BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 672 675 3 846.93 3.75 2 672 676 4 825.58 4.21 3 673 676 3 859.57 0.38 4 673 677 4 832.26 0.70 5 673 723 50 26.73 0.84 6 673 806 133 24.76 0.07 7 673 834 161 24.71 0.07 8 673 837 164 24.71 0.07 9 674 677 3 848.81 3.90 10 674 678 4 835.27 2.20 11 674 802 128 24.95 0.12 12 674 806 132 24.88 0.09 13 674 841 167 26.70 1.00 14 675 678 3 862.49 7.60 15 675 679 4 828.40 5.63 16 676 679 3 839.12 8.72 17 676 680 4 828.40 5.64 18 676 723 47 32.22 0.34 19 677 680 3 846.60 4.99 20 677 681 4 819.53 1.51 21 677 722 45 33.77 0.08 22 677 723 46 33.79 0.09 23 677 805 128 32.12 0.29 24 677 806 129 32.15 0.30 25 677 809 132 32.15 0.30 26 678 681 3 841.62 6.87 27 678 805 127 32.16 0.30 28 680 718 38 34.02 0.07 29 680 721 41 34.03 0.07 30 680 722 42 34.02 0.07 31 681 718 37 34.43 0.14 32 681 804 123 34.11 0.12 33 681 805 124 33.98 0.07 34 681 808 127 33.99 0.07 35 682 714 32 35.35 0.06 36 682 718 36 34.95 0.10 37 684 766 82 81.25 0.17 38 685 707 22 81.17 0.20 39 685 710 25 80.92 0.17 40 685 711 26 77.14 0.66 41 685 714 29 77.47 0.51 42 685 765 80 81.10 0.15 43 685 766 81 81.31 0.15 44 686 707 21 81.28 0.18 45 686 710 24 81.28 0.18 46 686 765 79 81.17 0.18 47 686 766 80 81.31 0.15 48 686 767 81 81.34 0.14 49 687 706 19 81.34 0.21 50 687 707 20 81.29 0.18 51 687 710 23 81.30 0.18 52 687 765 78 81.33 0.16 53 687 767 80 81.44 0.12 54 687 768 81 81.35 0.14 55 688 710 22 81.43 0.18 56 688 767 79 81.72 0.25 57 689 700 11 81.77 0.28 58 689 703 14 81.68 0.29 59 689 767 78 81.73 0.24 60 689 768 79 81.71 0.24 61 689 774 85 81.88 0.29 62 690 693 3 856.27 3.29 63 691 767 76 81.88 0.26 64 691 774 83 91.45 3.93 65 695 700 5 803.38 7.02 66 695 773 78 125.09 1.89 67 695 774 79 121.37 0.05 68 695 777 82 125.09 1.89 69 696 699 3 844.16 4.71 70 696 700 4 818.70 0.63 71 697 700 3 844.16 4.71 72 697 701 4 825.77 4.17 73 697 777 80 125.44 1.78 74 697 778 81 125.43 1.78 75 697 779 82 125.43 1.78 76 697 876 179 132.78 0.90 77 697 880 183 145.28 1.17 78 697 887 190 146.48 1.06 79 697 889 192 146.49 1.06 80 698 701 3 842.46 4.17 81 698 702 4 830.46 1.82 82 698 887 189 146.50 1.06 83 698 888 190 146.52 1.06 84 698 889 191 146.49 1.06 85 699 702 3 833.62 0.30 86 699 703 4 828.78 0.98 87 700 703 3 833.00 0.69 88 700 704 4 825.01 0.89 89 700 768 68 146.53 1.07 90 700 770 70 146.50 1.06 91 700 774 74 146.50 1.06 92 700 777 77 146.76 0.96 93 700 778 78 146.60 1.03 94 701 704 3 827.44 2.10 95 701 705 4 810.98 2.38 96 701 778 77 146.77 0.96 97 701 876 175 146.62 1.04 98 701 880 179 146.87 0.91 99 701 889 188 149.35 0.45 100 702 705 3 826.24 1.50 101 702 706 4 826.01 1.61 102 702 889 187 149.35 0.45 103 702 890 188 149.39 0.47 104 