BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 244 241 32.45 0.20 2 3 245 242 32.11 0.06 3 3 247 244 30.20 0.35 4 3 248 245 21.32 0.18 5 3 250 247 16.94 0.12 6 4 248 244 25.12 0.20 7 5 116 111 44.41 0.64 8 5 240 235 44.44 0.63 9 5 244 239 38.36 0.47 10 5 247 242 32.15 0.07 11 6 117 111 44.44 0.65 12 6 118 112 39.80 1.77 13 6 247 241 33.47 0.09 14 8 12 4 851.12 1.75 15 8 13 5 848.75 2.93 16 8 117 109 192.22 73.34 17 8 118 110 44.36 0.64 18 9 12 3 863.65 1.43 19 9 13 4 856.13 2.33 20 10 13 3 869.66 3.58 21 10 14 4 854.22 3.29 22 11 14 3 872.38 2.94 23 11 15 4 863.65 1.43 24 11 90 79 593.80 0.66 25 12 15 3 870.40 1.95 26 12 16 4 865.46 1.43 27 12 90 78 591.98 1.56 28 12 91 79 591.95 1.56 29 12 92 80 591.98 1.56 30 12 95 83 590.68 2.19 31 12 117 105 518.39 2.08 32 13 16 3 865.47 1.43 33 13 17 4 865.46 1.44 34 13 117 104 518.99 1.77 35 14 17 3 865.46 1.44 36 14 18 4 861.19 3.56 37 15 18 3 875.64 3.66 38 15 19 4 852.32 0.90 39 15 88 73 605.66 0.74 40 15 90 75 606.24 0.59 41 15 91 76 605.63 0.75 42 16 19 3 868.65 4.07 43 16 20 4 852.60 0.81 44 16 91 75 606.24 0.59 45 16 99 83 603.27 0.48 46 16 115 99 537.57 0.07 47 16 117 101 523.40 0.24 48 17 20 3 880.47 1.24 49 17 21 4 861.51 0.50 50 18 21 3 875.24 1.38 51 18 22 4 860.71 0.90 52 18 88 70 610.16 0.66 53 19 22 3 866.68 5.06 54 19 23 4 857.35 3.40 55 19 88 69 610.09 0.66 56 19 91 72 610.05 0.67 57 19 96 77 607.06 0.24 58 19 99 80 607.04 0.24 59 20 23 3 880.46 8.15 60 20 24 4 857.71 3.22 61 20 99 79 607.06 0.24 62 20 102 82 607.04 0.24 63 20 103 83 606.97 0.27 64 20 106 86 604.15 0.30 65 21 24 3 886.96 4.90 66 21 25 4 858.13 3.43 67 22 25 3 872.35 0.12 68 22 26 4 854.20 5.39 69 22 84 62 612.26 0.36 70 22 88 66 612.34 0.41 71 23 26 3 874.88 1.15 72 23 27 4 840.34 0.22 73 23 81 58 612.44 0.40 74 23 84 61 612.35 0.37 75 23 96 73 610.68 0.78 76 23 99 76 610.38 0.65 77 23 100 77 610.24 0.58 78 23 103 80 610.16 0.69 79 24 27 3 887.13 4.59 80 24 28 4 852.43 5.00 81 24 103 79 610.17 0.70 82 24 106 82 607.18 1.04 83 24 107 83 600.13 0.76 84 25 28 3 881.76 0.73 85 25 29 4 851.47 4.51 86 26 29 3 871.44 0.55 87 26 30 4 851.47 4.51 88 26 32 6 826.59 1.12 89 26 80 54 612.82 0.43 90 26 81 55 612.67 0.49 91 26 84 58 612.51 0.42 92 27 30 3 859.41 2.79 93 27 31 4 849.25 3.41 94 27 32 5 822.79 3.00 95 27 33 6 799.02 7.02 96 27 36 9 741.79 13.94 97 27 100 73 611.89 0.18 98 27 103 76 610.69 0.73 99 27 104 77 610.57 0.72 100 27 107 80 603.26 0.14 101 28 31 3 864.67 2.83 102 28 107 79 605.66 0.46 103 30 80 50 613.38 0.37 104 31 