BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 671 840 169 22.77 0.52 2 672 675 3 848.71 3.08 3 672 676 4 830.47 1.77 4 673 676 3 858.45 0.93 5 673 677 4 832.26 0.70 6 673 806 133 24.76 0.07 7 673 834 161 24.29 0.06 8 673 837 164 24.64 0.05 9 673 840 167 26.66 1.02 10 674 677 3 845.22 4.59 11 674 678 4 825.74 3.97 12 674 802 128 26.77 0.96 13 674 841 167 26.69 1.01 14 675 678 3 849.23 4.11 15 675 679 4 824.53 3.73 16 676 679 3 830.68 4.50 17 676 680 4 830.68 4.50 18 676 721 45 33.20 1.02 19 676 723 47 30.07 0.24 20 677 680 3 838.27 0.82 21 677 681 4 817.78 1.68 22 677 722 45 33.16 0.99 23 677 805 128 29.55 0.07 24 677 806 129 27.14 1.14 25 677 809 132 27.14 1.14 26 678 681 3 841.62 6.87 27 678 805 127 30.23 0.40 28 680 718 38 33.39 1.05 29 680 721 41 33.42 1.05 30 680 722 42 33.38 1.05 31 681 718 37 33.85 0.86 32 681 804 123 33.29 1.06 33 681 805 124 33.22 1.02 34 681 808 127 33.29 1.06 35 682 714 32 36.59 0.70 36 682 718 36 33.88 0.86 37 684 766 82 82.92 0.67 38 685 707 22 82.94 0.65 39 685 710 25 82.77 0.57 40 685 711 26 77.47 0.51 41 685 714 29 77.17 0.69 42 685 765 80 82.78 0.65 43 685 766 81 82.94 0.66 44 686 707 21 82.95 0.65 45 686 710 24 83.00 0.62 46 686 765 79 82.91 0.66 47 686 766 80 82.94 0.66 48 686 767 81 83.05 0.70 49 687 703 16 83.06 0.60 50 687 706 19 83.06 0.61 51 687 707 20 82.95 0.65 52 687 710 23 83.12 0.68 53 687 765 78 82.98 0.67 54 687 767 80 83.38 0.61 55 687 768 81 83.22 0.69 56 688 710 22 83.38 0.61 57 688 767 79 83.69 0.65 58 689 699 10 83.73 0.65 59 689 700 11 83.73 0.65 60 689 703 14 83.57 0.62 61 689 767 78 83.70 0.66 62 689 768 79 83.70 0.66 63 689 774 85 83.75 0.64 64 690 693 3 870.14 3.64 65 691 767 76 83.78 0.66 66 691 774 83 99.78 2.73 67 695 700 5 802.48 8.80 68 695 773 78 130.61 0.49 69 695 774 79 129.65 0.93 70 695 777 82 130.65 0.48 71 696 699 3 844.16 4.71 72 696 700 4 824.18 5.28 73 696 777 81 130.78 0.43 74 697 700 3 844.18 4.71 75 697 701 4 823.18 4.76 76 697 777 80 130.78 0.43 77 697 778 81 130.78 0.43 78 697 779 82 130.78 0.43 79 697 876 179 131.34 0.15 80 697 880 183 144.62 1.25 81 697 887 190 146.28 1.18 82 697 889 192 146.30 1.15 83 698 701 3 855.31 5.30 84 698 702 4 823.19 4.75 85 698 887 189 146.28 1.18 86 698 888 190 146.33 1.15 87 698 889 191 146.23 1.19 88 699 702 3 833.88 0.42 89 699 703 4 832.86 0.07 90 700 703 3 833.51 0.45 91 700 704 4 825.32 4.54 92 700 770 70 146.34 1.14 93 700 774 74 146.34 1.14 94 700 777 77 146.77 0.95 95 700 778 78 146.38 1.14 96 701 704 3 830.37 2.02 97 701 705 4 824.00 5.20 98 701 778 77 146.78 0.95 99 701 780 79 146.78 0.96 100 701 876 175 146.38 1.15 101 701 880 179 146.75 0.96 102 701 889 188 148.71 0.34 103 702 705 3 831.92 1.34 104 