BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 203 200 111.78 1.15 2 3 204 201 111.53 1.26 3 3 205 202 111.18 1.41 4 3 227 224 110.98 1.40 5 4 34 30 744.66 35.36 6 4 35 31 744.65 35.34 7 4 36 32 744.62 35.34 8 4 37 33 744.61 35.34 9 4 38 34 744.61 35.34 10 4 205 201 111.56 1.24 11 5 37 32 744.62 35.34 12 5 38 33 744.62 35.34 13 5 221 216 111.20 1.40 14 5 226 221 111.53 1.26 15 5 227 222 111.45 1.27 16 5 247 242 19.73 1.02 17 6 37 31 744.64 35.34 18 6 38 32 744.61 35.34 19 6 40 34 744.61 35.34 20 6 90 84 153.25 18.88 21 6 124 118 113.12 1.20 22 6 161 155 113.03 1.18 23 6 205 199 112.73 1.27 24 6 207 201 111.52 1.26 25 6 227 221 111.54 1.27 26 6 228 222 111.19 1.41 27 6 229 223 111.11 1.44 28 7 37 30 744.69 35.34 29 7 38 31 744.64 35.35 30 7 39 32 744.62 35.34 31 7 40 33 744.62 35.34 32 7 229 222 111.19 1.41 33 7 240 233 52.42 0.30 34 7 243 236 43.23 0.80 35 7 244 237 37.13 0.42 36 7 247 240 25.61 1.15 37 8 40 32 744.62 35.34 38 8 42 34 744.61 35.34 39 8 229 221 111.81 1.15 40 8 230 222 111.13 1.44 41 8 231 223 111.11 1.44 42 8 240 232 52.48 0.32 43 9 40 31 744.64 35.34 44 9 41 32 744.61 35.34 45 9 42 33 744.61 35.34 46 9 231 222 111.47 1.27 47 9 235 226 82.34 0.61 48 9 240 231 52.48 0.32 49 10 42 32 744.62 35.34 50 10 64 54 357.66 25.53 51 10 92 82 156.61 17.23 52 10 95 85 153.84 15.92 53 10 230 220 112.99 1.24 54 10 231 221 111.98 1.04 55 10 232 222 111.08 1.46 56 10 235 225 86.83 0.54 57 11 14 3 870.60 2.38 58 11 20 9 746.05 35.26 59 11 23 12 745.78 35.38 60 11 27 16 745.59 35.30 61 11 41 30 744.68 35.34 62 11 42 31 744.63 35.34 63 11 43 32 744.62 35.34 64 11 51 40 414.14 0.54 65 11 64 53 357.81 25.48 66 11 235 224 105.44 1.25 67 11 236 225 83.36 0.13 68 12 44 32 744.63 35.34 69 12 64 52 357.69 25.52 70 12 95 83 153.40 18.91 71 12 97 85 164.33 13.36 72 12 170 158 150.46 17.56 73 12 232 220 112.93 1.26 74 13 44 31 744.64 35.34 75 14 44 30 744.66 35.34 76 14 236 222 110.80 1.48 77 15 19 4 767.36 29.77 78 15 20 5 765.23 27.45 79 15 47 32 744.29 35.16 80 16 19 3 852.30 4.66 81 16 20 4 822.86 2.08 82 16 47 31 744.31 35.18 83 17 21 4 831.32 5.17 84 17 50 33 696.98 36.20 85 18 21 3 856.03 2.71 86 18 22 4 843.47 9.54 87 19 22 3 852.26 5.15 88 19 23 4 827.99 1.80 89 20 23 3 851.21 1.89 90 20 24 4 827.06 1.33 91 20 47 27 744.00 35.03 92 20 50 30 657.19 35.06 93 20 51 31 543.17 65.11 94 20 54 34 413.86 0.68 95 21 24 3 858.91 3.69 96 21 25 4 839.52 7.57 97 21 54 33 413.74 0.69 98 22 25 3 862.36 3.84 99 22 26 4 861.06 3.20 100 23 26 3 866.10 1.61 101 23 27 4 858.35 1.84 102 23 235 212 108.17 2.48 103 23 236 213 96.99 0.09 104 23 237 214 84.12 0.74 105 23 238 215 81.52 0.40 106 24 27 3 862.00 3.67 107 24 28 4 858.35 1.84 108 24 54 30 361.33 25.45 109 24 55 31 361.30 25.46 110 25 28 3 862.18 0.06 111 25 29 4 858.35 1.86 112 26 29 3 862.18 0.06 113 26 238 212 76.91 0.93 114 26 241 215 56.65 1.21 115 27 30 3 877.32 0.95 116 27 41 14 744.72 35.30 117 27 55 28 361.12 25.37 118 27 59 32 358.41 