BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 6 9 3 841.58 1.50 2 6 19 13 369.31 152.62 3 6 56 50 350.07 153.09 4 7 10 3 844.44 2.93 5 7 11 4 815.52 2.85 6 8 11 3 826.30 2.54 7 8 12 4 824.75 3.32 8 8 17 9 369.56 152.50 9 8 52 44 350.00 153.20 10 8 53 45 359.88 149.41 11 8 56 48 361.38 148.66 12 8 59 51 361.04 148.22 13 9 17 8 369.47 152.46 14 9 19 10 369.74 152.53 15 9 25 16 369.65 152.49 16 11 52 41 364.30 148.77 17 12 15 3 826.83 0.43 18 12 17 5 802.58 0.04 19 12 45 33 363.47 149.34 20 12 48 36 364.44 148.84 21 12 49 37 364.41 148.81 22 12 52 40 364.54 148.71 23 13 30 17 371.64 151.48 24 13 41 28 365.04 148.96 25 14 29 15 371.64 151.49 26 14 30 16 371.64 151.48 27 14 31 17 371.62 151.47 28 14 36 22 366.07 148.70 29 14 42 28 364.99 148.93 30 14 46 32 365.56 148.62 31 15 27 12 371.84 151.50 32 15 28 13 371.72 151.50 33 15 29 14 371.68 151.50 34 15 30 15 371.67 151.48 35 15 46 31 365.75 148.55 36 15 49 34 365.74 148.53 37 16 26 10 372.42 151.38 38 16 27 11 371.85 151.50 39 16 28 12 371.79 151.47 40 16 30 14 371.75 151.47 41 17 25 8 372.45 151.38 42 17 26 9 372.41 151.37 43 17 27 10 371.93 151.47 44 17 53 36 365.88 148.44 45 18 25 7 372.48 151.37 46 18 26 8 372.43 151.37 47 18 203 185 175.06 11.43 48 19 23 4 660.75 61.38 49 19 24 5 373.19 151.21 50 19 25 6 372.91 151.15 51 19 56 37 407.75 127.48 52 19 58 39 408.14 127.06 53 19 59 40 366.49 147.85 54 20 23 3 830.99 3.73 55 20 24 4 413.41 131.12 56 20 203 183 176.22 10.86 57 22 60 38 408.29 126.98 58 22 210 188 168.31 7.02 59 23 58 35 409.37 126.45 60 23 59 36 408.81 126.70 61 23 60 37 408.19 126.92 62 23 61 38 408.82 126.61 63 24 61 37 409.13 126.28 64 24 63 39 411.45 124.61 65 24 203 179 181.37 8.26 66 24 206 182 172.06 7.73 67 24 207 183 172.06 7.73 68 24 210 186 166.99 5.20 69 25 53 28 414.10 124.34 70 25 59 34 409.31 126.34 71 25 61 36 409.16 126.27 72 25 62 37 408.22 125.80 73 25 63 38 411.81 124.00 74 26 63 37 411.86 123.97 75 26 65 39 415.67 122.03 76 26 199 173 186.43 7.40 77 26 203 177 184.60 6.64 78 27 53 26 416.97 122.83 79 27 62 35 415.57 122.12 80 27 63 36 415.54 122.11 81 27 64 37 415.41 122.03 82 27 65 38 416.37 121.55 83 28 65 37 416.30 121.52 84 28 66 38 416.35 121.40 85 28 67 39 416.36 121.39 86 29 36 7 419.03 122.36 87 29 46 17 418.34 122.37 88 29 64 35 416.51 121.48 89 29 65 36 416.41 121.42 90 29 66 37 416.35 121.39 91 29 67 38 416.20 121.30 92 29 71 42 353.09 89.75 93 29 72 43 353.11 89.75 94 30 33 3 815.41 1.51 95 30 34 4 721.32 2.33 96 30 36 6 419.02 122.36 97 30 67 37 416.56 121.12 98 31 34 3 799.32 0.75 99 31 35 4 754.09 3.65 100 31 36 5 425.20 125.25 101 31 37 6 425.20 125.25 102 33 67 34 417.98 120.60 103 34 67 33 