BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 203 200 172.28 1.56 2 3 204 201 170.78 0.83 3 3 205 202 169.69 1.33 4 3 224 221 117.38 0.92 5 3 225 222 117.34 0.90 6 3 227 224 117.03 0.82 7 4 34 30 672.50 0.04 8 4 35 31 672.49 0.04 9 4 36 32 672.47 0.04 10 4 37 33 672.46 0.03 11 4 38 34 672.46 0.03 12 4 205 201 171.35 1.11 13 5 37 32 672.48 0.03 14 5 38 33 672.47 0.03 15 5 221 216 117.33 0.90 16 5 226 221 117.38 0.92 17 5 227 222 117.33 0.91 18 5 247 242 21.15 0.57 19 6 37 31 672.50 0.04 20 6 38 32 672.48 0.03 21 6 40 34 672.47 0.03 22 6 90 84 271.97 41.19 23 6 124 118 243.21 32.42 24 6 161 155 243.03 32.51 25 6 205 199 174.00 1.55 26 6 207 201 171.35 1.11 27 6 227 221 117.86 0.70 28 6 228 222 117.30 0.93 29 6 229 223 117.29 0.93 30 7 37 30 672.57 0.03 31 7 38 31 672.51 0.03 32 7 39 32 672.47 0.03 33 7 40 33 672.47 0.03 34 7 229 222 117.31 0.93 35 7 240 233 51.94 0.01 36 7 243 236 45.79 0.01 37 7 244 237 36.91 0.72 38 7 247 240 22.95 0.07 39 8 40 32 672.47 0.03 40 8 42 34 672.47 0.03 41 8 229 221 118.14 0.57 42 8 230 222 117.28 0.93 43 8 231 223 117.26 0.92 44 8 240 232 51.97 0.03 45 9 40 31 672.51 0.03 46 9 41 32 672.48 0.03 47 9 42 33 672.47 0.02 48 9 231 222 117.30 0.93 49 9 235 226 83.31 0.29 50 9 240 231 56.47 2.28 51 10 42 32 672.49 0.03 52 10 64 54 384.81 40.41 53 10 92 82 274.71 41.54 54 10 95 85 272.30 42.86 55 10 230 220 119.25 0.39 56 10 231 221 117.50 0.86 57 10 232 222 117.28 0.93 58 10 235 225 85.84 0.79 59 11 14 3 873.08 1.49 60 11 20 9 673.99 0.20 61 11 23 12 673.91 0.20 62 11 24 13 673.88 0.18 63 11 27 16 672.97 0.22 64 11 41 30 672.56 0.03 65 11 42 31 672.51 0.02 66 11 43 32 672.49 0.03 67 11 51 40 574.89 1.65 68 11 64 53 384.89 40.44 69 11 235 224 109.26 1.53 70 11 236 225 85.66 0.87 71 12 44 32 672.50 0.03 72 12 64 52 384.87 40.45 73 12 95 83 274.75 41.75 74 12 97 85 274.94 41.65 75 12 232 220 119.25 0.38 76 13 44 31 672.52 0.03 77 14 44 30 672.54 0.01 78 14 236 222 116.85 0.96 79 15 19 4 851.45 5.10 80 15 20 5 832.81 2.77 81 15 47 32 672.26 0.09 82 16 19 3 853.50 4.44 83 16 20 4 853.51 4.45 84 17 20 3 862.97 0.25 85 17 21 4 853.52 4.45 86 17 47 30 671.44 0.54 87 17 50 33 620.31 0.04 88 18 21 3 866.80 1.67 89 18 22 4 853.57 4.45 90 19 22 3 874.12 2.00 91 19 23 4 853.56 4.44 92 20 23 3 864.11 2.92 93 20 24 4 853.28 4.32 94 20 47 27 671.39 0.55 95 20 50 30 589.77 5.76 96 20 51 31 588.39 6.02 97 20 54 34 562.13 2.25 98 21 24 3 864.11 2.92 99 21 25 4 860.09 0.92 100 21 50 29 594.78 3.22 101 21 54 33 562.12 2.25 102 22 25 3 860.19 0.95 103 22 26 4 860.11 0.91 104 22 238 216 83.66 0.22 105 23 26 3 865.44 3.60 106 23 27 4 859.75 0.78 107 23 235 212 112.38 2.85 108 23 236 213 86.39 0.52 109 23 237 214 83.59 0.20 110 23 238 215 83.23 0.34 111 24 27 3 852.88 4.82 112 24 28 4 851.93 4.69 113 24 51 27 571.56 2.52 114 24 54 30 561.78 2.29 115 24 55 31 450.25 8.54 116 25 28 3 856.96 2.77 117 25 29 4 