BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 672 675 3 826.72 1.15 2 672 676 4 827.62 0.50 3 673 676 3 845.64 5.45 4 673 677 4 840.64 1.47 5 673 723 50 31.85 0.03 6 673 806 133 30.41 0.72 7 673 834 161 30.41 0.72 8 673 837 164 30.41 0.72 9 674 677 3 841.46 1.06 10 674 678 4 838.15 2.71 11 674 802 128 32.12 0.10 12 674 841 167 32.11 0.10 13 675 678 3 842.53 0.53 14 675 679 4 838.14 2.71 15 676 679 3 838.15 2.71 16 676 680 4 836.07 3.75 17 676 721 45 38.87 0.96 18 676 723 47 35.48 0.91 19 677 680 3 860.62 1.03 20 677 681 4 826.50 1.07 21 677 722 45 36.67 0.38 22 677 723 46 36.20 0.61 23 677 805 128 32.78 0.14 24 677 806 129 32.78 0.14 25 677 809 132 32.80 0.15 26 678 681 3 858.91 0.30 27 678 805 127 34.68 1.09 28 680 718 38 39.08 0.89 29 680 721 41 39.09 0.90 30 680 722 42 39.07 0.89 31 681 718 37 39.15 0.91 32 681 805 124 38.79 0.85 33 681 808 127 38.92 0.83 34 682 714 32 40.55 0.38 35 682 718 36 39.21 0.90 36 684 766 82 89.73 0.27 37 685 707 22 89.92 0.33 38 685 710 25 89.26 0.42 39 685 711 26 79.13 0.05 40 685 714 29 81.22 0.28 41 685 765 80 89.55 0.21 42 685 766 81 89.94 0.27 43 686 707 21 89.98 0.31 44 686 710 24 89.98 0.32 45 686 765 79 89.91 0.27 46 686 766 80 89.97 0.28 47 686 767 81 89.99 0.28 48 687 703 16 90.07 0.34 49 687 706 19 90.06 0.33 50 687 707 20 89.98 0.31 51 687 710 23 90.03 0.32 52 687 765 78 89.99 0.29 53 687 767 80 90.07 0.29 54 687 768 81 90.01 0.28 55 688 710 22 90.18 0.35 56 688 767 79 90.41 0.40 57 689 693 4 874.25 4.40 58 689 700 11 90.44 0.42 59 689 703 14 90.44 0.42 60 689 767 78 90.44 0.41 61 689 768 79 90.40 0.40 62 689 774 85 90.67 0.34 63 690 693 3 871.13 3.08 64 691 767 76 90.67 0.39 65 691 774 83 108.61 3.12 66 693 699 6 778.49 4.96 67 695 700 5 823.18 4.95 68 695 773 78 131.65 0.58 69 695 774 79 131.21 0.69 70 695 777 82 131.69 0.59 71 696 699 3 838.66 1.76 72 696 700 4 829.98 1.55 73 696 777 81 131.73 0.60 74 697 700 3 844.98 2.67 75 697 701 4 830.00 1.54 76 697 777 80 131.74 0.60 77 697 778 81 131.74 0.60 78 697 779 82 131.74 0.60 79 697 876 179 134.62 2.04 80 697 880 183 145.38 0.06 81 697 887 190 148.17 0.68 82 697 889 192 148.15 0.67 83 698 701 3 858.57 0.36 84 698 702 4 830.75 1.91 85 698 888 190 148.18 0.68 86 698 889 191 148.08 0.69 87 699 702 3 850.75 3.55 88 699 703 4 831.94 1.32 89 700 703 3 857.59 7.44 90 700 704 4 832.01 1.28 91 700 768 68 148.93 0.60 92 700 770 70 148.91 0.60 93 700 774 74 148.90 0.61 94 700 777 77 149.01 0.65 95 700 778 78 148.92 0.60 96 701 704 3 846.67 8.61 97 701 705 4 831.95 1.31 98 701 778 77 149.02 0.65 99 701 876 175 148.90 0.61 100 701 880 179 148.97 0.63 101 701 889 188 152.98 0.09 102 702 705 3 842.38 6.46 103 702 706 4 836.00 3.34 104 702 889 187 153.00 0.09 105 702 890 188 153.99 0.58 106 703 706 3 842.38 6.46 107 703 707 4 823.79 2.71 108 703 768 65 155.38 0.96 109 704 707 3 838.37 4.57 110 704 708 4 