BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 5 24 19 47.86 4.97 2 5 27 22 47.59 5.07 3 5 28 23 45.56 6.04 4 6 9 3 858.26 0.82 5 6 36 30 45.58 6.52 6 7 10 3 845.23 4.50 7 7 11 4 837.80 0.80 8 7 36 29 48.31 5.25 9 8 11 3 851.84 0.89 10 8 24 16 51.13 4.96 11 9 12 3 835.26 1.27 12 9 13 4 826.15 3.21 13 9 21 12 57.26 1.89 14 9 24 15 50.70 4.74 15 9 25 16 50.70 4.74 16 10 13 3 823.08 4.65 17 10 14 4 823.07 4.65 18 10 39 29 50.84 4.63 19 10 40 30 50.66 4.72 20 11 15 4 823.06 4.65 21 12 15 3 835.30 1.26 22 12 16 4 814.25 11.31 23 12 17 5 149.75 21.52 24 12 20 8 61.38 1.49 25 12 21 9 57.83 1.86 26 13 16 3 847.33 5.22 27 13 21 8 63.15 0.56 28 13 40 27 62.12 0.97 29 13 44 31 56.37 1.89 30 17 20 3 842.41 1.69 31 17 21 4 830.25 4.40 32 18 21 3 838.58 0.23 33 18 22 4 839.91 0.44 34 18 51 33 131.53 0.96 35 19 22 3 853.44 0.57 36 19 23 4 839.81 0.49 37 20 23 3 853.43 0.57 38 20 24 4 835.66 1.58 39 21 24 3 853.51 0.53 40 21 25 4 833.15 2.81 41 21 47 26 146.44 0.03 42 21 51 30 144.55 0.97 43 22 25 3 837.46 0.66 44 22 26 4 833.15 2.81 45 22 51 29 144.57 0.98 46 23 26 3 841.49 0.67 47 24 27 3 865.50 2.39 48 24 28 4 823.66 0.27 49 25 28 3 847.09 4.22 50 25 48 23 150.88 0.40 51 25 51 26 150.85 0.40 52 25 52 27 150.80 0.39 53 25 53 28 150.79 0.40 54 25 56 31 150.84 0.42 55 26 56 30 153.07 1.50 56 27 56 29 154.18 1.18 57 28 32 4 837.79 0.69 58 28 33 5 831.51 3.81 59 28 52 24 154.27 1.22 60 28 56 28 154.22 1.20 61 29 32 3 837.80 0.69 62 29 33 4 833.33 2.93 63 30 33 3 848.14 5.65 64 30 34 4 837.44 4.98 65 30 56 26 154.28 1.21 66 30 59 29 154.28 1.21 67 30 60 30 154.28 1.21 68 31 34 3 846.69 5.13 69 31 35 4 837.44 4.98 70 32 35 3 858.29 0.66 71 32 36 4 846.90 5.03 72 33 36 3 869.58 3.85 73 33 37 4 833.48 2.83 74 33 52 19 155.03 0.95 75 33 60 27 154.33 1.22 76 34 37 3 842.39 7.29 77 34 60 26 154.33 1.22 78 34 63 29 154.33 1.22 79 34 64 30 154.33 1.22 80 36 39 3 860.34 1.83 81 36 40 4 839.96 0.68 82 37 40 3 866.37 1.19 83 37 45 8 158.88 2.68 84 37 48 11 159.06 2.64 85 37 49 12 158.88 2.68 86 37 60 23 155.12 0.94 87 37 64 27 155.00 1.01 88 37 68 31 155.30 0.90 89 37 72 35 155.56 1.02 90 40 44 4 832.62 0.18 91 40 45 5 826.63 2.15 92 41 44 3 843.22 2.85 93 41 45 4 835.46 0.81 94 42 45 3 840.79 0.39 95 42 46 4 837.36 0.09 96 43 46 3 840.45 1.46 97 43 47 4 837.36 0.09 98 44 47 3 836.60 0.86 99 44 48 4 835.56 0.82 100 45 48 3 847.47 6.30 101 45 49 4 834.04 1.57 102 45 68 23 365.72 3.16 103 45 72 27 365.69 3.14 104 46 49 3 849.22 5.42 105 46 50 4 834.70 2.48 106 46 72 26 365.69 3.14 107 46 75 29 365.70 3.14 108 46 76 30 365.68 3.12 109 46 77 31 365.72 3.11 110 47 50 3 839.01 0.34 111 47 51 4 834.67 2.50 112 48 51 3 848.13 0.38 113 48 52 4 827.78 5.94 114 49 52 3 866.28 3.85 115 49 57 8 534.80 81.33 116 49 60 11 534.80 81.33 117 49 61 12 366.29 2.92 118 49 72 23 366.19 2.93 119 