BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 671 840 169 22.27 0.15 2 672 675 3 848.49 2.97 3 672 676 4 841.76 7.42 4 673 676 3 858.46 0.93 5 673 677 4 841.76 7.42 6 673 723 50 26.51 0.96 7 673 806 133 24.25 0.01 8 673 834 161 24.08 0.06 9 673 837 164 24.10 0.07 10 674 677 3 855.51 0.55 11 674 678 4 836.88 9.87 12 674 802 128 24.69 0.09 13 674 841 167 26.50 1.10 14 675 678 3 849.23 4.11 15 675 679 4 828.36 5.66 16 676 679 3 851.93 2.32 17 676 680 4 828.12 5.78 18 676 721 45 31.52 0.12 19 676 723 47 28.61 1.32 20 677 680 3 846.60 4.99 21 677 681 4 809.44 5.85 22 677 722 45 31.48 0.11 23 677 805 128 27.93 1.52 24 677 806 129 27.93 1.53 25 677 809 132 27.93 1.52 26 678 681 3 841.62 6.87 27 678 802 124 28.89 1.14 28 678 805 127 31.11 0.04 29 680 718 38 31.71 0.17 30 680 721 41 31.70 0.17 31 680 722 42 31.64 0.17 32 681 718 37 32.11 0.01 33 681 805 124 31.52 0.13 34 681 808 127 31.54 0.14 35 682 714 32 34.63 0.27 36 682 718 36 32.24 0.04 37 684 766 82 81.74 0.44 38 685 707 22 81.85 0.49 39 685 710 25 81.33 0.36 40 685 711 26 77.50 0.53 41 685 714 29 77.20 0.69 42 685 765 80 81.33 0.27 43 685 766 81 81.88 0.48 44 686 707 21 81.88 0.48 45 686 710 24 81.89 0.49 46 686 765 79 81.86 0.49 47 686 767 81 81.90 0.48 48 687 703 16 81.92 0.48 49 687 706 19 81.91 0.49 50 687 707 20 81.88 0.48 51 687 710 23 81.90 0.49 52 687 765 78 81.89 0.49 53 687 767 80 81.97 0.49 54 687 768 81 81.92 0.48 55 688 710 22 81.95 0.49 56 688 767 79 82.03 0.48 57 689 700 11 82.05 0.50 58 689 703 14 82.00 0.51 59 689 767 78 82.05 0.48 60 689 768 79 82.03 0.48 61 689 774 85 143.03 30.95 62 690 693 3 870.14 3.64 63 691 767 76 152.40 35.63 64 691 774 83 272.12 88.91 65 695 700 5 792.28 1.52 66 695 773 78 303.45 91.06 67 695 774 79 301.10 89.91 68 695 777 82 303.66 90.97 69 696 699 3 844.16 4.71 70 696 700 4 826.46 4.14 71 697 700 3 844.17 4.71 72 697 701 4 826.50 4.13 73 697 777 80 308.57 92.76 74 697 778 81 304.33 90.64 75 697 779 82 307.51 92.23 76 697 880 183 171.24 14.05 77 697 887 190 170.01 13.04 78 697 889 192 147.20 1.64 79 698 701 3 855.31 5.30 80 698 702 4 830.68 1.94 81 698 887 189 170.28 13.18 82 698 888 190 170.01 13.04 83 698 889 191 147.18 1.64 84 699 702 3 833.87 0.43 85 699 703 4 833.79 0.40 86 700 703 3 833.59 0.48 87 700 704 4 825.66 4.45 88 700 768 68 326.27 90.98 89 700 770 70 326.23 91.06 90 700 774 74 326.25 91.07 91 700 777 77 326.63 90.91 92 700 778 78 326.25 91.08 93 701 704 3 830.43 2.04 94 701 705 4 825.64 4.44 95 701 778 77 326.64 90.90 96 701 780 79 326.26 91.08 97 701 876 175 184.17 20.04 98 701 880 179 172.21 13.97 99 701 889 188 173.28 12.59 100 702 705 3 833.12 0.74 101 702 706 4 825.63 4.43 102 702 889 187 173.28 12.59 103 702 890 188 173.44 12.51 104 703 706 3 844.05 4.75 105 703 707 4 831.82 1.32 106 703 768 65 330.51 89.30 107 704 707 3 831.84 1.31 108 704 708 4 831.84 1.31 109 704 764 