BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 16 139 137 351.93 0.12 2 16 140 138 351.93 0.12 3 16 142 140 354.74 1.27 4 16 143 141 359.19 0.86 5 16 144 142 359.76 0.58 6 16 156 154 352.25 0.04 7 16 157 155 352.25 0.04 8 16 158 156 351.30 0.42 9 16 189 191 98.66 1.41 10 16 190 192 79.98 4.03 11 16 194 196 79.98 4.03 12 17 143 140 359.88 0.59 13 17 144 141 359.83 0.60 14 17 145 142 361.03 0.66 15 17 146 143 361.08 0.67 16 17 187 188 101.14 1.48 17 17 188 189 100.66 1.72 18 17 189 190 100.49 1.75 19 17 191 192 100.54 1.77 20 17 220 220 100.55 1.77 21 17 221 221 100.56 1.76 22 18 156 152 364.52 1.46 23 18 188 188 101.14 1.48 24 19 157 152 359.77 0.59 25 19 158 153 351.80 0.19 26 19 188 187 101.42 1.44 27 20 156 150 366.06 0.78 28 20 157 151 361.09 0.77 29 21 144 137 380.66 2.60 30 21 152 145 380.79 2.59 31 21 154 147 381.81 2.06 32 21 155 148 380.66 2.58 33 21 156 149 366.56 0.53 34 22 26 4 869.30 4.26 35 22 27 5 864.23 3.50 36 22 154 146 388.61 0.54 37 22 155 147 381.75 2.07 38 22 156 148 380.62 2.59 39 22 157 149 377.61 1.09 40 23 26 3 908.82 0.85 41 23 27 4 869.31 4.26 42 23 71 56 545.33 5.54 43 23 154 145 390.81 0.32 44 24 27 3 886.45 4.32 45 24 28 4 857.81 0.29 46 24 30 6 825.09 5.75 47 24 69 53 545.34 5.51 48 24 70 54 545.40 5.55 49 24 71 55 545.44 5.54 50 24 77 61 564.20 3.62 51 24 117 107 531.26 1.37 52 24 155 145 390.80 0.32 53 25 28 3 895.06 0.01 54 25 116 105 531.94 1.53 55 25 117 106 531.30 1.36 56 27 30 3 893.96 8.32 57 27 137 124 390.50 0.38 58 27 139 126 393.31 1.75 59 27 155 142 407.59 0.43 60 27 157 144 393.37 1.78 61 28 116 102 532.56 1.45 62 28 117 103 532.47 1.49 63 28 118 104 532.29 1.44 64 28 119 105 532.27 1.45 65 29 45 20 661.46 26.31 66 29 46 21 655.60 28.59 67 29 119 104 532.29 1.44 68 29 120 105 532.26 1.45 69 29 121 106 532.26 1.45 70 29 137 122 411.98 2.32 71 29 198 187 265.27 0.17 72 29 200 189 251.31 0.09 73 29 207 196 239.84 0.04 74 30 40 14 671.65 27.16 75 30 43 17 664.51 24.89 76 30 44 18 663.73 25.29 77 30 68 46 692.29 0.35 78 30 139 123 409.30 0.39 79 30 140 124 411.32 0.65 80 30 141 125 411.76 0.66 81 30 155 139 411.70 0.64 82 30 197 185 265.69 0.18 83 30 198 186 265.59 0.18 84 31 43 16 664.58 24.87 85 31 44 17 663.73 25.29 86 31 45 18 663.93 25.18 87 31 52 25 663.28 25.07 88 31 66 43 693.55 0.99 89 31 67 44 692.29 0.35 90 31 68 45 692.29 0.35 91 32 40 12 671.62 27.17 92 32 66 42 693.63 0.96 93 32 67 43 692.31 0.36 94 32 141 123 413.50 0.20 95 33 40 11 671.65 27.16 96 33 41 12 671.58 27.18 97 33 42 13 670.89 27.05 98 33 43 14 667.52 25.89 99 33 44 15 667.45 25.91 100 33 52 23 664.50 24.47 101 33 54 25 664.48 24.42 102 33 59 30 665.14 23.95 103 33 64 39 695.19 1.73 104 33 65 40 693.71 0.99 105 33 66 41 693.69 0.97 106 33 106 83 616.12 0.26 107 34 38 8 716.12 5.60 108 34 39 9 684.30 20.90 109 34 40 10 672.28 26.85 110 34 41 11 671.62 27.17 111 34 64 38 