49 76 27 366.14 2.92 120 49 80 31 366.12 2.91 121 50 76 26 366.14 2.92 122 50 79 29 366.13 2.92 123 50 80 30 366.11 2.91 124 52 56 4 842.97 1.60 125 52 57 5 816.00 2.07 126 52 60 8 790.77 10.73 127 52 80 28 366.12 2.92 128 53 56 3 855.24 1.80 129 53 57 4 843.00 1.62 130 54 57 3 843.10 1.67 131 54 58 4 843.05 1.64 132 54 80 26 366.12 2.92 133 54 83 29 360.29 0.35 134 54 84 30 357.86 0.84 135 54 87 33 355.92 0.38 136 55 58 3 846.43 3.27 137 55 59 4 843.05 1.64 138 55 87 32 355.70 0.28 139 56 59 3 852.27 2.98 140 56 60 4 843.03 1.65 141 57 60 3 848.45 0.74 142 57 61 4 842.97 1.68 143 57 80 23 366.14 2.91 144 57 84 27 357.95 0.80 145 58 61 3 856.01 1.54 146 58 62 4 841.24 2.52 147 58 84 26 357.95 0.80 148 58 87 29 353.80 0.24 149 58 88 30 345.22 0.90 150 59 62 3 855.03 4.04 151 59 63 4 841.59 2.69 152 60 63 3 855.05 4.03 153 60 64 4 841.59 2.69 154 61 64 3 855.03 4.04 155 61 69 8 532.47 79.92 156 61 72 11 366.62 3.00 157 61 73 12 366.62 3.00 158 61 84 23 358.32 0.64 159 62 69 7 686.82 25.25 160 62 92 30 269.16 0.17 161 64 68 4 828.94 1.50 162 64 69 5 828.69 1.39 163 65 68 3 840.16 3.33 164 65 69 4 828.87 1.46 165 66 69 3 828.93 1.43 166 66 70 4 835.84 2.00 167 66 99 33 268.98 0.18 168 67 70 3 838.31 0.77 169 67 71 4 835.86 2.00 170 67 103 36 268.61 0.10 171 68 71 3 847.93 4.03 172 68 72 4 835.86 2.00 173 69 72 3 857.57 0.79 174 69 73 4 835.87 2.00 175 69 96 27 269.07 0.14 176 69 99 30 268.98 0.18 177 70 73 3 841.61 4.26 178 70 74 4 829.32 1.25 179 70 99 29 268.97 0.18 180 70 100 30 268.94 0.16 181 70 103 33 267.71 0.48 182 71 74 3 845.12 0.83 183 71 75 4 829.01 1.10 184 72 75 3 830.28 0.46 185 72 76 4 822.96 3.72 186 72 81 9 553.16 80.84 187 73 81 8 534.51 81.28 188 73 85 12 360.72 0.18 189 73 96 23 269.08 0.14 190 73 97 24 269.06 0.13 191 73 100 27 268.97 0.18 192 73 107 34 263.06 2.19 193 73 111 38 260.30 1.66 194 74 100 26 268.94 0.17 195 74 103 29 267.70 0.49 196 74 104 30 266.91 0.80 197 74 107 33 263.04 2.18 198 76 80 4 831.33 1.15 199 76 81 5 834.76 0.58 200 76 84 8 805.88 2.46 201 77 80 3 839.31 0.17 202 77 81 4 834.78 0.58 203 78 81 3 840.65 2.35 204 78 82 4 834.85 0.61 205 78 114 36 258.19 1.31 206 79 82 3 844.70 0.35 207 79 83 4 834.87 0.61 208 80 83 3 849.06 1.83 209 80 84 4 835.13 0.67 210 81 84 3 844.45 0.47 211 81 85 4 835.14 0.68 212 81 107 26 263.06 2.17 213 81 111 30 260.31 1.66 214 81 114 33 258.21 1.31 215 82 85 3 852.31 3.46 216 82 86 4 839.03 2.62 217 82 114 32 258.22 1.30 218 82 116 34 258.12 1.28 219 83 86 3 853.50 2.87 220 83 87 4 839.05 2.62 221 84 87 3 866.39 3.58 222 84 88 4 835.10 0.67 223 85 88 3 849.77 0.97 224 85 93 8 491.16 107.10 225 85 111 26 261.56 2.06 226 85 115 30 258.12 1.27 227 86 115 29 258.12 1.27 228 86 116 30 258.12 1.27 229 86 119 33 237.97 0.90 230 88 92 4 807.08 3.17 231 88 93 5 805.11 4.09 232 88 96 8 758.65 