BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 672 675 3 829.83 1.44 2 672 676 4 824.51 1.38 3 673 676 3 850.69 4.92 4 673 677 4 835.58 4.16 5 673 723 50 27.22 0.27 6 673 806 133 26.89 0.28 7 673 834 161 26.82 0.24 8 673 837 164 26.62 0.34 9 674 677 3 850.61 3.35 10 674 678 4 835.52 4.19 11 674 802 128 26.91 0.29 12 674 841 167 26.89 0.28 13 675 678 3 857.78 2.74 14 675 679 4 824.62 1.28 15 676 679 3 850.61 3.35 16 676 680 4 824.62 1.28 17 676 723 47 29.83 0.88 18 677 680 3 853.27 2.62 19 677 681 4 824.62 1.28 20 677 722 45 32.84 0.14 21 677 805 128 29.41 0.73 22 677 806 129 29.41 0.73 23 677 809 132 29.41 0.73 24 678 681 3 842.91 2.87 25 678 805 127 32.81 0.14 26 680 718 38 33.87 0.60 27 680 721 41 33.88 0.60 28 680 722 42 33.84 0.60 29 681 718 37 34.04 0.56 30 681 804 123 33.88 0.62 31 681 805 124 33.87 0.62 32 681 808 127 33.94 0.59 33 682 714 32 36.66 0.49 34 682 718 36 35.05 1.05 35 684 766 82 86.69 0.36 36 685 707 22 86.74 0.33 37 685 710 25 86.62 0.29 38 685 711 26 81.28 0.76 39 685 714 29 81.29 0.76 40 685 765 80 86.61 0.39 41 685 766 81 86.77 0.33 42 686 707 21 86.77 0.32 43 686 710 24 86.78 0.32 44 686 765 79 86.68 0.35 45 686 766 80 86.77 0.33 46 686 767 81 86.79 0.34 47 687 703 16 86.83 0.31 48 687 706 19 86.80 0.30 49 687 707 20 86.77 0.32 50 687 710 23 86.80 0.30 51 687 765 78 86.77 0.33 52 687 767 80 87.15 0.17 53 687 768 81 86.80 0.35 54 688 710 22 87.17 0.18 55 688 767 79 87.24 0.18 56 689 693 4 872.76 0.26 57 689 700 11 87.28 0.21 58 689 703 14 87.22 0.18 59 689 767 78 87.25 0.18 60 689 768 79 87.24 0.18 61 689 774 85 87.32 0.20 62 690 693 3 872.76 0.26 63 691 767 76 87.28 0.16 64 691 774 83 97.01 1.47 65 693 699 6 778.37 2.94 66 694 699 5 814.00 5.38 67 695 700 5 805.45 1.10 68 695 773 78 125.14 0.77 69 695 774 79 123.84 0.55 70 695 777 82 125.12 0.78 71 696 699 3 840.79 4.95 72 696 700 4 824.25 1.40 73 696 777 81 125.25 0.75 74 697 700 3 848.18 1.71 75 697 701 4 824.26 1.41 76 697 777 80 125.27 0.74 77 697 778 81 125.26 0.75 78 697 779 82 125.26 0.75 79 697 880 183 126.55 0.46 80 698 701 3 829.71 1.05 81 698 702 4 826.69 2.56 82 699 702 3 841.94 4.83 83 699 703 4 824.32 1.38 84 700 703 3 836.05 0.04 85 700 704 4 822.51 0.48 86 700 768 68 136.22 1.30 87 700 770 70 136.21 1.30 88 700 774 74 136.20 1.29 89 700 777 77 137.07 0.83 90 700 778 78 136.15 1.28 91 701 704 3 845.00 4.44 92 701 705 4 825.38 3.21 93 701 880 179 136.14 1.28 94 702 705 3 863.98 1.52 95 702 706 4 827.47 0.06 96 703 706 3 825.38 0.98 97 703 707 4 819.94 0.13 98 703 768 65 148.93 0.44 99 704 707 3 832.36 0.32 100 704 708 4 820.51 3.83 101 704 764 60 148.59 0.57 102 704 768 64 149.81 0.85 103 704 770 66 148.93 0.44 104 705 708 3 824.75 1.71 105 705 709 4 820.52 3.83 106 705 892 187 154.75 0.57 107 705 895 190 153.59 0.36 108 706 709 3 855.69 5.33 109 706 710 4 823.55 2.31 110 707 710 3 838.31 3.33 