BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 225 222 119.44 1.40 2 3 226 223 119.02 1.60 3 3 228 225 119.53 1.29 4 3 229 226 117.73 2.19 5 4 204 200 176.98 2.29 6 4 205 201 173.18 0.52 7 4 206 202 173.09 0.56 8 4 207 203 170.32 1.85 9 4 229 225 120.74 0.97 10 5 36 31 464.43 0.15 11 5 37 32 464.39 0.14 12 5 38 33 464.40 0.14 13 5 207 202 173.05 0.58 14 6 36 30 464.63 0.21 15 6 37 31 464.45 0.15 16 6 38 32 464.45 0.15 17 6 223 217 120.90 1.02 18 6 229 223 120.87 0.97 19 6 249 243 25.54 0.73 20 7 37 30 464.59 0.19 21 7 38 31 464.44 0.16 22 7 40 33 464.56 0.21 23 7 90 83 198.21 0.21 24 7 124 117 186.06 1.24 25 7 163 156 186.06 1.24 26 7 207 200 184.35 1.01 27 7 209 202 174.60 0.24 28 7 229 222 120.95 1.01 29 7 230 223 120.90 1.00 30 7 231 224 120.84 0.99 31 8 37 29 464.67 0.22 32 8 38 30 464.57 0.20 33 8 39 31 464.45 0.17 34 8 40 32 464.57 0.21 35 8 231 223 120.91 1.00 36 8 242 234 56.30 0.33 37 8 246 238 39.35 0.64 38 8 249 241 25.62 0.69 39 9 40 31 464.59 0.21 40 9 124 115 196.03 0.57 41 9 209 200 186.00 1.20 42 9 231 222 120.94 1.01 43 9 232 223 120.83 0.99 44 9 233 224 120.83 0.99 45 9 242 233 56.34 0.32 46 10 40 30 464.67 0.21 47 10 41 31 464.65 0.22 48 10 42 32 464.76 0.17 49 10 233 223 120.88 1.01 50 10 237 227 86.62 0.73 51 10 242 232 56.35 0.31 52 11 42 31 464.82 0.14 53 11 65 54 464.15 0.14 54 11 95 84 199.92 1.18 55 11 232 221 121.98 0.58 56 11 233 222 120.97 1.02 57 11 234 223 120.81 0.99 58 11 237 226 88.66 0.30 59 12 15 3 875.70 6.55 60 12 21 9 466.00 0.23 61 12 24 12 465.85 0.15 62 12 25 13 465.83 0.14 63 12 28 16 465.84 0.15 64 12 41 29 464.97 0.09 65 12 42 30 464.97 0.09 66 12 43 31 464.94 0.11 67 12 51 39 465.06 0.14 68 12 65 53 464.31 0.17 69 12 237 225 115.86 2.05 70 12 238 226 88.09 0.03 71 13 44 31 465.06 0.11 72 13 65 52 464.31 0.20 73 13 95 82 201.49 0.55 74 13 97 84 201.53 0.56 75 13 234 221 121.98 0.58 76 14 44 30 465.07 0.10 77 15 44 29 465.12 0.12 78 15 47 32 465.14 0.13 79 15 238 223 120.76 0.97 80 16 20 4 836.00 7.72 81 16 21 5 588.40 52.00 82 16 47 31 465.13 0.12 83 17 20 3 863.37 5.08 84 17 21 4 836.90 7.27 85 18 22 4 850.21 0.64 86 18 50 32 465.41 0.10 87 19 22 3 866.52 3.49 88 19 23 4 850.21 0.64 89 20 23 3 875.94 5.54 90 20 24 4 861.92 0.51 91 21 24 3 873.15 5.09 92 21 25 4 868.27 2.67 93 21 47 26 465.17 0.12 94 21 50 29 466.10 0.42 95 21 238 217 108.78 3.21 96 22 25 3 872.12 4.58 97 22 26 4 868.26 2.67 98 22 54 32 466.69 0.35 99 23 26 3 868.27 2.67 100 23 27 4 861.72 5.94 101 24 27 3 870.56 1.52 102 24 28 4 861.71 5.95 103 24 