834.61 2.69 111 704 764 60 155.27 1.02 112 704 768 64 156.77 0.29 113 704 770 66 156.76 0.29 114 704 780 76 155.76 0.80 115 705 708 3 845.51 4.83 116 705 709 4 843.43 5.88 117 705 889 184 170.46 0.33 118 705 890 185 170.46 0.34 119 705 891 186 169.82 0.59 120 705 892 187 170.43 0.35 121 705 895 190 160.35 1.23 122 706 709 3 843.97 1.79 123 706 710 4 840.26 1.25 124 706 892 186 171.50 0.18 125 707 710 3 855.03 5.20 126 707 711 4 840.26 1.25 127 707 764 57 167.22 0.42 128 707 765 58 167.27 0.42 129 707 768 61 167.41 0.35 130 708 711 3 862.37 3.73 131 708 712 4 840.26 1.25 132 708 741 33 167.28 0.41 133 708 895 187 173.69 0.58 134 709 712 3 862.00 2.90 135 709 713 4 840.26 1.18 136 709 892 183 176.91 0.88 137 709 893 184 181.51 1.40 138 709 894 185 175.37 0.11 139 709 895 186 173.97 0.56 140 710 713 3 874.38 2.33 141 710 714 4 840.25 1.18 142 711 714 3 862.57 3.58 143 711 715 4 834.30 0.55 144 711 741 30 175.55 0.17 145 711 745 34 175.35 0.10 146 711 752 41 175.41 0.13 147 712 715 3 844.40 4.51 148 712 716 4 834.30 0.55 149 712 737 25 179.68 0.88 150 712 741 29 175.56 0.16 151 712 894 182 185.21 2.19 152 712 895 183 184.97 2.25 153 712 898 186 184.85 2.30 154 713 716 3 856.39 1.48 155 713 717 4 838.47 2.68 156 713 894 181 187.80 0.97 157 714 717 3 856.95 6.57 158 714 718 4 838.15 2.83 159 715 718 3 844.12 0.14 160 715 719 4 835.98 3.93 161 715 737 22 185.29 2.18 162 715 741 26 185.02 2.26 163 715 744 29 184.97 2.24 164 715 745 30 184.97 2.24 165 716 719 3 848.41 2.00 166 716 720 4 840.30 6.07 167 716 734 18 192.81 1.99 168 716 737 21 187.79 0.97 169 717 720 3 849.72 1.36 170 717 721 4 840.29 6.07 171 718 721 3 853.41 3.21 172 718 722 4 840.28 6.07 173 718 744 26 194.02 1.54 174 718 808 90 193.92 1.50 175 718 809 91 193.92 1.50 176 718 812 94 194.05 1.55 177 719 722 3 864.88 2.53 178 719 725 6 580.31 13.64 179 719 737 18 194.09 1.58 180 719 812 93 194.02 1.54 181 720 725 5 571.64 16.48 182 720 726 6 532.25 10.38 183 720 733 13 194.74 1.70 184 720 734 14 194.74 1.71 185 720 737 17 194.69 1.68 186 722 725 3 841.16 1.59 187 722 809 87 194.87 1.69 188 722 812 90 266.09 33.40 189 722 813 91 277.40 39.05 190 722 834 112 355.57 0.48 191 723 830 107 355.87 0.42 192 723 834 111 355.68 0.47 193 724 727 3 846.84 3.25 194 724 830 106 355.89 0.41 195 725 728 3 869.46 0.04 196 725 733 8 526.21 4.58 197 725 736 11 496.29 3.17 198 725 737 12 496.26 3.19 199 725 812 87 355.87 0.42 200 725 813 88 355.90 0.41 201 725 830 105 359.39 1.32 202 726 733 7 531.28 2.49 203 727 826 99 390.06 1.86 204 727 830 103 389.72 1.99 205 728 733 5 792.72 7.15 206 728 736 8 792.80 7.10 207 728 813 85 389.79 1.98 208 728 826 98 390.63 1.57 209 728 827 99 390.04 1.85 210 728 830 102 390.06 1.86 211 729 732 3 839.24 6.29 212 729 733 4 833.08 5.03 213 729 822 93 397.51 1.97 214 729 823 94 397.50 1.98 215 730 733 3 839.96 1.59 216 731 734 3 