25.52 119 27 235 208 97.09 0.04 120 27 237 210 83.94 0.81 121 27 240 213 53.44 0.16 122 27 241 214 52.54 0.28 123 28 54 26 361.14 25.37 124 28 55 27 360.75 25.18 125 28 56 28 360.68 25.21 126 28 58 30 359.76 24.88 127 28 59 31 359.27 25.12 128 30 58 28 359.18 25.16 129 30 241 211 52.40 0.30 130 30 244 214 50.23 0.67 131 30 245 215 46.02 0.20 132 30 248 218 40.38 0.05 133 32 39 7 744.81 35.28 134 32 58 26 358.19 25.52 135 32 59 27 358.21 25.53 136 32 248 216 30.62 0.49 137 33 36 3 847.23 3.81 138 33 37 4 764.58 25.86 139 34 37 3 850.76 2.04 140 34 58 24 357.97 25.52 141 37 244 207 39.09 0.17 142 37 247 210 25.18 1.67 143 37 248 211 19.73 1.02 144 38 205 167 113.09 1.20 145 39 59 20 357.96 25.52 146 39 60 21 357.93 25.52 147 39 244 205 36.94 0.35 148 40 60 20 357.93 25.52 149 40 61 21 357.92 25.53 150 40 62 22 357.86 25.50 151 40 90 50 153.46 18.84 152 41 51 10 360.78 25.20 153 41 55 14 359.04 25.68 154 41 59 18 358.19 25.52 155 41 60 19 358.11 25.55 156 41 61 20 357.91 25.52 157 41 62 21 357.88 25.50 158 42 62 20 357.88 25.50 159 42 63 21 357.86 25.50 160 42 64 22 357.73 25.52 161 42 90 48 156.96 17.40 162 42 91 49 156.87 17.42 163 42 92 50 156.85 17.34 164 42 124 82 156.70 17.41 165 43 47 4 813.78 2.06 166 43 48 5 746.02 35.87 167 43 51 8 412.69 0.17 168 43 62 19 358.11 25.54 169 43 64 21 357.88 25.50 170 44 47 3 855.33 6.54 171 44 48 4 831.46 0.50 172 44 64 20 357.92 25.51 173 45 48 3 861.18 3.62 174 47 50 3 861.04 3.62 175 47 51 4 830.75 3.15 176 48 51 3 854.25 6.83 177 48 52 4 830.76 3.14 178 48 61 13 358.29 25.46 179 48 62 14 358.28 25.46 180 48 63 15 358.18 25.50 181 48 88 40 166.02 13.21 182 49 52 3 859.90 6.32 183 50 53 3 855.18 1.88 184 50 54 4 852.51 0.55 185 51 54 3 871.00 5.29 186 51 55 4 846.96 2.23 187 52 55 3 867.78 6.91 188 52 56 4 866.50 7.54 189 52 57 5 841.66 0.43 190 52 59 7 570.26 53.26 191 52 60 8 401.87 3.78 192 52 61 9 358.33 25.47 193 55 59 4 827.30 3.50 194 55 61 6 412.36 1.47 195 56 59 3 869.74 0.09 196 59 244 185 39.26 0.25 197 60 89 29 153.59 18.78 198 60 90 30 153.45 18.84 199 61 89 28 154.16 18.55 200 61 90 29 153.46 18.84 201 62 88 26 155.06 18.35 202 62 90 28 153.46 18.84 203 63 78 15 168.01 13.38 204 63 83 20 167.06 13.17 205 63 88 25 165.76 13.07 206 63 90 27 156.96 17.39 207 63 91 28 156.83 17.39 208 63 92 29 156.90 17.37 209 64 92 28 156.90 17.37 210 65 92 27 164.59 13.62 211 65 98 33 165.74 13.03 212 65 112 47 167.17 13.08 213 66 78 12 168.32 13.25 214 66 79 13 168.25 13.27 215 66 80 14 167.87 13.39 216 66 91 25 166.49 12.86 217 66 93 27 166.39 12.80 218 66 112 46 167.17 13.08 219 66 116 50 167.22 13.11 220 66 121 55 167.25 13.12 221 67 77 10 168.58 13.24 222 67 78 11 168.49 13.28 223 67 79 12 168.25 13.27 224 67 112 45 167.16 13.09 225 68 108 40 167.17 13.09 226 68 111 43 167.29 13.04 227 68 112 44 167.16 13.09 228 69 74 5 547.57 67.73 229 69 79 10 170.00 13.94 230 70 74 4 887.90 2.43 231 70 79 9 170.02 13.94 232 70 81 11 170.03 13.94 233 71 