BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 10 145 135 88.60 0.30 2 11 14 3 757.18 4.54 3 11 15 4 728.29 1.11 4 11 74 63 81.03 1.41 5 11 167 156 89.89 2.54 6 12 15 3 740.17 0.58 7 12 16 4 729.61 0.45 8 13 16 3 744.75 1.48 9 13 17 4 741.20 3.25 10 13 167 154 98.11 0.65 11 14 17 3 744.73 1.49 12 14 18 4 734.46 0.52 13 14 74 60 98.12 0.65 14 14 75 61 98.12 0.65 15 14 76 62 98.18 0.67 16 14 167 153 98.12 0.65 17 14 168 154 98.11 0.65 18 15 18 3 746.00 1.64 19 15 76 61 99.17 1.17 20 16 19 3 760.50 2.57 21 17 20 3 743.83 0.55 22 17 37 20 322.45 5.66 23 17 168 151 148.01 21.96 24 18 36 18 333.95 1.73 25 18 37 19 322.31 5.73 26 18 38 20 307.84 3.03 27 18 76 58 207.47 45.78 28 18 145 127 311.31 1.28 29 19 36 17 337.00 0.58 30 20 36 16 339.57 0.10 31 20 37 17 338.81 0.12 32 20 91 71 320.32 1.45 33 21 34 13 339.99 0.06 34 21 35 14 339.91 0.04 35 21 36 15 339.88 0.04 36 21 91 70 331.64 2.06 37 21 92 71 333.70 1.59 38 22 34 12 340.03 0.06 39 22 35 13 340.00 0.05 40 22 37 15 339.93 0.04 41 22 40 18 339.86 0.05 42 22 52 30 336.79 0.39 43 22 89 67 332.88 1.43 44 22 92 70 334.06 1.70 45 23 31 8 428.30 44.01 46 23 33 10 427.89 43.94 47 23 52 29 337.99 0.28 48 23 53 30 334.21 1.64 49 23 90 67 335.97 0.76 50 23 91 68 336.01 0.74 51 23 92 69 335.99 0.75 52 23 96 73 335.98 0.76 53 23 97 74 334.22 1.64 54 24 31 7 430.60 45.12 55 24 32 8 428.24 43.98 56 24 33 9 427.89 43.94 57 24 34 10 340.18 0.09 58 24 35 11 340.18 0.09 59 24 40 16 340.01 0.06 60 24 50 26 340.01 0.06 61 24 51 27 339.86 0.04 62 24 52 28 338.75 0.10 63 25 29 4 615.07 4.54 64 25 30 5 563.23 4.43 65 25 31 6 430.61 45.12 66 25 32 7 430.59 45.12 67 25 51 26 340.02 0.07 68 25 52 27 339.88 0.03 69 25 53 28 339.81 0.03 70 25 150 125 339.86 0.04 71 26 29 3 730.80 2.45 72 26 30 4 635.22 14.62 73 26 32 6 546.14 12.63 74 26 51 25 340.08 0.07 75 26 150 124 340.06 0.08 76 27 51 24 340.14 0.09 77 27 136 109 340.01 0.10 78 27 150 123 340.06 0.08 79 27 225 198 44.87 0.20 80 27 227 200 44.51 0.21 81 28 225 197 44.87 0.20 82 28 227 199 44.76 0.14 83 29 53 24 340.01 0.09 84 29 134 105 339.97 0.09 85 29 150 121 340.00 0.10 86 29 152 123 340.01 0.09 87 29 225 196 44.86 0.20 88 31 53 22 339.92 0.04 89 31 97 66 339.73 0.07 90 34 92 58 334.19 1.64 91 35 39 4 733.33 6.02 92 35 40 5 717.49 8.90 93 35 50 15 352.95 0.78 94 36 39 3 746.72 0.68 95 36 40 4 745.03 1.48 96 37 40 3 746.69 0.66 97 37 41 4 745.02 1.48 98 37 89 52 341.37 0.34 99 38 41 3 746.69 0.66 100 38 42 4 745.02 1.48 101 38 145 107 374.53 0.07 102 39 42 3 745.11 1.51 103 39 45 6 693.47 0.53 104 39 50 11 554.27 8.31 105 40 46 6 710.58 6.58 106 40 50 10 447.78 36.89 107 40 52 12 372.47 