702 706 4 830.87 0.83 105 702 889 187 148.71 0.33 106 702 890 188 148.71 0.34 107 703 706 3 838.44 1.94 108 703 707 4 830.87 0.83 109 703 768 65 151.06 0.42 110 704 707 3 843.12 6.95 111 704 708 4 826.01 1.61 112 704 764 60 149.36 0.42 113 704 768 64 153.02 0.55 114 704 770 66 153.00 0.55 115 705 708 3 828.72 0.25 116 705 709 4 828.75 0.27 117 705 889 184 159.34 0.90 118 705 890 185 159.38 0.88 119 705 891 186 159.25 0.86 120 705 892 187 159.40 0.88 121 705 895 190 156.33 0.29 122 706 709 3 838.80 1.75 123 706 710 4 829.55 5.03 124 706 892 186 161.63 0.39 125 707 710 3 836.42 1.60 126 707 711 4 836.41 1.59 127 707 764 57 157.23 0.07 128 707 765 58 159.78 1.31 129 707 768 61 159.79 1.31 130 708 711 3 836.42 1.60 131 708 712 4 831.71 0.76 132 708 745 37 157.24 0.09 133 708 895 187 164.59 0.67 134 709 712 3 841.24 4.79 135 709 713 4 832.46 0.41 136 709 892 183 167.56 0.70 137 709 894 185 167.65 0.65 138 709 895 186 166.01 0.01 139 710 713 3 847.48 3.02 140 710 714 4 832.49 0.42 141 711 714 3 839.38 1.02 142 711 715 4 831.78 0.77 143 711 745 34 167.49 0.82 144 712 715 3 831.77 0.78 145 712 716 4 833.63 0.26 146 712 737 25 170.98 0.15 147 712 741 29 168.56 1.05 148 712 745 33 168.59 1.06 149 712 894 182 171.81 0.46 150 712 895 183 171.70 0.47 151 712 898 186 171.71 0.47 152 713 716 3 835.28 0.93 153 713 717 4 833.81 0.19 154 714 717 3 835.28 0.93 155 714 718 4 833.80 0.19 156 715 718 3 833.80 0.19 157 715 719 4 833.80 0.19 158 715 737 22 174.85 1.05 159 715 744 29 171.78 0.45 160 715 745 30 171.80 0.46 161 716 719 3 833.81 0.19 162 716 720 4 833.80 0.19 163 716 737 21 175.44 0.78 164 717 720 3 838.62 2.62 165 717 721 4 829.01 2.18 166 718 721 3 833.81 2.10 167 718 722 4 829.01 2.18 168 718 744 26 178.80 1.96 169 718 808 90 178.80 1.96 170 718 809 91 178.80 1.96 171 718 812 94 178.84 1.93 172 719 722 3 852.51 4.90 173 719 725 6 630.92 10.22 174 719 737 18 178.84 1.92 175 719 812 93 178.76 1.96 176 720 725 5 624.54 5.73 177 720 726 6 536.28 0.16 178 722 725 3 820.61 4.87 179 722 809 87 179.27 1.78 180 722 812 90 179.38 1.78 181 722 834 112 179.41 1.77 182 723 830 107 179.53 1.74 183 723 834 111 179.44 1.76 184 724 727 3 836.38 2.55 185 724 830 106 179.53 1.74 186 725 728 3 859.73 3.04 187 725 733 8 574.96 17.11 188 725 736 11 548.21 3.80 189 725 737 12 358.61 91.06 190 725 812 87 179.80 1.70 191 725 813 88 179.80 1.70 192 725 830 105 179.80 1.70 193 726 733 7 647.63 3.50 194 727 826 99 180.24 1.84 195 728 732 4 812.63 1.83 196 728 733 5 789.68 3.62 197 728 813 85 180.28 1.86 198 728 827 99 180.25 1.84 199 728 830 102 180.25 1.84 200 729 732 3 825.48 2.42 201 729 733 4 825.01 2.17 202 729 822 93 221.54 0.52 203 730 733 3 830.23 0.55 204 