184 153 310.75 0.43 105 32 35 3 881.72 3.92 106 32 36 4 850.26 6.33 107 32 80 48 616.46 0.60 108 33 36 3 866.38 3.75 109 33 37 4 858.25 2.33 110 33 104 71 611.50 0.74 111 33 107 74 610.24 0.67 112 33 108 75 606.15 0.17 113 33 165 132 312.33 0.78 114 34 37 3 873.90 5.49 115 34 38 4 860.88 1.01 116 34 165 131 312.34 0.78 117 34 166 132 312.31 0.78 118 34 183 149 310.85 0.42 119 34 184 150 310.78 0.42 120 35 38 3 864.18 2.10 121 35 39 4 856.78 1.59 122 35 73 38 645.33 5.52 123 35 76 41 645.25 5.49 124 35 77 42 645.21 5.47 125 35 80 45 645.20 5.50 126 35 172 137 312.72 0.57 127 36 39 3 881.13 1.08 128 36 40 4 868.34 5.31 129 36 77 41 645.27 5.50 130 36 80 44 645.20 5.51 131 36 81 45 645.19 5.51 132 36 100 64 613.64 0.35 133 36 101 65 613.62 0.35 134 36 104 68 611.76 0.80 135 37 40 3 875.35 1.80 136 37 41 4 856.36 1.39 137 37 101 64 613.63 0.35 138 37 104 67 611.81 0.78 139 37 105 68 611.79 0.79 140 37 162 125 312.62 0.69 141 37 165 128 312.35 0.79 142 37 186 149 310.61 0.41 143 37 235 198 165.55 16.07 144 37 239 202 153.00 20.39 145 38 41 3 881.12 1.09 146 38 42 4 869.05 4.95 147 38 70 32 645.90 5.38 148 38 72 34 645.85 5.38 149 38 74 36 645.38 5.50 150 39 42 3 863.21 0.12 151 39 43 4 852.78 3.21 152 39 60 21 645.62 5.46 153 39 74 35 645.38 5.50 154 39 77 38 645.36 5.50 155 39 78 39 645.36 5.50 156 39 81 42 645.28 5.53 157 39 97 58 616.31 0.86 158 40 43 3 862.69 0.14 159 40 44 4 850.69 4.25 160 40 97 57 616.37 0.89 161 40 98 58 616.29 0.88 162 40 101 61 613.64 0.35 163 41 44 3 881.63 0.62 164 41 45 4 854.98 2.10 165 41 132 91 454.15 2.40 166 41 145 104 333.20 0.40 167 41 149 108 333.19 0.40 168 41 242 201 138.56 20.16 169 42 45 3 875.12 2.64 170 42 46 4 855.00 2.11 171 42 60 18 645.77 5.42 172 42 70 28 646.00 5.40 173 42 74 32 645.68 5.44 174 42 131 89 461.74 1.39 175 43 55 12 646.76 5.25 176 43 56 13 646.43 5.40 177 43 60 17 645.75 5.42 178 43 97 54 616.47 0.91 179 43 98 55 616.43 0.91 180 44 47 3 912.36 1.34 181 44 56 12 646.40 5.31 182 44 98 54 616.45 0.91 183 44 242 198 138.85 20.30 184 44 243 199 138.33 20.06 185 45 131 86 461.79 1.36 186 45 149 104 333.19 0.41 187 46 54 8 647.37 5.25 188 46 55 9 647.32 5.24 189 46 65 19 646.16 5.34 190 46 126 80 538.63 0.48 191 46 130 84 493.05 3.26 192 46 131 85 469.77 2.42 193 47 54 7 647.38 5.24 194 47 55 8 647.35 5.25 195 47 56 9 647.36 5.25 196 47 130 83 535.42 1.45 197 47 148 101 333.60 0.45 198 47 149 102 333.37 0.49 199 47 245 198 138.20 20.08 200 47 250 203 85.31 0.06 201 48 51 3 891.68 0.51 202 48 52 4 649.67 