BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 8 127 120 48.74 0.60 2 8 128 121 30.10 0.96 3 9 127 119 48.88 0.54 4 10 127 118 50.66 0.83 5 10 128 119 50.66 0.83 6 11 14 3 864.96 3.77 7 11 125 115 50.72 0.83 8 11 126 116 50.88 0.91 9 11 127 117 50.87 0.91 10 12 23 11 50.89 0.92 11 12 128 117 50.88 0.92 12 12 129 118 50.87 0.91 13 13 21 8 51.00 0.96 14 13 23 10 50.89 0.92 15 13 24 11 50.90 0.91 16 13 124 112 50.92 0.92 17 13 131 119 50.90 0.91 18 14 21 7 251.10 99.09 19 15 20 5 622.89 2.52 20 15 21 6 251.11 99.08 21 16 19 3 789.67 6.44 22 16 20 4 652.31 13.02 23 20 25 5 792.99 10.07 24 21 24 3 834.72 0.14 25 21 25 4 823.79 5.32 26 22 25 3 827.33 3.56 27 22 26 4 823.81 5.32 28 23 26 3 823.81 5.32 29 23 27 4 808.60 2.31 30 23 131 109 369.04 1.32 31 24 27 3 831.12 8.95 32 24 28 4 808.59 2.31 33 24 124 101 369.03 1.33 34 24 131 108 369.53 1.10 35 24 154 132 367.44 1.59 36 25 28 3 827.94 2.92 37 25 29 4 808.55 2.35 38 26 29 3 824.84 5.79 39 26 30 4 814.92 0.83 40 27 30 3 824.84 5.79 41 27 31 4 812.74 0.26 42 27 131 105 371.02 0.39 43 27 132 106 371.03 0.38 44 27 133 107 371.00 0.38 45 27 153 128 370.93 0.37 46 28 31 3 816.33 1.54 47 28 32 4 812.74 0.26 48 28 153 127 370.97 0.36 49 29 32 3 820.34 0.47 50 29 33 4 811.87 0.20 51 30 33 3 829.72 1.07 52 30 34 4 808.82 1.72 53 31 34 3 814.44 1.05 54 31 79 48 371.35 0.42 55 31 80 49 371.37 0.42 56 31 133 103 371.37 0.42 57 31 134 104 371.34 0.42 58 31 135 105 371.30 0.42 59 31 153 124 371.34 0.42 60 31 166 137 350.06 1.42 61 32 166 136 350.05 1.48 62 32 167 137 349.99 1.46 63 32 169 139 346.24 1.37 64 32 170 140 345.24 1.17 65 33 167 136 350.03 1.47 66 34 80 46 371.74 0.53 67 34 166 134 350.33 1.36 68 34 167 135 350.12 1.44 69 35 50 15 427.89 5.89 70 35 51 16 427.86 5.89 71 35 53 18 427.85 5.88 72 35 66 31 427.62 5.83 73 35 79 44 398.16 9.76 74 36 50 14 427.89 5.89 75 36 51 15 427.90 5.89 76 36 164 130 350.75 1.31 77 36 165 131 350.61 1.32 78 36 166 132 350.53 1.32 79 36 167 133 350.50 1.33 80 36 168 134 350.41 1.37 81 36 197 163 262.04 0.06 82 36 200 166 261.50 0.10 83 37 49 12 427.93 5.90 84 37 50 13 427.89 5.89 85 37 77 40 426.15 5.17 86 37 78 41 425.29 4.99 87 37 79 42 422.18 6.54 88 37 137 101 425.55 4.87 89 37 164 129 350.70 1.30 90 37 165 130 350.63 1.31 91 38 48 10 427.94 5.90 92 38 49 11 427.94 5.90 93 38 137 100 427.36 5.73 94 38 163 127 350.94 1.37 95 38 164 128 350.73 1.31 96 38 193 157 263.05 0.01 97 38 196 160 263.02 0.01 98 38 197 161 262.99 0.00 99 39 47 8 428.05 5.87 100 39 48 9 427.93 5.90 101 39 137 99 427.68 5.83 102 39 148 111 427.77 5.82 103 39 162 125 368.55 0.58 104 39 163 126 352.43 0.88 105 40 46 6 430.01 6.83 106 