BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 20 18 30.87 0.52 2 2 21 19 28.69 1.20 3 2 24 22 28.66 1.21 4 2 50 48 32.13 0.41 5 2 51 49 38.09 0.10 6 3 51 48 38.09 0.10 7 4 9 5 797.84 1.00 8 4 13 9 52.22 1.36 9 4 14 10 41.52 0.23 10 4 17 13 39.27 0.26 11 4 51 47 40.63 0.42 12 4 52 48 40.64 0.39 13 4 53 49 51.83 0.28 14 4 129 125 60.48 0.18 15 4 132 128 60.36 0.22 16 5 9 4 834.22 5.86 17 5 53 48 57.62 0.75 18 5 57 52 60.55 0.21 19 6 9 3 807.10 5.62 20 6 53 47 60.37 0.21 21 6 54 48 60.48 0.17 22 6 55 49 61.62 0.01 23 6 129 123 60.48 0.18 24 6 132 126 60.48 0.18 25 6 133 127 60.49 0.18 26 6 136 130 60.48 0.18 27 7 55 48 64.45 1.18 28 9 13 4 819.84 0.47 29 9 129 120 80.76 0.07 30 9 175 166 85.54 0.18 31 10 13 3 829.61 0.69 32 10 14 4 826.88 0.68 33 10 129 119 86.36 0.23 34 10 175 165 87.48 0.34 35 11 14 3 848.10 4.70 36 11 15 4 832.11 3.29 37 11 172 161 80.77 0.07 38 11 173 162 84.34 0.06 39 11 175 164 89.42 0.73 40 12 15 3 832.46 3.12 41 12 16 4 839.00 0.15 42 13 16 3 839.30 0.30 43 13 17 4 839.00 0.15 44 14 17 3 831.10 4.10 45 14 18 4 833.93 2.69 46 14 129 115 98.91 0.28 47 14 157 143 94.48 0.42 48 14 161 147 91.67 1.84 49 14 168 154 91.67 1.84 50 14 171 157 98.39 0.14 51 14 172 158 96.38 0.51 52 15 18 3 838.88 0.21 53 15 19 4 828.37 0.10 54 15 168 153 94.83 0.27 55 16 19 3 838.12 0.51 56 16 20 4 828.37 0.10 57 17 20 3 845.81 0.99 58 17 21 4 828.35 0.11 59 17 50 33 105.71 0.31 60 17 52 35 114.09 0.07 61 17 158 141 99.09 0.28 62 18 21 3 834.72 3.07 63 18 22 4 830.04 0.89 64 18 157 139 104.90 0.04 65 18 158 140 99.34 0.40 66 18 161 143 98.94 0.26 67 18 162 144 98.55 0.15 68 18 168 150 100.65 0.26 69 19 22 3 832.33 0.25 70 19 23 4 824.60 1.79 71 20 23 3 828.17 0.06 72 20 24 4 824.64 1.82 73 21 24 3 852.32 0.46 74 21 25 4 833.13 2.43 75 21 50 29 119.60 0.62 76 21 64 43 117.64 0.04 77 21 158 137 116.83 0.33 78 21 162 141 112.38 0.35 79 22 25 3 842.98 0.61 80 22 161 139 117.73 0.04 81 22 162 140 114.14 0.54 82 22 165 143 117.71 0.03 83 22 168 146 118.08 0.12 84 22 238 216 107.70 0.63 85 22 239 217 105.75 0.29 86 25 37 12 121.28 0.94 87 25 64 39 121.17 0.94 88 25 66 41 120.52 0.66 89 25 69 44 120.00 0.60 90 25 239 214 119.98 0.59 91 26 37 11 121.48 0.90 92 26 38 12 121.61 0.92 93 26 41 15 121.78 0.90 94 26 66 40 121.37 0.84 95 27 35 8 121.45 0.90 96 27 36 9 121.47 0.94 97 27 37 10 121.49 0.90 98 27 38 11 121.62 0.92 99 28 36 8 121.64 0.93 100 28 37 9 121.64 0.92 101 28 38 10 121.64 0.92 102 28 242 214 118.38 0.17 103 29 35 6 197.13 38.49 104 29 36 