861.21 1.14 217 731 735 4 836.85 5.09 218 732 735 3 844.07 3.28 219 732 736 4 836.75 5.14 220 732 821 89 398.78 2.33 221 732 822 90 398.82 2.35 222 732 823 91 408.54 0.55 223 733 736 3 868.48 0.41 224 733 737 4 840.23 3.40 225 734 737 3 852.40 3.60 226 734 738 4 836.72 5.12 227 735 738 3 854.10 3.57 228 735 739 4 836.72 5.12 229 736 739 3 854.61 3.82 230 736 740 4 832.03 7.46 231 736 812 76 408.11 0.33 232 736 821 85 418.95 0.32 233 736 827 91 418.98 0.39 234 737 740 3 852.73 3.60 235 737 741 4 815.23 2.91 236 737 812 75 414.50 0.77 237 738 741 3 835.45 5.04 238 738 898 160 271.33 0.96 239 738 902 164 269.26 0.07 240 739 815 76 419.00 0.33 241 739 817 78 419.02 0.32 242 739 902 163 269.39 0.01 243 739 905 166 269.25 0.07 244 739 906 167 264.15 1.49 245 739 911 172 260.98 0.56 246 740 743 3 850.59 5.08 247 740 744 4 830.50 0.41 248 740 811 71 418.98 0.46 249 740 812 72 419.01 0.44 250 740 815 75 419.01 0.33 251 741 744 3 832.08 1.19 252 741 745 4 830.50 0.41 253 741 895 154 295.19 2.98 254 742 745 3 842.98 1.30 255 742 746 4 838.45 3.57 256 742 870 128 418.28 0.68 257 742 873 131 394.20 4.57 258 742 874 132 369.07 1.64 259 742 877 135 350.88 1.06 260 743 746 3 840.57 0.10 261 743 747 4 836.91 1.77 262 743 811 68 425.34 1.71 263 743 866 123 418.90 0.45 264 743 870 127 418.54 0.60 265 744 747 3 838.52 2.57 266 744 748 4 836.86 1.75 267 744 808 64 425.77 1.59 268 744 811 67 425.56 1.64 269 744 812 68 425.64 1.66 270 745 748 3 844.27 0.49 271 745 749 4 836.86 1.75 272 746 749 3 859.28 0.63 273 746 750 4 840.38 8.82 274 746 790 44 439.38 0.09 275 746 866 120 425.44 1.45 276 746 869 123 423.80 1.70 277 746 870 124 423.60 1.71 278 746 873 127 421.65 0.79 279 747 750 3 856.96 0.54 280 747 751 4 845.08 6.47 281 747 790 43 439.43 0.07 282 747 794 47 439.38 0.04 283 747 804 57 433.72 0.89 284 747 807 60 433.62 0.85 285 747 808 61 433.62 0.85 286 747 811 64 433.53 0.86 287 747 862 115 428.44 2.79 288 747 866 119 425.62 1.48 289 748 751 3 842.50 7.77 290 748 752 4 840.36 8.82 291 748 804 56 433.68 0.88 292 748 808 60 433.53 0.86 293 749 752 3 844.26 6.87 294 749 753 4 828.90 3.09 295 749 762 13 441.12 0.53 296 749 763 14 441.10 0.53 297 749 764 15 440.89 0.42 298 749 787 38 439.61 0.02 299 750 753 3 859.25 0.63 300 750 754 4 832.52 1.28 301 750 791 41 439.39 0.04 302 750 794 44 439.40 0.04 303 751 754 3 844.85 7.44 304 751 755 4 830.27 2.41 305 751 794 43 439.40 0.04 306 751 799 48 435.22 1.01 307 751 800 49 435.25 1.02 308 751 801 50 435.19 0.99 309 751 804 53 433.65 0.86 310 752 755 3 844.83 7.45 311 752 756 4 815.09 3.46 312 752 763 11 441.14 0.52 313 752 764 12 441.16 0.51 314 753 756 3 844.58 0.63 315 753 757 4 807.65 6.28 316 753 758 5 798.85 1.88 317 753 759 6 532.17 5.43 318 753 761 8 492.61 25.15 319 753 762 9 461.16 9.46 320 753 763 10 441.14 0.53 321 753 791 38 439.53 0.05 322 754 758 4 666.14 