BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 114.35 2.23 2 3 228 225 114.06 2.06 3 3 229 226 111.70 1.07 4 4 204 200 143.77 12.31 5 4 205 201 143.74 12.29 6 4 206 202 143.73 12.29 7 4 207 203 141.47 11.28 8 4 229 225 114.58 2.11 9 5 36 31 460.70 0.28 10 5 37 32 460.70 0.28 11 5 38 33 460.69 0.28 12 5 207 202 143.78 12.31 13 6 36 30 460.83 0.31 14 6 37 31 460.73 0.27 15 6 38 32 460.70 0.28 16 6 223 217 114.86 2.15 17 6 229 223 114.84 2.14 18 6 249 243 21.66 0.64 19 7 38 31 460.72 0.27 20 7 40 33 460.73 0.29 21 7 90 83 237.86 57.11 22 7 163 156 227.21 53.84 23 7 207 200 147.22 13.92 24 7 209 202 144.01 12.43 25 7 229 222 115.06 2.06 26 7 230 223 114.86 2.14 27 7 231 224 114.85 2.14 28 8 38 30 460.82 0.32 29 8 39 31 460.74 0.28 30 8 40 32 460.74 0.28 31 8 231 223 114.88 2.14 32 8 242 234 56.16 1.25 33 8 246 238 41.36 0.65 34 9 40 31 460.74 0.28 35 9 124 115 235.59 56.21 36 9 231 222 115.09 2.05 37 9 232 223 114.86 2.14 38 9 233 224 114.83 2.14 39 9 242 233 56.32 1.30 40 10 40 30 460.83 0.32 41 10 41 31 460.82 0.32 42 10 42 32 460.82 0.32 43 10 233 223 114.88 2.14 44 10 237 227 85.40 0.07 45 10 242 232 56.32 1.30 46 11 42 31 460.82 0.32 47 11 65 54 460.06 0.01 48 11 95 84 237.21 56.91 49 11 232 221 115.62 1.93 50 11 233 222 115.40 1.89 51 11 234 223 114.84 2.14 52 11 237 226 93.40 0.75 53 12 15 3 883.02 0.58 54 12 21 9 462.47 0.62 55 12 24 12 461.85 0.32 56 12 25 13 461.67 0.42 57 12 41 29 460.92 0.29 58 12 42 30 460.88 0.30 59 12 43 31 460.88 0.30 60 12 65 53 460.54 0.22 61 12 237 225 113.58 2.02 62 12 238 226 92.39 0.24 63 13 44 31 460.94 0.33 64 13 65 52 460.36 0.29 65 13 95 82 237.74 57.09 66 13 97 84 237.66 57.12 67 13 234 221 115.89 1.79 68 14 44 30 460.94 0.32 69 15 44 29 461.09 0.36 70 15 238 223 114.72 2.17 71 16 21 5 800.14 1.99 72 16 47 31 461.20 0.35 73 17 20 3 858.01 4.27 74 17 21 4 846.89 1.27 75 18 21 3 875.83 0.18 76 18 22 4 854.30 4.96 77 18 50 32 461.56 0.43 78 19 22 3 865.38 0.58 79 19 23 4 851.00 0.80 80 20 23 3 869.99 1.72 81 20 24 4 858.41 2.90 82 21 24 3 858.41 2.90 83 21 25 4 850.97 0.82 84 21 47 26 461.50 0.42 85 21 50 29 462.70 0.49 86 21 238 217 112.58 2.19 87 22 25 3 859.81 2.25 88 22 26 4 850.97 0.83 89 22 54 32 463.35 0.34 90 23 26 3 858.70 2.75 91 23 27 4 854.25 1.93 92 24 27 3 861.83 1.86 93 24 28 4 854.26 1.92 94 24 237 213 112.78 2.27 95 24 238 214 98.88 4.62 96 24 239 215 90.77 2.07 97 25 28 3 866.21 4.06 98 25 29 4 847.45 1.49 99 25 51 26 463.48 0.53 100 25 54 29 463.76 0.48 101 25 55 30 463.75 0.49 102 26 29 3 859.36 3.10 103 26 30 4 851.48 0.53 104 26 54 28 463.97 0.60 105 27 30 3 862.53 3.17 106 27 31 4 855.03 1.25 107 27 240 213 75.76 0.40 108 27 243 216 62.73 1.59 109 28 31 3 856.86 0.34 110 28 41 13 463.64 0.48 111 28 55 27 464.15 0.65 112 28 57 29 464.15 0.66 113 28 60 32 464.03 0.62 114 28 242 214 58.71 0.19 115 28 243 215 57.47 0.73 