BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 672 675 3 842.49 3.89 2 672 676 4 840.11 5.12 3 673 676 3 850.31 0.03 4 673 677 4 840.11 5.13 5 673 723 50 26.62 0.09 6 673 806 133 24.60 0.28 7 673 834 161 24.23 0.14 8 673 837 164 24.22 0.14 9 674 677 3 848.52 0.92 10 674 678 4 834.89 2.53 11 674 802 128 24.78 0.35 12 674 841 167 24.78 0.35 13 675 678 3 848.52 0.92 14 675 679 4 823.97 3.10 15 676 679 3 825.20 2.48 16 676 680 4 823.98 3.10 17 676 723 47 30.11 0.99 18 677 680 3 862.10 4.04 19 677 681 4 823.97 3.10 20 677 722 45 30.79 0.66 21 677 723 46 30.89 0.62 22 677 805 128 27.61 0.94 23 677 806 129 27.61 0.94 24 677 809 132 28.08 0.76 25 678 681 3 835.29 2.56 26 678 805 127 28.09 0.78 27 680 718 38 32.50 1.23 28 680 721 41 32.38 1.18 29 680 722 42 32.38 1.18 30 681 805 124 30.97 0.56 31 681 808 127 30.98 0.57 32 682 714 32 43.05 2.27 33 682 718 36 36.43 0.01 34 684 766 82 82.73 0.09 35 685 707 22 82.75 0.07 36 685 710 25 81.92 0.04 37 685 711 26 77.37 1.40 38 685 714 29 77.38 1.40 39 685 765 80 82.68 0.06 40 685 766 81 82.77 0.07 41 686 707 21 82.77 0.07 42 686 710 24 82.77 0.07 43 686 765 79 82.75 0.07 44 686 766 80 82.80 0.06 45 686 767 81 82.85 0.07 46 687 703 16 82.82 0.08 47 687 706 19 82.86 0.09 48 687 707 20 82.77 0.07 49 687 710 23 82.80 0.06 50 687 765 78 82.84 0.08 51 687 767 80 83.05 0.07 52 687 768 81 82.88 0.10 53 688 710 22 83.17 0.06 54 688 767 79 83.25 0.04 55 689 699 10 83.32 0.11 56 689 700 11 83.23 0.06 57 689 703 14 83.22 0.07 58 689 767 78 83.27 0.04 59 689 768 79 83.25 0.04 60 690 693 3 854.94 1.66 61 691 767 76 83.45 0.09 62 691 774 83 94.11 3.16 63 695 700 5 825.75 1.64 64 695 773 78 126.22 0.05 65 695 774 79 126.08 0.04 66 695 777 82 126.31 0.08 67 696 699 3 861.30 1.11 68 696 700 4 826.55 2.04 69 696 777 81 126.33 0.07 70 697 700 3 853.96 4.79 71 697 701 4 826.77 1.93 72 697 777 80 127.62 0.52 73 697 778 81 126.33 0.07 74 697 779 82 128.69 0.03 75 697 880 183 144.53 0.06 76 697 887 190 146.17 0.08 77 697 889 192 146.12 0.06 78 698 701 3 846.34 0.97 79 698 702 4 826.77 1.93 80 698 888 190 146.17 0.07 81 699 702 3 846.32 0.98 82 699 703 4 836.82 3.76 83 700 703 3 846.05 0.21 84 700 704 4 836.82 3.77 85 700 768 68 146.33 0.08 86 700 770 70 146.31 0.09 87 700 774 74 146.33 0.10 88 700 777 77 146.37 0.10 89 700 778 78 146.30 0.10 90 701 704 3 826.13 1.58 91 701 705 4 826.05 1.61 92 701 778 77 146.44 0.14 93 701 876 175 146.28 0.10 94 701 880 179 146.48 0.19 95 701 889 188 146.97 0.37 96 702 705 3 834.73 2.69 97 702 706 4 826.04 1.60 98 702 890 188 147.02 0.34 99 703 706 3 832.00 1.38 100 703 707 4 827.53 0.34 101 703 768 65 147.61 