433.78 112.69 104 35 67 32 433.61 112.60 105 35 68 33 435.26 111.77 106 35 69 34 435.25 111.77 107 35 70 35 432.12 111.86 108 36 42 6 796.64 3.20 109 36 46 10 773.75 6.52 110 36 72 36 371.94 80.33 111 36 74 38 408.12 62.08 112 37 74 37 408.12 62.08 113 37 75 38 408.16 61.91 114 37 76 39 408.20 61.88 115 38 42 4 808.32 6.28 116 38 43 5 799.47 1.87 117 38 46 8 782.30 3.54 118 38 72 34 408.49 62.08 119 38 74 36 408.44 62.01 120 38 75 37 408.19 61.88 121 38 76 38 408.23 61.87 122 38 80 42 415.63 57.85 123 38 120 82 383.69 49.66 124 38 121 83 380.34 47.97 125 38 124 86 376.33 45.98 126 39 42 3 821.31 1.94 127 39 43 4 818.53 1.18 128 39 76 37 408.25 61.84 129 39 79 40 415.62 57.86 130 39 80 41 415.61 57.84 131 40 43 3 823.79 0.70 132 40 44 4 818.54 1.16 133 40 79 39 414.21 57.14 134 40 80 40 413.44 56.75 135 41 44 3 826.53 0.67 136 41 45 4 814.48 0.88 137 42 45 3 827.22 1.32 138 42 46 4 809.52 3.34 139 42 47 5 815.44 1.36 140 43 47 4 826.70 0.49 141 43 80 37 433.44 47.24 142 43 92 49 435.88 43.86 143 43 95 52 435.86 43.86 144 43 96 53 435.82 43.86 145 44 47 3 866.08 1.81 146 44 95 51 435.88 43.84 147 45 48 3 846.39 5.05 148 45 49 4 811.50 4.31 149 46 49 3 822.92 1.39 150 46 50 4 811.50 4.32 151 46 99 53 435.88 43.79 152 46 124 78 408.10 29.95 153 47 50 3 825.06 0.59 154 47 51 4 810.47 4.85 155 47 95 48 435.99 43.83 156 47 98 51 435.94 43.80 157 47 99 52 435.88 43.80 158 47 102 55 435.66 43.59 159 48 51 3 823.91 0.45 160 48 52 4 810.47 4.86 161 49 52 3 822.52 1.15 162 49 53 4 809.83 5.17 163 50 53 3 812.77 3.71 164 50 54 4 813.25 3.41 165 50 64 14 503.88 77.80 166 50 99 49 436.17 43.65 167 50 102 52 435.85 43.46 168 50 103 53 435.53 43.48 169 50 106 56 435.29 43.16 170 50 124 74 408.36 29.79 171 51 54 3 820.07 0.01 172 51 55 4 808.94 1.26 173 51 102 51 435.81 43.48 174 52 55 3 825.75 2.85 175 52 56 4 812.65 3.11 176 53 56 3 838.37 5.58 177 53 59 6 695.64 8.17 178 53 62 9 504.37 77.73 179 53 106 53 435.32 43.15 180 54 105 51 435.38 43.16 181 54 106 52 435.29 43.16 182 56 59 3 844.86 5.88 183 57 215 158 195.65 17.54 184 57 218 161 195.61 17.53 185 59 62 3 868.37 1.26 186 59 108 49 616.03 47.24 187 60 108 48 615.58 47.48 188 60 109 49 615.58 47.48 189 60 210 150 227.04 22.29 190 60 212 152 224.43 23.58 191 60 213 153 221.78 24.07 192 60 214 154 195.64 17.54 193 60 217 157 195.61 17.54 194 61 108 47 615.40 47.56 195 61 109 48 615.15 47.69 196 61 111 50 612.89 48.81 197 61 206 145 236.93 24.69 198 61 209 148 227.56 22.33 199 61 210 149 224.56 23.59 200 61 217 156 195.59 17.54 201 62 106 44 616.25 47.33 202 62 108 46 615.51 47.63 203 62 109 47 614.72 48.00 204 62 110 48 605.69 52.41 205 62 111 49 605.51 52.50 