116 29 54 25 464.13 0.67 117 29 55 26 464.13 0.67 118 29 56 27 464.14 0.66 119 29 57 28 464.14 0.66 120 31 57 26 483.28 8.88 121 31 243 212 56.13 1.22 122 33 37 4 859.93 3.22 123 33 39 6 521.27 2.46 124 33 57 24 549.85 7.74 125 33 59 26 518.07 0.86 126 33 60 27 518.06 0.87 127 33 246 213 49.69 0.34 128 34 59 25 558.02 3.51 129 35 59 24 568.37 1.97 130 35 246 211 47.43 0.75 131 35 247 212 46.02 0.14 132 35 249 214 39.05 0.09 133 35 250 215 25.68 1.40 134 37 59 22 568.65 2.07 135 37 246 209 44.11 0.63 136 37 249 212 27.77 0.01 137 38 59 21 568.66 2.08 138 38 60 22 568.63 2.06 139 38 61 23 568.62 2.06 140 38 90 52 238.21 57.29 141 39 59 20 568.71 2.09 142 39 60 21 568.63 2.07 143 39 61 22 568.64 2.06 144 39 246 207 44.05 0.69 145 40 61 21 568.65 2.07 146 40 63 23 568.55 2.06 147 40 90 50 238.21 57.28 148 40 124 84 234.81 56.55 149 41 51 10 568.98 2.00 150 41 55 14 568.95 2.04 151 41 60 19 568.85 2.06 152 41 61 20 568.80 2.04 153 41 62 21 568.59 2.06 154 41 63 22 568.58 2.06 155 42 63 21 568.58 2.06 156 42 65 23 568.25 1.93 157 42 92 50 238.28 57.25 158 43 47 4 841.62 4.29 159 43 48 5 569.34 2.10 160 43 51 8 569.33 2.09 161 43 63 20 568.83 2.06 162 43 65 22 568.57 2.04 163 44 47 3 859.12 0.62 164 44 48 4 854.03 1.92 165 44 65 21 568.67 2.07 166 45 48 3 871.48 5.44 167 45 64 19 568.85 2.06 168 45 66 21 568.75 2.06 169 45 78 33 247.47 56.15 170 47 50 3 860.26 1.19 171 47 51 4 837.74 3.09 172 48 51 3 848.75 0.70 173 48 52 4 843.19 0.36 174 48 62 14 568.91 2.05 175 48 63 15 568.90 2.05 176 48 83 35 244.21 56.02 177 48 86 38 242.41 56.91 178 48 88 40 241.16 57.18 179 49 52 3 859.57 7.78 180 49 53 4 845.92 1.01 181 49 86 37 244.21 56.01 182 50 53 3 860.40 7.36 183 50 54 4 840.82 3.52 184 51 54 3 869.57 2.78 185 51 55 4 761.08 36.30 186 51 62 11 568.93 2.06 187 52 62 10 568.95 2.05 188 52 86 34 242.40 56.91 189 55 60 5 569.13 2.07 190 55 62 7 569.04 2.10 191 57 60 3 860.80 0.81 192 60 246 186 46.62 1.84 193 61 90 29 238.23 57.29 194 62 88 26 239.05 57.53 195 62 90 28 238.26 57.30 196 63 88 25 239.63 57.27 197 63 90 27 238.31 57.28 198 63 91 28 238.31 57.28 199 64 83 19 242.16 56.80 200 64 88 24 240.08 57.27 201 64 90 26 238.88 57.46 202 64 91 27 238.44 57.26 203 64 92 28 238.44 57.25 204 65 92 27 238.46 57.25 205 66 91 25 239.93 57.19 206 66 92 26 239.75 57.16 207 66 93 27 239.42 57.26 208 66 98 32 239.46 57.10 209 66 112 46 242.31 56.00 210 67 78 11 247.47 56.18 211 67 80 13 247.37 56.20 212 67 83 16 247.35 56.19 213 67 91 24 240.81 56.91 214 67 93 26 240.02 57.14 215 67 112 45 242.34 56.02 216 68 74 6 341.56 9.61 217 68 77 9 247.71 56.14 218 68 78 10 247.67 56.15 219 68 79 11 247.51 56.16 220 68 80 12 247.44 56.20 221 68 112 44 244.04 55.17 222 69 79 10 247.64 56.15 223 69 80 11 247.61 56.16 224 69 81 12 247.68 56.12 225 69 115 46 244.08 55.16 226 70 74 4 897.06 2.62 227 70 79 9 247.70 