BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 63 62 42.58 0.70 2 2 30 28 38.87 0.62 3 2 61 59 50.71 0.53 4 2 62 60 42.51 0.66 5 2 64 62 42.59 0.70 6 2 66 64 40.35 0.12 7 2 92 90 47.73 0.66 8 2 93 91 43.20 0.31 9 3 59 56 51.87 0.07 10 3 60 57 51.69 0.15 11 3 61 58 51.59 0.10 12 3 62 59 50.82 0.48 13 3 90 87 51.81 0.11 14 3 91 88 51.62 0.20 15 3 92 89 48.93 1.26 16 4 12 8 791.75 0.51 17 4 30 26 50.12 0.40 18 4 35 31 48.54 0.29 19 4 57 53 51.80 0.10 20 4 59 55 51.88 0.07 21 4 60 56 51.70 0.15 22 4 62 58 51.77 0.10 23 4 90 86 51.83 0.11 24 4 91 87 51.77 0.13 25 5 57 52 52.01 0.01 26 5 58 53 52.02 0.01 27 5 59 54 51.99 0.04 28 5 88 83 60.69 0.36 29 5 89 84 52.08 0.02 30 5 90 85 51.83 0.11 31 6 11 5 815.19 2.92 32 6 12 6 798.42 2.83 33 6 15 9 783.14 4.12 34 6 52 46 51.81 0.10 35 6 56 50 52.12 0.03 36 6 57 51 52.03 0.01 37 6 88 82 68.02 0.49 38 6 89 83 56.79 2.31 39 6 235 229 52.01 0.01 40 6 237 231 51.99 0.02 41 6 238 232 52.01 0.01 42 7 56 49 52.62 0.12 43 7 58 51 52.88 0.01 44 7 87 80 89.28 0.24 45 7 88 81 75.68 3.40 46 8 11 3 832.56 5.37 47 8 12 4 819.89 0.58 48 8 85 77 90.60 0.10 49 8 87 79 90.12 0.20 50 8 237 229 52.30 0.13 51 9 12 3 844.32 0.51 52 9 13 4 819.96 0.58 53 9 83 74 90.57 0.10 54 9 84 75 90.70 0.14 55 9 87 78 90.14 0.21 56 9 88 79 89.28 0.24 57 9 89 80 89.16 0.18 58 9 102 93 89.65 0.43 59 10 13 3 834.98 4.16 60 10 14 4 826.24 0.21 61 10 83 73 90.70 0.15 62 10 84 74 90.71 0.15 63 10 85 75 90.72 0.14 64 11 14 3 829.78 1.49 65 11 15 4 826.24 0.21 66 11 235 224 59.72 0.96 67 11 236 225 65.47 1.11 68 11 237 226 66.06 0.89 69 12 15 3 836.79 2.73 70 12 16 4 826.23 0.21 71 12 35 23 122.00 22.46 72 12 52 40 117.75 24.39 73 12 89 77 90.66 0.12 74 12 91 79 90.94 0.02 75 13 16 3 827.68 0.52 76 13 17 4 823.52 1.14 77 13 76 63 91.34 0.10 78 13 79 66 91.31 0.10 79 13 80 67 91.95 0.20 80 13 83 70 91.75 0.31 81 14 17 3 827.75 0.48 82 14 18 4 820.93 0.19 83 14 214 200 67.95 0.36 84 14 215 201 68.54 0.22 85 15 18 3 836.94 4.11 86 15 19 4 825.01 1.85 87 15 50 35 170.89 1.53 88 15 52 37 123.62 21.98 89 15 215 200 75.17 0.03 90 15 235 220 79.84 0.54 91 15 238 223 80.38 0.38 92 15 239 224 80.34 0.37 93 15 240 225 79.56 0.24 94 15 241 226 71.76 1.32 95 16 19 3 828.86 0.07 96 16 20 4 820.97 0.18 97 16 26 10 217.87 3.98 98 16 28 12 205.25 2.31 99 16 50 34 187.67 6.49 100 16 72 56 91.33 0.10 101 17 20 3 828.08 3.74 102 17 21 4 820.99 0.20 103 18 21 3 839.23 1.83 104 18 211 193 77.66 0.20 105 18 215 197 80.45 0.33 106 18 241 223 80.38 0.35 107 18 243 225 79.28 0.18 108 19 22 3 840.20 1.35 109 19 23 4 819.82 0.79 110 19 26 7 570.14 13.48 111 19 37 18 205.83 2.02 112 19 39 20 234.46 4.32 113 19 48 29 200.54 3.50 114 19 50 31 194.79 2.94 115 19 241 222 80.45 0.34 116 20 23 3 822.27 2.02 117 20 24 4 812.70 4.33 118 20 72 52 135.28 11.31 119 20 76 56 141.53 6.65 120 21 207 186 74.19 1.08 121 