BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 93 92 34.68 0.88 2 2 30 28 32.48 0.51 3 2 60 58 47.17 0.97 4 2 61 59 47.16 0.97 5 2 62 60 42.86 0.18 6 2 66 64 35.33 0.94 7 2 92 90 46.77 0.79 8 2 93 91 43.06 0.12 9 3 59 56 50.28 0.05 10 3 60 57 48.63 0.53 11 3 61 58 48.86 0.42 12 3 90 87 53.70 0.34 13 3 91 88 49.01 0.58 14 3 92 89 47.45 1.10 15 4 30 26 48.59 0.55 16 4 35 31 47.11 0.95 17 4 57 53 49.37 0.48 18 4 59 55 50.30 0.04 19 4 60 56 48.86 0.42 20 4 90 86 54.79 0.03 21 4 91 87 49.30 0.53 22 5 57 52 51.96 0.79 23 5 58 53 53.95 0.64 24 5 59 54 52.38 0.99 25 5 88 83 56.34 0.39 26 5 89 84 56.23 0.42 27 5 90 85 55.76 0.46 28 6 12 6 765.60 23.05 29 6 35 29 52.13 0.76 30 6 57 51 53.65 0.49 31 6 88 82 56.48 0.45 32 6 89 83 56.26 0.43 33 6 91 85 56.34 0.39 34 7 56 49 56.26 0.44 35 7 57 50 56.26 0.44 36 7 58 51 56.27 0.44 37 7 87 80 86.56 0.05 38 8 11 3 836.27 0.91 39 8 12 4 832.82 0.15 40 8 237 229 55.98 0.48 41 9 12 3 832.82 0.16 42 9 13 4 826.21 3.15 43 9 87 78 92.06 1.95 44 9 89 80 86.45 0.09 45 10 13 3 840.36 1.38 46 10 14 4 820.14 0.82 47 10 83 73 96.96 0.78 48 10 84 74 96.97 0.78 49 10 214 204 56.37 0.39 50 11 14 3 845.56 1.23 51 11 15 4 822.81 2.12 52 11 235 224 57.87 0.36 53 11 237 226 63.26 0.35 54 12 15 3 849.27 3.56 55 12 16 4 825.96 2.52 56 12 78 66 97.75 0.59 57 12 89 77 97.22 0.63 58 12 91 79 97.12 0.60 59 13 16 3 851.25 2.56 60 13 17 4 823.98 1.54 61 13 78 65 97.75 0.59 62 13 79 66 97.81 0.66 63 13 81 68 98.19 0.48 64 13 82 69 98.15 0.48 65 13 83 70 98.18 0.49 66 13 89 76 97.52 0.54 67 13 102 89 97.72 0.58 68 14 17 3 836.58 2.80 69 14 18 4 825.94 2.52 70 14 214 200 69.39 0.37 71 14 215 201 70.55 0.42 72 15 18 3 834.92 1.98 73 15 19 4 834.92 1.98 74 15 50 35 71.45 0.05 75 15 52 37 76.15 1.76 76 15 215 200 73.22 0.74 77 15 235 220 75.14 1.60 78 15 239 224 76.09 1.77 79 15 240 225 75.17 1.64 80 15 241 226 71.42 0.06 81 16 19 3 841.60 1.37 82 16 20 4 833.23 2.81 83 16 26 10 355.26 71.36 84 16 75 59 98.09 0.49 85 16 78 62 98.16 0.48 86 16 79 63 98.17 0.48 87 17 20 3 841.60 1.37 88 17 21 4 846.54 1.10 89 17 79 62 105.70 3.19 90 17 213 196 76.05 1.80 91 18 21 3 862.69 2.19 92 18 211 193 75.14 1.50 93 18 215 197 76.11 1.79 94 18 241 223 76.08 1.80 95 18 243 225 75.36 1.45 96 19 22 3 854.74 1.68 97 19 23 4 831.69 2.67 98 19 26 7 369.72 68.06 99 19 37 18 200.78 3.15 100 19 39 20 254.75 10.56 101 19 48 29 201.25 3.73 102 19 50 31 137.44 32.34 103 19 241 222 76.23 1.75 104 20 23 3 837.55 5.59 105 20 75 55 120.03 2.00 106 20 79 59 177.39 0.28 107 21 207 186 75.13 1.50 108 21 210 189 76.06 1.80 109 21 211 190 76.06 1.80 110 22 39 17 285.68 1.23 111 22 40 18 285.70 1.24 112 22 