852.26 4.53 118 26 29 3 859.72 4.16 119 27 30 3 875.58 5.38 120 27 41 14 672.64 0.03 121 27 55 28 513.38 23.00 122 27 59 32 386.57 39.93 123 27 235 208 94.73 3.66 124 27 237 210 83.56 0.21 125 27 240 213 59.09 1.49 126 27 241 214 51.65 0.18 127 28 54 26 513.43 22.98 128 28 55 27 450.26 8.54 129 28 56 28 450.10 8.47 130 28 58 30 448.24 9.36 131 28 59 31 386.81 40.04 132 30 58 28 386.50 40.21 133 30 241 211 51.50 0.21 134 30 244 214 50.31 0.12 135 30 245 215 47.38 0.70 136 30 248 218 42.89 0.87 137 31 248 217 38.29 0.12 138 32 39 7 673.13 0.06 139 32 58 26 385.36 40.40 140 32 59 27 385.39 40.38 141 32 248 216 30.41 0.23 142 33 36 3 866.26 2.56 143 33 37 4 840.73 6.01 144 34 37 3 850.57 1.10 145 34 58 24 385.00 40.47 146 37 244 207 38.76 0.12 147 37 247 210 27.66 1.42 148 37 248 211 21.14 0.56 149 38 205 167 174.56 1.70 150 39 59 20 385.13 40.43 151 39 60 21 385.13 40.43 152 39 244 205 37.41 0.47 153 40 60 20 385.13 40.43 154 40 61 21 385.10 40.44 155 40 62 22 385.09 40.44 156 40 90 50 274.72 41.38 157 41 51 10 516.91 24.74 158 41 55 14 386.68 40.18 159 41 59 18 385.43 40.45 160 41 60 19 385.29 40.46 161 41 61 20 385.13 40.43 162 41 62 21 385.12 40.43 163 42 62 20 385.10 40.44 164 42 63 21 385.07 40.45 165 42 64 22 384.87 40.45 166 42 90 48 275.30 41.29 167 42 91 49 275.30 41.27 168 42 92 50 275.32 41.27 169 42 124 82 275.00 41.24 170 42 230 188 119.29 0.36 171 43 47 4 758.02 39.03 172 43 48 5 577.83 0.74 173 43 51 8 561.76 2.33 174 43 62 19 385.26 40.47 175 43 64 21 385.06 40.46 176 44 47 3 845.64 4.82 177 44 48 4 818.15 10.29 178 44 64 20 385.05 40.47 179 45 48 3 847.72 5.69 180 47 50 3 868.53 4.72 181 47 51 4 838.19 1.75 182 48 51 3 861.03 0.98 183 48 52 4 844.99 1.66 184 48 61 13 385.61 40.40 185 48 62 14 385.52 40.41 186 48 63 15 385.49 40.42 187 48 88 40 275.32 41.31 188 49 52 3 864.50 1.50 189 49 53 4 852.25 3.58 190 50 53 3 861.04 0.98 191 50 54 4 856.64 1.39 192 51 54 3 871.77 1.50 193 51 55 4 850.88 4.27 194 52 55 3 872.98 0.90 195 52 56 4 868.16 3.30 196 52 57 5 845.87 2.02 197 52 59 7 684.54 55.75 198 52 60 8 386.85 40.06 199 52 61 9 385.84 40.41 200 55 59 4 819.13 8.51 201 55 61 6 626.29 26.62 202 56 59 3 889.95 2.62 203 59 244 185 42.24 1.06 204 60 89 29 274.91 41.38 205 60 90 30 274.67 41.48 206 61 89 28 275.10 41.29 207 61 90 29 274.68 41.48 208 62 88 26 275.23 41.34 209 62 90 28 274.67 41.48 210 63 78 15 278.89 40.77 211 63 83 20 276.04 41.59 212 63 88 25 275.44 41.32 213 63 90 27 275.31 41.29 214 63 91 28 275.31 41.29 215 63 92 29 275.33 41.27 216 64 92 28 275.33 41.27 217 65 92 27 275.39 41.27 218 65 98 33 276.64 40.83 219 65 112 47 277.76 40.52 220 66 78 12 279.06 40.81 221 66 79 13 279.00 40.79 222 66 80 14 278.92 40.79 223 66 91 25 277.06 40.65 224 66 93 27 277.02 40.62 225 66 112 46 277.95 40.57 226 66 116 50 278.18 40.52 227 67 77 10 279.44 40.79 228 67 78 11 279.18 40.83 229 67 79 12 279.01 