0.36 102 704 707 3 827.53 0.34 103 704 708 4 827.53 0.33 104 704 764 60 147.50 0.40 105 704 768 64 150.08 1.54 106 704 770 66 150.68 1.85 107 704 780 76 150.05 1.57 108 705 708 3 833.20 1.91 109 705 709 4 833.19 1.91 110 705 889 184 164.11 0.82 111 705 890 185 164.12 0.82 112 705 891 186 164.04 0.83 113 705 892 187 164.10 0.82 114 705 895 190 163.40 0.74 115 706 709 3 840.40 1.69 116 706 710 4 832.39 2.31 117 706 892 186 164.34 0.71 118 707 710 3 848.39 2.31 119 707 711 4 833.22 1.90 120 707 764 57 163.84 0.92 121 707 765 58 164.01 0.84 122 707 768 61 164.01 0.85 123 708 711 3 845.82 3.41 124 708 712 4 835.40 1.81 125 708 741 33 163.98 0.84 126 708 745 37 163.84 0.92 127 708 895 187 165.15 0.47 128 709 712 3 849.72 1.46 129 709 713 4 845.83 3.40 130 709 741 32 164.81 0.56 131 709 892 183 165.77 0.70 132 709 894 185 165.83 0.68 133 709 895 186 165.66 0.72 134 710 713 3 847.62 2.50 135 710 714 4 848.31 2.16 136 711 714 3 848.31 2.17 137 711 715 4 833.67 2.68 138 711 745 34 165.10 0.50 139 712 715 3 836.65 3.67 140 712 716 4 826.58 0.87 141 712 737 25 166.65 0.83 142 712 741 29 165.84 0.66 143 712 898 186 167.08 1.04 144 713 716 3 850.66 1.01 145 713 717 4 836.14 3.91 146 714 717 3 834.70 4.63 147 714 718 4 824.22 0.32 148 715 718 3 833.81 2.69 149 715 719 4 824.25 0.29 150 715 737 22 167.66 0.87 151 715 744 29 167.18 1.10 152 716 719 3 842.12 2.68 153 716 720 4 817.93 2.87 154 716 737 21 172.25 1.43 155 717 720 3 841.32 1.26 156 717 721 4 820.80 2.96 157 718 721 3 837.66 5.24 158 718 722 4 821.83 2.46 159 718 744 26 183.38 0.59 160 718 808 90 183.37 0.60 161 718 809 91 183.25 0.54 162 718 812 94 183.31 0.56 163 719 722 3 835.12 2.57 164 719 725 6 622.07 29.91 165 719 737 18 184.55 1.17 166 719 812 93 183.81 0.81 167 720 725 5 721.15 18.28 168 720 726 6 609.71 36.08 169 720 733 13 188.62 0.38 170 722 725 3 836.90 5.62 171 722 809 87 188.78 0.43 172 722 812 90 188.81 0.44 173 722 813 91 261.39 35.85 174 722 834 112 262.83 36.59 175 723 830 107 262.88 36.59 176 723 834 111 262.87 36.58 177 724 727 3 848.89 1.07 178 724 830 106 262.88 36.59 179 725 728 3 846.57 1.33 180 725 733 8 568.36 2.81 181 725 737 12 265.37 37.20 182 725 812 87 263.14 36.44 183 725 813 88 263.09 36.47 184 725 830 105 344.18 0.41 185 726 733 7 744.25 5.11 186 727 826 99 390.54 3.15 187 728 732 4 821.88 0.38 188 728 733 5 790.96 2.13 189 728 813 85 390.12 3.21 190 728 826 98 391.01 2.94 191 728 827 99 390.51 3.14 192 728 830 102 390.54 3.15 193 729 732 3 839.84 2.87 194 729 733 4 815.20 3.71 195 729 822 93 406.78 10.54 196 729 823 94 403.88 9.09 197 730 733 3 850.19 2.31 198 730 734 4 820.85 0.89 