BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 202 199 145.88 13.18 2 3 203 200 145.88 13.18 3 3 204 201 143.06 11.85 4 3 223 220 119.07 0.21 5 3 224 221 118.91 0.28 6 3 226 223 118.80 0.28 7 4 35 31 539.63 27.57 8 4 36 32 539.55 27.57 9 4 37 33 539.53 27.57 10 4 204 200 145.60 13.04 11 5 35 30 539.75 27.57 12 5 36 31 539.60 27.55 13 5 37 32 539.55 27.57 14 5 220 215 119.03 0.21 15 5 226 221 118.86 0.28 16 5 227 222 118.84 0.27 17 5 228 223 118.84 0.27 18 5 245 240 38.34 0.68 19 5 246 241 28.50 0.82 20 6 37 31 539.60 27.55 21 6 39 33 539.55 27.57 22 6 160 154 216.24 48.14 23 6 204 198 152.66 16.37 24 6 206 200 145.59 13.04 25 6 226 220 119.11 0.21 26 6 227 221 118.84 0.27 27 6 228 222 118.84 0.27 28 7 36 29 539.85 27.57 29 7 37 30 539.71 27.55 30 7 38 31 539.56 27.57 31 7 39 32 539.56 27.57 32 7 206 199 152.51 16.34 33 7 227 220 119.10 0.21 34 7 228 221 118.85 0.27 35 7 230 223 118.82 0.27 36 7 239 232 56.69 0.62 37 7 242 235 45.98 0.06 38 7 243 236 38.39 0.66 39 8 39 31 539.56 27.57 40 8 206 198 152.78 16.41 41 8 228 220 119.09 0.21 42 8 229 221 118.84 0.27 43 8 230 222 118.82 0.27 44 8 239 231 56.73 0.62 45 9 39 30 539.72 27.54 46 9 40 31 539.71 27.54 47 9 41 32 539.71 27.54 48 9 230 221 118.84 0.27 49 9 234 225 77.65 0.39 50 9 239 230 56.71 0.63 51 10 41 31 539.76 27.55 52 10 63 53 539.75 27.56 53 10 91 81 539.53 27.46 54 10 94 84 524.53 19.96 55 10 229 219 119.89 0.01 56 10 230 220 119.09 0.21 57 10 231 221 118.84 0.27 58 10 234 224 81.83 1.12 59 11 14 3 855.11 2.21 60 11 20 9 542.06 27.82 61 11 23 12 542.06 27.82 62 11 24 13 542.06 27.82 63 11 40 29 540.23 27.50 64 11 41 30 540.22 27.50 65 11 42 31 540.21 27.48 66 11 43 32 540.32 27.48 67 11 50 39 540.71 27.58 68 11 63 52 540.19 27.48 69 11 234 223 113.02 0.15 70 11 235 224 81.82 1.11 71 12 43 31 540.36 27.46 72 12 63 51 540.19 27.48 73 12 94 82 533.78 24.38 74 12 96 84 522.86 18.79 75 12 231 219 119.91 0.01 76 13 43 30 540.38 27.46 77 14 43 29 540.86 27.69 78 14 235 221 118.67 0.32 79 15 19 4 816.20 0.24 80 15 46 31 541.82 27.84 81 16 19 3 847.41 0.86 82 16 20 4 828.09 0.55 83 16 46 30 541.97 27.85 84 17 20 3 845.67 1.66 85 17 21 4 844.41 2.28 86 17 46 29 542.07 27.80 87 17 49 32 542.44 27.85 88 18 21 3 849.81 0.41 89 18 22 4 845.57 1.71 90 19 22 3 845.57 1.71 91 19 23 4 834.61 3.77 92 20 23 3 840.76 0.70 93 20 24 4 834.60 3.77 94 20 46 26 542.87 27.80 95 20 49 29 543.46 27.64 96 20 50 30 543.20 27.76 97 21 24 3 862.03 8.59 98 21 25 4 834.37 3.89 99 22 25 3 839.03 0.02 100 22 26 4 839.03 0.02 101 23 26 3 854.03 6.87 102 23 27 4 839.96 0.48 103 23 234 211 114.64 0.96 104 23 235 212 79.42 1.17 105 24 27 3 844.35 2.54 106 24 28 4 839.95 0.48 107 24 50 26 543.75 27.68 108 24 53 29 543.89 27.73 109 24 54 30 543.85 27.71 110 25 28 3 865.02 1.36 111 25 29 4 831.88 0.29 112 26 29 3 840.08 0.56 113 26 30 4 823.27 3.21 114 26 235 209 83.03 2.94 115 26 236 210 