40.79 230 67 112 45 278.35 40.44 231 68 108 40 278.38 40.41 232 68 111 43 278.56 40.34 233 68 112 44 278.39 40.42 234 69 74 5 826.86 5.31 235 69 79 10 280.31 40.49 236 70 74 4 894.64 8.36 237 70 79 9 280.31 40.46 238 71 74 3 955.27 0.34 239 71 79 8 280.42 40.48 240 71 81 10 280.42 40.48 241 71 82 11 280.42 40.48 242 74 77 3 884.94 4.31 243 78 82 4 843.11 6.38 244 79 82 3 865.88 5.01 245 79 83 4 848.90 3.48 246 80 83 3 857.77 0.96 247 80 84 4 848.91 3.48 248 80 115 35 298.64 32.72 249 80 116 36 298.41 32.60 250 80 119 39 405.00 16.28 251 80 121 41 403.26 17.11 252 81 84 3 873.43 2.55 253 81 85 4 848.93 3.48 254 81 119 38 405.01 16.27 255 82 85 3 862.12 3.11 256 82 86 4 853.03 1.42 257 83 86 3 859.49 1.80 258 83 87 4 845.52 2.33 259 83 88 5 579.85 50.06 260 83 121 38 479.34 3.58 261 84 87 3 829.40 3.29 262 84 119 35 489.86 1.57 263 84 120 36 489.86 1.57 264 84 121 37 483.19 1.76 265 88 121 33 541.02 1.26 266 89 121 32 541.03 1.26 267 89 122 33 541.01 1.26 268 90 122 32 541.01 1.26 269 90 124 34 540.98 1.25 270 90 161 71 541.00 1.26 271 91 116 25 541.33 1.24 272 91 121 30 541.24 1.21 273 91 122 31 541.20 1.20 274 91 123 32 541.02 1.25 275 91 124 33 541.01 1.26 276 92 95 3 915.60 2.02 277 92 124 32 541.02 1.25 278 92 126 34 540.99 1.27 279 92 230 138 119.97 0.38 280 93 109 16 541.23 1.20 281 93 112 19 541.38 1.23 282 93 113 20 541.34 1.25 283 93 116 23 541.47 1.21 284 93 123 30 541.24 1.21 285 93 124 31 541.10 1.25 286 93 125 32 541.02 1.26 287 93 126 33 541.00 1.26 288 94 99 5 755.62 30.26 289 94 109 15 541.33 1.23 290 94 126 32 541.02 1.25 291 95 98 3 855.47 5.46 292 95 99 4 830.63 7.21 293 95 126 31 541.07 1.24 294 95 165 70 540.98 1.27 295 95 230 135 121.13 0.18 296 96 99 3 841.34 1.85 297 96 100 4 819.58 1.68 298 96 165 69 541.01 1.27 299 96 167 71 540.09 1.56 300 96 170 74 501.50 0.43 301 97 100 3 860.37 2.79 302 97 101 4 819.57 1.68 303 98 102 4 621.54 36.42 304 98 103 5 621.42 36.48 305 98 104 6 544.16 0.45 306 99 103 4 544.21 0.46 307 99 104 5 544.16 0.45 308 99 106 7 544.17 0.45 309 99 109 10 542.62 1.20 310 99 126 27 541.50 1.26 311 99 127 28 541.49 1.26 312 99 146 47 541.53 1.26 313 100 103 3 867.05 0.78 314 104 108 4 827.47 7.77 315 104 112 8 804.45 2.64 316 105 108 3 847.54 3.54 317 105 109 4 841.74 0.64 318 106 109 3 849.71 3.75 319 106 110 4 848.43 3.10 320 106 146 40 541.59 1.28 321 106 149 43 541.61 1.28 322 107 110 3 848.43 3.10 323 107 111 4 848.43 3.10 324 108 111 3 859.76 3.43 325 108 112 4 848.39 3.12 326 109 112 3 863.30 5.19 327 109 113 4 844.92 0.67 328 109 125 16 541.87 1.20 329 109 150 41 542.44 1.36 330 110 113 3 859.97 6.86 331 110 114 4 844.91 0.67 332 110 149 39 542.06 1.21 333 110 150 40 542.00 1.24 334 110 152 42 543.44 1.50 335 110 153 43 543.47 1.48 336 111 114 3 852.66 0.97 337 111 115 4 847.14 1.78 338 112 115 3 851.32 0.30 339 112 116 4 843.59 0.54 340 113 116 3 858.66 3.98 341 