7 122.31 1.12 105 30 33 3 850.21 1.28 106 30 34 4 540.69 114.50 107 30 35 5 197.13 38.49 108 30 36 6 122.80 1.34 109 31 34 3 821.49 0.10 110 31 36 5 290.13 8.01 111 31 200 169 122.83 1.34 112 31 203 172 144.30 6.00 113 31 240 209 128.84 1.64 114 32 199 167 146.37 5.29 115 32 202 170 146.47 5.33 116 32 203 171 146.47 5.33 117 32 207 175 146.48 5.34 118 32 228 196 146.48 5.33 119 34 199 165 149.86 3.66 120 34 233 199 149.88 3.67 121 34 237 203 150.06 3.52 122 34 238 204 150.04 3.52 123 34 239 205 150.03 3.50 124 34 240 206 149.98 3.52 125 35 238 203 150.13 3.52 126 35 239 204 150.06 3.50 127 35 240 205 150.05 3.50 128 36 200 164 150.08 3.56 129 36 240 204 150.06 3.50 130 36 242 206 149.72 3.37 131 37 66 29 361.00 101.98 132 37 67 30 378.87 110.91 133 37 68 31 379.03 110.98 134 37 69 32 379.03 110.98 135 37 192 155 150.28 3.51 136 37 239 202 150.22 3.49 137 37 240 203 150.12 3.51 138 37 241 204 150.10 3.50 139 37 242 205 150.01 3.50 140 38 41 3 912.17 0.34 141 38 67 29 575.83 12.59 142 38 242 204 150.09 3.51 143 39 67 28 611.18 2.27 144 39 242 203 150.09 3.52 145 39 244 205 148.09 4.35 146 39 245 206 83.35 21.93 147 40 67 27 622.26 7.71 148 41 45 4 798.48 2.91 149 41 46 5 755.23 6.81 150 41 66 25 690.78 16.77 151 41 67 26 690.79 16.78 152 42 45 3 843.38 2.98 153 42 46 4 792.76 0.42 154 43 46 3 838.94 0.76 155 43 47 4 843.32 2.94 156 43 94 51 622.27 7.69 157 44 94 50 631.05 12.05 158 45 66 21 754.37 6.80 159 46 65 19 754.35 6.78 160 46 66 20 754.37 6.80 161 46 67 21 754.35 6.81 162 46 90 44 685.49 0.54 163 47 64 17 754.38 6.77 164 47 65 18 754.36 6.78 165 47 66 19 754.38 6.79 166 47 72 25 753.71 7.06 167 47 90 43 687.08 0.23 168 47 91 44 685.44 0.54 169 47 94 47 685.04 0.40 170 47 95 48 683.95 0.37 171 48 62 14 754.68 6.85 172 48 63 15 754.57 6.86 173 48 64 16 754.54 6.85 174 48 66 18 754.42 6.81 175 49 62 13 754.68 6.85 176 49 63 14 754.62 6.83 177 49 95 46 687.18 0.19 178 49 96 47 687.02 0.21 179 50 61 11 754.73 6.85 180 50 62 12 754.68 6.85 181 50 64 14 754.66 6.86 182 50 158 108 456.66 4.79 183 51 59 8 755.12 6.96 184 51 60 9 754.90 6.86 185 51 61 10 754.73 6.85 186 52 59 7 755.12 6.96 187 52 60 8 755.00 6.91 188 52 62 10 754.91 6.86 189 52 132 80 540.10 3.21 190 52 136 84 541.16 2.46 191 52 154 102 505.87 0.58 192 52 158 106 461.90 2.75 193 53 57 4 762.03 3.57 194 53 58 5 755.21 6.94 195 53 59 6 755.12 6.96 196 53 136 83 541.95 2.29 197 54 57 3 827.78 6.83 198 54 58 4 755.29 6.94 199 54 133 79 541.36 2.58 200 54 136 82 541.95 2.29 201 54 137 83 