BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 672 676 4 845.44 5.58 2 673 676 3 858.46 0.93 3 673 677 4 845.44 5.58 4 673 723 50 27.41 1.12 5 673 806 133 24.23 0.01 6 673 834 161 24.18 0.01 7 673 837 164 23.73 0.26 8 674 677 3 855.50 0.55 9 674 678 4 833.41 3.13 10 674 802 128 24.87 0.17 11 674 841 167 26.50 1.11 12 675 678 3 849.23 4.11 13 675 679 4 828.36 5.66 14 676 679 3 833.41 3.14 15 676 680 4 828.14 5.76 16 676 723 47 28.80 0.93 17 677 680 3 838.27 0.82 18 677 681 4 823.59 4.54 19 677 722 45 30.94 0.10 20 677 805 128 28.61 0.85 21 677 806 129 27.73 1.29 22 677 809 132 27.94 1.19 23 678 681 3 834.07 3.10 24 678 802 124 30.78 0.20 25 678 805 127 30.71 0.17 26 680 718 38 31.54 0.14 27 680 721 41 31.53 0.12 28 680 722 42 31.53 0.12 29 681 718 37 31.71 0.17 30 681 805 124 31.46 0.15 31 681 808 127 31.49 0.17 32 682 714 32 37.02 0.46 33 682 718 36 33.62 0.78 34 684 766 82 86.24 0.17 35 685 707 22 86.24 0.16 36 685 710 25 85.91 0.06 37 685 711 26 77.50 0.53 38 685 714 29 77.40 0.54 39 685 765 80 86.07 0.25 40 685 766 81 86.27 0.18 41 686 707 21 86.25 0.17 42 686 710 24 86.25 0.16 43 686 765 79 86.20 0.18 44 686 766 80 86.27 0.18 45 686 767 81 86.28 0.17 46 687 706 19 86.28 0.17 47 687 707 20 86.25 0.17 48 687 710 23 86.27 0.17 49 687 765 78 86.28 0.18 50 687 767 80 86.39 0.23 51 687 768 81 86.29 0.18 52 688 710 22 86.40 0.21 53 688 767 79 86.47 0.20 54 689 699 10 86.56 0.21 55 689 700 11 86.55 0.21 56 689 703 14 86.48 0.18 57 689 767 78 86.51 0.20 58 689 768 79 86.50 0.20 59 689 774 85 86.56 0.21 60 690 693 3 856.28 3.29 61 691 767 76 86.60 0.19 62 691 774 83 96.63 1.15 63 695 700 5 794.68 3.15 64 695 773 78 130.23 0.30 65 695 774 79 129.72 0.14 66 695 777 82 130.32 0.32 67 696 699 3 844.18 4.71 68 696 700 4 826.47 4.14 69 696 777 81 130.43 0.32 70 697 700 3 844.18 4.71 71 697 701 4 830.76 1.99 72 697 777 80 130.66 0.21 73 697 778 81 130.49 0.29 74 697 779 82 130.49 0.29 75 697 876 179 131.27 0.14 76 697 880 183 145.55 1.03 77 697 887 190 148.53 0.03 78 697 889 192 148.52 0.03 79 698 701 3 852.20 0.70 80 698 702 4 830.66 1.94 81 698 887 189 148.53 0.03 82 698 888 190 148.56 0.03 83 698 889 191 148.44 0.01 84 699 702 3 833.87 0.43 85 699 703 4 830.71 1.96 86 700 703 3 833.14 0.76 87 700 704 4 833.09 0.72 88 700 770 70 148.55 0.03 89 700 774 74 148.56 0.03 90 700 777 77 148.59 0.03 91 700 778 78 148.58 0.03 92 701 704 3 833.08 0.72 93 701 705 4 833.09 0.71 94 701 778 77 148.61 0.03 95 701 780 79 148.59 0.03 96 701 876 175 148.59 0.02 97 701 880 179 148.60 0.03 98 701 889 188 150.00 0.18 99 702 705 3 844.03 4.76 100 702 706 4 833.08 0.71 101 702 889 187 150.00 0.18 102 702 890 188 150.00 0.18 103 703 706 3 844.06 4.75 104 