111 707 711 4 826.19 3.64 112 707 764 57 155.83 0.48 113 707 765 58 155.88 0.49 114 707 768 61 155.88 0.49 115 708 711 3 844.88 5.71 116 708 712 4 827.91 2.77 117 708 895 187 164.87 0.03 118 709 712 3 854.65 2.89 119 709 713 4 834.31 5.96 120 709 892 183 167.23 1.15 121 709 893 184 172.83 0.14 122 709 894 185 167.24 1.14 123 709 895 186 167.14 1.11 124 710 713 3 853.11 2.12 125 710 714 4 839.24 2.88 126 711 714 3 845.35 0.55 127 711 715 4 836.59 4.56 128 711 745 34 167.08 1.07 129 711 752 41 167.10 1.07 130 712 715 3 836.99 4.76 131 712 716 4 834.41 6.04 132 712 741 29 167.92 0.79 133 712 894 182 180.16 1.23 134 712 895 183 179.71 1.01 135 712 898 186 179.71 1.01 136 713 716 3 864.56 1.47 137 713 717 4 841.55 2.48 138 713 894 181 180.45 1.33 139 714 717 3 849.42 0.43 140 714 718 4 834.40 6.04 141 715 718 3 843.68 1.70 142 715 719 4 834.27 2.42 143 715 737 22 182.13 0.48 144 715 744 29 180.19 1.21 145 715 745 30 180.19 1.22 146 716 719 3 843.36 1.54 147 716 720 4 832.06 0.62 148 716 734 18 187.41 0.86 149 716 737 21 182.37 0.55 150 717 720 3 840.54 4.83 151 717 721 4 829.51 0.69 152 718 721 3 860.36 2.65 153 718 722 4 840.16 4.94 154 718 744 26 187.76 0.79 155 718 808 90 187.75 0.79 156 718 809 91 187.75 0.79 157 718 812 94 187.79 0.77 158 719 722 3 846.26 1.89 159 719 725 6 613.88 18.80 160 719 737 18 187.83 0.78 161 719 812 93 187.78 0.77 162 720 725 5 560.35 7.98 163 720 726 6 521.48 9.97 164 720 733 13 188.94 0.40 165 720 734 14 188.98 0.42 166 722 725 3 842.24 6.41 167 722 809 87 189.12 0.33 168 722 812 90 189.15 0.34 169 722 813 91 189.16 0.33 170 722 834 112 254.71 32.47 171 723 830 107 254.77 32.47 172 723 834 111 254.73 32.46 173 724 727 3 841.62 3.68 174 724 830 106 335.33 7.82 175 725 728 3 872.51 2.79 176 725 733 8 524.12 8.96 177 725 737 12 519.63 6.73 178 725 812 87 255.39 32.13 179 725 813 88 335.35 7.81 180 725 830 105 352.51 2.20 181 726 733 7 712.72 31.50 182 727 826 99 391.76 1.13 183 728 732 4 824.39 4.01 184 728 733 5 810.84 3.23 185 728 813 85 391.70 1.12 186 728 826 98 401.00 1.38 187 728 827 99 393.84 2.17 188 728 830 102 391.76 1.13 189 729 732 3 847.60 1.49 190 729 733 4 830.55 7.04 191 729 822 93 457.43 6.93 192 729 823 94 432.57 5.54 193 730 733 3 850.07 2.71 194 730 734 4 850.09 2.71 195 731 734 3 855.07 1.27 196 731 735 4 850.07 2.71 197 731 822 91 465.08 7.46 198 732 735 3 850.10 2.71 199 732 736 4 830.52 7.05 200 732 821 89 465.13 7.42 201 732 822 90 465.09 7.46 202 732 823 91 465.08 7.46 203 733 736 3 864.70 3.55 204 733 737 4 836.19 4.21 205 734 737 3 844.76 1.80 206 734 738 4 825.86 5.66 207 735 738 3 840.28 0.45 208 735 739 4 825.86 5.67 209 735 902 167 263.12 0.39 210 736 739 3 860.01 2.23 211 736 740 4 830.34 7.04 212 736 812 76 467.27 6.72 213 736 816 80 467.97 6.55 214 736 821 85 467.78 6.65 215 736 827 91 467.36 6.79 216 737 740 3 846.08 1.17 217 737 741 