BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 203 201 161.78 1.66 2 2 204 202 153.40 2.54 3 2 205 203 149.65 1.62 4 2 227 225 90.41 0.01 5 4 35 31 567.65 52.59 6 4 36 32 567.58 52.65 7 4 37 33 567.55 52.64 8 4 205 201 172.58 1.10 9 5 36 31 567.64 52.63 10 5 37 32 567.63 52.62 11 5 227 222 111.87 0.33 12 5 228 223 111.85 0.33 13 5 243 238 47.15 0.33 14 5 244 239 43.17 0.82 15 5 246 241 37.30 1.16 16 5 247 242 23.75 0.73 17 5 248 243 17.73 0.28 18 6 37 31 567.64 52.63 19 6 39 33 567.83 52.52 20 6 90 84 352.74 0.48 21 6 124 118 338.69 0.29 22 6 161 155 334.10 0.56 23 6 205 199 187.47 0.55 24 6 207 201 169.29 0.59 25 6 227 221 112.03 0.29 26 6 228 222 111.89 0.31 27 6 229 223 111.86 0.33 28 7 37 30 567.75 52.59 29 7 38 31 567.59 52.65 30 7 39 32 567.83 52.52 31 7 229 222 111.88 0.31 32 7 231 224 111.83 0.33 33 7 240 233 57.75 1.16 34 7 243 236 47.72 0.04 35 7 244 237 42.91 0.95 36 8 39 31 567.84 52.52 37 8 229 221 112.23 0.19 38 8 230 222 111.84 0.32 39 8 231 223 111.83 0.33 40 8 240 232 57.81 1.20 41 9 39 30 567.88 52.53 42 9 40 31 567.86 52.52 43 9 41 32 567.94 52.47 44 9 231 222 111.88 0.31 45 9 235 226 82.50 0.11 46 9 240 231 57.81 1.19 47 10 41 31 567.95 52.47 48 10 64 54 460.76 0.57 49 10 95 85 357.62 0.21 50 10 230 220 116.95 1.26 51 10 231 221 112.91 0.42 52 10 232 222 111.81 0.34 53 10 235 225 96.91 0.52 54 11 14 3 863.62 1.67 55 11 23 12 568.20 52.40 56 11 40 29 568.08 52.44 57 11 41 30 568.06 52.44 58 11 42 31 568.04 52.43 59 11 43 32 568.06 52.42 60 11 235 224 109.39 1.29 61 11 236 225 85.90 1.17 62 12 43 31 568.07 52.43 63 12 64 52 461.63 0.14 64 12 95 83 357.88 0.21 65 12 97 85 357.94 0.22 66 12 170 158 317.64 1.67 67 12 232 220 116.92 1.23 68 14 43 29 568.09 52.43 69 14 236 222 111.68 0.34 70 15 19 4 844.65 1.05 71 15 46 31 568.77 52.08 72 16 19 3 856.00 4.62 73 16 20 4 855.99 4.62 74 16 46 30 568.81 52.06 75 17 20 3 864.37 0.44 76 17 21 4 855.99 4.62 77 17 46 29 568.81 52.06 78 17 49 32 552.80 43.88 79 18 21 3 876.39 3.14 80 18 22 4 864.37 0.44 81 19 22 3 860.16 2.52 82 19 23 4 856.89 4.16 83 20 23 3 843.13 1.06 84 20 24 4 838.46 1.27 85 20 46 26 569.17 51.88 86 20 49 29 538.79 36.59 87 20 50 30 525.24 29.88 88 21 25 4 823.69 1.90 89 21 49 28 539.38 36.88 90 21 53 32 509.34 21.87 91 22 25 3 882.53 10.69 92 22 26 4 831.53 2.02 93 23 26 3 882.54 10.69 94 23 27 4 840.12 1.78 95 23 235 212 103.95 1.15 96 23 236 213 86.86 1.11 97 24 27 3 849.16 2.74 98 24 28 4 840.07 1.80 99 24 40 16 569.32 51.94 100 24 50 26 513.97 24.17 101 24 53 29 465.40 0.35 102 24 54 30 465.31 0.39 103 25 28 3 850.69 3.50 104 25 29 4 840.08 1.79 105 26 29 3 863.12 6.17 106 26 30 4 840.40 2.29 107 26 236 210 91.70 0.18 108 26 237 211 83.84 0.13 109 26 238 212 76.25 0.35 110 26 241 215 64.65 2.27 111 27 30 3 855.52 3.45 