BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 10 145 135 86.00 0.18 2 11 14 3 743.02 3.26 3 11 15 4 735.77 3.73 4 11 74 63 81.20 0.14 5 11 167 156 95.15 0.14 6 12 15 3 746.39 1.58 7 12 16 4 735.78 3.73 8 13 16 3 737.85 2.69 9 13 17 4 733.69 0.61 10 13 165 152 100.11 0.35 11 13 167 154 100.12 0.35 12 14 17 3 746.38 1.57 13 14 18 4 732.38 2.00 14 14 74 60 100.15 0.33 15 14 75 61 100.15 0.33 16 14 76 62 100.17 0.33 17 14 167 153 100.14 0.34 18 14 168 154 100.14 0.33 19 15 18 3 745.06 2.23 20 15 76 61 101.03 0.04 21 16 19 3 755.72 3.60 22 17 20 3 755.72 3.60 23 17 37 20 316.35 0.49 24 17 168 151 107.36 1.35 25 18 36 18 330.47 0.99 26 18 37 19 316.10 0.36 27 18 38 20 316.43 0.53 28 18 76 58 306.08 3.89 29 18 145 127 317.68 0.65 30 19 36 17 331.50 1.28 31 20 36 16 333.39 1.54 32 20 37 17 332.50 1.51 33 20 89 69 321.89 1.56 34 20 91 71 319.51 0.35 35 21 34 13 333.61 1.56 36 21 35 14 333.55 1.56 37 21 36 15 333.48 1.56 38 21 91 70 328.30 0.57 39 21 92 71 330.01 0.67 40 22 34 12 333.67 1.55 41 22 35 13 333.61 1.57 42 22 37 15 333.54 1.55 43 22 40 18 333.50 1.57 44 22 52 30 331.03 0.71 45 22 89 67 328.54 0.49 46 22 92 70 330.54 0.92 47 23 31 8 437.83 53.38 48 23 33 10 333.81 1.57 49 23 52 29 332.26 1.08 50 23 53 30 330.57 0.94 51 23 90 67 330.57 0.95 52 23 91 68 330.60 0.94 53 23 92 69 330.63 0.94 54 23 96 73 330.60 0.95 55 23 97 74 330.62 0.94 56 24 31 7 437.92 53.36 57 24 32 8 437.42 53.18 58 24 33 9 333.83 1.57 59 24 34 10 333.80 1.56 60 24 35 11 333.78 1.56 61 24 40 16 333.62 1.57 62 24 50 26 333.62 1.57 63 24 51 27 333.48 1.54 64 24 52 28 332.98 1.44 65 25 29 4 628.28 4.86 66 25 30 5 558.54 6.48 67 25 31 6 437.92 53.36 68 25 32 7 437.86 53.36 69 25 51 26 333.62 1.58 70 25 52 27 333.52 1.54 71 25 53 28 333.42 1.52 72 25 150 125 333.50 1.54 73 26 29 3 732.63 2.25 74 26 30 4 638.12 9.78 75 26 32 6 438.00 53.39 76 26 51 25 333.68 1.60 77 26 150 124 333.67 1.60 78 27 51 24 333.69 1.60 79 27 136 109 333.68 1.60 80 27 150 123 333.67 1.60 81 27 225 198 45.41 0.77 82 27 227 200 44.98 0.93 83 28 225 197 45.41 0.76 84 28 227 199 45.04 0.94 85 29 53 24 333.57 1.57 86 29 134 105 333.55 1.59 87 29 150 121 333.55 1.59 88 29 152 123 333.56 1.58 89 29 225 196 45.21 0.85 90 31 53 22 333.50 1.57 91 31 97 66 333.28 1.56 92 34 92 58 329.74 0.62 93 35 39 4 735.18 0.93 94 35 40 5 726.30 0.03 95 35 50 15 346.06 4.55 96 36 39 3 737.01 1.83 97 36 40 4 735.18 0.93 98 37 40 3 742.97 2.57 99 37 41 4 735.18 0.93 100 37 89 52 334.70 1.34 101 38 41 3 748.95 0.42 102 38 42 4 735.18 0.93 103 38 145 107 367.67 0.74 104 39 42 3 748.95 0.42 105 39 45 6 675.49 4.25 106 39 50 11 570.62 20.21 107 40 46 