BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 25 262 237 21.16 0.56 2 25 263 238 20.66 0.53 3 26 29 3 971.97 0.34 4 26 72 46 360.94 0.56 5 26 109 83 221.06 1.77 6 26 110 84 208.43 1.26 7 26 259 233 21.52 0.69 8 26 262 236 22.98 0.04 9 26 263 237 21.48 0.68 10 26 264 238 24.38 0.78 11 26 267 241 21.48 0.68 12 27 72 45 360.94 0.56 13 27 73 46 360.94 0.56 14 27 109 82 230.29 0.55 15 28 72 44 361.00 0.58 16 28 73 45 360.94 0.56 17 28 107 79 230.93 0.45 18 28 108 80 230.34 0.54 19 28 109 81 230.31 0.55 20 28 110 82 230.30 0.55 21 29 72 43 361.04 0.58 22 29 73 44 361.01 0.58 23 29 74 45 360.96 0.57 24 29 75 46 360.95 0.56 25 29 108 79 230.84 0.48 26 29 110 81 230.31 0.55 27 29 112 83 230.30 0.55 28 29 256 227 73.11 1.88 29 29 259 230 54.42 0.61 30 30 75 45 360.98 0.57 31 30 77 47 360.94 0.56 32 30 102 72 232.04 0.25 33 30 106 76 232.03 0.24 34 30 108 78 230.93 0.45 35 30 110 80 230.69 0.41 36 30 111 81 230.33 0.55 37 30 112 82 230.31 0.55 38 31 42 11 374.05 0.35 39 31 74 43 361.05 0.58 40 31 75 44 361.01 0.58 41 31 76 45 360.95 0.56 42 31 77 46 360.94 0.56 43 31 112 81 230.32 0.55 44 31 114 83 230.29 0.55 45 31 252 221 89.12 0.10 46 31 256 225 75.55 0.73 47 32 77 45 360.98 0.57 48 32 98 66 232.40 0.14 49 32 99 67 232.09 0.28 50 32 111 79 230.94 0.45 51 32 112 80 230.72 0.42 52 32 113 81 230.31 0.55 53 32 114 82 230.34 0.56 54 32 206 174 230.84 0.44 55 33 38 5 833.09 3.10 56 33 39 6 694.36 64.00 57 33 42 9 563.12 0.39 58 33 76 43 361.03 0.58 59 33 77 44 361.01 0.57 60 33 78 45 360.94 0.56 61 33 79 46 360.97 0.57 62 33 114 81 230.37 0.55 63 34 38 4 844.41 2.56 64 34 79 45 360.98 0.58 65 34 88 54 232.40 0.14 66 34 90 56 231.91 0.21 67 34 202 168 230.92 0.46 68 35 38 3 855.72 3.10 69 35 39 4 844.65 2.44 70 35 88 53 232.39 0.14 71 35 90 55 232.19 0.11 72 35 237 202 108.67 1.06 73 35 241 206 104.17 0.67 74 36 39 3 844.65 2.44 75 36 40 4 839.96 0.09 76 36 88 52 232.50 0.20 77 37 40 3 856.19 3.18 78 37 41 4 856.56 3.48 79 38 41 3 893.14 3.21 80 38 115 77 230.34 0.55 81 38 116 78 230.32 0.54 82 38 245 207 103.42 0.62 83 38 248 210 103.31 0.58 84 39 42 3 862.90 0.30 85 39 43 4 843.84 2.86 86 39 76 37 361.14 0.56 87 39 78 39 361.04 0.56 88 40 43 3 847.01 4.19 89 40 44 4 844.11 2.73 90 40 65 25 361.45 0.63 91 41 44 3 868.57 1.95 92 41 45 4 848.00 1.20 93 41 245 204 103.42 0.62 94 41 249 208 103.14 0.58 95 42 45 3 849.65 0.38 96 42 46 4 844.77 2.82 97 42 76 34 361.47 0.62 98 42 249 207 102.66 0.35 99 42 252 210 92.47 0.72 100 43 46 3 866.55 0.75 101 43 47 4 829.36 4.63 102 43 48 5 586.60 96.95 103 43 65 22 361.53 0.66 104 43 76 33 361.47 0.63 105 44 47 3 822.89 1.50 106 45 249 204 103.17 0.59 107 45 253 208 90.05 0.49 108 46 69 23 371.88 0.50 109 46 74 28 371.83 0.54 110 46 253 207 89.26 0.17 111 46 256 210 79.36 0.03 112 