40 47 7 429.98 6.82 107 40 148 110 427.88 5.85 108 40 161 123 370.88 0.41 109 40 162 124 368.56 0.58 110 40 176 138 336.18 0.49 111 40 180 142 314.00 0.63 112 40 189 151 263.47 0.14 113 40 192 154 263.42 0.12 114 40 193 155 263.33 0.14 115 41 45 4 742.06 2.42 116 41 46 5 446.44 1.39 117 41 147 107 428.10 5.87 118 41 14 108 428.08 5.87 119 41 160 121 372.11 0.51 120 41 161 122 371.18 0.61 121 41 180 141 324.24 2.20 122 42 45 3 804.81 3.77 123 42 184 144 275.05 2.37 124 42 185 145 267.88 0.76 125 42 186 146 266.64 0.29 126 42 189 149 265.11 0.82 127 43 182 141 308.08 1.20 128 43 183 142 290.36 1.70 129 43 184 143 285.88 0.71 130 44 147 104 443.79 1.03 131 44 185 143 275.89 1.95 132 44 186 144 268.44 0.98 133 44 216 174 261.17 0.01 134 45 147 103 443.83 1.05 135 45 186 143 267.61 0.84 136 45 215 172 261.13 0.01 137 45 216 173 260.66 0.18 138 46 139 94 443.82 1.05 139 46 147 102 444.05 1.11 140 46 14 103 443.85 1.05 141 46 148 104 443.81 1.04 142 46 186 142 267.52 0.79 143 46 214 170 261.13 0.01 144 46 215 171 260.69 0.18 145 47 189 144 263.69 0.10 146 47 190 145 263.63 0.11 147 47 193 148 263.43 0.12 148 47 212 167 261.30 0.01 149 47 213 168 261.17 0.01 150 47 214 169 260.66 0.18 151 48 67 19 444.19 0.99 152 48 75 27 444.28 1.02 153 48 76 28 444.25 1.03 154 48 77 29 444.10 0.98 155 48 137 90 443.83 1.06 156 48 139 92 444.24 1.04 157 48 148 102 444.24 1.04 158 48 212 166 261.16 0.00 159 48 213 167 261.08 0.00 160 49 197 150 263.02 0.01 161 49 211 164 261.21 0.01 162 49 212 165 260.67 0.18 163 50 66 16 444.41 1.02 164 50 67 17 444.42 1.03 165 50 77 27 444.28 1.02 166 50 197 149 262.95 0.01 167 50 210 162 261.24 0.03 168 50 211 163 261.14 0.03 169 51 66 15 444.54 1.01 170 51 202 152 261.74 0.01 171 51 20 160 261.33 0.04 172 51 210 161 261.28 0.06 173 52 61 9 444.94 1.01 174 52 62 10 444.95 1.01 175 52 66 14 444.50 0.99 176 52 209 158 261.37 0.04 177 52 20 159 261.30 0.06 178 52 210 160 261.28 0.06 179 52 211 161 261.20 0.03 180 54 59 5 621.39 2.88 181 56 59 3 809.31 3.63 182 60 63 3 824.00 3.73 183 61 64 3 829.93 4.32 184 65 211 148 261.17 0.01 185 65 223 175 133.49 0.50 186 66 77 11 514.90 4.28 187 66 78 12 514.88 4.27 188 66 85 19 514.56 4.47 189 66 211 147 261.12 0.01 190 67 76 9 576.74 18.70 191 67 77 10 520.39 1.55 192 67 85 18 535.78 1.54 193 67 211 146 261.19 0.01 194 67 213 148 260.68 0.19 195 67 221 171 150.95 1.23 196 67 223 173 121.61 0.03 197 68 75 7 585.00 14.95 198 68 76 8 584.89 14.93 199 68 85 17 543.65 2.24 200 68 89 21 550.15 2.44 201 68 93 25 580.84 11.62 202 68 221 170 150.95 1.23 203 69 72 3 863.11 0.46 204 69 74 5 638.93 9.68 205 69 75 6 617.97 1.54 206 69 93 24 580.92 