4 820.98 2.37 218 737 812 75 467.31 6.78 219 738 741 3 841.78 1.35 220 738 897 159 281.59 0.13 221 738 898 160 279.93 0.70 222 738 902 164 266.69 0.61 223 739 815 76 472.85 7.58 224 739 816 77 474.90 8.56 225 739 817 78 477.24 9.70 226 739 902 163 277.68 1.54 227 739 905 166 270.31 1.19 228 739 906 167 266.56 0.68 229 739 911 172 265.94 0.46 230 740 743 3 846.81 5.84 231 740 744 4 831.45 1.07 232 740 811 71 472.72 7.59 233 740 812 72 472.72 7.59 234 740 815 75 474.90 8.56 235 741 744 3 842.69 3.78 236 741 745 4 839.78 5.24 237 741 895 154 283.94 1.05 238 741 898 157 283.85 1.02 239 741 899 158 283.81 1.00 240 742 745 3 842.69 3.79 241 742 746 4 839.78 5.24 242 742 870 128 389.87 3.59 243 742 873 131 379.43 4.11 244 742 874 132 358.69 0.64 245 742 877 135 350.26 3.12 246 743 746 3 850.78 0.26 247 743 747 4 839.76 5.25 248 743 811 68 480.00 8.77 249 743 866 123 408.61 0.15 250 743 870 127 389.88 3.58 251 744 747 3 850.76 0.27 252 744 748 4 839.75 5.24 253 744 808 64 480.11 8.80 254 744 811 67 480.06 8.78 255 744 812 68 480.09 8.77 256 745 748 3 849.20 0.51 257 745 749 4 849.18 0.51 258 746 749 3 849.19 0.51 259 746 750 4 846.51 0.83 260 746 787 41 497.80 11.19 261 746 790 44 497.72 11.23 262 746 866 120 408.64 0.16 263 746 869 123 408.10 0.28 264 746 873 127 385.17 1.30 265 747 750 3 846.53 0.83 266 747 751 4 846.51 0.83 267 747 790 43 497.70 11.23 268 747 794 47 497.45 11.11 269 747 804 57 481.48 8.69 270 747 807 60 481.39 8.65 271 747 808 61 481.37 8.63 272 747 811 64 481.31 8.69 273 747 862 115 411.89 0.39 274 747 866 119 408.63 0.16 275 748 751 3 847.38 4.30 276 748 752 4 841.83 1.52 277 748 804 56 481.51 8.67 278 748 808 60 481.44 8.68 279 749 752 3 851.08 3.10 280 749 753 4 841.77 1.54 281 749 762 13 504.82 8.46 282 749 763 14 505.63 8.87 283 749 764 15 505.62 8.86 284 749 787 38 497.98 11.22 285 750 753 3 832.81 3.04 286 750 754 4 832.82 3.04 287 750 791 41 497.69 11.22 288 750 794 44 497.49 11.09 289 751 754 3 840.62 0.86 290 751 755 4 828.20 5.34 291 751 794 43 497.46 11.10 292 751 799 48 484.12 8.58 293 751 800 49 484.12 8.58 294 751 801 50 484.05 8.60 295 751 804 53 481.61 8.68 296 752 755 3 835.91 1.49 297 752 756 4 828.19 5.35 298 752 763 11 505.90 8.91 299 752 764 12 505.91 8.90 300 753 756 3 840.92 1.02 301 753 757 4 792.79 10.29 302 753 758 5 780.56 16.40 303 753 759 6 540.76 1.52 304 753 761 8 515.18 5.38 305 753 762 9 506.89 8.81 306 753 763 10 505.91 8.90 307 753 791 38 497.98 11.25 308 754 758 4 614.40 38.30 309 754 759 5 540.77 1.52 310 754 791 37 498.06 11.27 311 754 794 40 497.75 11.19 312 754 795 41 497.75 11.19 313 756 763 7 507.07 8.79 314 757 760 3 844.68 4.19 315 757 761 4 777.11 11.94 316 757 763 6 540.73 1.53 317 759 788 29 498.31 11.18 318 759 791 32 498.16 11.23 319 759 792 33 498.06 11.22 320 761 769 8 506.26 8.69 321 761 770 9 506.23 8.70 322 761 771 10 506.22 