79 8 170.30 13.83 234 71 81 10 170.06 13.94 235 71 82 11 170.06 13.94 236 74 77 3 859.45 3.00 237 78 82 4 844.84 1.72 238 79 82 3 862.94 3.82 239 79 83 4 844.85 1.73 240 80 83 3 857.97 1.33 241 80 84 4 844.86 1.73 242 80 115 35 197.84 17.37 243 80 116 36 197.27 17.19 244 80 119 39 333.74 43.12 245 80 121 41 316.28 41.86 246 81 84 3 853.74 3.58 247 81 85 4 846.30 1.00 248 81 119 38 333.78 43.13 249 82 85 3 853.73 3.58 250 82 86 4 846.29 1.00 251 83 86 3 863.39 1.25 252 83 87 4 836.28 1.74 253 83 88 5 613.88 32.51 254 83 121 38 355.19 44.43 255 84 87 3 839.71 2.23 256 84 119 35 378.23 52.12 257 84 120 36 376.53 52.99 258 84 121 37 367.86 48.68 259 88 121 33 425.74 58.97 260 89 121 32 425.75 58.97 261 89 122 33 425.74 58.97 262 90 122 32 425.74 58.97 263 90 124 34 425.73 58.97 264 90 161 71 425.72 58.97 265 91 116 25 426.08 58.91 266 91 121 30 426.01 58.93 267 91 122 31 425.97 58.92 268 91 123 32 425.74 58.98 269 91 124 33 425.74 58.98 270 92 95 3 912.41 4.38 271 92 124 32 425.75 58.97 272 92 126 34 425.75 58.97 273 92 230 138 165.16 23.98 274 93 109 16 426.25 58.86 275 93 112 19 426.31 58.86 276 93 113 20 426.29 58.87 277 93 116 23 426.30 58.86 278 93 123 30 426.04 58.92 279 93 124 31 425.99 58.91 280 93 125 32 425.89 58.91 281 93 126 33 425.90 58.90 282 94 99 5 760.50 22.34 283 94 104 10 426.30 58.86 284 94 109 15 426.29 58.85 285 94 126 32 425.93 58.89 286 95 98 3 859.74 0.36 287 95 99 4 841.88 3.04 288 95 126 31 426.01 58.90 289 95 165 70 425.95 58.87 290 95 230 135 167.14 24.52 291 96 99 3 851.90 1.96 292 96 100 4 783.34 26.23 293 96 165 69 425.98 58.86 294 96 167 71 423.03 57.41 295 96 170 74 401.41 46.63 296 97 100 3 851.88 1.97 297 97 101 4 781.99 25.55 298 97 102 5 774.52 29.28 299 97 170 73 409.95 50.85 300 98 102 4 706.13 4.88 301 98 103 5 636.72 39.59 302 98 104 6 426.82 59.01 303 99 104 5 426.69 58.94 304 99 106 7 426.83 59.01 305 99 109 10 426.69 58.94 306 99 126 27 426.27 58.84 307 99 127 28 426.25 58.84 308 99 146 47 426.53 58.87 309 103 108 5 851.87 9.69 310 104 108 4 838.48 3.12 311 104 109 5 838.60 3.06 312 104 112 8 768.20 28.11 313 105 108 3 860.41 5.44 314 105 109 4 851.91 9.68 315 106 109 3 859.08 6.11 316 106 110 4 857.16 7.07 317 106 145 39 428.52 58.10 318 106 146 40 426.78 58.95 319 106 149 43 428.53 58.10 320 107 110 3 867.59 1.86 321 107 111 4 867.57 1.85 322 108 111 3 867.95 1.67 323 108 112 4 854.30 4.78 324 109 112 3 859.53 2.16 325 109 113 4 854.32 4.77 326 109 125 16 426.97 58.89 327 109 150 41 438.58 53.34 328 110 113 3 851.83 2.58 329 110 114 4 845.73 0.47 330 110 149 39 438.57 53.34 331 110 150 40 438.57 53.34 332 110 152 42 549.32 1.47 333 110 153 43 549.41 1.50 334 111 114 3 855.89 5.55 335 111 115 4 843.88 0.46 336 112 115 3 858.22 5.07 337 112 116 4 838.73 2.63 338 113 116 3 843.86 0.07 339 113 117 4 838.69 2.65 340 113 123 10 549.06 1.56 341 113 125 12 438.38 53.24 342 113 150 37 442.42 51.73 343 113 153 40 554.94 1.20 344 113 154 41 554.95 