199 731 734 3 850.18 2.31 200 731 735 4 817.13 1.54 201 731 822 91 408.10 10.66 202 732 735 3 845.77 3.78 203 732 736 4 817.16 1.57 204 732 821 89 408.00 10.71 205 732 822 90 408.10 10.66 206 732 823 91 408.23 10.72 207 733 736 3 844.23 4.55 208 733 737 4 839.85 6.74 209 734 737 3 837.31 4.60 210 734 738 4 837.31 4.60 211 735 738 3 839.61 3.46 212 735 739 4 837.31 4.60 213 735 902 167 259.19 1.09 214 736 739 3 859.13 6.31 215 736 740 4 819.20 2.64 216 736 812 76 418.59 7.74 217 736 821 85 421.38 6.33 218 736 827 91 421.35 6.33 219 737 740 3 852.94 5.86 220 737 741 4 809.27 7.60 221 737 812 75 421.20 6.41 222 738 741 3 832.70 4.26 223 738 894 156 285.45 0.09 224 738 898 160 266.13 1.04 225 738 902 164 265.92 1.09 226 739 815 76 421.55 6.37 227 739 817 78 422.47 6.73 228 739 902 163 265.94 1.10 229 739 905 166 265.92 1.10 230 739 906 167 265.79 1.12 231 739 911 172 263.94 0.94 232 740 743 3 849.11 1.32 233 740 744 4 818.89 1.50 234 740 811 71 424.12 5.07 235 740 812 72 421.87 6.20 236 740 815 75 421.71 6.45 237 741 744 3 850.08 3.10 238 741 745 4 821.89 1.87 239 741 895 154 292.76 1.02 240 741 898 157 289.82 0.96 241 742 745 3 851.63 2.32 242 742 746 4 820.00 0.94 243 742 870 128 409.50 0.56 244 742 873 131 402.41 0.53 245 742 874 132 389.64 1.67 246 743 746 3 836.31 5.34 247 743 747 4 816.40 2.73 248 743 811 68 435.50 1.69 249 743 866 123 413.14 2.29 250 743 870 127 411.28 1.42 251 744 747 3 835.60 4.22 252 744 748 4 820.55 1.86 253 744 808 64 435.59 1.66 254 744 811 67 435.59 1.66 255 744 812 68 435.54 1.69 256 745 748 3 839.88 6.05 257 745 749 4 812.97 1.92 258 746 749 3 833.74 1.84 259 746 750 4 820.62 2.46 260 746 787 41 519.60 3.02 261 746 790 44 519.60 3.02 262 746 866 120 424.84 3.23 263 746 869 123 424.17 3.33 264 746 873 127 413.71 1.69 265 747 750 3 847.24 5.07 266 747 751 4 820.67 2.47 267 747 790 43 519.60 3.02 268 747 794 47 519.56 3.00 269 747 804 57 437.85 1.07 270 747 807 60 437.18 1.40 271 747 808 61 437.17 1.40 272 747 811 64 437.13 1.40 273 747 862 115 427.05 3.56 274 747 863 116 426.74 3.40 275 747 866 119 424.57 3.42 276 748 751 3 838.30 3.49 277 748 752 4 820.64 2.46 278 748 804 56 437.26 1.41 279 748 808 60 437.15 1.41 280 749 752 3 831.28 5.77 281 749 753 4 820.64 2.46 282 749 762 13 522.78 3.20 283 749 763 14 522.78 3.20 284 749 764 15 522.70 3.17 285 749 787 38 519.76 3.06 286 750 753 3 842.99 0.51 287 750 754 4 818.88 1.57 288 750 790 40 519.69 3.04 289 750 791 41 519.62 3.02 290 750 794 44 519.63 3.02 291 751 754 3 836.19 5.25 292 751 755 4 807.66 2.28 293 751 794 43 519.57 2.99 294 751 799 48 439.39 1.56 295 751 800 49 439.42 1.57 