112 27 38 11 570.11 51.55 113 27 56 29 465.66 0.69 114 27 59 32 465.11 0.74 115 27 240 213 64.86 2.37 116 28 53 25 465.85 0.58 117 28 54 26 465.69 0.67 118 28 55 27 465.66 0.69 119 28 56 28 465.61 0.71 120 30 56 26 483.92 9.86 121 30 241 211 57.27 1.31 122 31 245 214 50.16 0.13 123 32 36 4 863.87 6.26 124 32 56 24 509.55 22.68 125 32 58 26 501.94 19.46 126 32 241 209 56.93 1.24 127 32 244 212 48.94 0.57 128 32 245 213 45.61 0.04 129 33 36 3 888.90 2.65 130 33 58 25 510.20 23.59 131 33 245 212 45.89 0.20 132 33 247 214 44.70 0.33 133 34 58 24 509.93 23.81 134 36 58 22 510.78 24.28 135 37 58 21 510.84 24.31 136 37 90 53 353.59 0.71 137 37 205 168 188.34 0.18 138 38 58 20 510.88 24.30 139 38 59 21 510.79 24.29 140 38 60 22 510.79 24.29 141 38 240 202 57.77 1.17 142 38 244 206 45.54 0.07 143 39 60 21 510.79 24.29 144 39 62 23 510.64 24.36 145 39 90 51 353.60 0.71 146 39 124 85 350.31 0.67 147 40 50 10 512.06 23.77 148 40 54 14 511.95 23.82 149 40 59 19 511.27 24.12 150 40 60 20 511.25 24.12 151 40 61 21 510.74 24.35 152 40 62 22 510.69 24.34 153 40 240 200 60.19 0.04 154 41 62 21 510.58 24.43 155 41 63 22 510.52 24.43 156 41 64 23 510.47 24.44 157 41 92 51 354.98 0.18 158 41 124 83 354.85 0.24 159 42 47 5 684.54 5.29 160 42 50 8 558.17 0.91 161 42 61 19 511.29 24.12 162 42 62 20 511.25 24.14 163 42 64 22 510.50 24.44 164 43 46 3 874.36 3.45 165 43 47 4 835.91 5.04 166 43 64 21 510.54 24.44 167 44 47 3 859.44 0.71 168 44 78 34 360.24 0.34 169 46 49 3 843.87 8.03 170 46 50 4 833.25 5.96 171 47 50 3 863.12 1.59 172 47 51 4 847.18 4.46 173 47 61 14 511.66 24.00 174 47 62 15 511.63 23.98 175 47 63 16 511.59 24.00 176 47 83 36 356.82 0.52 177 47 86 39 356.67 0.57 178 47 88 41 355.97 0.35 179 48 51 3 848.89 5.32 180 48 52 4 852.62 3.46 181 48 86 38 356.01 0.34 182 49 52 3 862.97 1.72 183 49 53 4 847.75 1.02 184 50 53 3 862.00 2.20 185 50 54 4 847.76 1.02 186 50 61 11 511.65 24.00 187 51 54 3 875.71 2.29 188 51 61 10 511.66 24.00 189 51 86 35 356.24 0.24 190 51 87 36 354.70 0.12 191 54 59 5 511.92 24.00 192 54 61 7 511.71 24.01 193 56 59 3 864.14 4.99 194 60 89 29 354.93 0.22 195 60 90 30 354.81 0.28 196 61 88 27 355.29 0.24 197 61 89 28 354.94 0.23 198 62 88 26 356.00 0.34 199 62 90 28 354.77 0.26 200 63 83 20 357.62 0.15 201 63 90 27 355.70 0.22 202 63 91 28 355.01 0.19 203 63 92 29 355.17 0.26 204 64 92 28 356.26 0.52 205 65 78 13 360.37 0.41 206 65 91 26 357.58 0.10 207 65 92 27 357.63 0.07 208 65 93 28 357.60 0.06 209 65 98 33 358.10 0.11 210 65 112 47 359.94 0.41 211 66 78 12 360.37 0.41 212 66 80 14 360.27 0.39 213 66 83 17 360.34 0.39 214 66 91 25 358.23 0.12 215 66 93 27 358.06 0.04 216 66 112 46 359.97 0.42 217 67 74 7 362.35 0.85 218 67 77 10 360.98 0.70 219 67 78 11 360.94 0.69 220 67 108 41 360.23 0.40 