541.95 2.28 202 55 130 75 542.02 2.31 203 55 133 78 541.97 2.29 204 58 136 78 542.03 2.25 205 58 140 82 541.79 2.06 206 58 141 83 541.81 2.04 207 59 140 81 541.75 2.06 208 60 74 14 753.75 7.04 209 60 75 15 753.72 7.04 210 60 76 16 753.38 7.12 211 60 136 76 541.49 2.12 212 60 139 79 541.68 2.08 213 60 140 80 541.59 2.04 214 60 154 94 508.70 0.82 215 61 74 13 753.74 7.05 216 61 75 14 753.72 7.05 217 61 96 35 687.89 0.05 218 62 71 9 754.21 6.92 219 62 73 11 753.77 7.08 220 62 74 12 753.72 7.05 221 62 96 34 687.87 0.04 222 62 154 92 504.81 0.76 223 63 71 8 754.13 6.95 224 63 72 9 753.83 7.08 225 63 73 10 753.73 7.05 226 63 95 32 687.66 0.04 227 63 96 33 687.36 0.28 228 64 69 5 754.68 6.71 229 64 70 6 754.12 6.98 230 64 71 7 753.85 7.09 231 64 72 8 753.83 7.11 232 65 68 3 854.98 3.21 233 65 69 4 754.69 6.71 234 65 70 5 754.67 6.71 235 65 72 7 753.84 7.11 236 67 245 178 142.47 7.62 237 68 163 95 443.50 0.46 238 68 192 124 294.16 0.91 239 68 241 173 156.38 1.44 240 68 245 177 142.44 7.61 241 69 159 90 445.61 0.62 242 69 162 93 443.56 0.47 243 69 163 94 443.48 0.43 244 69 192 123 294.45 1.05 245 69 239 170 183.67 5.03 246 70 163 93 444.73 1.06 247 71 83 12 688.38 0.14 248 71 154 83 503.43 1.43 249 71 155 84 492.43 2.35 250 71 158 87 445.91 0.58 251 71 159 88 445.73 0.66 252 72 82 10 688.37 0.15 253 72 83 11 688.34 0.12 254 72 87 15 688.11 0.06 255 72 88 16 687.99 0.03 256 72 91 19 687.94 0.04 257 73 81 8 688.40 0.14 258 73 82 9 688.37 0.15 259 73 96 23 687.90 0.04 260 74 80 6 688.49 0.14 261 74 81 7 688.44 0.14 262 74 96 22 687.91 0.04 263 74 139 65 541.56 2.04 264 74 142 68 541.48 1.97 265 74 151 77 534.44 0.92 266 75 78 3 918.10 4.12 267 75 79 4 790.76 6.92 268 75 80 5 688.49 0.14 269 75 96 21 687.91 0.04 270 75 98 23 687.88 0.06 271 75 99 24 686.29 0.18 272 76 79 3 860.05 0.81 273 76 81 5 803.33 3.47 274 76 139 63 541.69 2.07 275 76 140 64 541.60 2.02 276 78 99 21 687.52 0.00 277 78 100 22 687.07 0.22 278 78 101 23 684.95 0.29 279 79 99 20 687.47 0.02 280 79 101 22 684.79 0.27 281 79 103 24 670.76 1.52 282 80 96 16 687.92 0.04 283 80 99 19 686.66 0.36 284 80 101 21 684.79 0.27 285 80 102 22 667.00 3.40 286 80 103 23 662.69 2.23 287 81 103 22 621.79 16.97 288 81 105 24 543.65 3.14 289 81 142 61 541.27 1.86 290 82 88 6 688.38 0.04 291 82 91 9 688.37 0.04 292 82 92 10 688.37 0.04 293 82 95 13 688.28 0.06 294 82 96 14 688.00 0.01 295 82 103 21 641.62 7.06 296 82 104 22 543.66 3.13 297 82 105 23 543.63 3.13 298 83 88 5 714.91 13.30 299 83 105 22 543.63 3.12 