296 751 801 50 439.39 1.58 297 751 804 53 437.84 1.70 298 752 755 3 841.84 0.07 299 752 756 4 793.76 1.20 300 752 763 11 522.97 3.11 301 752 764 12 523.00 3.10 302 753 757 4 785.19 5.48 303 753 758 5 712.22 26.85 304 753 759 6 527.05 3.88 305 753 761 8 524.19 2.50 306 753 762 9 522.99 3.10 307 753 763 10 522.96 3.10 308 753 791 38 519.75 3.03 309 754 758 4 563.17 14.11 310 754 759 5 525.61 3.15 311 754 791 37 519.74 3.00 312 754 794 40 519.68 3.03 313 754 795 41 519.66 3.03 314 756 763 7 524.28 2.55 315 757 760 3 825.51 2.28 316 757 761 4 771.73 6.58 317 757 763 6 524.35 2.54 318 759 788 29 519.94 3.00 319 759 791 32 519.94 3.00 320 759 792 33 519.88 2.98 321 761 769 8 523.35 3.25 322 761 770 9 523.29 3.27 323 761 771 10 523.26 3.29 324 762 769 7 523.40 3.27 325 762 770 8 523.29 3.27 326 762 775 13 523.27 3.29 327 762 784 22 520.04 2.98 328 762 787 25 519.94 3.00 329 762 788 26 519.91 2.98 330 763 766 3 889.21 3.02 331 763 769 6 523.38 3.27 332 764 768 4 790.15 0.56 333 764 770 6 553.33 6.67 334 764 784 20 520.09 3.00 335 764 787 23 520.00 3.02 336 765 768 3 830.48 6.23 337 765 769 4 822.96 2.47 338 766 769 3 868.33 3.56 339 768 774 6 814.73 6.57 340 769 774 5 821.59 10.00 341 770 774 4 814.73 6.57 342 770 775 5 814.68 6.55 343 770 778 8 779.50 2.73 344 770 784 14 520.16 3.01 345 771 774 3 842.96 0.68 346 771 775 4 827.67 6.96 347 772 775 3 837.79 1.92 348 772 776 4 830.48 5.56 349 772 781 9 646.73 24.91 350 773 776 3 849.10 1.06 351 773 777 4 820.99 3.43 352 774 777 3 834.60 3.51 353 774 778 4 827.67 6.95 354 775 778 3 846.05 1.50 355 775 779 4 830.96 6.42 356 775 780 5 778.78 6.49 357 775 781 6 607.38 5.23 358 775 784 9 522.45 4.12 359 776 779 3 849.85 0.53 360 776 781 5 685.01 5.79 361 778 876 98 422.39 3.66 362 779 876 97 422.37 3.67 363 779 887 108 316.02 0.17 364 780 783 3 847.55 3.00 365 780 784 4 829.07 6.24 366 780 873 93 423.92 3.20 367 780 876 96 422.25 3.73 368 781 784 3 837.62 1.96 369 781 785 4 829.07 6.24 370 782 785 3 842.88 0.67 371 782 786 4 842.88 0.68 372 783 786 3 844.41 0.07 373 783 787 4 844.44 0.10 374 783 872 89 423.94 3.20 375 783 873 90 423.92 3.20 376 783 876 93 422.06 3.64 377 784 787 3 844.50 0.07 378 784 788 4 844.45 0.10 379 785 788 3 844.46 0.10 380 785 789 4 844.46 0.10 381 786 789 3 844.46 0.10 382 786 790 4 844.46 0.10 383 786 868 82 425.65 3.04 384 786 869 83 425.13 3.09 385 786 872 86 424.40 3.27 386 787 790 3 835.98 4.34 387 787 791 4 835.97 4.34 388 787 869 82 424.95 3.10 389 787 873 86 424.01 3.15 390 788 791 3 837.33 3.67 391 788 792 4 833.33 5.67 392 789 792 3 852.21 3.76 393 789 793 4 833.33 5.67 394 789 796 7 808.84 