BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 203 200 145.65 15.67 2 3 204 201 144.25 14.98 3 3 224 221 115.12 0.50 4 3 227 224 114.63 0.45 5 4 34 30 811.82 0.84 6 4 35 31 811.82 0.83 7 4 36 32 811.78 0.83 8 4 37 33 811.77 0.83 9 4 38 34 811.77 0.83 10 4 205 201 144.25 14.97 11 5 37 32 811.77 0.83 12 5 38 33 811.77 0.83 13 5 221 216 115.22 0.48 14 5 227 222 115.20 0.49 15 5 247 242 21.57 0.55 16 6 38 32 811.78 0.83 17 6 40 34 811.77 0.83 18 6 90 84 238.60 58.93 19 6 124 118 215.69 50.29 20 6 161 155 215.50 50.24 21 6 205 199 148.50 16.77 22 6 207 201 144.22 14.96 23 6 227 221 115.34 0.53 24 6 228 222 115.20 0.48 25 6 229 223 115.20 0.48 26 7 37 30 811.84 0.83 27 7 38 31 811.81 0.83 28 7 39 32 811.78 0.83 29 7 40 33 811.78 0.83 30 7 229 222 115.23 0.48 31 7 240 233 56.88 0.42 32 7 243 236 47.11 0.16 33 7 244 237 37.97 0.07 34 7 247 240 25.12 0.29 35 8 40 32 811.78 0.83 36 8 42 34 811.78 0.83 37 8 207 199 148.80 16.89 38 8 229 221 115.33 0.52 39 8 230 222 115.16 0.48 40 8 231 223 115.14 0.48 41 8 240 232 57.16 0.55 42 9 40 31 811.82 0.83 43 9 41 32 811.78 0.83 44 9 42 33 811.78 0.83 45 9 231 222 115.19 0.48 46 9 235 226 85.51 0.03 47 9 240 231 57.18 0.56 48 10 42 32 811.80 0.83 49 10 64 54 461.56 0.36 50 10 92 82 241.13 60.13 51 10 95 85 240.32 59.88 52 10 230 220 116.06 0.55 53 10 231 221 115.27 0.49 54 10 232 222 115.14 0.48 55 10 235 225 95.45 0.77 56 11 14 3 871.09 1.54 57 11 20 9 812.88 0.64 58 11 23 12 812.62 0.77 59 11 24 13 812.87 0.64 60 11 27 16 812.52 0.71 61 11 41 30 811.84 0.83 62 11 42 31 811.81 0.83 63 11 43 32 811.79 0.83 64 11 51 40 700.12 5.12 65 11 64 53 461.59 0.36 66 11 235 224 107.58 1.47 67 11 236 225 88.38 0.25 68 12 44 32 811.80 0.83 69 12 64 52 461.63 0.41 70 12 95 83 241.12 60.19 71 12 97 85 241.06 60.24 72 12 232 220 116.06 0.55 73 13 44 31 811.81 0.83 74 14 44 30 811.84 0.83 75 14 236 222 114.60 0.38 76 15 19 4 833.48 0.80 77 15 47 32 811.50 0.94 78 16 19 3 845.00 6.42 79 16 20 4 833.95 1.03 80 17 20 3 866.10 4.33 81 17 21 4 833.95 1.03 82 17 47 30 810.34 0.32 83 17 50 33 760.50 0.36 84 18 21 3 855.00 1.22 85 18 22 4 852.88 0.65 86 19 22 3 858.41 1.46 87 19 23 4 852.89 0.65 88 20 23 3 864.96 3.15 89 20 24 4 854.99 0.40 90 20 47 27 810.15 0.28 91 20 50 30 722.99 3.42 92 20 51 31 709.89 0.05 93 20 54 34 634.08 33.52 94 21 24 3 868.46 1.41 95 21 25 4 863.23 2.35 96 21 54 33 634.07 33.52 97 22 25 3 869.62 0.83 98 22 26 4 863.26 2.33 99 23 26 3 866.79 0.57 100 23 27 4 856.37 1.11 101 23 235 212 112.48 0.35 102 23 236 213 91.05 0.48 103 23 237 214 87.26 0.26 104 23 238 215 84.03 0.73 105 24 27 3 856.37 1.11 106 24 28 4 856.36 1.11 107 24 51 27 696.06 3.13 108 24 54 30 467.11 0.61 109 24 55 31 466.01 0.72 110 25 28 3 866.99 4.02 111 25 29 4 856.36 1.11 112 26 29 3 866.99 4.02 113 26 238 212 77.59 0.30 114 26 241 215 61.95 1.82 115 27 