BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 8 127 120 46.90 0.31 2 8 128 121 31.25 1.28 3 9 127 119 46.90 0.31 4 10 127 118 48.16 0.43 5 10 128 119 48.16 0.43 6 11 14 3 864.96 3.77 7 11 125 115 48.43 0.32 8 11 126 116 48.43 0.31 9 11 127 117 48.41 0.32 10 12 23 11 48.45 0.30 11 12 128 117 48.44 0.30 12 12 129 118 48.44 0.30 13 13 21 8 48.51 0.29 14 13 23 10 48.45 0.30 15 13 24 11 48.47 0.29 16 13 124 112 48.47 0.30 17 13 131 119 48.45 0.30 18 14 21 7 48.62 0.33 19 15 20 5 417.84 4.05 20 15 21 6 49.40 0.06 21 16 19 3 790.45 6.05 22 16 20 4 655.70 14.78 23 20 25 5 793.18 10.03 24 21 24 3 834.72 0.14 25 21 25 4 823.85 5.29 26 22 25 3 823.85 5.29 27 22 26 4 823.85 5.29 28 23 26 3 823.85 5.29 29 23 27 4 798.21 7.52 30 23 131 109 372.88 0.60 31 24 27 3 821.21 0.49 32 24 28 4 807.96 2.64 33 24 124 101 372.92 0.60 34 24 131 108 375.27 0.39 35 24 154 132 372.25 0.82 36 25 28 3 827.94 2.92 37 25 29 4 807.98 2.62 38 26 29 3 823.95 6.24 39 26 30 4 814.02 1.28 40 27 30 3 823.95 6.24 41 27 31 4 807.25 2.10 42 27 131 105 381.98 0.28 43 27 132 106 382.16 0.18 44 27 133 107 381.96 0.28 45 27 153 128 381.90 0.28 46 28 31 3 820.07 4.31 47 28 32 4 811.84 0.20 48 28 152 126 381.91 0.29 49 28 153 127 381.95 0.28 50 29 32 3 818.94 1.17 51 29 33 4 808.73 1.75 52 30 33 3 829.72 1.07 53 30 34 4 808.82 1.72 54 31 34 3 820.22 1.82 55 31 79 48 382.60 0.03 56 31 80 49 382.60 0.03 57 31 133 103 382.59 0.03 58 31 134 104 382.56 0.03 59 31 135 105 382.54 0.02 60 31 153 124 382.54 0.02 61 31 166 137 338.81 0.85 62 32 166 136 338.66 0.95 63 32 167 137 338.47 1.02 64 32 169 139 337.81 1.31 65 33 167 136 338.63 0.94 66 34 79 45 382.88 0.03 67 34 80 46 383.02 0.02 68 34 166 134 339.37 1.11 69 34 167 135 339.13 1.01 70 35 51 16 406.06 6.82 71 35 53 18 406.02 6.80 72 35 66 31 405.65 6.67 73 35 79 44 386.47 0.96 74 36 50 14 406.11 6.83 75 36 164 130 340.14 1.08 76 36 165 131 340.10 1.08 77 36 166 132 339.72 0.99 78 36 167 133 339.47 1.11 79 36 168 134 339.42 1.09 80 36 200 166 257.25 1.41 81 37 49 12 406.39 6.90 82 37 50 13 406.12 6.83 83 37 77 40 404.89 6.43 84 37 78 41 401.88 7.11 85 37 79 42 401.44 6.90 86 37 137 101 404.75 6.47 87 37 164 129 340.16 1.09 88 37 165 130 340.12 1.09 89 38 48 10 406.42 6.91 90 38 49 11 406.41 6.90 91 38 137 100 405.45 6.59 92 38 163 127 340.47 1.21 93 38 164 128 340.16 1.09 94 38 193 157 258.67 1.39 95 38 196 160 258.54 1.41 96 38 197 161 258.35 1.33 97 39 47 8 411.72 4.36 98 39 48 9 406.42 6.91 99 39 137 99 405.60 6.58 100 39 148 111 406.38 6.92 101 39 162 125 368.55 0.59 102 39 163 126 351.10 0.27 103 40 46 6 412.62 3.97 104 40 47 7 412.52 3.99 105 40 148 110 406.62 