56.14 228 71 74 3 974.72 3.38 229 71 79 8 247.69 56.16 230 71 81 10 247.71 56.13 231 71 82 11 247.72 56.14 232 74 77 3 859.93 2.68 233 78 82 4 852.34 4.37 234 78 83 5 836.27 1.97 235 79 82 3 860.23 2.77 236 79 83 4 852.34 4.37 237 80 83 3 857.89 1.59 238 80 84 4 836.30 1.98 239 80 91 11 351.44 5.18 240 80 115 35 471.74 1.02 241 80 116 36 351.74 5.06 242 80 121 41 486.30 0.65 243 81 84 3 861.84 1.90 244 81 85 4 843.73 1.74 245 82 85 3 855.27 2.46 246 82 86 4 836.29 1.98 247 83 86 3 847.00 3.38 248 83 87 4 833.74 3.25 249 83 88 5 738.38 31.62 250 83 91 8 486.98 0.94 251 83 121 38 578.71 1.07 252 84 87 3 828.12 9.25 253 84 88 4 809.76 4.07 254 84 119 35 605.41 2.68 255 84 121 37 588.28 5.87 256 88 121 33 659.07 0.81 257 89 121 32 659.07 0.80 258 89 122 33 659.06 0.80 259 90 121 31 659.09 0.80 260 90 122 32 659.07 0.80 261 90 124 34 659.05 0.80 262 90 163 73 659.07 0.80 263 91 116 25 659.18 0.81 264 91 121 30 659.15 0.82 265 91 122 31 659.12 0.81 266 91 123 32 659.08 0.80 267 91 124 33 659.07 0.80 268 92 95 3 916.16 0.19 269 92 124 32 659.07 0.81 270 92 126 34 659.05 0.81 271 92 232 140 159.13 19.16 272 93 112 19 659.25 0.81 273 93 113 20 659.20 0.82 274 93 116 23 659.34 0.77 275 93 123 30 659.15 0.82 276 93 124 31 659.12 0.82 277 93 125 32 659.07 0.80 278 93 126 33 659.06 0.80 279 94 99 5 816.77 2.23 280 94 104 10 659.26 0.85 281 94 109 15 659.19 0.82 282 94 126 32 659.07 0.80 283 95 98 3 855.48 0.73 284 95 99 4 821.15 0.07 285 95 126 31 659.08 0.81 286 95 167 72 659.03 0.81 287 95 232 137 159.63 18.95 288 96 99 3 850.90 1.55 289 96 100 4 821.13 0.07 290 96 167 71 659.02 0.79 291 96 169 73 654.44 0.36 292 97 100 3 857.85 0.14 293 97 101 4 821.14 0.07 294 97 102 5 821.11 0.07 295 98 101 3 864.41 0.80 296 98 102 4 819.96 0.49 297 98 103 5 819.96 0.49 298 98 104 6 660.94 1.34 299 99 104 5 660.92 1.35 300 99 106 7 660.94 1.35 301 99 109 10 660.89 1.36 302 99 126 27 659.33 0.78 303 99 127 28 659.32 0.78 304 99 146 47 659.35 0.79 305 103 108 5 846.00 1.16 306 104 108 4 842.69 2.81 307 104 109 5 828.59 4.25 308 104 112 8 827.83 4.56 309 105 108 3 875.35 4.82 310 105 109 4 846.00 1.16 311 106 109 3 875.35 4.82 312 106 110 4 844.94 1.69 313 106 145 39 659.62 0.80 314 106 146 40 659.50 0.83 315 106 149 43 659.63 0.80 316 107 110 3 846.19 1.25 317 107 111 4 845.12 1.78 318 108 111 3 861.97 2.08 319 108 112 4 845.21 1.74 320 109 112 3 861.48 2.33 321 109 113 4 845.39 1.65 322 109 150 41 659.64 0.80 323 110 113 3 868.64 1.26 324 110 114 4 845.34 1.61 325 110 149 39 659.66 0.81 326 110 150 40 659.63 0.80 327 110 153 43 660.25 0.71 328 111 114 3 870.57 0.29 329 111 115 4 848.16 3.00 330 111 153 42 660.29 0.69 331 112 115 3 854.92 4.26 332 112 116 4 845.35 1.61 333 113 116 3 856.25 3.35 334 113 117 4 843.68 2.95 335 113 123 10 659.96 0.72 336 113 125 12 659.62 0.80 337 113 150 37 660.00 0.72 338 113 