48 65 17 371.92 0.49 113 48 66 18 371.93 0.49 114 48 67 19 371.93 0.49 115 48 74 26 371.94 0.50 116 48 76 28 371.87 0.52 117 49 65 16 371.94 0.48 118 49 66 17 371.95 0.49 119 50 63 13 371.96 0.49 120 50 64 14 371.96 0.49 121 50 65 15 371.95 0.49 122 51 55 4 827.09 0.79 123 51 64 13 371.99 0.48 124 51 66 15 372.01 0.47 125 54 57 3 865.37 9.14 126 55 64 9 372.12 0.48 127 56 62 6 372.87 0.85 128 56 101 45 231.54 0.71 129 56 102 46 231.19 0.54 130 56 105 49 231.09 0.49 131 57 60 3 867.49 1.24 132 61 79 18 372.23 0.43 133 61 80 19 372.09 0.39 134 61 81 20 372.06 0.40 135 62 78 16 372.24 0.43 136 62 79 17 372.22 0.43 137 62 97 35 232.89 0.07 138 62 98 36 232.65 0.15 139 62 101 39 232.96 0.11 140 63 77 14 372.39 0.41 141 63 78 15 372.31 0.42 142 63 80 17 372.22 0.42 143 64 75 11 372.80 0.29 144 64 76 12 372.58 0.38 145 64 77 13 372.40 0.42 146 64 106 42 232.52 0.21 147 65 75 10 372.80 0.29 148 65 76 11 372.60 0.38 149 65 78 13 372.60 0.38 150 66 74 8 372.92 0.30 151 66 75 9 372.79 0.29 152 67 73 6 372.99 0.31 153 67 74 7 372.98 0.32 154 68 72 4 730.03 27.00 155 69 72 3 851.24 7.05 156 69 74 5 479.47 51.59 157 69 256 187 74.58 1.24 158 69 257 188 73.35 1.83 159 69 260 191 54.02 0.82 160 70 257 187 74.55 1.24 161 70 260 190 54.02 0.82 162 71 260 189 54.02 0.82 163 72 256 184 73.17 1.91 164 72 259 187 55.77 0.05 165 72 260 188 53.96 0.79 166 74 256 182 73.19 1.90 167 75 106 31 232.00 0.24 168 77 98 21 232.41 0.14 169 77 102 25 232.46 0.16 170 77 106 29 232.62 0.18 171 78 98 20 232.61 0.14 172 79 89 10 232.38 0.14 173 79 94 15 232.41 0.14 174 79 98 19 232.66 0.15 175 80 88 8 232.59 0.17 176 80 89 9 232.56 0.15 177 81 86 5 232.96 0.04 178 81 87 6 232.87 0.08 179 82 85 3 843.18 0.86 180 82 86 4 302.75 34.92 181 82 87 5 232.97 0.04 182 82 89 7 232.56 0.17 183 89 94 5 802.55 6.38 184 90 94 4 843.04 0.26 185 90 201 111 245.00 0.91 186 90 202 112 247.11 0.01 187 91 94 3 874.55 0.76 188 91 95 4 843.35 0.39 189 91 179 88 247.09 0.04 190 91 201 110 245.58 0.64 191 91 202 111 247.12 0.02 192 92 95 3 852.23 0.97 193 92 96 4 843.08 0.25 194 92 138 46 247.33 0.01 195 92 177 85 247.24 0.04 196 92 179 87 247.25 0.03 197 92 201 109 247.16 0.01 198 92 202 110 247.16 0.01 199 92 205 113 247.32 0.02 200 93 96 3 852.31 3.02 201 93 97 4 843.36 0.39 202 94 97 3 843.37 0.39 203 94 98 4 843.07 0.24 204 95 98 3 847.50 1.48 205 95 99 4 838.41 2.07 206 95 202 107 247.55 0.04 207 95 206 111 247.61 0.07 208 96 99 3 847.22 5.86 209 96 100 4 835.56 0.60 210 96 205 109 247.61 0.07 211 96 209 113 247.97 0.07 212 97 100 3 857.55 0.69 213 97 101 4 846.40 6.03 214 98 101 3 851.27 3.60 215 98 102 4 831.39 1.48 216 99 102 3 856.33 1.06 217 99 103 4 841.00 6.53 218 99 111 12 247.99 0.11 219 99 206 107 247.91 0.06 220 99 209 110 249.49 0.45 221 100 103 3 846.31 3.89 