4.94 203 48 130 82 535.48 1.43 204 48 148 100 333.60 0.45 205 49 146 97 333.69 0.43 206 49 147 98 333.69 0.43 207 49 148 99 333.68 0.43 208 49 150 101 333.58 0.45 209 51 130 79 539.83 0.57 210 52 250 198 85.78 0.29 211 53 126 73 546.35 3.13 212 53 130 77 539.15 0.25 213 54 126 72 539.13 0.27 214 55 59 4 798.90 12.05 215 55 126 71 539.11 0.27 216 56 94 38 616.98 0.89 217 56 245 189 133.34 22.04 218 56 246 190 83.18 1.09 219 57 94 37 616.99 0.88 220 57 120 63 547.32 3.29 221 57 121 64 547.13 3.25 222 57 122 65 547.07 3.28 223 57 246 189 83.18 1.09 224 58 61 3 904.83 2.14 225 58 93 35 616.99 0.86 226 58 94 36 616.98 0.86 227 58 121 63 547.06 3.28 228 59 62 3 886.24 1.99 229 59 65 6 839.21 4.44 230 59 122 63 547.08 3.27 231 59 124 65 544.41 2.86 232 59 125 66 538.53 0.57 233 59 126 67 538.62 0.53 234 60 65 5 848.70 0.29 235 60 66 6 831.90 8.70 236 60 71 11 648.78 4.83 237 60 74 14 646.10 5.39 238 60 78 18 646.03 5.38 239 60 97 37 616.84 0.81 240 61 66 5 866.52 1.41 241 61 78 17 646.04 5.38 242 61 82 21 646.02 5.38 243 61 93 32 617.05 0.89 244 61 97 36 616.93 0.85 245 62 65 3 883.51 3.64 246 62 66 4 872.81 1.72 247 63 66 3 873.02 1.76 248 63 67 4 872.81 1.72 249 64 67 3 897.70 1.65 250 64 68 4 888.23 1.58 251 65 68 3 897.53 1.56 252 65 71 6 755.31 7.84 253 65 125 60 541.71 1.85 254 65 126 61 538.86 0.43 255 66 71 5 724.26 1.77 256 66 74 8 647.77 5.23 257 66 75 9 646.10 5.42 258 66 78 12 647.12 4.91 259 68 71 3 870.38 1.48 260 68 72 4 864.83 1.30 261 68 128 60 535.82 1.28 262 69 72 3 883.72 0.26 263 71 74 3 854.55 3.85 264 72 172 100 312.60 0.51 265 73 76 3 862.45 3.17 266 73 77 4 866.00 4.92 267 73 172 99 312.64 0.53 268 74 77 3 877.62 3.34 269 74 78 4 877.51 3.39 270 75 78 3 877.52 3.39 271 75 79 4 877.51 3.40 272 76 79 3 877.53 3.39 273 76 80 4 875.98 2.62 274 76 172 96 312.66 0.54 275 77 80 3 885.68 0.70 276 77 81 4 879.96 0.63 277 78 81 3 881.50 1.41 278 78 82 4 879.95 0.63 279 78 97 19 616.76 0.79 280 79 82 3 894.20 4.01 281 79 83 4 898.95 1.64 282 80 83 3 949.86 2.12 283 81 84 3 894.43 2.88 284 81 85 4 866.22 2.31 285 81 96 15 616.77 0.80 286 81 97 16 616.82 0.82 287 81 100 19 615.97 1.15 288 82 85 3 877.10 1.99 289 82 86 4 866.24 2.31 290 83 86 3 884.31 1.48 291 83 87 4 869.16 0.85 292 84 87 3 885.39 2.02 293 84 88 4 871.14 1.84 294 84 96 12 650.88 4.62 295 85 88 3 880.21 4.62 296 85 89 4 871.13 1.84 297 85 91 6 837.86 4.25 298 85 92 7 813.02 16.66 299 85 93 8 680.43 15.84 300 85 96 11 629.69 5.43 301 86 89 3 871.14 1.84 302 88 91 3 891.06 1.87 303 88 96 