 117 121 4 814.59 4.57 378 118 121 3 827.25 10.89 379 118 122 4 809.68 2.12 380 119 122 3 839.93 4.55 381 119 123 4 809.63 2.12 382 119 130 11 370.98 23.77 383 119 145 26 371.62 23.96 384 119 148 29 371.95 24.00 385 119 149 30 371.84 24.00 386 119 152 33 372.03 24.03 387 119 154 35 371.87 23.99 388 120 123 3 827.31 1.76 389 120 124 4 807.21 3.33 390 120 148 28 372.03 24.04 391 120 152 32 372.11 24.07 392 121 124 3 843.07 5.02 393 121 125 4 807.16 3.35 394 122 125 3 841.72 5.70 395 122 126 4 822.10 4.11 396 122 127 5 458.07 27.27 397 122 130 8 371.44 23.99 398 122 154 32 385.84 17.21 399 123 126 3 837.69 3.69 400 123 152 29 392.30 15.46 401 123 154 31 372.03 24.04 402 127 154 27 453.26 27.48 403 128 154 26 453.26 27.48 404 129 154 25 453.26 27.48 405 129 155 26 453.26 27.48 406 129 156 27 453.19 27.50 407 130 156 26 453.29 27.48 408 130 157 27 453.26 27.48 409 131 157 26 453.28 27.48 410 131 159 28 453.24 27.48 411 132 137 5 704.23 44.86 412 132 142 10 454.01 27.39 413 132 145 13 454.06 27.41 414 132 146 14 453.93 27.41 415 132 156 24 453.85 27.41 416 132 157 25 453.81 27.41 417 132 158 26 453.77 27.39 418 132 159 27 453.72 27.37 419 133 136 3 835.23 9.41 420 133 137 4 813.70 8.11 421 133 159 26 453.81 27.41 422 133 184 51 453.80 27.39 423 134 137 3 835.90 3.00 424 134 142 8 454.18 27.42 425 134 158 24 453.91 27.41 426 134 159 25 453.87 27.41 427 134 160 26 453.81 27.42 428 134 165 31 453.83 27.41 429 134 168 34 453.86 27.41 430 135 184 49 453.68 27.50 431 137 141 4 754.82 27.82 432 137 142 5 710.72 37.74 433 137 145 8 698.37 42.30 434 138 141 3 811.11 0.32 435 138 142 4 811.12 0.30 436 139 142 3 811.13 0.31 437 139 143 4 816.50 2.38 438 139 168 29 454.98 27.75 439 139 171 32 455.96 27.75 440 139 172 33 455.92 27.77 441 140 143 3 836.11 4.37 442 140 144 4 808.32 6.48 443 141 144 3 821.56 0.14 444 141 145 4 802.98 3.77 445 142 145 3 823.63 1.18 446 142 146 4 817.56 1.00 447 142 158 16 456.25 27.97 448 142 168 26 456.35 27.93 449 142 172 30 456.42 27.94 450 143 146 3 855.30 5.21 451 143 147 4 817.58 0.99 452 143 172 29 456.38 27.94 453 144 147 3 836.16 4.36 454 144 148 4 817.57 1.00 455 145 148 3 831.35 1.95 456 145 149 4 812.80 1.35 457 146 149 3 833.68 0.79 458 146 150 4 811.10 0.51 459 146 156 10 456.43 27.94 460 146 158 12 456.39 27.95 461 146 172 26 456.49 27.95 462 147 150 3 816.99 3.46 463 147 151 4 811.07 0.51 464 148 151 3 841.25 8.67 465 148 152 4 812.80 1.42 466 149 152 3 843.74 7.42 467 149 153 4 840.17 9.19 468 149 154 5 710.73 38.23 469 149 156 7 567.43 21.84 470 150 153 3 840.18 9.19 471 150 154 4 788.88 1.64 472 150 155 5 632.87 0.68 473 150 156 6 633.23 0.51 474 150 173 23 676.64 1.85 475 150 174 24 638.08 1.83 476 155 175 20 694.76 0.71 477 155 176 21 694.74 0.72 478 156 176 20 694.83 