BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 13 9 619.69 4.58 2 4 15 11 635.06 2.14 3 4 47 43 647.82 8.29 4 4 48 44 647.80 8.29 5 4 51 47 647.86 8.32 6 7 47 40 651.96 7.46 7 8 11 3 853.19 3.98 8 8 13 5 810.15 1.67 9 8 40 32 695.21 4.50 10 8 43 35 678.27 3.97 11 8 44 36 677.72 4.24 12 9 36 27 722.40 1.02 13 9 40 31 722.32 0.98 14 10 24 14 723.43 0.91 15 10 25 15 722.70 1.02 16 10 26 16 722.69 1.02 17 10 36 26 722.44 1.03 18 10 37 27 722.38 1.01 19 10 40 30 722.38 1.01 20 11 22 11 724.48 0.90 21 11 23 12 724.22 0.97 22 11 24 13 723.48 0.89 23 11 25 14 723.41 0.91 24 11 37 26 722.47 1.04 25 11 40 29 722.43 1.03 26 11 41 30 722.40 1.02 27 11 44 33 722.34 1.03 28 12 22 10 724.50 0.91 29 12 23 11 724.25 0.98 30 12 25 13 723.61 0.95 31 13 21 8 724.97 0.67 32 13 22 9 724.49 0.90 33 13 44 31 722.45 1.04 34 13 48 35 722.31 1.07 35 14 20 6 725.59 0.83 36 14 21 7 725.15 0.76 37 14 23 9 725.06 0.81 38 14 198 184 208.18 0.79 39 15 18 3 873.64 1.36 40 15 19 4 742.46 5.29 41 15 20 5 725.67 0.87 42 15 48 33 722.41 1.06 43 15 51 36 721.29 1.19 44 15 53 38 653.07 8.63 45 15 54 39 652.94 8.58 46 16 19 3 846.98 3.14 47 16 21 5 761.52 14.83 48 16 198 182 208.27 0.82 49 16 206 190 202.07 0.29 50 17 206 189 202.13 0.27 51 17 209 192 195.07 0.78 52 18 53 35 691.45 8.34 53 18 54 36 653.13 8.60 54 18 55 37 653.00 8.60 55 18 56 38 652.95 8.58 56 18 209 191 195.03 0.80 57 19 56 37 652.86 8.59 58 19 58 39 652.54 8.58 59 19 202 183 204.32 0.59 60 19 205 186 202.15 0.26 61 19 206 187 202.08 0.29 62 19 209 190 195.03 0.79 63 20 48 28 722.39 1.04 64 20 54 34 652.94 8.58 65 20 56 36 652.73 8.54 66 20 57 37 650.97 7.82 67 20 58 38 650.96 7.82 68 21 58 37 650.64 7.92 69 21 112 91 508.01 1.54 70 21 198 177 208.06 0.72 71 21 202 181 204.31 0.59 72 22 41 19 722.50 1.02 73 22 44 22 722.45 1.03 74 22 48 26 722.32 1.08 75 22 57 35 650.93 7.82 76 22 58 36 650.63 7.92 77 22 59 37 648.79 8.30 78 22 60 38 648.72 8.27 79 23 60 37 648.49 8.17 80 23 61 38 648.29 8.25 81 23 62 39 648.25 8.27 82 24 31 7 728.87 0.83 83 24 37 13 722.56 1.02 84 24 41 17 722.46 1.03 85 24 59 35 648.79 8.30 86 24 60 36 648.49 8.17 87 24 61 37 648.24 8.26 88 24 62 38 648.08 8.19 89 24 67 43 573.93 5.73 90 24 97 73 548.86 2.37 91 25 31 6 728.98 0.79 92 25 62 37 648.01 8.21 93 26 29 3 838.20 0.54 94 26 30 4 790.89 7.20 95 26 31 5 728.98 0.79 96 26 32 6 728.98 0.79 97 26 34 8 728.95 0.79 98 26 37 11 728.75 0.78 99 26 71 45 565.22 3.16 100 27 30 3 827.11 10.91 101 27 31 4 750.97 10.08 102 27 62 35 648.23 8.23 103 27 63 36 648.00 8.23 104 29 32 3 849.86 0.46 105 30 64 34 648.03 8.21 106 30 65 35 644.94 7.03 107 30 67 37 624.26 2.14 108 30 68 38 573.95 5.71 109 30 116 86 505.97 1.54 110 31 61 30 648.28 8.26 111 31 62 31 648.28 8.26 112 31 65 34 643.12 6.22 113 31 66 35 638.92 6.58 114 31 67 36 573.92 5.73 115 32 37 5 815.95 5.24 116 32 67 35 591.24 14.37 117 32 69 37 573.70 5.74 118 32 70 38 