703 707 4 833.07 0.70 105 703 768 65 150.34 0.27 106 704 707 3 844.34 6.33 107 704 708 4 833.06 0.70 108 704 764 60 150.34 0.27 109 704 768 64 155.45 0.38 110 704 770 66 153.00 0.54 111 704 780 76 155.93 0.18 112 705 708 3 830.66 0.72 113 705 709 4 830.65 0.72 114 705 889 184 165.87 0.44 115 705 890 185 162.08 1.44 116 705 891 186 161.22 1.27 117 705 892 187 161.50 1.17 118 705 895 190 158.09 1.46 119 706 709 3 836.62 2.27 120 706 710 4 830.63 0.72 121 706 892 186 162.97 1.08 122 707 710 3 835.85 1.89 123 707 711 4 824.83 3.62 124 707 764 57 159.97 1.44 125 707 765 58 160.46 1.65 126 707 768 61 161.21 1.27 127 708 711 3 835.85 1.89 128 708 712 4 824.86 3.60 129 708 745 37 160.42 1.66 130 708 895 187 169.46 0.21 131 709 712 3 834.73 1.48 132 709 713 4 829.88 0.62 133 709 892 183 170.46 0.17 134 709 893 184 170.75 0.31 135 709 894 185 170.47 0.21 136 709 895 186 169.91 0.01 137 710 713 3 854.02 0.24 138 710 714 4 830.65 0.57 139 711 714 3 840.30 1.48 140 711 715 4 830.20 0.79 141 711 745 34 170.16 0.22 142 712 715 3 832.61 0.74 143 712 716 4 832.60 0.74 144 712 737 25 171.24 0.48 145 712 741 29 170.50 0.22 146 712 745 33 170.49 0.22 147 712 894 182 171.81 0.45 148 712 895 183 171.77 0.43 149 712 898 186 171.78 0.43 150 713 716 3 841.52 0.05 151 713 717 4 832.60 0.76 152 713 894 181 171.88 0.43 153 714 717 3 832.55 0.78 154 714 718 4 829.65 0.58 155 715 718 3 826.50 3.81 156 715 719 4 816.23 2.08 157 715 744 29 171.77 0.45 158 715 745 30 171.78 0.45 159 716 719 3 833.68 0.22 160 716 720 4 816.24 2.08 161 716 737 21 178.93 0.97 162 717 720 3 833.97 4.95 163 717 721 4 824.36 0.15 164 718 721 3 831.34 3.33 165 718 722 4 824.36 0.15 166 718 744 26 184.40 0.52 167 718 808 90 184.47 0.55 168 718 809 91 184.39 0.52 169 718 812 94 184.49 0.54 170 719 722 3 832.70 5.01 171 719 725 6 645.25 4.38 172 719 737 18 184.69 0.44 173 719 812 93 184.49 0.54 174 720 725 5 645.25 4.38 175 720 726 6 421.00 116.48 176 722 725 3 841.43 5.54 177 722 809 87 187.22 0.33 178 722 812 90 187.36 0.30 179 722 813 91 187.41 0.32 180 722 834 112 187.40 0.32 181 723 830 107 187.41 0.33 182 723 834 111 187.41 0.33 183 724 727 3 827.34 1.97 184 724 830 106 187.43 0.33 185 725 728 3 831.84 2.18 186 725 733 8 260.27 35.80 187 725 736 11 260.24 35.79 188 725 737 12 188.24 0.23 189 725 812 87 187.56 0.35 190 725 813 88 187.56 0.35 191 725 830 105 269.70 40.73 192 726 733 7 747.07 19.30 193 727 826 99 287.53 49.42 194 728 732 4 825.00 2.17 195 728 733 5 811.03 4.80 196 728 813 85 287.13 49.19 197 728 830 102 287.03 49.25 198 729 732 3 839.36 4.52 199 729 733 4 825.49 2.42 200 729 822 93 341.95 58.98 201 729 823 94 338.25 57.93 202 730 733 3 839.87 4.26 203 730 734 4 826.00 