6.91 106 40 161 123 381.19 0.07 107 40 162 124 368.62 0.62 108 40 176 138 329.79 2.00 109 40 180 142 305.03 0.58 110 40 189 151 259.31 1.26 111 40 192 154 259.18 1.19 112 40 193 155 258.70 1.38 113 41 45 4 742.34 2.56 114 41 46 5 415.15 5.24 115 41 147 107 407.02 6.79 116 41 14 108 406.99 6.79 117 41 148 109 406.86 6.80 118 41 160 121 383.05 0.04 119 41 161 122 381.61 0.28 120 41 180 141 314.56 4.17 121 42 45 3 777.87 1.94 122 42 184 144 271.05 1.79 123 42 185 145 271.02 1.78 124 42 186 146 264.00 1.70 125 42 189 149 259.43 1.23 126 43 182 141 304.74 0.47 127 43 183 142 297.57 3.06 128 43 184 143 292.42 5.58 129 44 147 104 412.08 5.86 130 44 185 143 273.58 0.55 131 44 186 144 271.04 1.78 132 44 216 174 256.72 1.55 133 45 147 103 412.11 5.87 134 45 186 143 263.73 1.56 135 45 215 172 256.72 1.55 136 45 216 173 256.65 1.54 137 46 139 94 412.10 5.87 138 46 147 102 412.16 5.86 139 46 14 103 412.13 5.87 140 46 148 104 412.09 5.87 141 46 186 142 263.17 1.28 142 46 214 170 256.74 1.55 143 46 215 171 256.67 1.54 144 47 189 144 259.43 1.23 145 47 190 145 259.39 1.21 146 47 193 148 259.13 1.17 147 47 212 167 257.13 1.44 148 47 213 168 256.91 1.53 149 47 214 169 256.67 1.54 150 48 67 19 412.25 5.94 151 48 75 27 412.27 5.88 152 48 76 28 412.24 5.90 153 48 77 29 412.19 5.91 154 48 139 92 412.24 5.88 155 48 148 102 412.24 5.88 156 48 212 166 257.06 1.45 157 48 213 167 256.75 1.53 158 48 215 169 256.68 1.53 159 49 197 150 258.54 1.41 160 49 211 164 257.10 1.44 161 49 212 165 256.36 1.34 162 50 66 16 412.55 5.95 163 50 67 17 412.57 5.96 164 50 77 27 412.28 5.88 165 50 197 149 258.42 1.35 166 50 210 162 257.19 1.40 167 50 211 163 256.94 1.44 168 51 66 15 412.67 5.96 169 51 202 152 257.69 1.39 170 51 20 160 257.27 1.40 171 51 210 161 257.18 1.39 172 52 61 9 413.19 5.86 173 52 62 10 413.20 5.86 174 52 64 12 413.05 5.92 175 52 66 14 412.71 5.98 176 52 209 158 257.31 1.40 177 52 20 159 257.24 1.39 178 52 210 160 257.20 1.39 179 52 211 161 257.17 1.39 180 56 59 3 797.16 2.44 181 60 63 3 826.33 4.90 182 61 64 3 821.66 0.18 183 65 211 148 256.85 1.49 184 65 223 175 120.89 0.40 185 66 77 11 508.43 1.04 186 66 78 12 508.09 1.22 187 66 85 19 507.88 1.31 188 66 211 147 256.85 1.49 189 67 76 9 526.50 1.51 190 67 77 10 514.78 4.21 191 67 85 18 528.11 2.30 192 67 211 146 256.92 1.52 193 67 213 148 256.73 1.54 194 67 221 171 129.56 0.10 195 67 223 173 120.88 0.39 196 68 75 7 578.17 16.41 197 68 76 8 538.55 0.40 198 68 85 17 538.54 0.40 199 68 89 21 538.72 0.23 200 68 93 25 543.23 1.40 201 68 221 170 129.55 0.10 202 69 72 3 867.26 1.82 203 69 74 5 662.35 12.58 204 69 75 6 578.18 16.41 205 69 93 24 543.25 