570.95 5.99 119 32 71 39 553.76 1.75 120 33 37 4 815.95 5.24 121 33 38 5 808.50 8.97 122 33 67 34 598.07 6.34 123 33 69 36 573.04 5.46 124 33 70 37 557.67 1.57 125 33 71 38 553.78 1.75 126 33 97 64 548.88 2.37 127 33 98 65 548.85 2.37 128 33 120 87 476.94 2.25 129 33 121 88 476.93 2.25 130 34 37 3 842.01 6.86 131 34 38 4 825.73 0.40 132 34 71 37 553.70 1.70 133 34 74 40 553.07 1.46 134 35 38 3 843.82 2.63 135 35 39 4 826.69 0.08 136 35 74 39 553.09 1.45 137 35 75 40 551.41 2.08 138 35 78 43 552.12 1.59 139 35 90 55 548.99 2.31 140 36 39 3 843.73 2.58 141 36 40 4 830.35 1.92 142 37 40 3 847.59 6.70 143 37 41 4 830.34 1.92 144 37 97 60 548.71 2.31 145 38 41 3 841.08 1.06 146 38 42 4 830.48 2.31 147 38 93 55 548.94 2.32 148 38 94 56 548.79 2.35 149 38 97 59 548.67 2.30 150 39 42 3 838.46 0.43 151 39 43 4 836.69 0.79 152 39 89 50 549.18 2.31 153 39 93 54 548.79 2.35 154 40 43 3 839.21 0.21 155 40 44 4 836.68 0.79 156 41 44 3 836.68 0.79 157 41 45 4 836.25 1.00 158 41 59 18 648.81 8.31 159 41 97 56 548.56 2.30 160 42 45 3 853.81 5.23 161 42 46 4 839.56 1.89 162 42 93 51 548.79 2.35 163 42 97 55 548.53 2.29 164 42 101 59 535.82 0.80 165 43 46 3 850.27 2.46 166 43 47 4 830.75 2.52 167 44 47 3 840.77 2.48 168 44 48 4 830.75 2.52 169 45 48 3 860.35 3.04 170 45 49 4 834.59 2.94 171 45 57 12 650.91 7.82 172 45 97 52 548.56 2.30 173 45 101 56 535.82 0.80 174 45 102 57 535.79 0.80 175 45 105 60 533.36 0.46 176 46 49 3 865.34 0.54 177 46 50 4 839.71 0.40 178 46 101 55 540.01 1.30 179 47 50 3 862.56 0.85 180 47 51 4 839.69 0.40 181 48 51 3 833.01 1.71 182 48 54 6 652.90 8.59 183 48 57 9 652.54 8.62 184 48 105 57 533.49 0.42 185 48 107 59 532.56 0.80 186 49 105 56 533.49 0.42 187 49 106 57 533.36 0.45 188 49 107 58 532.56 0.80 189 51 54 3 848.34 2.95 190 51 107 56 533.42 0.43 191 54 57 3 909.59 1.54 192 54 107 53 532.99 0.60 193 55 107 52 532.97 0.59 194 55 108 53 532.54 0.81 195 55 209 154 195.02 0.80 196 56 108 52 531.84 0.91 197 56 110 54 528.14 0.03 198 56 205 149 202.16 0.26 199 56 208 152 195.12 0.80 200 56 209 153 195.02 0.80 201 57 105 48 533.96 0.21 202 57 107 50 532.46 0.82 203 57 108 51 531.40 0.70 204 57 109 52 510.51 0.75 205 57 110 53 510.12 0.56 206 58 110 52 508.24 1.48 207 58 112 54 508.03 1.54 208 58 201 143 204.53 0.65 209 58 205 147 202.16 0.26 210 59 66 7 616.85 1.56 211 59 97 38 548.88 2.39 212 59 102 43 535.82 0.78 213 59 110 51 508.24 1.48 214 59 111 52 508.03 1.52 215 59 112 53 507.91 1.52 216 59 124 65 476.69 2.23 217 59 128 69 473.41 1.46 218 60 66 6 577.65 7.45 219 60 112 52 507.79 1.57 220 60 114 54 506.97 1.26 221 61 65 4 687.68 17.81 222 61 66 5 574.01 5.74 223 61 114 53 506.82 1.23 224 63 114 51 507.47 1.50 225 64 114 50 507.50 1.50 226 64 115 51 506.81 1.23 227 64 116 52 506.25 1.51 228 65 114 49 506.99 1.27 229 65 115 50 506.25 1.51 230 65 116 51 506.09 1.48 231 66 111 45 508.16 1.48 232 66 112 46 508.00 