BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 24 94 42 73.45 5.32 2 25 28 3 875.97 2.19 3 25 54 11 81.53 2.56 4 25 57 14 32.70 0.14 5 25 66 15 32.72 0.14 6 25 94 41 82.06 2.65 7 25 98 45 83.42 3.20 8 25 271 212 65.20 5.25 9 27 53 8 82.34 2.64 10 27 54 9 82.32 2.64 11 27 55 10 82.20 2.63 12 27 98 43 83.42 3.20 13 27 102 47 88.64 1.37 14 28 53 7 84.61 2.62 15 28 54 8 82.34 2.64 16 28 271 209 82.32 2.64 17 28 274 212 82.55 2.50 18 28 277 215 83.48 2.06 19 47 50 3 809.00 2.79 20 47 52 5 438.82 94.16 21 47 53 6 85.28 2.48 22 48 52 4 688.15 7.19 23 48 53 5 359.08 134.01 24 48 54 6 359.07 134.02 25 48 209 147 107.14 0.97 26 48 258 194 84.38 2.02 27 48 260 196 83.96 1.83 28 48 269 205 85.14 2.41 29 49 52 3 710.88 2.56 30 49 207 144 103.14 1.25 31 49 208 145 105.43 0.12 32 49 209 146 106.61 0.71 33 49 214 151 98.58 1.24 34 49 216 153 88.50 1.26 35 50 207 143 109.74 0.74 36 50 208 144 108.61 0.24 37 50 209 145 110.23 0.41 38 50 210 146 110.35 0.45 39 50 211 147 110.36 0.45 40 51 73 14 110.69 0.57 41 51 74 15 110.69 0.57 42 51 207 142 110.39 0.48 43 51 208 143 110.62 0.56 44 52 72 12 110.98 0.51 45 52 73 13 110.83 0.55 46 52 205 139 110.67 0.57 47 52 207 141 110.64 0.56 48 52 216 150 110.62 0.58 49 53 71 10 111.04 0.51 50 53 72 11 111.05 0.51 51 53 74 13 111.73 0.84 52 53 102 39 125.58 0.78 53 54 57 3 898.21 2.48 54 54 68 6 670.34 1.03 55 54 69 7 368.10 128.02 56 54 70 8 111.06 0.51 57 54 71 9 111.07 0.51 58 54 271 201 115.95 0.07 59 55 70 7 370.36 126.89 60 55 72 9 117.41 0.29 61 55 82 18 117.41 0.29 62 55 94 29 164.34 1.33 63 55 95 30 135.15 1.27 64 55 98 33 135.18 1.25 65 55 99 34 133.17 0.24 66 56 68 4 681.27 4.43 67 56 70 6 371.06 126.54 68 56 91 25 165.18 1.25 69 56 94 28 165.50 1.16 70 56 95 29 141.58 1.23 71 57 68 3 724.23 2.85 72 57 91 24 165.58 1.19 73 57 94 27 165.77 1.17 74 57 271 198 141.68 0.62 75 66 264 190 160.16 0.47 76 66 271 197 141.68 0.62 77 66 272 198 148.05 1.03 78 67 264 189 162.15 0.29 79 68 86 17 165.56 1.19 80 68 87 18 165.63 1.18 81 68 91 21 167.28 0.91 82 68 120 50 165.55 1.18 83 68 121 51 165.52 1.19 84 68 262 186 162.54 0.44 85 68 263 187 162.62 0.47 86 68 264 188 162.65 0.46 87 69 84 14 165.64 1.18 88 69 218 143 162.71 0.52 89 69 261 184 162.62 0.48 90 69 262 185 162.62 0.48 91 69 264 187 162.66 0.46 92 69 271 194 162.57 0.52 93 70 83 12 165.62 1.17 94 70 84 13 165.64 1.18 95 70 85 14 165.66 1.17 96 70 95 23 167.56 0.93 97 71 82 10 166.24 0.92 98 71 83 11 165.66 1.18 99 71 205 128 165.71 1.15 100 71 218 141 165.28 1.20 101 71 260 181 162.82 0.53 102 72 80 7 394.78 114.55 103 72 81 8 394.54 114.59 104 72 82 9 166.63 0.94 105 72 99 25 286.08 60.09 106 72 102 28 373.20 78.59 107 72 104 30 369.92 78.35 108 72 109 35 304.84 69.57 109 73 80 6 394.79 114.54 110 73 81 