41 19 294.87 3.35 113 22 46 24 300.66 6.24 114 22 47 25 285.69 1.23 115 22 48 26 281.52 0.84 116 23 39 16 294.86 3.36 117 24 72 48 183.34 1.10 118 24 75 51 183.27 1.07 119 25 39 14 306.49 6.79 120 25 69 44 184.22 0.93 121 25 70 45 183.66 1.19 122 25 71 46 183.38 1.11 123 25 72 47 183.37 1.11 124 25 75 50 183.27 1.07 125 26 37 11 307.12 6.48 126 26 39 13 306.50 6.79 127 26 69 43 184.22 0.94 128 26 70 44 183.69 1.11 129 26 75 49 183.40 1.06 130 27 37 10 307.12 6.48 131 27 39 12 307.12 6.49 132 27 67 40 184.44 0.97 133 27 68 41 184.35 0.94 134 27 69 42 184.22 0.94 135 27 123 96 142.86 12.83 136 28 35 7 541.36 1.49 137 28 37 9 307.12 6.48 138 28 50 22 306.50 6.78 139 28 67 39 184.44 0.97 140 28 68 40 184.35 0.94 141 28 70 42 184.34 0.94 142 29 34 5 734.85 3.09 143 29 35 6 541.37 1.49 144 29 36 7 417.06 60.65 145 29 66 37 185.00 0.98 146 29 67 38 184.44 0.97 147 29 122 93 177.10 0.42 148 29 123 94 175.76 0.12 149 30 34 4 674.72 26.98 150 30 35 5 555.19 3.28 151 30 60 30 212.06 0.58 152 30 62 32 202.49 0.04 153 30 65 35 185.16 0.94 154 30 66 36 185.06 0.95 155 30 68 38 184.93 0.99 156 30 91 61 181.60 1.27 157 30 122 92 179.83 0.85 158 31 34 3 833.30 2.80 159 31 36 5 634.28 7.42 160 31 60 29 229.41 0.58 161 31 62 31 209.88 0.43 162 31 65 34 185.16 0.94 163 31 122 91 180.41 1.14 164 31 124 93 179.38 0.73 165 32 54 22 306.37 6.77 166 32 62 30 209.89 0.43 167 32 65 33 189.90 3.27 168 33 53 20 306.43 6.75 169 33 54 21 306.13 6.66 170 34 52 18 306.50 6.78 171 34 53 19 306.09 6.64 172 34 54 20 305.94 6.62 173 34 60 26 221.89 4.33 174 35 50 15 306.58 6.81 175 35 51 16 306.53 6.83 176 35 52 17 306.38 6.78 177 35 57 22 298.36 5.20 178 35 60 25 221.88 4.33 179 36 50 14 306.54 6.83 180 36 51 15 306.53 6.83 181 36 123 87 180.49 1.14 182 36 124 88 180.41 1.15 183 36 125 89 179.65 1.10 184 37 49 12 306.67 6.79 185 37 50 13 306.55 6.83 186 38 47 9 307.02 6.64 187 38 48 10 306.92 6.68 188 38 49 11 306.68 6.79 189 38 125 87 180.43 1.14 190 38 126 88 180.41 1.15 191 39 47 8 307.02 6.64 192 39 48 9 306.94 6.67 193 40 44 4 754.22 22.00 194 40 46 6 515.10 8.64 195 40 47 7 307.04 6.63 196 41 44 3 823.68 2.25 197 41 45 4 769.59 14.31 198 41 46 5 536.08 5.83 199 41 204 163 159.68 0.90 200 45 204 159 160.16 1.05 201 45 245 200 160.02 1.03 202 45 246 201 159.35 0.73 203 46 204 158 159.39 0.74 204 46 207 161 160.16 1.04 205 46 208 162 160.18 1.05 206 46 243 197 160.21 1.04 207 46 244 198 160.03 1.02 208 46 245 199 159.35 0.73 209 47 128 81 179.75 1.12 210 47 243 196 160.18 1.03 211 47 244 197 159.42 0.73 212 48 242 194 160.19 1.02 213 48 243 195 159.42 0.72 214 49 125 76 180.46 1.12 215 49 127 78 179.75 1.34 216 