206 63 111 48 605.36 52.58 207 63 113 50 605.22 52.63 208 63 199 136 259.99 28.98 209 63 202 139 252.12 27.09 210 63 203 140 252.05 27.07 211 63 206 143 236.95 24.70 212 64 103 39 616.77 47.13 213 64 110 46 605.87 52.49 214 64 111 47 605.55 52.58 215 64 112 48 605.23 52.65 216 64 113 49 605.13 52.69 217 64 124 60 543.65 37.80 218 65 71 6 600.95 34.23 219 65 113 48 605.13 52.70 220 65 115 50 604.90 52.73 221 65 199 134 259.97 28.99 222 66 71 5 601.15 34.33 223 66 115 49 604.93 52.79 224 67 70 3 862.09 1.98 225 67 115 48 605.02 52.84 226 68 115 47 605.15 52.85 227 69 115 46 605.15 52.85 228 69 116 47 605.00 52.85 229 69 136 67 514.11 52.34 230 69 195 126 295.74 46.73 231 70 73 3 841.78 4.16 232 70 115 45 605.17 52.88 233 70 116 46 604.94 52.88 234 70 117 47 604.80 52.95 235 71 114 43 605.21 52.88 236 71 115 44 605.14 52.90 237 71 116 45 605.04 52.93 238 71 117 46 604.78 53.03 239 71 118 47 604.19 53.26 240 71 120 49 596.91 56.87 241 71 121 50 594.74 57.85 242 71 122 51 566.34 48.83 243 71 124 53 554.01 42.84 244 71 125 54 552.58 42.17 245 72 117 45 604.83 53.05 246 72 118 46 596.95 56.88 247 72 119 47 596.95 56.88 248 72 120 48 596.85 56.89 249 72 121 49 594.48 57.98 250 72 124 52 562.31 46.95 251 73 117 44 604.62 53.16 252 73 119 46 596.95 56.88 253 73 120 47 596.95 56.88 254 74 120 46 597.04 56.94 255 75 80 5 824.80 0.66 256 75 120 45 597.05 56.94 257 76 79 3 831.12 0.94 258 76 80 4 824.80 0.65 259 77 80 3 831.12 0.94 260 77 83 6 794.82 1.91 261 77 147 70 407.25 49.50 262 78 81 3 857.13 1.83 263 80 83 3 884.25 1.03 264 82 85 3 831.41 1.91 265 82 88 6 617.75 47.02 266 82 251 169 68.11 15.91 267 83 89 6 617.76 47.02 268 83 247 164 73.92 15.00 269 84 247 163 74.80 14.55 270 84 248 164 73.80 14.98 271 84 250 166 69.78 16.44 272 84 251 167 68.11 15.91 273 85 88 3 807.86 0.61 274 85 89 4 799.40 0.89 275 85 251 166 72.36 15.16 276 86 89 3 814.92 2.37 277 86 90 4 803.20 2.78 278 86 248 162 75.12 14.39 279 87 90 3 825.82 1.35 280 88 91 3 834.66 3.06 281 88 92 4 825.45 1.31 282 89 92 3 833.14 4.33 283 89 93 4 810.34 4.15 284 89 240 151 96.33 15.27 285 89 243 154 92.39 13.88 286 89 244 155 84.45 12.94 287 89 247 158 75.14 14.39 288 90 93 3 809.94 0.27 289 90 94 4 805.39 1.68 290 91 94 3 823.22 0.64 291 91 95 4 805.36 1.68 292 92 95 3 823.22 0.64 293 92 96 4 802.36 4.27 294 93 96 3 830.41 1.31 295 93 97 4 813.16 4.86 296 93 237 144 99.86 13.88 297 93 240 147 93.02 13.61 298 93 241 148 93.02 13.61 299 94 97 3 833.22 0.29 300 94 98 4 822.49 0.21 301 95 98 3 825.73 1.31 302 95 99 4 823.30 0.61 303 96 99 3 838.55 1.40 304 96 100 4 830.14 2.81 305 96 236 140 100.55 14.19 306 96 240 144 93.03 13.60 307 97 100 3 841.98 