1.49 233 66 113 47 507.00 1.26 234 66 114 48 506.95 1.26 235 66 115 49 506.61 1.33 236 66 116 50 503.54 0.23 237 66 117 51 502.88 0.07 238 66 121 55 477.24 2.35 239 66 124 58 476.95 2.23 240 66 125 59 476.83 2.15 241 67 116 49 503.45 0.28 242 67 117 50 491.41 1.92 243 67 118 51 486.32 0.62 244 67 121 54 476.95 2.24 245 68 118 50 484.92 0.42 246 69 118 49 480.46 0.75 247 69 120 51 477.17 2.14 248 69 121 52 476.93 2.25 249 70 75 5 823.50 2.93 250 71 74 3 862.12 0.64 251 71 75 4 830.82 0.73 252 72 75 3 836.04 1.14 253 72 76 4 830.83 0.73 254 72 148 76 334.07 9.71 255 73 76 3 855.90 3.75 256 73 77 4 831.61 1.12 257 74 77 3 842.47 2.43 258 74 78 4 838.34 4.50 259 75 78 3 855.89 4.28 260 75 79 4 837.51 1.74 261 75 87 12 549.97 2.42 262 75 90 15 549.64 2.27 263 75 91 16 549.93 2.43 264 76 79 3 845.59 2.29 265 76 80 4 830.40 1.81 266 77 80 3 845.63 2.27 267 77 81 4 838.49 5.83 268 78 81 3 845.62 2.27 269 78 82 4 838.49 5.83 270 78 86 8 550.06 2.40 271 78 90 12 549.97 2.43 272 79 82 3 839.47 5.34 273 79 87 8 550.00 2.41 274 79 90 11 550.00 2.41 275 82 86 4 808.51 2.36 276 83 86 3 845.95 1.59 277 84 87 3 860.91 2.76 278 85 88 3 845.92 4.26 279 85 89 4 831.00 1.48 280 86 89 3 831.01 1.48 281 86 90 4 830.97 1.50 282 87 90 3 842.39 1.56 283 87 91 4 828.45 2.75 284 88 91 3 842.40 1.56 285 88 92 4 833.12 0.42 286 89 92 3 835.98 1.00 287 89 93 4 835.08 1.36 288 90 93 3 840.89 0.18 289 90 94 4 835.08 1.37 290 91 94 3 846.21 4.18 291 91 95 4 835.09 1.37 292 92 95 3 844.94 6.29 293 92 96 4 830.47 0.93 294 93 96 3 838.09 0.12 295 93 97 4 834.06 1.89 296 94 97 3 871.56 1.78 297 94 98 4 818.71 4.95 298 94 152 58 333.82 9.64 299 95 98 3 848.20 9.91 300 95 99 4 812.25 4.91 301 95 100 5 783.77 0.83 302 95 152 57 333.81 9.64 303 96 100 4 720.71 23.75 304 97 101 4 720.62 23.78 305 97 102 5 658.22 7.40 306 97 124 27 476.59 2.18 307 98 102 4 693.21 24.54 308 98 123 25 476.63 2.19 309 98 124 26 476.53 2.16 310 98 127 29 474.28 1.35 311 99 102 3 789.38 7.25 312 99 103 4 658.15 7.44 313 99 123 24 476.63 2.19 314 99 127 28 474.28 1.35 315 99 152 53 333.83 9.64 316 99 153 54 333.82 9.64 317 101 105 4 631.41 48.57 318 102 105 3 817.38 6.58 319 102 109 7 529.99 1.05 320 102 124 22 476.60 2.19 321 102 127 25 474.27 1.35 322 102 128 26 474.21 1.37 323 103 127 24 475.21 1.82 324 103 224 121 128.80 0.25 325 103 228 125 125.50 0.07 326 104 221 117 129.08 0.28 327 104 224 120 128.81 0.25 328 104 225 121 128.50 0.10 329 105 109 4 824.38 5.66 330 105 220 115 129.16 0.29 331 105 221 116 129.09 0.28 332 106 217 111 129.15 0.28 333 106 221 115 129.10 0.29 334 107 220 113 129.16 0.29 335 108 129 21 473.45 1.48 336 108 208 100 195.99 1.24 337 108 216 108 129.19 0.29 338 108 220 112 129.16 0.30 339 109 128 19 473.81 1.33 340 109 129 20 473.45 1.48 341 109 130 21 473.44 1.48 342 110 128 18 474.19 1.39 343 110 130 20 470.89 0.76 344 110 131 21 454.50 1.19 345 110 132 22 451.67 