1.66 233 89 92 3 834.82 4.70 234 89 93 4 823.25 2.03 235 89 141 52 85.18 1.46 236 90 93 3 839.84 4.20 237 90 94 4 823.26 2.03 238 90 119 29 241.97 1.09 239 90 122 32 236.50 1.47 240 90 123 33 234.55 0.56 241 90 126 36 228.36 1.76 242 90 139 49 85.03 1.41 243 90 140 50 85.48 1.60 244 90 141 51 85.19 1.46 245 91 94 3 853.02 3.00 246 91 95 4 823.32 2.00 247 91 126 35 225.29 0.26 248 91 137 46 82.34 2.07 249 91 138 47 84.29 1.09 250 91 139 48 85.09 1.43 251 91 247 156 67.66 0.69 252 92 95 3 831.77 2.21 253 92 96 4 823.48 2.08 254 93 96 3 831.93 2.14 255 93 97 4 827.05 0.29 256 93 115 22 259.12 0.81 257 93 119 26 241.97 1.09 258 93 123 30 234.50 0.58 259 94 97 3 839.82 3.56 260 94 98 4 826.77 0.43 261 94 123 29 234.36 0.62 262 94 126 32 225.13 0.33 263 94 127 33 214.21 1.52 264 95 98 3 835.84 0.83 265 95 99 4 827.60 0.83 266 96 99 3 840.82 3.31 267 96 100 4 840.81 3.32 268 97 100 3 845.16 5.49 269 97 101 4 827.68 0.87 270 97 108 11 262.93 2.19 271 97 111 14 261.80 2.08 272 97 112 15 261.80 2.08 273 97 115 18 259.69 1.07 274 97 123 26 234.39 0.65 275 98 101 3 836.29 3.40 276 98 102 4 836.28 3.40 277 98 127 29 214.28 1.53 278 98 131 33 171.33 5.84 279 99 102 3 856.31 0.09 280 99 103 4 843.78 1.60 281 100 103 3 849.33 0.70 282 100 104 4 829.38 0.04 283 100 107 7 739.22 1.89 284 100 108 8 263.00 2.16 285 100 111 11 261.83 2.06 286 101 104 3 844.63 1.18 287 101 105 4 793.94 4.47 288 101 108 7 263.19 2.12 289 101 127 26 220.38 1.52 290 101 131 30 175.58 4.56 291 104 107 3 865.21 6.76 292 104 108 4 836.33 3.17 293 105 108 3 836.33 3.17 294 105 109 4 836.33 3.17 295 106 109 3 847.08 2.20 296 106 110 4 847.07 2.19 297 107 110 3 832.02 9.71 298 107 111 4 826.69 1.39 299 108 111 3 850.60 0.43 300 108 112 4 826.69 1.39 301 109 112 3 853.26 1.77 302 109 113 4 819.47 3.21 303 110 113 3 841.34 7.73 304 110 114 4 827.72 0.92 305 111 114 3 862.62 8.13 306 111 115 4 829.15 1.64 307 112 115 3 839.05 4.74 308 112 120 8 380.30 70.27 309 112 124 12 234.56 0.59 310 113 120 7 742.82 5.57 311 115 119 4 797.49 3.39 312 115 120 5 762.50 15.27 313 115 123 8 769.72 2.91 314 116 119 3 815.47 0.91 315 116 120 4 805.26 0.64 316 117 120 3 816.39 1.38 317 117 121 4 817.32 5.40 318 118 122 4 827.18 0.51 319 119 122 3 860.53 1.95 320 119 123 4 837.78 0.41 321 120 123 3 847.62 4.51 322 120 124 4 833.40 1.79 323 121 124 3 852.02 3.56 324 121 125 4 841.28 5.73 325 122 125 3 861.64 1.26 326 122 126 4 833.39 1.79 327 122 140 18 145.34 15.29 328 123 126 3 827.93 0.93 329 123 127 4 826.09 1.81 330 124 128 4 833.62 1.96 331 125 128 3 861.47 1.49 332 125 129 4 860.34 1.91 333 126 129 3 867.50 1.67 334 126 138 12 108.92 2.99 335 126 140 14 148.25 15.82 336 129 135 6 425.04 2.79 337 129 136 7 426.04 3.31 338 129 138 9 425.98 3.29 339 129 211 82 434.91 1.11 340 130 133 3 807.42 4.15 341 130 136 6 453.72 