703 706 3 842.96 5.30 105 703 707 4 830.65 0.73 106 703 768 65 149.77 0.61 107 704 707 3 830.67 0.72 108 704 708 4 830.65 0.72 109 704 764 60 149.75 0.60 110 704 768 64 158.19 2.08 111 704 770 66 151.44 0.22 112 704 780 76 149.78 0.61 113 705 708 3 830.66 0.72 114 705 709 4 835.86 1.88 115 705 889 184 163.94 0.66 116 705 890 185 163.77 0.59 117 705 891 186 162.43 0.69 118 705 892 187 163.77 0.58 119 705 895 190 161.13 1.11 120 706 709 3 838.80 1.75 121 706 710 4 835.85 1.88 122 706 892 186 164.08 0.70 123 707 710 3 835.85 1.89 124 707 711 4 835.84 1.89 125 707 764 57 161.88 0.93 126 707 765 58 162.02 0.87 127 707 768 61 162.53 0.63 128 708 711 3 845.00 2.69 129 708 712 4 828.87 5.37 130 708 741 33 162.50 0.63 131 708 745 37 161.94 0.90 132 708 895 187 164.59 0.67 133 709 712 3 827.90 4.88 134 709 713 4 823.19 2.53 135 709 892 183 165.54 0.32 136 709 894 185 164.85 0.74 137 709 895 186 164.69 0.69 138 710 713 3 839.28 4.21 139 710 714 4 830.02 0.88 140 711 714 3 830.95 1.02 141 711 715 4 829.57 0.65 142 711 745 34 164.94 0.57 143 711 752 41 166.91 0.39 144 712 715 3 834.00 1.56 145 712 716 4 829.57 0.65 146 712 737 25 166.28 0.67 147 712 741 29 165.55 0.32 148 712 894 182 173.22 1.05 149 712 898 186 172.96 1.16 150 713 716 3 841.76 0.93 151 713 717 4 832.47 0.81 152 713 894 181 173.37 1.04 153 714 717 3 834.80 0.89 154 714 718 4 816.75 4.58 155 715 718 3 830.42 0.12 156 715 719 4 819.78 2.12 157 715 737 22 176.28 0.39 158 715 744 29 171.78 0.36 159 716 719 3 837.95 2.35 160 716 720 4 822.21 0.92 161 716 734 18 185.62 0.41 162 716 737 21 180.58 1.77 163 717 720 3 840.94 1.47 164 717 721 4 829.01 2.18 165 718 721 3 834.31 1.85 166 718 722 4 829.01 2.18 167 718 744 26 186.06 0.23 168 718 808 90 186.06 0.23 169 718 809 91 186.06 0.23 170 718 812 94 186.09 0.23 171 719 722 3 852.51 4.90 172 719 725 6 652.82 9.62 173 719 737 18 186.78 0.58 174 719 812 93 186.08 0.24 175 720 725 5 672.39 0.17 176 720 726 6 643.66 14.20 177 720 733 13 187.56 0.51 178 720 734 14 187.58 0.49 179 720 737 17 187.54 0.50 180 722 725 3 841.43 5.54 181 722 809 87 187.42 0.44 182 722 834 112 187.44 0.42 183 723 830 107 254.89 34.12 184 723 834 111 187.47 0.43 185 724 727 3 824.36 4.40 186 724 830 106 254.88 34.12 187 725 728 3 848.40 6.11 188 725 733 8 356.56 84.03 189 725 737 12 347.17 79.39 190 725 812 87 198.31 5.03 191 725 830 105 269.91 41.62 192 726 733 7 707.94 35.65 193 727 826 99 287.89 49.68 194 727 829 102 287.88 49.68 195 727 830 103 287.85 49.66 196 728 732 4 818.75 0.95 197 728 733 5 758.90 10.17 198 728 813 85 287.92 49.62 199 728 826 98 288.33 49.87 200 728 827 99 287.89 49.68 201 728 830 102 287.88 49.67 202 729 732 3 839.87 4.26 203 729 733 4 