1.20 345 113 160 47 549.40 1.51 346 114 117 3 865.68 0.86 347 114 118 4 844.19 0.10 348 114 153 39 554.68 1.07 349 115 118 3 862.46 1.76 350 115 119 4 850.82 3.22 351 116 119 3 856.15 0.55 352 116 120 4 847.83 4.71 353 116 121 5 622.28 23.22 354 116 123 7 558.49 2.99 355 117 120 3 844.22 0.09 356 117 121 4 781.78 30.88 357 117 153 36 598.03 2.62 358 117 154 37 598.04 2.62 359 117 155 38 651.35 29.28 360 117 160 43 570.28 2.83 361 122 159 37 733.72 34.02 362 123 159 36 733.83 34.00 363 123 160 37 733.73 34.01 364 123 161 38 733.75 34.01 365 124 161 37 733.75 34.01 366 124 163 39 733.74 33.98 367 125 150 25 733.97 34.00 368 125 160 35 733.90 33.98 369 125 161 36 733.87 33.98 370 125 162 37 733.79 33.99 371 125 163 38 733.79 33.99 372 126 163 37 733.79 33.99 373 126 165 39 733.73 33.97 374 126 230 104 167.17 24.55 375 127 143 16 734.41 33.95 376 127 146 19 734.28 33.96 377 127 147 20 734.27 33.95 378 127 150 23 734.25 33.97 379 127 162 35 733.90 33.98 380 127 163 36 733.85 33.98 381 127 164 37 733.81 33.99 382 127 165 38 733.81 33.99 383 127 188 61 444.00 29.46 384 128 143 15 734.42 33.95 385 128 146 18 734.38 33.97 386 128 164 36 733.83 33.98 387 128 165 37 733.81 33.99 388 128 166 38 733.78 33.99 389 128 167 39 733.63 33.98 390 129 133 4 767.21 30.87 391 129 134 5 767.18 30.85 392 129 139 10 734.51 33.98 393 129 142 13 734.49 33.97 394 129 143 14 734.50 33.98 395 129 164 35 733.88 33.99 396 129 166 37 733.81 33.99 397 129 167 38 733.79 33.99 398 129 168 39 733.79 33.99 399 130 133 3 847.90 0.48 400 130 134 4 833.52 2.29 401 130 167 37 733.80 33.99 402 130 168 38 733.79 33.99 403 131 134 3 845.90 0.53 404 131 135 4 833.52 2.29 405 131 168 37 733.81 33.99 406 131 174 43 636.77 30.00 407 132 135 3 862.95 6.52 408 132 136 4 834.48 2.79 409 133 136 3 848.10 0.91 410 133 137 4 834.43 2.81 411 133 138 5 739.59 32.06 412 133 139 6 734.84 33.95 413 133 142 9 734.85 33.95 414 134 137 3 842.39 4.99 415 134 139 5 734.84 33.95 416 134 164 30 734.02 33.95 417 134 168 34 733.83 33.99 418 134 180 46 444.89 29.22 419 134 184 50 444.32 29.38 420 138 141 3 848.89 2.21 421 138 142 4 791.97 29.50 422 139 142 3 860.97 3.83 423 139 143 4 837.69 6.64 424 140 143 3 843.69 3.65 425 140 144 4 843.66 3.68 426 140 187 47 444.04 29.46 427 141 144 3 869.30 1.95 428 141 145 4 848.97 1.03 429 142 145 3 862.30 5.63 430 142 146 4 853.41 1.18 431 143 146 3 868.52 2.52 432 143 147 4 859.65 1.92 433 143 164 21 733.91 33.98 434 143 184 41 444.06 29.45 435 143 188 45 444.02 29.46 436 144 147 3 873.11 0.22 437 144 148 4 853.48 1.20 438 144 191 47 443.81 29.46 439 145 148 3 877.94 2.20 440 145 149 4 853.49 1.21 441 146 149 3 867.04 4.32 442 146 150 4 865.97 3.79 443 147 150 3 881.83 0.26 444 147 151 4 845.51 0.66 445 147 188 41 443.97 29.46 446 147 191 44 443.44 29.30 447 148 151 3 858.53 2.89 448 148 195 47 442.49 29.14 449 150 153 3 878.61 2.48 450 150 154 4 872.29 5.65 451 150 162 12 733.99 33.95 452 151 154 3 880.92 1.33 