0.71 479 157 176 19 694.86 0.70 480 157 177 20 694.86 0.70 481 157 178 21 694.78 0.73 482 157 208 51 694.76 0.72 483 158 169 11 695.35 0.65 484 158 172 14 695.35 0.65 485 158 174 16 695.17 0.68 486 158 176 18 694.95 0.70 487 158 177 19 694.88 0.69 488 158 178 20 694.88 0.69 489 159 178 19 694.89 0.69 490 159 180 21 694.87 0.70 491 160 165 5 695.58 0.69 492 160 166 6 695.57 0.69 493 160 169 9 695.57 0.69 494 160 177 17 695.24 0.66 495 160 178 18 695.22 0.67 496 160 179 19 695.02 0.73 497 160 180 20 694.91 0.69 498 160 197 37 695.54 0.67 499 160 201 41 695.57 0.69 500 161 165 4 809.94 7.30 501 161 180 19 694.92 0.68 502 161 182 21 694.91 0.68 503 161 184 23 694.93 0.76 504 161 193 32 695.28 0.65 505 161 197 36 695.54 0.68 506 162 165 3 822.26 1.20 507 162 166 4 810.00 7.33 508 162 193 31 695.33 0.68 509 162 197 35 695.54 0.68 510 163 166 3 822.46 2.78 511 163 167 4 810.00 7.33 512 163 197 34 695.54 0.67 513 163 200 37 695.71 0.65 514 164 167 3 822.75 2.94 515 164 168 4 816.39 5.01 516 165 168 3 840.41 5.90 517 165 169 4 820.51 2.06 518 166 169 3 838.43 6.90 519 166 170 4 820.51 2.06 520 166 177 11 695.62 0.62 521 166 197 31 695.60 0.67 522 166 200 34 695.79 0.60 523 166 201 35 695.79 0.61 524 167 170 3 831.84 2.04 525 167 171 4 813.74 1.33 526 168 171 3 822.08 2.83 527 168 172 4 813.70 1.35 528 169 172 3 837.99 5.92 529 169 173 4 813.68 1.34 530 169 174 5 736.89 19.86 531 169 177 8 695.56 0.53 532 170 173 3 821.17 7.34 533 170 177 7 695.88 0.65 534 170 202 32 696.62 0.48 535 170 205 35 719.70 0.83 536 174 177 3 881.58 4.20 537 174 205 31 777.80 1.00 538 175 205 30 777.80 1.00 539 175 206 31 777.78 0.99 540 176 205 29 777.83 0.99 541 176 206 30 777.80 0.99 542 176 208 32 777.78 0.99 543 177 201 24 778.02 0.98 544 177 205 28 777.98 1.00 545 177 206 29 777.86 0.98 546 177 207 30 777.79 0.99 547 177 208 31 777.79 0.99 548 178 208 30 777.79 0.99 549 179 197 18 778.13 0.99 550 179 198 19 778.12 0.99 551 179 201 22 778.12 0.99 552 179 207 28 777.98 1.00 553 179 208 29 777.88 0.99 554 179 209 30 777.79 1.00 555 179 210 31 777.78 0.99 556 180 210 30 777.79 1.00 557 180 211 31 777.78 1.00 558 181 189 8 778.50 0.97 559 181 190 9 778.43 0.96 560 181 191 10 778.26 0.99 561 181 194 13 778.17 0.97 562 181 210 29 777.90 0.99 563 181 211 30 777.79 1.01 564 182 188 6 778.54 0.98 565 182 189 7 778.52 0.98 566 182 190 8 778.50 0.97 567 182 211 29 777.88 0.99 568 183 186 3 859.09 1.22 569 183 187 4 778.75 1.05 570 183 188 5 778.58 0.97 571 183 190 7 778.52 0.98 572 184 187 3 820.41 0.47 573 187 211 24 778.07 1.00 574 188 211 23 778.06 1.01 575 189 211 22 777.95 0.98 576 189 212 23 777.86 0.99 577 191 194 3 821.34 0.01 578 191 211 20 778.01 1.02 579 191 212 21 777.95 1.02 580 191 213 22 777.85 1.02 581 191 218 27 671.58 15.81 