221 67 112 45 360.19 0.39 222 68 108 40 360.25 0.41 223 68 111 43 360.32 0.44 224 68 112 44 360.19 0.40 225 68 115 47 360.35 0.43 226 69 74 5 817.76 1.39 227 69 115 46 360.96 0.68 228 70 79 9 361.35 0.79 229 70 81 11 361.38 0.80 230 70 115 45 361.32 0.78 231 70 119 49 361.37 0.80 232 71 74 3 973.60 0.21 233 74 77 3 873.63 0.75 234 78 83 5 830.74 0.96 235 79 82 3 855.39 0.33 236 79 83 4 832.94 0.12 237 80 83 3 852.74 2.75 238 80 84 4 830.79 0.99 239 80 91 11 419.94 26.37 240 80 115 35 515.28 23.05 241 80 116 36 514.99 23.05 242 80 119 39 516.26 22.90 243 80 121 41 515.44 23.24 244 81 84 3 849.09 0.04 245 81 85 4 840.96 4.09 246 81 119 38 516.40 22.83 247 82 85 3 844.59 2.28 248 82 86 4 840.96 4.09 249 83 86 3 849.79 0.32 250 83 87 4 840.96 4.09 251 83 88 5 684.89 64.92 252 83 90 7 579.25 54.41 253 84 87 3 847.37 5.21 254 84 88 4 697.82 58.47 255 84 119 35 564.76 28.00 256 84 120 36 558.32 24.78 257 84 121 37 543.09 29.00 258 89 121 32 603.26 29.50 259 89 122 33 603.26 29.50 260 90 121 31 603.28 29.50 261 90 122 32 603.26 29.50 262 90 123 33 603.25 29.50 263 90 124 34 603.25 29.50 264 90 159 69 603.25 29.50 265 90 161 71 603.25 29.50 266 91 116 25 603.47 29.48 267 91 121 30 603.39 29.50 268 91 122 31 603.31 29.50 269 91 123 32 603.27 29.50 270 91 124 33 603.26 29.50 271 92 95 3 924.56 1.53 272 92 124 32 603.26 29.50 273 92 126 34 603.25 29.50 274 92 230 138 117.27 1.29 275 93 109 16 603.40 29.50 276 93 112 19 603.48 29.48 277 93 113 20 603.45 29.50 278 93 123 30 603.39 29.50 279 93 124 31 603.34 29.50 280 93 125 32 603.27 29.50 281 93 126 33 603.25 29.50 282 94 99 5 814.98 3.33 283 94 109 15 603.41 29.50 284 94 126 32 603.27 29.50 285 95 98 3 863.23 5.44 286 95 99 4 845.23 1.03 287 95 126 31 603.30 29.50 288 95 170 75 446.97 66.33 289 96 99 3 846.95 1.89 290 96 100 4 845.22 1.03 291 96 167 71 583.55 30.19 292 96 170 74 451.00 68.33 293 97 100 3 871.19 1.46 294 97 101 4 825.68 2.02 295 97 102 5 825.66 2.02 296 97 170 73 547.03 24.96 297 98 102 4 700.65 0.06 298 98 103 5 700.65 0.06 299 98 104 6 603.77 29.46 300 99 104 5 603.75 29.46 301 99 109 10 603.75 29.46 302 99 126 27 603.41 29.46 303 99 146 47 603.44 29.47 304 100 103 3 846.98 2.44 305 104 108 4 847.08 1.79 306 104 109 5 827.81 2.08 307 104 112 8 822.76 0.28 308 105 108 3 857.25 0.46 309 105 109 4 847.08 1.79 310 106 109 3 849.91 3.21 311 106 110 4 835.56 1.78 312 106 149 43 603.71 29.41 313 107 110 3 841.30 1.09 314 107 111 4 841.32 1.08 315 108 111 3 859.49 1.62 316 108 112 4 843.99 1.80 317 109 112 3 856.74 0.25 318 109 113 4 847.50 1.82 319 109 125 16 603.58 29.46 320 110 113 3 868.54 4.75 321 110 114 4 847.75 1.72 322 110 149 39 604.06 29.25 323 110 150 40 604.05 29.25 324 110 153 43 605.26 29.09 325 111 114 3 870.11 1.69 326 111 115 4 847.51 1.82 