0.79 108 41 46 5 728.16 1.86 109 41 52 11 447.78 36.88 110 41 77 36 374.41 0.01 111 41 89 48 374.16 0.11 112 41 145 104 379.98 0.86 113 41 146 105 375.28 0.16 114 41 147 106 374.22 0.10 115 42 45 3 745.82 0.22 116 42 46 4 729.58 1.89 117 42 144 102 380.66 0.92 118 42 145 103 380.24 0.72 119 43 46 3 732.95 3.33 120 43 47 4 729.48 1.73 121 43 140 97 380.62 0.88 122 43 142 99 380.94 1.03 123 43 143 100 380.95 1.03 124 43 144 101 380.86 0.99 125 43 145 102 380.66 0.92 126 43 146 103 380.28 0.72 127 44 47 3 743.46 0.96 128 44 48 4 737.76 1.90 129 45 48 3 743.84 0.33 130 45 49 4 741.92 0.19 131 45 50 5 693.06 0.61 132 46 49 3 729.64 1.79 133 46 50 4 710.88 6.71 134 46 138 92 381.14 0.99 135 46 146 100 381.16 0.98 136 46 147 101 381.17 0.97 137 46 148 102 381.07 0.96 138 50 148 98 498.51 0.25 139 51 136 85 498.53 0.25 140 51 148 97 498.56 0.23 141 51 149 98 498.55 0.23 142 51 150 99 498.53 0.25 143 52 89 37 532.15 16.44 144 52 147 95 498.62 0.23 145 52 148 96 498.59 0.23 146 52 149 97 498.57 0.24 147 52 150 98 498.56 0.23 148 53 89 36 532.15 16.44 149 53 90 37 532.14 16.44 150 53 99 46 531.45 16.19 151 53 105 52 531.31 16.12 152 53 150 97 498.57 0.24 153 53 152 99 498.55 0.24 154 54 88 34 532.22 16.48 155 54 89 35 532.15 16.44 156 54 90 36 532.15 16.44 157 54 149 95 498.62 0.24 158 54 150 96 498.60 0.24 159 54 151 97 498.56 0.24 160 54 152 98 498.56 0.24 161 55 86 31 532.42 16.42 162 55 87 32 532.40 16.41 163 55 88 33 532.32 16.44 164 55 89 34 532.28 16.43 165 55 90 35 532.24 16.45 166 55 105 50 531.41 16.16 167 55 108 53 531.44 16.14 168 55 109 54 531.31 16.12 169 55 112 57 531.50 16.04 170 55 151 96 498.59 0.23 171 55 152 97 498.56 0.24 172 55 154 99 498.55 0.24 173 56 84 28 532.58 16.55 174 56 86 30 532.44 16.41 175 56 87 31 532.40 16.41 176 56 151 95 498.63 0.24 177 56 152 96 498.61 0.24 178 56 153 97 498.57 0.23 179 56 154 98 498.57 0.24 180 56 197 141 171.09 0.12 181 56 201 145 165.34 0.45 182 57 84 27 532.67 16.52 183 57 85 28 532.65 16.51 184 57 86 29 532.45 16.41 185 57 109 52 531.57 16.07 186 57 113 56 531.55 16.04 187 57 154 97 498.57 0.23 188 57 156 99 498.56 0.23 189 58 66 8 532.94 16.61 190 58 84 26 532.67 16.52 191 58 85 27 532.67 16.52 192 58 153 95 498.64 0.23 193 58 154 96 498.60 0.23 194 58 155 97 498.57 0.23 195 58 156 98 498.57 0.24 196 58 198 140 171.34 0.23 197 58 201 143 167.29 0.18 198 58 206 148 149.54 0.47 199 59 66 7 532.95 16.61 200 59 67 8 532.90 16.62 201 59 85 26 532.70 16.52 202 59 119 60 515.00 7.73 203 59 156 97 498.57 0.23 204 59 158 99 498.55 0.24 205 59 160 101 428.80 1.43 206 59 174 115 271.68 0.36 207 60 65 5 553.73 6.32 208 60 66 6 532.96 