11.64 207 69 221 169 150.31 0.90 208 70 74 4 730.06 4.75 209 70 75 5 674.36 29.55 210 70 76 6 618.15 1.45 211 70 89 19 556.43 0.57 212 70 92 22 556.48 0.56 213 70 93 23 580.74 11.57 214 70 112 43 614.27 1.56 215 70 138 69 556.47 0.56 216 71 74 3 817.27 7.00 217 71 96 25 614.31 1.58 218 71 106 36 614.91 1.40 219 71 107 37 614.97 1.43 220 71 112 42 614.47 1.56 221 72 106 35 615.38 1.39 222 72 219 164 150.44 0.85 223 72 220 165 151.38 1.28 224 72 221 166 151.34 1.27 225 73 140 68 604.62 1.45 226 73 141 69 604.54 1.42 227 73 215 144 261.13 0.03 228 73 219 163 151.33 1.27 229 73 220 164 151.35 1.27 230 73 221 165 151.33 1.27 231 74 107 34 615.49 1.43 232 74 109 36 615.30 1.34 233 74 112 39 615.30 1.34 234 74 138 65 605.00 1.31 235 74 139 66 604.96 1.30 236 74 140 67 604.62 1.45 237 74 215 143 261.07 0.00 238 74 221 164 151.01 1.25 239 75 138 64 605.00 1.31 240 75 139 65 604.97 1.30 241 75 215 142 260.66 0.18 242 75 221 163 150.28 0.89 243 76 85 9 615.70 1.31 244 76 137 62 605.20 1.33 245 76 138 63 605.00 1.31 246 77 85 8 616.03 1.17 247 77 136 60 605.77 1.13 248 77 137 61 605.27 1.30 249 78 81 3 1030.00 0.00 250 78 82 4 851.95 0.88 251 78 85 7 617.24 0.57 252 78 134 57 609.68 0.51 253 78 135 58 606.53 0.84 254 78 136 59 605.76 1.12 255 78 165 89 350.53 1.31 256 79 134 56 612.57 0.67 257 79 135 57 608.15 1.21 258 79 165 88 350.51 1.31 259 79 166 89 350.06 1.42 260 80 133 54 614.62 1.63 261 80 134 55 614.26 1.51 262 81 84 3 833.62 2.52 263 81 85 4 818.85 0.38 264 81 130 50 615.20 1.60 265 81 133 53 614.65 1.64 266 81 134 54 614.62 1.62 267 82 85 3 819.00 0.30 268 82 86 4 818.85 0.38 269 83 86 3 819.01 0.30 270 83 87 4 819.00 0.29 271 84 87 3 844.25 1.47 272 84 88 4 819.12 0.23 273 84 130 47 615.27 1.63 274 84 132 49 614.77 1.64 275 84 133 50 614.74 1.66 276 84 134 51 614.62 1.63 277 85 88 3 817.52 2.71 278 85 89 4 815.32 1.67 279 85 134 50 614.09 1.44 280 85 136 52 607.07 0.71 281 86 89 3 815.33 1.67 282 86 90 4 812.22 3.23 283 87 90 3 817.99 2.06 284 87 91 4 812.20 3.23 285 87 132 46 614.95 1.68 286 88 91 3 822.34 0.11 287 88 92 4 811.12 3.73 288 88 116 29 616.33 1.52 289 88 119 32 616.32 1.53 290 88 120 33 616.29 1.54 291 88 132 45 614.87 1.69 292 88 134 47 614.37 1.58 293 88 136 49 606.18 1.28 294 89 92 3 817.00 0.80 295 89 93 4 811.11 3.74 296 90 93 3 817.84 0.37 297 90 94 4 815.13 1.62 298 91 94 3 828.44 3.00 299 91 95 4 815.13 1.63 300 91 119 29 616.36 1.52 301 92 95 3 832.22 3.44 302 92 96 4 815.66 1.36 303 92 112 21 616.50 1.50 304 92 116 25 616.42 1.53 305 93 96 3 836.47 0.61 306 93 97 4 820.39 0.01 307 94 97 3 827.80 3.71 308 94 98 4 816.30 2.02 309 95 98 3 829.55 4.60 310 95 99 