0.85 342 131 136 5 456.39 2.19 343 132 214 82 500.60 1.25 344 132 215 83 500.50 1.28 345 133 136 3 833.99 2.71 346 133 137 4 818.25 6.28 347 133 211 78 500.86 1.26 348 133 212 79 500.84 1.25 349 133 213 80 500.78 1.28 350 133 214 81 500.61 1.26 351 133 215 82 500.58 1.26 352 134 137 3 842.88 1.73 353 134 138 4 818.25 6.29 354 134 209 75 500.67 1.28 355 134 210 76 500.86 1.26 356 134 211 77 500.88 1.25 357 134 213 79 500.83 1.25 358 134 218 84 500.61 1.25 359 134 232 98 500.70 1.19 360 134 243 109 497.00 0.41 361 135 138 3 840.09 2.73 362 135 211 76 500.98 1.27 363 137 215 78 501.57 1.06 364 137 218 81 501.54 1.05 365 137 243 106 500.88 1.26 366 137 251 114 89.20 3.23 367 138 211 73 502.23 0.98 368 138 232 94 501.91 1.10 369 138 240 102 502.46 0.92 370 138 241 103 501.91 1.10 371 138 242 104 501.09 1.28 372 138 243 105 500.94 1.26 373 139 142 3 921.09 0.41 374 139 166 27 615.12 57.65 375 139 241 102 502.36 0.95 376 139 242 103 502.00 1.10 377 140 240 100 502.55 0.90 378 140 241 101 502.37 0.96 379 141 164 23 618.53 56.04 380 141 165 24 617.88 56.31 381 141 166 25 617.85 56.33 382 142 239 97 511.27 2.41 383 142 240 98 511.15 2.37 384 142 241 99 510.33 2.54 385 142 242 100 505.71 2.17 386 143 157 14 619.47 55.65 387 143 158 15 618.70 56.00 388 143 161 18 618.73 56.01 389 143 165 22 618.62 56.05 390 143 167 24 618.60 56.06 391 143 168 25 618.43 56.05 392 143 170 27 618.35 56.02 393 143 171 28 618.00 55.86 394 143 172 29 617.91 55.72 395 144 157 13 619.59 55.63 396 144 171 27 618.39 55.95 397 144 172 28 617.97 55.74 398 144 174 30 618.51 55.41 399 144 239 95 511.49 2.35 400 145 149 4 825.11 2.98 401 145 154 9 619.59 55.63 402 145 157 12 619.60 55.63 403 145 171 26 618.95 55.72 404 145 172 27 618.90 55.70 405 145 173 28 618.75 55.30 406 145 174 29 618.84 55.25 407 145 198 53 548.52 19.62 408 146 149 3 855.70 1.36 409 146 173 27 619.22 55.44 410 146 174 28 618.87 55.26 411 146 175 29 618.88 55.23 412 146 176 30 618.88 55.23 413 146 198 52 548.58 19.64 414 147 173 26 619.40 55.48 415 147 181 34 608.89 50.23 416 147 198 51 548.52 19.62 417 147 199 52 548.46 19.61 418 147 202 55 547.61 19.18 419 148 197 49 552.19 17.86 420 149 153 4 824.32 7.85 421 149 154 5 804.16 2.23 422 149 197 48 552.32 17.80 423 149 198 49 552.18 17.86 424 150 153 3 840.74 0.32 425 150 154 4 831.54 9.47 426 150 194 44 597.49 39.86 427 150 195 45 588.34 35.36 428 150 196 46 552.99 17.71 429 150 197 47 552.33 17.80 430 151 154 3 838.81 5.83 431 151 155 4 810.63 0.98 432 151 189 38 612.79 47.62 433 151 192 41 611.79 47.02 434 151 193 42 606.86 44.55 435 151 194 43 588.47 35.34 436 151 196 45 553.01 17.70 437 152 155 3 851.07 0.29 438 152 156 4 805.87 1.40 439 152 194 42 588.48 35.34 440 153 156 3 837.31 5.17 441 153 157 4 807.76 2.33 442 154 157 3 838.66 5.84 443 154 158 4 810.58 0.93 444 154 189 