222 100 104 4 843.82 5.12 223 100 209 109 248.12 0.07 224 100 213 113 417.45 2.23 225 101 104 3 849.98 2.04 226 101 105 4 843.82 5.12 227 102 105 3 848.41 2.83 228 102 106 4 840.86 6.59 229 102 107 5 826.70 0.49 230 102 108 6 807.97 4.19 231 103 107 4 798.60 8.88 232 103 108 5 798.57 8.88 233 103 111 8 607.95 78.53 234 103 209 106 437.19 6.83 235 103 210 107 437.25 6.83 236 103 213 110 437.26 6.82 237 103 215 112 437.25 6.83 238 104 107 3 860.52 1.72 239 104 213 109 449.84 0.54 240 108 111 3 901.38 0.03 241 108 215 107 543.57 0.76 242 109 216 107 543.56 0.76 243 109 264 155 43.16 0.73 244 110 159 49 543.56 0.76 245 110 215 105 543.60 0.76 246 110 216 106 543.57 0.76 247 110 218 108 543.55 0.76 248 110 264 154 43.66 0.48 249 110 267 157 42.10 0.23 250 111 206 95 543.75 0.73 251 111 210 99 543.90 0.80 252 111 215 104 543.76 0.76 253 111 216 105 543.61 0.77 254 111 217 106 543.57 0.76 255 111 218 107 543.56 0.76 256 112 218 106 543.56 0.76 257 112 220 108 543.56 0.76 258 112 255 143 75.89 0.58 259 112 256 144 75.63 0.70 260 112 259 147 70.59 1.48 261 113 203 90 543.61 0.76 262 113 206 93 543.99 0.80 263 113 218 105 543.63 0.76 264 113 219 106 543.56 0.76 265 113 220 107 543.56 0.76 266 113 221 108 543.56 0.76 267 114 220 106 543.56 0.76 268 114 248 134 103.30 0.59 269 115 220 105 543.64 0.76 270 115 221 106 543.57 0.76 271 115 222 107 543.54 0.76 272 115 248 133 103.30 0.59 273 116 222 106 543.55 0.77 274 116 224 108 543.13 0.65 275 116 237 121 108.66 1.05 276 116 241 125 104.38 0.77 277 116 244 128 104.05 0.65 278 116 245 129 103.73 0.78 279 117 198 81 544.04 0.80 280 117 199 82 543.99 0.83 281 117 200 83 544.00 0.80 282 117 221 104 543.77 0.75 283 117 222 105 543.60 0.76 284 117 223 106 543.54 0.77 285 117 224 107 543.51 0.78 286 118 198 80 544.05 0.80 287 118 199 81 544.02 0.81 288 118 225 107 543.48 0.77 289 118 227 109 499.74 0.30 290 119 125 6 543.63 0.73 291 119 197 78 544.05 0.80 292 119 198 79 544.05 0.80 293 119 223 104 543.75 0.74 294 119 225 106 543.56 0.77 295 119 226 107 517.74 1.49 296 119 227 108 505.15 1.28 297 120 123 3 858.83 3.48 298 120 124 4 824.40 2.35 299 120 125 5 657.75 57.66 300 120 183 63 544.52 0.84 301 120 191 71 544.15 0.87 302 120 197 77 544.05 0.80 303 120 199 79 544.37 0.83 304 120 227 107 526.38 2.54 305 120 228 108 503.98 1.82 306 121 124 3 849.74 8.03 307 121 125 4 795.26 5.73 308 121 226 105 543.58 0.77 309 121 228 107 517.71 6.15 310 122 228 106 542.44 0.27 311 123 195 72 544.28 0.83 312 123 196 73 544.33 0.82 313 123 197 74 544.32 0.82 314 125 129 4 837.76 4.04 315 125 131 6 546.66 0.29 316 125 197 72 544.47 0.82 317 125 223 98 544.33 0.80 318 125 226 101 543.72 0.76 319 126 129 3 854.62 4.65 320 126 223 97 544.41 0.78 321 126 226 100 543.87 0.81 322 127 223 96 544.37 0.81 