6 714.75 2.98 108 40 50 10 568.49 21.28 109 40 52 12 362.62 2.08 110 41 46 5 735.75 6.48 111 41 52 11 489.37 60.83 112 41 145 104 372.81 0.34 113 41 146 105 367.04 0.42 114 41 147 106 367.00 0.41 115 42 45 3 746.25 1.24 116 42 46 4 738.70 5.01 117 42 144 102 374.39 0.06 118 42 145 103 373.77 0.03 119 43 46 3 741.24 3.75 120 43 47 4 735.78 6.49 121 43 140 97 374.62 0.19 122 43 142 99 380.87 2.87 123 43 143 100 380.88 2.87 124 43 144 101 374.76 0.18 125 43 145 102 374.23 0.02 126 43 146 103 374.38 0.07 127 44 47 3 741.27 3.77 128 44 48 4 741.27 3.77 129 45 48 3 753.01 2.10 130 45 49 4 740.31 4.25 131 45 50 5 715.87 2.44 132 46 49 3 745.81 1.49 133 46 50 4 709.09 0.96 134 46 138 92 382.98 2.52 135 46 146 100 383.00 2.52 136 46 147 101 383.00 2.52 137 46 148 102 382.29 2.17 138 49 148 99 493.13 1.35 139 50 148 98 493.18 1.36 140 51 136 85 493.17 1.36 141 51 148 97 493.18 1.36 142 51 149 98 493.18 1.37 143 51 150 99 493.19 1.38 144 52 147 95 493.31 1.36 145 52 148 96 493.28 1.36 146 52 149 97 493.23 1.36 147 52 150 98 493.20 1.38 148 53 89 36 528.44 18.95 149 53 90 37 528.38 18.91 150 53 99 46 528.73 19.10 151 53 105 52 528.48 18.97 152 53 150 97 493.23 1.37 153 53 152 99 493.20 1.38 154 54 88 34 528.70 19.05 155 54 89 35 528.48 18.97 156 54 90 36 528.45 18.94 157 54 149 95 493.31 1.36 158 54 150 96 493.28 1.36 159 54 151 97 493.23 1.36 160 54 152 98 493.21 1.37 161 55 86 31 529.12 19.05 162 55 87 32 528.90 19.04 163 55 88 33 528.80 19.04 164 55 89 34 528.73 19.04 165 55 90 35 528.76 19.05 166 55 105 50 528.69 19.05 167 55 108 53 528.69 19.05 168 55 109 54 528.40 18.93 169 55 112 57 528.51 18.93 170 55 151 96 493.26 1.36 171 55 152 97 493.22 1.36 172 55 154 99 493.19 1.38 173 56 84 28 529.13 19.07 174 56 86 30 529.12 19.05 175 56 87 31 528.91 19.04 176 56 151 95 493.32 1.36 177 56 152 96 493.28 1.36 178 56 153 97 493.23 1.36 179 56 154 98 493.22 1.36 180 56 197 141 170.77 1.59 181 57 84 27 529.16 19.07 182 57 85 28 529.16 19.07 183 57 86 29 529.14 19.06 184 57 109 52 528.69 19.02 185 57 113 56 528.59 18.89 186 57 154 97 493.23 1.36 187 57 156 99 493.18 1.38 188 58 66 8 529.25 19.09 189 58 84 26 529.17 19.07 190 58 85 27 529.17 19.07 191 58 153 95 493.34 1.36 192 58 154 96 493.26 1.37 193 58 155 97 493.21 1.37 194 58 156 98 493.20 1.37 195 58 198 140 170.94 1.65 196 58 201 143 162.59 1.03 197 58 206 148 147.07 1.03 198 59 66 7 529.25 19.09 199 59 67 8 529.23 19.09 200 59 85 26 529.23 19.08 201 59 119 60 517.29 13.22 202 59 156 97 493.20 1.37 203 59 158 99 493.15 1.37 204 59 160 101 430.63 1.02 205 59 174 115 269.52 1.51 206 60 65 5 546.62 10.55 207 60 66 6 529.26 19.09 208 