35 612.88 47.57 445 154 198 44 552.78 17.55 446 155 158 3 845.33 2.65 447 155 159 4 828.87 2.21 448 155 186 31 612.99 47.62 449 155 189 34 612.87 47.56 450 156 159 3 861.55 0.58 451 156 160 4 843.68 4.63 452 157 160 3 843.69 4.63 453 157 161 4 843.69 4.63 454 158 161 3 837.73 1.65 455 158 162 4 837.73 1.67 456 158 168 10 624.37 53.27 457 158 171 13 624.04 53.38 458 158 186 28 613.08 47.58 459 158 189 31 613.07 47.48 460 159 162 3 862.25 2.29 461 159 163 4 862.25 2.29 462 160 163 3 911.01 2.83 463 161 164 3 871.62 0.13 464 161 165 4 834.60 0.07 465 162 165 3 849.09 3.98 466 162 168 6 624.57 53.34 467 165 168 3 879.47 1.70 468 166 242 76 516.77 0.16 469 166 244 78 516.66 0.18 470 167 229 62 516.75 0.15 471 167 242 75 516.77 0.15 472 168 171 3 864.26 0.75 473 168 186 18 676.18 16.03 474 169 185 16 676.25 16.02 475 169 186 17 676.18 16.03 476 170 183 13 676.52 15.98 477 170 184 14 676.35 15.97 478 170 185 15 676.28 16.00 479 170 228 58 517.45 0.51 480 170 229 59 516.75 0.15 481 171 183 12 676.50 15.96 482 171 184 13 676.35 15.97 483 171 185 14 676.34 15.97 484 171 186 15 676.33 15.97 485 171 189 18 676.33 15.97 486 172 180 8 677.20 16.02 487 172 182 10 676.75 15.96 488 172 183 11 676.49 15.96 489 172 206 34 590.72 25.98 490 172 229 57 549.73 16.57 491 172 230 58 549.74 16.57 492 172 231 59 549.75 16.57 493 172 239 67 574.72 20.43 494 172 242 70 549.73 16.57 495 173 180 7 677.20 16.02 496 173 181 8 677.15 16.01 497 173 182 9 676.75 15.96 498 173 198 25 615.88 14.06 499 173 202 29 610.87 16.50 500 174 178 4 677.73 15.88 501 174 179 5 677.42 16.03 502 174 180 6 677.22 16.02 503 174 181 7 677.18 16.02 504 174 239 65 591.52 26.05 505 175 178 3 803.62 15.26 506 175 179 4 735.89 13.20 507 175 181 6 677.31 15.97 508 177 238 61 591.72 26.04 509 178 238 60 591.67 26.03 510 178 239 61 591.64 26.02 511 179 237 58 591.71 26.00 512 179 238 59 591.59 26.05 513 179 239 60 591.64 26.02 514 180 206 26 607.27 18.22 515 180 208 28 591.63 25.96 516 180 231 51 591.46 26.04 517 180 233 53 591.58 26.05 518 180 237 57 591.74 26.02 519 180 239 59 591.51 26.05 520 181 202 21 610.95 16.54 521 181 204 23 610.68 16.55 522 181 206 25 607.27 18.21 523 182 202 20 610.87 16.50 524 182 203 21 610.79 16.52 525 182 204 22 610.51 16.57 526 182 206 24 590.90 25.88 527 183 202 19 610.86 16.49 528 183 203 20 610.75 16.52 529 183 206 23 589.45 25.35 530 184 188 4 830.85 1.09 531 184 189 5 822.11 1.78 532 184 198 14 615.82 14.05 533 184 201 17 610.89 16.48 534 184 202 18 610.76 16.50 535 185 188 3 843.43 4.16 536 185 189 4 825.80 3.61 537 186 189 3 837.86 6.95 538 186 190 4 826.30 3.87 539 187 190 3 850.62 8.29 540 187 191 4 829.00 2.52 541 188 191 3 838.36 0.41 542 188 192 4 812.56 2.99 543 188 201 13 615.38 14.22 544 189 192 3 841.60 5.69 545 192 201 9 615.82 14.07 546 193 196 3 836.67 6.13 547 