30 3 873.49 0.77 116 27 41 14 811.82 0.83 117 27 55 28 466.00 0.71 118 27 59 32 462.78 0.42 119 27 235 208 95.71 0.73 120 27 237 210 85.45 0.10 121 27 240 213 63.68 0.93 122 27 241 214 56.61 0.40 123 28 54 26 466.10 0.72 124 28 55 27 465.30 0.37 125 28 56 28 465.10 0.31 126 28 58 30 464.35 0.58 127 28 59 31 463.25 0.20 128 30 58 28 462.82 0.18 129 30 241 211 56.68 0.36 130 30 244 214 49.61 0.53 131 30 245 215 49.13 0.35 132 30 248 218 45.29 0.47 133 31 248 217 39.17 1.05 134 32 37 5 813.65 0.56 135 32 39 7 812.60 0.60 136 32 58 26 462.24 0.31 137 32 59 27 462.19 0.33 138 32 248 216 31.20 1.88 139 33 36 3 866.68 2.66 140 33 37 4 823.59 3.85 141 34 37 3 831.64 3.36 142 34 58 24 461.84 0.35 143 37 244 207 43.68 1.32 144 37 247 210 25.64 0.89 145 37 248 211 20.03 0.12 146 38 205 167 148.62 16.80 147 39 59 20 461.99 0.29 148 39 60 21 461.85 0.35 149 39 244 205 38.93 0.63 150 40 60 20 461.86 0.35 151 40 61 21 461.85 0.35 152 40 62 22 461.84 0.35 153 40 90 50 240.87 60.02 154 41 51 10 466.41 0.92 155 41 55 14 463.42 0.34 156 41 59 18 462.13 0.30 157 41 60 19 462.08 0.30 158 41 61 20 461.85 0.35 159 41 62 21 461.84 0.35 160 42 62 20 461.84 0.35 161 42 63 21 461.76 0.35 162 42 64 22 461.68 0.30 163 42 90 48 241.31 60.15 164 42 91 49 241.22 60.15 165 42 92 50 241.16 60.16 166 42 124 82 241.13 60.13 167 43 47 4 813.88 0.88 168 43 48 5 758.94 28.24 169 43 51 8 758.82 28.30 170 43 62 19 462.06 0.30 171 43 64 21 461.77 0.35 172 44 47 3 845.63 2.89 173 44 48 4 838.34 6.53 174 44 64 20 461.77 0.35 175 45 48 3 854.63 1.57 176 45 63 18 462.04 0.35 177 47 50 3 866.34 2.02 178 47 51 4 851.92 0.24 179 48 51 3 860.49 4.52 180 48 52 4 857.26 2.91 181 48 61 13 462.35 0.29 182 48 62 14 462.32 0.30 183 48 63 15 462.26 0.29 184 48 88 40 241.81 60.07 185 49 52 3 863.37 5.96 186 49 53 4 852.48 0.52 187 49 86 37 241.62 60.20 188 50 53 3 867.74 3.78 189 50 54 4 854.79 1.68 190 51 54 3 859.16 0.51 191 51 55 4 845.85 3.85 192 52 55 3 878.11 3.21 193 52 56 4 871.78 0.20 194 52 57 5 845.81 3.86 195 52 59 7 692.68 59.21 196 52 60 8 463.40 0.23 197 52 61 9 462.53 0.35 198 55 59 4 819.37 3.16 199 55 61 6 583.83 60.48 200 56 59 3 862.27 1.72 201 59 244 185 44.52 0.60 202 60 89 29 241.24 60.18 203 60 90 30 241.08 60.20 204 61 89 28 241.25 60.19 205 61 90 29 241.16 60.16 206 62 88 26 241.33 60.19 207 62 90 28 241.16 60.16 208 63 66 3 937.15 0.72 209 63 78 15 243.48 59.60 210 63 83 20 242.27 60.04 211 63 88 25 241.97 60.02 212 63 90 27 241.27 60.19 213 63 91 28 241.24 60.18 214 63 92 29 241.24 60.18 215 64 92 28 241.24 60.18 216 65 92 27 241.47 60.09 217 65 98 33 241.62 60.01 218 65 112 47 242.89 59.57 219 66 78 12 243.55 59.63 220 66 79 13 243.46 59.64 221 66 80 14 243.40 59.65 222 66 91 25 242.51 59.79 223 66 93 27 241.84 60.09 224 66 112 46 242.89 59.57 225 66 116 50 243.02 59.64 226 67 77 10 244.13 