453 151 192 41 443.14 29.21 454 151 195 44 442.42 29.16 455 151 196 45 442.35 29.15 456 154 157 3 886.96 1.47 457 154 160 6 734.20 34.01 458 154 162 8 734.20 34.01 459 154 204 50 440.20 28.43 460 156 159 3 860.63 1.95 461 156 160 4 846.72 1.02 462 157 192 35 442.70 29.00 463 157 196 39 442.19 29.20 464 157 200 43 440.53 28.42 465 157 203 46 440.31 28.46 466 157 204 47 440.18 28.44 467 158 161 3 928.64 1.03 468 158 203 45 440.30 28.46 469 158 205 47 440.19 28.42 470 160 204 44 440.02 28.35 471 161 205 44 439.73 28.24 472 161 207 46 439.69 28.23 473 162 188 26 443.25 29.19 474 162 192 30 442.81 29.05 475 162 204 42 439.84 28.20 476 162 205 43 439.74 28.23 477 162 206 44 439.64 28.21 478 162 207 45 439.63 28.21 479 163 207 44 439.55 28.17 480 163 209 46 439.22 28.20 481 163 230 67 167.30 24.73 482 164 184 20 444.01 29.47 483 164 185 21 444.01 29.46 484 164 188 24 443.95 29.45 485 164 207 43 439.56 28.16 486 164 208 44 439.33 28.14 487 164 209 45 439.14 28.13 488 165 170 5 760.14 32.50 489 165 209 44 438.85 28.05 490 166 169 3 851.28 3.84 491 166 170 4 842.10 5.65 492 166 176 10 444.81 29.23 493 166 208 42 439.39 28.09 494 166 209 43 439.23 28.06 495 167 170 3 867.99 3.31 496 168 172 4 841.53 5.38 497 168 174 6 482.97 10.46 498 168 176 8 445.19 29.27 499 169 172 3 871.23 0.30 500 169 174 5 445.34 29.20 501 169 175 6 445.14 29.25 502 169 208 39 439.59 28.16 503 169 210 41 438.76 28.02 504 169 211 42 438.80 28.00 505 170 210 40 438.88 27.96 506 171 211 40 438.93 27.98 507 172 211 39 438.94 27.96 508 173 211 38 438.94 27.96 509 174 211 37 439.00 27.94 510 175 208 33 439.68 28.19 511 175 210 35 439.03 27.93 512 175 211 36 439.01 27.93 513 175 216 41 400.42 27.73 514 175 220 45 285.42 20.66 515 176 180 4 828.63 1.16 516 176 181 5 759.76 23.84 517 176 208 32 439.64 28.16 518 176 220 44 243.16 0.45 519 177 180 3 848.51 1.72 520 177 181 4 842.21 4.87 521 178 181 3 850.70 1.15 522 178 182 4 842.72 5.12 523 178 219 41 242.63 0.22 524 178 220 42 242.22 0.01 525 178 223 45 222.59 2.39 526 179 182 3 860.60 3.80 527 179 183 4 842.14 5.42 528 180 183 3 854.90 2.81 529 180 184 4 849.31 1.83 530 181 184 3 867.33 3.39 531 181 185 4 837.37 2.23 532 181 208 27 439.67 28.14 533 181 220 39 242.12 0.07 534 181 226 45 170.03 25.07 535 182 185 3 851.36 0.10 536 182 186 4 837.60 2.11 537 182 223 41 177.60 21.75 538 182 224 42 177.59 21.75 539 182 225 43 170.15 25.07 540 182 226 44 170.03 25.06 541 183 186 3 851.36 0.10 542 183 187 4 842.18 2.79 543 184 187 3 851.75 1.25 544 184 188 4 847.92 0.07 545 185 188 3 847.93 0.07 546 185 189 4 843.25 2.42 547 185 206 21 439.86 28.16 548 185 207 22 439.85 28.15 549 185 208 23 439.76 28.16 550 185 225 40 169.84 25.04 551 185 226 41 169.78 25.03 552 186 189 3 873.76 0.04 553 186 190 4 844.84 1.66 554 186 225 39 169.98 25.04 555 187 190 3 849.90 0.85 556 187 191 4 849.96 0.83 557 188 191 3 850.91 0.38 558 188 192 4 849.97 0.83 559 188 206 18 439.85 28.16 560 189 192 3 850.91 