60 329.20 89.80 110 704 768 64 331.93 88.64 111 704 770 66 330.88 89.48 112 704 780 76 329.98 90.14 113 705 708 3 817.76 5.73 114 705 709 4 824.32 7.65 115 705 889 184 182.37 8.71 116 705 890 185 181.09 9.31 117 705 891 186 180.33 9.62 118 705 892 187 180.47 9.01 119 705 895 190 177.71 8.79 120 706 709 3 837.89 1.63 121 706 710 4 824.32 7.65 122 706 892 186 182.13 8.18 123 707 710 3 838.48 0.57 124 707 711 4 824.32 7.65 125 707 764 57 335.68 87.50 126 707 765 58 336.75 87.17 127 707 768 61 336.89 87.24 128 708 711 3 835.84 1.89 129 708 712 4 836.36 1.62 130 708 745 37 334.98 87.14 131 708 895 187 182.19 6.55 132 709 712 3 840.41 3.64 133 709 713 4 832.45 0.40 134 709 892 183 184.91 7.40 135 709 893 184 184.91 7.38 136 709 894 185 184.18 7.05 137 709 895 186 183.28 6.69 138 710 713 3 846.58 3.48 139 710 714 4 832.49 0.42 140 711 714 3 837.38 2.02 141 711 715 4 831.75 0.76 142 711 745 34 340.54 85.42 143 711 752 41 340.75 85.41 144 712 715 3 831.78 0.77 145 712 716 4 833.75 0.21 146 712 737 25 341.81 84.90 147 712 741 29 340.62 85.28 148 712 745 33 340.81 85.38 149 712 894 182 185.91 6.88 150 712 895 183 185.84 6.87 151 712 898 186 183.95 5.92 152 713 716 3 841.52 0.05 153 713 717 4 833.75 0.21 154 713 894 181 186.00 6.86 155 714 717 3 835.24 0.95 156 714 718 4 832.58 0.79 157 715 718 3 833.66 0.24 158 715 719 4 832.53 0.79 159 715 737 22 344.34 83.65 160 715 744 29 341.87 84.88 161 715 745 30 341.86 84.89 162 716 719 3 833.33 0.39 163 716 720 4 827.80 1.57 164 716 737 21 344.33 83.64 165 717 720 3 838.02 2.32 166 717 721 4 826.89 3.25 167 718 721 3 840.10 1.05 168 718 722 4 825.97 3.71 169 718 744 26 347.61 82.12 170 718 808 90 347.58 82.12 171 718 809 91 347.57 82.12 172 718 812 94 347.56 82.08 173 719 722 3 836.89 2.92 174 719 725 6 562.25 13.74 175 719 737 18 347.75 81.98 176 719 812 93 347.55 82.07 177 720 725 5 596.58 3.52 178 720 726 6 517.70 9.45 179 720 733 13 348.22 81.98 180 722 725 3 847.66 2.42 181 722 809 87 348.05 81.90 182 722 812 90 348.06 81.89 183 722 834 112 265.72 40.72 184 723 830 107 266.10 40.56 185 723 834 111 265.81 40.68 186 724 727 3 839.98 0.75 187 724 830 106 266.11 40.55 188 725 728 3 868.59 7.46 189 725 733 8 536.27 0.04 190 725 736 11 584.20 24.08 191 725 737 12 536.16 0.07 192 725 812 87 348.92 81.59 193 725 813 88 348.81 81.54 194 725 830 105 266.47 40.37 195 726 733 7 648.26 4.25 196 727 826 99 274.19 43.95 197 728 732 4 824.93 2.15 198 728 733 5 808.43 0.35 199 728 813 85 349.22 81.47 200 728 826 98 274.19 43.94 201 728 827 99 274.18 43.95 202 728 830 102 274.13 43.93 203 729 732 3 839.77 4.32 204 729 733 4 825.49 2.42 205 729 822 93 291.76 35.66 206 730 733 3 840.32 4.04 207 730 734 4 826.00 2.67 208 731 734 3 841.49 4.46 209 731 735 4 825.53 2.43 210 731 822 91 297.80 32.64 211 732 735 3 830.46 0.04 212 732 736 4 825.53 2.42 213 732 821 89 297.80 32.65 214 