300 83 107 24 542.92 3.08 301 83 108 25 542.89 3.06 302 83 109 26 542.84 3.05 303 83 114 31 542.85 3.04 304 83 151 68 537.94 0.59 305 83 155 72 505.94 0.59 306 84 104 20 548.69 5.63 307 84 105 21 543.66 3.13 308 84 107 23 543.00 3.12 309 84 108 24 542.92 3.08 310 85 88 3 859.82 1.06 311 85 89 4 824.82 4.24 312 85 108 23 542.91 3.08 313 85 109 24 542.88 3.08 314 86 89 3 843.55 0.41 315 86 90 4 837.73 2.22 316 87 90 3 852.31 2.71 317 87 91 4 846.57 2.06 318 88 91 3 846.57 2.06 319 88 92 4 850.00 0.35 320 89 92 3 850.01 0.35 321 89 93 4 850.06 0.34 322 90 93 3 858.95 1.15 323 90 94 4 850.06 0.34 324 91 94 3 853.77 1.44 325 91 95 4 850.05 0.34 326 92 95 3 844.82 2.28 327 93 97 4 844.47 2.11 328 94 97 3 911.97 2.83 329 96 99 3 840.80 0.55 330 96 102 6 768.35 4.92 331 97 102 5 812.51 3.17 332 99 102 3 882.39 3.83 333 105 142 37 542.06 1.88 334 105 151 46 541.31 1.77 335 106 142 36 542.18 1.84 336 107 113 6 549.29 4.96 337 107 114 7 544.12 2.45 338 107 117 10 544.07 2.40 339 107 147 40 542.37 1.67 340 107 148 41 542.19 1.58 341 107 151 44 542.10 1.53 342 108 113 5 750.39 23.46 343 109 114 5 801.99 8.15 344 110 113 3 843.37 0.59 345 110 114 4 819.90 4.80 346 111 114 3 836.39 2.89 347 111 115 4 819.90 4.80 348 111 122 11 543.24 1.89 349 111 152 41 542.44 1.51 350 111 155 44 542.07 1.34 351 111 156 45 541.44 1.03 352 111 160 49 499.72 0.06 353 111 189 78 313.17 5.33 354 112 115 3 832.62 6.12 355 112 116 4 815.74 1.36 356 113 116 3 843.01 6.12 357 113 117 4 814.38 2.04 358 114 117 3 832.50 6.07 359 114 118 4 815.05 2.40 360 114 148 34 542.66 1.51 361 114 151 37 542.54 1.49 362 114 152 38 542.52 1.48 363 114 155 41 541.54 0.98 364 115 118 3 835.46 7.54 365 115 119 4 819.73 5.38 366 115 120 5 698.59 1.77 367 115 121 6 544.18 2.22 368 115 122 7 543.83 2.04 369 115 152 37 542.55 1.47 370 116 119 3 822.54 8.54 371 117 148 31 542.74 1.48 372 118 148 30 542.74 1.48 373 118 149 31 542.71 1.46 374 118 152 34 542.66 1.44 375 120 125 5 707.62 3.13 376 120 152 32 542.67 1.41 377 121 124 3 852.36 3.06 378 121 125 4 828.13 1.51 379 122 125 3 831.08 5.45 380 122 152 30 542.37 1.24 381 122 156 34 541.54 0.86 382 122 181 59 425.32 0.55 383 122 186 64 389.20 0.01 384 122 189 67 346.81 2.44 385 122 190 68 338.28 1.83 386 123 180 57 426.47 0.90 387 123 181 58 426.42 0.88 388 123 183 60 422.88 1.59 389 123 186 63 391.12 0.55 390 124 180 56 426.75 0.78 391 125 149 24 543.11 0.83 392 125 152 27 543.08 0.83 393 125 153 28 542.41 0.49 394 125 180 55 426.50 0.90 395 125 181 56 426.31 