7 394.62 114.57 111 73 83 9 394.57 114.58 112 73 187 109 200.18 17.70 113 73 205 126 200.18 17.71 114 74 80 5 394.79 114.54 115 74 102 26 375.87 77.55 116 74 104 28 375.37 77.67 117 75 79 3 735.00 1.62 118 75 81 5 518.59 57.30 119 75 182 102 256.62 45.48 120 75 183 103 256.68 45.49 121 75 185 105 256.62 45.48 122 76 183 102 269.47 39.65 123 78 183 101 278.83 44.28 124 79 108 28 375.76 77.56 125 79 182 99 289.88 42.40 126 80 104 23 376.29 77.55 127 80 108 27 376.11 77.56 128 80 109 28 375.81 77.64 129 80 110 29 375.88 77.67 130 80 111 30 375.88 77.67 131 81 109 27 376.19 77.59 132 81 111 29 375.91 77.69 133 81 113 31 376.26 77.64 134 81 182 97 295.35 41.27 135 81 187 102 293.86 41.73 136 82 95 12 434.23 93.08 137 82 99 16 433.81 93.28 138 82 109 26 376.42 77.61 139 82 111 28 376.26 77.64 140 82 112 29 376.07 77.72 141 82 113 30 376.26 77.64 142 82 128 45 376.42 77.62 143 83 113 29 376.25 77.64 144 83 187 100 295.36 41.27 145 83 203 115 262.67 28.54 146 83 205 117 262.72 28.50 147 83 218 130 256.81 25.85 148 84 113 28 407.91 93.37 149 84 114 29 410.21 94.55 150 84 115 30 417.07 97.97 151 84 121 36 420.94 99.89 152 84 218 129 257.49 25.91 153 85 91 5 663.97 9.53 154 85 95 9 434.49 93.13 155 85 121 35 434.44 93.17 156 85 124 38 416.03 84.64 157 86 91 4 720.04 2.44 158 86 119 32 434.56 93.14 159 86 120 33 434.53 93.13 160 86 121 34 434.44 93.17 161 86 122 35 434.45 93.16 162 86 123 36 434.45 93.14 163 86 124 37 434.48 93.11 164 87 91 3 734.97 1.41 165 87 119 31 434.56 93.14 166 87 120 32 434.54 93.13 167 87 123 35 434.51 93.11 168 87 124 36 434.53 93.11 169 89 119 30 434.59 93.14 170 89 123 34 434.51 93.11 171 89 124 35 434.51 93.10 172 89 164 73 344.42 48.04 173 89 165 74 344.42 48.04 174 91 94 3 741.31 2.08 175 91 95 4 726.02 0.42 176 91 124 33 434.61 93.06 177 92 95 3 755.40 0.77 178 92 96 4 726.00 0.42 179 92 124 32 434.61 93.05 180 92 127 35 434.60 92.97 181 92 128 36 434.09 92.75 182 92 131 39 433.76 92.31 183 93 96 3 743.97 2.62 184 93 97 4 726.25 0.54 185 93 131 38 434.02 92.19 186 94 97 3 732.78 2.98 187 94 98 4 730.85 1.80 188 95 98 3 749.37 2.33 189 95 99 4 733.48 4.19 190 95 124 29 435.01 92.88 191 95 128 33 434.58 92.53 192 96 99 3 746.71 3.70 193 96 100 4 733.48 4.19 194 96 106 10 436.20 93.25 195 96 128 32 434.45 92.59 196 96 131 35 434.26 92.08 197 96 132 36 434.05 92.17 198 97 100 3 751.58 1.27 199 97 101 4 740.52 0.67 200 98 101 3 732.20 4.83 201 98 102 4 731.85 4.65 202 99 102 3 747.62 3.23 203 99 103 4 731.46 4.85 204 99 104 5 695.38 13.10 205 99 109 10 437.14 93.75 206 100 103 3 734.12 4.05 207 100 104 4 715.11 3.31 208 100 105 5 697.05 10.43 209 104 108 4 709.56 3.48 210 104 109 5 686.41 8.09 211 105 108 3 735.74 2.39 212 105 109 4 730.84 4.41 213 106 109 3 745.61 2.37 214 