60 155 95 493.33 1.36 209 60 156 96 493.25 1.38 210 60 157 97 493.18 1.38 211 60 158 98 493.18 1.38 212 60 206 146 156.37 0.76 213 60 210 150 144.71 1.30 214 60 213 153 113.31 0.69 215 61 64 3 732.85 7.79 216 61 65 4 683.11 23.14 217 61 158 97 493.19 1.39 218 61 159 98 493.13 1.38 219 61 174 113 270.40 1.38 220 62 159 97 493.23 1.38 221 64 210 146 146.87 1.08 222 64 213 149 138.88 1.36 223 65 174 109 270.27 1.39 224 65 175 110 269.24 1.53 225 65 176 111 269.06 1.48 226 65 179 114 257.74 1.37 227 65 210 145 146.88 1.11 228 66 173 107 271.81 0.69 229 66 174 108 269.38 1.56 230 66 175 109 269.22 1.53 231 66 180 114 229.33 1.17 232 66 198 132 171.55 1.62 233 66 206 140 159.03 0.55 234 66 210 144 145.53 0.90 235 67 85 18 530.53 18.64 236 67 117 50 526.54 16.59 237 67 162 95 392.56 0.03 238 67 172 105 272.19 0.51 239 67 173 106 270.68 1.23 240 67 174 107 269.23 1.53 241 68 83 15 531.13 18.61 242 68 84 16 530.79 18.57 243 68 172 104 271.81 0.69 244 68 173 105 270.39 1.37 245 68 175 107 269.23 1.53 246 68 180 112 227.55 0.28 247 68 183 115 172.56 1.37 248 68 198 130 171.47 1.61 249 69 82 13 531.38 18.53 250 69 83 14 531.27 18.57 251 69 84 15 531.25 18.56 252 69 85 16 530.81 18.58 253 69 116 47 528.13 17.20 254 69 169 100 280.88 0.30 255 69 171 102 278.89 0.58 256 69 172 103 270.69 1.25 257 70 80 10 532.98 18.59 258 70 81 11 532.53 18.64 259 70 82 12 531.42 18.50 260 70 169 99 280.88 0.30 261 70 170 100 280.83 0.33 262 70 171 101 279.68 0.28 263 71 75 4 721.18 0.10 264 71 77 6 625.08 5.08 265 71 78 7 545.50 12.50 266 71 79 8 533.22 18.59 267 71 80 9 532.98 18.59 268 71 81 10 532.96 18.60 269 71 83 12 532.10 18.68 270 71 168 97 281.38 0.25 271 71 169 98 280.88 0.30 272 71 170 99 280.99 0.26 273 71 195 124 171.80 1.49 274 71 197 126 171.81 1.49 275 72 75 3 753.84 3.08 276 72 79 7 545.50 12.50 277 72 80 8 533.22 18.59 278 72 166 94 282.00 0.28 279 72 167 95 281.94 0.26 280 72 168 96 281.39 0.24 281 72 169 97 281.09 0.21 282 72 170 98 281.08 0.21 283 73 79 6 545.49 12.49 284 73 80 7 533.25 18.61 285 73 170 97 281.35 0.23 286 74 167 93 281.94 0.30 287 75 79 4 551.93 9.35 288 75 168 93 281.94 0.31 289 76 145 69 494.26 1.14 290 77 141 64 494.25 1.15 291 77 145 68 494.24 1.15 292 78 81 3 749.13 4.07 293 78 139 61 494.24 1.14 294 78 140 62 494.25 1.14 295 78 141 63 494.24 1.15 296 78 147 69 494.21 1.15 297 78 195 117 171.18 1.47 298 78 196 118 171.23 1.50 299 78 197 119 171.37 1.56 300 79 193 114 172.11 1.35 301 80 170 90 281.07 0.21 302 80 193 113 172.11 1.36 303 81 193 112 172.10 1.36 304 81 194 113 172.05 1.36 305 81 195 114 171.80 1.49 306 82 183 101 172.51 1.39 307 82 192 110 172.17 