730 734 4 825.86 2.58 205 731 734 3 847.38 1.52 206 731 735 4 825.87 2.59 207 731 822 91 229.71 1.41 208 732 735 3 836.28 2.64 209 732 736 4 825.88 2.60 210 732 821 89 229.66 1.40 211 732 822 90 229.71 1.41 212 732 823 91 229.85 1.34 213 733 736 3 860.72 0.34 214 733 737 4 825.75 2.70 215 734 737 3 838.59 2.19 216 734 738 4 825.75 2.70 217 735 738 3 828.16 1.50 218 735 739 4 822.60 4.28 219 735 821 86 251.79 3.04 220 736 739 3 837.53 3.19 221 736 740 4 817.29 1.63 222 736 812 76 238.00 0.18 223 736 816 80 238.00 0.18 224 736 821 85 258.31 0.43 225 736 827 91 238.06 0.15 226 737 741 4 801.90 2.42 227 737 812 75 242.49 2.06 228 738 741 3 854.90 4.22 229 738 898 160 273.73 1.20 230 738 902 164 268.56 1.24 231 739 815 76 259.41 0.17 232 739 817 78 268.51 1.24 233 739 902 163 271.82 2.10 234 739 905 166 271.05 2.48 235 739 906 167 262.69 1.69 236 739 911 172 262.65 1.66 237 740 743 3 842.97 2.67 238 740 744 4 824.80 2.32 239 740 811 71 259.94 0.37 240 740 812 72 259.94 0.37 241 740 815 75 260.00 0.34 242 741 744 3 852.32 2.69 243 741 745 4 828.16 3.16 244 741 895 154 284.13 0.42 245 741 898 157 282.87 0.97 246 741 899 158 282.86 0.67 247 741 903 162 277.10 1.04 248 742 745 3 852.32 2.69 249 742 746 4 838.16 4.37 250 742 870 128 277.06 1.03 251 742 873 131 277.24 0.96 252 742 874 132 277.12 1.03 253 742 877 135 283.50 0.70 254 743 746 3 854.97 4.03 255 743 747 4 828.19 3.16 256 743 811 68 276.99 1.05 257 743 866 123 276.99 1.05 258 743 870 127 277.08 1.03 259 744 747 3 848.75 7.13 260 744 748 4 823.48 5.50 261 744 808 64 277.06 1.03 262 744 811 67 277.06 1.03 263 744 812 68 277.04 1.04 264 745 748 3 855.31 4.45 265 745 749 4 823.25 5.39 266 746 749 3 826.22 3.92 267 746 750 4 834.63 0.29 268 746 787 41 286.07 0.42 269 746 790 44 286.26 0.34 270 746 869 123 285.72 0.55 271 746 873 127 285.72 0.55 272 747 750 3 834.73 0.24 273 747 751 4 834.65 0.29 274 747 790 43 286.27 0.34 275 747 794 47 286.31 0.33 276 747 804 57 286.31 0.33 277 747 807 60 286.25 0.32 278 747 808 61 286.23 0.32 279 747 811 64 285.93 0.47 280 747 862 115 285.88 0.48 281 747 866 119 285.72 0.55 282 748 751 3 833.06 1.10 283 748 752 4 839.92 1.92 284 748 804 56 286.31 0.30 285 748 808 60 286.23 0.33 286 749 752 3 847.50 1.88 287 749 753 4 839.92 1.91 288 749 762 13 289.33 0.52 289 749 763 14 289.35 0.53 290 749 764 15 289.28 0.48 291 749 787 38 286.74 0.15 292 750 753 3 839.15 1.95 293 750 754 4 835.68 0.21 294 750 791 41 286.69 0.14 295 750 794 44 286.94 0.24 296 751 754 3 840.50 2.19 297 751 755 4 831.53 1.75 298 751 794 43 287.10 0.21 299 751 799 48 287.00 0.26 300 751 801 50 287.02 0.25 301 751 804 53 286.75 0.14 302 752 755 3 840.38 2.25 303 752 756 4 831.45 1.79 