106 131 25 468.18 75.71 215 106 132 26 461.20 78.61 216 109 112 3 865.60 0.74 217 109 132 23 469.45 74.47 218 109 133 24 469.45 74.47 219 109 134 25 469.19 74.34 220 110 134 24 469.18 74.33 221 110 135 25 469.17 74.33 222 110 182 69 342.33 17.79 223 111 134 23 469.18 74.33 224 111 135 24 469.14 74.32 225 111 137 26 456.00 67.75 226 111 180 66 345.24 16.47 227 111 182 68 345.10 16.40 228 112 125 13 470.76 74.99 229 112 128 16 470.80 74.95 230 112 134 22 469.29 74.27 231 112 135 23 469.10 74.29 232 112 136 24 455.94 67.72 233 112 137 25 455.57 67.53 234 113 137 24 445.03 62.26 235 113 180 64 345.44 16.43 236 113 187 71 345.19 16.37 237 113 203 86 313.23 3.25 238 114 121 7 471.78 75.14 239 114 122 8 471.60 75.22 240 114 125 11 471.30 75.09 241 114 136 22 455.73 67.46 242 114 146 32 396.97 38.06 243 114 201 83 328.77 8.19 244 114 203 85 314.04 3.20 245 115 200 81 332.21 6.81 246 115 201 82 327.99 7.80 247 115 218 99 308.40 0.46 248 115 220 101 307.52 0.03 249 115 262 141 257.66 24.54 250 116 121 5 472.61 75.25 251 116 122 6 472.13 75.02 252 116 196 76 333.66 6.92 253 116 197 77 333.67 6.80 254 116 200 80 332.79 6.55 255 116 220 100 312.62 1.85 256 116 221 101 313.69 2.56 257 117 122 5 472.14 75.02 258 117 142 25 417.20 48.22 259 117 145 28 397.87 38.50 260 117 146 29 396.99 38.06 261 117 164 45 381.78 29.84 262 118 121 3 743.08 1.32 263 118 122 4 663.11 6.30 264 118 145 27 416.32 47.61 265 118 153 35 381.68 30.23 266 118 156 38 382.06 30.01 267 118 164 44 381.76 29.84 268 118 196 74 333.87 6.87 269 118 197 75 333.79 6.83 270 118 228 105 306.30 7.15 271 119 123 4 472.35 75.12 272 119 162 41 381.74 29.82 273 119 164 43 381.70 29.81 274 121 262 135 257.67 24.55 275 121 263 136 257.67 24.55 276 122 125 3 748.27 1.61 277 122 146 24 397.01 38.04 278 122 164 40 381.64 29.50 279 123 164 39 381.55 29.47 280 124 127 3 748.55 0.93 281 124 128 4 731.00 4.83 282 125 128 3 744.61 1.04 283 125 129 4 742.46 0.89 284 125 136 11 440.42 59.09 285 125 146 21 397.59 37.47 286 125 168 40 379.49 28.39 287 126 129 3 745.47 0.61 288 126 130 4 742.46 0.89 289 126 165 37 379.68 28.41 290 126 166 38 379.57 28.39 291 126 168 39 379.48 28.38 292 127 130 3 742.46 0.89 293 127 131 4 728.23 7.99 294 128 131 3 741.28 1.48 295 128 132 4 716.40 2.08 296 129 132 3 741.36 1.42 297 129 136 7 456.92 67.04 298 129 168 36 381.83 27.20 299 129 169 37 381.83 27.20 300 129 171 39 380.82 26.70 301 135 171 33 413.59 9.69 302 136 146 10 643.72 10.09 303 136 170 31 413.62 9.70 304 136 171 32 413.61 9.70 305 136 172 33 413.61 9.70 306 137 172 32 413.58 9.69 307 137 174 34 413.39 9.59 308 137 180 40 382.61 1.96 309 138 142 4 711.70 2.21 310 138 143 5 696.99 4.53 311 138 170 29 414.04 9.85 312 138 172 31 413.74 9.73 313 138 173 32 413.56 9.67 314 