695.22 1.74 112 34 65 39 694.53 1.40 113 34 67 41 693.82 0.93 114 34 70 44 694.38 0.66 115 34 73 47 693.60 0.21 116 35 38 7 724.71 1.28 117 35 39 8 715.68 5.38 118 35 41 10 684.29 20.91 119 35 60 31 692.63 10.03 120 35 63 36 695.53 1.90 121 35 64 37 695.50 1.87 122 36 37 5 731.60 2.71 123 36 38 6 724.71 1.28 124 36 62 34 695.69 1.89 125 36 63 35 695.54 1.90 126 36 65 37 695.64 1.83 127 36 84 56 692.69 0.27 128 37 37 3 887.50 5.50 129 37 37 4 763.63 14.16 130 37 39 6 750.62 7.65 131 38 65 31 696.38 1.46 132 40 73 37 694.00 0.01 133 40 141 111 415.59 0.52 134 40 151 121 415.55 0.47 135 40 193 167 269.90 0.78 136 41 58 17 703.18 4.94 137 41 59 18 703.21 4.92 138 41 64 27 696.43 1.43 139 42 58 16 703.20 4.92 140 42 195 167 269.78 0.81 141 43 54 11 703.32 5.00 142 43 195 166 269.73 0.83 143 43 196 167 266.69 0.07 144 43 197 168 265.67 0.18 145 44 52 8 724.45 5.50 146 44 53 9 706.60 3.40 147 44 54 10 703.14 4.90 148 44 196 166 267.12 0.29 149 44 197 167 265.81 0.23 150 45 52 7 724.46 5.50 151 45 53 8 706.61 3.40 152 45 121 86 532.93 1.27 153 45 198 167 265.69 0.18 154 45 209 178 239.75 0.02 155 46 49 3 944.60 3.98 156 46 51 5 776.19 4.35 157 46 52 6 724.46 5.50 158 46 68 26 693.75 0.40 159 46 108 68 691.12 1.10 160 46 112 76 534.79 1.57 161 46 118 82 533.52 1.29 162 46 119 83 533.38 1.27 163 46 120 84 533.28 1.28 164 46 121 85 533.12 1.34 165 47 51 4 834.22 2.48 166 47 53 6 743.61 0.50 167 47 105 64 691.25 1.11 168 47 120 83 533.36 1.26 169 47 121 84 533.16 1.34 170 47 123 86 532.91 1.28 171 47 209 176 239.65 0.03 172 47 238 205 110.89 0.29 173 48 51 3 869.34 0.19 174 48 120 82 533.44 1.26 175 49 108 65 691.20 1.11 176 49 111 72 535.03 1.61 177 49 112 73 534.85 1.54 178 49 120 81 533.46 1.25 179 50 107 63 691.24 1.12 180 50 108 64 691.14 1.11 181 50 111 71 535.23 1.70 182 51 89 42 693.19 0.68 183 51 105 60 691.37 1.18 184 51 106 61 691.28 1.15 185 51 107 62 691.22 1.14 186 51 108 63 691.21 1.14 187 51 241 204 93.42 1.77 188 51 242 205 81.31 0.46 189 51 244 207 80.39 0.00 190 52 66 18 694.95 0.09 191 52 105 59 691.38 1.20 192 52 106 60 691.26 1.17 193 52 108 62 691.24 1.16 194 53 103 56 691.60 1.29 195 53 104 57 691.44 1.23 196 53 105 58 691.38 1.22 197 53 209 170 239.88 0.03 198 53 212 173 236.52 0.66 199 53 238 199 110.50 0.47 200 54 59 5 880.69 0.47 201 54 103 55 691.60 1.30 202 54 104 56 691.47 1.26 203 54 106 58 691.43 1.25 204 54 196 156 269.50 0.88 205 54 212 172 236.53 0.66 206 55 58 3 905.99 2.17 207 55 59 4 895.58 2.79 208 55 102 53 691.63 1.33 209 55 103 54 691.59 1.31 210 55 104 55 691.57 1.32 211 55 196 155 269.68 0.85 212 55 212 171 238.86 0.01 213 55 213 172 236.77 0.64 214 56 59 3 907.23 3.04 215 56 60 4 876.81 1.46 216 56 90 38 692.49 1.07 217 56 91 39 692.48 1.07 218 56 94 42 692.07 1.26 219 56 102 52 691.66 1.35 220 56 103 53 691.61 1.34 221 56 104 54 690.96 1.64 222 57 60 3 886.67 2.35 