732 822 90 297.79 32.63 215 732 823 91 298.94 32.05 216 733 736 3 852.92 2.33 217 733 737 4 826.42 1.97 218 734 737 3 838.59 2.19 219 734 738 4 836.28 3.34 220 735 738 3 837.21 2.89 221 735 739 4 836.28 3.35 222 735 821 86 304.12 29.48 223 736 739 3 836.75 3.58 224 736 740 4 821.81 3.89 225 736 812 76 373.92 69.15 226 736 816 80 315.09 39.74 227 736 821 85 309.30 26.88 228 736 827 91 301.48 28.51 229 737 741 4 804.84 1.68 230 737 812 75 379.42 66.41 231 738 741 3 845.00 0.73 232 738 898 160 235.65 20.18 233 738 902 164 228.86 20.88 234 739 815 76 385.60 63.27 235 739 817 78 329.20 32.66 236 739 902 163 231.06 22.48 237 739 905 166 226.72 21.96 238 739 906 167 220.63 19.34 239 739 911 172 205.00 27.04 240 740 743 3 842.97 2.67 241 740 744 4 820.63 0.98 242 740 811 71 385.69 63.31 243 740 812 72 385.68 63.30 244 740 815 75 326.81 33.87 245 741 744 3 845.65 1.33 246 741 745 4 828.15 3.15 247 741 895 154 241.48 20.91 248 741 898 157 238.03 21.41 249 741 899 158 238.33 21.57 250 741 903 162 231.50 23.17 251 742 745 3 842.02 3.15 252 742 746 4 828.18 3.16 253 742 870 128 258.99 9.34 254 742 873 131 259.00 9.42 255 742 874 132 258.99 9.39 256 742 877 135 261.00 10.48 257 743 746 3 854.96 4.03 258 743 747 4 826.63 3.93 259 743 811 68 395.12 58.79 260 743 866 123 259.10 9.22 261 743 870 127 259.00 9.35 262 744 747 3 854.96 4.03 263 744 748 4 828.19 3.16 264 744 808 64 395.16 58.78 265 744 811 67 395.16 58.77 266 744 812 68 395.25 58.73 267 745 748 3 855.31 4.45 268 745 749 4 816.64 0.69 269 746 749 3 826.38 4.07 270 746 750 4 834.63 0.29 271 746 787 41 410.69 61.09 272 746 790 44 410.53 60.98 273 746 869 123 264.26 11.94 274 746 873 127 264.31 12.08 275 747 750 3 845.94 5.84 276 747 751 4 839.14 1.96 277 747 790 43 410.56 60.97 278 747 794 47 410.67 60.84 279 747 804 57 401.00 56.05 280 747 807 60 400.81 56.13 281 747 808 61 400.75 56.11 282 747 811 64 400.74 56.11 283 747 862 115 273.81 7.23 284 747 866 119 264.44 11.95 285 748 751 3 850.35 3.64 286 748 752 4 839.14 1.96 287 748 804 56 400.99 56.06 288 748 808 60 400.87 56.04 289 749 752 3 847.50 1.88 290 749 753 4 839.13 1.96 291 749 762 13 414.28 61.83 292 749 763 14 414.28 61.83 293 749 764 15 414.25 61.82 294 749 787 38 411.35 60.81 295 750 753 3 839.16 1.96 296 750 754 4 839.14 1.96 297 750 791 41 410.91 60.79 298 750 794 44 410.81 60.77 299 751 754 3 835.65 0.22 300 751 755 4 834.59 0.25 301 751 794 43 411.07 60.63 302 751 799 48 401.75 56.01 303 751 801 50 401.70 55.98 304 751 804 53 401.16 55.97 305 752 755 3 839.49 2.69 306 752 756 4 806.84 4.32 307 752 763 11 414.36 61.83 308 752 764 12 414.39 61.84 309 753 756 3 834.72 0.28 310 753 757 4 806.81 4.31 311 753 758 5 719.43 10.31 312 753 759 6 547.46 7.67 313 753 761 8 414.64 61.88 314 753 762 9 414.53 61.92 315 753 763 10 414.36 61.83 316 753 791 38 411.46 60.72 317 754 758 4 663.64 7.93 318 754 