323 127 224 97 544.33 0.81 324 127 225 98 544.25 0.78 325 127 226 99 544.19 0.79 326 127 236 109 108.85 1.01 327 127 240 113 108.31 0.89 328 127 244 117 104.62 0.67 329 128 223 95 544.45 0.79 330 128 244 116 104.14 0.62 331 129 171 42 759.40 0.73 332 129 222 93 544.62 0.72 333 129 223 94 544.46 0.79 334 130 170 40 763.01 2.00 335 130 220 90 545.70 0.67 336 130 221 91 545.46 0.57 337 130 222 92 544.62 0.72 338 130 247 117 103.50 0.56 339 130 248 118 103.33 0.59 340 130 251 121 103.23 0.64 341 131 171 40 763.02 2.00 342 131 172 41 762.89 1.95 343 131 173 42 761.54 1.31 344 131 197 66 659.37 1.00 345 131 198 67 612.56 2.54 346 131 219 88 548.10 0.72 347 131 220 89 546.89 0.91 348 131 221 90 545.66 0.66 349 131 223 92 545.48 0.57 350 132 161 29 763.39 2.00 351 132 164 32 763.30 2.04 352 132 165 33 763.21 2.02 353 132 170 38 763.16 2.02 354 132 171 39 763.09 2.02 355 132 172 40 762.99 1.99 356 132 173 41 763.02 2.00 357 132 218 86 548.12 0.73 358 132 219 87 548.10 0.72 359 132 220 88 547.78 0.69 360 133 158 25 763.58 2.00 361 133 172 39 763.08 2.00 362 133 173 40 763.05 2.01 363 133 175 42 746.64 1.92 364 133 198 65 658.39 0.47 365 133 219 86 548.10 0.72 366 133 221 88 547.87 0.74 367 134 157 23 763.70 2.00 368 134 158 24 763.66 2.01 369 134 172 38 763.16 2.02 370 134 173 39 763.09 2.00 371 134 174 40 763.05 2.01 372 135 155 20 763.84 2.03 373 135 156 21 763.75 2.02 374 135 157 22 763.70 2.00 375 135 158 23 763.69 2.00 376 135 174 39 763.08 2.00 377 135 175 40 763.08 2.00 378 136 154 18 763.97 2.02 379 136 155 19 763.85 2.03 380 136 156 20 763.83 2.04 381 136 175 39 763.10 2.01 382 136 177 41 763.03 2.00 383 136 203 67 598.21 4.34 384 136 204 68 574.41 7.21 385 136 207 71 556.84 1.54 386 136 219 83 548.15 0.72 387 137 153 16 763.97 2.02 388 137 155 18 763.85 2.03 389 137 174 37 763.22 2.03 390 137 175 38 763.16 2.04 391 137 176 39 763.06 2.00 392 137 177 40 763.04 2.00 393 137 194 57 660.97 1.09 394 137 204 67 598.15 4.34 395 138 141 3 885.95 5.07 396 138 152 14 764.08 2.00 397 138 153 15 764.06 1.99 398 138 177 39 763.06 2.00 399 138 204 66 607.27 0.17 400 138 205 67 607.27 0.18 401 139 176 37 763.20 2.03 402 139 177 38 763.16 2.03 403 139 178 39 763.12 2.02 404 139 187 48 661.14 1.08 405 139 190 51 661.10 1.08 406 139 191 52 661.09 1.08 407 139 194 55 661.09 1.08 408 139 199 60 660.69 1.03 409 140 178 38 763.16 2.03 410 140 179 39 763.16 2.03 411 140 187 47 661.15 1.09 412 141 151 10 764.22 2.00 413 141 152 11 764.20 2.00 414 141 154 13 764.19 2.00 415 142 186 44 661.27 1.08 416 142 190 48 661.13 1.10 417 143 148 5 764.38 2.02 418 143 149 6 764.35 2.04 419 143 151 8 764.38 2.02 420 143 152 9 764.27 2.01 421 145 148 3 855.66 2.09 422 145 149 4 786.97 8.07 423 147 150 3 852.35 1.40 424 147 151 4 828.01 4.30 425 148 151 