1.41 206 69 221 169 129.54 0.10 207 70 74 4 732.94 13.22 208 70 75 5 616.75 1.47 209 70 76 6 579.89 16.96 210 70 89 19 543.21 1.39 211 70 92 22 543.23 1.40 212 70 93 23 543.23 1.41 213 70 112 43 548.78 1.33 214 71 74 3 792.86 5.21 215 71 96 25 572.85 2.41 216 71 106 36 607.28 0.38 217 71 107 37 606.87 0.21 218 71 112 42 578.62 0.48 219 72 106 35 608.29 0.30 220 72 219 164 129.88 0.21 221 72 220 165 129.95 0.17 222 72 221 166 129.75 0.14 223 73 140 68 608.23 0.35 224 73 141 69 608.20 0.35 225 73 215 144 256.72 1.56 226 73 219 163 129.96 0.18 227 73 220 164 129.88 0.20 228 73 221 165 129.57 0.10 229 74 107 34 608.44 0.24 230 74 109 36 608.22 0.35 231 74 112 39 608.29 0.30 232 74 138 65 608.57 0.31 233 74 139 66 608.52 0.34 234 74 140 67 608.29 0.39 235 75 138 64 608.58 0.32 236 75 139 65 608.58 0.34 237 75 221 163 129.32 0.01 238 76 85 9 610.19 0.17 239 76 137 62 608.61 0.32 240 76 138 63 608.59 0.32 241 77 85 8 610.23 0.15 242 77 136 60 608.91 0.32 243 77 137 61 608.65 0.31 244 78 82 4 851.95 0.88 245 78 85 7 610.32 0.17 246 78 134 57 609.23 0.30 247 78 135 58 609.11 0.29 248 78 136 59 608.92 0.32 249 78 165 89 339.97 1.11 250 79 134 56 609.27 0.29 251 79 135 57 609.19 0.28 252 79 165 88 339.48 1.10 253 79 166 89 339.16 1.01 254 80 133 54 610.00 0.62 255 80 134 55 609.27 0.29 256 81 84 3 836.52 3.98 257 81 85 4 813.58 3.00 258 81 130 50 613.23 2.19 259 81 133 53 611.68 1.46 260 81 134 54 610.00 0.62 261 82 85 3 813.59 3.00 262 82 86 4 813.62 2.98 263 83 86 3 818.13 0.73 264 83 87 4 818.14 0.73 265 84 87 3 844.25 1.47 266 84 88 4 818.14 0.72 267 84 130 47 613.47 2.15 268 84 132 49 612.85 2.02 269 84 133 50 612.66 1.93 270 84 134 51 612.62 1.92 271 85 88 3 818.22 2.56 272 85 89 4 814.89 0.90 273 85 134 50 612.65 1.94 274 85 136 52 612.59 1.97 275 86 89 3 816.34 1.62 276 86 90 4 814.88 0.91 277 87 90 3 817.61 2.25 278 87 91 4 808.05 1.15 279 87 132 46 613.49 2.08 280 88 91 3 822.34 0.11 281 88 92 4 805.72 1.02 282 88 119 32 613.82 2.08 283 88 120 33 613.65 2.16 284 88 132 45 613.51 2.09 285 88 134 47 613.37 2.04 286 88 136 49 613.10 2.04 287 89 92 3 814.88 0.26 288 89 93 4 809.03 2.67 289 90 93 3 815.74 0.69 290 90 94 4 813.73 0.36 291 91 94 3 828.44 3.00 292 91 95 4 813.72 0.36 293 91 119 29 613.87 2.10 294 92 95 3 819.89 2.72 295 92 96 4 813.72 0.36 296 92 112 21 614.06 2.00 297 92 116 25 613.86 2.10 298 93 96 3 829.31 4.91 299 93 97 4 813.72 0.36 300 94 97 3 827.79 3.72 301 94 98 4 813.16 0.62 302 95 98 3 829.54 4.60 303 95 99 4 798.19 6.86 304 95 112 18 614.50 2.19 305 95 115 21 614.07 2.14 306 96 100 4 791.71 14.31 307 96 101 5 746.97 5.59 308 96 107 12 616.45 2.13 