759 5 547.43 7.71 319 754 791 37 411.54 60.73 320 754 794 40 411.23 60.61 321 754 795 41 411.21 60.59 322 756 763 7 516.01 11.30 323 757 760 3 855.82 2.94 324 757 761 4 758.33 8.99 325 757 763 6 516.85 11.71 326 759 788 29 411.80 60.72 327 759 791 32 411.69 60.68 328 759 792 33 411.62 60.63 329 761 769 8 415.18 61.97 330 761 770 9 414.97 62.02 331 761 771 10 414.88 62.06 332 762 769 7 415.21 61.98 333 762 770 8 414.99 62.02 334 762 775 13 414.99 62.02 335 762 784 22 411.79 60.73 336 762 787 25 411.77 60.72 337 762 788 26 411.69 60.69 338 763 766 3 896.88 0.90 339 763 769 6 415.21 61.98 340 764 768 4 792.83 13.74 341 764 770 6 567.86 16.82 342 764 787 23 411.76 60.72 343 765 768 3 820.76 0.23 344 765 769 4 792.84 13.73 345 768 774 6 828.76 3.43 346 769 774 5 828.77 3.43 347 770 774 4 828.73 3.45 348 770 775 5 818.74 1.54 349 770 778 8 737.29 10.71 350 771 774 3 847.77 4.77 351 771 775 4 828.77 3.43 352 772 775 3 836.96 0.66 353 772 776 4 834.99 0.32 354 772 781 9 585.49 15.05 355 772 788 16 532.56 0.40 356 773 776 3 853.66 1.91 357 773 777 4 828.92 3.35 358 774 777 3 834.64 0.43 359 774 778 4 828.85 3.33 360 775 778 3 851.31 0.72 361 775 780 5 769.01 0.99 362 775 781 6 585.45 15.06 363 775 784 9 514.62 9.75 364 775 788 13 532.97 0.58 365 776 779 3 853.51 1.82 366 776 781 5 594.45 10.58 367 778 876 98 314.87 46.98 368 779 876 97 314.87 46.98 369 779 887 108 306.49 53.41 370 780 783 3 856.19 0.51 371 780 784 4 822.50 0.21 372 780 787 7 801.50 3.15 373 780 876 96 314.94 47.02 374 781 784 3 859.38 6.37 375 781 785 4 822.50 0.21 376 782 785 3 847.11 3.56 377 782 786 4 844.58 4.82 378 783 786 3 851.62 2.19 379 783 787 4 827.68 3.62 380 783 872 89 324.43 42.50 381 783 873 90 325.24 42.91 382 783 876 93 318.80 47.25 383 784 787 3 833.69 0.62 384 784 788 4 828.42 4.00 385 785 788 3 834.77 0.89 386 785 789 4 827.69 3.64 387 786 789 3 819.08 0.34 388 786 790 4 806.18 5.57 389 786 868 82 325.81 42.72 390 786 869 83 325.81 42.72 391 786 872 86 326.67 43.33 392 787 790 3 832.35 7.51 393 787 791 4 815.38 3.71 394 787 869 82 325.79 42.72 395 787 873 86 326.72 43.30 396 788 791 3 818.72 2.04 397 788 792 4 812.64 2.33 398 789 792 3 837.24 5.33 399 789 793 4 819.17 0.95 400 790 793 3 839.44 2.86 401 790 794 4 838.60 3.35 402 790 862 72 335.10 38.29 403 790 866 76 326.79 43.13 404 790 869 79 326.86 43.24 405 791 794 3 855.16 4.24 406 792 795 3 865.21 3.75 407 792 796 4 851.50 3.10 408 793 796 3 871.91 0.39 409 793 797 4 822.83 11.24 410 793 862 69 335.94 38.69 411 794 798 4 803.08 1.71 412 794 799 5 556.59 0.32 413 794 862 68 335.99 38.69 414 795 798 3 847.80 1.25 415 797 855 58 363.95 51.80 416 797 858 61 360.60 50.30 417 797 862 65 336.95 38.58 418 798 855 57 364.50 51.66 419 799 803 4 845.49 5.21 420 799 804 5 826.04 4.48 421 799 807 8 776.10 0.58 422 799 855 56 364.69 51.74 423 799 859 60 363.87 51.94 424 799 