2.12 308 97 101 4 826.50 0.98 309 97 236 139 100.52 14.18 310 97 237 140 99.82 13.87 311 97 240 143 93.03 13.60 312 98 101 3 827.78 1.63 313 98 102 4 826.51 0.99 314 99 102 3 826.70 1.07 315 99 103 4 826.51 0.98 316 99 124 25 543.75 37.88 317 100 103 3 830.35 2.90 318 100 104 4 820.35 4.06 319 100 123 23 543.78 37.87 320 100 124 24 543.75 37.88 321 100 127 27 543.20 38.11 322 100 153 53 400.32 47.65 323 100 154 54 400.26 47.66 324 100 232 132 108.20 14.85 325 100 236 136 99.89 13.84 326 101 104 3 831.89 3.67 327 101 105 4 812.11 0.06 328 102 105 3 830.78 3.39 329 102 106 4 824.19 5.98 330 103 106 3 831.61 3.81 331 103 124 21 543.72 37.89 332 103 127 24 541.12 39.05 333 103 221 118 195.44 17.55 334 104 127 23 541.12 39.04 335 104 221 117 195.44 17.55 336 104 228 124 109.02 14.88 337 104 229 125 108.93 14.89 338 104 232 128 108.50 14.69 339 105 229 124 108.93 14.89 340 106 110 4 821.01 8.11 341 106 221 115 195.44 17.55 342 107 218 111 195.52 17.54 343 107 221 114 195.44 17.55 344 107 222 115 195.41 17.55 345 108 218 110 195.52 17.55 346 108 221 113 195.44 17.55 347 109 129 20 539.27 39.86 348 109 209 100 228.81 21.71 349 109 217 108 195.58 17.53 350 109 221 112 195.41 17.56 351 110 128 18 539.29 39.87 352 110 129 19 538.15 40.43 353 110 130 20 538.15 40.43 354 110 221 111 195.43 17.55 355 111 130 19 537.98 40.51 356 111 132 21 534.14 42.42 357 111 202 91 252.21 27.07 358 111 205 94 246.93 24.60 359 111 206 95 240.30 23.48 360 111 209 98 228.88 21.68 361 112 125 13 543.53 37.80 362 112 128 16 538.91 40.11 363 112 130 18 538.07 40.51 364 112 131 19 530.62 44.16 365 112 132 20 530.61 44.16 366 113 132 19 530.46 44.25 367 113 198 85 260.06 28.97 368 113 199 86 260.02 28.98 369 113 202 89 252.53 27.27 370 114 118 4 808.76 7.40 371 114 125 11 543.85 37.80 372 114 131 17 537.44 40.83 373 114 132 18 530.52 44.24 374 114 133 19 524.12 47.43 375 114 134 20 524.03 47.43 376 114 137 23 474.46 48.67 377 114 139 25 449.67 60.69 378 114 198 84 260.06 28.97 379 115 134 19 517.48 50.71 380 115 198 83 260.14 28.94 381 116 134 18 517.58 50.70 382 116 136 20 514.09 52.33 383 116 137 21 451.66 61.59 384 117 137 20 486.57 54.81 385 117 138 21 450.15 60.83 386 118 122 4 602.41 63.03 387 118 125 7 727.04 22.82 388 118 138 20 450.18 60.83 389 118 139 21 445.11 58.69 390 118 140 22 445.11 58.69 391 119 138 19 462.43 66.89 392 119 140 21 444.86 58.81 393 119 141 22 439.17 61.30 394 119 142 23 439.07 61.27 395 119 143 24 439.07 61.27 396 120 142 22 439.07 61.27 397 120 143 23 434.84 62.48 398 121 124 3 838.01 3.28 399 121 153 32 400.45 47.64 400 122 125 3 836.15 4.21 401 122 126 4 817.94 1.79 402 122 159 37 400.28 47.66 403 