21 210 189 80.41 0.32 122 21 211 190 80.42 0.33 123 22 39 17 244.49 0.53 124 22 40 18 254.58 4.50 125 22 41 19 290.55 9.68 126 22 46 24 307.86 0.47 127 22 48 26 240.75 2.27 128 23 39 16 308.36 0.77 129 24 70 46 160.62 1.15 130 24 71 47 160.36 1.21 131 24 72 48 160.19 1.14 132 24 73 49 160.05 1.12 133 24 76 52 160.05 1.11 134 25 39 14 311.44 0.73 135 25 70 45 160.61 1.15 136 25 71 46 160.38 1.20 137 26 37 11 311.53 0.77 138 26 39 13 311.44 0.73 139 26 68 42 162.40 0.94 140 26 69 43 162.25 0.96 141 26 70 44 160.63 1.14 142 26 72 46 160.60 1.15 143 27 37 10 311.54 0.77 144 27 39 12 311.53 0.77 145 27 67 40 162.63 1.04 146 27 68 41 162.40 0.94 147 27 69 42 162.27 0.96 148 27 123 96 99.73 2.02 149 28 36 8 311.66 0.71 150 28 37 9 311.55 0.77 151 28 50 22 311.44 0.72 152 28 67 39 162.66 1.04 153 28 68 40 162.40 0.94 154 28 70 42 162.40 0.94 155 28 91 63 159.26 1.23 156 28 98 70 158.43 1.23 157 29 35 6 415.75 52.73 158 29 36 7 311.68 0.71 159 29 66 37 162.85 0.98 160 29 67 38 162.82 0.98 161 29 122 93 116.20 0.88 162 29 123 94 112.41 1.02 163 30 34 4 665.71 20.48 164 30 35 5 545.90 12.35 165 30 62 32 163.09 1.00 166 30 65 35 162.94 0.99 167 30 66 36 162.93 0.99 168 30 68 38 162.87 0.97 169 30 91 61 159.55 1.20 170 30 98 68 159.32 1.25 171 31 34 3 818.08 0.53 172 31 36 5 636.26 12.42 173 31 60 29 209.70 0.48 174 31 62 31 165.51 1.01 175 31 65 34 162.94 0.99 176 31 122 91 157.55 1.14 177 32 54 22 311.25 0.65 178 32 60 28 230.71 0.92 179 32 65 33 163.03 0.99 180 33 53 20 311.31 0.68 181 33 54 21 311.25 0.65 182 34 52 18 311.32 0.69 183 34 53 19 311.29 0.69 184 34 54 20 311.12 0.64 185 34 60 26 209.66 0.48 186 35 50 15 311.53 0.77 187 35 51 16 311.34 0.70 188 35 52 17 311.31 0.70 189 36 50 14 311.43 0.73 190 36 51 15 311.31 0.70 191 36 123 87 158.99 1.28 192 36 124 88 158.96 1.28 193 36 125 89 158.25 1.28 194 37 49 12 311.50 0.77 195 37 50 13 311.35 0.72 196 38 47 9 311.65 0.78 197 38 48 10 311.61 0.78 198 38 49 11 311.54 0.76 199 38 125 87 158.98 1.28 200 38 126 88 158.94 1.29 201 39 47 8 311.67 0.79 202 39 48 9 311.64 0.78 203 40 44 4 692.50 30.43 204 40 46 6 415.60 52.70 205 40 47 7 311.67 0.79 206 41 44 3 778.64 22.93 207 41 45 4 692.50 30.43 208 41 46 5 559.25 19.12 209 41 204 163 157.83 1.30 210 45 201 156 157.80 1.29 211 45 204 159 158.06 1.28 212 45 244 199 158.22 1.29 213 45 245 200 157.80 1.30 214 45 246 201 157.75 1.28 215 46 204 158 157.80 1.29 216 46 207 161 158.12 1.32 217 46 208 162 158.16 1.30 218 46 243 197 158.21 1.30 219 46 244 198 157.84 1.29 220 46 245 199 157.78 1.29 221 47 128 81 158.94 1.28 222 47 243 196 158.00 1.33 223 47 244 197 157.83 1.28 224 48 242 194 158.06 1.31 225 48 243 195 157.81 1.29 226 49 125 76 158.97 1.28 227 49 127 78 158.87 1.28 228 49 241 192 158.12 1.28 229 49 242 193 157.82 1.28 230 49 244 195 157.78 1.28 231 50 240 190 158.11 1.29 232 50 241 191 