1.37 308 82 193 111 172.12 1.37 309 82 194 112 172.05 1.36 310 82 195 113 172.05 1.36 311 83 87 4 767.94 2.76 312 83 195 112 171.55 1.58 313 83 197 114 171.47 1.61 314 83 198 115 170.84 1.61 315 84 87 3 809.92 0.22 316 84 197 113 171.41 1.58 317 84 198 114 170.81 1.61 318 85 113 28 534.08 16.16 319 85 117 32 522.82 10.53 320 86 112 26 534.24 16.09 321 86 113 27 533.88 16.07 322 86 116 30 527.21 12.57 323 87 197 110 170.46 1.45 324 88 112 24 534.16 16.00 325 89 108 19 534.74 16.14 326 89 111 22 534.74 16.13 327 90 96 6 590.15 3.48 328 90 105 15 534.75 16.16 329 90 108 18 534.70 16.16 330 91 96 5 657.89 4.95 331 91 111 20 535.69 15.68 332 92 97 5 661.10 5.94 333 93 96 3 742.75 2.32 334 93 97 4 717.67 0.52 335 94 97 3 749.15 0.88 336 94 98 4 726.39 0.24 337 95 98 3 755.66 2.38 338 95 104 9 544.86 13.58 339 95 107 12 544.82 13.58 340 96 104 8 544.84 13.60 341 96 107 11 544.77 13.62 342 96 108 12 544.75 13.62 343 96 111 15 544.75 13.61 344 98 103 5 599.60 5.26 345 98 104 6 544.95 13.62 346 98 107 9 565.19 3.51 347 99 102 3 708.42 2.56 348 100 103 3 752.10 4.21 349 101 106 5 725.90 1.02 350 102 105 3 750.65 4.93 351 102 106 4 722.91 0.47 352 102 131 29 503.55 3.50 353 102 152 50 493.50 1.31 354 103 106 3 746.63 11.38 355 103 107 4 722.91 0.48 356 103 126 23 504.62 3.71 357 104 107 3 734.32 3.14 358 104 108 4 722.91 0.48 359 105 108 3 734.32 3.15 360 105 109 4 725.93 1.03 361 105 152 47 493.44 1.32 362 105 154 49 493.31 1.35 363 106 109 3 733.36 2.67 364 106 110 4 727.54 0.24 365 106 126 20 504.62 3.72 366 106 127 21 504.63 3.72 367 106 129 23 503.99 3.70 368 106 154 48 493.34 1.34 369 107 110 3 753.18 5.93 370 107 111 4 730.96 1.94 371 107 126 19 504.65 3.71 372 108 111 3 744.32 3.06 373 108 112 4 729.10 2.87 374 109 112 3 746.86 1.79 375 109 113 4 731.46 1.69 376 109 129 20 504.29 3.86 377 109 154 45 493.31 1.34 378 110 113 3 748.76 0.98 379 110 114 4 731.47 1.69 380 110 121 11 505.52 4.04 381 110 124 14 504.90 3.80 382 110 125 15 504.91 3.81 383 110 126 16 504.89 3.80 384 110 129 19 504.59 3.71 385 111 114 3 749.45 4.05 386 111 115 4 729.07 3.39 387 111 121 10 505.51 4.04 388 112 115 3 731.48 1.70 389 112 116 4 722.47 2.81 390 112 168 56 280.38 0.12 391 113 117 4 699.51 3.29 392 113 118 5 699.50 3.29 393 113 119 6 589.28 8.51 394 113 124 11 505.45 4.06 395 114 118 4 653.01 2.03 396 114 119 5 573.44 0.58 397 114 121 7 516.02 9.21 398 115 165 50 281.41 0.23 399 116 164 48 281.44 0.23 400 116 165 49 281.40 0.22 401 116 168 52 280.41 0.12 402 117 162 45 332.50 25.50 403 117 163 46 282.03 0.29 404 117 164 47 281.44 0.23 405 117 169 52 280.81 0.34 406 118 163 45 281.85 0.28 