113 117 4 847.68 1.50 342 113 123 10 542.71 1.45 343 113 125 12 542.10 1.19 344 113 150 37 543.40 1.55 345 113 153 40 543.83 1.57 346 113 154 41 550.99 5.16 347 113 160 47 543.58 1.66 348 114 117 3 858.31 0.12 349 114 118 4 852.38 0.85 350 114 153 39 543.96 1.68 351 115 118 3 852.39 0.85 352 115 119 4 852.38 0.85 353 116 119 3 869.06 4.13 354 116 120 4 849.14 2.48 355 116 121 5 807.70 7.00 356 116 123 7 543.84 1.68 357 117 120 3 850.58 0.53 358 117 121 4 822.45 1.87 359 117 153 36 690.83 4.79 360 117 154 37 691.35 4.55 361 117 160 43 560.44 4.09 362 122 159 37 784.51 1.55 363 123 159 36 784.62 1.54 364 123 160 37 784.57 1.54 365 123 161 38 784.57 1.54 366 124 161 37 784.57 1.54 367 124 163 39 784.56 1.54 368 124 207 83 283.88 43.27 369 125 150 25 784.82 1.56 370 125 160 35 784.74 1.55 371 125 161 36 784.68 1.55 372 125 162 37 784.59 1.54 373 125 163 38 784.58 1.55 374 126 163 37 784.59 1.54 375 126 165 39 784.55 1.54 376 126 230 104 120.76 0.14 377 127 143 16 785.22 1.49 378 127 146 19 785.15 1.49 379 127 147 20 785.15 1.49 380 127 150 23 785.13 1.49 381 127 162 35 784.74 1.55 382 127 163 36 784.68 1.55 383 127 164 37 784.63 1.56 384 127 165 38 784.61 1.56 385 127 188 61 309.42 33.67 386 128 143 15 785.22 1.49 387 128 146 18 785.20 1.49 388 128 164 36 784.65 1.56 389 128 165 37 784.62 1.56 390 128 167 39 784.51 1.54 391 129 133 4 828.37 6.38 392 129 134 5 818.51 1.45 393 129 139 10 785.27 1.50 394 129 142 13 785.25 1.51 395 129 143 14 785.25 1.50 396 129 164 35 784.74 1.55 397 129 167 38 784.57 1.56 398 129 168 39 784.53 1.55 399 130 133 3 839.32 1.09 400 130 134 4 832.28 4.43 401 130 167 37 784.57 1.57 402 130 168 38 784.55 1.56 403 131 134 3 858.15 2.69 404 131 135 4 832.33 4.43 405 131 168 37 784.56 1.56 406 131 172 41 735.86 3.27 407 131 174 43 709.91 3.74 408 132 135 3 858.08 2.72 409 132 136 4 832.33 4.44 410 133 136 3 858.08 2.72 411 133 137 4 838.24 1.49 412 133 138 5 785.58 1.40 413 133 142 9 785.30 1.49 414 134 137 3 843.45 4.59 415 134 139 5 785.37 1.51 416 134 164 30 784.84 1.54 417 134 168 34 784.61 1.57 418 134 184 50 310.94 33.20 419 138 141 3 846.94 2.77 420 138 142 4 844.55 1.57 421 139 142 3 850.56 0.96 422 139 143 4 844.57 1.58 423 139 184 45 310.44 33.29 424 140 143 3 868.05 5.69 425 140 144 4 844.59 1.59 426 140 187 47 310.20 33.33 427 141 144 3 850.56 0.96 428 141 145 4 848.24 0.20 429 142 145 3 853.40 2.77 430 142 146 4 848.23 0.20 431 143 146 3 848.23 0.20 432 143 147 4 846.34 0.74 433 143 164 21 784.72 1.53 434 143 184 41 310.28 33.29 435 143 188 45 309.54 33.61 436 143 191 48 309.26 33.59 437 144 147 3 853.72 2.94 438 144 148 4 846.35 0.74 439 144 191 47 309.23 33.57 440 145 148 3 859.37 0.12 441 145 149 4 839.62 2.62 442 146 149 3 859.37 0.12 443 146 150 4 859.37 0.12 444 147 150 3 874.88 0.23 445 147 151 4 832.76 3.99 446 147 191 44 309.07 33.51 447 147 192 45 309.01 33.48 448 148 151 