77.65 0.39 116 26 237 211 64.86 1.72 117 26 240 214 57.23 0.42 118 27 30 3 869.22 3.46 119 27 32 5 752.34 27.99 120 27 38 11 544.20 27.89 121 27 58 31 543.98 27.78 122 27 239 212 59.34 1.47 123 27 243 216 54.52 0.67 124 28 53 25 545.20 27.48 125 28 55 27 544.84 27.64 126 30 240 210 56.70 0.62 127 31 240 209 56.73 0.61 128 31 244 213 46.07 0.07 129 32 58 26 548.81 25.81 130 32 240 208 56.69 0.62 131 32 243 211 46.06 0.06 132 32 244 212 45.88 0.07 133 33 36 3 858.25 3.29 134 33 243 210 46.38 0.10 135 33 244 211 40.18 0.23 136 33 247 214 36.62 1.46 137 36 243 207 40.18 0.23 138 37 89 52 648.95 27.53 139 37 204 167 152.76 16.39 140 38 58 20 685.60 45.80 141 38 59 21 685.60 45.80 142 38 239 201 56.73 0.64 143 38 243 205 39.34 0.19 144 39 58 19 685.63 45.79 145 39 59 20 685.60 45.80 146 39 61 22 685.62 45.80 147 39 89 50 649.02 27.55 148 39 123 84 539.86 25.39 149 40 50 10 685.78 45.76 150 40 58 18 685.71 45.80 151 40 59 19 685.68 45.80 152 40 60 20 685.64 45.79 153 40 61 21 685.65 45.79 154 41 61 20 685.65 45.79 155 41 63 22 685.62 45.77 156 41 91 50 649.09 27.50 157 42 46 4 822.27 2.08 158 42 47 5 716.24 30.70 159 42 50 8 685.98 45.78 160 42 60 18 685.76 45.77 161 42 61 19 685.70 45.78 162 42 63 21 685.65 45.77 163 43 46 3 858.85 3.02 164 43 47 4 825.93 6.32 165 43 63 20 685.71 45.76 166 44 47 3 842.03 0.66 167 44 77 33 650.03 27.90 168 46 49 3 859.96 6.09 169 46 50 4 833.88 2.00 170 47 50 3 863.22 5.34 171 47 51 4 834.00 1.97 172 47 60 13 686.37 45.59 173 47 61 14 686.24 45.66 174 47 62 15 686.13 45.62 175 47 77 30 650.41 27.73 176 47 82 35 649.96 27.50 177 47 85 38 650.24 27.36 178 47 87 40 650.03 27.34 179 48 51 3 844.05 1.85 180 48 52 4 835.94 0.99 181 48 85 37 650.23 27.36 182 49 52 3 848.28 0.48 183 49 53 4 843.99 1.67 184 50 53 3 848.28 0.48 185 50 54 4 833.99 1.94 186 50 60 10 686.69 45.44 187 51 54 3 860.05 0.23 188 51 55 4 860.05 0.23 189 51 56 5 828.02 1.75 190 51 58 7 723.45 32.61 191 51 60 9 686.69 45.44 192 51 85 34 650.66 27.13 193 51 86 35 650.39 27.14 194 51 87 36 650.33 27.14 195 51 88 37 650.33 27.14 196 54 58 4 814.77 5.72 197 54 60 6 687.02 45.47 198 55 58 3 849.05 1.27 199 59 88 29 677.41 13.46 200 59 89 30 677.37 13.46 201 60 87 27 677.54 13.46 202 60 88 28 677.53 13.47 203 60 89 29 677.40 13.46 204 61 89 28 677.39 13.45 205 61 90 29 677.38 13.45 206 61 91 30 677.38 13.45 207 62 77 15 679.04 13.35 208 62 82 20 677.88 13.40 209 62 89 27 677.55 13.46 210 62 90 28 677.45 13.42 211 62 91 29 677.45 13.42 212 63 91 28 677.45 13.42 213 64 77 13 679.13 13.38 214 64 90 26 677.79 13.36 215 64 91 27 677.55 13.42 216 64 92 28 677.49 13.40 217 64 97 33 637.91 6.55 218 64 111 47 573.04 39.50 219 65 77 12 679.12 13.38 220 65 78 13 679.06 13.36 221 65 79 14 679.05 13.37 222 65 82 17 679.03 13.35 223 65 90 25 677.82 13.36 224 65 92 27 677.75 13.37 225 65 111 46 573.11 39.50 226 65 115 50 572.12 40.00 227 66 73 7 