0.94 396 126 131 5 781.39 1.08 397 126 153 27 542.45 0.48 398 126 178 52 427.12 0.95 399 126 179 53 427.06 0.92 400 126 180 54 426.44 0.87 401 127 131 4 810.00 4.31 402 127 153 26 542.25 0.37 403 127 156 29 541.30 0.09 404 127 171 44 441.07 0.62 405 127 178 51 427.12 0.96 406 127 179 52 427.06 0.93 407 127 181 54 424.00 1.00 408 128 131 3 830.51 5.15 409 128 132 4 829.77 5.52 410 128 171 43 440.50 0.36 411 128 174 46 437.97 0.70 412 128 178 50 427.13 0.96 413 128 179 51 427.12 0.96 414 129 132 3 829.77 5.52 415 129 133 4 829.78 5.51 416 129 171 42 440.49 0.37 417 129 172 43 440.29 0.39 418 129 174 45 438.45 0.95 419 129 175 46 434.31 0.13 420 130 133 3 837.29 1.76 421 130 134 4 829.78 5.51 422 130 174 44 439.30 0.53 423 130 175 45 436.94 1.17 424 130 176 46 433.40 0.28 425 130 177 47 428.85 0.20 426 130 178 48 427.14 0.95 427 131 134 3 842.91 1.06 428 131 135 4 829.77 5.51 429 131 153 22 541.71 0.04 430 131 178 47 427.13 0.95 431 132 135 3 835.96 2.39 432 132 136 4 829.75 5.50 433 132 153 21 541.38 0.21 434 132 154 22 540.86 0.47 435 132 157 25 522.96 9.40 436 133 136 3 843.18 0.61 437 133 137 4 837.24 1.72 438 134 137 3 843.63 1.48 439 134 138 4 829.53 5.54 440 135 138 3 838.49 1.07 441 135 139 4 818.21 0.12 442 135 150 15 541.88 0.03 443 135 153 18 540.85 0.48 444 136 139 3 841.24 2.46 445 136 140 4 821.91 1.74 446 137 140 3 837.37 4.38 447 137 141 4 822.25 1.57 448 138 141 3 846.54 1.09 449 138 142 4 802.90 1.21 450 138 143 5 706.31 29.54 451 138 146 8 543.32 0.61 452 138 147 9 542.98 0.46 453 138 150 12 542.20 0.14 454 139 142 3 803.53 2.27 455 139 147 8 542.28 0.09 456 139 150 11 542.21 0.12 457 139 151 12 541.95 0.01 458 139 154 15 526.27 7.83 459 142 147 5 629.83 39.80 460 143 146 3 825.14 1.95 461 143 147 4 734.87 12.71 462 144 147 3 827.06 2.96 463 146 149 3 852.47 1.07 464 146 150 4 826.97 0.77 465 147 150 3 833.14 2.69 466 147 151 4 812.60 0.10 467 148 151 3 837.10 0.44 468 148 152 4 824.51 5.86 469 149 152 3 834.09 0.89 470 149 153 4 824.28 0.29 471 150 153 3 834.09 0.88 472 150 154 4 824.97 3.68 473 151 154 3 839.48 0.37 474 151 155 4 811.31 3.16 475 152 155 3 831.64 1.06 476 152 156 4 828.89 5.63 477 153 156 3 842.15 0.98 478 153 157 4 823.50 8.34 479 154 157 3 850.88 6.59 480 154 158 4 838.02 1.07 481 155 158 3 860.14 9.98 482 155 159 4 809.00 2.12 483 155 160 5 764.71 11.33 484 156 160 4 719.01 33.63 485 156 161 5 757.88 14.21 486 156 167 11 441.48 0.58 487 156 171 15 441.04 0.64 488 156 189 33 304.04 1.52 489 157 161 4 814.51 4.73 