327 112 115 3 866.63 0.38 328 112 116 4 848.63 1.26 329 113 116 3 867.68 0.15 330 113 117 4 848.33 1.09 331 113 123 10 604.83 29.14 332 113 150 37 605.04 29.12 333 113 154 41 606.21 29.12 334 113 160 47 605.72 28.91 335 114 117 3 852.86 0.58 336 114 118 4 851.09 0.29 337 114 153 39 675.09 5.33 338 114 154 40 606.20 29.13 339 115 118 3 847.14 1.68 340 115 119 4 847.13 1.68 341 116 119 3 856.11 2.81 342 116 120 4 847.07 1.71 343 116 121 5 811.24 3.50 344 116 123 7 675.13 5.34 345 117 120 3 847.11 1.67 346 117 121 4 825.33 7.17 347 117 123 6 681.54 8.54 348 117 154 37 691.84 3.40 349 117 159 42 684.14 9.77 350 117 160 43 681.54 8.54 351 123 160 37 795.44 2.83 352 123 161 38 795.50 2.79 353 124 161 37 795.50 2.79 354 124 163 39 795.47 2.79 355 124 230 106 117.05 1.35 356 125 147 22 795.96 2.75 357 125 150 25 795.76 2.78 358 125 160 35 795.59 2.79 359 125 161 36 795.56 2.80 360 125 162 37 795.51 2.79 361 125 163 38 795.50 2.79 362 126 163 37 795.51 2.79 363 126 165 39 795.48 2.79 364 126 230 104 117.79 1.11 365 127 143 16 796.05 2.74 366 127 146 19 796.02 2.74 367 127 147 20 796.01 2.74 368 127 162 35 795.61 2.79 369 127 163 36 795.55 2.79 370 127 164 37 795.51 2.79 371 127 165 38 795.50 2.79 372 128 142 14 796.19 2.79 373 128 143 15 796.08 2.75 374 128 146 18 796.08 2.76 375 128 164 36 795.54 2.79 376 128 165 37 795.51 2.79 377 128 166 38 795.48 2.79 378 128 167 39 795.33 2.73 379 129 133 4 841.86 0.79 380 129 134 5 841.72 0.72 381 129 139 10 796.22 2.78 382 129 164 35 795.62 2.79 383 129 166 37 795.49 2.79 384 129 167 38 795.49 2.79 385 129 168 39 795.48 2.78 386 130 133 3 865.48 0.99 387 130 134 4 853.46 5.00 388 130 167 37 795.41 2.76 389 130 168 38 795.41 2.76 390 131 134 3 865.46 0.99 391 131 135 4 853.46 5.00 392 131 168 37 795.48 2.79 393 132 135 3 865.46 0.99 394 132 136 4 853.46 5.00 395 133 136 3 852.01 4.28 396 133 137 4 841.82 0.81 397 133 138 5 824.61 7.77 398 133 142 9 796.26 2.82 399 134 137 3 841.67 0.80 400 134 139 5 807.11 0.09 401 134 168 34 795.56 2.79 402 138 141 3 881.11 2.17 403 139 142 3 868.22 4.28 404 139 143 4 858.73 0.47 405 139 164 25 795.70 2.77 406 139 184 45 241.93 2.53 407 140 143 3 862.03 2.12 408 140 144 4 842.90 1.79 409 140 184 44 241.70 2.62 410 140 187 47 241.37 2.58 411 141 144 3 856.42 4.94 412 141 145 4 852.60 6.86 413 142 145 3 872.83 3.26 414 142 146 4 850.88 7.71 415 143 146 3 862.86 1.73 416 143 147 4 831.00 2.29 417 143 164 21 795.60 2.78 418 143 188 45 240.61 2.58 419 144 147 3 841.50 0.28 420 144 148 4 853.88 6.25 421 144 191 47 240.56 2.56 422 145 148 3 858.52 3.93 423 145 149 4 857.97 4.21 424 146 149 3 869.76 1.69 425 146 150 4 873.32 3.46 426 147 150 3 873.32 3.46 427 147 151 4 857.97 4.20 428 147 157 10 803.23 0.01 429 147 162 15 795.64 2.75 430 147 188 41 