2.66 204 731 734 3 849.40 0.51 205 731 735 4 825.12 2.21 206 731 822 91 348.60 56.34 207 732 735 3 830.44 0.05 208 732 736 4 825.12 2.21 209 732 821 89 348.62 56.43 210 732 822 90 348.62 56.33 211 732 823 91 348.74 56.27 212 733 736 3 850.68 3.46 213 733 737 4 825.12 2.22 214 734 737 3 837.93 2.52 215 734 738 4 824.94 2.27 216 735 738 3 836.24 3.37 217 735 739 4 824.96 2.29 218 735 821 86 357.82 54.45 219 736 739 3 836.72 3.59 220 736 740 4 824.96 2.29 221 736 812 76 354.45 56.10 222 736 816 80 354.50 56.12 223 736 821 85 363.32 52.05 224 736 827 91 355.92 55.38 225 737 740 3 847.60 8.65 226 737 741 4 822.80 3.35 227 737 812 75 359.58 53.49 228 738 741 3 844.42 1.02 229 738 898 160 271.93 2.10 230 738 902 164 264.97 0.55 231 739 815 76 364.07 52.12 232 739 817 78 365.01 51.83 233 739 902 163 271.06 2.48 234 739 905 166 271.05 2.48 235 739 906 167 268.75 3.62 236 739 911 172 260.04 0.48 237 740 743 3 842.97 2.67 238 740 744 4 823.12 2.23 239 740 811 71 364.49 51.91 240 740 812 72 364.48 51.90 241 740 815 75 364.64 51.97 242 741 744 3 839.41 4.45 243 741 745 4 824.73 1.44 244 741 895 154 284.11 0.40 245 741 898 157 282.32 0.94 246 741 899 158 282.88 0.70 247 741 903 162 277.13 1.04 248 742 745 3 848.76 7.13 249 742 746 4 828.20 3.16 250 742 870 128 337.38 31.16 251 742 873 131 331.33 28.05 252 742 874 132 329.56 27.25 253 742 877 135 313.60 14.38 254 743 746 3 854.96 4.03 255 743 747 4 827.62 3.45 256 743 811 68 372.01 48.58 257 743 866 123 340.79 32.98 258 743 870 127 338.17 31.55 259 744 747 3 855.56 4.33 260 744 748 4 826.58 3.98 261 744 808 64 372.11 48.52 262 744 811 67 372.11 48.52 263 744 812 68 372.11 48.52 264 745 748 3 855.32 4.45 265 745 749 4 823.20 5.67 266 746 749 3 826.45 4.04 267 746 750 4 822.95 5.55 268 746 787 41 466.02 89.42 269 746 790 44 465.94 89.39 270 746 869 123 346.76 29.87 271 746 873 127 343.76 28.33 272 747 750 3 845.93 5.84 273 747 751 4 839.50 2.72 274 747 790 43 465.94 89.37 275 747 794 47 465.94 89.38 276 747 804 57 378.87 45.86 277 747 807 60 378.81 45.89 278 747 808 61 378.80 45.88 279 747 811 64 378.75 45.94 280 747 862 115 350.09 31.53 281 747 866 119 347.10 30.04 282 748 751 3 839.77 2.58 283 748 752 4 834.59 0.75 284 748 804 56 378.91 45.85 285 748 808 60 378.82 45.88 286 749 752 3 848.09 1.58 287 749 753 4 835.57 0.27 288 749 762 13 468.70 89.15 289 749 763 14 468.73 89.14 290 749 764 15 468.42 88.97 291 749 787 38 467.44 88.71 292 750 753 3 835.58 0.27 293 750 754 4 835.57 0.27 294 750 791 41 467.09 88.81 295 750 794 44 467.51 88.59 296 751 754 3 840.50 2.19 297 751 755 4 836.46 4.21 298 751 794 43 467.51 88.59 299 751 799 48 381.25 45.47 300 751 800 49 381.25 45.47 301 751 801 50 381.23 