490 157 171 14 440.75 0.58 491 158 161 3 838.14 0.30 492 158 162 4 818.56 2.70 493 159 162 3 840.25 0.75 494 159 163 4 820.61 3.73 495 160 163 3 830.12 1.02 496 160 164 4 818.10 5.59 497 160 167 7 495.47 27.57 498 160 189 29 300.95 0.02 499 161 164 3 837.10 3.91 500 161 167 6 441.47 0.58 501 161 168 7 440.97 0.58 502 162 238 76 188.02 6.88 503 162 239 77 187.55 6.97 504 163 192 29 295.53 1.56 505 163 237 74 188.76 7.24 506 163 238 75 188.02 6.87 507 163 239 76 187.55 6.97 508 164 167 3 847.12 4.13 509 164 168 4 824.75 1.44 510 164 188 24 312.17 5.56 511 164 189 25 304.12 1.54 512 164 192 28 296.18 1.73 513 164 195 31 295.68 1.55 514 164 232 68 189.25 7.09 515 164 236 72 188.88 7.21 516 164 237 73 188.81 7.27 517 165 168 3 834.59 2.14 518 165 169 4 828.72 1.89 519 165 236 71 188.93 7.24 520 165 238 73 188.83 7.27 521 166 169 3 838.42 0.21 522 166 170 4 828.77 1.86 523 166 184 18 348.29 3.33 524 166 185 19 348.26 3.34 525 166 188 22 346.63 2.54 526 166 236 70 188.94 7.24 527 167 170 3 844.90 2.35 528 167 171 4 829.28 1.60 529 167 185 18 348.20 3.33 530 167 188 21 313.86 5.14 531 167 189 22 313.85 5.13 532 168 171 3 856.36 0.49 533 168 172 4 841.03 4.28 534 169 172 3 841.58 4.01 535 169 173 4 829.26 1.60 536 170 173 3 849.33 0.53 537 170 174 4 844.97 2.32 538 170 179 9 427.07 0.97 539 170 185 15 378.13 5.89 540 171 174 3 850.87 4.27 541 171 179 8 427.11 0.96 542 172 175 3 862.01 1.30 543 174 177 3 830.30 1.01 544 174 178 4 766.47 21.59 545 174 179 5 670.84 52.65 546 179 185 6 824.95 2.10 547 181 185 4 819.82 0.52 548 181 186 5 819.01 0.12 549 182 185 3 848.83 3.04 550 182 186 4 837.89 8.52 551 183 186 3 842.80 6.07 552 183 187 4 819.87 0.49 553 183 216 33 258.63 3.29 554 183 220 37 258.52 3.33 555 184 187 3 844.63 5.15 556 184 188 4 819.87 0.49 557 184 216 32 258.99 3.46 558 184 217 33 259.02 3.45 559 184 218 34 259.04 3.46 560 185 188 3 837.74 2.58 561 185 189 4 829.53 1.52 562 186 189 3 841.80 6.44 563 186 190 4 829.54 1.52 564 187 190 3 833.86 0.64 565 187 191 4 829.54 1.52 566 187 215 28 259.00 3.46 567 187 216 29 259.01 3.46 568 188 191 3 841.64 3.43 569 188 192 4 824.46 1.02 570 188 215 27 260.20 2.89 571 188 232 44 203.81 0.18 572 188 236 48 203.81 0.18 573 189 192 3 838.64 2.75 574 191 194 3 836.71 3.60 575 191 195 4 814.62 3.82 576 191 213 22 262.01 2.44 577 191 215 24 261.64 2.31 578 192 195 3 837.82 5.26 579 192 196 4 824.94 1.06 580 192 237 45 203.78 0.20 581 192 239 47 203.69 0.17 582 192 240 48 202.43 0.35 583 192 241 49 202.22 0.30 584 