49 241 192 160.21 1.02 217 49 242 193 159.42 0.73 218 49 244 195 159.37 0.73 219 50 240 190 160.21 1.02 220 50 241 191 159.96 0.99 221 51 125 74 180.12 1.28 222 51 127 76 179.35 1.23 223 51 239 188 160.20 1.03 224 51 240 189 159.82 0.91 225 51 242 191 159.41 0.72 226 52 56 4 838.82 6.46 227 52 57 5 812.75 4.58 228 52 239 187 159.81 0.91 229 53 56 3 869.20 0.30 230 53 239 186 159.89 0.92 231 54 57 3 846.49 2.63 232 54 60 6 596.85 22.15 233 56 237 181 160.27 1.03 234 56 238 182 160.19 1.05 235 56 239 183 160.15 1.07 236 57 60 3 854.53 1.97 237 62 65 3 886.24 1.02 238 65 122 57 179.75 0.89 239 66 93 27 181.38 1.24 240 66 120 54 180.44 1.14 241 66 122 56 179.22 0.62 242 67 119 52 180.59 1.20 243 67 120 53 180.28 1.07 244 67 121 54 179.31 0.61 245 67 122 55 176.44 0.09 246 68 93 25 181.28 1.23 247 68 98 30 181.19 1.25 248 68 118 50 180.66 1.20 249 68 119 51 180.04 0.97 250 69 117 48 180.62 1.22 251 69 118 49 180.57 1.22 252 70 74 4 814.98 5.61 253 70 75 5 801.31 1.94 254 70 78 8 751.50 22.96 255 70 98 28 181.13 1.24 256 70 115 45 180.99 1.21 257 70 117 47 180.60 1.22 258 71 74 3 834.48 0.29 259 71 75 4 824.30 1.01 260 72 75 3 842.15 3.55 261 72 76 4 827.91 0.80 262 73 76 3 844.90 3.50 263 73 77 4 827.90 0.79 264 74 77 3 827.64 0.70 265 74 78 4 824.30 0.97 266 74 115 41 180.99 1.21 267 74 116 42 180.78 1.30 268 75 78 3 837.06 7.88 269 75 79 4 829.57 2.79 270 76 79 3 832.99 1.08 271 76 80 4 829.57 2.79 272 77 80 3 840.30 1.78 273 77 113 36 181.23 1.27 274 77 114 37 181.23 1.27 275 77 115 38 181.01 1.19 276 78 81 3 837.89 0.58 277 78 82 4 832.99 1.08 278 78 100 22 181.28 1.29 279 78 113 35 181.24 1.27 280 78 115 37 181.09 1.22 281 79 82 3 826.30 2.27 282 81 102 21 181.47 1.29 283 81 113 32 181.28 1.28 284 86 104 18 797.84 0.94 285 86 105 19 797.81 0.94 286 87 102 15 797.98 0.94 287 87 103 16 797.86 0.94 288 87 104 17 797.84 0.94 289 87 105 18 797.84 0.94 290 88 101 13 798.30 0.88 291 88 102 14 797.98 0.94 292 88 103 15 797.90 0.96 293 89 100 11 798.38 0.86 294 89 101 12 798.30 0.89 295 89 102 13 798.18 0.90 296 90 99 9 798.52 0.82 297 90 100 10 798.39 0.85 298 90 101 11 798.32 0.88 299 91 98 7 798.65 0.82 300 91 99 8 798.52 0.82 301 91 100 9 798.39 0.85 302 92 98 6 798.65 0.82 303 92 99 7 798.54 0.81 304 93 97 4 798.72 0.81 305 93 98 5 798.65 0.83 306 93 119 26 774.47 4.46 307 94 97 3 817.25 4.09 308 94 98 4 798.70 0.82 309 94 99 5 798.70 0.82 310 94 119 25 796.88 1.05 311 95 119 24 797.00 1.06 312 96 116 20 797.43 0.96 313 96 117 21 797.40 0.96 314 96 119 23 797.05 1.04 315 97 115 18 797.57 0.94 316 97 116 19 797.13 1.08 317 97 117 20 796.83 1.03 318 97 119 22 795.68 1.61 319 98 114 