32.57 323 754 759 5 553.13 4.67 324 754 791 37 439.53 0.05 325 754 794 40 439.44 0.06 326 754 795 41 439.42 0.06 327 756 763 7 477.41 17.54 328 757 760 3 849.24 3.10 329 757 761 4 789.81 21.59 330 757 763 6 598.13 27.59 331 759 788 29 439.64 0.03 332 759 791 32 439.64 0.03 333 759 792 33 439.56 0.04 334 761 769 8 461.83 9.95 335 761 770 9 441.39 0.26 336 761 771 10 441.37 0.25 337 762 769 7 461.83 9.96 338 762 770 8 441.38 0.26 339 762 784 22 439.68 0.02 340 762 787 25 439.66 0.03 341 762 788 26 439.63 0.01 342 763 766 3 905.16 5.23 343 763 769 6 462.34 9.71 344 764 768 4 790.99 3.25 345 764 770 6 614.13 31.16 346 765 768 3 826.16 4.24 347 765 769 4 806.89 4.71 348 766 769 3 892.55 4.89 349 769 774 5 820.90 1.37 350 770 774 4 811.51 3.32 351 770 775 5 811.44 3.35 352 770 778 8 794.20 11.78 353 770 784 14 468.23 13.96 354 771 774 3 849.37 4.23 355 771 775 4 837.37 4.26 356 772 775 3 847.53 0.79 357 772 776 4 838.57 3.67 358 772 781 9 537.93 1.92 359 772 788 16 468.31 14.00 360 773 776 3 847.52 0.80 361 773 777 4 837.75 1.40 362 774 777 3 829.34 2.81 363 774 778 4 827.40 1.84 364 775 778 3 843.18 1.29 365 775 781 6 529.88 2.10 366 775 784 9 468.42 13.98 367 775 785 10 487.28 23.41 368 775 788 13 487.26 23.41 369 776 779 3 843.19 1.29 370 776 781 5 564.54 15.22 371 778 876 98 428.55 0.40 372 779 876 97 429.18 0.70 373 780 783 3 846.04 3.48 374 780 784 4 822.10 4.89 375 780 873 93 430.75 0.07 376 780 876 96 428.56 0.40 377 781 784 3 842.29 2.82 378 781 785 4 825.00 3.44 379 782 785 3 837.41 0.91 380 782 786 4 831.37 6.35 381 783 786 3 847.38 8.25 382 783 787 4 831.37 6.35 383 783 872 89 430.75 0.06 384 783 876 93 428.84 0.54 385 784 787 3 840.73 7.04 386 784 788 4 840.13 1.97 387 785 788 3 845.50 4.65 388 785 789 4 840.13 1.97 389 786 789 3 840.14 1.97 390 786 790 4 832.39 1.90 391 786 868 82 431.49 0.00 392 786 869 83 431.35 0.06 393 786 872 86 430.92 0.12 394 787 790 3 832.39 1.90 395 787 791 4 834.08 1.05 396 787 869 82 431.30 0.06 397 787 873 86 430.72 0.07 398 788 791 3 839.02 2.96 399 788 792 4 844.62 1.09 400 789 792 3 845.88 0.47 401 789 793 4 845.89 0.47 402 790 793 3 855.53 4.88 403 790 794 4 850.23 1.70 404 790 862 72 433.59 0.81 405 790 865 75 432.69 0.60 406 790 866 76 431.68 0.07 407 790 869 79 431.34 0.06 408 791 794 3 859.46 2.92 409 792 795 3 845.38 0.72 410 792 796 4 836.20 3.85 411 793 796 3 842.32 0.80 412 793 797 4 816.84 5.78 413 793 862 69 433.67 0.84 414 794 798 4 795.82 3.29 415 794 799 5 577.27 19.46 416 794 804 10 533.50 2.43 417 794 862 68 433.66 0.85 418 795 798 3 827.05 3.39 419 797 855 58 466.01 6.50 420 797 858 61 458.51 2.76 421 797 862 65 443.43 3.20 422 798 855 57 467.72 5.75 423 799 803 4 833.31 1.19 424 799 804 5 816.15 6.24 425 799 807 8 763.36 1.81 426 799 855 56 473.03 5.28 427 799 859 60 466.43 6.36 428 799 862 63 466.12 6.21 429 800 803 3 851.24 1.21 430 