223 57 94 41 692.08 1.26 224 57 99 48 691.75 1.37 225 57 102 51 691.66 1.35 226 57 195 152 270.12 0.87 227 57 214 171 236.68 0.58 228 58 195 151 269.98 0.83 229 58 196 152 269.82 0.81 230 59 60 3 875.78 0.69 231 59 60 4 812.82 0.58 232 59 64 9 727.61 0.47 233 59 88 33 693.86 0.80 234 59 90 35 692.55 1.10 235 59 104 51 688.06 3.12 236 59 106 53 666.21 14.01 237 60 60 3 875.12 1.02 238 60 90 34 692.92 0.91 239 60 94 38 692.03 1.29 240 60 64 6 839.12 0.01 241 60 88 29 694.03 0.78 242 60 90 31 693.85 0.84 243 61 63 3 899.68 1.94 244 61 64 4 885.81 2.22 245 62 64 3 899.68 1.94 246 62 85 24 695.06 0.95 247 62 86 25 695.06 0.95 248 62 87 26 694.74 1.05 249 62 88 27 694.44 1.03 250 64 85 21 695.00 1.02 251 64 88 24 694.59 1.20 252 65 82 17 697.24 1.93 253 65 83 18 696.94 1.83 254 66 82 16 697.24 1.93 255 66 83 17 696.97 1.83 256 66 85 19 696.95 1.84 257 66 106 42 615.82 0.41 258 66 108 44 610.96 0.37 259 67 70 3 909.47 4.76 260 67 80 13 697.68 2.02 261 67 81 14 697.50 2.03 262 67 82 15 697.38 2.00 263 68 80 12 697.69 2.02 264 68 81 13 697.63 2.02 265 68 83 15 697.57 1.99 266 68 108 42 611.59 0.61 267 68 112 50 533.85 1.33 268 68 118 56 533.38 1.27 269 69 77 8 716.98 11.46 270 69 80 11 697.69 2.02 271 69 117 54 533.37 1.28 272 69 118 55 533.35 1.27 273 70 73 3 1016.77 2.81 274 70 74 4 940.63 2.91 275 70 75 5 884.11 4.20 276 70 76 6 784.51 9.56 277 70 77 7 716.97 11.46 278 70 80 10 697.77 2.06 279 71 77 6 783.16 10.22 280 71 141 76 415.43 0.58 281 71 154 89 414.17 0.04 282 71 155 90 411.57 0.70 283 72 75 3 895.91 1.42 284 72 77 5 812.06 0.58 285 72 141 75 415.49 0.56 286 72 153 87 415.08 0.27 287 72 154 88 414.17 0.04 288 73 141 74 415.47 0.57 289 73 151 84 415.53 0.48 290 73 152 85 415.50 0.47 291 73 153 86 415.23 0.34 292 74 153 85 415.49 0.47 293 75 153 84 415.49 0.47 294 76 80 4 864.88 1.51 295 77 80 3 877.40 0.97 296 79 118 45 533.50 1.24 297 81 118 43 533.47 1.25 298 82 110 30 607.09 1.44 299 83 108 27 612.34 0.30 300 83 109 28 607.24 1.39 301 83 110 29 607.09 1.43 302 83 111 34 535.37 1.74 303 83 112 35 535.12 1.77 304 84 108 26 614.40 0.72 305 84 109 27 610.35 0.03 306 84 110 29 607.12 1.44 307 85 88 3 920.38 2.95 308 85 106 23 616.48 0.42 309 85 107 24 616.12 0.26 310 86 107 23 616.16 0.27 311 86 109 25 615.66 0.46 312 87 106 21 619.39 1.75 313 87 107 22 617.69 1.00 314 88 105 19 624.58 3.74 315 88 106 20 618.91 1.16 316 89 104 17 626.32 3.08 317 89 105 18 625.36 3.35 318 89 237 158 119.36 1.59 319 89 241 162 93.79 4.50 320 90 104 16 626.35 3.06 321 90 237 157 121.95 0.30 322 91 94 3 876.24 2.84 323 91 101 12 690.60 1.88 324 91 103 14 679.63 7.29 325 91 234 153 150.83 0.12 326 91 237 156 121.95 0.30 327 93 101 10 691.89 1.48 328 94 99 7 748.11 9.27 329 94 100 8 702.12 6.54 330 94 101 9 691.89 1.48 331 94 102 10 691.88 1.48 332 95 99 6 748.12 9.27 333 95 100 7 703.77 7.37 334 96 98 4 820.39 2.57 