123 126 3 818.23 1.92 404 123 127 4 827.45 2.69 405 123 153 30 400.43 47.62 406 123 154 31 400.29 47.66 407 123 155 32 400.27 47.66 408 123 158 35 400.11 47.61 409 124 127 3 843.16 0.42 410 124 128 4 827.45 2.69 411 125 128 3 837.68 2.81 412 125 131 6 771.01 2.51 413 125 139 14 445.15 58.66 414 126 131 5 809.65 3.92 415 126 158 32 400.10 47.60 416 126 159 33 400.05 47.58 417 126 162 36 398.95 47.87 418 126 188 62 314.03 42.90 419 127 158 31 400.05 47.58 420 127 221 94 195.43 17.55 421 127 228 101 109.07 14.86 422 128 131 3 881.06 1.49 423 128 221 93 195.42 17.55 424 129 221 92 195.44 17.55 425 130 190 60 313.64 42.94 426 130 205 75 251.50 26.82 427 130 209 79 233.44 23.04 428 131 188 57 314.04 42.91 429 131 189 58 313.75 42.98 430 131 190 59 313.62 42.96 431 131 191 60 313.60 42.97 432 132 191 59 313.47 43.02 433 132 193 61 312.75 43.34 434 132 198 66 260.14 28.93 435 132 201 69 259.52 29.11 436 132 202 70 254.02 26.62 437 132 205 73 253.93 26.59 438 133 137 4 770.87 14.55 439 133 180 47 319.05 40.98 440 133 189 56 313.83 43.02 441 133 191 58 313.69 43.02 442 133 192 59 312.87 43.30 443 133 193 60 312.78 43.34 444 133 198 65 260.14 28.94 445 134 137 3 828.38 2.06 446 134 180 46 319.10 40.99 447 134 193 59 312.64 43.38 448 134 195 61 298.37 47.93 449 134 198 64 260.17 28.95 450 135 180 45 319.35 40.87 451 135 192 57 313.54 43.11 452 135 193 58 312.90 43.32 453 135 194 59 312.72 43.38 454 136 195 59 309.33 45.03 455 137 179 42 319.39 40.88 456 137 180 43 319.10 41.00 457 138 177 39 340.56 38.55 458 138 178 40 332.43 34.51 459 138 179 41 319.41 40.89 460 139 159 20 400.32 47.65 461 139 171 32 398.48 48.04 462 139 177 38 343.19 39.87 463 139 178 39 332.41 34.50 464 139 183 44 319.24 40.82 465 139 189 50 314.15 42.94 466 140 171 31 398.44 48.06 467 140 175 35 371.93 41.12 468 140 176 36 371.50 40.93 469 140 177 37 343.19 39.87 470 141 144 3 880.57 1.59 471 141 151 10 401.10 47.58 472 141 155 14 400.48 47.63 473 141 171 30 398.48 48.04 474 141 172 31 398.36 48.09 475 141 175 34 371.95 41.11 476 142 146 4 812.64 2.25 477 142 175 33 375.41 42.85 478 143 146 3 835.91 3.63 479 143 147 4 795.40 5.95 480 144 147 3 809.30 0.58 481 144 148 4 795.39 5.95 482 144 149 5 637.48 54.88 483 144 150 6 612.54 57.68 484 144 151 7 401.88 47.34 485 144 153 9 401.88 47.34 486 144 155 11 401.27 47.65 487 144 243 99 91.45 12.83 488 145 148 3 802.52 1.16 489 145 149 4 777.83 3.41 490 145 151 6 613.77 57.08 491 145 172 27 398.50 48.05 492 145 173 28 398.45 48.06 493 145 175 30 397.69 48.31 494 146 175 29 397.44 48.55 495 147 250 103 70.73 14.60 496 148 246 98 84.70 12.73 497 149 243 94 92.70 13.44 498 149 246 97 80.99 14.09 499 