8.71 323 761 775 14 506.23 8.70 324 762 769 7 506.26 8.69 325 762 770 8 506.24 8.70 326 762 775 13 506.23 8.70 327 762 784 22 498.47 11.17 328 762 787 25 498.34 11.20 329 762 788 26 498.30 11.18 330 763 766 3 895.13 1.69 331 763 769 6 513.88 4.88 332 764 768 4 798.91 10.84 333 764 770 6 652.57 61.10 334 764 787 23 498.31 11.18 335 765 768 3 823.62 1.51 336 765 769 4 817.33 1.63 337 766 769 3 889.03 3.50 338 768 774 6 821.64 1.50 339 769 774 5 829.37 2.36 340 770 774 4 821.64 1.50 341 770 775 5 821.63 1.50 342 770 778 8 804.38 2.25 343 770 784 14 520.81 0.02 344 771 774 3 863.01 5.30 345 771 775 4 829.40 2.36 346 772 775 3 863.58 5.02 347 772 776 4 829.40 2.37 348 772 781 9 615.72 0.29 349 772 788 16 526.09 0.83 350 773 776 3 839.13 0.58 351 773 777 4 829.39 2.37 352 774 777 3 837.22 1.54 353 774 778 4 829.09 2.52 354 775 778 3 848.03 3.89 355 775 779 4 829.83 0.18 356 775 780 5 821.07 1.44 357 775 781 6 580.64 13.51 358 775 784 9 526.39 0.69 359 775 788 13 526.12 0.85 360 776 779 3 829.83 0.18 361 776 781 5 640.16 2.89 362 779 876 97 405.45 0.61 363 780 783 3 853.35 0.49 364 780 784 4 819.87 5.21 365 780 873 93 407.82 0.07 366 780 876 96 400.88 1.82 367 781 784 3 830.18 0.06 368 781 785 4 819.89 5.20 369 782 785 3 838.56 0.28 370 782 786 4 819.89 5.19 371 783 786 3 831.89 0.82 372 783 787 4 823.14 3.20 373 783 872 89 408.42 0.17 374 783 873 90 407.54 0.07 375 784 787 3 827.99 1.43 376 784 788 4 823.28 3.12 377 785 788 3 823.96 3.45 378 785 789 4 825.56 1.99 379 786 789 3 839.36 2.31 380 786 790 4 829.13 2.79 381 786 868 82 411.57 0.34 382 786 869 83 410.65 0.31 383 786 872 86 409.44 0.25 384 787 790 3 841.56 3.42 385 787 791 4 838.58 0.20 386 787 869 82 410.64 0.31 387 787 873 86 408.31 0.49 388 788 791 3 838.61 0.21 389 788 792 4 838.61 0.21 390 789 792 3 838.68 0.18 391 789 793 4 838.61 0.21 392 790 793 3 843.80 1.67 393 790 794 4 838.66 0.18 394 790 862 72 411.95 0.40 395 790 865 75 411.81 0.38 396 790 866 76 411.76 0.36 397 790 869 79 411.14 0.48 398 791 794 3 850.45 1.44 399 792 795 3 845.88 3.66 400 792 796 4 838.42 0.06 401 793 796 3 837.39 2.90 402 793 797 4 811.64 1.67 403 793 799 6 772.93 0.53 404 793 862 69 412.38 0.19 405 794 798 4 772.97 0.51 406 794 799 5 625.07 4.37 407 794 804 10 532.69 2.96 408 794 862 68 412.39 0.20 409 795 798 3 804.17 1.93 410 797 855 58 413.05 0.14 411 797 858 61 412.73 0.20 412 797 862 65 412.69 0.19 413 798 855 57 413.07 0.14 414 799 803 4 814.36 5.75 415 799 804 5 790.38 2.88 416 799 807 8 788.72 2.04 417 799 855 56 413.04 0.14 418 799 859 60 412.68 0.17 419 799 862 63 412.61 0.18 420 800 803 3 838.59 0.13 421 800 804 4 827.38 5.74 422 801 804 3 838.59 0.13 423 801 805 4 833.03 2.91 424 802 805 3 857.65 3.04 425 802 806 4 826.58 0.32 426 802 841 39 478.73 7.63 427 803 806 3 833.02 2.90 428 803 807 4 826.57 0.32 429 803 841 38 477.88 7.97 430 803 855 