862 63 363.72 51.95 425 800 803 3 850.91 6.30 426 800 804 4 845.64 5.13 427 801 804 3 853.96 4.79 428 801 805 4 845.64 5.13 429 802 805 3 847.02 4.45 430 802 806 4 845.66 5.12 431 802 841 39 389.24 40.50 432 803 806 3 855.68 0.11 433 803 807 4 832.65 1.40 434 803 841 38 389.25 40.51 435 803 855 52 365.41 51.95 436 803 859 56 364.25 52.12 437 804 807 3 843.83 2.34 438 804 808 4 840.80 2.29 439 805 808 3 848.55 0.82 440 805 809 4 840.84 2.31 441 806 809 3 848.68 4.76 442 806 810 4 845.70 0.10 443 806 834 28 408.51 31.23 444 806 837 31 405.94 32.44 445 806 838 32 398.39 36.05 446 806 841 35 389.37 40.55 447 807 810 3 846.43 0.26 448 807 811 4 846.52 0.21 449 807 838 31 398.41 36.05 450 807 859 52 364.39 52.20 451 807 862 55 364.29 52.15 452 807 863 56 363.92 52.16 453 808 811 3 846.57 0.20 454 808 812 4 846.57 0.19 455 809 812 3 846.58 0.18 456 809 813 4 845.97 0.12 457 809 834 25 408.53 31.27 458 810 813 3 853.65 3.73 459 810 814 4 845.96 0.12 460 810 831 21 408.78 31.35 461 810 834 24 408.53 31.27 462 810 835 25 408.52 31.27 463 810 838 28 399.34 35.84 464 810 863 53 364.28 52.20 465 810 916 106 296.22 75.08 466 811 814 3 834.15 6.02 467 811 863 52 364.14 52.27 468 811 866 55 363.52 52.54 469 811 867 56 363.49 52.52 470 813 827 14 409.48 31.11 471 813 830 17 409.42 31.12 472 813 831 18 409.01 31.29 473 813 834 21 408.85 31.40 474 814 831 17 409.35 31.18 475 814 867 53 363.51 52.54 476 814 913 99 296.81 75.26 477 814 914 100 296.24 75.34 478 815 906 91 316.06 66.87 479 815 911 96 304.30 72.54 480 815 912 97 298.95 74.51 481 815 913 98 296.58 75.37 482 816 821 5 621.28 26.44 483 816 824 8 421.93 29.52 484 816 827 11 417.88 27.80 485 816 911 95 304.93 72.23 486 816 912 96 304.11 72.45 487 816 914 98 297.78 74.86 488 817 820 3 821.33 0.84 489 817 821 4 777.21 21.21 490 817 910 93 312.00 68.73 491 817 911 94 305.80 71.79 492 818 909 91 316.08 67.00 493 818 910 92 314.37 67.56 494 818 912 94 311.66 68.89 495 820 824 4 784.94 10.48 496 821 824 3 812.00 5.08 497 821 827 6 764.17 20.43 498 823 826 3 859.11 0.42 499 823 827 4 852.73 3.58 500 824 827 3 852.74 3.58 501 824 828 4 841.62 9.12 502 825 828 3 858.37 0.77 503 825 829 4 845.38 7.25 504 826 829 3 858.37 0.77 505 826 830 4 840.34 9.77 506 827 830 3 845.38 7.25 507 827 831 4 836.31 11.78 508 827 914 87 305.20 71.50 509 828 831 3 846.63 0.66 510 828 832 4 825.26 6.27 511 829 832 3 846.63 0.66 512 829 833 4 841.57 1.89 513 830 833 3 845.78 0.21 514 830 834 4 840.34 2.50 515 831 834 3 847.98 1.30 516 831 835 4 845.80 0.23 517 831 914 83 299.18 74.08 518 831 916 85 298.11 74.45 519 832 835 3 845.80 0.23 520 832 836 4 845.80 0.23 521 833 836 3 846.32 1.41 522 833 837 4 839.83 3.21 523 834 837 3 839.83 3.21 524 834 838 4 839.82 3.21 525 835 838 3 834.36 0.48 526 835 839 4 830.76 1.32 527 835 856 21 365.70 52.02 528 835 916 81 296.43 74.97 529 836 839 3 