407 118 164 46 281.70 0.35 408 119 123 4 743.86 0.82 409 119 124 5 731.86 5.17 410 119 160 41 420.64 3.66 411 119 161 42 406.72 0.34 412 119 162 43 389.92 1.27 413 120 123 3 756.45 4.51 414 120 124 4 743.86 0.82 415 120 161 41 409.73 1.80 416 121 124 3 762.93 1.34 417 121 125 4 736.10 3.06 418 122 125 3 757.99 1.23 419 123 128 5 713.23 1.76 420 123 158 35 493.15 1.36 421 123 160 37 428.99 0.51 422 123 161 38 421.22 0.46 423 124 128 4 703.12 6.81 424 124 129 5 692.35 2.77 425 124 156 32 493.29 1.33 426 124 160 36 426.00 0.89 427 125 128 3 750.09 1.39 428 125 129 4 729.16 6.21 429 126 129 3 739.95 0.81 430 128 156 28 493.31 1.35 431 128 158 30 493.24 1.34 432 129 154 25 493.48 1.31 433 129 155 26 493.38 1.34 434 129 156 27 493.27 1.33 435 130 154 24 493.49 1.31 436 130 155 25 493.38 1.34 437 131 152 21 493.59 1.31 438 131 153 22 493.54 1.32 439 131 154 23 493.48 1.31 440 131 222 91 48.51 0.18 441 132 153 21 493.56 1.31 442 132 155 23 493.49 1.31 443 132 209 77 139.23 1.29 444 132 216 84 64.66 1.54 445 132 220 88 48.38 0.10 446 132 221 89 48.38 0.11 447 132 222 90 48.36 0.11 448 133 152 19 493.69 1.31 449 133 153 20 493.59 1.31 450 133 209 76 139.59 1.22 451 133 221 88 48.30 0.07 452 133 224 91 47.81 0.09 453 133 226 93 45.02 0.94 454 134 150 16 494.25 1.08 455 134 151 17 494.06 1.15 456 134 152 18 493.69 1.31 457 134 222 88 48.27 0.07 458 134 223 89 48.22 0.04 459 134 225 91 45.02 0.94 460 134 226 92 45.02 0.94 461 135 150 15 494.25 1.08 462 135 151 16 494.21 1.08 463 135 153 18 494.07 1.14 464 135 201 66 159.64 0.30 465 135 205 70 147.06 1.02 466 135 226 91 45.00 0.93 467 135 228 93 39.48 0.15 468 136 148 12 494.55 1.11 469 136 149 13 494.31 1.09 470 136 150 14 494.25 1.08 471 136 225 89 45.02 0.94 472 136 226 90 45.00 0.93 473 136 227 91 39.77 2.21 474 136 228 92 32.22 0.29 475 137 147 10 494.82 1.04 476 137 148 11 494.55 1.11 477 137 149 12 494.47 1.07 478 137 151 14 494.31 1.08 479 137 196 59 170.83 1.57 480 137 197 60 170.52 1.42 481 137 200 63 159.66 0.30 482 137 228 91 27.25 0.14 483 138 147 9 494.82 1.04 484 138 148 10 494.55 1.11 485 139 146 7 494.89 1.04 486 139 147 8 494.86 1.04 487 139 196 57 170.80 1.55 488 140 145 5 538.56 20.79 489 140 146 6 494.89 1.04 490 141 145 4 618.26 4.21 491 141 146 5 523.26 13.14 492 142 145 3 738.87 2.29 493 147 197 50 170.52 1.42 494 150 225 75 45.02 0.94 495 150 227 77 44.77 0.83 496 153 201 48 159.85 0.20 497 153 209 56 139.59 1.21 498 155 209 54 139.63 1.24 499 155 213 58 106.87 0.17 500 155 216 61 70.72 0.24 501 157 213 56 107.48 0.12 502 157 214 57 106.88 0.18 503 160 174 14 270.73 1.27 504 162 172 10 277.91 1.27 505 164 169 