829.84 0.24 204 729 822 93 303.45 45.69 205 729 823 94 302.33 45.88 206 730 733 3 837.28 0.48 207 731 734 3 854.41 1.58 208 731 735 4 832.45 1.04 209 731 822 91 320.54 42.60 210 732 735 3 840.12 0.72 211 732 736 4 832.41 1.04 212 732 821 89 320.50 42.59 213 732 822 90 320.55 42.60 214 732 823 91 320.60 42.63 215 733 736 3 850.68 3.46 216 733 737 4 833.06 1.36 217 734 737 3 835.41 0.45 218 734 738 4 820.48 0.04 219 735 738 3 827.70 0.83 220 735 739 4 820.47 0.04 221 736 739 3 837.87 2.54 222 736 740 4 820.46 0.03 223 736 812 76 323.87 41.01 224 736 813 77 324.56 41.39 225 736 821 85 334.44 40.69 226 736 827 91 328.98 43.55 227 737 740 3 827.77 0.26 228 737 741 4 814.63 2.85 229 737 812 75 328.49 42.04 230 738 741 3 822.96 3.21 231 738 898 160 276.30 0.12 232 738 902 164 263.61 3.85 233 739 815 76 335.59 40.24 234 739 817 78 335.76 40.15 235 739 902 163 273.13 1.44 236 739 905 166 263.58 3.84 237 739 906 167 257.38 0.97 238 739 911 172 257.25 0.92 239 740 743 3 842.97 2.67 240 740 744 4 826.01 1.71 241 740 811 71 335.70 40.21 242 740 812 72 335.73 40.20 243 740 815 75 335.71 40.19 244 741 744 3 838.48 4.92 245 741 745 4 826.02 1.71 246 741 895 154 291.02 2.21 247 741 898 157 289.48 2.73 248 742 745 3 841.38 3.46 249 742 746 4 832.92 1.21 250 742 870 128 346.87 34.41 251 742 873 131 339.37 30.55 252 742 874 132 326.18 24.00 253 742 877 135 314.88 14.46 254 743 746 3 832.91 1.22 255 743 747 4 834.90 0.21 256 743 811 68 349.22 35.47 257 743 866 123 346.95 34.54 258 743 870 127 347.09 34.46 259 744 747 3 845.32 0.80 260 744 748 4 834.91 0.22 261 744 808 64 349.47 35.58 262 744 811 67 349.22 35.46 263 744 812 68 349.23 35.46 264 745 748 3 856.45 5.01 265 745 749 4 823.50 5.50 266 746 749 3 834.86 0.15 267 746 750 4 834.87 0.18 268 746 787 41 354.24 33.20 269 746 790 44 354.26 33.20 270 746 866 120 353.72 33.45 271 746 869 123 353.42 33.30 272 746 870 124 353.35 33.27 273 746 873 127 353.25 33.20 274 747 750 3 846.07 5.78 275 747 751 4 834.88 0.18 276 747 790 43 364.58 38.36 277 747 794 47 364.94 38.40 278 747 804 57 365.00 38.43 279 747 807 60 364.91 38.40 280 747 808 61 364.81 38.38 281 747 811 64 364.81 38.38 282 747 863 116 354.21 33.28 283 747 866 119 353.83 33.40 284 748 751 3 850.35 3.64 285 748 752 4 834.65 0.29 286 748 804 56 365.13 38.44 287 748 808 60 364.96 38.41 288 749 752 3 843.25 4.00 289 749 753 4 839.15 1.96 290 749 762 13 370.05 38.32 291 749 763 14 370.48 38.55 292 749 764 15 369.88 38.37 293 749 787 38 367.36 38.58 294 750 753 3 839.12 1.98 295 750 754 4 839.09 1.99 296 750 791 41 367.25 38.49 297 750 794 44 367.28 38.48 298 751 754 3 840.48 2.19 299 751 755 4 836.46 4.21 300 751 794 43 367.31 38.45 301 751 799 48 367.26 