149 247 98 80.98 14.10 500 149 250 101 72.47 14.87 501 150 242 92 93.05 13.58 502 150 243 93 91.06 12.80 503 150 246 96 84.38 12.41 504 151 155 4 741.68 3.17 505 151 156 5 651.57 59.09 506 152 156 4 771.49 6.89 507 152 236 84 105.12 14.84 508 152 239 87 93.14 13.59 509 152 240 88 93.11 13.58 510 152 243 91 88.89 11.92 511 153 156 3 885.87 1.00 512 153 236 83 103.34 15.18 513 153 240 87 93.06 13.59 514 153 243 90 86.86 12.94 515 154 157 3 825.30 1.69 516 154 158 4 807.42 0.19 517 154 236 82 102.34 14.70 518 155 158 3 825.31 1.69 519 155 159 4 804.83 1.49 520 155 160 5 804.83 1.49 521 155 172 17 398.31 48.01 522 156 160 4 819.87 1.03 523 156 172 16 398.47 48.03 524 156 235 79 106.73 15.57 525 156 236 80 105.31 14.88 526 156 239 83 97.29 12.79 527 157 160 3 849.44 2.92 528 157 161 4 812.76 2.52 529 157 231 74 109.01 14.86 530 157 232 75 108.98 14.85 531 157 235 78 105.33 14.87 532 157 236 79 105.30 14.86 533 158 161 3 809.50 1.25 534 158 162 4 801.77 2.97 535 158 228 70 109.21 14.84 536 158 232 74 108.96 14.86 537 159 162 3 804.86 1.43 538 159 163 4 801.77 2.97 539 159 168 9 398.48 48.04 540 159 171 12 398.49 48.03 541 159 172 13 398.47 48.03 542 159 178 19 363.25 44.69 543 159 188 29 314.16 42.90 544 160 164 4 538.70 3.19 545 160 165 5 398.84 47.87 546 160 168 8 398.50 48.02 547 160 172 12 398.49 48.02 548 162 188 26 314.10 42.89 549 163 167 4 677.17 24.55 550 163 168 5 399.59 48.57 551 163 171 8 611.44 57.41 552 163 178 15 366.50 43.13 553 163 186 23 315.88 42.10 554 163 188 25 314.33 42.86 555 164 168 4 727.03 1.04 556 165 169 4 795.49 4.29 557 166 169 3 829.00 5.18 558 166 170 4 815.87 1.06 559 167 170 3 824.45 5.53 560 167 171 4 805.84 3.94 561 167 176 9 399.19 48.62 562 167 178 11 398.21 49.07 563 167 186 19 317.79 41.20 564 168 171 3 816.28 1.27 565 168 172 4 805.85 3.94 566 169 172 3 829.10 3.19 567 169 173 4 799.71 7.00 568 170 173 3 823.67 0.47 569 170 174 4 789.25 4.57 570 170 176 6 611.78 57.68 571 171 175 4 779.20 7.78 572 171 176 5 611.77 57.68 573 171 178 7 611.76 57.68 574 172 175 3 802.50 1.33 575 178 182 4 792.37 0.86 576 178 183 5 792.26 0.84 577 178 186 8 577.81 74.52 578 179 182 3 802.57 5.90 579 179 183 4 798.57 1.02 580 180 183 3 809.00 2.69 581 180 184 4 802.20 5.72 582 180 192 12 313.64 43.09 583 181 184 3 827.68 3.73 584 181 185 4 802.20 5.72 585 181 192 11 313.89 43.06 586 182 185 3 823.73 3.00 587 182 186 4 807.53 3.05 588 183 186 3 821.46 4.12 589 183 187 4 807.53 3.05 590 183 188 5 737.29 24.41 591 183 189 6 472.93 36.37 592 184 187 3 823.72 3.00 593 184 189 5 362.54 18.84 594 184 190 6 362.53 18.84 595 184 191 7 362.53 18.83 596 184 192 8 314.16 42.99 597 187 190 3 