237 213 114.09 0.67 104 24 238 214 98.23 0.12 105 24 239 215 87.27 1.02 106 25 28 3 861.19 3.85 107 25 29 4 855.84 3.07 108 25 51 26 466.60 0.40 109 25 54 29 468.32 0.39 110 25 55 30 466.77 0.39 111 26 29 3 853.50 2.23 112 26 30 4 849.10 0.03 113 27 30 3 852.65 2.65 114 27 31 4 852.65 2.65 115 27 240 213 78.64 0.07 116 27 243 216 59.93 1.67 117 28 31 3 859.35 0.92 118 28 41 13 468.34 0.36 119 28 55 27 468.59 0.41 120 28 57 29 468.75 0.34 121 28 60 32 468.45 0.36 122 28 237 209 98.84 0.09 123 28 242 214 58.13 0.78 124 29 54 25 468.65 0.38 125 29 55 26 468.61 0.40 126 29 56 27 468.73 0.34 127 29 57 28 468.75 0.33 128 31 57 26 482.66 6.08 129 31 243 212 55.49 0.70 130 33 37 4 883.14 4.53 131 33 39 6 508.43 2.33 132 33 57 24 547.66 1.32 133 33 59 26 519.63 3.27 134 33 60 27 519.63 3.27 135 33 246 213 45.59 0.08 136 35 59 24 568.94 3.12 137 35 246 211 46.86 0.56 138 35 247 212 42.55 1.35 139 35 249 214 37.45 0.96 140 35 250 215 25.09 0.14 141 36 59 23 568.95 3.12 142 37 59 22 568.98 3.12 143 37 246 209 42.15 1.29 144 37 249 212 26.87 0.26 145 38 59 21 568.99 3.12 146 38 60 22 568.98 3.12 147 38 61 23 568.96 3.12 148 38 90 52 198.14 0.18 149 39 59 20 569.01 3.12 150 39 60 21 568.98 3.12 151 39 61 22 568.98 3.12 152 39 246 207 42.24 1.34 153 40 61 21 568.98 3.12 154 40 63 23 568.94 3.12 155 40 90 50 199.22 0.30 156 41 51 10 569.28 3.04 157 41 55 14 569.24 3.06 158 41 60 19 569.13 3.11 159 41 61 20 569.08 3.10 160 41 62 21 568.97 3.12 161 41 63 22 568.96 3.12 162 42 63 21 568.96 3.12 163 42 65 23 568.93 3.13 164 42 92 50 199.39 0.35 165 43 47 4 831.35 3.40 166 43 48 5 700.11 62.22 167 43 51 8 569.49 3.10 168 43 63 20 569.12 3.08 169 43 65 22 568.95 3.12 170 44 47 3 860.43 0.93 171 44 48 4 842.09 4.40 172 44 65 21 569.06 3.10 173 45 48 3 871.70 2.08 174 45 64 19 569.12 3.08 175 45 66 21 569.06 3.10 176 45 78 33 211.81 0.93 177 47 50 3 886.55 1.52 178 47 51 4 851.32 3.62 179 48 51 3 873.94 4.79 180 48 52 4 851.33 3.61 181 48 62 14 569.20 3.08 182 48 63 15 569.19 3.08 183 48 64 16 569.13 3.08 184 48 83 35 200.47 0.33 185 48 86 38 199.67 0.06 186 48 88 40 199.64 0.08 187 49 52 3 856.47 3.95 188 49 53 4 851.32 3.62 189 49 86 37 199.59 0.06 190 50 53 3 854.21 5.07 191 50 54 4 820.73 3.13 192 51 54 3 860.23 2.06 193 51 55 4 760.07 33.43 194 51 62 11 569.19 3.08 195 52 62 10 569.21 3.10 196 52 86 34 199.96 0.08 197 55 60 5 569.35 3.11 198 55 62 7 569.29 3.12 199 57 60 3 860.08 1.45 200 60 246 186 44.65 0.38 201 61 90 29 199.16 0.34 202 62 87 25 200.46 0.33 203 62 88 26 199.94 0.09 