8 734.48 8.31 304 91 96 5 849.18 0.07 305 92 95 3 867.90 5.76 306 92 96 4 861.46 6.04 307 92 119 27 547.29 3.29 308 92 121 29 547.03 3.29 309 93 96 3 882.85 3.41 310 93 97 4 864.28 5.71 311 93 121 28 547.05 3.29 312 94 97 3 882.84 3.41 313 94 98 4 866.46 4.63 314 94 119 25 547.32 3.29 315 94 121 27 547.04 3.29 316 95 98 3 871.44 2.14 317 95 99 4 866.46 4.63 318 95 115 20 547.74 3.19 319 95 117 22 547.38 3.29 320 95 118 23 547.35 3.27 321 95 119 24 547.29 3.29 322 96 99 3 876.48 4.67 323 96 100 4 866.46 4.63 324 97 100 3 871.43 2.14 325 97 101 4 865.46 4.00 326 98 101 3 874.64 0.60 327 98 102 4 869.18 2.12 328 98 115 17 547.75 3.19 329 98 119 21 547.36 3.26 330 98 243 145 133.34 22.03 331 99 102 3 879.72 3.16 332 99 103 4 869.15 2.12 333 99 115 16 547.66 3.16 334 100 103 3 875.04 5.50 335 100 104 4 857.39 1.19 336 101 104 3 871.86 3.46 337 101 105 4 861.89 1.06 338 101 239 138 152.94 20.43 339 102 105 3 879.74 3.04 340 102 106 4 866.60 6.09 341 102 113 11 547.77 3.17 342 102 115 13 547.69 3.17 343 102 239 137 153.13 20.53 344 103 106 3 862.78 1.05 345 103 107 4 855.63 2.77 346 104 107 3 889.65 0.53 347 104 108 4 857.53 1.57 348 105 108 3 884.88 2.92 349 105 109 4 852.25 1.07 350 105 110 5 735.72 8.84 351 105 111 6 735.72 8.84 352 105 113 8 548.01 3.21 353 105 186 81 310.36 0.39 354 105 235 130 163.99 16.86 355 105 239 134 153.22 20.48 356 106 109 3 879.46 8.97 357 106 112 6 547.82 3.12 358 108 165 57 312.30 0.77 359 108 184 76 310.73 0.41 360 108 185 77 310.50 0.43 361 108 186 78 310.38 0.39 362 109 186 77 310.39 0.38 363 109 232 123 171.10 16.73 364 110 186 76 310.36 0.39 365 110 232 122 171.03 16.70 366 111 186 75 310.38 0.38 367 111 232 121 170.94 16.71 368 111 235 124 167.34 15.20 369 111 236 125 159.53 18.73 370 113 235 122 164.19 16.96 371 113 236 123 159.16 18.61 372 113 239 126 153.24 20.50 373 113 240 127 152.94 20.43 374 114 240 126 153.08 20.54 375 115 119 4 1080.44 0.12 376 116 244 128 138.65 20.41 377 116 247 131 119.24 21.67 378 117 247 130 124.98 24.54 379 118 247 129 121.23 22.67 380 119 243 124 144.93 23.43 381 119 244 125 134.40 22.51 382 119 246 127 76.61 0.35 383 119 247 128 76.19 0.18 384 126 130 4 892.05 0.21 385 127 130 3 927.02 0.83 386 130 144 14 333.69 0.43 387 131 144 13 333.69 0.43 388 131 149 18 333.70 0.43 389 132 140 8 334.56 0.74 390 132 144 12 333.71 0.42 391 133 136 3 869.70 4.77 392 133 140 7 535.61 81.53 393 133 207 74 257.62 5.75 394 134 175 41 312.89 0.56 395 134 205 71 258.38 6.03 396 134 206 72 258.36 6.03 397 134 207 