45.47 302 751 804 53 379.96 45.34 303 752 755 3 848.99 1.09 304 752 756 4 831.47 1.77 305 752 763 11 468.78 89.15 306 752 764 12 468.81 89.14 307 753 757 4 806.47 10.70 308 753 758 5 693.40 15.09 309 753 759 6 605.31 21.25 310 753 761 8 469.04 89.26 311 753 762 9 468.91 89.23 312 753 763 10 468.79 89.14 313 753 791 38 467.61 88.59 314 754 758 4 653.96 5.70 315 754 759 5 605.33 21.24 316 754 791 37 467.61 88.59 317 754 794 40 467.61 88.59 318 754 795 41 467.56 88.57 319 756 763 7 469.19 89.18 320 757 760 3 837.56 3.87 321 757 761 4 792.89 11.79 322 757 763 6 469.31 89.13 323 757 769 12 469.06 88.89 324 759 788 29 467.82 88.56 325 759 791 32 467.69 88.61 326 759 792 33 467.64 88.58 327 760 788 28 468.06 88.46 328 761 769 8 469.17 88.81 329 761 770 9 469.11 88.79 330 761 771 10 469.04 88.75 331 762 769 7 469.16 88.81 332 762 770 8 469.09 88.78 333 762 775 13 469.00 88.74 334 762 784 22 468.18 88.41 335 762 787 25 468.18 88.41 336 762 788 26 468.12 88.38 337 763 766 3 899.06 1.99 338 763 769 6 469.12 88.78 339 764 768 4 789.09 15.61 340 764 770 6 648.01 0.62 341 764 787 23 468.20 88.39 342 765 768 3 811.71 4.30 343 765 769 4 809.58 5.37 344 766 769 3 884.74 6.25 345 769 774 5 834.40 7.74 346 770 774 4 807.98 5.47 347 770 775 5 806.33 4.69 348 770 778 8 751.19 23.47 349 770 784 14 469.34 87.86 350 771 774 3 845.38 4.96 351 771 775 4 842.71 3.60 352 772 775 3 845.42 4.95 353 772 776 4 845.42 4.96 354 772 781 9 601.88 23.27 355 772 788 16 469.79 87.69 356 773 776 3 856.44 0.52 357 773 777 4 845.41 4.96 358 774 777 3 850.60 2.37 359 774 778 4 842.69 3.60 360 775 778 3 847.86 1.00 361 775 780 5 732.35 19.32 362 775 781 6 591.08 27.09 363 775 784 9 591.01 27.07 364 775 788 13 588.52 28.33 365 776 779 3 840.07 2.90 366 776 781 5 601.87 23.28 367 778 876 98 374.99 14.93 368 779 876 97 375.00 14.93 369 780 783 3 856.19 0.51 370 780 784 4 828.85 3.00 371 780 787 7 819.38 7.81 372 780 873 93 375.63 15.24 373 780 876 96 374.97 14.91 374 781 784 3 859.10 6.51 375 781 785 4 840.52 2.79 376 782 785 3 854.70 0.24 377 782 786 4 844.61 4.80 378 783 786 3 855.57 0.21 379 783 787 4 841.35 2.39 380 783 872 89 376.03 15.30 381 783 873 90 375.47 15.02 382 783 876 93 375.31 14.75 383 784 787 3 845.43 4.40 384 784 788 4 841.38 2.38 385 785 788 3 841.39 2.38 386 785 789 4 841.41 2.36 387 786 789 3 841.41 2.36 388 786 790 4 841.42 2.36 389 786 868 82 379.10 16.62 390 786 869 83 378.21 16.18 391 786 872 86 376.31 15.22 392 787 790 3 841.56 2.29 393 787 791 4 830.09 3.31 394 787 869 82 378.05 16.12 395 787 873 86 375.80 14.81 396 788 791 3 830.24 3.37 397 788 792 4 830.16 3.27 398 789 792 3 837.24 5.33 399 789 793 4 829.51 2.95 400 789 796 7 817.25 3.10 401 