59.75 227 67 78 11 243.77 59.57 228 67 79 12 243.48 59.66 229 67 112 45 243.04 59.61 230 68 108 40 243.03 59.59 231 68 111 43 243.06 59.60 232 68 112 44 243.05 59.61 233 69 74 5 475.98 46.95 234 69 79 10 244.18 59.73 235 70 74 4 890.63 0.85 236 70 79 9 244.18 59.73 237 71 79 8 244.27 59.69 238 71 82 11 244.26 59.70 239 74 77 3 866.23 0.83 240 78 82 4 845.53 0.61 241 79 82 3 853.90 1.68 242 79 83 4 845.55 0.62 243 80 83 3 859.78 3.20 244 80 84 4 845.84 0.48 245 80 115 35 351.37 8.17 246 80 116 36 296.44 35.63 247 80 119 39 352.01 8.02 248 80 121 41 351.51 8.24 249 81 84 3 860.76 3.68 250 81 119 38 378.63 5.19 251 82 85 3 860.75 3.69 252 82 86 4 846.22 0.67 253 83 86 3 864.79 1.67 254 83 87 4 844.28 1.63 255 83 88 5 432.76 20.00 256 83 121 38 473.12 0.04 257 84 87 3 839.61 6.87 258 84 119 35 479.22 3.00 259 84 120 36 479.22 3.00 260 84 121 37 473.12 0.04 261 88 121 33 539.69 2.60 262 89 121 32 539.69 2.60 263 89 122 33 539.67 2.60 264 90 122 32 539.69 2.59 265 90 124 34 539.63 2.60 266 90 161 71 539.66 2.59 267 91 116 25 539.94 2.58 268 91 121 30 539.91 2.58 269 91 122 31 539.84 2.58 270 91 123 32 539.69 2.59 271 91 124 33 539.67 2.59 272 92 95 3 909.50 1.76 273 92 124 32 539.69 2.59 274 92 126 34 539.68 2.59 275 92 230 138 165.36 22.75 276 93 109 16 539.99 2.54 277 93 112 19 540.02 2.54 278 93 113 20 540.00 2.54 279 93 116 23 540.03 2.54 280 93 123 30 539.90 2.60 281 93 124 31 539.82 2.57 282 93 125 32 539.71 2.59 283 93 126 33 539.69 2.58 284 94 99 5 764.38 30.21 285 94 104 10 540.03 2.54 286 94 109 15 540.00 2.53 287 94 126 32 539.70 2.60 288 95 98 3 852.01 3.77 289 95 99 4 835.43 0.17 290 95 126 31 539.75 2.59 291 95 165 70 539.68 2.60 292 95 230 135 166.22 22.34 293 96 99 3 835.43 0.17 294 96 100 4 835.42 0.17 295 96 165 69 539.69 2.60 296 96 167 71 539.17 2.68 297 96 170 74 496.34 2.35 298 97 100 3 843.88 4.41 299 97 101 4 832.89 1.43 300 97 102 5 764.72 30.04 301 98 102 4 764.26 30.19 302 98 103 5 764.26 30.19 303 98 104 6 540.22 2.59 304 99 104 5 540.08 2.53 305 99 106 7 540.22 2.60 306 99 109 10 540.21 2.60 307 99 126 27 540.01 2.56 308 99 127 28 540.00 2.56 309 99 146 47 540.01 2.55 310 104 108 4 849.83 0.62 311 104 109 5 849.83 0.62 312 104 112 8 767.12 30.25 313 105 108 3 864.42 2.51 314 105 109 4 859.07 0.18 315 106 109 3 865.35 2.96 316 106 110 4 859.07 0.18 317 106 146 40 540.11 2.62 318 106 149 43 540.25 2.55 319 107 110 3 859.07 0.18 320 107 111 4 859.07 0.18 321 108 111 3 871.38 6.34 322 108 112 4 859.05 0.19 323 109 112 3 876.90 3.58 324 109 113 4 854.82 1.88 325 109 125 16 540.12 2.66 326 109 150 41 540.53 2.56 327 110 113 3 861.16 0.63 328 110 114 4 851.01 0.98 329 110 149 39 540.50 2.58 330 110 150 40 540.53 2.56 331 110 152 42 542.53 1.71 332 110 153 43 541.03 2.45 333 111 114 3 863.11 3.60 334 111 115 4 851.02 0.97 335 112 115 3 859.41 0.93 