52 412.78 0.18 431 803 859 56 412.56 0.21 432 804 807 3 832.36 0.80 433 804 808 4 824.38 0.78 434 805 808 3 835.10 1.67 435 805 809 4 832.99 3.04 436 806 809 3 842.04 2.67 437 806 810 4 832.51 3.27 438 806 834 28 481.59 8.68 439 806 837 31 481.45 8.62 440 806 838 32 481.39 8.62 441 807 810 3 838.15 0.46 442 807 811 4 832.08 3.67 443 807 838 31 481.41 8.61 444 807 859 52 411.94 0.41 445 807 862 55 411.91 0.42 446 807 863 56 410.56 0.34 447 808 811 3 838.21 0.76 448 808 812 4 832.09 3.67 449 809 812 3 837.90 2.81 450 809 813 4 837.32 1.05 451 809 834 25 481.67 8.64 452 810 813 3 837.32 1.04 453 810 814 4 830.64 4.37 454 810 831 21 481.73 8.64 455 810 834 24 481.63 8.67 456 810 835 25 481.62 8.67 457 810 838 28 481.58 8.68 458 810 863 53 410.51 0.33 459 811 814 3 831.16 3.81 460 811 863 52 410.51 0.33 461 811 866 55 408.61 0.15 462 811 867 56 408.55 0.14 463 813 827 14 482.12 8.64 464 813 830 17 482.05 8.65 465 813 831 18 481.94 8.66 466 813 834 21 481.94 8.66 467 813 914 101 289.24 0.28 468 814 831 17 482.02 8.66 469 814 867 53 408.42 0.13 470 814 913 99 289.25 0.28 471 814 914 100 289.01 0.26 472 815 906 91 294.53 1.22 473 815 911 96 290.85 0.00 474 815 912 97 290.37 0.02 475 815 913 98 289.24 0.28 476 815 914 99 289.24 0.28 477 816 821 5 674.33 35.05 478 816 824 8 482.94 8.45 479 816 827 11 482.50 8.60 480 816 911 95 290.73 0.12 481 816 912 96 290.65 0.09 482 816 914 98 289.91 0.10 483 817 820 3 810.11 11.83 484 817 821 4 745.49 6.01 485 817 910 93 291.68 0.18 486 817 911 94 290.93 0.03 487 818 909 91 294.23 1.14 488 818 910 92 292.01 0.04 489 818 912 94 291.62 0.15 490 820 824 4 784.78 2.92 491 821 824 3 825.75 6.77 492 821 827 6 780.04 19.71 493 823 826 3 847.94 3.86 494 823 827 4 836.49 1.85 495 824 827 3 836.50 1.85 496 824 828 4 836.49 1.85 497 825 828 3 833.44 0.32 498 825 829 4 832.34 0.23 499 826 829 3 833.42 0.32 500 826 830 4 817.43 7.67 501 827 830 3 844.38 3.35 502 827 831 4 830.16 3.76 503 827 914 87 290.25 0.07 504 828 831 3 830.16 3.76 505 828 832 4 830.19 3.75 506 829 832 3 849.27 4.20 507 829 833 4 830.20 3.73 508 830 833 3 839.26 0.80 509 830 834 4 830.19 3.73 510 831 834 3 847.86 3.50 511 831 835 4 827.38 0.12 512 831 914 83 289.25 0.27 513 831 916 85 289.21 0.27 514 832 835 3 834.69 0.33 515 832 836 4 830.04 3.66 516 833 836 3 829.96 3.70 517 833 837 4 831.84 2.75 518 834 837 3 834.27 3.97 519 834 838 4 818.85 5.35 520 835 838 3 821.62 4.85 521 835 839 4 816.84 6.36 522 835 856 21 411.92 0.42 523 835 916 81 288.50 0.14 524 836 839 3 819.39 5.54 525 836 840 4 840.07 5.19 526 837 840 3 847.30 1.58 527 837 841 4 840.08 5.20 528 838 841 3 844.63 2.92 529 838 842 4 824.52 2.96 530 838 856 18 412.23 0.38 531 838 859 21 412.04 0.47 532 839 842 3 843.21 3.62 533 839 843 4 824.52 2.97 534 840 843 3 844.60 0.58 535 840 844 4 808.82 0.29 536 841 844 3 827.36 9.20 537 842 846 4 783.95 