138 174 33 413.39 9.59 315 139 142 3 735.40 2.05 316 139 143 4 718.70 0.91 317 139 173 31 413.64 9.69 318 139 174 32 413.41 9.60 319 139 191 49 385.79 3.98 320 139 199 56 370.36 11.71 321 139 201 58 370.05 11.65 322 140 143 3 734.32 4.53 323 140 144 4 720.00 0.26 324 140 173 30 413.76 9.73 325 141 144 3 745.17 7.27 326 141 145 4 719.96 1.51 327 141 198 53 370.66 11.70 328 141 199 54 370.48 11.71 329 142 145 3 733.02 4.75 330 142 146 4 719.95 1.52 331 142 199 53 370.37 11.66 332 143 146 3 735.57 1.41 333 143 147 4 720.03 1.47 334 143 170 24 415.16 10.37 335 143 173 27 414.03 9.85 336 144 147 3 745.37 0.36 337 144 148 4 720.03 1.47 338 145 148 3 748.89 1.42 339 145 149 4 726.12 3.31 340 145 150 5 697.34 4.57 341 145 151 6 697.34 4.57 342 146 149 3 740.03 3.01 343 146 164 16 419.64 10.91 344 146 166 18 416.45 9.94 345 146 168 19 416.44 9.95 346 146 170 21 416.30 9.96 347 147 166 17 416.45 9.95 348 147 168 18 416.43 9.96 349 148 151 3 762.30 2.81 350 148 152 4 762.30 2.81 351 148 166 16 418.62 11.03 352 149 163 12 419.68 10.92 353 149 164 13 419.62 10.90 354 149 166 15 418.28 10.86 355 150 153 3 759.19 0.24 356 150 164 12 419.68 10.92 357 151 157 6 420.50 10.98 358 151 160 7 420.34 10.98 359 151 163 10 419.69 10.92 360 152 157 5 420.47 11.00 361 152 160 6 420.45 11.00 362 152 235 78 307.30 45.26 363 153 156 3 718.73 0.96 364 153 157 4 420.50 10.98 365 154 198 40 372.62 12.48 366 154 235 76 307.27 45.24 367 154 236 77 307.25 45.26 368 155 228 68 344.37 25.71 369 155 233 73 330.00 33.93 370 155 236 76 307.31 45.27 371 155 239 79 307.24 45.27 372 156 161 3 752.84 9.06 373 156 162 4 724.11 5.29 374 156 226 65 351.32 22.21 375 156 228 67 347.25 24.82 376 170 173 3 787.96 1.77 377 172 178 6 706.79 1.16 378 172 180 8 423.97 9.84 379 174 178 4 689.46 2.16 380 174 180 6 666.02 10.08 381 174 191 17 390.19 6.04 382 175 178 3 732.61 0.58 383 175 191 16 390.21 6.03 384 176 189 13 391.14 6.12 385 176 190 14 390.52 5.91 386 176 191 15 390.20 6.04 387 177 188 11 391.32 6.11 388 177 189 12 391.14 6.12 389 178 188 10 391.34 6.12 390 178 189 11 391.29 6.11 391 179 186 7 392.20 6.27 392 179 187 8 391.99 6.32 393 179 188 9 391.31 6.13 394 180 186 6 392.20 6.27 395 180 187 7 392.18 6.27 396 180 203 22 374.16 15.28 397 181 184 3 760.23 0.21 398 181 185 4 636.62 1.04 399 181 186 5 392.22 6.27 400 182 185 3 729.83 5.19 401 182 187 5 666.50 12.04 402 185 205 19 420.03 54.63 403 186 205 18 419.91 54.98 404 187 203 15 432.12 49.05 405 187 204 16 429.34 50.27 406 187 205 17 419.84 55.02 407 188 203 14 438.67 47.54 408 188 204 15 431.89 49.08 409 189 201 11 447.53 50.81 410 189 202 12 441.45 47.95 411 189 203 13 440.87 47.71 412 190 201 10 447.88 50.97 413 190 202 11 441.46 47.95 414 190 204 13 441.47 47.98 415 190 249 57 