38.47 302 751 800 49 367.28 38.47 303 751 801 50 367.22 38.45 304 751 804 53 366.22 37.99 305 752 755 3 850.53 4.24 306 752 756 4 808.57 3.42 307 752 763 11 370.67 38.62 308 752 764 12 370.74 38.59 309 753 756 3 846.40 1.76 310 753 757 4 799.78 7.36 311 753 758 5 725.98 0.06 312 753 759 6 414.06 60.15 313 753 761 8 402.63 54.43 314 753 762 9 386.17 46.36 315 753 763 10 370.66 38.63 316 753 791 38 367.54 38.55 317 754 758 4 662.74 4.22 318 754 759 5 553.90 4.47 319 754 791 37 367.54 38.55 320 754 794 40 367.39 38.48 321 754 795 41 367.39 38.48 322 756 763 7 390.38 48.30 323 757 760 3 847.62 1.17 324 757 761 4 796.38 10.04 325 757 763 6 414.04 60.12 326 759 788 29 367.64 38.53 327 759 791 32 367.62 38.52 328 759 792 33 367.61 38.52 329 760 788 28 367.87 38.43 330 761 769 8 371.47 38.94 331 761 770 9 371.11 39.09 332 761 771 10 370.97 39.14 333 761 775 14 370.97 39.15 334 762 769 7 371.47 38.94 335 762 770 8 371.43 38.94 336 762 775 13 370.98 39.16 337 762 784 22 367.85 38.48 338 762 787 25 367.66 38.53 339 762 788 26 367.62 38.52 340 763 766 3 896.88 0.90 341 763 769 6 371.47 38.93 342 764 768 4 800.84 9.74 343 764 770 6 634.54 7.36 344 764 780 16 370.26 39.52 345 765 768 3 845.50 0.23 346 765 769 4 808.15 6.08 347 766 769 3 884.74 6.25 348 768 774 6 803.27 6.22 349 769 774 5 806.37 4.71 350 770 774 4 803.27 6.22 351 770 775 5 803.26 6.22 352 770 778 8 790.05 9.13 353 770 784 14 369.61 37.62 354 771 774 3 845.42 4.94 355 771 775 4 828.20 3.67 356 772 775 3 834.95 0.30 357 772 776 4 828.21 3.67 358 772 781 9 618.50 37.62 359 773 776 3 853.66 1.91 360 773 777 4 824.59 5.47 361 774 777 3 842.69 3.58 362 774 778 4 824.58 5.46 363 775 778 3 853.51 1.82 364 775 780 5 673.82 9.97 365 775 781 6 534.40 1.38 366 775 784 9 391.50 48.56 367 775 788 13 372.27 38.94 368 776 779 3 853.51 1.82 369 776 781 5 629.81 31.97 370 778 876 98 418.30 11.05 371 779 876 97 418.35 11.03 372 780 783 3 856.18 0.51 373 780 784 4 816.83 1.75 374 780 873 93 418.59 11.17 375 780 876 96 418.30 11.01 376 781 784 3 832.73 1.10 377 781 785 4 827.65 3.64 378 782 785 3 847.11 3.56 379 782 786 4 846.27 4.87 380 783 786 3 846.27 4.87 381 783 787 4 846.32 4.84 382 783 872 89 418.97 11.00 383 783 873 90 418.95 10.98 384 783 876 93 418.69 10.85 385 784 787 3 846.33 4.84 386 784 788 4 846.32 4.84 387 785 788 3 846.34 4.84 388 785 789 4 846.32 4.84 389 786 789 3 839.95 1.65 390 786 790 4 826.62 4.98 391 786 868 82 419.48 11.21 392 786 869 83 419.45 11.20 393 786 872 86 419.44 11.19 394 787 790 3 826.83 5.08 395 787 791 4 813.86 3.61 396 787 869 82 419.45 11.18 397 787 873 86 419.01 10.96 398 788 791 3 829.88 3.54 399 788 792 4 813.77 3.64 400 789 792 3 839.93 1.48 401 789 793 