4 804.21 8.06 311 95 112 18 616.52 1.51 312 95 115 21 616.44 1.52 313 96 100 4 796.12 12.11 314 96 101 5 745.81 6.08 315 96 107 12 618.69 1.85 316 96 112 17 616.49 1.50 317 97 100 3 829.55 4.61 318 97 101 4 717.02 8.31 319 97 102 5 714.73 9.46 320 97 106 10 620.15 1.99 321 98 102 4 757.85 11.61 322 99 10 4 793.41 4.16 323 100 10 3 812.91 1.30 324 100 103 4 764.95 8.06 325 100 105 6 654.62 3.00 326 100 106 7 620.22 1.99 327 100 107 8 620.18 2.00 328 100 108 9 620.21 1.99 329 100 111 12 619.83 2.06 330 101 104 4 648.26 0.18 331 101 105 5 633.55 4.67 332 101 106 6 620.20 1.98 333 107 111 4 813.50 3.53 334 107 112 5 768.28 5.41 335 108 111 3 836.46 0.46 336 108 112 4 813.50 3.53 337 108 142 34 604.75 1.30 338 108 145 37 604.75 1.29 339 109 112 3 829.72 2.25 340 109 113 4 808.75 1.16 341 109 138 29 605.15 1.31 342 109 140 31 604.98 1.30 343 109 141 32 604.97 1.30 344 109 142 33 604.95 1.30 345 109 14 39 604.69 1.30 346 109 149 41 604.62 1.31 347 110 113 3 819.94 0.31 348 110 114 4 809.45 1.51 349 110 145 35 604.84 1.28 350 110 149 40 604.62 1.31 351 111 114 3 818.16 0.06 352 111 115 4 809.45 1.51 353 112 115 3 809.74 1.36 354 112 116 4 815.76 1.65 355 113 116 3 816.91 1.07 356 113 117 4 815.76 1.67 357 113 138 25 605.34 1.25 358 113 149 37 604.66 1.30 359 113 151 39 604.33 1.39 360 113 157 45 485.68 3.71 361 114 117 3 816.99 1.06 362 114 118 4 815.76 1.67 363 114 157 44 513.46 4.21 364 115 118 3 822.58 1.34 365 115 119 4 819.51 0.21 366 116 119 3 826.39 0.03 367 116 120 4 819.52 0.22 368 116 136 20 605.93 1.15 369 116 138 22 605.77 1.11 370 117 120 3 819.01 0.47 371 117 121 4 813.60 3.18 372 117 151 35 604.61 1.32 373 117 155 39 533.73 2.44 374 117 157 41 513.48 4.22 375 118 121 3 820.75 0.40 376 118 122 4 818.89 0.53 377 119 122 3 830.20 2.04 378 119 123 4 827.11 3.58 379 119 132 13 615.08 1.75 380 120 123 3 827.10 3.58 381 120 124 4 819.09 0.43 382 120 132 12 614.99 1.71 383 120 134 14 614.82 1.65 384 120 151 32 604.67 1.32 385 120 152 33 604.61 1.33 386 120 153 34 604.21 1.45 387 120 155 36 558.49 3.30 388 121 124 3 823.28 2.58 389 121 125 4 817.99 0.07 390 121 154 34 603.69 1.27 391 121 155 35 592.59 1.43 392 122 125 3 819.45 0.60 393 122 126 4 804.89 6.46 394 123 129 6 617.48 2.71 395 123 130 7 615.37 1.67 396 123 131 8 615.33 1.67 397 123 132 9 615.31 1.66 398 124 131 7 615.34 1.67 399 126 129 3 861.76 3.25 400 130 133 3 1030.00 0.00 401 132 153 22 604.43 1.35 402 134 153 20 604.31 1.39 403 135 151 17 604.47 1.38 404 135 152 18 604.30 1.39 405 135 153 19 604.20 1.43 406 135 165 31 350.52 1.32 407 136 150 15 604.49 1.38 408 136 151 16 604.32 1.38 409 136 165 30 350.53 1.31 410 137 