16.61 209 60 155 95 498.63 0.24 210 60 156 96 498.59 0.24 211 60 157 97 498.56 0.23 212 60 158 98 498.56 0.23 213 60 206 146 156.41 2.94 214 60 210 150 145.75 0.98 215 60 213 153 116.04 2.34 216 61 64 3 735.05 0.75 217 61 65 4 602.81 5.21 218 61 158 97 498.56 0.24 219 61 159 98 498.54 0.23 220 61 174 113 271.77 0.35 221 62 159 97 498.57 0.23 222 64 210 146 149.04 0.27 223 64 213 149 140.41 0.79 224 65 174 109 271.79 0.35 225 65 175 110 271.56 0.36 226 65 176 111 271.08 0.40 227 65 179 114 245.93 0.33 228 65 210 145 149.07 0.27 229 66 85 19 534.94 16.98 230 66 173 107 272.51 0.43 231 66 174 108 271.56 0.36 232 66 175 109 271.32 0.38 233 66 198 132 172.09 0.20 234 66 206 140 163.30 0.51 235 66 210 144 145.78 0.98 236 67 85 18 534.94 16.98 237 67 117 50 531.94 15.41 238 67 162 95 398.23 0.12 239 67 172 105 272.51 0.42 240 67 173 106 271.95 0.26 241 67 174 107 271.35 0.38 242 68 83 15 535.21 16.91 243 68 84 16 535.17 16.92 244 68 172 104 272.33 0.35 245 68 173 105 271.95 0.26 246 68 175 107 271.49 0.39 247 68 180 112 214.84 1.07 248 68 183 115 175.66 1.05 249 68 198 130 172.08 0.20 250 69 82 13 538.13 15.55 251 69 83 14 535.26 16.91 252 69 84 15 535.25 16.91 253 69 85 16 535.13 16.95 254 69 86 17 535.13 16.95 255 69 116 47 531.98 15.38 256 69 169 100 285.57 1.93 257 69 171 102 281.33 2.17 258 69 172 103 271.99 0.29 259 70 80 10 539.52 15.20 260 70 81 11 539.29 15.26 261 70 82 12 538.12 15.56 262 70 169 99 285.57 1.93 263 70 170 100 285.54 1.92 264 70 171 101 284.97 1.90 265 71 75 4 732.47 1.30 266 71 77 6 623.88 22.20 267 71 78 7 539.74 15.14 268 71 79 8 539.60 15.20 269 71 80 9 539.53 15.20 270 71 81 10 539.51 15.20 271 71 83 12 539.27 15.25 272 71 168 97 285.70 1.92 273 71 169 98 285.56 1.94 274 71 170 99 285.56 1.94 275 71 195 124 172.22 0.24 276 71 197 126 172.23 0.23 277 72 75 3 742.07 0.22 278 72 79 7 539.62 15.20 279 72 80 8 539.56 15.20 280 72 166 94 285.82 1.92 281 72 167 95 285.80 1.92 282 72 168 96 285.69 1.91 283 72 169 97 285.56 1.94 284 72 170 98 285.51 1.96 285 73 79 6 562.06 3.98 286 73 80 7 539.62 15.20 287 73 170 97 285.58 1.94 288 74 167 93 285.72 1.91 289 75 79 4 575.11 1.21 290 75 168 93 285.58 1.95 291 76 145 69 499.44 0.30 292 77 141 64 499.45 0.30 293 77 145 68 499.44 0.29 294 77 146 69 499.36 0.29 295 78 81 3 749.21 3.41 296 78 139 61 499.47 0.33 297 78 140 62 499.47 0.32 298 78 141 63 499.44 0.31 299 78 147 69 499.39 0.30 300 78 195 117 171.55 0.30 301 78 196 118 171.96 0.17 302 78 197 119 172.01 0.20 303 79 193 114 172.27 0.24 304 80 170 90 285.56 1.94 305 81 193 112 172.27 0.24 306 81 194 113 172.19 0.26 307 81 195 114 172.20 0.24 308 82 183 101 172.31 