379.11 85.22 416 190 254 61 371.47 81.56 417 191 200 8 448.68 50.77 418 191 201 9 448.29 50.79 419 191 249 56 379.43 85.38 420 192 200 7 448.72 50.76 421 192 201 8 448.68 50.77 422 192 202 9 448.57 50.81 423 192 219 26 429.96 60.12 424 192 220 27 429.93 60.14 425 192 221 28 429.88 60.19 426 192 222 29 429.70 60.28 427 192 246 52 379.58 85.31 428 192 250 56 379.45 85.37 429 194 197 3 756.42 1.82 430 194 198 4 516.53 21.34 431 194 200 6 448.77 50.76 432 194 236 41 387.99 85.66 433 194 242 47 380.34 85.03 434 194 243 48 380.35 85.03 435 194 246 51 379.68 85.31 436 195 198 3 740.71 3.23 437 195 228 32 423.54 67.67 438 195 236 40 388.01 85.67 439 195 243 47 380.34 85.03 440 196 200 4 709.75 6.73 441 196 221 25 429.88 60.29 442 196 223 27 429.95 60.72 443 196 226 29 433.11 62.88 444 196 228 31 421.48 68.70 445 196 243 46 384.61 87.17 446 197 200 3 729.27 3.04 447 198 236 37 388.02 85.67 448 200 220 20 434.10 62.42 449 201 220 19 434.07 62.46 450 202 218 16 434.25 62.38 451 202 219 17 434.02 62.48 452 202 254 50 383.81 87.62 453 203 217 14 434.37 62.34 454 203 218 15 434.26 62.39 455 204 216 12 434.72 62.51 456 204 217 13 434.36 62.36 457 204 254 48 383.87 87.77 458 205 215 10 434.88 62.48 459 205 216 11 434.77 62.54 460 205 218 13 434.74 62.55 461 206 213 7 435.65 62.50 462 206 214 8 435.50 62.54 463 206 215 9 434.89 62.48 464 206 216 10 434.88 62.49 465 207 213 6 435.66 62.50 466 207 214 7 435.63 62.50 467 207 216 9 435.29 62.67 468 208 211 3 713.59 8.84 469 208 212 4 527.40 16.64 470 208 213 5 435.67 62.50 471 208 214 6 435.66 62.50 472 209 212 3 746.05 7.08 473 209 214 5 435.68 62.50 474 214 257 41 415.62 103.70 475 214 258 42 415.79 103.70 476 215 255 38 415.36 103.56 477 215 256 39 415.25 103.57 478 215 257 40 415.68 103.73 479 215 258 41 416.25 103.89 480 216 258 40 416.21 103.92 481 216 260 42 418.33 104.81 482 217 254 35 416.29 103.89 483 217 256 37 414.87 104.59 484 217 258 39 418.23 104.86 485 217 259 40 417.31 104.41 486 217 260 41 418.33 104.81 487 217 290 65 409.95 107.06 488 218 260 40 418.33 104.81 489 218 261 41 418.42 104.84 490 218 262 42 418.47 104.86 491 219 250 30 417.38 104.36 492 219 254 33 418.31 104.88 493 219 259 38 418.43 104.84 494 219 260 39 418.43 104.84 495 219 261 40 418.43 104.84 496 219 262 41 418.48 104.86 497 220 262 40 418.48 104.86 498 220 293 65 410.03 109.03 499 221 262 39 418.50 104.86 500 221 293 64 410.44 108.84 501 222 243 20 419.22 104.47 502 222 246 23 418.97 104.60 503 222 247 24 418.92 104.62 504 222 250 27 418.63 104.75 505 222 293 63 410.44 108.84 506 222 294 64 409.98 109.04 507 222 297 67 365.42 130.94 508 223 243 19 419.25 104.48 509 224 228 3 714.49 0.58 510 224 230 5 706.81 3.26 511 224 243 18 419.27 104.47 512 224 304 72 334.43 139.50 513 228 