154 41 660.47 0.76 339 114 117 3 855.76 3.97 340 114 118 4 846.78 1.40 341 114 153 39 660.30 0.68 342 114 154 40 660.32 0.69 343 115 118 3 848.79 0.39 344 115 119 4 847.13 1.57 345 116 119 3 868.61 1.25 346 116 120 4 847.13 1.56 347 116 121 5 666.28 1.51 348 116 123 7 660.34 0.69 349 117 120 3 856.94 4.57 350 117 123 6 660.48 0.75 351 117 154 37 673.16 1.79 352 117 158 41 667.21 1.06 353 117 161 44 669.09 0.11 354 117 162 45 665.71 1.80 355 120 161 41 784.49 1.85 356 121 161 40 784.53 1.86 357 122 160 38 784.60 1.84 358 122 161 39 784.52 1.86 359 123 161 38 784.59 1.85 360 123 162 39 784.53 1.85 361 123 163 40 784.52 1.85 362 124 163 39 784.53 1.85 363 124 165 41 784.46 1.84 364 124 209 85 242.19 24.57 365 125 150 25 784.77 1.88 366 125 163 38 784.58 1.85 367 125 164 39 784.53 1.85 368 125 165 40 784.52 1.85 369 126 165 39 784.53 1.85 370 126 167 41 784.49 1.85 371 126 232 106 159.63 18.95 372 127 143 16 785.05 1.91 373 127 146 19 784.98 1.88 374 127 147 20 784.97 1.87 375 127 150 23 784.97 1.87 376 127 164 37 784.62 1.84 377 127 165 38 784.58 1.84 378 127 166 39 784.52 1.85 379 127 167 40 784.51 1.85 380 127 190 63 249.02 23.66 381 128 143 15 785.09 1.90 382 128 146 18 785.08 1.90 383 128 166 38 784.57 1.85 384 128 167 39 784.52 1.85 385 128 169 41 784.44 1.85 386 129 133 4 830.13 5.58 387 129 134 5 824.92 8.18 388 129 139 10 785.26 1.90 389 129 142 13 785.18 1.89 390 129 166 37 784.63 1.84 391 129 169 40 784.48 1.84 392 129 170 41 784.44 1.85 393 130 133 3 865.63 1.50 394 130 134 4 830.20 5.56 395 130 169 39 784.45 1.84 396 130 170 40 784.44 1.85 397 131 134 3 851.51 2.48 398 131 135 4 830.20 5.56 399 131 170 39 784.34 1.80 400 131 174 43 737.93 2.15 401 131 176 45 697.82 0.76 402 132 135 3 853.93 1.48 403 132 136 4 848.89 3.79 404 133 136 3 862.05 2.58 405 133 137 4 850.14 3.16 406 133 138 5 785.75 2.00 407 133 139 6 785.59 1.91 408 133 142 9 785.53 1.91 409 134 137 3 841.02 1.40 410 134 139 5 785.57 1.92 411 134 166 32 784.70 1.83 412 134 170 36 784.36 1.79 413 134 182 48 263.16 17.18 414 138 141 3 846.66 0.67 415 138 142 4 843.82 2.06 416 139 142 3 863.18 4.67 417 139 143 4 843.85 2.07 418 140 143 3 872.50 1.93 419 140 144 4 843.57 2.21 420 140 189 49 249.25 23.68 421 141 145 4 839.28 0.03 422 142 145 3 844.46 2.62 423 142 146 4 840.37 0.50 424 143 146 3 849.77 2.20 425 143 147 4 842.38 1.51 426 143 166 23 784.62 1.85 427 143 190 47 249.12 23.64 428 144 147 3 851.60 1.69 429 144 148 4 842.38 1.51 430 144 190 46 249.11 23.64 431 144 193 49 249.00 23.66 432 145 148 3 856.97 2.35 433 145 149 4 857.01 2.32 434 146 149 3 864.53 1.30 435 146 150 4 857.03 2.32 436 147 150 3 857.03 2.32 437 147 151 4 857.02 2.32 438 147 159 12 785.51 1.73 439 147 193 46 248.94 23.62 440 148 151 3 862.99 5.30 441 148 152 4 843.73 4.33 442 148 193 45 248.94 23.62 443 149 152 3 868.72 0.04 444 150 153 3 