16 797.57 0.94 320 98 115 17 797.06 1.04 321 99 112 13 797.80 0.94 322 99 113 14 797.60 0.94 323 99 114 15 797.23 1.02 324 100 112 12 797.62 0.91 325 100 113 13 797.50 0.91 326 100 115 15 797.01 1.05 327 101 110 9 797.81 0.94 328 101 111 10 797.65 0.91 329 101 112 11 797.46 0.98 330 102 110 8 797.65 0.91 331 102 111 9 797.60 0.92 332 102 113 11 797.45 0.98 333 103 108 5 797.83 0.95 334 103 109 6 797.75 0.94 335 103 110 7 797.58 0.92 336 104 107 3 884.14 2.08 337 104 108 4 797.74 0.93 338 104 109 5 797.65 0.91 339 127 242 115 292.92 26.25 340 128 244 116 205.94 5.86 341 130 227 97 354.04 5.76 342 132 222 90 361.04 5.00 343 132 223 91 360.53 5.09 344 132 224 92 360.30 4.79 345 132 227 95 360.11 4.88 346 132 228 96 360.29 4.79 347 132 230 98 361.06 5.01 348 132 244 112 361.04 5.00 349 133 193 60 369.88 5.05 350 133 194 61 368.51 5.47 351 133 223 90 365.33 3.87 352 134 161 27 369.63 4.92 353 134 166 32 389.90 3.75 354 134 191 57 390.56 3.77 355 134 194 60 371.26 4.61 356 134 196 62 365.33 3.87 357 134 222 88 367.62 2.79 358 134 223 89 365.33 3.87 359 135 190 55 412.27 1.51 360 135 191 56 403.31 2.43 361 135 220 85 390.75 3.86 362 135 221 86 389.97 4.03 363 135 222 87 374.50 0.21 364 136 159 23 390.74 3.86 365 136 166 30 412.27 1.51 366 136 181 45 427.63 0.22 367 136 187 51 429.57 0.38 368 136 189 53 428.86 0.23 369 136 190 54 416.26 0.48 370 136 220 84 390.77 3.87 371 136 221 85 390.43 3.88 372 137 187 50 429.59 0.39 373 137 188 51 429.58 0.38 374 137 189 52 429.31 0.35 375 137 218 81 429.50 0.39 376 137 219 82 424.18 1.70 377 137 220 83 401.72 1.58 378 138 142 4 821.94 2.62 379 138 143 5 820.32 1.82 380 138 186 48 432.69 1.13 381 138 187 49 429.60 0.38 382 138 188 50 431.92 1.48 383 138 218 80 429.54 0.39 384 138 219 81 429.47 0.41 385 139 142 3 856.69 8.56 386 139 143 4 827.66 3.10 387 139 188 49 432.92 1.07 388 139 218 79 429.54 0.39 389 139 219 80 429.50 0.41 390 140 143 3 847.97 7.05 391 140 144 4 824.31 1.42 392 140 212 72 429.56 0.39 393 141 144 3 848.15 6.96 394 141 145 4 824.34 1.43 395 142 145 3 848.15 6.96 396 142 146 4 824.34 1.45 397 142 181 39 445.70 5.08 398 142 186 44 447.45 5.96 399 143 146 3 830.39 2.37 400 143 147 4 821.81 2.66 401 143 205 62 433.63 0.70 402 143 209 66 433.64 0.69 403 143 212 69 434.72 0.30 404 143 219 76 433.71 0.70 405 143 221 78 433.69 0.69 406 144 147 3 837.39 1.11 407 144 148 4 834.75 3.76 408 144 209 65 433.71 0.71 409 145 148 3 839.45 2.14 410 145 149 4 819.91 1.88 411 146 149 3 832.95 4.67 412 146 150 4 824.07 0.21 413 146 168 22 459.56 3.48 414 146 181 35 459.98 3.50 415 146 205 59 435.32 0.21 416 147 150 3 856.48 0.95 417 147 151 4 822.50 