3 852.35 1.40 426 148 152 4 838.02 0.70 427 149 152 3 854.15 2.31 428 149 153 4 838.04 0.69 429 150 153 3 837.11 5.10 430 150 154 4 828.12 4.27 431 151 154 3 844.13 0.01 432 155 174 19 763.25 2.04 433 156 207 51 556.83 1.54 434 156 208 52 556.81 1.54 435 156 211 55 553.32 0.71 436 158 217 59 548.51 0.80 437 158 219 61 548.33 0.80 438 159 216 57 548.54 0.79 439 159 217 58 548.54 0.79 440 159 218 59 548.49 0.80 441 159 264 105 48.86 1.08 442 159 267 108 43.72 0.45 443 161 164 3 853.83 4.83 444 161 165 4 826.31 3.69 445 161 218 57 548.49 0.79 446 161 267 106 43.13 0.74 447 162 165 3 849.56 6.43 448 162 166 4 836.63 1.50 449 162 267 105 46.02 0.17 450 163 166 3 840.52 1.92 451 163 167 4 836.63 1.50 452 163 258 95 80.75 0.71 453 163 266 103 49.31 1.23 454 163 267 104 45.12 0.05 455 164 167 3 845.13 2.13 456 164 168 4 813.90 1.41 457 164 170 6 807.65 4.17 458 164 255 91 88.93 0.21 459 164 258 94 77.22 0.64 460 164 267 103 43.06 0.15 461 165 169 4 764.48 1.88 462 165 170 5 764.48 1.88 463 165 172 7 764.41 1.87 464 167 258 91 85.96 1.11 465 168 251 83 102.62 0.44 466 168 254 86 95.07 1.20 467 168 255 87 93.91 0.70 468 168 258 90 86.47 1.03 469 170 220 50 548.09 0.72 470 170 251 81 103.03 0.59 471 173 197 24 660.44 1.08 472 174 194 20 660.76 1.04 473 174 196 22 660.56 1.06 474 174 197 23 660.49 1.09 475 174 198 24 660.45 1.08 476 175 198 23 660.46 1.08 477 175 199 24 660.45 1.08 478 175 200 25 660.45 1.08 479 175 203 28 598.18 4.33 480 175 219 44 548.10 0.72 481 175 221 46 545.44 0.56 482 176 191 15 660.80 1.06 483 176 194 18 661.02 1.10 484 176 196 20 660.71 1.03 485 176 198 22 660.62 1.04 486 176 199 23 660.47 1.09 487 176 200 24 660.45 1.08 488 177 200 23 660.45 1.08 489 177 201 24 660.30 1.09 490 177 202 25 660.21 1.12 491 177 203 26 629.29 2.58 492 177 204 27 607.25 0.18 493 178 200 22 660.63 1.03 494 178 201 23 660.44 1.09 495 179 201 22 660.61 1.04 496 180 199 19 661.05 1.08 497 180 201 21 660.69 1.05 498 181 199 18 661.04 1.08 499 181 201 20 660.74 1.03 500 181 233 52 109.23 1.28 501 181 234 53 109.23 1.28 502 181 237 56 109.17 1.30 503 182 199 17 661.04 1.08 504 183 187 4 827.37 4.46 505 183 188 5 808.93 1.88 506 183 191 8 804.30 0.24 507 183 199 16 661.03 1.08 508 183 227 44 503.97 1.83 509 184 187 3 845.84 1.83 510 184 188 4 842.94 3.29 511 185 188 3 852.96 1.72 512 185 189 4 844.12 2.72 513 186 189 3 859.00 1.30 514 186 190 4 844.12 2.72 515 187 190 3 859.53 0.97 516 187 191 4 844.12 2.72 517 188 191 3 844.13 2.71 518 188 192 4 843.00 3.27 519 189 192 3 850.31 3.64 520 189 193 4 829.93 3.27 521 190 193 3 832.85 1.83 522 190 194 4 828.11 4.17 523 191 194 3 863.49 10.23 524 191 195 4 828.13 4.16 525 191 196 5 818.88 7.81 526 191 199 8 660.97 1.04 527 192 195 3 839.34 2.33 528 198 221 23 544.61 0.70 529 198 223 25 