679.29 13.38 228 66 76 10 679.22 13.39 229 66 77 11 679.19 13.39 230 66 79 13 679.11 13.37 231 66 107 41 573.18 39.48 232 66 111 45 573.12 39.51 233 66 114 48 573.12 39.52 234 67 107 40 573.16 39.49 235 67 110 43 573.14 39.51 236 67 111 44 573.13 39.51 237 67 114 47 573.12 39.53 238 68 73 5 835.56 8.71 239 68 78 10 679.24 13.39 240 68 114 46 573.16 39.51 241 69 78 9 679.23 13.39 242 69 114 45 573.27 39.56 243 73 76 3 846.48 1.64 244 77 81 4 833.07 1.21 245 77 82 5 833.06 1.21 246 78 81 3 862.18 2.55 247 78 82 4 835.87 2.61 248 79 82 3 837.05 2.12 249 79 83 4 835.87 2.61 250 79 114 35 572.66 40.37 251 79 115 36 572.73 40.33 252 79 118 39 572.12 40.64 253 79 120 41 572.03 40.68 254 80 83 3 843.47 0.83 255 80 84 4 834.11 0.63 256 81 84 3 840.25 2.44 257 81 85 4 834.12 0.64 258 82 85 3 865.58 0.99 259 82 86 4 834.12 0.64 260 82 87 5 742.11 18.96 261 82 89 7 705.24 0.62 262 82 90 8 690.62 7.13 263 82 120 38 597.31 53.40 264 83 86 3 840.59 2.61 265 83 87 4 810.64 4.41 266 83 118 35 597.52 53.34 267 83 119 36 597.43 53.38 268 83 120 37 597.35 53.41 269 87 120 33 640.12 74.92 270 88 120 32 639.98 74.98 271 88 121 33 639.96 75.00 272 89 120 31 639.98 74.99 273 89 121 32 639.90 75.02 274 89 122 33 639.89 75.03 275 89 123 34 639.84 75.06 276 89 160 71 238.30 57.23 277 90 115 25 640.89 74.55 278 90 120 30 640.00 74.98 279 90 121 31 639.98 75.00 280 90 122 32 639.87 75.04 281 90 123 33 639.77 75.09 282 91 94 3 915.61 2.51 283 91 123 32 639.77 75.09 284 91 125 34 639.71 75.11 285 91 229 138 119.89 0.00 286 92 111 19 641.74 74.18 287 92 112 20 641.11 74.49 288 92 115 23 641.10 74.48 289 92 122 30 639.94 75.03 290 92 123 31 639.88 75.05 291 92 124 32 639.61 75.17 292 92 125 33 639.61 75.17 293 93 98 5 796.45 0.98 294 93 108 15 641.78 74.16 295 93 125 32 639.59 75.17 296 94 97 3 842.95 0.66 297 94 98 4 831.64 2.02 298 94 125 31 639.60 75.18 299 94 164 70 241.00 55.43 300 94 229 135 121.28 0.62 301 95 98 3 837.75 1.03 302 95 99 4 817.06 9.31 303 95 164 69 240.86 55.36 304 95 166 71 238.81 55.19 305 96 100 4 796.48 0.97 306 96 101 5 796.44 0.99 307 97 101 4 795.27 1.54 308 97 102 5 795.27 1.54 309 97 103 6 795.16 1.51 310 98 102 4 795.21 1.52 311 98 103 5 795.16 1.50 312 98 105 7 643.47 74.34 313 98 108 10 643.46 74.34 314 98 125 27 639.74 75.14 315 98 126 28 639.69 75.16 316 98 145 47 247.45 55.27 317 99 102 3 819.02 0.58 318 99 129 30 638.62 75.45 319 99 166 67 242.82 54.38 320 103 107 4 842.20 3.30 321 103 108 5 836.52 4.03 322 103 111 8 805.17 0.42 323 104 107 3 855.44 7.90 324 104 108 4 842.19 3.29 325 105 108 3 855.07 6.27 326 105 109 4 837.81 3.45 327 105 144 39 247.62 55.26 328 105 145 40 247.44 55.27 329 105 148 43 246.97 55.15 330 106 109 3 843.76 0.62 331 106 110 4 840.28 2.34 332 107 110 3 857.48 6.19 333 107 111 4 837.95 3.52 334 108 111 3 851.55 3.27 335 108 112 4 837.95 3.52 336 108 149 41 245.79 55.33 337 109 112 3 851.31 