800 804 4 833.32 1.19 431 801 804 3 852.15 0.59 432 801 805 4 833.32 1.19 433 802 805 3 852.16 0.58 434 802 806 4 843.32 3.83 435 802 841 39 476.47 6.38 436 803 806 3 853.02 1.02 437 803 807 4 841.18 2.78 438 803 841 38 476.48 6.38 439 803 855 52 475.59 5.96 440 803 859 56 474.67 5.75 441 804 807 3 850.90 2.08 442 804 808 4 844.91 0.91 443 805 808 3 844.92 0.90 444 805 809 4 837.89 2.60 445 806 809 3 830.19 1.39 446 806 810 4 830.11 1.34 447 806 834 28 477.34 6.57 448 806 837 31 477.02 6.52 449 806 838 32 476.61 6.40 450 806 841 35 476.54 6.37 451 807 810 3 834.05 0.62 452 807 811 4 834.05 0.62 453 807 838 31 476.95 6.57 454 807 859 52 474.87 5.83 455 807 862 55 474.86 5.83 456 807 863 56 474.63 5.73 457 808 811 3 857.50 1.85 458 808 812 4 838.36 1.53 459 809 812 3 870.07 0.38 460 809 813 4 855.38 3.26 461 809 834 25 477.39 6.57 462 810 813 3 853.96 3.97 463 810 814 4 851.65 5.12 464 810 831 21 477.94 6.35 465 810 834 24 477.39 6.56 466 810 835 25 477.41 6.58 467 810 838 28 477.34 6.57 468 810 863 53 474.88 5.83 469 811 814 3 851.65 5.12 470 811 863 52 474.85 5.83 471 811 866 55 474.70 5.82 472 811 867 56 474.62 5.80 473 813 827 14 478.26 6.30 474 813 830 17 478.24 6.29 475 813 831 18 478.17 6.28 476 813 834 21 478.16 6.29 477 813 914 101 295.98 2.00 478 814 831 17 478.20 6.27 479 814 867 53 474.79 5.82 480 814 913 99 296.00 2.00 481 814 914 100 295.95 2.02 482 815 906 91 301.03 2.04 483 815 911 96 299.14 1.61 484 815 912 97 297.25 2.12 485 815 913 98 296.27 1.86 486 815 914 99 296.26 1.86 487 816 821 5 703.46 23.35 488 816 824 8 485.32 6.12 489 816 827 11 482.02 5.30 490 816 911 95 299.22 1.66 491 816 912 96 299.08 1.60 492 816 914 98 297.36 2.18 493 817 820 3 806.94 16.30 494 817 821 4 719.73 18.60 495 817 910 93 300.04 1.95 496 817 911 94 299.26 1.63 497 818 909 91 300.50 1.96 498 818 910 92 300.04 1.95 499 818 911 93 300.03 1.95 500 818 912 94 299.94 1.91 501 820 824 4 767.41 8.59 502 821 824 3 800.96 10.88 503 821 827 6 693.78 6.08 504 823 826 3 871.45 0.39 505 823 827 4 836.26 1.68 506 824 827 3 836.26 1.68 507 824 828 4 835.38 2.19 508 825 828 3 842.26 1.25 509 825 829 4 835.38 2.19 510 826 829 3 846.50 3.37 511 826 830 4 835.24 2.25 512 827 830 3 837.44 1.16 513 827 831 4 835.22 2.25 514 827 914 87 297.86 2.07 515 828 831 3 850.53 4.39 516 828 832 4 829.45 0.64 517 829 832 3 837.99 1.02 518 829 833 4 821.34 5.16 519 830 833 3 837.99 1.02 520 830 834 4 828.68 4.25 521 831 834 3 840.55 0.26 522 831 835 4 822.40 5.69 523 831 914 83 296.53 2.26 524 831 916 85 295.99 2.02 525 832 835 3 849.85 1.03 526 832 836 4 822.40 5.69 527 833 836 3 855.38 1.74 528 833 837 4 831.46 10.22 529 834 837 3 852.26 0.18 530 834 838 4 831.18 10.37 531 835 838 3 842.51 4.70 532 835 839 4 815.34 2.46 533 835 856 21 476.68 6.41 534 835 916 81 295.73 2.11 535 836 839 3 838.47 6.87 536 836 840 4 829.87 6.22 537 837 