3 864.96 0.64 449 148 195 47 307.82 33.47 450 150 153 3 866.45 3.98 451 150 162 12 784.76 1.51 452 151 154 3 857.09 0.24 453 151 192 41 308.75 33.42 454 151 195 44 306.01 34.24 455 151 196 45 303.46 35.49 456 154 157 3 896.68 2.92 457 154 160 6 784.89 1.50 458 154 204 50 288.56 42.67 459 156 159 3 859.35 0.12 460 156 160 4 861.21 0.82 461 157 192 35 308.61 33.44 462 157 196 39 303.43 35.50 463 157 200 43 288.74 42.63 464 157 203 46 288.66 42.67 465 157 204 47 288.54 42.68 466 158 161 3 926.60 0.24 467 158 203 45 288.67 42.66 468 158 205 47 288.53 42.68 469 160 204 44 288.35 42.59 470 161 205 44 284.50 43.04 471 161 207 46 283.92 43.29 472 162 188 26 308.75 33.45 473 162 192 30 308.64 33.45 474 162 204 42 288.56 42.67 475 162 205 43 287.95 42.63 476 162 206 44 283.94 43.29 477 162 207 45 283.93 43.29 478 163 207 44 283.89 43.29 479 163 209 46 283.48 43.27 480 163 230 67 120.78 0.13 481 164 184 20 309.43 33.68 482 164 185 21 309.36 33.67 483 164 188 24 309.39 33.68 484 164 207 43 283.93 43.28 485 164 208 44 283.90 43.28 486 164 209 45 283.84 43.27 487 165 170 5 835.10 3.25 488 165 209 44 283.72 43.23 489 166 169 3 866.43 1.36 490 166 170 4 843.21 0.75 491 166 176 10 310.84 33.27 492 166 208 42 283.94 43.28 493 166 209 43 283.89 43.29 494 166 210 44 283.03 43.62 495 167 170 3 862.18 2.51 496 167 172 5 835.10 3.25 497 168 172 4 832.35 4.62 498 168 174 6 785.53 1.53 499 168 175 7 682.85 49.81 500 168 176 8 391.17 10.81 501 168 180 12 329.10 30.30 502 169 172 3 867.37 1.94 503 169 174 5 551.50 75.41 504 169 175 6 456.67 43.56 505 169 176 7 342.05 35.37 506 170 209 39 283.88 43.29 507 170 210 40 283.05 43.64 508 176 180 4 822.87 4.21 509 176 208 32 287.60 42.81 510 177 180 3 847.05 1.47 511 177 181 4 835.45 4.28 512 178 181 3 847.14 1.42 513 178 182 4 835.66 4.32 514 178 219 41 174.93 24.34 515 178 220 42 174.81 24.41 516 178 223 45 129.64 1.88 517 178 226 48 125.45 0.87 518 179 182 3 849.00 2.31 519 179 183 4 837.07 3.62 520 179 223 44 129.62 1.89 521 180 183 3 848.97 2.33 522 180 184 4 854.28 0.33 523 181 184 3 866.57 3.24 524 181 185 4 854.28 0.33 525 181 208 27 288.24 42.57 526 181 220 39 174.81 24.40 527 181 226 45 122.79 0.01 528 182 185 3 854.86 0.04 529 182 186 4 854.85 0.05 530 182 223 41 124.98 1.14 531 182 224 42 125.06 1.09 532 182 225 43 122.94 0.07 533 182 226 44 122.75 0.02 534 183 186 3 854.86 0.04 535 183 187 4 854.84 0.04 536 184 187 3 854.85 0.04 537 184 188 4 840.84 7.04 538 185 188 3 843.23 4.59 539 185 189 4 842.02 5.20 540 185 206 21 288.61 42.66 541 185 207 22 288.59 42.67 542 185 208 23 288.53 42.68 543 185 225 40 122.69 0.04 544 185 226 41 122.68 0.04 545 186 189 3 853.55 0.57 546 186 190 4 841.47 8.14 547 186 225 39 122.73 0.01 548 187 190 3 850.27 3.74 549 187 191 4 841.47 8.14 550 188 191 3 853.12 2.33 551 188 192 4 841.44 8.16 552 188 206 18 288.61 42.66 553 189 192 3 857.14 0.32 554 189 