4 814.93 3.99 402 789 796 7 799.13 3.56 403 790 793 3 826.12 8.62 404 790 794 4 834.57 4.11 405 790 862 72 419.67 11.30 406 790 869 79 419.87 10.98 407 791 794 3 849.61 2.16 408 792 795 3 858.93 6.88 409 792 796 4 832.93 4.93 410 793 796 3 834.57 4.11 411 793 797 4 814.90 3.99 412 793 862 69 420.38 11.23 413 794 798 4 787.98 1.94 414 794 799 5 626.57 19.23 415 794 804 10 528.06 5.67 416 794 862 68 420.40 11.22 417 795 798 3 828.13 11.08 418 797 855 58 480.20 7.13 419 797 858 61 471.91 3.14 420 797 862 65 421.51 11.70 421 798 855 57 486.29 9.55 422 799 803 4 830.69 12.61 423 799 804 5 809.41 3.75 424 799 807 8 792.74 1.29 425 799 855 56 490.28 11.54 426 799 859 60 478.61 6.43 427 799 862 63 474.20 4.25 428 800 803 3 830.71 12.60 429 800 804 4 845.65 5.11 430 801 804 3 845.72 5.09 431 801 805 4 845.70 5.09 432 802 805 3 847.01 4.45 433 802 806 4 845.69 5.09 434 802 841 39 491.94 11.59 435 803 806 3 855.67 0.10 436 803 807 4 832.86 1.32 437 803 841 38 491.94 11.58 438 803 842 39 491.93 11.58 439 803 855 52 491.12 11.51 440 803 859 56 490.97 11.51 441 804 807 3 843.83 2.34 442 804 808 4 832.72 1.39 443 805 808 3 843.03 1.94 444 805 809 4 840.82 2.30 445 806 809 3 852.15 3.03 446 806 810 4 840.99 2.36 447 806 838 32 492.11 11.56 448 806 841 35 492.10 11.56 449 807 810 3 846.43 0.26 450 807 811 4 843.63 1.66 451 807 838 31 492.12 11.58 452 807 859 52 491.04 11.51 453 807 862 55 491.03 11.50 454 807 863 56 490.49 11.26 455 808 811 3 846.54 0.23 456 808 812 4 846.53 0.23 457 809 812 3 847.36 0.59 458 809 813 4 846.53 0.23 459 809 834 25 492.44 11.63 460 810 813 3 853.60 3.75 461 810 814 4 846.53 0.23 462 810 831 21 492.51 11.60 463 810 834 24 492.44 11.63 464 810 835 25 492.44 11.63 465 810 838 28 492.33 11.58 466 810 863 53 491.05 11.51 467 810 916 106 381.25 43.59 468 811 814 3 845.78 0.13 469 811 863 52 490.92 11.45 470 811 866 55 490.77 11.40 471 811 867 56 490.59 11.46 472 813 827 14 492.98 11.51 473 813 830 17 492.98 11.51 474 813 831 18 492.94 11.52 475 813 834 21 492.92 11.51 476 813 914 101 381.94 43.48 477 814 831 17 492.97 11.52 478 814 867 53 490.75 11.46 479 814 913 99 381.95 43.48 480 814 914 100 381.93 43.48 481 815 906 91 384.99 43.12 482 815 911 96 384.11 43.41 483 815 912 97 383.05 43.39 484 815 913 98 381.95 43.48 485 815 914 99 381.95 43.48 486 816 821 5 636.83 63.69 487 816 824 8 494.75 11.17 488 816 827 11 493.31 11.50 489 816 911 95 384.39 43.30 490 816 912 96 384.03 43.38 491 816 914 98 382.94 43.27 492 817 820 3 821.33 0.84 493 817 821 4 791.91 13.86 494 817 910 93 384.69 43.23 495 817 911 94 384.50 43.25 496 817 912 95 384.50 43.25 497 818 910 92 384.73 43.24 498 818 911 93 384.69 43.25 499 818 912 94 384.63 