239 11 423.11 103.20 514 228 243 15 422.67 103.19 515 229 232 3 815.60 3.46 516 229 233 4 700.15 5.35 517 229 234 5 741.97 0.35 518 229 239 10 423.12 103.20 519 230 233 3 773.64 2.41 520 230 239 9 423.12 103.20 521 230 240 10 423.09 103.20 522 230 301 64 366.30 130.56 523 230 304 67 352.88 134.05 524 230 305 68 334.81 139.69 525 230 308 71 321.12 136.31 526 231 240 9 423.12 103.20 527 231 305 67 349.49 132.41 528 231 308 70 325.92 136.09 529 231 309 71 319.31 135.41 530 233 236 3 724.18 1.44 531 233 237 4 657.38 12.39 532 234 237 3 731.89 1.86 533 236 239 3 740.28 1.22 534 236 242 6 721.28 1.74 535 238 241 3 763.96 4.68 536 238 242 4 742.04 0.35 537 239 242 3 745.09 1.16 538 239 243 4 742.06 0.36 539 239 301 55 366.47 130.62 540 240 243 3 745.09 1.16 541 240 244 4 742.05 0.36 542 240 301 54 366.46 130.61 543 240 305 58 334.80 139.68 544 241 244 3 745.07 1.17 545 241 245 4 736.93 0.49 546 242 245 3 740.36 1.22 547 242 246 4 736.81 0.56 548 243 246 3 736.81 0.56 549 243 247 4 739.29 1.75 550 243 301 51 364.00 131.77 551 244 247 3 739.33 1.74 552 244 248 4 739.33 1.75 553 244 298 47 401.62 113.34 554 244 301 50 365.35 131.10 555 244 302 51 363.94 131.78 556 245 248 3 748.59 2.89 557 245 249 4 739.32 1.74 558 246 249 3 739.32 1.74 559 246 250 4 739.32 1.74 560 247 250 3 744.74 1.07 561 247 251 4 732.88 1.50 562 247 294 40 408.41 110.09 563 247 297 43 406.95 110.81 564 247 298 44 376.70 125.92 565 247 301 47 363.81 131.84 566 248 251 3 744.73 1.08 567 249 252 3 748.71 0.46 568 249 254 4 730.31 2.79 569 250 254 3 746.04 0.42 570 250 290 33 413.49 108.59 571 250 293 36 411.35 109.64 572 250 294 37 411.19 109.70 573 251 290 32 416.90 106.89 574 251 291 33 413.00 108.84 575 251 294 36 411.25 109.69 576 251 295 37 411.22 109.70 577 251 298 40 405.58 112.52 578 254 290 30 732.78 1.67 579 289 293 4 897.00 3.98 580 290 293 3 753.06 0.70 581 290 294 4 724.16 3.84 582 291 294 3 750.21 1.83 583 291 295 4 724.17 3.83 584 292 295 3 740.74 2.71 585 292 296 4 721.48 2.50 586 293 296 3 729.11 1.30 587 293 297 4 723.40 6.32 588 294 297 3 737.62 0.79 589 294 298 4 730.04 3.00 590 295 298 3 744.56 1.90 591 295 299 4 723.35 6.34 592 296 299 3 739.08 4.65 593 296 300 4 723.17 6.44 594 297 300 3 731.64 0.93 595 297 301 4 715.08 2.71 596 298 301 3 711.01 0.67 597 298 302 4 710.73 0.53 598 299 302 3 732.76 0.29 599 299 303 4 714.71 2.52 600 300 303 3 731.23 3.08 601 300 304 4 723.54 4.04 602 301 304 3 735.37 1.00 603 301 305 4 720.64 5.49 604 302 306 4 721.39 5.26 605 303 306 3 730.37 2.45 606 303 307 4 733.95 1.01 607 304 307 3 736.87 1.88 608 304 308 4 735.86 1.96 609 305 308 3 735.86 1.97 610 305 309 4 735.84 1.97 611 306 309 3 740.52 0.01 612 306 310 4 730.03 4.87 613 307 310 3 759.55 4.03 614 307 311 4 587.70 23.27