3.73 395 790 793 3 833.77 5.45 396 790 794 4 835.47 6.72 397 790 862 72 427.22 3.62 398 790 865 75 426.97 3.52 399 790 866 76 426.94 3.51 400 790 869 79 425.59 3.05 401 791 794 3 843.60 8.29 402 792 795 3 841.70 7.33 403 792 796 4 817.26 0.24 404 793 796 3 841.70 7.33 405 793 797 4 817.00 0.36 406 793 862 69 427.95 3.73 407 794 798 4 790.52 12.88 408 794 799 5 684.11 9.15 409 794 804 10 529.68 0.12 410 794 862 68 427.93 3.72 411 795 798 3 802.22 10.06 412 797 855 58 430.13 4.62 413 797 858 61 428.53 3.87 414 797 862 65 428.50 3.89 415 798 855 57 430.22 4.66 416 799 803 4 824.62 1.08 417 799 804 5 816.28 0.09 418 799 807 8 781.87 2.35 419 799 855 56 430.23 4.67 420 799 859 60 430.10 4.71 421 799 862 63 430.05 4.70 422 800 803 3 835.66 6.59 423 800 804 4 823.05 1.86 424 801 804 3 834.82 6.17 425 801 805 4 830.21 5.45 426 802 805 3 842.10 0.49 427 802 806 4 834.80 3.15 428 802 841 39 440.49 0.35 429 803 806 3 842.37 0.36 430 803 807 4 834.80 3.15 431 803 841 38 440.44 0.38 432 803 855 52 430.94 5.04 433 803 859 56 430.79 5.11 434 804 807 3 851.54 4.22 435 804 808 4 834.80 3.15 436 805 808 3 847.20 6.40 437 805 809 4 827.69 0.40 438 806 809 3 853.85 3.06 439 806 810 4 827.74 0.42 440 806 834 28 441.22 0.55 441 806 837 31 441.09 0.52 442 806 838 32 441.04 0.50 443 806 841 35 441.00 0.53 444 807 810 3 853.85 3.06 445 807 811 4 827.78 0.39 446 807 838 31 441.04 0.50 447 807 859 52 430.55 5.25 448 807 863 56 430.31 5.21 449 808 811 3 845.03 2.50 450 808 812 4 835.81 3.62 451 809 812 3 848.78 0.57 452 809 813 4 837.93 4.68 453 809 834 25 441.39 0.49 454 810 813 3 847.10 4.42 455 810 814 4 841.61 7.16 456 810 831 21 441.42 0.48 457 810 834 24 441.39 0.49 458 810 835 25 441.39 0.49 459 810 838 28 441.34 0.50 460 810 863 53 430.42 5.28 461 810 916 106 295.58 1.01 462 811 814 3 842.24 6.84 463 811 863 52 429.89 5.54 464 811 866 55 429.30 5.79 465 811 867 56 429.23 5.79 466 813 827 14 441.68 0.56 467 813 830 17 441.63 0.57 468 813 831 18 441.60 0.57 469 813 834 21 441.56 0.55 470 813 914 101 296.49 1.22 471 814 831 17 441.62 0.56 472 814 867 53 429.18 5.83 473 814 913 99 296.57 1.25 474 814 914 100 296.49 1.22 475 815 906 91 299.87 2.24 476 815 911 96 298.37 1.88 477 815 912 97 297.47 1.51 478 815 913 98 296.50 1.21 479 815 914 99 296.50 1.21 480 816 821 5 679.27 40.02 481 816 824 8 442.63 0.56 482 816 827 11 442.01 0.57 483 816 911 95 298.62 1.77 484 816 912 96 297.81 1.62 485 816 914 98 297.43 1.53 486 817 820 3 828.61 4.92 487 817 821 4 779.52 10.11 488 817 910 93 298.81 1.78 489 817 911 94 298.39 1.88 490 818 909 91 299.86 2.23 491 818 910 92 298.99 1.85 492 818 912 94 