43.23 500 820 824 4 745.47 5.49 501 821 824 3 812.52 4.82 502 821 827 6 782.23 11.85 503 823 826 3 855.45 2.21 504 823 827 4 852.73 3.57 505 824 827 3 852.74 3.57 506 824 828 4 841.63 9.12 507 825 828 3 858.35 0.76 508 825 829 4 845.37 7.25 509 826 829 3 858.34 0.75 510 826 830 4 819.84 3.90 511 827 830 3 845.36 7.24 512 827 831 4 819.83 3.89 513 827 914 87 382.92 43.27 514 828 831 3 846.64 0.65 515 828 832 4 824.99 6.42 516 829 832 3 846.65 0.65 517 829 833 4 841.58 1.88 518 830 833 3 845.88 0.26 519 830 834 4 845.78 0.21 520 831 834 3 847.97 1.31 521 831 835 4 829.61 8.31 522 831 914 83 382.38 43.26 523 831 916 85 382.15 43.37 524 832 835 3 842.76 1.74 525 832 836 4 834.45 5.90 526 833 836 3 846.32 1.41 527 833 837 4 839.77 3.23 528 834 837 3 839.78 3.23 529 834 838 4 839.78 3.23 530 835 838 3 837.21 4.56 531 835 839 4 830.74 1.33 532 835 856 21 491.39 11.53 533 835 916 81 381.43 43.41 534 836 839 3 839.64 4.11 535 836 840 4 830.74 1.34 536 837 840 3 839.85 3.21 537 837 841 4 826.91 3.26 538 838 841 3 844.15 1.86 539 838 842 4 830.75 1.33 540 838 863 25 491.11 11.54 541 839 842 3 839.89 3.23 542 839 843 4 815.42 1.33 543 840 843 3 841.20 3.88 544 840 844 4 822.96 2.46 545 841 844 3 860.95 4.78 546 842 852 10 491.21 11.50 547 842 855 13 491.12 11.51 548 842 856 14 491.11 11.52 549 842 859 17 491.09 11.53 550 847 852 5 519.41 2.86 551 848 851 3 855.58 1.48 552 848 852 4 663.38 15.13 553 849 852 3 867.14 7.25 554 849 853 4 815.40 3.92 555 850 854 4 796.18 13.53 556 851 854 3 825.14 0.94 557 851 855 4 813.26 4.99 558 852 855 3 841.37 3.39 559 852 856 4 825.15 0.94 560 853 856 3 830.82 1.90 561 853 857 4 818.47 2.40 562 854 857 3 852.15 2.98 563 854 858 4 812.13 3.16 564 855 858 3 839.63 6.38 565 855 859 4 820.63 0.70 566 856 859 3 852.58 0.09 567 856 860 4 827.81 5.71 568 857 860 3 852.58 0.09 569 857 861 4 834.47 2.39 570 857 917 60 380.76 43.46 571 858 861 3 835.12 2.52 572 858 862 4 834.46 2.39 573 859 862 3 834.46 2.38 574 859 863 4 837.87 0.67 575 860 863 3 845.58 3.18 576 860 864 4 837.87 0.67 577 860 916 56 380.62 43.55 578 860 917 57 380.62 43.55 579 861 864 3 837.88 0.67 580 861 865 4 840.83 0.82 581 861 917 56 380.60 43.55 582 861 918 57 380.62 43.56 583 862 865 3 841.38 0.63 584 862 866 4 847.83 2.60 585 863 866 3 860.69 1.09 586 863 916 53 380.88 43.68 587 864 867 3 861.58 1.54 588 864 868 4 855.72 1.39 589 864 915 51 380.88 43.68 590 864 916 52 380.88 43.68 591 864 917 53 380.86 43.69 592 865 868 3 868.26 0.43 593 865 869 4 855.29 1.18 594 866 869 3 855.29 1.18 595 866 870 4 839.33 1.03 596 867 870 3 843.51 0.89 597 867 871 4 839.30 1.05 598 867 913 46 381.43 43.74 599 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