840 3 854.95 1.38 538 837 841 4 822.13 0.63 539 838 841 3 860.10 6.42 540 838 842 4 829.25 3.17 541 838 856 18 476.69 6.42 542 838 859 21 476.54 6.33 543 838 863 25 476.39 6.36 544 839 842 3 848.09 0.98 545 839 843 4 821.25 0.84 546 839 856 17 476.74 6.41 547 840 843 3 834.96 6.02 548 840 844 4 821.24 0.84 549 841 844 3 847.76 1.48 550 842 846 4 815.44 2.66 551 842 852 10 477.04 6.36 552 842 855 13 476.99 6.36 553 842 856 14 477.00 6.35 554 842 859 17 476.95 6.37 555 843 846 3 811.20 1.60 556 843 852 9 477.13 6.30 557 846 851 5 782.62 12.42 558 846 852 6 726.00 2.93 559 846 855 9 634.65 14.98 560 848 851 3 862.44 6.15 561 848 852 4 819.75 2.60 562 849 852 3 819.25 2.95 563 849 853 4 819.16 2.91 564 850 853 3 848.49 0.12 565 850 854 4 819.38 3.02 566 851 854 3 845.34 1.46 567 851 855 4 831.46 0.10 568 852 855 3 846.75 1.00 569 852 856 4 828.56 1.54 570 853 856 3 844.06 0.12 571 853 857 4 834.63 4.58 572 854 857 3 848.27 9.03 573 854 858 4 824.33 0.51 574 855 858 3 859.80 3.27 575 855 859 4 820.00 2.67 576 856 859 3 839.21 5.20 577 856 860 4 820.00 2.67 578 857 860 3 848.33 0.64 579 857 861 4 827.26 6.29 580 857 917 60 293.88 1.94 581 858 861 3 844.72 2.43 582 858 862 4 843.80 1.98 583 859 862 3 843.80 1.98 584 859 863 4 836.57 5.59 585 860 863 3 843.76 2.00 586 860 864 4 819.49 2.94 587 860 916 56 293.89 1.94 588 860 917 57 293.88 1.94 589 861 864 3 855.64 9.37 590 861 865 4 843.46 3.27 591 861 917 56 293.87 1.94 592 862 865 3 857.89 3.94 593 862 866 4 849.06 0.52 594 863 866 3 868.65 2.68 595 863 916 53 293.89 1.94 596 864 867 3 871.28 1.37 597 864 868 4 863.63 5.19 598 864 915 51 293.98 1.90 599 864 916 52 293.89 1.94 600 864 917 53 293.87 1.95 601 865 868 3 876.20 0.03 602 865 869 4 854.94 2.42 603 866 869 3 858.55 4.14 604 866 870 4 837.55 6.36 605 867 870 3 850.15 0.06 606 867 871 4 837.55 6.36 607 867 913 46 295.08 1.62 608 867 915 48 293.98 1.90 609 868 871 3 848.72 0.78 610 868 872 4 840.51 4.88 611 868 915 47 294.35 1.72 612 869 872 3 846.69 0.24 613 869 873 4 833.58 1.42 614 870 873 3 843.68 1.27 615 870 874 4 826.16 1.23 616 871 874 3 845.13 4.22 617 871 875 4 826.16 1.23 618 871 906 35 302.98 1.91 619 871 907 36 302.92 1.94 620 872 875 3 853.05 0.27 621 872 876 4 829.11 6.44 622 873 876 3 840.81 5.85 623 873 877 4 829.12 6.44 624 874 877 3 853.10 1.24 625 874 878 4 829.11 6.45 626 874 903 29 303.02 1.92 627 874 906 32 303.00 1.91 628 874 907 33 302.94 1.94 629 875 878 3 848.49 1.06 630 875 879 4 829.13 6.46 631 875 907 32 302.99 1.91 632 876 879 3 846.34 2.15 633 876 880 4 829.13 6.46 634 877 880 3 839.14 1.45 635 877 881 4 829.12 6.46 636 877 891 14 481.92 6.79 637 877 899 22 303.54 1.97 638 878 881 3 844.46 1.46 639 878 882 4 829.44 6.24 640 878 899 21 303.54 1.97 641 878 903 25 303.37 1.97 642 878 904 26 303.23 1.99 643 878 907 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