2.22 338 109 113 4 837.94 3.52 339 109 148 39 239.37 58.55 340 109 149 40 239.03 58.54 341 109 152 43 238.82 58.43 342 110 113 3 842.37 1.30 343 110 114 4 840.02 2.44 344 111 114 3 843.81 0.55 345 111 115 4 837.93 3.47 346 112 115 3 846.26 0.20 347 112 116 4 837.91 3.44 348 112 122 10 640.01 75.00 349 112 124 12 639.96 75.00 350 112 149 37 239.78 58.10 351 112 153 41 238.48 57.66 352 113 116 3 852.28 1.18 353 113 117 4 838.18 3.59 354 113 152 39 237.94 57.97 355 114 117 3 844.33 3.45 356 114 118 4 837.05 3.73 357 115 118 3 845.92 2.36 358 115 119 4 837.51 3.50 359 115 120 5 800.62 1.67 360 115 122 7 656.77 66.72 361 116 119 3 851.95 1.62 362 116 120 4 811.91 3.97 363 116 122 6 656.79 66.72 364 116 153 37 238.59 57.58 365 116 155 39 237.84 57.73 366 116 158 42 236.57 58.13 367 116 159 43 237.11 57.88 368 121 158 37 237.16 57.80 369 122 158 36 238.36 57.24 370 122 159 37 237.40 57.69 371 122 160 38 238.03 57.37 372 123 160 37 238.03 57.36 373 123 162 39 238.27 57.15 374 123 206 83 156.41 16.55 375 123 229 106 119.53 0.04 376 124 149 25 243.40 56.26 377 124 160 36 238.44 57.18 378 124 161 37 238.34 57.19 379 124 162 38 238.38 57.13 380 125 162 37 238.36 57.11 381 125 164 39 241.62 55.38 382 125 229 104 120.91 0.47 383 126 142 16 247.18 55.34 384 126 145 19 247.17 55.30 385 126 146 20 247.12 55.33 386 126 161 35 241.82 55.48 387 126 162 36 241.81 55.48 388 126 163 37 241.65 55.40 389 126 164 38 243.74 54.34 390 126 187 61 171.06 18.00 391 127 141 14 247.53 55.30 392 127 142 15 247.33 55.34 393 127 145 18 247.44 55.27 394 127 163 36 243.75 54.35 395 127 164 37 243.74 54.33 396 128 132 4 818.53 1.42 397 128 133 5 818.54 1.42 398 128 138 10 247.59 55.34 399 128 141 13 247.52 55.26 400 128 163 35 244.38 54.24 401 128 166 38 244.02 53.91 402 128 167 39 243.97 53.93 403 129 132 3 842.94 0.14 404 129 133 4 837.12 4.70 405 129 166 37 244.00 53.91 406 129 167 38 243.12 53.50 407 130 133 3 848.51 1.00 408 130 134 4 834.46 6.03 409 130 167 37 243.62 53.72 410 131 134 3 859.60 0.64 411 131 135 4 836.30 4.30 412 132 135 3 841.95 2.29 413 132 136 4 836.30 4.30 414 132 137 5 661.70 78.61 415 132 141 9 353.04 3.31 416 133 136 3 851.62 3.35 417 133 137 4 813.24 2.48 418 133 138 5 611.43 53.45 419 133 163 30 245.53 53.77 420 133 167 34 244.74 53.55 421 133 179 46 174.28 18.12 422 133 183 50 174.11 18.04 423 137 140 3 837.99 1.87 424 137 141 4 820.88 0.13 425 138 141 3 848.45 1.17 426 138 142 4 841.94 3.83 427 138 183 45 173.81 17.53 428 139 142 3 863.27 4.07 429 139 143 4 828.23 3.02 430 139 186 47 173.50 17.34 431 140 143 3 863.27 4.07 432 140 144 4 845.37 0.61 433 141 144 3 851.60 0.99 434 141 145 4 845.37 0.61 435 142 145 3 848.87 2.36 436 142 146 4 843.87 0.14 437 142 163 21 245.84 53.59 438 142 187 45 173.68 17.18 439 143 146 3 844.99 5.39 440 143 147 4 838.84 2.38 441 143 186 43 173.75 17.18 442 143 187 44 173.68 17.20 443 143 