73 257.06 5.46 398 135 175 40 313.14 0.67 399 135 206 71 258.38 6.03 400 136 140 4 835.20 9.96 401 136 206 70 258.44 6.08 402 136 207 71 258.31 6.05 403 137 140 3 879.56 3.89 404 137 141 4 844.59 9.79 405 137 210 73 253.88 6.29 406 138 141 3 864.96 2.19 407 138 142 4 866.98 0.86 408 138 210 72 253.89 6.29 409 138 214 76 245.27 6.17 410 138 217 79 213.59 6.12 411 139 142 3 866.98 0.86 412 139 143 4 866.99 0.86 413 140 143 3 866.99 0.86 414 140 144 4 866.99 0.86 415 141 144 3 874.24 2.18 416 141 145 4 848.26 8.50 417 141 156 15 334.00 0.70 418 141 207 66 257.67 5.77 419 141 210 69 253.80 6.24 420 141 211 70 253.78 6.24 421 141 217 76 213.56 6.12 422 142 146 4 780.08 8.13 423 142 147 5 766.35 1.27 424 142 153 11 335.61 0.07 425 142 156 14 334.38 0.52 426 142 214 72 245.18 6.03 427 142 216 74 213.65 6.09 428 142 217 75 213.57 6.12 429 143 147 4 705.56 22.53 430 143 148 5 704.72 22.95 431 144 148 4 708.30 21.16 432 144 149 5 708.29 21.16 433 145 149 4 701.91 24.36 434 145 150 5 701.86 24.38 435 145 151 6 382.00 5.92 436 145 156 11 335.32 0.09 437 145 159 14 335.32 0.09 438 146 150 4 583.56 83.53 439 146 151 5 382.50 5.71 440 149 242 93 152.66 20.30 441 149 245 96 141.62 18.42 442 151 155 4 841.46 9.58 443 151 159 8 800.59 1.21 444 151 241 90 157.41 18.48 445 151 242 91 154.69 19.77 446 152 155 3 874.66 0.85 447 152 156 4 868.80 3.79 448 152 241 89 159.83 17.91 449 153 156 3 868.81 3.79 450 153 157 4 868.80 3.79 451 153 216 63 213.42 6.11 452 153 217 64 213.41 6.10 453 153 220 67 211.89 5.74 454 154 157 3 881.07 0.82 455 154 158 4 860.91 0.14 456 154 220 66 210.56 5.07 457 154 224 70 203.93 6.90 458 154 234 80 170.87 16.68 459 154 237 83 168.42 15.54 460 155 158 3 877.57 1.96 461 155 159 4 862.25 3.27 462 155 238 83 159.80 17.91 463 155 241 86 159.15 17.58 464 156 159 3 875.66 1.01 465 156 160 4 864.21 2.32 466 156 211 55 246.41 6.26 467 156 217 61 213.41 6.10 468 157 160 3 877.58 1.97 469 157 161 4 866.61 3.52 470 157 191 34 306.99 1.29 471 157 211 54 250.15 4.49 472 157 217 60 213.40 6.09 473 157 220 63 210.42 5.14 474 157 221 64 210.11 5.05 475 157 224 67 199.95 8.86 476 158 161 3 880.00 3.00 477 158 224 66 197.97 8.55 478 158 231 73 171.15 16.80 479 158 234 76 169.08 15.83 480 158 235 77 167.93 15.29 481 158 238 80 163.06 16.47 482 159 162 3 892.34 3.17 483 159 235 76 167.41 15.14 484 159 238 79 158.31 18.08 485 159 239 80 158.16 18.03 486 159 242 83 151.84 19.79 487 160 163 3 898.47 0.10 488 160 190 30 307.01 1.28 489 160 211 51 252.42 5.54 490 161 164 3 