240.61 2.58 431 147 191 44 240.55 2.56 432 147 192 45 240.40 2.58 433 148 151 3 855.07 0.76 434 148 152 4 848.19 0.69 435 148 195 47 239.25 3.06 436 149 153 4 872.56 3.84 437 150 153 3 872.46 2.15 438 150 154 4 857.50 5.33 439 150 157 7 822.70 6.17 440 151 154 3 878.48 0.86 441 151 155 4 866.51 0.82 442 151 157 6 825.25 4.89 443 151 191 40 240.56 2.56 444 151 192 41 240.43 2.60 445 151 195 44 231.76 0.62 446 151 196 45 211.39 1.65 447 152 155 3 963.47 0.18 448 154 157 3 893.60 0.48 449 157 160 3 859.90 1.64 450 157 162 5 867.38 2.10 451 157 192 35 240.06 2.66 452 157 196 39 207.59 0.47 453 160 204 44 206.24 0.39 454 161 204 43 206.75 0.37 455 161 205 44 205.72 0.23 456 161 207 46 205.69 0.24 457 162 204 42 207.08 0.46 458 162 205 43 206.70 0.34 459 162 206 44 205.70 0.24 460 162 207 45 205.70 0.24 461 163 207 44 205.71 0.24 462 163 230 67 117.42 1.30 463 164 184 20 240.61 2.58 464 164 185 21 240.55 2.56 465 164 188 24 240.53 2.55 466 164 206 42 207.02 0.47 467 164 207 43 206.51 0.45 468 164 208 44 205.71 0.24 469 164 209 45 205.70 0.24 470 165 170 5 819.81 9.98 471 165 209 44 205.69 0.25 472 165 230 65 118.48 1.19 473 166 169 3 861.59 1.37 474 166 170 4 846.91 3.55 475 166 208 42 207.04 0.46 476 166 209 43 206.90 0.45 477 166 210 44 205.75 0.23 478 167 170 3 869.09 2.38 479 168 172 4 844.12 2.15 480 168 174 6 334.84 18.09 481 168 176 8 265.42 4.96 482 169 172 3 853.63 2.60 483 169 174 5 286.26 5.46 484 169 175 6 251.48 2.00 485 169 210 41 206.59 0.43 486 169 211 42 206.38 0.54 487 170 210 40 206.60 0.42 488 171 211 40 206.61 0.41 489 172 211 39 206.87 0.46 490 173 211 38 206.89 0.46 491 174 211 37 206.98 0.48 492 175 208 33 207.26 0.47 493 175 210 35 207.02 0.47 494 175 211 36 206.98 0.48 495 175 216 41 164.82 1.12 496 176 180 4 843.67 3.98 497 176 181 5 810.88 2.55 498 176 208 32 207.36 0.49 499 176 220 44 121.61 1.66 500 177 180 3 856.10 2.23 501 177 181 4 827.40 2.12 502 178 181 3 854.74 1.55 503 178 182 4 837.88 7.36 504 178 219 41 119.53 1.17 505 178 220 42 119.50 1.17 506 178 223 45 119.37 1.16 507 179 182 3 870.26 4.57 508 179 183 4 839.80 6.41 509 180 183 3 847.36 2.65 510 180 184 4 842.75 4.96 511 181 184 3 863.69 0.33 512 181 185 4 863.33 2.75 513 181 208 27 207.41 0.47 514 181 220 39 119.50 1.18 515 181 226 45 119.17 1.10 516 182 185 3 863.72 2.56 517 182 186 4 853.10 2.37 518 182 223 41 119.20 1.10 519 182 224 42 119.20 1.10 520 182 225 43 119.12 1.13 521 182 226 44 119.11 1.12 522 183 186 3 878.70 0.53 523 183 187 4 863.34 2.76 524 184 187 3 866.31 1.88 525 184 188 4 853.09 2.37 526 185 188 3 863.93 3.06 527 185 189 4 845.29 6.25 528 185 206 21 207.61 0.53 529 185 207 22 207.59 0.53 530 185 208 23 207.56 0.54 531 185 225 40 118.89 1.17 532 185 226 41 118.82 1.20 533 186 189 3 838.72 0.51 534 