190 47 174.10 16.42 444 144 147 3 851.58 1.08 445 144 148 4 838.84 2.37 446 145 148 3 868.47 5.04 447 145 149 4 843.92 4.91 448 146 149 3 843.93 4.91 449 146 150 4 840.25 5.46 450 146 161 15 246.20 53.47 451 146 187 41 178.44 14.78 452 146 190 44 178.25 14.36 453 146 191 45 178.25 14.36 454 147 150 3 841.49 6.08 455 147 151 4 843.88 4.88 456 147 190 43 181.05 12.97 457 148 151 3 855.65 0.29 458 148 152 4 851.85 0.91 459 149 152 3 855.71 0.33 460 149 153 4 839.32 5.94 461 149 156 7 815.57 3.23 462 150 153 3 846.96 2.17 463 150 156 6 812.23 4.70 464 150 195 45 220.44 14.96 465 153 156 3 896.59 0.81 466 153 159 6 448.81 31.14 467 156 159 3 837.97 13.48 468 156 161 5 831.12 10.06 469 156 195 39 259.07 27.98 470 156 199 43 252.16 31.12 471 156 203 47 251.80 31.00 472 159 203 44 252.45 31.32 473 160 204 44 252.40 31.35 474 160 206 46 252.30 31.39 475 161 187 26 261.21 27.23 476 161 191 30 261.13 27.23 477 161 203 42 252.67 31.39 478 161 204 43 252.62 31.41 479 161 205 44 252.30 31.39 480 161 206 45 252.31 31.39 481 162 206 44 252.30 31.40 482 162 229 67 171.93 26.00 483 163 183 20 261.30 27.21 484 163 184 21 261.26 27.21 485 163 205 42 252.59 31.46 486 163 206 43 252.36 31.41 487 163 207 44 252.28 31.44 488 163 208 45 252.22 31.43 489 164 208 44 251.65 31.71 490 165 168 3 869.00 4.73 491 165 175 10 262.50 26.68 492 165 207 42 252.52 31.50 493 165 208 43 252.37 31.55 494 165 209 44 251.66 31.71 495 166 169 3 860.03 0.24 496 167 171 4 857.59 0.53 497 167 175 8 263.50 26.48 498 167 179 12 263.49 26.48 499 168 171 3 850.88 3.87 500 168 173 5 317.90 16.60 501 168 174 6 263.47 26.46 502 168 175 7 263.49 26.47 503 174 210 36 251.72 31.74 504 175 179 4 825.35 0.99 505 175 207 32 252.56 31.48 506 176 179 3 834.07 2.39 507 176 180 4 829.41 3.02 508 177 180 3 835.80 0.72 509 177 181 4 831.56 1.96 510 177 218 41 174.37 26.79 511 177 219 42 174.12 26.66 512 177 222 45 174.08 26.68 513 178 181 3 832.56 2.46 514 178 182 4 831.57 1.96 515 179 182 3 849.50 0.01 516 179 183 4 832.83 2.32 517 180 183 3 865.81 4.13 518 180 184 4 844.31 3.86 519 180 207 27 252.62 31.45 520 180 219 39 174.12 26.66 521 180 225 45 173.72 26.73 522 181 184 3 853.53 2.00 523 181 185 4 844.31 3.86 524 181 222 41 173.79 26.75 525 181 223 42 173.88 26.69 526 181 224 43 173.70 26.76 527 181 225 44 173.66 26.76 528 182 185 3 854.00 1.78 529 182 186 4 844.31 3.86 530 183 186 3 853.43 2.69 531 183 187 4 834.26 1.09 532 184 187 3 839.35 1.45 533 184 188 4 834.25 1.09 534 184 205 21 252.91 31.46 535 184 206 22 252.91 31.46 536 184 207 23 252.87 31.46 537 184 224 40 173.59 26.75 538 184 225 41 173.59 26.75 539 185 188 3 851.50 1.13 540 185 189 4 834.22 1.11 541 185 224 39 173.58 26.75 542 186 189 3 835.58 3.73 543 186 190 4 829.24 0.88 544 187 190 3 830.20 1.04 545 187 191 4 838.49 5.96 546 187 205 18 252.97 31.43 547 188 191 3 853.56 2.14 548 188 192 4 851.49 3.17 549 188 