0.56 418 147 205 58 435.32 0.21 419 147 206 59 435.32 0.21 420 147 209 62 435.35 0.20 421 148 151 3 852.08 3.15 422 148 152 4 823.25 0.93 423 149 152 3 844.88 1.31 424 149 153 4 836.98 4.41 425 149 177 28 537.55 6.73 426 150 153 3 839.25 0.72 427 150 154 4 814.69 0.62 428 150 157 7 561.39 5.15 429 150 168 18 524.56 1.87 430 150 170 20 537.64 6.70 431 150 177 27 535.94 7.54 432 150 202 52 435.87 0.04 433 151 154 3 833.99 4.16 434 151 206 55 436.73 0.15 435 153 170 17 631.31 10.54 436 153 171 18 631.31 10.54 437 153 175 22 631.31 10.54 438 153 176 23 631.30 10.54 439 153 177 24 628.10 12.13 440 154 171 17 632.60 9.89 441 155 201 46 466.04 2.11 442 155 202 47 465.93 2.14 443 155 248 93 440.38 1.79 444 156 170 14 647.88 13.91 445 156 199 43 466.82 2.38 446 156 200 44 466.73 2.38 447 156 202 46 466.09 2.14 448 156 248 92 440.37 1.79 449 157 168 11 648.07 13.90 450 157 169 12 648.00 13.89 451 157 199 42 466.82 2.38 452 157 200 43 466.77 2.35 453 157 202 45 466.27 2.17 454 157 205 48 466.11 2.14 455 157 229 72 466.67 2.38 456 158 168 10 648.08 13.91 457 158 169 11 648.01 13.90 458 158 170 12 648.00 13.89 459 158 171 13 648.01 13.89 460 158 198 40 467.14 2.27 461 158 199 41 466.83 2.38 462 159 166 7 648.24 13.91 463 159 167 8 648.13 13.89 464 159 168 9 648.08 13.91 465 159 197 38 467.35 2.32 466 159 198 39 467.25 2.27 467 159 200 41 467.25 2.27 468 159 223 64 466.63 2.39 469 159 229 70 466.63 2.39 470 160 166 6 648.24 13.91 471 160 167 7 648.15 13.89 472 160 169 9 648.12 13.89 473 160 196 36 467.37 2.32 474 160 197 37 467.36 2.32 475 161 192 31 516.75 3.25 476 161 195 34 467.45 2.31 477 161 196 35 467.39 2.31 478 162 165 3 771.86 23.82 479 162 167 5 648.27 13.89 480 162 180 18 647.69 13.78 481 163 192 29 527.71 1.74 482 164 182 18 647.60 13.79 483 164 190 26 585.78 4.14 484 164 192 28 529.25 2.66 485 165 181 16 647.61 13.79 486 165 182 17 647.54 13.77 487 165 190 25 580.31 6.88 488 166 179 13 647.83 13.86 489 166 180 14 647.64 13.80 490 166 181 15 647.57 13.79 491 166 190 24 561.85 0.30 492 166 223 57 467.26 2.31 493 167 178 11 647.85 13.86 494 167 179 12 647.77 13.86 495 167 180 13 647.62 13.79 496 168 178 10 647.80 13.86 497 168 179 11 647.69 13.84 498 168 181 13 647.57 13.79 499 169 177 8 647.83 13.88 500 169 178 9 647.76 13.88 501 170 176 6 647.91 13.86 502 170 177 7 647.85 13.87 503 171 175 4 647.97 13.86 504 171 176 5 647.91 13.85 505 181 185 4 767.71 24.93 506 181 186 5 737.97 39.80 507 181 187 6 669.68 5.92 508 182 185 3 834.78 6.18 509 182 186 4 767.71 24.93 510 182 187 5 737.97 39.80 511 183 187 4 737.98 39.80 512 183 188 5 737.97 39.80 513 183 189 6 667.90 5.04 514 184 187 3 