2.21 346 110 201 91 204.56 0.67 347 110 204 94 203.62 0.27 348 110 205 95 202.22 0.26 349 110 208 98 201.25 0.49 350 111 125 14 476.64 2.21 351 111 128 17 473.51 1.50 352 111 130 19 469.94 0.12 353 111 131 20 446.90 3.39 354 111 132 21 441.69 2.10 355 112 132 20 439.88 1.50 356 112 197 85 208.34 0.82 357 112 198 86 208.33 0.82 358 112 201 89 204.53 0.66 359 113 117 4 813.69 2.65 360 113 125 12 476.85 2.16 361 113 132 19 441.32 2.21 362 113 133 20 424.19 0.06 363 113 134 21 423.67 0.21 364 113 137 24 422.00 0.56 365 113 139 26 421.98 0.55 366 113 197 84 208.40 0.83 367 114 134 20 422.34 0.41 368 114 194 80 216.78 3.36 369 114 197 83 208.34 0.81 370 115 137 22 421.93 0.57 371 116 138 22 421.92 0.57 372 117 122 5 812.29 3.80 373 117 125 8 649.28 39.77 374 117 138 21 422.00 0.56 375 117 139 22 421.88 0.57 376 117 140 23 421.69 0.66 377 118 121 3 863.73 4.71 378 118 122 4 849.09 2.60 379 118 138 20 422.02 0.55 380 118 139 21 421.89 0.56 381 118 140 22 421.63 0.69 382 118 142 24 416.66 1.38 383 118 176 58 252.71 1.99 384 119 122 3 872.03 0.56 385 119 140 21 421.69 0.66 386 119 141 22 419.78 0.10 387 119 143 24 403.38 0.54 388 119 154 35 333.82 9.63 389 120 123 3 872.75 0.62 390 120 124 4 856.44 0.25 391 120 143 23 403.38 0.54 392 120 154 34 333.81 9.64 393 121 124 3 863.34 0.88 394 121 125 4 842.13 0.86 395 122 125 3 840.58 1.36 396 122 126 4 837.63 0.10 397 122 139 17 421.93 0.55 398 122 140 18 421.74 0.64 399 122 141 19 421.61 0.67 400 122 154 32 333.81 9.64 401 122 157 35 333.43 9.47 402 122 158 36 333.25 9.51 403 122 177 55 252.70 1.99 404 122 187 65 245.69 0.98 405 123 126 3 851.82 2.55 406 123 127 4 834.49 1.47 407 123 153 30 333.82 9.64 408 123 154 31 333.79 9.64 409 123 157 34 333.24 9.53 410 124 127 3 828.28 1.28 411 124 128 4 828.26 1.27 412 125 128 3 844.03 3.30 413 125 131 6 504.32 20.72 414 125 139 14 421.97 0.55 415 125 187 62 245.67 0.96 416 125 188 63 245.46 0.93 417 126 157 31 333.19 9.52 418 126 160 34 327.10 7.83 419 126 161 35 317.94 3.54 420 126 187 61 245.66 0.97 421 127 224 97 128.80 0.26 422 129 220 91 129.18 0.30 423 129 224 95 128.82 0.27 424 130 189 59 245.00 1.12 425 130 204 74 204.36 0.62 426 130 208 78 202.11 0.26 427 131 188 57 245.20 1.04 428 131 189 58 244.99 1.13 429 131 190 59 244.90 1.15 430 132 190 58 244.86 1.17 431 132 192 60 244.20 0.87 432 132 197 65 208.42 0.83 433 132 200 68 208.19 0.83 434 132 201 69 206.97 0.47 435 132 204 72 204.40 0.60 436 133 137 4 792.36 16.97 437 133 139 6 764.56 6.85 438 133 179 46 250.80 1.51 439 133 182 49 250.61 1.40 440 133 188 55 245.53 0.89 441 133 190 57 244.87 1.17 442 133 191 58 244.26 0.91 443 133 192 59 244.16 0.89 444 133 197 64 208.41 0.83 445 134 137 3 823.80 1.26 446 134 179 45 250.84 1.49 447 134 191 57 244.36 0.95 448 134 192 58 244.15 0.89 449 134 194 60 220.88 1.32 450 134 197 63 208.42 0.83 451 135 179 44 250.84 1.49 452 135 180 45 250.84 