790 793 3 834.38 5.38 402 790 794 4 840.57 2.38 403 790 862 72 379.83 16.84 404 790 866 76 379.56 16.75 405 790 869 79 378.38 16.09 406 791 794 3 845.25 0.04 407 791 795 4 840.59 2.38 408 792 795 3 859.23 6.74 409 792 796 4 835.86 4.74 410 793 796 3 859.23 6.74 411 793 797 4 834.60 4.11 412 793 862 69 379.87 16.83 413 794 798 4 812.87 3.16 414 794 799 5 676.94 9.45 415 794 862 68 380.13 16.70 416 795 798 3 815.41 4.62 417 797 855 58 462.18 23.89 418 797 858 61 449.93 17.80 419 797 862 65 380.50 16.57 420 798 855 57 466.01 25.80 421 799 803 4 832.75 11.57 422 799 804 5 799.32 1.63 423 799 807 8 799.32 1.63 424 799 855 56 470.63 28.12 425 799 859 60 461.87 24.12 426 799 862 63 461.86 24.12 427 800 803 3 843.06 10.23 428 800 804 4 838.19 8.88 429 801 804 3 849.46 7.04 430 801 805 4 839.55 8.20 431 802 805 3 849.68 3.14 432 802 806 4 839.57 8.19 433 802 841 39 499.19 14.91 434 803 806 3 855.70 0.12 435 803 807 4 845.62 5.13 436 803 841 38 499.19 14.90 437 803 855 52 471.35 28.82 438 803 859 56 471.03 28.84 439 804 807 3 860.23 5.48 440 804 808 4 841.27 2.18 441 805 808 3 859.50 2.02 442 805 809 4 846.52 0.21 443 806 809 3 852.15 3.03 444 806 810 4 846.41 0.27 445 806 834 28 500.60 14.77 446 806 837 31 500.24 14.62 447 806 838 32 500.24 14.62 448 806 841 35 499.25 14.93 449 807 810 3 846.53 0.21 450 807 811 4 846.51 0.22 451 807 838 31 500.24 14.63 452 807 859 52 471.06 28.89 453 807 862 55 470.95 28.91 454 807 863 56 470.64 28.86 455 808 811 3 845.91 0.10 456 808 812 4 845.90 0.10 457 809 812 3 860.04 6.93 458 809 813 4 845.96 0.12 459 809 834 25 500.67 14.76 460 810 813 3 853.64 3.73 461 810 814 4 842.42 1.83 462 810 831 21 500.88 14.73 463 810 834 24 500.68 14.76 464 810 835 25 500.67 14.76 465 810 838 28 500.56 14.77 466 810 863 53 470.86 28.96 467 810 916 106 383.78 44.97 468 811 814 3 842.38 1.83 469 811 863 52 470.85 28.96 470 811 866 55 467.67 30.46 471 811 867 56 467.61 30.46 472 813 827 14 501.19 14.75 473 813 830 17 501.12 14.73 474 813 831 18 501.04 14.78 475 813 834 21 501.04 14.77 476 813 914 101 385.74 44.77 477 814 831 17 501.12 14.73 478 814 867 53 467.50 30.64 479 814 913 99 385.77 44.80 480 814 914 100 385.70 44.78 481 815 906 91 390.35 43.86 482 815 911 96 388.44 44.51 483 815 912 97 386.75 44.48 484 815 913 98 385.76 44.79 485 815 914 99 385.76 44.79 486 816 821 5 558.43 43.00 487 816 824 8 502.03 14.80 488 816 827 11 501.68 14.95 489 816 911 95 388.86 44.30 490 816 912 96 387.66 44.13 491 816 914 98 386.48 44.59 492 817 820 3 830.64 4.20 493 817 821 4 791.91 13.86 494 817 910 93 389.50 44.08 495 817 911 94 388.86 44.30 496 818 909 91 390.31 43.88 497 818 910 92 390.04 43.87 498 818 912 94 389.36 44.08 499 820 