304 752 763 11 289.62 0.40 305 752 764 12 289.67 0.40 306 753 757 4 751.06 6.28 307 753 758 5 657.73 3.83 308 753 759 6 384.85 47.19 309 753 761 8 289.87 0.29 310 753 762 9 289.63 0.40 311 753 763 10 289.63 0.40 312 753 791 38 287.74 0.48 313 754 758 4 611.60 0.55 314 754 759 5 403.53 56.52 315 754 791 37 287.75 0.48 316 754 794 40 287.49 0.35 317 754 795 41 287.78 0.49 318 756 763 7 290.02 0.36 319 757 760 3 847.62 1.17 320 757 761 4 753.07 6.36 321 757 763 6 391.99 50.53 322 759 788 29 288.45 0.35 323 759 791 32 288.41 0.35 324 759 792 33 288.35 0.33 325 761 769 8 290.32 0.58 326 761 770 9 290.27 0.59 327 761 771 10 290.13 0.62 328 761 775 14 290.25 0.60 329 762 769 7 290.32 0.58 330 762 770 8 290.26 0.60 331 762 775 13 290.25 0.60 332 762 784 22 288.49 0.34 333 762 787 25 288.47 0.34 334 762 788 26 288.45 0.34 335 763 766 3 896.88 0.90 336 763 769 6 290.32 0.58 337 764 768 4 792.83 13.74 338 764 770 6 571.28 20.96 339 764 787 23 288.46 0.34 340 765 768 3 827.50 8.04 341 765 769 4 793.03 13.64 342 768 774 6 802.03 2.54 343 769 774 5 815.51 0.12 344 770 774 4 811.45 2.16 345 770 775 5 802.03 2.54 346 770 778 8 742.84 14.69 347 771 774 3 832.50 1.53 348 771 775 4 828.68 3.43 349 772 775 3 835.80 0.12 350 772 776 4 828.69 3.43 351 772 781 9 607.59 2.85 352 772 788 16 297.55 3.62 353 773 776 3 853.66 1.91 354 773 777 4 828.69 3.43 355 774 777 3 832.93 1.29 356 774 778 4 825.65 4.92 357 775 778 3 844.51 0.68 358 775 780 5 758.51 6.24 359 775 781 6 607.59 2.85 360 775 784 9 550.59 9.46 361 775 788 13 543.01 5.67 362 776 779 3 850.16 3.50 363 776 781 5 669.84 33.98 364 778 876 98 414.25 0.07 365 779 876 97 414.26 0.07 366 779 887 108 416.32 0.73 367 780 783 3 856.19 0.51 368 780 784 4 829.98 3.32 369 780 787 7 829.98 3.33 370 780 873 93 414.26 0.07 371 780 876 96 414.29 0.08 372 781 784 3 859.39 6.36 373 781 785 4 830.08 3.29 374 782 785 3 854.69 0.24 375 782 786 4 845.44 4.38 376 783 786 3 851.62 2.19 377 783 787 4 841.67 2.50 378 783 872 89 415.92 0.76 379 783 873 90 416.01 0.73 380 783 876 93 416.09 0.70 381 784 787 3 839.96 1.64 382 784 788 4 830.09 3.29 383 785 788 3 839.96 1.64 384 785 789 4 830.00 3.27 385 786 789 3 827.45 3.13 386 786 790 4 822.32 0.57 387 786 868 82 416.21 0.77 388 786 869 83 416.21 0.77 389 786 872 86 416.49 0.76 390 787 790 3 826.64 5.17 391 787 791 4 818.35 1.36 392 787 869 82 416.34 0.83 393 787 873 86 416.80 0.68 394 788 791 3 829.89 3.54 395 788 792 4 818.34 1.36 396 789 792 3 845.38 1.26 397 789 793 4 815.54 0.46 398 789 796 7 792.28 0.44 399 790 793 3 838.43 4.74 400 790 794 4 835.82 4.74 401 790 862 72 416.57 0.76 402 790 865 75 416.68 0.72 403 790 866 76 416.80 0.67 404 790 869 79 416.83 0.67 405 791 