204 62 90 28 199.24 0.38 205 63 88 25 200.47 0.31 206 63 90 27 199.17 0.34 207 63 91 28 199.69 0.18 208 64 83 19 201.59 0.38 209 64 88 24 200.97 0.39 210 64 90 26 199.88 0.13 211 64 91 27 199.79 0.18 212 64 92 28 199.88 0.16 213 65 92 27 200.00 0.12 214 66 77 11 212.34 1.04 215 66 83 17 211.25 1.13 216 66 91 25 201.71 0.50 217 66 92 26 201.68 0.51 218 66 93 27 201.62 0.48 219 66 98 32 201.71 0.53 220 66 104 38 203.28 0.07 221 66 112 46 203.49 0.07 222 67 78 11 212.03 0.91 223 67 79 12 211.94 0.94 224 67 80 13 211.38 1.16 225 67 83 16 211.37 1.17 226 67 91 24 203.09 0.17 227 67 93 26 203.09 0.17 228 67 112 45 203.99 0.32 229 68 74 6 289.38 38.90 230 68 77 9 212.40 1.04 231 68 78 10 212.40 1.04 232 68 79 11 212.28 1.02 233 68 80 12 212.34 0.98 234 68 112 44 204.03 0.20 235 69 80 11 212.39 1.01 236 69 81 12 212.41 0.98 237 69 115 46 206.50 0.57 238 70 74 4 905.60 2.85 239 70 79 9 212.42 0.99 240 70 81 11 212.46 0.96 241 71 74 3 947.10 0.85 242 71 79 8 212.46 0.97 243 71 81 10 212.47 0.96 244 71 82 11 212.47 0.96 245 74 77 3 874.45 3.90 246 78 82 4 844.28 1.26 247 78 83 5 838.36 5.81 248 79 82 3 861.90 0.42 249 79 83 4 847.93 1.03 250 80 83 3 857.48 1.85 251 80 84 4 847.93 1.03 252 80 91 11 406.48 31.66 253 80 115 35 411.84 30.30 254 80 116 36 408.62 31.21 255 80 121 41 495.80 6.87 256 81 84 3 852.49 3.31 257 81 85 4 848.58 1.36 258 82 85 3 878.82 2.42 259 82 86 4 852.08 3.52 260 83 86 3 856.44 1.33 261 83 87 4 852.08 3.52 262 83 88 5 657.81 81.09 263 83 91 8 529.03 19.21 264 83 121 38 585.25 4.04 265 84 87 3 854.90 2.45 266 84 88 4 830.86 5.42 267 84 119 35 585.36 4.04 268 84 121 37 585.32 4.08 269 88 121 33 662.90 1.67 270 89 120 31 662.91 1.67 271 89 121 32 662.89 1.67 272 89 122 33 662.82 1.70 273 90 121 31 662.91 1.67 274 90 122 32 662.83 1.71 275 90 124 34 662.81 1.71 276 90 163 73 662.82 1.70 277 91 116 25 663.05 1.72 278 91 121 30 663.03 1.73 279 91 122 31 662.91 1.67 280 91 123 32 662.84 1.70 281 91 124 33 662.81 1.71 282 92 95 3 919.53 3.26 283 92 124 32 662.89 1.67 284 92 126 34 662.81 1.71 285 92 232 140 122.83 0.68 286 93 112 19 663.15 1.73 287 93 113 20 663.09 1.73 288 93 116 23 663.15 1.73 289 93 123 30 663.06 1.72 290 93 124 31 662.93 1.68 291 93 125 32 662.82 1.70 292 93 126 33 662.81 1.71 293 94 99 5 823.28 2.77 294 94 104 10 663.07 1.72 295 94 109 15 663.07 1.72 296 94 126 32 662.83 1.70 297 95 98 3 868.30 4.08 298 95 99 4 849.86 4.19 299 95 126 31 662.90 1.67 300 95 167 72 662.77 1.69 301 95 232 137 124.08 0.57 302 96 99 3 857.43 1.34 303 96 100 4 839.09 