0.27 309 82 192 110 172.28 0.24 310 82 193 111 172.27 0.24 311 82 194 112 172.19 0.26 312 82 195 113 172.22 0.24 313 83 195 112 172.02 0.20 314 83 197 114 172.06 0.21 315 83 198 115 171.12 0.12 316 84 87 3 815.16 0.72 317 84 197 113 172.03 0.21 318 84 198 114 171.09 0.12 319 85 113 28 533.50 15.11 320 85 117 32 520.60 8.52 321 86 112 26 533.68 15.03 322 86 113 27 533.38 15.05 323 86 116 30 525.26 10.81 324 87 197 110 171.06 0.14 325 88 112 24 533.58 14.96 326 89 111 22 533.83 14.88 327 89 147 58 498.72 0.22 328 89 149 60 498.69 0.21 329 90 96 6 571.29 0.24 330 90 105 15 533.59 14.97 331 90 108 18 533.63 14.95 332 91 96 5 633.13 13.63 333 91 111 20 533.96 14.87 334 92 97 5 651.85 14.83 335 93 96 3 753.73 1.50 336 93 97 4 731.58 1.22 337 94 97 3 744.91 0.85 338 94 98 4 736.72 1.34 339 95 98 3 762.31 0.73 340 95 104 9 550.35 6.92 341 95 107 12 550.21 6.88 342 96 104 8 550.34 6.93 343 96 107 11 550.19 6.88 344 96 108 12 550.18 6.87 345 96 111 15 550.19 6.88 346 98 103 5 574.33 1.54 347 98 104 6 550.45 6.91 348 98 107 9 553.94 8.65 349 99 102 3 723.11 9.17 350 100 103 3 743.94 1.25 351 101 106 5 739.58 0.37 352 102 105 3 751.84 2.98 353 102 106 4 739.58 0.38 354 102 131 29 501.48 1.17 355 102 152 50 498.72 0.24 356 103 106 3 751.93 0.88 357 103 107 4 739.58 0.38 358 103 126 23 504.55 0.25 359 104 107 3 744.05 2.39 360 104 108 4 739.58 0.38 361 105 108 3 739.57 0.38 362 105 109 4 746.90 4.66 363 105 152 47 498.68 0.23 364 105 154 49 498.62 0.21 365 106 109 3 746.90 4.66 366 106 110 4 746.90 4.67 367 106 126 20 504.56 0.25 368 106 127 21 504.56 0.25 369 106 129 23 502.42 1.02 370 106 154 48 498.63 0.21 371 107 110 3 746.72 4.76 372 107 111 4 746.71 4.76 373 107 126 19 504.56 0.25 374 108 111 3 746.70 4.77 375 108 112 4 746.70 4.77 376 109 112 3 746.71 4.78 377 109 113 4 744.19 6.03 378 109 129 20 502.65 1.14 379 109 154 45 498.61 0.23 380 110 113 3 746.68 4.79 381 110 114 4 734.33 1.38 382 110 121 11 504.76 0.23 383 110 124 14 504.59 0.24 384 110 125 15 504.59 0.24 385 110 126 16 504.57 0.25 386 110 129 19 502.68 1.09 387 111 114 3 741.26 0.87 388 111 115 4 726.99 2.29 389 111 121 10 513.96 4.83 390 112 115 3 729.52 1.02 391 112 116 4 726.95 2.31 392 112 168 56 285.19 1.81 393 113 117 4 671.41 1.00 394 113 118 5 658.59 0.84 395 113 119 6 578.37 3.98 396 113 124 11 504.63 0.26 397 113 129 16 504.50 0.29 398 114 118 4 608.08 9.36 399 114 119 5 585.86 0.24 400 114 121 7 545.36 20.50 401 115 165 50 285.88 1.89 402 116 164 48 285.92 1.89 403 116 165 49 285.88 1.89 404 116 168 52 285.19 1.81 405 117 162 45 286.06 1.88 406 117 163 46 286.05 1.86 407 117 164 47 285.93 1.89 408 117 169 