794 3 845.92 0.38 406 792 795 3 858.93 6.88 407 792 796 4 835.83 4.74 408 793 796 3 840.53 2.39 409 793 797 4 799.09 7.23 410 793 862 69 416.94 0.60 411 794 798 4 777.09 3.73 412 794 799 5 601.25 5.85 413 794 804 10 477.75 30.83 414 794 862 68 416.99 0.59 415 795 798 3 782.38 12.06 416 797 855 58 487.27 10.19 417 797 858 61 473.26 6.18 418 797 862 65 431.63 7.84 419 798 855 57 488.86 10.63 420 799 803 4 830.73 12.59 421 799 804 5 811.32 2.88 422 799 807 8 800.15 2.02 423 799 855 56 489.18 10.52 424 799 859 60 487.47 10.12 425 799 862 63 480.96 6.90 426 800 803 3 850.91 6.30 427 800 804 4 845.67 5.12 428 801 804 3 849.49 7.02 429 801 805 4 845.67 5.12 430 802 805 3 850.88 2.52 431 802 806 4 845.69 5.11 432 802 841 39 491.75 10.90 433 803 806 3 855.68 0.11 434 803 807 4 845.69 5.10 435 803 841 38 491.50 11.03 436 803 855 52 491.42 11.05 437 803 859 56 490.77 11.14 438 804 807 3 843.83 2.34 439 804 808 4 840.78 2.30 440 805 808 3 843.03 1.94 441 805 809 4 842.27 1.56 442 806 809 3 852.14 3.04 443 806 810 4 841.58 2.68 444 806 834 28 492.92 11.20 445 806 837 31 492.45 11.20 446 806 838 32 492.24 11.13 447 806 841 35 492.24 11.13 448 807 810 3 846.51 0.23 449 807 811 4 843.63 1.66 450 807 838 31 492.28 11.15 451 807 859 52 491.01 11.09 452 807 862 55 490.95 11.06 453 807 863 56 490.60 11.19 454 808 811 3 846.51 0.23 455 808 812 4 843.63 1.66 456 809 812 3 845.81 0.18 457 809 813 4 842.42 1.04 458 809 834 25 493.19 11.10 459 810 813 3 842.88 1.28 460 810 814 4 839.53 0.39 461 810 831 21 493.23 11.12 462 810 834 24 493.19 11.10 463 810 835 25 493.19 11.09 464 810 838 28 493.18 11.09 465 810 863 53 490.95 11.07 466 810 916 106 460.33 6.93 467 811 814 3 837.55 0.62 468 811 863 52 490.94 11.06 469 811 866 55 490.69 11.08 470 811 867 56 490.63 11.10 471 813 827 14 493.77 10.92 472 813 830 17 493.72 10.94 473 813 831 18 493.67 10.95 474 813 834 21 493.65 10.95 475 814 831 17 493.72 10.94 476 814 867 53 490.71 11.09 477 814 913 99 461.12 6.90 478 814 914 100 460.61 7.05 479 815 906 91 463.75 7.17 480 815 911 96 462.14 6.89 481 815 912 97 461.66 6.91 482 815 913 98 461.12 6.90 483 816 821 5 677.25 16.43 484 816 824 8 498.93 8.85 485 816 827 11 494.08 10.84 486 816 911 95 462.15 6.89 487 816 912 96 462.13 6.89 488 816 914 98 461.32 6.96 489 817 820 3 824.72 0.85 490 817 821 4 786.33 16.65 491 817 910 93 463.29 7.12 492 817 911 94 462.35 6.79 493 818 909 91 463.76 7.16 494 818 910 92 463.51 7.07 495 818 912 94 462.50 6.73 496 820 824 4 735.78 6.82 497 821 824 3 823.25 0.55 498 821 827 6 748.47 0.46 499 823 826 3 855.44 2.20 500 823 827 4 852.73 3.57 501 824 827 3 852.74 3.57 502 824 828 4 841.63 9.12 503 825 828 3 858.36 0.76 504 825 829 4 