582 193 196 3 829.38 4.03 583 193 197 4 800.69 6.51 584 194 197 3 831.86 2.21 585 194 198 4 815.76 0.93 586 194 222 28 263.18 17.94 587 195 198 3 829.30 3.48 588 195 199 4 811.61 1.06 589 195 225 30 228.31 0.53 590 196 199 3 831.99 1.49 591 196 200 4 820.58 0.25 592 197 200 3 831.77 4.44 593 197 201 4 830.37 5.14 594 198 201 3 848.62 1.02 595 198 207 9 778.18 1.05 596 198 222 24 260.14 16.43 597 198 225 27 228.22 0.48 598 198 227 29 228.13 0.51 599 201 205 4 818.05 5.37 600 201 207 6 778.31 1.02 601 202 205 3 837.47 1.54 602 202 207 5 804.17 1.56 603 203 207 4 852.29 2.30 604 206 228 22 227.25 0.64 605 207 226 19 228.03 0.46 606 207 227 20 227.56 0.62 607 207 228 21 227.55 0.63 608 207 229 22 227.27 0.65 609 208 227 19 227.96 0.48 610 208 229 21 227.27 0.65 611 208 231 23 227.25 0.65 612 209 213 4 835.44 1.81 613 209 222 13 228.19 0.42 614 209 227 18 228.04 0.45 615 209 229 20 227.59 0.61 616 209 230 21 227.33 0.66 617 209 231 22 227.31 0.66 618 210 231 21 227.31 0.66 619 212 218 6 793.51 5.51 620 213 218 5 792.97 5.26 621 213 219 6 789.32 4.89 622 213 222 9 789.14 4.98 623 213 230 17 228.28 0.45 624 213 232 19 227.95 0.48 625 213 235 22 227.35 0.65 626 214 218 4 839.62 0.76 627 214 235 21 227.47 0.58 628 214 242 28 134.00 0.38 629 214 246 32 129.34 0.54 630 215 218 3 834.24 3.46 631 215 219 4 831.95 2.96 632 215 246 31 129.33 0.54 633 216 219 3 831.07 3.40 634 216 220 4 829.66 4.10 635 216 245 29 129.33 0.53 636 216 246 30 129.22 0.55 637 216 248 32 128.66 0.76 638 217 220 3 829.68 4.10 639 217 221 4 812.38 4.54 640 218 221 3 836.00 5.54 641 218 222 4 831.53 6.38 642 219 222 3 848.50 2.10 643 219 223 4 799.93 8.62 644 219 230 11 228.40 0.49 645 219 232 13 228.38 0.49 646 219 246 27 128.59 0.81 647 220 223 3 837.76 7.46 648 220 224 4 830.39 4.98 649 221 224 3 848.50 2.10 650 221 225 4 830.39 4.98 651 222 225 3 818.97 0.10 652 222 226 4 812.58 3.29 653 222 227 5 781.80 1.90 654 222 230 8 341.62 35.37 655 223 226 3 828.91 4.61 656 223 227 4 790.32 5.33 657 223 228 5 789.88 5.55 658 223 230 7 657.95 63.82 659 227 230 3 938.62 1.27 660 232 235 3 891.79 6.34 661 232 236 4 820.19 0.26 662 232 246 14 128.43 0.77 663 233 236 3 844.08 3.06 664 233 237 4 820.18 0.26 665 234 237 3 827.87 0.96 666 234 238 4 820.18 0.26 667 235 238 3 827.80 0.29 668 235 239 4 820.15 0.26 669 235 242 7 330.46 49.68 670 235 246 11 252.84 11.54 671 236 239 3 820.36 0.36 672 236 240 4 784.39 2.27 673 236 242 6 259.34 14.12 674 236 243 7 258.68 14.44 675 236 246 10 252.86 11.53 676 239 242 3 842.55 1.80 677 240 243 3 846.62 1.42 678 241 244 3 836.84 0.65 679 242 245 3 811.95 5.07 680 242 246 4 807.71 7.19 681 243 246 3 833.97 5.95 682 243 247 4 812.55 4.76 683 244 247 3 846.10 0.12 684 244 248 4 834.96 1.19 685 245 248 3 839.17 3.35