52 285.40 1.86 409 118 163 45 286.00 1.88 410 118 164 46 285.94 1.90 411 119 123 4 746.75 4.48 412 119 124 5 725.84 0.58 413 119 160 41 423.37 3.23 414 119 161 42 412.78 2.36 415 119 162 43 286.15 1.81 416 120 123 3 761.50 3.62 417 120 124 4 746.75 4.48 418 120 161 41 424.38 2.71 419 121 124 3 761.51 3.62 420 121 125 4 746.74 4.48 421 122 125 3 769.22 0.23 422 123 128 5 725.79 0.58 423 123 158 35 498.41 0.19 424 123 160 37 432.31 0.86 425 123 161 38 431.93 1.05 426 124 128 4 708.90 9.03 427 124 129 5 708.88 9.04 428 124 156 32 498.57 0.23 429 124 160 36 432.31 0.86 430 125 128 3 742.85 3.88 431 125 129 4 732.46 0.49 432 126 129 3 732.47 0.49 433 128 156 28 498.62 0.23 434 128 158 30 498.56 0.23 435 129 154 25 498.68 0.21 436 129 155 26 498.64 0.22 437 129 156 27 498.57 0.24 438 130 154 24 498.69 0.23 439 130 155 25 498.66 0.21 440 130 216 86 69.34 0.35 441 131 152 21 498.78 0.23 442 131 153 22 498.73 0.22 443 131 154 23 498.68 0.24 444 131 222 91 48.84 0.58 445 132 153 21 498.77 0.23 446 132 155 23 498.70 0.23 447 132 209 77 142.62 0.32 448 132 216 84 64.93 1.22 449 132 220 88 49.30 0.80 450 132 221 89 49.30 0.80 451 132 222 90 48.88 0.58 452 133 152 19 498.82 0.23 453 133 153 20 498.78 0.23 454 133 205 72 149.54 0.48 455 133 209 76 142.63 0.32 456 133 221 88 48.88 0.58 457 133 224 91 48.29 0.58 458 133 226 93 44.59 0.24 459 134 150 16 499.12 0.21 460 134 151 17 498.86 0.23 461 134 152 18 498.83 0.23 462 134 222 88 48.85 0.57 463 134 223 89 48.71 0.64 464 134 225 91 44.51 0.23 465 134 226 92 44.48 0.24 466 135 150 15 499.13 0.20 467 135 151 16 499.03 0.21 468 135 153 18 498.88 0.21 469 135 200 65 166.81 0.42 470 135 201 66 165.33 0.43 471 135 205 70 149.55 0.49 472 135 226 91 44.29 0.29 473 135 228 93 39.22 0.55 474 136 148 12 499.43 0.29 475 136 149 13 499.34 0.28 476 136 150 14 499.13 0.20 477 136 225 89 44.51 0.23 478 136 226 90 44.38 0.24 479 136 227 91 38.73 0.80 480 136 228 92 29.14 0.59 481 137 147 10 499.54 0.31 482 137 148 11 499.44 0.29 483 137 149 12 499.42 0.30 484 137 151 14 499.34 0.29 485 137 196 59 171.19 0.20 486 137 197 60 171.12 0.17 487 137 200 63 165.35 0.45 488 137 228 91 24.48 1.44 489 138 147 9 499.53 0.30 490 138 148 10 499.45 0.29 491 139 146 7 499.68 0.29 492 139 147 8 499.66 0.29 493 139 196 57 171.18 0.20 494 140 145 5 521.62 11.26 495 140 146 6 499.68 0.29 496 141 145 4 615.37 1.70 497 141 146 5 521.53 11.22 498 142 145 3 743.75 1.36 499 147 197 50 171.06 0.14 500 150 225 75 44.55 0.24 501 153 201 48 165.31 0.46 502 153 209 56 142.63 0.32 503 155 209 54 142.76 0.38 504 155 213 58 99.98 1.76 505 155 216 61 69.30 0.32 506 157 213 56 107.19 1.27 