1.04 304 96 167 71 662.76 1.69 305 96 169 73 659.83 2.08 306 97 100 3 858.04 9.21 307 97 101 4 836.43 0.28 308 98 101 3 855.87 1.19 309 98 102 4 814.50 1.59 310 98 103 5 814.50 1.59 311 98 104 6 663.54 1.75 312 99 104 5 663.44 1.71 313 99 106 7 663.43 1.71 314 99 109 10 663.44 1.71 315 99 126 27 663.12 1.73 316 99 127 28 663.09 1.72 317 99 146 47 663.12 1.72 318 103 108 5 843.52 3.37 319 104 108 4 828.18 4.30 320 104 109 5 827.58 4.58 321 104 112 8 824.27 2.83 322 105 108 3 855.58 2.66 323 105 109 4 855.57 2.66 324 106 109 3 860.81 2.33 325 106 110 4 855.57 2.66 326 106 145 39 663.44 1.72 327 106 146 40 663.25 1.70 328 106 149 43 663.58 1.66 329 107 110 3 868.16 3.64 330 107 111 4 855.56 2.66 331 107 153 46 665.50 1.58 332 108 111 3 870.72 2.36 333 108 112 4 844.20 3.85 334 109 112 3 868.18 3.63 335 109 113 4 843.37 3.44 336 109 150 41 663.65 1.66 337 110 113 3 859.71 1.38 338 110 114 4 838.39 0.96 339 110 149 39 663.72 1.63 340 110 150 40 663.64 1.66 341 110 153 43 665.50 1.58 342 111 114 3 864.14 0.83 343 111 115 4 851.18 7.35 344 111 153 42 665.50 1.58 345 112 115 3 863.65 2.79 346 112 116 4 858.95 3.48 347 113 116 3 863.64 2.79 348 113 117 4 858.95 3.47 349 113 123 10 665.31 1.52 350 113 125 12 663.57 1.69 351 113 150 37 665.43 1.54 352 113 154 41 666.24 1.59 353 114 117 3 872.79 3.45 354 114 118 4 849.00 1.50 355 114 153 39 665.94 1.79 356 114 154 40 665.70 1.66 357 115 118 3 856.60 1.54 358 115 119 4 847.32 2.35 359 116 119 3 855.96 2.91 360 116 120 4 846.38 1.88 361 116 121 5 801.82 9.08 362 116 123 7 675.72 2.88 363 117 120 3 854.19 3.79 364 117 121 4 836.41 3.08 365 117 123 6 743.42 18.13 366 117 154 37 703.88 1.70 367 117 158 41 703.89 1.69 368 117 161 44 703.88 1.66 369 117 162 45 703.88 1.66 370 120 161 41 785.51 3.41 371 121 161 40 785.52 3.41 372 122 160 38 785.61 3.39 373 122 161 39 785.52 3.42 374 122 207 85 300.97 0.07 375 123 161 38 785.60 3.39 376 123 162 39 785.53 3.41 377 123 163 40 785.52 3.41 378 124 163 39 785.53 3.41 379 124 165 41 785.47 3.41 380 124 209 85 300.52 0.21 381 124 232 108 122.84 0.67 382 125 150 25 785.84 3.35 383 125 163 38 785.59 3.40 384 125 164 39 785.53 3.41 385 125 165 40 785.53 3.41 386 126 165 39 785.53 3.41 387 126 167 41 785.51 3.41 388 126 232 106 123.98 0.61 389 127 143 16 786.09 3.29 390 127 146 19 786.00 3.31 391 127 147 20 785.95 3.35 392 127 150 23 785.98 3.33 393 127 164 37 785.63 3.39 394 127 165 38 785.60 3.40 395 127 166 39 785.53 3.42 396 127 167 40 785.53 3.41 397 127 190 63 307.85 1.44 398 128 143 15 