148 12 604.65 1.30 411 137 149 13 604.49 1.37 412 137 150 14 604.33 1.39 413 137 163 27 350.78 1.29 414 137 165 29 350.56 1.31 415 138 148 11 604.49 1.38 416 138 149 12 604.48 1.37 417 138 151 14 604.32 1.38 418 139 14 9 604.52 1.36 419 139 148 10 604.44 1.35 420 139 215 77 260.66 0.18 421 140 147 7 604.65 1.30 422 140 14 8 604.62 1.29 423 140 215 76 261.06 0.01 424 141 146 5 604.66 1.30 425 141 147 6 604.64 1.29 426 141 215 75 261.17 0.02 427 141 220 95 151.43 1.29 428 142 145 3 813.02 1.13 429 142 146 4 659.72 10.94 430 147 215 69 261.16 0.01 431 147 216 70 261.11 0.01 432 147 220 89 151.46 1.30 433 148 159 11 466.56 2.15 434 148 160 12 416.48 22.69 435 148 162 14 416.44 22.68 436 149 157 8 485.06 3.94 437 149 158 9 478.08 7.34 438 149 159 10 416.89 22.68 439 150 157 7 485.10 3.96 440 150 158 8 484.48 4.17 441 150 160 10 467.78 2.75 442 150 165 15 350.54 1.31 443 151 156 5 567.40 31.21 444 151 157 6 485.12 3.97 445 152 155 3 809.31 5.54 446 152 156 4 595.31 33.23 447 152 158 6 595.31 33.23 448 160 163 3 861.75 0.83 449 161 180 19 308.22 1.22 450 162 176 14 336.13 0.49 451 162 180 18 311.69 0.53 452 163 173 10 337.18 0.69 453 163 176 13 336.13 0.49 454 164 169 5 349.52 1.39 455 164 172 8 339.20 1.65 456 164 173 9 337.19 0.69 457 164 176 12 336.13 0.50 458 165 169 4 569.64 46.35 459 166 169 3 805.71 2.23 460 168 171 3 825.53 2.16 461 168 172 4 814.57 2.17 462 169 172 3 814.58 2.17 463 169 173 4 814.57 2.17 464 170 173 3 823.19 4.91 465 170 174 4 814.60 2.16 466 171 174 3 815.55 1.69 467 171 175 4 814.60 2.16 468 172 175 3 814.57 2.13 469 172 176 4 814.54 2.14 470 172 196 24 262.25 0.03 471 173 176 3 820.27 2.53 472 173 177 4 815.23 2.46 473 174 177 3 824.89 0.21 474 174 178 4 815.25 2.46 475 175 178 3 824.56 0.23 476 175 179 4 822.60 1.21 477 175 195 20 262.95 0.01 478 175 196 21 262.62 0.15 479 176 179 3 822.60 1.21 480 176 180 4 805.20 2.33 481 176 192 16 263.04 0.01 482 176 196 20 262.91 0.00 483 179 192 13 263.06 0.02 484 179 195 16 263.02 0.02 485 180 184 4 822.38 1.01 486 181 184 3 848.18 2.21 487 184 188 4 787.72 3.65 488 184 192 8 765.56 13.99 489 185 188 3 816.95 3.94 490 185 189 4 816.93 3.93 491 186 189 3 816.95 3.93 492 186 190 4 816.93 3.93 493 186 214 28 261.33 0.03 494 186 216 30 261.10 0.04 495 187 190 3 816.94 3.93 496 187 191 4 816.93 3.94 497 187 232 60 90.34 0.42 498 188 191 3 816.94 3.93 499 188 192 4 813.56 5.62 500 189 192 3 822.18 1.30 501 189 193 4 808.81 3.24 502 190 193 3 815.36 0.04 503 190 194 4 808.80 3.25 504 190 212 22 261.36 0.03 505 190 214 24 261.15 0.02 506 190 224 49 137.23 3.08 507 190 232 57 86.72 1.40 508 191 194 3 816.13 0.12 509 191 195 4 810.41 