507 157 214 57 107.16 1.26 508 160 174 14 271.89 0.32 509 162 172 10 283.41 1.29 510 164 169 5 642.04 5.81 511 164 172 8 412.39 66.09 512 165 168 3 745.14 0.39 513 165 169 4 733.29 6.32 514 166 169 3 734.15 5.88 515 169 172 3 829.59 1.47 516 175 179 4 727.35 0.53 517 175 180 5 713.03 2.16 518 175 210 35 149.63 0.43 519 176 179 3 736.05 4.88 520 176 180 4 736.03 4.88 521 176 210 34 149.64 0.43 522 177 180 3 738.03 3.90 523 177 181 4 739.44 3.19 524 177 207 30 165.10 0.53 525 177 210 33 149.64 0.43 526 177 211 34 149.63 0.43 527 178 181 3 739.44 3.19 528 178 182 4 739.44 3.19 529 179 182 3 746.08 0.14 530 179 183 4 730.31 0.94 531 180 183 3 736.87 4.24 532 180 184 4 729.40 1.39 533 180 198 18 172.28 0.24 534 180 206 26 165.58 0.58 535 180 207 27 165.22 0.49 536 180 210 30 149.68 0.43 537 181 184 3 741.50 4.57 538 181 185 4 723.97 1.33 539 181 207 26 165.31 0.46 540 182 185 3 748.19 1.24 541 182 186 4 730.18 0.91 542 183 186 3 741.34 4.67 543 183 187 4 741.38 4.65 544 183 192 9 368.30 98.09 545 184 187 3 747.53 1.56 546 184 188 4 737.04 4.34 547 184 194 10 172.41 0.24 548 184 202 18 171.65 0.04 549 184 207 23 165.31 0.46 550 185 188 3 747.34 0.81 551 185 189 4 743.97 0.88 552 186 189 3 751.00 2.64 553 186 190 4 743.97 0.88 554 186 191 5 669.16 7.68 555 186 192 6 368.32 98.10 556 187 190 3 740.46 3.20 557 187 192 5 368.28 98.10 558 187 194 7 368.21 98.09 559 194 198 4 723.10 4.16 560 194 199 5 713.78 0.45 561 195 198 3 746.44 1.93 562 195 199 4 719.73 2.48 563 196 199 3 749.23 1.28 564 196 200 4 731.24 1.60 565 197 200 3 749.23 1.28 566 197 201 4 724.62 4.92 567 198 201 3 746.38 2.69 568 198 202 4 724.59 4.92 569 198 206 8 662.18 6.80 570 199 202 3 748.51 1.63 571 199 203 4 738.26 6.75 572 200 203 3 743.04 4.36 573 200 204 4 738.25 6.75 574 200 228 28 28.81 0.55 575 201 204 3 742.32 4.72 576 201 205 4 724.25 1.89 577 201 206 5 706.59 3.00 578 201 209 8 720.39 3.81 579 202 206 4 707.56 2.51 580 204 208 4 744.13 1.03 581 204 228 24 43.95 0.14 582 205 208 3 740.88 2.66 583 205 209 4 738.03 1.23 584 205 226 21 45.01 0.03 585 205 228 23 44.26 0.29 586 206 209 3 742.38 3.40 587 206 210 4 731.34 1.47 588 207 210 3 739.86 0.32 589 207 211 4 731.34 1.47 590 208 211 3 745.21 0.57 591 208 212 4 731.33 1.47 592 208 219 11 66.07 0.83 593 209 212 3 733.09 6.90 594 209 213 4 721.82 3.29 595 209 219 10 58.84 0.80 596 210 213 3 747.35 2.57 597 212 215 3 722.69 3.60 598 212 216 4 685.66 1.55 599 212 219 7 630.58 11.95 600 213 216 3 748.97 4.15 601 215 219 4 747.58 1.61 602 216 219 3 741.26 2.66 603 216 220 4 716.25 1.26 604 217 220 3 732.26 9.27 605 221 224 3 765.34 2.68 606 221 226 5 737.86 3.19