875.27 1.06 445 150 154 4 860.57 4.12 446 151 154 3 864.86 6.27 447 151 159 8 786.16 1.63 448 151 193 42 248.91 23.62 449 151 198 47 247.56 23.05 450 154 158 4 841.48 3.40 451 154 159 5 841.12 3.58 452 154 162 8 785.85 1.82 453 155 158 3 862.15 3.72 454 155 159 4 868.66 0.47 455 156 159 3 878.37 2.96 456 156 198 42 244.03 24.61 457 156 201 45 243.77 24.67 458 156 202 46 243.58 24.59 459 157 160 3 883.29 0.50 460 158 161 3 873.36 0.45 461 158 162 4 858.75 0.11 462 159 162 3 853.24 1.74 463 159 164 5 855.35 2.79 464 159 198 39 244.11 24.75 465 159 202 43 243.36 24.55 466 159 206 47 242.68 24.54 467 162 206 44 242.65 24.52 468 163 207 44 242.30 24.55 469 163 209 46 242.26 24.55 470 164 190 26 248.84 23.65 471 164 206 42 242.78 24.55 472 164 207 43 242.40 24.57 473 164 208 44 242.28 24.55 474 164 209 45 242.28 24.55 475 165 209 44 242.25 24.54 476 165 232 67 159.63 18.95 477 166 186 20 249.12 23.73 478 166 187 21 248.99 23.69 479 166 190 24 249.00 23.68 480 166 209 43 242.29 24.55 481 166 210 44 242.24 24.54 482 166 211 45 242.19 24.57 483 168 171 3 877.06 0.10 484 168 172 4 868.84 0.84 485 168 210 42 242.30 24.55 486 168 211 43 242.25 24.53 487 168 212 44 242.12 24.55 488 169 172 3 868.83 0.84 489 169 174 5 847.34 9.90 490 170 174 4 847.98 9.58 491 170 176 6 542.40 77.70 492 170 178 8 277.36 19.65 493 170 182 12 263.11 17.16 494 171 174 3 858.91 2.79 495 171 176 5 524.34 77.09 496 171 178 7 263.26 17.22 497 172 212 40 242.19 24.57 498 178 182 4 844.07 0.88 499 178 210 32 242.97 24.48 500 179 182 3 860.45 2.96 501 179 183 4 843.23 1.30 502 180 183 3 852.02 0.96 503 180 184 4 847.28 3.33 504 180 221 41 173.62 24.64 505 180 222 42 165.16 20.41 506 180 225 45 164.62 20.25 507 181 184 3 851.77 1.08 508 181 185 4 851.76 1.09 509 182 185 3 851.77 1.08 510 182 186 4 851.76 1.08 511 183 186 3 867.97 4.82 512 183 187 4 857.19 1.64 513 183 210 27 243.03 24.45 514 183 222 39 164.71 20.25 515 184 187 3 855.76 2.35 516 184 188 4 850.28 0.97 517 184 225 41 162.63 19.56 518 184 226 42 162.66 19.57 519 184 227 43 162.52 19.55 520 185 188 3 861.18 0.24 521 185 189 4 856.95 2.35 522 186 189 3 859.36 1.16 523 186 190 4 858.12 1.18 524 187 190 3 866.02 2.18 525 187 191 4 850.90 4.11 526 187 208 21 243.28 24.36 527 187 209 22 243.28 24.36 528 187 210 23 243.25 24.34 529 187 227 40 162.37 19.55 530 187 228 41 162.36 19.55 531 188 191 3 858.13 1.18 532 188 192 4 838.38 2.18 533 188 227 39 162.41 19.57 534 189 192 3 864.80 2.83 535 189 193 4 842.82 4.41 536 190 193 3 864.82 2.81 537 190 194 4 838.15 2.31 538 190 208 18 243.31 24.37 539 191 194 3 860.44 2.41 540 191 195 4 838.14 2.31 541 191 203 12 257.79 17.95 542 191 206 15 243.65 24.43 543 191 208 17 243.46 24.43 544 191 227 36 162.38 19.55 545 191 229 38 162.38 19.55 546 192 195 3 858.90 7.12 547 192 196 4 850.14 2.75 548 193 196 3 858.09 0.72 549 193 197 4 