845.37 7.25 505 826 829 3 858.36 0.77 506 826 830 4 841.62 9.12 507 827 830 3 840.34 9.78 508 827 831 4 841.61 9.12 509 827 914 87 461.93 6.87 510 828 831 3 852.59 3.63 511 828 832 4 822.18 7.98 512 829 832 3 841.67 1.82 513 829 833 4 845.80 0.23 514 830 833 3 852.64 3.12 515 830 834 4 846.65 0.65 516 831 834 3 847.98 1.30 517 831 835 4 845.81 0.23 518 831 914 83 460.91 7.01 519 831 916 85 460.47 6.86 520 832 835 3 845.82 0.23 521 832 836 4 845.80 0.23 522 833 836 3 844.86 0.68 523 833 837 4 839.75 3.23 524 834 837 3 830.71 1.28 525 834 838 4 830.71 1.29 526 835 838 3 839.26 5.56 527 835 839 4 830.71 1.29 528 835 856 21 492.03 11.02 529 835 916 81 460.12 6.99 530 836 839 3 839.64 4.11 531 836 840 4 837.97 4.95 532 837 840 3 840.34 3.77 533 837 841 4 826.24 0.81 534 838 841 3 840.60 3.64 535 838 842 4 830.31 1.23 536 838 856 18 491.88 11.00 537 838 859 21 491.63 11.04 538 839 842 3 832.10 0.34 539 839 843 4 827.85 0.01 540 839 856 17 491.88 11.00 541 840 843 3 828.99 0.56 542 840 844 4 827.83 0.03 543 841 844 3 838.66 5.38 544 842 846 4 819.80 4.04 545 842 855 13 492.06 11.02 546 842 856 14 492.05 11.02 547 842 859 17 492.04 11.01 548 843 852 9 493.92 11.89 549 846 851 5 645.00 19.88 550 846 852 6 538.22 1.84 551 846 855 9 544.66 1.38 552 848 851 3 865.12 1.62 553 848 852 4 815.95 5.57 554 849 852 3 833.02 2.98 555 849 853 4 825.12 0.97 556 850 854 4 825.28 1.00 557 851 854 3 831.13 1.09 558 851 855 4 825.33 1.02 559 852 855 3 841.37 3.39 560 852 856 4 825.34 1.02 561 853 856 3 845.37 1.39 562 854 857 3 831.43 3.67 563 854 858 4 825.77 0.82 564 855 858 3 844.71 3.85 565 855 859 4 825.78 0.81 566 856 859 3 855.26 1.43 567 856 860 4 830.49 3.16 568 857 860 3 852.59 0.09 569 857 861 4 830.50 3.16 570 857 917 60 459.89 6.91 571 858 861 3 845.06 3.85 572 858 862 4 819.25 2.46 573 859 862 3 828.61 5.30 574 859 863 4 837.87 0.67 575 860 863 3 845.58 3.18 576 860 864 4 837.87 0.67 577 860 916 56 460.10 7.00 578 860 917 57 460.06 6.98 579 861 864 3 837.88 0.67 580 861 865 4 837.87 0.67 581 861 917 56 459.91 6.91 582 862 865 3 839.38 1.15 583 862 866 4 847.34 2.83 584 863 866 3 851.46 0.80 585 863 916 53 460.17 7.01 586 864 867 3 855.29 1.18 587 864 868 4 855.29 1.18 588 864 915 51 460.19 7.00 589 864 916 52 460.17 7.01 590 864 917 53 460.17 7.01 591 865 868 3 863.38 2.87 592 865 869 4 852.98 0.03 593 866 869 3 855.29 1.18 594 866 870 4 834.38 1.46 595 867 870 3 841.37 0.01 596 867 871 4 834.37 1.46 597 867 913 46 460.84 7.02 598 867 915 48 460.75 6.99 599 868 871 3 845.24 0.56 600 868 872 4 844.03 1.32 601 868 915 47 460.76 7.00 602 869 872 3 852.20 2.51 603 869 873 4 844.03 1.32 604 870 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