336 112 116 4 851.69 0.24 337 113 116 3 854.80 1.79 338 113 117 4 851.69 0.24 339 113 123 10 540.60 2.54 340 113 125 12 540.48 2.58 341 113 150 37 540.62 2.54 342 113 153 40 560.58 1.50 343 113 154 41 571.51 3.98 344 113 160 47 540.91 2.41 345 114 117 3 876.38 3.08 346 114 118 4 848.60 1.31 347 114 153 39 551.07 0.30 348 115 118 3 877.16 2.69 349 115 119 4 848.60 1.30 350 116 119 3 875.77 2.00 351 116 120 4 848.58 1.30 352 116 121 5 743.88 36.58 353 117 120 3 848.31 2.64 354 117 121 4 826.25 1.28 355 117 153 36 597.36 1.12 356 117 154 37 594.04 0.54 357 117 155 38 617.96 2.35 358 117 160 43 589.58 2.69 359 122 159 37 670.98 1.80 360 123 159 36 671.08 1.81 361 123 160 37 671.01 1.81 362 123 161 38 671.01 1.81 363 124 161 37 671.02 1.81 364 124 163 39 671.00 1.81 365 125 150 25 671.41 1.79 366 125 160 35 671.20 1.80 367 125 161 36 671.09 1.81 368 125 162 37 671.03 1.81 369 125 163 38 671.02 1.81 370 126 163 37 671.06 1.80 371 126 165 39 671.00 1.81 372 126 230 104 166.12 22.38 373 127 143 16 671.58 1.76 374 127 146 19 671.56 1.77 375 127 147 20 671.57 1.77 376 127 150 23 671.56 1.78 377 127 162 35 671.21 1.80 378 127 163 36 671.09 1.81 379 127 164 37 671.04 1.81 380 127 165 38 671.01 1.81 381 127 188 61 267.56 19.05 382 128 143 15 671.58 1.75 383 128 146 18 671.62 1.78 384 128 164 36 671.08 1.81 385 128 165 37 671.03 1.81 386 128 167 39 670.96 1.83 387 129 133 4 845.78 1.83 388 129 134 5 835.33 3.39 389 129 139 10 671.78 1.79 390 129 142 13 671.72 1.80 391 129 143 14 671.71 1.79 392 129 164 35 671.18 1.81 393 129 167 38 670.99 1.82 394 129 168 39 670.97 1.81 395 130 133 3 862.73 0.28 396 130 134 4 847.48 1.22 397 130 167 37 670.99 1.82 398 130 168 38 670.95 1.82 399 131 134 3 864.22 0.85 400 131 135 4 859.40 1.54 401 131 168 37 670.95 1.82 402 131 172 41 622.09 1.29 403 131 174 43 523.59 15.97 404 132 135 3 865.35 0.29 405 132 136 4 859.52 1.48 406 133 136 3 860.56 0.97 407 133 137 4 850.48 3.04 408 133 138 5 690.34 0.12 409 133 142 9 671.85 1.78 410 134 137 3 842.57 0.17 411 134 139 5 682.77 3.66 412 134 164 30 671.28 1.79 413 134 168 34 671.00 1.84 414 134 184 50 268.01 19.08 415 138 141 3 882.82 6.22 416 138 142 4 841.30 1.91 417 139 142 3 876.52 3.08 418 139 143 4 853.18 2.59 419 139 184 45 267.87 19.00 420 140 143 3 876.52 3.08 421 140 144 4 850.37 4.00 422 141 144 3 875.99 3.33 423 141 145 4 852.20 3.09 424 142 145 3 863.18 2.42 425 142 146 4 843.12 1.46 426 143 146 3 854.82 5.46 427 143 147 4 843.12 1.45 428 143 164 21 671.22 1.80 429 143 184 41 267.77 19.02 430 143 188 45 267.61 19.04 431 143 191 48 267.56 19.04 432 144 147 3 862.03 1.85 433 144 148 4 838.44 1.60 434 144 191 47 267.54 19.03 435 145 149 4 854.14 5.12 436 146 149 3 874.70 1.79 437 146 150 4 869.40 0.85 438 147 150 3 877.01 2.80 439 147 151 4 838.85 1.84 440 147 188 41 267.54 19.03 441 147 191 