298.68 1.79 493 820 824 4 777.86 1.67 494 821 824 3 834.56 0.83 495 821 827 6 783.51 13.06 496 823 826 3 854.25 1.38 497 823 827 4 827.84 2.55 498 824 827 3 838.62 1.83 499 824 828 4 830.90 1.02 500 825 828 3 837.15 2.58 501 825 829 4 835.61 3.35 502 826 829 3 838.65 1.83 503 826 830 4 840.39 3.71 504 827 830 3 845.46 1.18 505 827 831 4 845.05 1.38 506 827 914 87 297.78 1.59 507 828 831 3 840.40 3.71 508 828 832 4 839.91 3.95 509 829 832 3 839.97 3.98 510 829 833 4 832.61 7.66 511 830 833 3 845.52 1.21 512 830 834 4 832.61 7.65 513 831 834 3 840.80 3.71 514 831 835 4 833.05 7.42 515 831 914 83 296.51 1.23 516 831 916 85 296.48 1.22 517 832 835 3 842.90 2.66 518 832 836 4 833.04 7.42 519 833 836 3 842.89 2.65 520 833 837 4 833.22 7.50 521 834 837 3 830.74 3.43 522 834 838 4 833.21 2.19 523 835 838 3 836.08 0.75 524 835 839 4 845.68 4.05 525 835 856 21 431.07 5.21 526 835 916 81 295.76 0.89 527 836 839 3 842.92 5.43 528 836 840 4 842.91 5.43 529 837 840 3 842.92 5.42 530 837 841 4 843.66 5.05 531 838 841 3 843.67 5.05 532 838 842 4 825.13 1.95 533 838 856 18 431.16 5.25 534 838 859 21 430.81 5.12 535 839 842 3 830.60 0.78 536 839 843 4 825.15 1.94 537 839 856 17 431.17 5.26 538 840 843 3 850.83 0.91 539 840 844 4 830.59 0.78 540 841 844 3 844.93 2.04 541 842 846 4 840.09 3.98 542 842 852 10 431.30 5.25 543 842 855 13 431.26 5.25 544 842 856 14 431.26 5.25 545 842 859 17 431.22 5.26 546 843 846 3 840.09 3.98 547 843 852 9 431.31 5.24 548 846 851 5 703.19 32.16 549 846 852 6 608.14 24.20 550 846 855 9 537.12 27.32 551 848 851 3 822.99 3.42 552 848 852 4 823.09 5.94 553 849 852 3 826.82 7.80 554 849 853 4 826.82 7.79 555 850 853 3 838.90 1.76 556 850 854 4 830.24 2.36 557 851 854 3 856.68 2.00 558 851 855 4 827.52 1.01 559 852 855 3 837.37 3.92 560 852 856 4 831.83 1.15 561 853 856 3 840.86 4.59 562 853 857 4 826.24 3.08 563 854 857 3 833.15 0.74 564 854 858 4 827.06 2.70 565 855 858 3 847.68 6.52 566 855 859 4 827.06 2.70 567 856 859 3 855.33 2.69 568 856 860 4 831.29 1.67 569 856 916 60 294.62 0.82 570 857 860 3 870.40 4.46 571 857 861 4 836.03 1.81 572 857 917 60 293.58 1.16 573 858 861 3 840.39 0.36 574 858 862 4 836.02 1.81 575 859 862 3 836.02 1.82 576 859 863 4 830.00 4.83 577 860 863 3 830.78 5.21 578 860 864 4 834.68 2.48 579 860 916 56 293.57 1.16 580 860 917 57 293.56 1.17 581 861 864 3 849.19 3.99 582 861 865 4 837.69 1.77 583 861 917 56 293.54 1.17 584 862 865 3 839.04 1.09 585 862 866 4 839.14 1.04 586 863 866 3 855.56 0.76 587 863 916 53 293.57 1.16 588 864 867 3 867.30 5.12 589 864 868 4 857.61 0.27 590 864 915 51 293.56 1.16 591 864 916 52 293.55 1.17 