193 196 3 847.99 3.56 585 193 197 4 825.31 0.94 586 193 241 48 202.09 0.36 587 194 197 3 831.79 2.30 588 194 198 4 827.20 0.01 589 194 213 19 262.62 2.73 590 195 198 3 835.35 4.08 591 195 199 4 827.22 0.02 592 195 209 14 265.10 2.49 593 195 213 18 262.95 2.57 594 195 225 30 262.61 2.73 595 195 232 37 204.62 0.16 596 195 237 42 203.79 0.20 597 196 199 3 845.54 1.39 598 196 200 4 827.21 0.02 599 196 237 41 204.08 0.34 600 196 240 44 202.66 0.46 601 196 241 45 202.43 0.34 602 197 200 3 845.17 4.98 603 197 201 4 828.25 0.61 604 197 243 46 201.05 0.62 605 198 201 3 845.28 4.92 606 198 202 4 827.11 0.07 607 198 209 11 265.10 2.49 608 198 210 12 265.10 2.49 609 198 211 13 265.04 2.50 610 198 213 15 264.14 2.67 611 199 202 3 841.05 7.04 612 199 203 4 828.88 1.05 613 199 209 10 265.12 2.49 614 199 233 34 206.24 0.65 615 199 237 38 204.30 0.31 616 199 240 41 203.75 0.18 617 200 203 3 847.94 3.66 618 200 240 40 203.76 0.18 619 200 241 41 203.72 0.16 620 200 242 42 203.55 0.24 621 200 243 43 202.91 0.23 622 201 204 3 842.88 6.19 623 201 205 4 827.18 1.91 624 201 210 9 265.15 2.48 625 201 243 42 202.95 0.21 626 202 205 3 827.18 1.91 627 202 206 4 813.45 8.77 628 202 207 5 428.19 65.82 629 202 208 6 265.34 2.39 630 202 209 7 265.17 2.48 631 202 228 26 262.54 2.70 632 205 208 3 850.04 6.88 633 207 227 20 262.58 2.71 634 207 228 21 262.56 2.71 635 208 226 18 262.61 2.73 636 208 227 19 262.54 2.71 637 209 213 4 859.45 2.73 638 209 225 16 262.66 2.75 639 209 226 17 262.55 2.75 640 209 227 18 262.50 2.73 641 209 233 24 228.14 3.71 642 212 224 12 262.91 2.71 643 212 225 13 262.88 2.71 644 213 224 11 263.01 2.75 645 213 225 12 262.88 2.71 646 214 222 8 264.23 3.26 647 214 223 9 263.10 2.72 648 214 224 10 263.01 2.75 649 215 222 7 264.38 3.32 650 215 223 8 264.20 3.27 651 215 225 10 263.10 2.73 652 215 232 17 207.94 1.71 653 216 220 4 429.00 65.70 654 216 221 5 265.68 2.98 655 216 222 6 265.57 3.02 656 217 220 3 814.80 8.43 657 217 221 4 428.82 65.62 658 217 223 6 265.61 3.00 659 225 230 5 308.78 0.58 660 225 232 7 311.92 1.82 661 227 230 3 864.12 0.64 662 227 233 6 669.75 56.67 663 228 233 5 552.15 115.47 664 228 234 6 320.56 0.32 665 229 234 5 669.93 58.12 666 230 233 3 830.56 0.92 667 230 234 4 819.81 2.29 668 231 234 3 833.36 3.25 669 231 235 4 799.16 8.02 670 231 236 5 684.44 65.38 671 232 236 4 554.66 115.78 672 232 237 5 554.63 115.78 673 233 237 4 669.39 58.41 674 237 240 3 925.95 3.14 675 241 245 4 825.45 4.15 676 242 245 3 846.28 3.08 677 243 246 3 894.22 0.60