1.49 453 135 191 56 244.38 0.94 454 135 192 57 244.28 0.93 455 135 193 58 244.13 0.90 456 136 193 57 244.12 0.90 457 136 194 58 241.73 0.42 458 137 178 41 252.16 1.94 459 137 179 42 250.86 1.48 460 138 176 38 252.69 1.99 461 138 177 39 252.58 2.05 462 138 178 40 252.16 1.95 463 139 176 37 252.70 1.99 464 139 177 38 252.55 2.04 465 139 179 40 251.98 1.85 466 139 182 43 250.33 1.22 467 139 187 48 245.82 0.97 468 139 188 49 245.79 0.97 469 140 176 36 252.70 1.99 470 141 144 3 903.97 0.43 471 141 154 13 333.85 9.63 472 141 158 17 333.78 9.61 473 141 171 30 256.12 1.18 474 141 174 33 254.30 1.31 475 142 145 3 879.47 1.37 476 142 146 4 855.19 2.11 477 142 174 32 254.34 1.32 478 143 146 3 852.23 2.91 479 143 147 4 835.73 1.58 480 143 148 5 706.16 30.70 481 143 149 6 334.35 9.78 482 143 150 7 334.13 9.69 483 143 154 11 334.04 9.65 484 144 147 3 846.62 5.71 485 144 148 4 804.44 0.74 486 144 171 27 256.22 1.22 487 144 172 28 256.20 1.21 488 144 173 29 256.15 1.20 489 144 174 30 255.44 1.26 490 145 174 29 255.67 1.23 491 150 154 4 759.09 1.40 492 150 155 5 710.25 25.82 493 150 171 21 256.21 1.17 494 151 154 3 862.50 2.83 495 153 156 3 834.33 0.01 496 153 157 4 809.05 7.88 497 154 157 3 834.09 2.00 498 154 158 4 815.80 5.26 499 155 158 3 834.09 2.00 500 155 159 4 829.96 2.56 501 155 171 16 256.39 1.15 502 156 159 3 833.75 0.68 503 156 160 4 829.96 2.56 504 157 160 3 843.14 3.99 505 157 161 4 830.00 2.58 506 158 161 3 835.74 1.67 507 158 162 4 778.72 9.18 508 158 167 9 256.43 1.15 509 158 170 12 256.42 1.14 510 158 171 13 256.43 1.14 511 158 187 29 245.81 0.96 512 160 227 67 126.30 0.97 513 161 186 25 245.75 0.94 514 161 187 26 245.77 0.95 515 162 166 4 819.88 2.48 516 162 167 5 774.84 1.48 517 162 187 25 245.84 0.97 518 163 166 3 845.12 4.71 519 163 167 4 816.91 4.03 520 164 167 3 852.20 1.16 521 164 168 4 836.90 0.61 522 165 168 3 849.79 2.37 523 165 169 4 838.97 0.42 524 166 169 3 853.68 6.93 525 166 170 4 838.98 0.41 526 166 185 19 249.03 1.38 527 167 170 3 853.87 6.83 528 167 171 4 838.98 0.41 529 168 171 3 840.18 0.18 530 168 172 4 838.97 0.42 531 169 172 3 849.19 1.33 532 169 173 4 818.00 6.95 533 169 175 6 763.30 5.03 534 170 173 3 850.41 0.72 535 170 174 4 785.85 6.94 536 170 175 5 741.18 7.65 537 170 177 7 543.63 106.39 538 170 181 11 258.82 1.10 539 177 181 4 806.08 0.14 540 177 182 5 802.36 1.73 541 177 187 10 247.19 0.49 542 178 181 3 845.57 5.18 543 178 182 4 819.09 5.45 544 179 182 3 824.40 2.79 545 179 183 4 824.31 2.85 546 179 191 12 244.47 0.98 547 180 183 3 868.36 5.52 548 180 184 4 819.20 5.41 549 180 191 11 244.88 1.17 550 181 184 3 847.08 5.35 551 181 185 4 822.24 7.06 552 182 185 3 851.03 3.38 553 182 186 4 831.34 0.85 554 182 187 5 673.00 62.36 555 182 188 6 290.00 23.05 556 183 186 3 831.35 0.85 557 183 188 5 258.07 5.16 558 183 190 7 245.62 0.97 559 183 191 8 245.50 0.92 560 190 200 10 208.51 0.85 561 192 196 