592 864 917 53 293.50 1.17 593 865 868 3 869.31 4.12 594 865 869 4 849.27 2.21 595 866 869 3 857.62 0.27 596 866 870 4 833.61 1.68 597 867 870 3 846.37 2.75 598 867 871 4 838.85 1.01 599 867 913 46 295.32 1.53 600 867 915 48 294.34 1.35 601 868 871 3 842.35 2.78 602 868 872 4 838.83 1.02 603 868 915 47 294.98 1.67 604 869 872 3 838.84 1.03 605 869 873 4 832.54 2.12 606 870 873 3 838.82 1.03 607 870 874 4 830.35 2.17 608 871 874 3 833.79 0.44 609 871 875 4 824.73 4.97 610 871 906 35 300.49 1.94 611 871 907 36 300.44 1.92 612 872 875 3 861.51 3.32 613 872 876 4 819.74 7.29 614 873 876 3 845.50 5.42 615 873 877 4 819.91 7.38 616 874 877 3 845.40 4.63 617 874 878 4 817.78 1.32 618 874 903 29 300.53 1.95 619 874 906 32 300.53 1.95 620 874 907 33 300.51 1.96 621 875 878 3 845.41 4.62 622 875 879 4 825.12 5.00 623 875 907 32 300.51 1.96 624 876 879 3 834.84 0.14 625 876 880 4 819.81 2.35 626 877 880 3 842.14 12.58 627 877 881 4 825.68 5.39 628 877 891 14 302.74 1.99 629 877 899 22 301.20 2.02 630 878 881 3 835.89 0.30 631 878 882 4 835.88 0.30 632 878 899 21 301.22 2.04 633 878 903 25 300.87 1.96 634 878 904 26 300.75 1.90 635 878 907 29 300.64 1.95 636 879 882 3 835.89 0.29 637 879 883 4 835.88 0.30 638 880 883 3 835.88 0.30 639 880 884 4 817.93 6.58 640 880 887 7 654.44 28.20 641 880 889 9 375.18 37.38 642 880 891 11 304.35 2.79 643 881 884 3 833.25 4.33 644 881 891 10 304.33 2.78 645 881 896 15 301.72 2.12 646 881 899 18 301.47 2.02 647 881 900 19 301.48 2.02 648 882 885 3 990.25 10.00 649 883 886 3 837.33 0.60 650 883 887 4 835.44 0.95 651 884 887 3 840.82 1.14 652 884 888 4 823.50 5.32 653 884 889 5 377.70 36.47 654 884 891 7 375.17 37.38 655 891 895 4 819.33 8.32 656 891 896 5 768.12 10.36 657 891 899 8 708.09 19.66 658 892 895 3 836.00 0.01 659 892 896 4 820.93 7.54 660 893 896 3 850.97 6.70 661 893 897 4 828.48 3.79 662 894 897 3 835.30 1.89 663 894 898 4 830.62 2.74 664 895 898 3 833.82 1.15 665 895 899 4 830.62 2.74 666 896 899 3 833.83 1.16 667 896 900 4 828.50 3.81 668 897 900 3 843.65 6.03 669 897 901 4 819.19 8.46 670 898 901 3 833.84 2.06 671 898 902 4 791.70 21.96 672 898 903 5 791.69 21.96 673 899 903 4 779.46 16.45 674 899 904 5 793.91 9.21 675 900 904 4 834.06 1.84 676 901 904 3 862.99 2.00 677 901 905 4 841.85 2.03 678 902 905 3 853.39 3.74 679 902 906 4 841.84 2.03 680 903 906 3 854.01 3.43 681 903 907 4 841.85 2.02 682 904 907 3 841.85 2.02 683 904 908 4 834.32 1.75 684 905 908 3 842.40 1.74 685 905 909 4 834.35 1.73 686 905 910 5 668.73 37.41 687 905 911 6 455.48 1.42 688 906 909 3 834.45 1.79 689 906 911 5 521.37 27.90 690 909 912 3 855.23 5.13