2.73 510 191 235 59 81.65 0.81 511 191 236 60 47.08 8.86 512 192 195 3 821.51 1.43 513 192 196 4 812.26 1.84 514 193 196 3 821.69 1.52 515 193 197 4 821.01 2.52 516 194 197 3 821.05 2.52 517 194 198 4 821.02 2.53 518 194 206 11 261.57 0.05 519 194 210 16 261.37 0.03 520 194 212 18 261.16 0.01 521 194 229 50 91.86 0.96 522 194 232 53 85.08 0.58 523 194 233 54 84.50 0.41 524 194 236 57 43.90 7.29 525 195 198 3 826.62 0.27 526 195 199 4 825.41 0.34 527 195 236 56 43.61 7.18 528 196 199 3 834.63 1.03 529 197 200 3 826.56 0.26 530 197 202 4 820.95 2.54 531 197 206 8 261.36 0.02 532 197 210 13 261.31 0.04 533 198 202 3 821.48 2.81 534 198 206 7 261.25 0.01 535 198 236 53 43.62 7.18 536 202 205 3 915.80 1.07 537 202 206 4 806.29 4.91 538 202 210 9 261.29 0.07 539 205 20 5 638.71 4.11 540 206 20 4 754.95 2.56 541 206 210 5 647.68 0.38 542 206 233 43 85.25 0.48 543 207 20 3 830.62 3.06 544 207 210 4 742.17 14.81 545 207 230 39 91.03 0.55 546 207 233 42 85.25 0.48 547 208 226 34 93.20 1.22 548 208 229 37 91.46 0.35 549 208 230 38 91.05 0.55 550 208 233 41 86.53 0.04 551 209 226 33 93.60 1.03 552 210 229 34 91.83 0.95 553 211 223 27 121.96 0.20 554 212 223 26 121.97 0.20 555 212 224 27 120.87 0.41 556 212 229 32 92.61 0.58 557 213 221 23 150.28 0.89 558 213 222 24 147.05 0.21 559 213 223 25 121.69 0.06 560 213 224 26 121.62 0.04 561 214 220 21 151.34 1.27 562 214 221 22 150.24 0.91 563 214 222 23 149.90 0.77 564 214 224 25 139.73 1.83 565 215 220 20 151.33 1.26 566 215 221 21 150.24 0.91 567 216 220 19 151.37 1.26 568 217 220 18 151.47 1.29 569 21 21 14 151.84 1.35 570 21 218 15 151.83 1.34 571 21 219 16 151.74 1.33 572 21 220 17 151.47 1.30 573 21 21 3 812.98 5.61 574 21 21 4 796.86 0.49 575 21 21 13 152.09 1.42 576 21 218 14 151.88 1.32 577 21 219 15 151.87 1.33 578 21 220 16 151.80 1.33 579 21 222 18 151.62 1.34 580 21 21 3 796.87 0.49 581 21 21 12 152.09 1.42 582 21 218 13 151.94 1.35 583 21 219 12 152.01 1.37 584 21 222 15 151.80 1.33 585 21 21 8 152.38 1.32 586 21 218 10 152.08 1.38 587 21 219 11 152.07 1.38 588 21 21 3 1029.92 0.02 589 21 21 6 635.83 6.27 590 21 21 3 821.76 3.70 591 21 21 3 877.10 0.10 592 222 228 6 797.87 1.32 593 224 228 4 797.87 1.32 594 224 229 5 787.39 5.48 595 225 228 3 813.39 1.28 596 225 229 4 797.88 1.32 597 226 229 3 819.67 1.44 598 226 230 4 804.87 2.17 599 227 230 3 814.45 2.62 600 227 231 4 810.79 0.61 601 228 231 3 818.13 0.78 602 228 232 4 810.81 0.62 603 229 232 3 814.32 1.13 604 229 233 4 810.80 0.62 605 230 233 3 812.03 0.01 606 230 234 4 810.80 0.62 607 231 234 3 832.77 1.63 608 232 235 3 813.13 1.84 609 232 236 4 800.23 3.75 610 233 236 3 802.34 4.81