786.11 3.29 399 128 146 18 786.07 3.29 400 128 166 38 785.58 3.40 401 128 167 39 785.53 3.42 402 129 133 4 831.87 4.41 403 129 134 5 830.74 4.98 404 129 139 10 786.30 3.34 405 129 142 13 786.25 3.35 406 129 143 14 786.24 3.35 407 129 166 37 785.64 3.39 408 129 169 40 785.50 3.42 409 129 170 41 785.46 3.43 410 130 133 3 865.96 2.12 411 130 134 4 839.41 0.64 412 130 169 39 785.50 3.43 413 130 170 40 785.43 3.42 414 131 134 3 865.43 0.07 415 131 135 4 842.34 0.82 416 131 170 39 785.45 3.42 417 131 174 43 732.21 2.73 418 131 176 45 698.55 0.37 419 132 135 3 865.58 2.66 420 132 136 4 839.47 0.61 421 133 136 3 862.13 1.57 422 133 137 4 846.51 2.10 423 133 138 5 786.36 3.34 424 133 142 9 786.35 3.34 425 134 137 3 846.06 1.88 426 134 139 5 786.34 3.35 427 134 166 32 785.75 3.37 428 134 170 36 785.49 3.40 429 134 182 48 321.04 7.13 430 138 141 3 856.34 2.44 431 138 142 4 818.83 2.24 432 139 142 3 867.99 2.13 433 139 143 4 831.63 4.66 434 140 144 4 839.13 0.06 435 140 189 49 308.18 1.41 436 141 144 3 869.33 1.94 437 141 145 4 842.66 0.83 438 142 145 3 860.29 0.77 439 142 146 4 842.66 0.83 440 143 146 3 859.75 0.49 441 143 147 4 848.55 1.62 442 143 166 23 785.70 3.37 443 143 190 47 308.00 1.44 444 144 147 3 863.78 1.81 445 144 148 4 848.55 1.63 446 144 189 45 308.13 1.41 447 144 190 46 308.01 1.45 448 144 193 49 307.77 1.44 449 145 148 3 856.22 1.98 450 145 149 4 846.95 2.65 451 146 149 3 856.27 2.00 452 146 150 4 854.77 1.27 453 147 150 3 854.57 1.38 454 147 151 4 858.37 7.08 455 147 159 12 786.45 3.33 456 147 164 17 785.70 3.37 457 147 190 43 307.88 1.42 458 147 193 46 307.76 1.44 459 147 194 47 307.72 1.46 460 148 151 3 866.38 3.08 461 148 152 4 856.86 7.84 462 148 193 45 307.76 1.44 463 149 152 3 881.90 4.68 464 149 153 4 866.38 3.08 465 150 153 3 885.00 1.55 466 150 154 4 866.38 3.08 467 151 154 3 864.06 4.25 468 151 159 8 786.48 3.33 469 151 193 42 307.75 1.44 470 151 198 47 302.39 0.07 471 154 158 4 854.87 5.38 472 154 159 5 848.22 2.06 473 154 162 8 786.36 3.27 474 155 158 3 871.51 0.72 475 155 159 4 871.51 0.72 476 156 159 3 887.63 2.23 477 156 201 45 302.22 0.12 478 156 202 46 302.07 0.19 479 157 160 3 891.78 1.13 480 158 161 3 876.80 2.12 481 158 162 4 859.13 2.23 482 159 162 3 852.19 5.71 483 159 194 35 307.38 1.47 484 159 198 39 302.31 0.10 485 159 202 43 301.69 0.12 486 159 206 47 301.06 0.10 487 162 206 44 301.05 0.10 488 163 206 43 301.09 0.11 489 163 207 44 300.97 0.07 490 163 209 46 300.53 0.21 491 164 190 26 307.74 1.44 492 164 206 42 301.14 0.12 493 164 207 43 