157.83 1.28 233 51 125 74 158.85 1.26 234 51 127 76 158.72 1.31 235 51 239 188 158.09 1.29 236 51 240 189 157.84 1.29 237 51 242 191 157.82 1.28 238 52 56 4 837.93 4.00 239 52 57 5 802.66 11.57 240 52 239 187 157.87 1.29 241 53 56 3 853.58 2.26 242 53 239 186 157.85 1.29 243 54 57 3 837.92 3.99 244 54 60 6 571.22 22.02 245 56 237 181 158.05 1.34 246 56 238 182 158.02 1.34 247 56 239 183 158.02 1.34 248 57 60 3 872.87 4.17 249 62 65 3 845.20 0.59 250 65 122 57 157.05 1.23 251 66 93 27 158.97 1.44 252 66 119 53 157.74 1.06 253 66 120 54 157.68 1.06 254 66 122 56 145.38 2.62 255 67 119 52 157.56 1.14 256 67 120 53 157.01 1.22 257 67 121 54 149.83 0.40 258 67 122 55 117.78 0.08 259 68 98 30 158.18 1.17 260 68 117 49 157.75 1.06 261 68 118 50 157.69 1.08 262 68 119 51 157.00 1.23 263 69 117 48 157.69 1.08 264 69 118 49 157.10 1.26 265 70 98 28 157.92 1.07 266 70 115 45 157.81 1.03 267 70 117 47 157.19 1.11 268 72 75 3 826.47 4.29 269 72 76 4 812.36 1.24 270 73 76 3 850.79 1.64 271 73 77 4 844.60 1.47 272 74 77 3 859.58 0.83 273 75 78 3 834.25 1.38 274 75 79 4 825.14 0.06 275 75 113 38 158.75 1.27 276 75 115 40 158.12 1.15 277 76 79 3 825.07 0.10 278 76 80 4 823.85 0.70 279 77 80 3 836.31 2.12 280 77 81 4 823.84 0.70 281 78 81 3 834.31 1.05 282 78 82 4 818.44 0.49 283 78 113 35 158.90 1.22 284 79 82 3 820.52 1.02 285 79 83 4 810.12 4.67 286 79 89 10 167.39 0.77 287 79 100 21 158.93 1.22 288 79 102 23 159.59 1.20 289 79 113 34 158.91 1.21 290 82 102 20 169.51 3.71 291 82 111 29 169.46 3.74 292 84 87 3 800.12 3.55 293 84 104 20 608.37 88.11 294 86 105 19 608.98 87.83 295 86 106 20 608.98 87.83 296 87 102 15 609.28 87.94 297 87 103 16 609.20 87.94 298 87 104 17 608.99 87.84 299 88 102 14 609.33 87.96 300 88 103 15 609.27 87.94 301 89 100 11 609.44 87.99 302 89 101 12 609.40 87.99 303 89 102 13 609.37 87.98 304 90 99 9 609.57 87.95 305 90 100 10 609.44 87.99 306 90 101 11 609.42 87.98 307 90 103 13 609.43 87.98 308 91 98 7 676.48 54.66 309 91 99 8 609.57 87.95 310 91 100 9 609.45 87.99 311 92 98 6 676.48 54.66 312 92 99 7 609.72 88.03 313 93 97 4 767.74 9.11 314 93 98 5 676.54 54.68 315 93 119 26 584.45 88.86 316 94 97 3 822.10 2.46 317 94 98 4 719.38 33.29 318 94 99 5 719.38 33.29 319 94 119 25 608.38 88.12 320 95 119 24 609.00 87.84 321 96 116 20 609.15 87.90 322 96 117 21 609.14 87.90 323 97 115 18 609.15 87.90 324 97 116 19 609.04 87.86 325 97 117 20 608.76 87.98 326 97 119 22 606.29 87.67 327 98 114 16 609.19 87.89 328 98 115 17 609.12 87.89 329 99 112 13 609.25 87.89 330 99 113 14 609.19 87.88 331 99 114 15 609.10 87.88 332 100 111 11 609.29 87.88 333 100 112 12 609.20 87.87 334 100 113 13 609.18 87.88 335 100 115 15 609.08 87.87 336 101 110 9 609.31 87.86 337 101 111 10 609.24 87.86 338 101 112 11 609.21 87.86 339 102 109 7 609.40 87.83 340 102 110 8 609.27 87.84 341 102 111 9 609.24 