5 612.91 6.68 506 164 172 8 400.00 61.11 507 165 168 3 736.56 0.86 508 165 169 4 706.93 5.46 509 166 169 3 724.82 3.81 510 169 172 3 819.70 0.72 511 175 179 4 699.08 4.94 512 175 180 5 693.84 3.14 513 175 210 35 147.00 1.06 514 176 179 3 744.26 4.21 515 176 180 4 724.38 0.53 516 176 210 34 147.00 1.06 517 177 180 3 749.84 1.42 518 177 181 4 732.22 1.35 519 177 207 30 159.59 0.29 520 177 210 33 147.00 1.06 521 177 211 34 147.00 1.06 522 178 181 3 732.22 1.35 523 178 182 4 727.13 3.91 524 179 182 3 738.80 3.96 525 179 183 4 727.17 3.91 526 180 183 3 738.80 3.96 527 180 184 4 735.98 0.49 528 180 198 18 172.12 1.40 529 180 206 26 159.74 0.26 530 180 207 27 159.60 0.29 531 180 210 30 147.02 1.06 532 181 184 3 738.20 0.28 533 181 185 4 731.81 3.47 534 181 207 26 159.66 0.30 535 182 185 3 745.59 0.56 536 182 186 4 737.10 0.83 537 183 186 3 742.64 2.05 538 183 187 4 729.53 2.96 539 183 192 9 365.20 97.84 540 183 198 15 172.22 1.43 541 184 187 3 741.11 2.82 542 184 188 4 726.50 4.50 543 184 194 10 344.57 87.53 544 184 198 14 172.21 1.42 545 184 202 18 170.90 1.60 546 184 207 23 159.63 0.32 547 185 188 3 747.25 0.26 548 185 189 4 727.36 4.06 549 186 189 3 743.53 2.12 550 186 190 4 727.36 4.06 551 186 191 5 633.58 6.50 552 186 192 6 585.93 12.53 553 187 190 3 735.50 9.00 554 187 192 5 391.39 110.89 555 187 194 7 363.93 97.18 556 194 198 4 732.73 1.61 557 194 199 5 713.14 3.32 558 195 198 3 741.99 6.24 559 195 199 4 722.70 6.62 560 196 199 3 732.73 1.61 561 196 200 4 727.64 3.93 562 197 200 3 734.73 0.39 563 197 201 4 730.11 2.69 564 198 201 3 737.12 0.02 565 198 202 4 717.21 1.35 566 198 206 8 663.28 3.02 567 199 202 3 750.64 2.34 568 199 203 4 724.23 4.87 569 200 203 3 741.57 1.45 570 200 204 4 715.32 2.31 571 200 228 28 31.02 0.30 572 201 204 3 727.25 3.33 573 201 205 4 711.30 4.29 574 201 206 5 692.94 4.13 575 201 209 8 671.01 6.83 576 202 206 4 689.44 2.38 577 202 207 5 690.46 2.89 578 204 208 4 746.43 4.28 579 204 228 24 44.91 0.97 580 205 208 3 746.36 4.25 581 205 209 4 730.08 0.01 582 205 226 21 45.88 0.70 583 205 228 23 45.00 0.94 584 206 209 3 753.61 0.62 585 206 210 4 725.37 4.14 586 207 210 3 725.86 4.38 587 207 211 4 725.38 4.13 588 208 211 3 754.23 0.94 589 208 212 4 736.61 9.75 590 208 219 11 63.22 1.47 591 209 212 3 745.69 5.21 592 209 213 4 725.38 4.13 593 209 216 7 318.44 121.47 594 209 219 10 63.15 1.46 595 210 213 3 744.02 6.04 596 212 215 3 731.87 0.92 597 212 216 4 676.24 2.08 598 212 219 7 317.91 121.87 599 213 216 3 751.82 1.44 600 216 219 3 725.47 4.30 601 216 220 4 724.74 3.95 602 217 220 3 741.32 1.55 603 221 224 3 770.20 5.40 604 221 226 5 727.96 5.58