866.94 1.52 598 189 192 3 899.56 3.08 599 191 201 10 260.20 28.89 600 191 205 14 254.17 26.54 601 193 197 4 802.69 2.79 602 193 198 5 527.67 104.81 603 193 201 8 260.23 28.91 604 194 197 3 830.89 2.52 605 194 198 4 780.31 13.98 606 195 198 3 830.89 2.52 607 197 200 3 818.20 1.47 608 197 201 4 816.88 0.82 609 198 201 3 826.63 5.69 610 198 202 4 805.62 0.33 611 199 202 3 822.40 3.58 612 199 203 4 805.62 0.33 613 200 203 3 820.08 4.74 614 200 204 4 804.71 0.78 615 201 204 3 807.90 0.17 616 201 205 4 808.27 2.58 617 202 205 3 825.02 4.86 618 202 206 4 814.38 0.46 619 203 206 3 814.38 0.46 620 203 207 4 814.38 0.46 621 204 207 3 819.38 2.17 622 204 208 4 814.38 0.47 623 205 208 3 821.06 1.29 624 205 209 4 813.25 1.03 625 206 209 3 818.99 2.34 626 206 210 4 806.14 2.52 627 207 210 3 826.61 3.59 628 207 211 4 812.70 5.80 629 208 211 3 835.71 0.33 630 209 212 3 838.47 3.80 631 209 217 8 624.92 35.81 632 210 217 7 649.23 47.65 633 212 216 4 802.75 2.57 634 212 217 5 784.61 4.72 635 213 216 3 825.85 1.28 636 213 217 4 804.86 3.62 637 214 217 3 829.82 0.71 638 214 218 4 805.85 3.13 639 215 218 3 822.60 2.92 640 215 219 4 803.74 2.07 641 216 219 3 818.01 0.62 642 216 220 4 795.35 2.12 643 217 220 3 825.53 8.55 644 217 221 4 810.32 1.81 645 218 221 3 822.11 7.71 646 218 222 4 810.32 1.81 647 219 222 3 810.31 1.81 648 219 223 4 810.31 1.81 649 220 223 3 811.79 1.07 650 220 224 4 809.10 0.27 651 221 224 3 823.89 4.99 652 221 225 4 809.11 0.27 653 222 225 3 822.48 2.94 654 222 226 4 794.07 7.78 655 225 228 3 824.77 5.12 656 225 229 4 784.91 2.77 657 226 229 3 827.15 3.93 658 226 230 4 801.93 3.29 659 227 230 3 832.41 1.45 660 227 231 4 797.84 2.57 661 228 231 3 829.06 0.96 662 228 232 4 815.06 6.04 663 229 232 3 845.05 7.04 664 229 233 4 814.24 6.46 665 230 233 3 817.30 4.92 666 230 234 4 807.56 3.10 667 231 234 3 833.35 12.88 668 231 235 4 812.25 4.32 669 232 235 3 829.78 8.27 670 232 236 4 796.90 2.14 671 233 236 3 828.94 2.85 672 235 238 3 839.76 0.16 673 235 239 4 810.50 0.90 674 236 239 3 813.07 2.18 675 236 240 4 810.50 0.91 676 237 240 3 829.14 0.54 677 237 241 4 804.40 3.98 678 238 241 3 808.46 1.98 679 238 242 4 805.01 4.29 680 239 242 3 817.83 5.00 681 239 243 4 804.55 4.04 682 240 243 3 809.04 2.28 683 240 244 4 805.25 4.39 684 241 244 3 814.25 3.19 685 241 245 4 810.97 1.54 686 242 245 3 814.27 3.19 687 242 246 4 810.99 1.54 688 243 246 3 819.22 5.66 689 243 247 4 811.01 1.55 690 244 247 3 811.06 1.57 691 244 248 4 811.02 1.56 692 245 248 3 825.25 5.16 693 245 249 4 808.72 2.71 694 246 249 3 817.99 1.53 695 246 250 4 808.71 2.71 696 247 250 3 836.45 3.06 697 248 251 3 852.65 2.40 698 249 252 3 831.88 7.99