87.85 342 102 113 11 609.23 87.86 343 103 109 6 609.38 87.81 344 103 110 7 609.28 87.84 345 104 107 3 844.74 4.03 346 104 108 4 698.59 43.22 347 104 109 5 609.40 87.81 348 127 230 103 302.00 9.85 349 127 242 115 340.04 9.16 350 127 244 117 229.46 2.79 351 128 244 116 301.96 9.85 352 130 227 97 360.31 0.47 353 130 228 98 361.54 1.07 354 130 244 114 365.25 1.03 355 131 227 96 408.58 19.90 356 132 222 90 417.21 15.64 357 132 223 91 408.62 19.92 358 132 224 92 416.70 15.85 359 132 227 95 416.70 15.85 360 132 228 96 408.60 19.90 361 132 230 98 408.82 19.86 362 132 244 112 417.22 15.65 363 133 193 60 429.26 20.38 364 133 194 61 423.34 17.46 365 133 223 90 418.77 15.33 366 133 224 91 418.39 15.41 367 134 161 27 425.66 18.57 368 134 166 32 433.42 20.66 369 134 191 57 434.63 20.59 370 134 192 58 433.89 20.71 371 134 194 60 433.10 20.87 372 134 196 62 422.96 17.41 373 134 222 88 429.36 20.46 374 134 223 89 422.82 17.36 375 135 190 55 450.72 14.47 376 135 191 56 437.19 19.41 377 135 220 85 434.83 20.55 378 135 221 86 434.29 20.48 379 135 222 87 430.38 19.97 380 136 159 23 434.78 20.59 381 136 166 30 437.16 19.39 382 136 181 45 459.89 16.25 383 136 187 51 460.13 16.17 384 136 189 53 459.82 16.19 385 136 190 54 445.56 17.09 386 136 220 84 437.05 19.43 387 136 221 85 434.44 20.46 388 136 223 87 434.46 20.47 389 137 187 50 460.16 16.18 390 137 188 51 460.09 16.22 391 137 189 52 459.88 16.16 392 137 218 81 460.18 16.20 393 137 219 82 459.10 16.23 394 137 220 83 437.06 19.43 395 138 142 4 821.39 0.46 396 138 143 5 811.10 0.79 397 138 187 49 460.17 16.18 398 138 188 50 460.23 16.18 399 138 218 80 460.38 16.12 400 138 219 81 460.14 16.18 401 139 142 3 843.01 3.84 402 139 143 4 821.38 0.46 403 139 188 49 471.89 21.63 404 139 219 80 460.36 16.12 405 140 143 3 831.16 3.41 406 140 144 4 827.70 2.69 407 140 212 72 470.52 17.96 408 140 213 73 471.29 18.33 409 140 215 75 475.19 19.99 410 140 219 79 465.57 18.68 411 141 144 3 835.62 1.18 412 141 145 4 827.70 2.70 413 142 145 3 832.55 0.35 414 142 146 4 823.80 4.65 415 142 181 39 501.82 31.81 416 142 186 44 501.81 31.82 417 143 146 3 826.70 3.28 418 143 147 4 823.79 4.65 419 143 205 62 475.41 20.07 420 143 209 66 475.45 20.05 421 143 212 69 476.74 19.44 422 143 219 76 475.67 19.96 423 143 221 78 475.41 20.08 424 143 231 88 475.40 20.07 425 144 147 3 830.97 0.95 426 144 148 4 815.81 0.66 427 144 209 65 475.59 20.00 428 144 210 66 477.62 19.82 429 144 213 69 477.66 19.79 430 145 148 3 823.88 2.60 431 145 149 4 821.76 3.63 432 146 149 3 824.25 2.39 433 146 150 4 821.59 3.72 434 146 157 11 579.19 20.50 435 146 168 22 511.10 35.69 436 146 181 35 510.44 27.64 437 146 205 59 479.32 19.84 438 147 150 3 840.62 5.79 439 147 151 4 833.26 2.10 440 147 205 58 479.31 19.85 441 147 206 59 479.31 19.85 442 147 209 62 479.29 19.85 443 148 151 3 836.21 1.52 444 148 152 4 833.26 