44 267.51 19.03 442 148 151 3 867.18 4.66 443 148 191 43 267.52 19.03 444 148 195 47 266.63 19.11 445 150 153 3 872.81 0.70 446 150 162 12 671.20 1.79 447 151 154 3 872.81 0.70 448 151 192 41 267.01 19.17 449 151 195 44 262.83 20.98 450 151 196 45 261.92 20.55 451 154 157 3 896.24 2.87 452 154 160 6 671.25 1.80 453 154 204 50 248.44 24.93 454 156 159 3 863.93 2.77 455 156 160 4 840.07 1.54 456 157 192 35 266.78 19.07 457 157 196 39 259.73 19.96 458 157 200 43 248.69 24.90 459 157 203 46 248.44 25.01 460 157 204 47 248.34 24.98 461 158 161 3 946.63 0.98 462 158 203 45 248.44 25.01 463 158 205 47 248.34 24.98 464 160 204 44 248.33 24.98 465 161 205 44 247.89 25.11 466 161 207 46 243.08 24.75 467 162 188 26 267.09 19.21 468 162 192 30 266.99 19.16 469 162 204 42 248.34 24.98 470 162 205 43 248.11 25.01 471 162 206 44 243.08 24.75 472 162 207 45 243.08 24.75 473 163 207 44 242.97 24.71 474 163 209 46 242.31 24.57 475 163 230 67 166.12 22.38 476 164 184 20 267.56 19.05 477 164 185 21 267.52 19.05 478 164 188 24 267.51 19.05 479 164 207 43 243.08 24.75 480 164 208 44 242.88 24.75 481 164 209 45 242.78 24.71 482 165 209 44 241.83 25.01 483 166 169 3 870.27 0.18 484 166 170 4 844.25 0.33 485 166 176 10 268.10 19.10 486 166 208 42 244.61 25.54 487 166 209 43 242.88 24.75 488 166 210 44 241.84 25.01 489 167 170 3 840.64 0.60 490 168 172 4 836.64 2.94 491 168 174 6 398.60 3.71 492 168 176 8 278.04 21.43 493 168 180 12 268.15 19.12 494 169 172 3 856.26 2.92 495 169 174 5 321.00 42.52 496 169 175 6 277.31 21.08 497 169 176 7 271.59 18.22 498 176 180 4 784.69 28.95 499 176 208 32 246.80 25.28 500 177 180 3 854.70 5.66 501 177 181 4 854.70 5.66 502 178 181 3 857.08 4.46 503 178 182 4 781.50 27.35 504 178 219 41 179.14 25.62 505 178 220 42 179.16 25.61 506 178 223 45 173.21 23.32 507 178 226 48 171.50 23.73 508 179 182 3 781.84 27.53 509 179 183 4 781.86 27.54 510 179 223 44 173.19 23.32 511 180 184 4 843.90 3.20 512 181 184 3 852.42 1.06 513 181 185 4 777.02 30.09 514 181 208 27 247.10 25.42 515 181 226 45 171.05 23.74 516 182 185 3 849.17 0.57 517 182 186 4 832.44 5.44 518 182 223 41 171.23 23.86 519 182 224 42 171.52 23.73 520 182 225 43 171.22 23.79 521 182 226 44 171.06 23.73 522 183 186 3 850.64 5.00 523 183 187 4 853.23 6.25 524 184 187 3 860.21 2.77 525 184 188 4 842.82 1.05 526 185 188 3 863.80 0.96 527 185 189 4 859.83 2.58 528 185 206 21 248.34 24.98 529 185 207 22 248.31 24.98 530 185 208 23 248.25 24.95 531 185 225 40 170.88 23.71 532 185 226 41 170.83 23.73 533 186 189 3 863.79 0.96 534 186 190 4 849.66 2.36 535 186 225 39 170.93 23.71 536 187 190 3 864.26 4.94 537 188 191 3 864.26 4.94 538 188 192 4 852.31 1.03 539 188 206 18 248.34 24.98 540 189 192 3 850.42 2.62 541 189 193 4 833.31 5.95 542 189 201 12 248.89 24.85 543 189 204 15 248.71 24.93 544 189 206 17 248.40 