544.25 0.58 530 199 227 28 503.99 1.82 531 200 203 3 894.14 2.04 532 200 221 21 544.94 0.40 533 202 205 3 861.56 0.62 534 202 206 4 832.21 0.70 535 203 206 3 853.87 4.48 536 203 207 4 837.38 3.28 537 203 219 16 548.12 0.71 538 204 207 3 837.38 3.27 539 204 208 4 837.38 3.27 540 205 208 3 844.96 0.51 541 205 209 4 832.12 1.42 542 206 209 3 845.31 0.34 543 206 210 4 829.49 0.10 544 206 217 11 548.51 0.79 545 207 210 3 850.27 4.88 546 207 211 4 815.62 5.75 547 207 217 10 548.51 0.79 548 207 219 12 548.40 0.74 549 208 211 3 855.52 2.25 550 208 212 4 828.37 1.28 551 209 212 3 847.06 6.49 552 209 213 4 828.28 1.23 553 210 213 3 844.58 4.39 554 210 214 4 808.07 2.89 555 210 215 5 663.17 56.52 556 210 217 7 548.53 0.79 557 211 214 3 813.10 0.88 558 216 264 48 46.32 0.32 559 220 248 28 103.31 0.59 560 220 251 31 100.62 0.32 561 220 252 32 93.80 0.64 562 220 255 35 87.95 0.79 563 222 244 22 104.05 0.65 564 222 248 26 103.32 0.59 565 223 226 3 935.15 5.75 566 223 244 21 104.09 0.64 567 224 236 12 108.74 1.06 568 224 237 13 108.67 1.05 569 224 240 16 104.65 0.70 570 224 241 17 104.65 0.70 571 224 244 20 104.36 0.78 572 225 233 8 109.23 1.20 573 225 236 11 108.78 1.04 574 225 237 12 108.70 1.06 575 226 229 3 819.10 7.14 576 226 236 10 109.24 1.19 577 231 236 5 804.69 2.08 578 232 235 3 844.70 1.79 579 232 236 4 823.76 4.90 580 233 236 3 842.88 3.98 581 233 237 4 829.93 0.18 582 234 237 3 843.08 0.73 583 234 238 4 832.73 1.21 584 235 238 3 871.57 1.11 585 235 239 4 831.77 1.10 586 236 239 3 859.57 7.11 587 236 240 4 831.78 1.10 588 237 240 3 846.88 0.07 589 237 241 4 830.35 0.39 590 238 241 3 832.47 0.67 591 240 243 3 851.48 4.36 592 240 244 4 830.93 0.45 593 241 244 3 844.93 0.84 594 241 245 4 837.12 3.44 595 242 245 3 854.53 5.27 596 242 246 4 837.14 3.43 597 243 246 3 856.57 4.25 598 243 247 4 839.34 2.37 599 244 247 3 843.18 0.45 600 244 248 4 837.18 3.45 601 245 248 3 839.34 2.37 602 245 249 4 839.69 2.19 603 246 249 3 844.55 0.24 604 246 250 4 839.90 2.08 605 247 250 3 848.09 2.02 606 247 251 4 839.66 2.19 607 248 251 3 843.93 4.64 608 248 252 4 829.68 0.34 609 249 252 3 839.05 3.81 610 249 253 4 829.66 0.35 611 250 253 3 847.87 0.61 612 250 254 4 839.19 2.95 613 251 254 3 847.68 1.29 614 251 255 4 836.51 4.28 615 252 255 3 848.81 1.85 616 252 256 4 827.17 0.39 617 253 256 3 843.03 3.62 618 253 257 4 829.86 1.72 619 254 257 3 849.51 7.04 620 254 258 4 827.15 0.35 621 255 258 3 832.13 0.43 622 255 259 4 829.15 2.29 623 256 259 3 837.19 3.07 624 256 260 4 830.36 1.69 625 257 260 3 835.98 1.11 626 258 262 4 836.62 1.43 627 258 267 9 113.12 0.41 628 259 262 3 836.52 1.48 629 259 267 8 76.42 17.30 630 262 266 4 710.76 58.65 631 262 267 5 699.20 52.87 632 263 266 3 850.44 0.12 633 263 267 4 835.76 0.18 634 264 267 3 858.43 4.12