193 4 841.44 8.16 555 189 201 12 288.89 42.68 556 189 204 15 288.86 42.66 557 189 206 17 288.64 42.65 558 189 225 36 122.61 0.04 559 189 227 38 122.61 0.01 560 190 193 3 857.14 0.32 561 190 194 4 820.49 2.77 562 191 194 3 857.10 0.29 563 191 195 4 814.45 0.23 564 192 195 3 845.52 2.40 565 192 196 4 806.00 3.12 566 192 197 5 804.15 2.21 567 192 201 9 288.88 42.68 568 192 204 12 288.85 42.66 569 193 197 4 603.44 99.29 570 193 198 5 310.21 33.81 571 193 201 8 290.69 43.56 572 196 200 4 822.68 4.09 573 197 200 3 842.11 0.30 574 197 201 4 841.31 5.21 575 198 201 3 854.69 1.47 576 198 202 4 841.38 5.19 577 199 202 3 861.76 5.00 578 199 203 4 850.96 0.39 579 200 203 3 858.26 3.25 580 200 204 4 830.03 7.68 581 201 204 3 859.84 2.46 582 204 227 23 122.06 0.22 583 205 227 22 121.80 0.14 584 206 225 19 122.38 0.20 585 206 226 20 122.23 0.18 586 206 227 21 122.17 0.18 587 207 227 20 122.22 0.20 588 207 228 21 121.77 0.16 589 207 229 22 121.46 0.02 590 207 230 23 121.21 0.14 591 208 212 4 864.62 3.89 592 208 220 12 125.93 0.75 593 208 226 18 122.37 0.23 594 208 228 20 122.23 0.20 595 208 229 21 121.47 0.02 596 208 230 22 121.47 0.02 597 209 230 21 121.14 0.15 598 211 216 5 824.43 0.46 599 212 216 4 824.43 0.46 600 212 217 5 556.01 117.99 601 212 220 8 333.07 36.59 602 212 229 17 122.36 0.23 603 212 231 19 122.20 0.21 604 212 234 22 120.98 0.08 605 212 243 31 51.81 0.02 606 213 216 3 859.71 2.35 607 213 217 4 810.53 6.48 608 213 234 21 120.99 0.09 609 213 243 30 51.81 0.02 610 214 217 3 869.12 5.92 611 214 239 25 54.36 1.18 612 214 242 28 51.92 0.01 613 214 243 29 51.80 0.01 614 216 219 3 852.78 0.18 615 216 220 4 840.19 6.26 616 217 220 3 866.17 6.73 617 217 221 4 840.20 6.27 618 217 229 12 122.95 0.20 619 217 243 26 47.41 0.55 620 217 246 29 35.86 2.33 621 218 221 3 855.38 1.11 622 218 222 4 840.21 6.27 623 218 246 28 32.85 0.84 624 219 222 3 875.61 2.01 625 219 223 4 858.99 0.69 626 220 223 3 867.61 4.04 627 220 226 6 242.98 8.14 628 220 229 9 123.15 0.10 629 221 226 5 320.31 30.52 630 221 227 6 175.18 25.75 631 221 228 7 123.17 0.10 632 221 229 8 123.16 0.10 633 221 246 25 32.80 0.86 634 221 247 26 27.91 0.47 635 223 226 3 842.93 7.21 636 229 243 14 46.01 0.10 637 229 246 17 32.84 0.85 638 231 234 3 889.68 0.03 639 231 235 4 826.70 1.06 640 231 240 9 56.48 2.28 641 231 243 12 50.45 0.18 642 232 235 3 874.39 2.50 643 233 236 3 870.91 0.77 644 233 237 4 870.87 0.74 645 234 237 3 870.88 0.74 646 234 240 6 224.34 0.80 647 234 243 9 172.31 22.69 648 235 240 5 176.45 24.75 649 237 240 3 886.09 1.99 650 238 241 3 894.66 1.70 651 239 242 3 872.93 1.75 652 239 243 4 860.66 2.69 653 240 243 3 855.32 0.02 654 240 244 4 853.91 0.68 655 241 244 3 851.00 0.77 656 241 245 4 850.99 0.77 657 242 245 3 850.97 0.78 658 242 246 4 850.96 0.78 659 243 246 3 852.01 1.30 660 244 247 3 862.20 5.07 661 244 248 4 852.01 1.30 662 245 248 3 834.32 4.95