830.99 0.23 530 836 840 4 837.99 4.93 531 837 840 3 839.50 4.18 532 837 841 4 828.00 0.06 533 838 841 3 837.11 4.58 534 838 842 4 828.03 0.04 535 838 856 18 365.56 52.00 536 838 859 21 365.47 51.98 537 839 842 3 829.69 0.87 538 839 843 4 815.58 6.17 539 840 843 3 845.06 6.82 540 840 844 4 822.99 2.48 541 841 844 3 826.89 0.52 542 842 846 4 806.71 9.57 543 842 852 10 365.80 52.03 544 842 855 13 365.73 52.00 545 842 856 14 365.69 52.01 546 842 859 17 365.69 52.01 547 843 846 3 839.02 4.86 548 843 852 9 376.94 57.44 549 846 851 5 744.78 7.08 550 846 852 6 717.25 16.71 551 846 855 9 556.47 17.70 552 848 851 3 860.20 0.83 553 848 852 4 822.22 1.25 554 849 852 3 846.60 2.85 555 849 853 4 823.83 0.45 556 850 853 3 855.41 1.56 557 850 854 4 828.28 0.33 558 851 854 3 834.58 4.94 559 851 855 4 824.77 0.04 560 852 855 3 841.37 3.39 561 852 856 4 831.73 2.67 562 853 856 3 845.38 1.39 563 853 857 4 830.88 3.10 564 854 857 3 832.58 3.09 565 854 858 4 827.20 1.26 566 855 858 3 845.84 3.28 567 855 859 4 827.19 1.27 568 856 859 3 852.58 0.09 569 856 860 4 825.90 1.91 570 857 860 3 845.80 3.48 571 857 861 4 834.46 2.39 572 857 917 60 295.95 75.06 573 858 861 3 841.19 5.79 574 858 862 4 828.05 5.60 575 859 862 3 834.45 2.41 576 859 863 4 837.88 0.68 577 860 863 3 837.90 0.68 578 860 864 4 844.81 4.13 579 860 916 56 295.74 75.17 580 860 917 57 295.75 75.17 581 861 864 3 845.72 3.68 582 861 865 4 844.81 4.13 583 861 917 56 295.74 75.18 584 862 865 3 847.77 2.66 585 862 866 4 847.87 2.60 586 863 866 3 851.48 0.81 587 863 867 4 850.15 3.75 588 863 916 53 295.85 75.23 589 864 867 3 861.16 1.75 590 864 868 4 855.30 1.18 591 864 915 51 295.87 75.23 592 864 916 52 295.85 75.23 593 864 917 53 295.82 75.22 594 865 868 3 863.81 2.66 595 865 869 4 855.29 1.18 596 866 869 3 855.72 1.40 597 866 870 4 841.43 0.43 598 867 870 3 843.80 0.74 599 867 871 4 841.84 0.23 600 867 913 46 296.50 75.22 601 867 915 48 296.14 75.38 602 868 871 3 844.03 1.32 603 868 872 4 841.84 0.23 604 868 915 47 296.49 75.53 605 869 872 3 852.21 2.50 606 869 873 4 841.84 0.22 607 870 873 3 835.58 5.80 608 870 874 4 826.95 1.49 609 871 874 3 842.24 2.48 610 871 875 4 826.81 1.56 611 871 907 36 313.42 69.17 612 872 875 3 853.25 2.68 613 872 876 4 815.39 7.26 614 873 876 3 823.89 11.51 615 873 877 4 807.59 0.95 616 874 877 3 852.87 2.90 617 874 878 4 817.77 4.08 618 874 903 29 317.18 67.35 619 874 906 32 315.78 68.05 620 874 907 33 313.47 69.20 621 875 878 3 848.13 0.53 622 875 879 4 822.60 1.78 623 875 907 32 313.27 69.30 624 876 879 3 855.03 7.08 625 876 880 4 822.60 1.78 626 877 880 3 840.36 7.08 627 877 881 4 823.25 1.47 628 877 891 14 328.19 64.22 629 877 899 22 322.39 65.18 630 878 881 3 852.33 3.54 631 878 882 4 823.25 1.47 632 878 899 21 322.42 65.17 633 878 903 25 317.54 67.51 634 878 904 26 317.48 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