301.00 0.08 494 164 208 44 300.53 0.21 495 164 209 45 300.53 0.21 496 165 209 44 300.52 0.21 497 165 232 67 123.98 0.60 498 166 186 20 307.88 1.43 499 166 187 21 307.88 1.43 500 166 190 24 307.75 1.44 501 166 208 42 300.99 0.09 502 166 209 43 300.65 0.15 503 166 210 44 300.53 0.21 504 166 211 45 300.05 0.43 505 167 211 44 299.50 0.23 506 168 171 3 870.52 1.43 507 168 172 4 862.31 1.92 508 168 210 42 300.63 0.16 509 168 211 43 300.50 0.22 510 168 212 44 299.54 0.18 511 169 172 3 872.85 0.27 512 169 174 5 844.92 4.11 513 170 174 4 851.13 3.66 514 170 176 6 607.97 42.27 515 170 177 7 588.07 51.38 516 170 178 8 358.80 2.19 517 170 182 12 320.65 6.95 518 171 174 3 875.71 1.50 519 171 176 5 499.92 11.76 520 171 178 7 320.60 6.92 521 172 211 39 300.63 0.16 522 172 212 40 299.94 0.36 523 172 213 41 299.56 0.18 524 177 182 5 846.39 1.77 525 178 182 4 838.47 2.19 526 178 210 32 301.14 0.16 527 179 182 3 860.05 3.71 528 179 183 4 846.40 1.77 529 180 183 3 849.06 0.45 530 180 184 4 846.43 1.76 531 180 221 41 169.67 22.29 532 180 222 42 127.99 1.46 533 180 225 45 127.29 1.26 534 181 184 3 853.75 2.79 535 181 185 4 856.73 6.90 536 182 185 3 867.66 1.44 537 182 186 4 856.73 6.88 538 183 186 3 870.94 5.67 539 183 187 4 862.00 4.26 540 183 210 27 301.17 0.14 541 183 222 39 127.95 1.46 542 184 187 3 862.00 4.26 543 184 188 4 861.99 4.26 544 184 225 41 125.72 0.61 545 184 226 42 125.74 0.62 546 184 227 43 125.54 0.62 547 185 188 3 862.00 4.26 548 185 189 4 861.99 4.26 549 186 189 3 861.98 4.25 550 186 190 4 857.00 6.74 551 187 190 3 859.94 5.25 552 187 191 4 858.98 5.73 553 187 208 21 301.45 0.12 554 187 209 22 301.44 0.12 555 187 210 23 301.35 0.10 556 187 227 40 125.37 0.58 557 187 228 41 125.33 0.58 558 188 191 3 866.62 5.65 559 188 192 4 846.23 0.65 560 188 227 39 125.39 0.58 561 189 192 3 866.61 5.66 562 189 193 4 840.07 3.73 563 190 193 3 866.61 5.66 564 190 194 4 843.16 2.22 565 190 208 18 301.43 0.11 566 191 194 3 852.16 1.39 567 191 195 4 841.19 3.06 568 191 203 12 302.44 0.07 569 191 206 15 301.88 0.09 570 191 208 17 301.45 0.12 571 191 227 36 125.28 0.61 572 191 229 38 125.30 0.62 573 192 195 3 861.38 5.71 574 192 196 4 841.96 2.82 575 193 196 3 863.90 4.45 576 193 197 4 841.17 3.05 577 193 198 5 841.33 3.13 578 194 197 3 866.65 3.07 579 194 198 4 828.30 1.09 580 194 199 5 826.25 2.08 581 194 202 8 359.08 16.12 582 194 203 9 318.35 3.23 583 194 206 12 318.33 3.23 584 195 199 4 807.45 4.83 585 195 200 5 354.93 8.29 586 195 203 8 319.80 2.48 587 198 202 4 847.54 0.06 588 199 