781.00 21.53 515 187 190 3 879.24 0.53 516 188 218 30 463.22 0.95 517 196 223 27 473.22 1.88 518 196 224 28 473.23 1.89 519 196 225 29 473.91 1.54 520 197 225 28 476.09 2.38 521 197 226 29 478.35 3.50 522 198 224 26 478.33 3.50 523 198 226 28 478.35 3.49 524 198 227 29 478.35 3.50 525 198 247 49 441.17 2.02 526 199 247 48 440.45 1.79 527 199 248 49 440.39 1.80 528 200 223 23 481.44 3.36 529 200 227 27 481.49 3.37 530 200 228 28 481.44 3.35 531 200 229 29 481.37 3.32 532 200 245 45 443.45 0.94 533 200 247 47 440.45 1.79 534 200 248 48 440.39 1.80 535 201 204 3 826.10 0.05 536 201 205 4 756.56 7.60 537 201 248 47 440.39 1.79 538 202 205 3 769.91 16.54 539 202 206 4 790.06 24.35 540 203 206 3 834.27 2.25 541 203 207 4 790.06 24.35 542 204 207 3 816.85 0.70 543 204 208 4 776.72 16.16 544 204 245 41 443.63 0.85 545 205 208 3 834.27 2.25 546 205 209 4 766.73 27.50 547 205 229 24 481.66 3.44 548 205 231 26 481.43 3.33 549 205 245 40 443.50 0.94 550 206 209 3 830.59 4.09 551 206 210 4 822.09 0.18 552 207 210 3 822.09 0.18 553 207 211 4 825.90 2.09 554 208 211 3 825.90 2.09 555 208 212 4 837.33 0.88 556 208 231 23 481.65 3.46 557 208 243 35 444.94 1.02 558 208 245 37 444.50 1.09 559 209 212 3 847.44 4.18 560 210 213 3 840.75 1.03 561 211 215 4 874.13 1.38 562 211 243 32 445.13 1.01 563 212 215 3 844.75 4.90 564 212 219 7 492.35 5.16 565 212 231 19 482.51 3.30 566 212 233 21 481.97 3.58 567 215 233 18 482.62 3.31 568 215 235 20 481.62 3.45 569 215 241 26 470.94 3.04 570 216 236 20 481.64 3.46 571 217 233 16 483.56 3.42 572 217 234 17 482.70 3.27 573 218 233 15 483.57 3.41 574 219 231 12 487.47 4.51 575 219 232 13 485.67 3.63 576 219 233 14 483.56 3.42 577 220 230 10 488.00 4.68 578 220 231 11 487.49 4.50 579 220 232 12 487.44 4.51 580 221 229 8 488.09 4.69 581 221 230 9 488.00 4.69 582 221 231 10 487.49 4.50 583 222 229 7 488.09 4.70 584 222 230 8 488.06 4.69 585 223 228 5 496.06 2.44 586 223 229 6 488.09 4.70 587 224 227 3 686.54 6.65 588 226 247 21 443.45 0.96 589 226 249 23 441.00 2.10 590 227 247 20 443.47 0.95 591 228 244 16 444.56 0.82 592 228 245 17 444.20 0.95 593 228 246 18 443.50 0.94 594 228 247 19 443.44 0.94 595 229 244 15 444.53 0.83 596 229 245 16 444.47 0.82 597 230 242 12 445.13 0.94 598 230 243 13 445.05 0.97 599 230 244 14 444.51 0.83 600 231 242 11 445.14 0.95 601 231 243 12 445.11 0.95 602 231 245 14 445.01 0.98 603 232 240 8 489.20 4.57 604 232 241 9 489.15 4.57 605 232 242 10 445.69 0.68 606 233 240 7 489.20 4.57 607 233 241 8 489.18 4.56 608 234 238 4 757.19 25.04 609 234 239 5 562.50 3.30 610 234 240 6 489.20 4.57 611 235 238 3 816.31 5.98 612 235 239 4 573.87 2.38