309 96 112 17 614.51 2.19 310 97 100 3 829.54 4.61 311 97 101 4 746.84 5.65 312 97 102 5 746.02 5.68 313 97 106 10 619.44 2.45 314 98 102 4 779.10 1.51 315 98 10 5 769.79 6.17 316 99 10 4 812.92 2.54 317 100 10 3 822.60 6.01 318 100 103 4 802.68 7.67 319 100 105 6 705.49 2.21 320 100 106 7 628.19 3.19 321 100 107 8 619.48 2.47 322 100 108 9 619.50 2.48 323 100 111 12 617.61 1.70 324 101 103 3 829.32 2.65 325 101 104 4 721.03 7.88 326 101 105 5 630.31 2.93 327 101 106 6 619.50 2.48 328 107 111 4 811.38 4.59 329 107 112 5 765.22 10.22 330 108 111 3 836.46 0.46 331 108 112 4 814.60 1.76 332 108 142 34 611.94 2.04 333 108 145 37 609.28 0.76 334 109 112 3 826.76 3.72 335 109 113 4 815.41 1.37 336 109 138 29 613.17 2.21 337 109 140 31 612.64 2.33 338 109 141 32 612.57 2.29 339 109 142 33 612.54 2.30 340 109 14 39 609.03 0.70 341 109 149 41 608.99 0.71 342 110 113 3 819.94 0.31 343 110 114 4 814.62 1.78 344 110 145 35 609.38 0.72 345 110 149 40 608.97 0.70 346 111 114 3 814.60 1.77 347 111 115 4 814.66 1.80 348 112 115 3 814.72 1.78 349 112 116 4 815.76 1.66 350 113 116 3 816.52 1.28 351 113 117 4 815.82 1.62 352 113 138 25 613.10 2.18 353 113 149 37 608.88 0.65 354 113 151 39 608.46 0.63 355 113 157 45 494.44 1.15 356 114 117 3 815.90 1.58 357 114 118 4 815.83 1.62 358 114 157 44 494.51 1.18 359 115 118 3 819.50 0.21 360 115 119 4 819.50 0.21 361 116 119 3 826.39 0.03 362 116 120 4 819.51 0.21 363 116 136 20 613.29 2.15 364 116 151 36 608.62 0.57 365 117 120 3 824.44 2.25 366 117 121 4 819.50 0.21 367 117 151 35 608.63 0.56 368 117 155 39 525.38 1.74 369 117 157 41 494.70 1.17 370 118 121 3 820.75 0.40 371 118 122 4 819.27 0.32 372 119 122 3 830.19 2.04 373 119 123 4 819.31 0.33 374 119 132 13 614.25 1.87 375 120 123 3 827.48 3.39 376 120 124 4 819.29 0.33 377 120 132 12 614.08 1.96 378 120 134 14 613.94 2.01 379 120 136 16 613.66 2.06 380 120 151 32 608.68 0.54 381 120 152 33 608.61 0.53 382 120 153 34 608.24 0.56 383 120 155 36 557.69 3.52 384 121 124 3 824.55 1.95 385 121 125 4 819.64 0.51 386 121 154 34 608.34 0.57 387 121 155 35 595.64 0.14 388 122 125 3 833.11 7.30 389 122 126 4 800.47 3.10 390 123 129 6 620.80 1.05 391 123 130 7 614.43 1.91 392 123 131 8 614.28 1.88 393 123 132 9 614.28 1.88 394 124 131 7 614.29 1.88 395 124 153 30 608.61 0.49 396 124 154 31 608.52 0.49 397 126 129 3 861.76 3.25 398 130 133 3 1030.00 0.00 399 132 153 22 608.22 0.49 400 134 153 20 608.19 0.48 401 135 151 17 608.29 0.49 402 135 152 18 608.19 0.47 403 135 153 19 608.18 0.47 404 135 165 31 339.48 1.10 405 136 150 15 608.34 0.51 406 136 151 16 608.25 0.51 407 137 148 12 608.83 0.64 408 137 