881.96 0.26 491 161 190 29 307.00 1.29 492 161 191 30 307.00 1.29 493 161 231 70 171.38 16.68 494 162 165 3 902.96 1.78 495 162 186 24 310.75 0.42 496 162 235 73 167.32 15.19 497 163 179 16 311.86 0.17 498 163 190 27 307.00 1.29 499 163 205 42 267.30 6.08 500 163 208 45 256.54 5.24 501 164 180 16 311.84 0.15 502 164 183 19 310.87 0.42 503 164 186 22 310.76 0.42 504 164 187 23 310.75 0.42 505 164 189 25 310.17 0.43 506 164 190 26 305.98 1.80 507 165 183 18 310.85 0.42 508 165 184 19 310.83 0.42 509 165 185 20 310.80 0.43 510 165 186 21 310.76 0.42 511 166 178 12 314.15 0.48 512 166 179 13 314.14 0.48 513 166 183 17 310.86 0.42 514 167 177 10 314.69 0.70 515 167 178 11 314.15 0.48 516 167 179 12 314.15 0.48 517 168 173 5 559.37 69.73 518 168 175 7 333.98 10.12 519 168 176 8 314.87 0.68 520 168 177 9 314.83 0.67 521 168 179 11 314.73 0.70 522 168 203 35 269.17 6.66 523 169 172 3 856.30 3.20 524 169 173 4 818.25 15.71 525 169 174 5 367.07 6.33 526 169 175 6 347.64 3.38 527 169 176 7 333.73 10.10 528 170 174 4 347.67 3.36 529 170 175 5 347.67 3.37 530 170 176 6 347.64 3.37 531 175 203 28 269.18 6.66 532 175 205 30 268.84 6.70 533 175 206 31 268.84 6.70 534 176 203 27 269.19 6.66 535 177 203 26 269.18 6.66 536 178 183 5 577.31 109.55 537 178 201 23 269.20 6.65 538 178 202 24 269.19 6.65 539 178 203 25 269.13 6.67 540 179 190 11 310.20 0.30 541 179 193 14 296.10 0.82 542 179 202 23 269.19 6.66 543 179 203 24 269.13 6.68 544 179 204 25 269.00 6.62 545 179 205 26 269.12 6.69 546 179 208 29 256.64 5.23 547 180 183 3 875.52 4.99 548 180 189 9 310.62 0.41 549 180 190 10 310.44 0.38 550 180 193 13 299.50 0.83 551 180 202 22 269.19 6.66 552 181 202 21 269.19 6.66 553 182 185 3 868.97 0.10 554 182 187 5 784.10 6.83 555 182 189 7 310.56 0.41 556 186 231 45 171.44 16.66 557 186 232 46 171.09 16.72 558 186 235 49 167.45 15.12 559 187 228 41 171.44 16.66 560 187 231 44 171.44 16.66 561 187 232 45 171.37 16.66 562 188 191 3 877.76 0.96 563 188 192 4 876.46 0.68 564 188 224 36 197.50 8.56 565 188 225 37 197.25 8.54 566 188 228 40 171.44 16.66 567 188 231 43 171.44 16.66 568 189 192 3 876.46 0.68 569 189 193 4 870.87 3.46 570 190 193 3 876.48 0.69 571 190 194 4 872.62 2.60 572 190 208 18 256.62 5.25 573 190 211 21 255.94 5.17 574 191 194 3 882.30 2.23 575 191 195 4 872.62 2.60 576 191 221 30 208.28 5.91 577 191 224 33 198.47 8.12 578 191 225 34 197.47 8.58 579 191 231 40 175.11 14.83 580 192 195 3 877.46 0.18 581 192 196 4 866.06 1.09 582 192 225 33 197.64 8.49 583 193 196 3 