2.10 445 148 206 58 481.74 21.05 446 149 152 3 835.24 3.09 447 149 153 4 833.27 2.11 448 149 177 28 579.24 20.52 449 150 153 3 835.38 1.10 450 150 154 4 803.77 1.82 451 150 157 7 586.04 17.12 452 150 168 18 567.23 14.53 453 150 170 20 587.98 16.12 454 150 177 27 588.00 16.14 455 150 202 52 482.00 21.10 456 151 154 3 836.43 0.58 457 151 202 51 482.06 21.13 458 151 203 52 482.03 21.10 459 151 206 55 482.01 21.11 460 153 170 17 670.16 27.07 461 153 171 18 670.16 27.08 462 153 175 22 691.75 16.30 463 153 176 23 668.04 28.12 464 153 177 24 633.41 10.80 465 154 171 17 706.89 16.82 466 154 202 48 502.03 18.38 467 155 172 17 715.66 13.85 468 155 201 46 502.08 18.39 469 155 202 47 502.03 18.38 470 155 248 93 473.14 18.34 471 156 170 14 715.69 13.88 472 156 172 16 715.70 13.83 473 156 199 43 505.69 18.53 474 156 200 44 505.37 18.62 475 156 202 46 502.85 18.77 476 156 248 92 473.11 18.36 477 157 168 11 715.96 14.01 478 157 169 12 715.93 13.98 479 157 199 42 506.38 18.87 480 157 200 43 505.60 18.51 481 157 202 45 503.07 17.89 482 157 205 48 504.31 18.23 483 158 168 10 715.96 14.01 484 158 169 11 715.94 13.98 485 158 171 13 716.11 13.89 486 158 172 14 716.10 13.88 487 158 198 40 506.90 18.77 488 158 199 41 506.81 18.77 489 159 166 7 716.00 14.03 490 159 167 8 715.97 14.01 491 159 168 9 715.97 14.01 492 159 197 38 507.23 18.84 493 159 198 39 507.01 18.77 494 159 200 41 506.97 18.79 495 159 223 64 506.78 18.96 496 159 229 70 506.63 18.89 497 160 166 6 715.99 14.03 498 160 167 7 715.97 14.01 499 160 196 36 507.36 18.80 500 160 197 37 507.24 18.85 501 160 223 63 507.20 18.85 502 161 165 4 738.75 25.22 503 161 192 31 542.40 19.62 504 161 195 34 507.44 18.84 505 161 196 35 507.43 18.83 506 161 223 62 507.30 18.86 507 162 165 3 799.51 3.90 508 162 167 5 716.18 13.94 509 162 180 18 715.85 13.78 510 164 182 18 715.66 13.85 511 164 190 26 610.60 22.04 512 164 192 28 579.58 20.42 513 165 180 15 715.88 13.77 514 165 181 16 715.82 13.77 515 165 182 17 715.62 13.83 516 165 192 27 585.61 23.45 517 166 180 14 715.85 13.75 518 166 181 15 715.79 13.76 519 166 187 21 618.05 23.59 520 166 190 24 597.37 17.57 521 166 192 26 553.36 23.78 522 166 223 57 507.22 18.87 523 167 178 11 716.03 13.71 524 167 179 12 715.94 13.71 525 167 180 13 715.89 13.71 526 168 177 9 716.10 13.68 527 168 178 10 715.98 13.68 528 168 179 11 715.94 13.70 529 168 181 13 715.90 13.70 530 169 177 8 716.09 13.68 531 169 178 9 716.03 13.70 532 170 176 6 716.16 13.67 533 170 177 7 716.12 13.67 534 171 175 4 735.07 4.57 535 171 176 5 716.18 13.66 536 172 176 4 808.64 3.38 537 173 176 3 923.41 2.53 538 181 185 4 797.28 0.69 539 181 186 5 796.34 0.23 540 181 187 6 740.44 27.71 541 182 185 3 821.80 0.84 542 182 186 4 809.72 6.88 543 182 187 5 740.45 27.72 544 182 190 8 610.97 21.98 545 183 187 4 796.38 0.24 546 183 188 5 769.93 12.98 547 183 189 6 