202 3 871.60 3.16 589 199 203 4 848.85 0.70 590 200 203 3 848.85 0.71 591 200 204 4 848.85 0.70 592 201 204 3 856.09 1.73 593 201 205 4 852.35 2.46 594 202 205 3 853.01 2.12 595 202 206 4 839.14 2.10 596 203 206 3 853.02 2.12 597 206 229 23 124.78 0.73 598 207 229 22 124.62 0.68 599 208 227 19 125.17 0.58 600 208 228 20 125.12 0.60 601 208 229 21 124.79 0.68 602 209 229 20 124.75 0.62 603 209 230 21 124.62 0.64 604 209 231 22 124.37 0.70 605 209 232 23 124.31 0.68 606 210 214 4 872.32 1.84 607 210 222 12 125.48 0.71 608 210 228 18 125.17 0.58 609 210 230 20 124.90 0.53 610 210 231 21 124.53 0.66 611 210 232 22 124.41 0.68 612 211 232 21 124.34 0.69 613 213 218 5 819.27 1.89 614 214 218 4 817.88 1.20 615 214 219 5 465.23 59.30 616 214 222 8 352.67 3.02 617 214 231 17 124.93 0.48 618 214 233 19 124.65 0.60 619 214 236 22 124.06 0.58 620 214 245 31 54.30 0.35 621 215 218 3 862.58 4.59 622 215 219 4 815.20 4.24 623 215 236 21 124.06 0.58 624 215 241 26 57.80 0.96 625 215 245 30 54.52 0.33 626 216 241 25 56.43 0.27 627 216 244 28 54.99 0.44 628 216 245 29 54.85 0.40 629 217 241 24 59.58 1.82 630 218 221 3 868.30 1.94 631 218 222 4 848.84 7.79 632 219 222 3 855.18 4.62 633 219 223 4 852.27 6.07 634 219 231 12 125.41 0.43 635 219 245 26 44.87 0.45 636 219 248 29 35.09 0.86 637 220 223 3 860.16 9.77 638 220 224 4 862.30 8.71 639 220 248 28 34.39 0.59 640 220 250 30 34.38 0.59 641 221 224 3 888.32 6.13 642 221 225 4 873.14 3.31 643 222 225 3 889.23 3.79 644 222 228 6 313.02 1.99 645 222 231 9 125.63 0.54 646 223 228 5 314.23 2.58 647 223 229 6 220.00 44.53 648 223 230 7 125.72 0.52 649 223 231 8 125.72 0.52 650 223 248 25 34.37 0.60 651 223 249 26 29.45 0.86 652 223 250 27 29.63 0.78 653 225 228 3 874.37 4.17 654 228 231 3 1035.87 3.74 655 230 249 19 24.71 0.33 656 231 245 14 42.81 1.47 657 231 249 18 25.61 0.70 658 233 236 3 890.81 1.18 659 233 237 4 825.07 9.32 660 233 242 9 127.03 0.38 661 233 245 12 46.62 0.43 662 234 237 3 876.61 3.14 663 235 238 3 880.12 1.38 664 235 239 4 874.34 4.27 665 236 239 3 867.75 0.97 666 236 241 5 360.06 5.46 667 236 242 6 337.68 10.18 668 236 245 9 270.83 23.24 669 237 242 5 357.22 0.43 670 239 242 3 874.92 4.57 671 240 243 3 873.37 3.68 672 241 244 3 866.88 0.37 673 241 245 4 843.83 1.27 674 242 245 3 854.46 6.58 675 242 246 4 843.87 1.29 676 243 246 3 856.30 4.92 677 243 247 4 851.22 2.38 678 244 247 3 857.03 0.48 679 244 248 4 851.23 2.38 680 245 248 3 857.00 0.51 681 245 249 4 861.38 2.70 682 246 249 3 877.00 3.96 683 247 250 3 877.00 4.80