24.96 545 189 225 36 170.83 23.75 546 189 227 38 170.80 23.75 547 190 193 3 845.44 0.12 548 190 194 4 845.59 0.20 549 191 194 3 860.63 3.42 550 191 195 4 826.59 2.60 551 192 195 3 857.37 4.75 552 192 196 4 724.49 1.36 553 192 197 5 697.12 12.32 554 192 201 9 248.77 24.91 555 192 204 12 248.69 24.92 556 193 197 4 382.94 0.94 557 193 198 5 294.92 16.63 558 193 201 8 248.91 24.86 559 196 200 4 829.99 1.66 560 197 200 3 854.57 3.53 561 197 201 4 837.09 5.21 562 198 201 3 859.35 1.14 563 198 202 4 844.19 1.66 564 199 202 3 856.43 0.33 565 199 203 4 844.18 1.66 566 200 203 3 852.88 1.45 567 200 204 4 839.74 3.87 568 201 204 3 861.18 2.75 569 204 227 23 170.34 23.61 570 205 227 22 170.34 23.61 571 206 225 19 170.50 23.61 572 206 226 20 170.38 23.62 573 206 227 21 170.34 23.61 574 207 227 20 170.09 23.48 575 207 228 21 167.39 22.16 576 207 229 22 167.28 22.11 577 207 230 23 167.16 22.07 578 208 212 4 880.68 0.07 579 208 220 12 170.56 23.70 580 208 226 18 170.47 23.62 581 208 228 20 169.91 23.38 582 208 229 21 167.34 22.14 583 208 230 22 167.34 22.14 584 209 230 21 166.69 22.30 585 211 216 5 838.33 1.51 586 212 216 4 838.33 1.51 587 212 217 5 721.94 55.72 588 212 220 8 375.90 4.90 589 212 229 17 169.11 22.96 590 212 231 19 167.40 22.12 591 212 234 22 166.42 22.22 592 212 243 31 106.47 25.96 593 213 216 3 860.47 0.07 594 213 217 4 840.31 0.85 595 213 234 21 166.85 22.02 596 213 243 30 107.19 25.62 597 214 217 3 877.11 0.06 598 214 239 25 123.14 32.48 599 214 242 28 111.14 27.55 600 214 243 29 107.19 25.62 601 216 219 3 848.76 2.17 602 216 220 4 836.96 0.88 603 217 220 3 845.98 3.56 604 217 221 4 839.53 0.48 605 217 229 12 170.93 23.72 606 217 243 26 103.87 27.01 607 217 246 29 89.03 25.55 608 218 221 3 850.68 1.21 609 218 222 4 839.58 0.46 610 218 246 28 88.34 25.38 611 219 222 3 864.41 1.30 612 219 223 4 843.58 0.36 613 220 223 3 872.14 5.67 614 220 226 6 694.59 1.96 615 220 229 9 171.25 23.78 616 221 226 5 694.59 1.95 617 221 227 6 477.28 110.61 618 221 228 7 237.45 9.30 619 221 229 8 171.31 23.77 620 221 246 25 37.62 0.03 621 221 247 26 33.12 0.29 622 223 226 3 834.82 7.06 623 229 243 14 102.79 26.71 624 229 246 17 37.91 0.17 625 231 234 3 890.12 1.88 626 231 235 4 830.25 2.14 627 231 240 9 107.25 25.59 628 231 243 12 105.38 25.46 629 232 235 3 883.00 0.61 630 233 236 3 885.37 1.80 631 233 237 4 872.15 4.81 632 234 237 3 863.78 0.14 633 234 240 6 242.18 55.06 634 234 243 9 238.33 56.98 635 235 240 5 314.88 18.71 636 237 240 3 884.70 0.67 637 238 241 3 892.97 1.17 638 239 242 3 873.22 5.08 639 239 243 4 861.77 0.65 640 240 243 3 861.77 0.65 641 240 244 4 855.75 3.66 642 241 244 3 861.77 0.65 643 241 245 4 855.74 3.66 644 242 245 3 861.76 0.64 645 242 246 4 855.73 3.66 646 243 246 3 864.83 0.93 647 244 247 3 875.06 2.60 648 244 248 4 865.83 1.50 649 245 248 3 838.53 4.17