0.38 561 189 193 4 849.97 0.84 562 189 201 12 440.55 28.30 563 189 204 15 440.39 28.29 564 189 206 17 439.87 28.16 565 189 225 36 169.77 25.03 566 189 227 38 169.75 25.03 567 190 193 3 855.72 2.03 568 190 194 4 850.11 0.77 569 191 194 3 850.21 0.80 570 191 195 4 850.19 0.80 571 192 195 3 851.84 1.61 572 192 196 4 844.21 0.47 573 192 197 5 749.63 29.36 574 192 201 9 440.76 28.27 575 192 204 12 440.43 28.28 576 193 196 3 852.18 1.45 577 193 197 4 624.89 33.02 578 193 198 5 597.28 46.68 579 193 201 8 444.45 29.61 580 196 200 4 838.84 4.86 581 197 200 3 849.90 0.18 582 197 201 4 843.65 2.94 583 198 201 3 851.06 0.77 584 198 202 4 847.21 1.16 585 199 202 3 855.72 1.56 586 199 203 4 847.22 1.16 587 200 203 3 867.90 0.66 588 200 204 4 847.22 1.16 589 201 204 3 861.31 2.64 590 204 227 23 169.18 24.98 591 205 227 22 169.00 25.01 592 206 225 19 169.56 25.03 593 206 226 20 169.34 24.96 594 206 227 21 169.12 24.96 595 207 227 20 168.89 24.96 596 207 228 21 168.39 24.84 597 207 229 22 168.12 24.86 598 207 230 23 168.12 24.86 599 208 212 4 854.91 0.82 600 208 220 12 179.04 22.59 601 208 226 18 169.42 24.94 602 208 228 20 168.81 24.91 603 208 229 21 168.19 24.82 604 208 230 22 168.24 24.80 605 209 230 21 167.97 24.84 606 211 216 5 844.25 7.50 607 212 216 4 844.25 7.50 608 212 217 5 499.99 24.44 609 212 220 8 474.71 16.96 610 212 229 17 168.53 24.79 611 212 231 19 168.29 24.74 612 212 234 22 167.46 24.66 613 212 243 31 51.36 0.17 614 213 216 3 871.49 0.42 615 213 217 4 825.55 1.84 616 213 234 21 167.66 24.62 617 213 243 30 51.83 0.21 618 214 217 3 879.61 3.28 619 214 239 25 112.28 29.62 620 214 242 28 102.64 24.93 621 214 243 29 51.67 0.13 622 216 219 3 875.58 5.85 623 216 220 4 842.97 2.75 624 217 220 3 853.60 0.69 625 217 221 4 846.38 1.04 626 217 229 12 169.85 24.95 627 217 243 26 45.96 0.18 628 217 246 29 36.17 0.99 629 218 221 3 862.07 4.17 630 218 222 4 845.09 0.41 631 218 246 28 35.23 0.52 632 219 222 3 856.99 1.01 633 219 223 4 848.84 0.12 634 220 223 3 874.51 0.98 635 220 226 6 170.53 25.07 636 220 229 9 170.22 25.05 637 221 226 5 285.85 20.82 638 221 227 6 170.52 25.05 639 221 228 7 170.31 25.10 640 221 229 8 170.36 25.12 641 221 246 25 35.20 0.52 642 221 247 26 28.25 0.15 643 223 226 3 858.88 0.24 644 229 243 14 45.56 0.35 645 229 246 17 35.22 0.53 646 231 234 3 890.55 1.51 647 231 235 4 836.12 0.33 648 231 240 9 54.09 0.89 649 231 243 12 49.81 0.28 650 232 235 3 862.27 2.06 651 233 236 3 862.25 2.08 652 233 237 4 858.88 0.39 653 234 237 3 857.50 0.31 654 234 240 6 141.89 9.40 655 234 243 9 135.58 6.25 656 235 240 5 186.07 16.69 657 237 240 3 872.92 1.09 658 238 241 3 897.16 3.46 659 239 242 3 868.24 1.26 660 239 243 4 864.35 3.20 661 240 243 3 863.56 2.81 662 240 244 4 849.91 0.18 663 241 244 3 858.12 5.52 664 241 245 4 836.74 0.27 665 242 245 3 845.59 2.33 666 242 246 4 831.80 2.21 667 243 246 3 859.63 1.51 668 244 247 3 859.90 1.38 669 244 248 4 836.74 6.54 670 245 248 3 854.20 2.19