875.95 0.94 584 193 197 4 866.06 1.09 585 193 202 9 269.28 6.65 586 193 204 11 269.14 6.70 587 194 197 3 888.78 0.93 588 194 204 10 269.00 6.62 589 194 208 14 256.67 5.25 590 194 209 15 256.61 5.24 591 194 211 17 256.54 5.22 592 194 212 18 250.96 3.74 593 194 221 27 210.66 5.25 594 195 212 17 252.37 3.38 595 195 218 23 213.47 6.08 596 195 221 26 210.84 5.18 597 195 222 27 210.63 5.25 598 195 225 30 202.28 7.71 599 197 204 7 269.30 6.64 600 198 202 4 849.82 7.12 601 198 204 6 332.53 24.98 602 198 209 11 267.27 6.16 603 199 202 3 876.27 1.10 604 204 208 4 843.00 0.54 605 204 209 5 843.00 0.53 606 205 208 3 852.46 0.04 607 205 209 4 843.15 0.48 608 206 209 3 852.27 4.07 609 206 210 4 852.21 4.04 610 207 210 3 865.19 0.46 611 208 211 3 879.72 2.25 612 209 212 3 879.77 8.15 613 209 213 4 870.51 1.07 614 210 213 3 891.96 2.06 615 210 214 4 865.90 1.24 616 211 217 6 274.59 24.48 617 211 218 7 366.73 21.59 618 211 221 10 272.31 25.61 619 212 218 6 366.74 21.59 620 212 221 9 273.11 25.23 621 214 217 3 875.70 4.88 622 214 218 4 823.33 9.21 623 215 219 4 876.55 4.46 624 216 219 3 908.16 1.79 625 216 220 4 868.44 0.41 626 217 220 3 880.72 2.33 627 217 221 4 860.90 3.34 628 218 221 3 863.62 1.99 629 218 222 4 860.89 3.34 630 219 222 3 873.17 8.81 631 219 223 4 856.39 4.88 632 220 223 3 877.51 6.65 633 220 224 4 856.15 4.76 634 221 224 3 872.10 2.77 635 221 225 4 855.55 5.07 636 222 225 3 879.08 0.72 637 222 226 4 855.55 5.07 638 223 226 3 860.86 7.73 639 223 227 4 854.17 5.75 640 223 230 7 708.28 2.75 641 223 234 11 688.43 6.15 642 224 227 3 855.28 6.28 643 224 230 6 708.31 2.75 644 224 231 7 688.36 6.17 645 224 234 10 688.38 6.17 646 227 230 3 868.07 1.20 647 227 231 4 852.52 7.27 648 228 231 3 861.39 2.84 649 228 232 4 832.66 2.66 650 229 232 3 859.10 1.20 651 229 233 4 832.66 2.66 652 230 233 3 872.88 3.54 653 230 234 4 839.03 0.52 654 231 234 3 876.78 5.50 655 231 235 4 839.01 0.52 656 232 235 3 866.97 9.78 657 232 236 4 841.70 0.81 658 233 236 3 876.30 5.11 659 233 237 4 842.77 0.30 660 234 237 3 866.98 9.77 661 234 238 4 846.94 2.38 662 235 238 3 873.89 3.40 663 235 239 4 842.76 0.30 664 236 239 3 866.19 7.25 665 236 240 4 841.80 0.78 666 237 240 3 872.31 4.18 667 237 241 4 840.12 0.06 668 238 241 3 867.62 6.52 669 238 242 4 839.22 0.50 670 239 242 3 867.64 6.50 671 239 243 4 833.24 3.69 672 240 243 3 875.73 1.92 673 240 244 4 861.01 5.30 674 241 244 3 875.95 2.02 675 241 245 4 861.05 5.32 676 242 245 3 875.62 1.86 677 244 247 3 848.68 1.53 678 247 250 3 864.62 1.46