335 96 99 5 762.77 12.68 336 97 98 3 877.51 7.22 337 97 100 5 841.22 3.60 338 97 172 81 287.44 0.54 339 97 175 84 284.74 1.23 340 97 215 126 239.83 0.04 341 97 217 128 239.15 0.10 342 98 175 83 284.35 1.27 343 98 180 88 283.88 1.19 344 98 215 125 239.81 0.04 345 99 102 3 904.17 4.30 346 99 180 87 283.82 1.18 347 99 214 123 239.82 0.05 348 99 215 124 239.56 0.04 349 100 177 83 284.01 1.11 350 100 180 86 283.59 1.10 351 101 179 84 283.25 1.06 352 101 234 141 151.19 0.27 353 102 214 120 236.77 0.64 354 102 229 135 168.47 0.53 355 103 212 117 239.63 0.02 356 103 229 134 168.44 0.52 357 103 234 139 150.02 0.21 358 103 237 142 119.33 1.61 359 103 238 143 115.50 0.04 360 104 237 141 119.33 1.61 361 105 237 140 119.30 1.59 362 105 238 141 111.24 0.20 363 105 241 144 83.14 0.83 364 105 242 145 81.35 0.44 365 107 244 145 80.01 0.18 366 108 111 7 535.37 1.74 367 108 112 8 535.37 1.74 368 109 110 4 826.64 11.29 369 109 110 5 766.11 18.98 370 110 110 3 869.18 4.08 371 110 110 4 793.27 8.49 372 112 118 6 824.23 0.06 373 113 118 5 827.43 1.53 374 114 118 4 824.25 0.07 375 115 118 3 895.81 0.64 376 115 119 4 856.90 2.28 377 116 119 3 955.00 1.35 378 121 200 83 258.23 0.18 379 121 207 90 239.86 0.03 380 121 208 91 239.75 0.03 381 121 209 92 239.35 0.10 382 122 206 88 240.06 0.04 383 122 207 89 239.99 0.01 384 122 208 90 239.80 0.05 385 122 209 91 239.65 0.02 386 123 208 89 239.88 0.04 387 123 209 90 239.66 0.02 388 123 231 112 152.42 0.60 389 123 235 116 129.89 0.67 390 123 238 119 113.69 0.86 391 124 208 88 240.00 0.01 392 124 209 89 239.89 0.04 393 124 231 111 155.05 0.70 394 124 232 112 150.94 0.12 395 124 235 115 140.52 2.89 396 125 128 3 879.14 2.16 397 125 232 111 152.41 0.61 398 125 235 114 142.58 1.92 399 126 235 113 148.73 0.29 400 126 236 114 148.44 0.42 401 127 232 109 155.12 0.67 402 127 233 110 155.11 0.67 403 128 203 79 265.87 0.26 404 128 204 80 265.86 0.26 405 128 232 108 155.12 0.67 406 129 203 78 266.41 0.01 407 129 210 85 240.19 0.01 408 129 230 105 168.29 0.46 409 129 232 107 155.30 0.56 410 130 134 4 851.34 0.77 411 130 162 32 411.63 0.67 412 130 201 75 266.99 0.10 413 130 203 77 266.41 0.01 414 130 210 84 240.21 0.01 415 131 134 3 895.81 1.99 416 132 162 30 411.63 0.66 417 132 163 31 411.49 0.59 418 132 164 32 411.12 0.74 419 133 161 28 411.78 0.69 420 133 162 29 411.71 0.66 421 134 161 27 413.46 0.14 422 134 162 28 411.78 0.69 423 135 159 24 414.81 0.06 424 135 160 25 414.71 0.04 425 135 161 26 413.81 0.21 426 136 159 23 414.88 0.02 427 136 160 24 414.79 0.07 428 136 162 26 414.20 0.21 429 136 199 67 266.58 0.01 430 136 200 68 266.49 0.03 431 136 201 69 264.50 0.71 432 137 158 21 415.22 0.14 433 137 159 22 414.88 0.02 434 137 199 66 266.49 0.03 435 137 200 67 266.25 0.01 436 137 207 74 240.19 0.01 437 138 157 19 415.33 0.18 438 138 158 20 415.31 0.18 439 138 160 22 415.19 0.15 440 138 189 