4 784.75 3.62 562 192 197 5 618.79 11.23 563 192 200 8 241.24 15.46 564 193 196 3 846.60 5.89 565 193 197 4 800.22 4.12 566 194 197 3 844.58 6.90 567 196 199 3 836.82 0.18 568 196 200 4 812.96 2.23 569 197 200 3 846.05 3.21 570 197 201 4 825.31 3.62 571 198 201 3 836.36 6.46 572 198 202 4 825.34 3.64 573 199 202 3 831.64 6.04 574 199 203 4 827.25 4.59 575 200 203 3 840.31 1.62 576 200 204 4 826.85 4.79 577 201 204 3 837.12 0.03 578 201 205 4 826.40 4.69 579 202 205 3 836.50 0.35 580 202 206 4 824.85 3.94 581 203 206 3 854.11 1.55 582 203 207 4 833.62 0.46 583 204 207 3 842.27 0.06 584 204 208 4 838.35 1.91 585 205 208 3 838.35 1.92 586 205 209 4 830.74 5.72 587 206 209 3 833.97 4.11 588 206 210 4 833.98 4.12 589 207 210 3 853.91 6.86 590 207 211 4 844.92 3.17 591 208 211 3 846.67 2.29 592 208 216 8 289.88 15.20 593 211 216 5 805.57 2.25 594 212 215 3 857.30 2.44 595 212 216 4 823.90 6.42 596 212 249 37 25.18 0.16 597 213 216 3 843.17 3.20 598 213 217 4 824.76 6.00 599 214 217 3 839.71 3.94 600 214 218 4 826.39 5.19 601 214 245 31 28.68 0.70 602 214 248 34 26.07 0.58 603 214 249 35 25.32 0.20 604 215 218 3 837.61 4.99 605 215 219 4 826.40 5.19 606 215 245 30 28.68 0.70 607 216 219 3 848.36 2.35 608 216 220 4 826.52 5.09 609 217 220 3 848.96 2.06 610 217 221 4 826.51 5.09 611 218 221 3 850.01 3.17 612 218 222 4 826.51 5.08 613 218 241 23 31.34 1.48 614 218 245 27 28.68 0.70 615 219 222 3 832.73 1.94 616 219 223 4 826.12 4.88 617 220 223 3 844.00 0.73 618 220 224 4 827.07 4.74 619 221 224 3 844.12 0.80 620 221 225 4 839.53 1.50 621 221 237 16 45.80 1.27 622 221 241 20 33.54 0.85 623 222 225 3 839.66 1.43 624 222 226 4 827.08 4.73 625 222 237 15 45.74 1.24 626 222 238 16 43.43 0.61 627 222 241 19 35.36 0.94 628 223 226 3 843.92 5.86 629 223 227 4 838.81 1.14 630 224 227 3 857.25 0.82 631 225 228 3 851.05 2.29 632 225 229 4 838.64 1.23 633 225 237 12 46.38 1.02 634 226 229 3 838.66 1.22 635 226 234 8 75.46 0.28 636 226 238 12 46.37 1.02 637 229 233 4 827.09 2.44 638 229 234 5 807.14 2.85 639 229 237 8 316.78 9.64 640 230 233 3 836.76 7.28 641 230 234 4 827.76 2.79 642 231 234 3 849.68 0.82 643 231 235 4 836.75 7.28 644 232 235 3 849.68 0.82 645 232 236 4 846.19 2.57 646 233 236 3 852.39 0.53 647 233 237 4 825.98 3.67 648 234 237 3 829.43 3.03 649 234 238 4 814.63 0.67 650 235 239 4 814.63 0.67 651 236 239 3 854.46 1.12 652 236 240 4 815.00 0.48 653 237 240 3 843.91 3.97 654 237 241 4 826.60 1.60 655 238 241 3 835.69 2.94 656 238 242 4 826.61 1.60 657 239 242 3 828.85 0.48 658 239 243 4 826.60 1.60 659 240 243 3 825.61 1.10 660 240 244 4 820.75 3.57 661 241 244 3 827.96 0.01 662 241 245 4 820.79 3.58 663 242 245 3 839.60 3.33 664 242 246 4 821.88 3.05 665 243 246 3 823.78 4.00 666 243 247 4 821.88 3.04 667 244 247 3 830.62 0.58 668 244 248 4 821.87 3.04 669 245 248 3 829.46 4.20 670 245 249 4 820.63 3.65 671 246 249 3 829.74 0.99