149 13 608.63 0.66 409 137 150 14 608.47 0.61 410 137 163 27 340.36 1.18 411 137 165 29 340.05 1.09 412 138 148 11 608.83 0.66 413 138 149 12 608.68 0.68 414 138 151 14 608.59 0.67 415 139 14 9 608.98 0.71 416 139 148 10 608.69 0.71 417 139 215 77 256.21 1.31 418 140 147 7 609.07 0.71 419 140 14 8 609.06 0.71 420 140 215 76 256.67 1.54 421 141 146 5 609.09 0.71 422 141 147 6 609.07 0.71 423 141 215 75 256.77 1.59 424 141 220 95 129.99 0.15 425 142 145 3 816.15 0.44 426 142 146 4 625.32 1.90 427 147 215 69 256.71 1.55 428 147 216 70 256.69 1.54 429 147 220 89 130.00 0.15 430 148 159 11 477.26 1.96 431 148 160 12 463.54 0.62 432 148 162 14 450.56 2.81 433 149 157 8 494.42 1.15 434 149 158 9 485.69 5.08 435 149 159 10 480.61 3.64 436 150 157 7 494.15 1.01 437 150 158 8 486.13 5.00 438 150 160 10 483.56 5.11 439 150 163 13 376.74 17.09 440 150 165 15 339.98 1.11 441 151 156 5 561.25 34.29 442 151 157 6 494.42 1.15 443 152 155 3 823.62 1.61 444 152 156 4 641.36 10.21 445 152 158 6 628.83 8.28 446 160 163 3 861.75 0.83 447 161 180 19 304.80 0.49 448 162 176 14 329.16 1.73 449 162 180 18 304.81 0.49 450 163 173 10 329.88 1.98 451 163 176 13 328.76 1.93 452 164 169 5 338.44 1.01 453 164 172 8 331.49 1.45 454 164 173 9 329.88 1.98 455 165 169 4 439.51 12.54 456 166 169 3 805.74 2.25 457 168 171 3 825.85 2.00 458 168 172 4 807.89 1.10 459 169 172 3 808.93 1.60 460 169 173 4 807.89 1.09 461 170 173 3 816.11 1.36 462 170 174 4 814.50 2.21 463 171 174 3 822.35 1.05 464 171 175 4 815.14 2.54 465 172 175 3 822.46 1.00 466 172 176 4 822.35 1.06 467 172 196 24 258.12 1.30 468 173 176 3 824.96 0.19 469 173 177 4 822.36 1.06 470 174 177 3 824.91 0.21 471 174 178 4 822.36 1.07 472 175 178 3 824.56 0.23 473 175 179 4 822.34 1.07 474 175 195 20 258.56 1.44 475 175 196 21 258.48 1.44 476 176 179 3 821.99 1.51 477 176 180 4 810.38 3.43 478 176 192 16 258.74 1.41 479 176 196 20 258.11 1.26 480 179 192 13 258.88 1.46 481 179 195 16 258.84 1.48 482 180 184 4 815.08 2.65 483 181 184 3 846.32 3.14 484 184 188 4 787.76 3.62 485 185 188 3 817.81 1.24 486 185 189 4 805.28 1.89 487 186 189 3 814.34 2.65 488 186 190 4 816.92 3.93 489 186 214 28 257.14 1.43 490 186 216 30 256.78 1.52 491 187 190 3 816.93 3.93 492 187 191 4 816.91 3.93 493 187 232 60 90.38 0.40 494 188 191 3 816.94 3.93 495 188 192 4 813.56 5.61 496 189 192 3 820.11 2.33 497 189 193 4 808.81 3.25 498 190 193 3 815.37 0.03 499 190 194 4 808.80 3.25 500 190 212 22 257.24 1.42 501 190 214 24 257.04 1.46 502 190 224 49 128.16 0.32 503 190 232 57 88.95 0.28 504 191 194 3 812.67 1.48 505 191 195 4 811.28 2.17 506 191 235 59 81.02 0.47 507 191 236 