677.38 6.08 548 183 190 7 676.86 6.17 549 184 187 3 819.69 1.90 550 187 190 3 866.38 2.02 551 188 218 30 485.07 19.52 552 196 223 27 514.89 18.30 553 196 224 28 514.79 18.26 554 196 225 29 515.41 18.39 555 197 225 28 515.80 18.43 556 197 226 29 516.02 18.50 557 198 224 26 516.04 18.51 558 198 226 28 516.19 18.59 559 198 227 29 516.22 18.57 560 198 247 49 476.14 19.69 561 199 247 48 474.80 19.13 562 199 248 49 473.15 18.34 563 200 223 23 518.29 19.10 564 200 227 27 518.59 19.10 565 200 228 28 518.12 19.03 566 200 229 29 517.95 19.05 567 200 245 45 477.87 19.77 568 200 246 46 477.46 19.60 569 200 247 47 475.12 19.33 570 200 248 48 473.11 18.36 571 201 204 3 819.61 4.29 572 201 205 4 768.34 19.07 573 201 248 47 473.05 18.39 574 202 205 3 836.56 0.76 575 202 206 4 808.24 5.62 576 203 206 3 828.41 3.31 577 203 207 4 778.41 20.55 578 204 207 3 834.33 0.35 579 204 208 4 803.61 7.96 580 204 245 41 477.87 19.78 581 205 208 3 827.97 0.45 582 205 209 4 823.30 1.88 583 205 229 24 519.21 19.14 584 205 231 26 519.06 19.22 585 205 245 40 477.81 19.79 586 206 209 3 839.16 3.17 587 206 210 4 810.71 4.38 588 207 210 3 840.76 0.81 589 208 211 3 850.21 0.36 590 208 212 4 840.07 1.16 591 208 231 23 519.09 19.22 592 208 243 35 479.57 19.62 593 208 245 37 479.04 19.47 594 209 212 3 842.24 4.35 595 209 231 22 519.29 19.12 596 210 213 3 855.19 0.03 597 211 214 3 871.29 0.12 598 211 215 4 849.98 0.48 599 212 215 3 847.52 0.74 600 212 219 7 522.00 17.88 601 212 231 19 519.61 19.08 602 212 233 21 519.27 19.15 603 212 241 29 481.49 19.91 604 212 243 31 481.17 20.03 605 215 233 18 519.56 19.11 606 215 235 20 518.98 19.25 607 215 241 26 482.19 20.09 608 216 236 20 519.08 19.25 609 217 233 16 520.39 19.22 610 217 234 17 519.62 19.07 611 217 236 19 519.40 19.07 612 218 233 15 520.42 19.21 613 219 231 12 522.70 18.48 614 219 232 13 522.13 18.65 615 219 233 14 520.45 19.23 616 220 230 10 523.31 18.65 617 220 231 11 522.71 18.48 618 220 232 12 522.15 18.64 619 221 229 8 523.41 18.67 620 221 230 9 523.26 18.66 621 221 231 10 523.16 18.69 622 222 229 7 523.46 18.66 623 222 230 8 523.29 18.66 624 223 228 5 524.75 18.32 625 223 229 6 523.47 18.66 626 224 227 3 807.21 0.69 627 226 247 21 477.55 19.82 628 227 247 20 477.86 19.67 629 228 244 16 479.56 19.66 630 228 245 17 479.37 19.64 631 228 246 18 478.62 19.98 632 228 247 19 478.01 19.75 633 229 243 14 480.38 19.64 634 229 244 15 479.56 19.66 635 229 245 16 479.37 19.64 636 230 242 12 480.46 19.66 637 230 243 13 480.38 19.64 638 230 244 14 479.66 19.71 639 231 242 11 480.47 19.66 640 231 243 12 480.38 19.63 641 231 245 14 480.35 19.64 642 232 240 8 525.75 18.74 643 232 241 9 525.49 18.75 644 232 242 10 524.78 18.60 645 233 240 7 525.73 18.75 646 233 241 8 525.67 18.77 647 234 239 5 537.57 23.16 648 234 240 6 526.72 18.25 649 235 238 3 821.18 2.20 650 235 239 4 572.52 5.70