55 270.43 0.86 441 138 190 56 270.33 0.91 442 138 198 64 266.49 0.03 443 138 199 65 266.30 0.03 444 138 228 94 169.19 0.89 445 139 155 16 415.70 0.17 446 139 156 17 415.45 0.20 447 139 157 18 415.33 0.18 448 139 198 63 266.47 0.03 449 140 155 15 415.69 0.16 450 140 156 16 415.66 0.17 451 140 194 58 269.81 0.83 452 141 151 10 417.57 0.45 453 141 152 11 417.43 0.46 454 141 153 12 417.24 0.39 455 141 154 13 416.79 0.58 456 141 155 14 415.62 0.12 457 141 194 57 269.84 0.82 458 142 151 9 417.60 0.44 459 142 152 10 417.59 0.45 460 142 192 54 270.18 0.91 461 142 193 55 269.89 0.82 462 142 194 56 269.84 0.81 463 143 146 3 916.51 5.64 464 143 148 5 796.79 12.71 465 143 149 6 752.02 6.90 466 143 151 8 417.61 0.44 467 143 152 9 417.61 0.44 468 143 191 52 270.25 0.95 469 143 192 53 270.18 0.92 470 144 148 4 872.55 6.36 471 144 152 8 417.73 0.38 472 144 156 12 416.88 0.57 473 145 148 3 877.26 4.00 474 146 220 77 184.84 0.24 475 151 192 45 270.19 0.92 476 151 193 46 269.89 0.82 477 152 156 4 895.21 4.65 478 158 188 34 270.50 0.93 479 159 188 33 270.56 0.92 480 160 183 25 271.81 0.88 481 160 184 26 271.25 0.96 482 160 185 27 271.16 0.96 483 160 188 32 270.62 0.89 484 160 189 33 270.55 0.91 485 160 199 43 267.48 0.14 486 161 183 24 271.84 0.86 487 161 184 25 271.71 0.92 488 161 185 27 271.48 0.83 489 162 181 21 272.29 0.87 490 162 182 22 272.18 0.84 491 162 183 23 272.03 0.78 492 162 199 41 269.86 0.82 493 162 201 43 268.83 1.00 494 163 167 4 860.93 0.34 495 163 168 5 857.56 1.88 496 163 182 21 272.27 0.87 497 163 183 22 272.13 0.82 498 163 184 23 272.13 0.82 499 163 225 66 170.40 0.53 500 164 167 3 897.10 6.87 501 164 168 4 860.79 0.27 502 165 168 3 878.07 1.60 503 165 169 4 868.91 4.33 504 165 176 13 283.33 0.61 505 165 177 14 283.32 0.61 506 165 178 15 283.05 0.58 507 166 169 3 890.32 3.00 508 166 170 4 880.94 1.68 509 167 170 3 898.85 1.26 510 167 171 6 805.64 8.92 511 168 171 5 791.06 2.30 512 168 172 6 789.47 3.10 513 168 176 10 582.47 78.82 514 168 182 16 272.32 0.85 515 168 225 61 176.61 0.49 516 169 173 6 731.62 4.33 517 169 174 7 731.60 4.34 518 169 176 9 584.66 77.73 519 170 171 3 854.52 5.96 520 170 172 4 833.97 8.00 521 170 173 5 775.94 3.61 522 170 173 4 776.87 3.20 523 170 223 55 185.09 0.23 524 170 224 56 185.08 0.22 525 171 186 17 272.22 0.87 526 171 223 54 185.02 0.23 527 171 224 55 184.96 0.24 528 171 225 56 184.96 0.24 529 172 175 3 896.90 0.02 530 172 176 4 858.92 1.29 531 172 215 45 239.90 0.01 532 172 216 46 239.69 0.01 533 172 217 47 238.28 0.48 534 172 224 54 184.96 0.23 535 172 225 55 184.96 0.23 536 172 227 57 180.98 0.85 537 173 176 3 927.06 6.90 538 175 180 5 868.23 3.35 539 176 180 4 848.30 2.41 540 176 182 6 816.17 14.25 541 176 215 41 239.86 0.03 542 176 227 53 170.23 0.48 543 177 180 3 882.16 7.79 544 178 230 54 169.89 0.54 545 178 233 57 168.48 0.53 546 179 230 53 168.61 0.53 547 