2.85 538 842 852 10 412.87 0.21 539 842 855 13 412.72 0.20 540 842 859 17 412.70 0.19 541 843 846 3 791.75 5.24 542 843 852 9 412.95 0.18 543 846 851 5 701.12 13.98 544 846 852 6 623.11 7.71 545 846 855 9 623.11 7.71 546 848 851 3 847.15 0.60 547 848 852 4 833.94 0.34 548 849 852 3 841.02 3.88 549 849 853 4 833.06 0.20 550 850 853 3 844.36 2.21 551 850 854 4 832.28 0.67 552 851 854 3 841.24 0.20 553 851 855 4 832.37 0.71 554 852 855 3 841.49 0.33 555 852 856 4 841.00 5.03 556 853 856 3 845.96 2.56 557 853 857 4 838.82 6.12 558 854 857 3 838.83 6.12 559 854 858 4 830.17 1.79 560 855 858 3 848.62 3.35 561 855 859 4 830.18 1.80 562 856 859 3 838.09 1.93 563 856 860 4 831.73 1.02 564 857 860 3 846.12 5.95 565 857 861 4 831.76 1.04 566 857 917 60 287.62 0.36 567 858 861 3 842.74 0.40 568 858 862 4 838.68 2.42 569 859 862 3 842.99 0.27 570 859 863 4 838.69 2.42 571 860 863 3 839.84 1.84 572 860 864 4 838.00 2.76 573 860 916 56 287.62 0.36 574 860 917 57 287.61 0.36 575 861 864 3 840.25 0.39 576 861 865 4 840.25 0.39 577 861 917 56 287.56 0.38 578 862 865 3 843.18 1.07 579 862 866 4 842.57 0.75 580 863 866 3 867.53 3.01 581 863 916 53 287.61 0.36 582 864 867 3 868.94 3.71 583 864 868 4 859.35 6.71 584 864 915 51 287.61 0.36 585 864 916 52 287.61 0.36 586 864 917 53 287.56 0.39 587 865 868 3 865.27 6.18 588 865 869 4 846.31 1.11 589 866 869 3 859.35 6.71 590 866 870 4 839.00 1.14 591 867 870 3 841.87 2.56 592 867 871 4 838.98 1.13 593 867 913 46 288.12 0.57 594 867 915 48 287.61 0.36 595 868 871 3 840.32 0.84 596 868 872 4 843.53 1.16 597 868 915 47 287.61 0.36 598 869 872 3 844.77 0.54 599 869 873 4 833.58 3.98 600 870 873 3 843.91 0.96 601 870 874 4 831.09 2.79 602 871 874 3 837.98 3.94 603 871 875 4 831.09 2.79 604 871 906 35 296.16 1.79 605 871 907 36 296.12 1.77 606 872 875 3 829.56 0.20 607 872 876 4 822.53 1.49 608 873 876 3 838.08 4.46 609 873 877 4 822.54 1.48 610 874 877 3 846.44 0.29 611 874 878 4 835.68 5.09 612 874 903 29 296.28 1.82 613 874 906 32 296.22 1.79 614 874 907 33 296.21 1.77 615 875 878 3 839.66 3.10 616 875 879 4 827.28 0.92 617 875 907 32 296.24 1.79 618 876 879 3 844.46 0.70 619 876 880 4 831.29 1.08 620 877 880 3 842.81 4.67 621 877 881 4 835.59 2.75 622 877 899 22 296.64 1.95 623 878 881 3 847.02 2.56 624 878 882 4 830.59 0.26 625 878 899 21 296.71 1.98 626 878 903 25 296.63 1.95 627 878 904 26 296.63 1.96 628 878 907 29 296.58 1.93 629 879 882 3 847.02 2.55 630 879 883 4 841.10 5.51 631 880 883 3 847.02 2.55 632 880 884 4 813.96 8.04 633 880 891 11 297.76 2.00 634 881 884 3 836.49 3.22 635 881 885 4 801.72 1.93 636 881 891 10 297.44 2.17 637 881 899 18 296.73 1.99 638 881 900 19 296.73 1.99 639 882 885 3 843.65 0.35 640 883 886 3 856.10 6.58 641 884 888 4 600.29 3.00 642 884 891 7 299.06 1.66 643 885 889 4 442.93 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