186 190 4 827.94 4.87 535 186 225 39 118.83 1.20 536 187 190 3 845.93 4.12 537 187 191 4 831.27 3.20 538 188 191 3 843.18 9.14 539 188 192 4 820.37 2.26 540 188 206 18 207.59 0.52 541 189 193 4 826.62 0.85 542 189 201 12 208.14 0.48 543 189 204 15 207.88 0.56 544 189 206 17 207.64 0.55 545 189 225 36 118.82 1.20 546 189 227 38 118.81 1.19 547 190 193 3 852.48 2.96 548 190 194 4 854.57 4.00 549 191 194 3 876.90 3.43 550 192 195 3 845.74 7.06 551 192 196 4 695.91 52.20 552 192 197 5 695.89 52.22 553 192 204 12 208.06 0.46 554 193 197 4 496.68 45.20 555 193 198 5 229.87 10.28 556 193 201 8 208.19 0.48 557 196 200 4 693.30 63.22 558 196 204 8 264.94 0.94 559 197 200 3 870.08 3.97 560 197 201 4 825.60 6.04 561 198 201 3 863.47 4.22 562 198 202 4 825.61 6.04 563 199 202 3 857.62 1.03 564 199 203 4 840.82 3.68 565 200 203 3 853.31 2.18 566 200 204 4 825.57 6.03 567 201 204 3 838.07 4.00 568 205 227 22 117.95 1.09 569 206 225 19 118.85 1.19 570 206 226 20 118.81 1.19 571 206 227 21 118.76 1.19 572 207 227 20 118.81 1.19 573 207 228 21 117.94 1.09 574 207 229 22 117.91 1.12 575 207 230 23 117.87 1.12 576 208 212 4 855.31 1.46 577 208 220 12 119.42 1.14 578 208 226 18 119.01 1.11 579 208 228 20 118.81 1.19 580 208 229 21 118.37 1.17 581 208 230 22 118.10 1.03 582 209 230 21 118.28 1.12 583 211 216 5 859.04 0.42 584 212 216 4 855.46 2.21 585 212 229 17 119.15 1.07 586 212 231 19 118.84 1.20 587 213 216 3 871.07 1.68 588 213 217 4 854.22 3.09 589 213 239 26 57.81 1.18 590 213 243 30 56.22 1.52 591 214 217 3 872.11 2.52 592 214 239 25 57.78 1.17 593 214 242 28 56.95 1.45 594 214 243 29 56.22 1.51 595 215 218 3 907.40 1.73 596 216 219 3 874.33 0.52 597 216 220 4 874.33 0.52 598 217 220 3 876.27 0.46 599 217 229 12 119.35 1.10 600 217 243 26 53.06 0.97 601 217 246 29 44.80 0.81 602 217 248 31 38.39 4.00 603 218 221 3 863.25 5.02 604 218 246 28 40.70 2.81 605 220 223 3 870.25 0.51 606 220 226 6 119.73 1.28 607 220 229 9 119.28 1.13 608 220 248 28 33.52 3.74 609 221 246 25 37.12 3.40 610 221 247 26 34.99 3.58 611 221 248 27 24.32 0.78 612 223 226 3 886.45 6.22 613 224 227 3 927.99 1.96 614 226 248 22 19.98 0.59 615 229 243 14 48.75 0.48 616 229 248 19 21.21 0.03 617 231 234 3 899.14 0.23 618 231 235 4 830.05 0.25 619 231 240 9 57.80 1.19 620 231 243 12 54.13 0.98 621 232 235 3 865.21 1.41 622 233 236 3 876.78 0.63 623 233 237 4 866.09 4.71 624 234 237 3 852.41 0.33 625 234 239 5 449.99 32.96 626 234 240 6 242.01 12.04 627 235 240 5 267.22 0.56 628 237 240 3 867.48 3.73 629 238 241 3 878.40 1.73 630 239 242 3 862.71 6.96 631 239 243 4 858.77 1.64 632 240 243 3 869.37 3.63 633 240 244 4 858.82 1.65 634 241 244 3 858.91 1.60 635 241 245 4 855.02 0.24 636 242 245 3 863.17 3.62 637 242 246 4 855.02 0.24 638 243 246 3 874.12 6.00