179 233 56 160.40 0.49 548 179 234 57 162.33 1.90 549 180 215 37 239.41 0.09 550 180 227 49 170.01 0.49 551 180 228 50 169.97 0.49 552 180 230 52 168.50 0.52 553 181 199 20 269.99 0.85 554 181 210 31 240.18 0.00 555 181 227 48 170.01 0.50 556 181 228 49 169.93 0.52 557 181 230 51 168.44 0.52 558 182 225 45 170.40 0.53 559 182 226 46 170.27 0.50 560 182 227 47 170.01 0.50 561 183 199 18 269.82 0.81 562 183 225 44 170.39 0.54 563 183 226 45 170.10 0.50 564 183 228 47 170.01 0.50 565 184 186 4 855.72 3.89 566 184 225 43 170.39 0.54 567 185 186 3 855.72 3.89 568 185 187 4 855.72 3.89 569 185 188 5 741.00 0.42 570 186 221 34 185.01 0.24 571 186 222 35 185.00 0.24 572 186 223 36 184.78 0.32 573 186 223 37 184.78 0.33 574 186 224 38 184.61 0.39 575 186 225 39 177.69 0.86 576 187 221 33 185.03 0.23 577 188 221 32 185.00 0.21 578 189 220 30 185.03 0.23 579 189 221 31 184.88 0.26 580 189 226 37 170.34 0.55 581 190 194 4 830.76 5.04 582 190 213 23 236.47 0.65 583 190 220 29 185.00 0.23 584 190 228 38 170.05 0.50 585 191 194 3 902.44 7.88 586 191 220 28 184.87 0.26 587 192 220 27 184.99 0.23 588 194 197 3 893.62 0.87 589 194 213 19 235.55 0.52 590 195 213 18 235.56 0.52 591 196 212 16 236.52 0.66 592 196 213 17 235.86 0.36 593 197 212 15 235.66 0.47 594 197 213 16 234.19 1.05 595 198 209 11 239.81 0.05 596 198 211 13 237.31 0.81 597 199 209 10 239.86 0.02 598 199 210 11 239.74 0.03 599 199 211 12 236.72 0.58 600 199 228 29 168.47 0.53 601 200 207 7 240.16 0.01 602 200 208 8 239.88 0.03 603 200 209 9 239.78 0.04 604 201 206 5 816.72 4.53 605 201 207 6 240.22 0.01 606 201 208 7 240.13 0.00 607 201 210 9 239.68 0.06 608 202 206 4 780.73 10.09 609 202 207 5 264.76 12.26 610 203 206 3 870.91 2.42 611 203 208 5 516.62 113.66 612 209 212 3 1001.46 1.80 613 209 231 22 152.41 0.59 614 210 230 20 157.40 0.24 615 210 231 21 152.44 0.60 616 211 228 17 168.46 0.52 617 211 229 18 168.26 0.48 618 211 231 20 152.42 0.60 619 212 228 16 168.47 0.53 620 212 229 17 168.22 0.49 621 212 231 19 152.39 0.59 622 213 228 15 168.53 0.56 623 214 227 13 170.01 0.50 624 214 228 14 169.40 0.76 625 214 229 15 168.62 0.52 626 215 226 11 170.38 0.54 627 215 227 12 170.05 0.48 628 216 227 11 170.34 0.53 629 217 222 4 881.64 0.69 630 217 224 7 181.72 0.74 631 219 222 3 905.91 4.25 632 222 224 3 859.04 0.33 633 229 234 5 870.13 2.15 634 230 233 3 898.75 7.83 635 230 234 4 880.84 2.40 636 231 234 3 894.74 3.13 637 231 235 4 870.37 2.04 638 231 238 7 851.25 7.01 639 232 235 3 898.87 1.06 640 234 237 3 867.81 0.76 641 234 238 4 865.38 0.33 642 235 238 3 865.99 0.25 643 235 239 4 865.47 0.29 644 236 239 3 868.82 1.66 645 236 240 4 865.75 0.14 646 237 240 3 879.58 3.13 647 237 241 4 865.50 0.01 648 238 241 3 879.58 3.13 649 238 242 4 865.59 0.05 650 239 242 3 876.03 4.90 651 239 243 4 865.85 0.19 652 240 243 3 886.04 0.12 653 241 244 3 900.34 0.82