200 12 253.43 31.52 550 188 203 15 253.39 31.50 551 188 205 17 253.02 31.46 552 188 224 36 173.55 26.74 553 188 226 38 173.53 26.73 554 189 192 3 852.98 2.43 555 189 193 4 851.49 3.17 556 190 193 3 855.75 1.04 557 190 194 4 825.51 0.36 558 190 195 5 825.50 0.35 559 191 194 3 851.69 3.06 560 191 195 4 825.47 0.34 561 191 196 5 821.71 2.12 562 191 199 8 441.31 39.04 563 191 200 9 292.39 16.05 564 191 203 12 283.20 20.64 565 192 196 4 732.85 42.30 566 192 197 5 328.19 33.95 567 192 200 8 292.38 16.05 568 195 199 4 835.39 0.37 569 196 199 3 856.51 3.98 570 196 200 4 834.11 0.27 571 197 200 3 854.80 2.92 572 197 201 4 841.09 3.92 573 198 201 3 858.01 0.82 574 198 202 4 833.36 0.06 575 199 202 3 854.30 2.68 576 199 203 4 831.20 1.02 577 200 203 3 857.46 0.53 578 203 226 23 172.56 26.29 579 204 226 22 172.56 26.28 580 205 225 20 172.59 26.27 581 205 226 21 172.58 26.27 582 206 227 21 172.49 26.25 583 206 228 22 172.46 26.23 584 206 229 23 172.43 26.23 585 207 211 4 867.06 1.90 586 207 219 12 173.34 26.30 587 207 225 18 172.91 26.38 588 207 227 20 172.59 26.26 589 207 228 21 172.46 26.21 590 207 229 22 172.46 26.21 591 208 229 21 172.45 26.21 592 210 215 5 827.81 2.58 593 211 215 4 835.12 1.07 594 211 216 5 464.04 27.59 595 211 219 8 304.75 29.23 596 211 228 17 172.78 26.25 597 211 230 19 172.49 26.21 598 211 233 22 171.94 26.00 599 211 242 31 54.95 0.10 600 212 215 3 856.38 0.88 601 212 216 4 821.99 0.33 602 212 238 26 110.71 26.34 603 212 242 30 56.23 0.54 604 213 216 3 857.92 0.10 605 213 238 25 101.55 21.77 606 213 241 28 101.11 21.78 607 215 218 3 865.24 1.11 608 215 219 4 823.94 6.11 609 216 219 3 836.11 0.03 610 216 220 4 823.32 5.74 611 216 228 12 173.84 26.65 612 216 241 25 56.34 0.56 613 216 242 26 50.00 1.15 614 216 245 29 38.39 0.67 615 217 220 3 844.90 5.07 616 217 221 4 833.44 10.80 617 217 244 27 46.02 0.08 618 217 245 28 38.38 0.66 619 217 247 30 38.39 0.66 620 218 221 3 850.51 2.27 621 218 222 4 840.26 7.38 622 219 222 3 867.39 2.05 623 219 225 6 289.76 33.42 624 219 228 9 173.86 26.66 625 220 228 8 173.89 26.66 626 220 245 25 38.36 0.67 627 220 246 26 38.31 0.68 628 222 225 3 840.54 3.79 629 225 228 3 999.63 0.55 630 225 246 21 38.16 0.75 631 226 246 20 30.64 0.01 632 228 242 14 46.01 0.07 633 228 245 17 38.37 0.67 634 230 233 3 886.84 0.41 635 230 234 4 816.22 9.23 636 230 239 9 101.33 21.68 637 230 242 12 48.43 0.57 638 231 234 3 875.71 9.03 639 232 235 3 873.27 3.81 640 233 236 3 841.64 1.72 641 233 238 5 310.72 13.89 642 233 239 6 186.59 19.17 643 233 242 9 181.98 18.53 644 234 239 5 186.59 19.17 645 236 239 3 859.92 1.37 646 237 240 3 860.52 1.07 647 238 241 3 855.39 1.50 648 238 242 4 850.46 3.97 649 239 242 3 856.96 0.71 650 239 243 4 834.26 0.71 651 240 243 3 851.30 3.27 652 240 244 4 840.27 2.25 653 241 244 3 854.95 1.44 654 241 245 4 839.47 2.65 655 242 245 3 830.13 7.32 656 244 247 3 843.80 4.35