196 200 4 844.00 0.27 548 196 201 5 803.84 0.67 549 197 200 3 852.59 1.09 550 197 201 4 844.00 0.27 551 198 201 3 847.60 2.06 552 198 202 4 817.11 5.96 553 199 202 3 840.36 1.55 554 203 206 3 850.56 0.35 555 206 231 25 602.36 26.04 556 207 225 18 602.73 25.84 557 207 227 20 602.64 25.88 558 207 230 23 602.77 25.82 559 207 231 24 602.80 25.82 560 208 221 13 603.09 25.67 561 208 231 23 602.76 25.84 562 208 233 25 605.70 26.28 563 209 213 4 695.62 17.72 564 209 218 9 603.77 25.33 565 209 221 12 603.12 25.67 566 209 230 21 603.43 25.53 567 209 231 22 605.23 26.43 568 209 232 23 605.23 26.43 569 209 233 24 605.80 26.28 570 210 213 3 815.53 1.00 571 210 232 22 605.78 26.28 572 210 233 23 605.90 26.27 573 210 234 24 606.00 26.29 574 210 235 25 606.12 26.29 575 210 237 27 606.14 26.31 576 211 232 21 605.96 26.30 577 211 233 22 606.00 26.29 578 211 234 23 606.07 26.29 579 211 240 29 606.02 26.27 580 213 217 4 810.55 1.85 581 213 218 5 810.52 1.84 582 214 217 3 833.30 5.77 583 214 218 4 823.20 0.73 584 215 218 3 829.13 2.23 585 215 219 4 823.19 0.74 586 215 251 36 584.71 33.85 587 216 219 3 839.79 3.10 588 216 220 4 825.98 0.67 589 217 220 3 823.23 0.72 590 217 221 4 823.18 0.73 591 218 221 3 845.30 0.36 592 218 222 4 807.75 1.08 593 218 230 12 608.91 26.79 594 218 243 25 606.11 26.38 595 218 248 30 605.63 26.49 596 219 222 3 838.87 2.44 597 219 223 4 793.75 6.77 598 219 248 29 603.51 27.55 599 219 251 32 602.65 27.51 600 220 223 3 842.93 1.60 601 220 224 4 793.75 6.76 602 221 224 3 834.66 7.56 603 221 225 4 789.74 4.73 604 221 230 9 611.73 25.77 605 222 226 4 769.31 0.31 606 222 227 5 635.73 25.68 607 222 228 6 627.98 21.80 608 222 229 7 614.29 26.94 609 222 230 8 610.89 26.21 610 222 243 21 608.98 26.75 611 222 244 22 608.94 26.75 612 222 245 23 606.38 26.31 613 222 248 26 605.85 26.51 614 223 226 3 800.26 9.65 615 227 230 3 897.12 4.24 616 227 245 18 608.95 26.75 617 228 244 16 608.95 26.75 618 228 245 17 608.90 26.75 619 229 242 13 610.35 26.69 620 229 243 14 609.69 26.44 621 229 244 15 608.93 26.75 622 230 242 12 610.39 26.71 623 230 243 13 609.78 26.45 624 231 239 8 612.79 27.09 625 231 241 10 612.19 27.08 626 231 242 11 610.72 26.55 627 232 239 7 612.80 27.09 628 232 240 8 612.75 27.07 629 232 241 9 612.22 27.07 630 232 242 10 612.19 27.08 631 232 243 11 612.19 27.08 632 233 237 4 613.70 26.84 633 233 238 5 612.84 27.09 634 233 239 6 612.81 27.09 635 233 240 7 612.79 27.09 636 234 237 3 824.40 1.56 637 234 238 4 613.70 26.84 638 234 240 6 612.81 27.09 639 243 248 5 816.72 7.62 640 244 247 3 860.29 3.46 641 244 248 4 826.94 2.52 642 245 248 3 826.94 2.52 643 245 249 4 826.94 2.52 644 246 249 3 837.72 7.91 645 246 250 4 841.16 6.19 646 247 250 3 844.39 4.57 647 247 251 4 843.00 5.27 648 248 251 3 844.39 4.57 649 248 252 4 837.00 2.27 650 249 252 3 835.12 3.21