60 58.76 3.08 508 192 195 3 816.96 0.85 509 192 196 4 811.32 2.15 510 193 196 3 817.28 0.69 511 193 197 4 820.98 2.54 512 193 212 19 257.12 1.44 513 194 197 3 821.00 2.54 514 194 198 4 820.98 2.55 515 194 206 11 257.49 1.40 516 194 210 16 257.25 1.41 517 194 212 18 257.09 1.44 518 194 229 50 93.06 1.54 519 194 232 53 88.12 0.13 520 194 233 54 87.42 0.20 521 194 236 57 55.27 1.33 522 195 198 3 826.62 0.27 523 195 199 4 826.58 0.24 524 195 236 56 54.57 1.68 525 196 199 3 834.63 1.03 526 197 200 3 826.56 0.26 527 197 202 4 820.96 2.54 528 197 206 8 332.61 36.23 529 197 210 13 257.17 1.43 530 198 202 3 821.48 2.81 531 198 206 7 257.31 1.42 532 198 236 53 54.95 1.17 533 202 205 3 913.49 2.23 534 202 206 4 798.50 8.80 535 202 210 9 257.19 1.42 536 205 20 5 337.42 37.41 537 206 20 4 751.39 0.78 538 206 210 5 448.06 92.72 539 206 233 43 88.25 0.20 540 207 20 3 830.62 3.06 541 207 210 4 736.73 8.11 542 207 230 39 93.04 1.55 543 207 233 42 88.26 0.20 544 208 226 34 93.09 1.55 545 208 229 37 93.05 1.55 546 208 230 38 93.05 1.55 547 208 233 41 88.39 0.27 548 209 226 33 96.12 1.02 549 210 229 34 93.06 1.54 550 211 223 27 120.96 0.42 551 212 223 26 121.92 0.22 552 212 224 27 120.78 0.36 553 212 229 32 93.08 1.53 554 213 221 23 129.54 0.12 555 213 222 24 129.15 0.01 556 213 223 25 122.17 0.35 557 213 224 26 120.92 0.40 558 214 220 21 129.90 0.21 559 214 221 22 129.55 0.12 560 214 222 23 129.28 0.04 561 214 224 25 128.19 0.30 562 215 220 20 129.88 0.21 563 215 221 21 129.71 0.21 564 216 220 19 130.03 0.18 565 217 220 18 130.09 0.18 566 21 21 14 131.56 0.33 567 21 218 15 130.97 0.05 568 21 219 16 130.82 0.04 569 21 220 17 130.13 0.21 570 21 21 3 812.98 5.61 571 21 21 4 797.74 0.94 572 21 21 13 131.75 0.32 573 21 218 14 131.55 0.33 574 21 219 15 130.97 0.04 575 21 220 16 130.88 0.04 576 21 222 18 130.52 0.03 577 21 21 3 801.79 2.46 578 21 21 12 131.74 0.31 579 21 218 13 131.60 0.36 580 21 219 12 131.79 0.30 581 21 222 15 131.01 0.07 582 21 21 8 132.01 0.30 583 21 218 10 131.80 0.30 584 21 219 11 131.80 0.30 585 21 21 3 1029.99 0.00 586 21 21 6 635.83 6.27 587 21 21 3 821.76 3.70 588 21 21 3 877.10 0.10 589 222 228 6 797.97 1.37 590 224 228 4 796.28 2.12 591 224 229 5 795.39 1.69 592 225 228 3 813.39 1.28 593 225 229 4 801.31 3.04 594 226 229 3 819.67 1.44 595 226 230 4 808.30 0.46 596 227 230 3 814.62 2.53 597 227 231 4 808.67 0.45 598 228 231 3 818.13 0.78 599 228 232 4 808.68 0.44 600 229 232 3 813.65 1.47 601 229 233 4 808.68 0.44 602 230 233 3 811.38 0.33 603 230 234 4 808.68 0.44 604 231 234 3 831.69 2.17 605 232 235 3 828.73 3.65 606 232 236 4 810.55 0.70 607 233 236 3 806.08 2.94