824 4 779.11 11.27 500 821 824 3 815.70 3.23 501 821 827 6 744.93 1.66 502 823 826 3 859.89 0.72 503 823 827 4 852.73 3.57 504 824 827 3 856.91 1.49 505 824 828 4 852.73 3.57 506 825 828 3 864.65 2.39 507 825 829 4 852.74 3.56 508 826 829 3 858.36 0.76 509 826 830 4 840.33 9.76 510 827 830 3 846.50 6.70 511 827 831 4 845.38 7.24 512 827 914 87 387.53 44.12 513 828 831 3 841.59 1.88 514 828 832 4 841.60 1.88 515 829 832 3 841.60 1.88 516 829 833 4 841.58 1.89 517 830 833 3 845.76 0.21 518 830 834 4 840.34 2.50 519 831 834 3 846.65 0.66 520 831 835 4 828.14 3.59 521 831 914 83 385.74 44.77 522 831 916 85 385.69 44.77 523 832 835 3 825.23 6.46 524 832 836 4 835.72 1.22 525 833 836 3 846.32 1.41 526 833 837 4 835.73 1.22 527 834 837 3 835.73 1.22 528 834 838 4 835.71 1.21 529 835 838 3 844.26 3.06 530 835 839 4 830.75 1.32 531 835 856 21 471.28 28.91 532 835 916 81 383.36 45.18 533 836 839 3 843.00 2.43 534 836 840 4 837.98 4.94 535 837 840 3 840.63 3.62 536 837 841 4 832.40 0.49 537 838 841 3 850.97 5.27 538 838 842 4 832.38 0.48 539 838 856 18 471.33 28.89 540 838 859 21 471.18 28.95 541 838 863 25 470.98 28.96 542 839 842 3 846.58 6.58 543 839 843 4 829.40 1.98 544 840 843 3 835.75 1.17 545 840 844 4 821.48 3.19 546 841 845 4 829.32 0.71 547 842 845 3 856.63 2.62 548 842 846 4 835.93 4.01 549 842 852 10 471.59 28.87 550 842 855 13 471.45 28.85 551 842 856 14 471.39 28.88 552 842 859 17 471.37 28.89 553 843 846 3 841.88 4.48 554 843 852 9 471.54 28.89 555 846 851 5 713.04 14.60 556 846 852 6 554.56 33.55 557 846 854 8 686.57 3.47 558 846 855 9 518.06 51.76 559 848 851 3 860.20 0.83 560 848 852 4 834.56 2.21 561 849 852 3 846.56 2.87 562 850 853 3 860.35 4.02 563 850 854 4 825.88 0.70 564 851 854 3 847.08 1.31 565 851 855 4 825.14 0.98 566 852 855 3 841.38 3.38 567 852 856 4 825.53 0.81 568 853 856 3 836.68 1.03 569 853 857 4 825.89 0.62 570 854 857 3 825.94 0.65 571 854 858 4 825.89 0.62 572 855 858 3 830.86 3.11 573 855 859 4 827.41 0.14 574 856 859 3 852.58 0.09 575 856 860 4 820.97 3.36 576 857 860 3 836.65 1.09 577 857 861 4 834.48 2.39 578 857 917 60 382.67 45.48 579 858 861 3 834.66 2.30 580 858 862 4 834.94 2.61 581 859 862 3 834.95 2.62 582 859 863 4 844.80 4.13 583 860 863 3 852.50 0.29 584 860 864 4 844.81 4.13 585 860 916 56 382.70 45.47 586 860 917 57 382.70 45.47 587 861 864 3 852.50 0.29 588 861 865 4 838.36 0.91 589 861 917 56 382.69 45.50 590 862 865 3 848.80 4.72 591 862 866 4 846.07 3.50 592 863 866 3 857.96 2.46 593 863 916 53 382.75 45.48 594 864 867 3 854.27 1.70 595 864 868 4 855.31 1.19 596 864 915 51 382.75 45.49 597 864 916 52 382.94 45.59 598 864 917 53 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