844.58 5.53 550 193 198 5 844.58 5.53 551 194 197 3 856.57 1.18 552 194 198 4 837.19 1.83 553 194 199 5 837.18 1.83 554 194 202 8 362.10 9.11 555 194 203 9 265.42 19.81 556 194 206 12 257.29 17.74 557 195 198 3 869.88 3.71 558 195 199 4 816.11 8.00 559 195 200 5 433.12 34.15 560 195 203 8 266.48 21.99 561 198 202 4 832.59 3.00 562 199 202 3 854.15 0.43 563 199 203 4 837.33 2.54 564 200 203 3 849.01 3.00 565 200 204 4 837.33 2.54 566 201 204 3 855.67 0.16 567 201 205 4 841.90 0.26 568 202 205 3 858.83 1.48 569 202 206 4 834.48 3.97 570 203 206 3 855.00 0.18 571 206 229 23 161.91 19.59 572 207 229 22 161.89 19.59 573 208 227 19 162.07 19.64 574 208 228 20 161.94 19.59 575 208 229 21 161.90 19.58 576 209 229 20 161.84 19.54 577 209 230 21 160.41 18.93 578 209 231 22 160.09 18.91 579 209 232 23 160.08 18.91 580 210 214 4 874.61 0.95 581 210 222 12 164.27 20.39 582 210 228 18 161.96 19.59 583 210 230 20 161.19 19.21 584 210 231 21 160.11 18.89 585 210 232 22 160.12 18.90 586 211 232 21 160.03 18.86 587 213 218 5 830.41 0.10 588 214 218 4 832.94 1.37 589 214 219 5 652.10 73.05 590 214 222 8 293.44 17.89 591 214 231 17 160.86 19.05 592 214 233 19 160.43 18.89 593 214 236 22 160.13 18.80 594 214 245 31 98.25 22.45 595 215 218 3 875.88 10.88 596 215 219 4 827.63 1.38 597 215 236 21 160.15 18.80 598 215 241 26 109.95 28.09 599 215 245 30 98.27 22.46 600 216 219 3 856.65 1.71 601 216 241 25 119.48 32.86 602 216 244 28 98.45 22.41 603 216 245 29 98.28 22.46 604 217 241 24 126.26 34.93 605 218 221 3 872.41 0.35 606 218 222 4 841.09 0.58 607 219 222 3 857.18 3.13 608 219 223 4 841.47 0.78 609 219 231 12 162.31 19.70 610 219 245 26 51.16 0.72 611 219 248 29 40.85 0.92 612 220 223 3 858.20 1.60 613 220 224 4 835.54 3.37 614 220 248 28 40.84 0.92 615 220 250 30 40.83 0.92 616 221 224 3 858.50 1.45 617 221 225 4 835.55 3.37 618 222 225 3 881.82 1.06 619 222 228 6 278.43 24.77 620 222 231 9 207.94 2.96 621 223 228 5 271.65 28.86 622 223 229 6 270.94 28.51 623 223 230 7 207.95 2.96 624 223 231 8 207.95 2.96 625 223 248 25 40.81 0.92 626 223 249 26 33.95 2.49 627 225 228 3 854.75 0.29 628 228 231 3 1059.61 0.64 629 231 245 14 46.47 0.28 630 231 249 18 28.80 0.55 631 233 236 3 902.15 1.00 632 233 237 4 825.14 0.35 633 233 242 9 203.96 0.42 634 233 245 12 53.36 0.92 635 234 237 3 863.50 1.05 636 235 238 3 872.41 3.41 637 236 239 3 860.84 4.70 638 236 241 5 281.64 19.74 639 236 242 6 269.37 15.51 640 236 245 9 209.22 3.00 641 237 242 5 273.77 17.71 642 239 242 3 871.74 0.26 643 240 243 3 871.08 3.85 644 241 244 3 855.87 1.28 645 241 245 4 842.09 1.22 646 242 245 3 850.55 2.71 647 242 246 4 849.99 2.75 648 243 246 3 859.56 3.06 649 243 247 4 850.00 2.74 650 244 247 3 862.45 4.45 651 244 248 4 850.00 2.74 652 245 248 3 854.45 0.52 653 245 249 4 846.75 4.37 654 246 249 3 862.38 0.43 655 247 250 3 872.42 0.59