BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1568 1597 29 438.80 86.78 2 1568 1605 37 442.66 88.53 3 1569 1573 4 806.82 4.22 4 1569 1605 36 443.52 88.91 5 1570 1573 3 862.14 2.98 6 1570 1588 18 443.85 89.17 7 1570 1591 21 442.47 88.49 8 1571 1586 15 445.11 89.45 9 1571 1587 16 444.05 89.27 10 1571 1588 17 443.85 89.17 11 1571 1591 20 442.48 88.49 12 1571 1592 21 442.48 88.48 13 1571 1597 26 443.43 88.45 14 1571 1602 31 444.61 89.02 15 1572 1585 13 445.73 89.39 16 1572 1586 14 445.43 89.29 17 1572 1587 15 444.21 89.31 18 1572 1605 33 445.35 88.82 19 1572 1606 34 445.25 88.86 20 1572 1609 37 465.56 79.51 21 1572 1610 38 465.58 79.51 22 1572 1620 48 445.74 89.40 23 1573 1585 12 446.04 89.25 24 1573 1586 13 445.79 89.24 25 1573 1588 15 445.61 89.33 26 1573 1704 131 333.38 32.92 27 1573 1705 132 333.36 32.92 28 1573 1706 133 332.93 32.98 29 1574 1583 9 562.98 109.88 30 1574 1584 10 543.95 118.72 31 1574 1585 11 446.05 89.25 32 1574 1613 39 484.63 70.55 33 1575 1584 9 561.87 110.42 34 1575 1586 11 542.98 119.21 35 1575 1704 129 333.91 32.65 36 1576 1583 7 563.18 109.78 37 1576 1584 8 562.20 110.26 38 1576 1699 123 380.75 18.55 39 1577 1581 4 802.57 7.75 40 1577 1582 5 715.23 33.76 41 1577 1583 6 563.18 109.78 42 1577 1584 7 563.14 109.79 43 1577 1698 121 381.16 18.75 44 1577 1699 122 381.06 18.63 45 1578 1581 3 827.25 4.59 46 1578 1582 4 782.69 0.06 47 1578 1584 6 715.23 33.76 48 1578 1619 41 637.01 5.38 49 1578 1697 119 381.76 18.52 50 1578 1698 120 381.16 18.75 51 1578 1699 121 381.67 18.49 52 1579 1696 117 381.84 18.51 53 1579 1697 118 381.83 18.50 54 1579 1698 119 381.81 18.50 55 1579 1700 121 381.80 18.48 56 1580 1619 39 766.97 0.26 57 1582 1617 35 779.09 0.48 58 1582 1618 36 779.07 0.48 59 1582 1619 37 779.06 0.48 60 1582 1654 72 598.31 23.15 61 1583 1613 30 779.19 0.50 62 1583 1617 34 779.12 0.48 63 1583 1618 35 779.10 0.48 64 1583 1619 36 779.09 0.48 65 1584 1619 35 779.10 0.48 66 1584 1621 37 779.09 0.48 67 1584 1698 114 387.58 15.54 68 1585 1609 24 779.28 0.54 69 1585 1610 25 779.25 0.53 70 1585 1613 28 779.29 0.48 71 1585 1618 33 779.18 0.49 72 1585 1619 34 779.13 0.48 73 1585 1620 35 779.10 0.48 74 1585 1621 36 779.10 0.48 75 1586 1621 35 779.10 0.48 76 1586 1694 108 388.12 15.39 77 1586 1698 112 387.93 15.37 78 1586 1703 117 378.00 10.63 79 1586 1704 118 377.97 10.63 80 1586 1705 119 377.78 10.70 81 1587 1592 5 836.10 2.27 82 1587 1602 15 779.39 0.60 83 1587 1621 34 779.13 0.48 84 1587 1622 35 779.10 0.48 85 1587 1623 36 779.10 0.48 86 1587 1633 46 716.19 4.94 87 1588 1591 3 854.94 6.69 88 1588 1592 4 837.12 1.76 89 1588 1623 35 779.10 0.48 90 1588 1706 118 377.79 10.55 91 1589 1592 3 870.26 7.32 92 1589 1623 34 779.13 0.48 93 1589 1624 35 779.11 0.48 94 1589 1627 38 738.21 3.65 95 1589 1631 42 723.77 1.63 96 1590 1623 33 779.16 0.48 97 1590 1625 35 779.10 0.49 98 1590 1626 36 779.08 0.49 99 1590 1627 37 748.98 0.17 100 1590 1631 41 723.85 1.62 101 1590 1706 116 377.89 10.51 102 1590 1708 118 377.61 10.44 103 1590 1709 119 377.39 10.52 104 1590 1763 173 227.02 14.28 105 1591 1597 6 782.01 0.78 106 1591 1706 115 377.87 10.52 107 1592 1597 5 781.22 1.06 108 1592 1599 7 780.06 0.49 109 1592 1602 10 779.70 0.54 110 1592 1631 39 724.55 1.37 111 1592 1632 40 724.55 1.37 112 1592 1633 41 724.54 1.37 113 1592 1634 42 724.54 1.37 114 1592 1637 45 682.13 6.13 115 1593 1596 3 875.67 0.54 116 1593 1597 4 782.38 1.64 117 1593 1630 37 724.63 1.36 118 1593 1631 38 724.56 1.37 119 1594 1629 35 774.16 0.56 120 1594 1630 36 724.63 1.36 121 1594 1631 37 724.63 1.36 122 1594 1632 38 724.60 1.36 123 1594 1634 40 724.54 1.38 124 1598 1601 3 868.21 8.83 125 1598 1602 4 842.06 4.26 126 1599 1602 3 868.23 8.82 127 1599 1603 4 833.14 8.71 128 1599 1637 38 647.86 10.22 129 1600 1603 3 861.23 2.80 130 1600 1604 4 835.14 7.71 131 1601 1604 3 855.82 2.63 132 1601 1605 4 843.88 3.33 133 1602 1605 3 843.82 1.29 134 1602 1606 4 832.33 2.32 135 1602 1633 31 723.31 1.77 136 1602 1637 35 647.87 10.22 137 1602 1641 39 615.20 17.45 138 1603 1606 3 846.50 3.29 139 1603 1607 4 832.33 2.32 140 1603 1637 34 638.07 5.91 141 1603 1640 37 621.30 12.36 142 1603 1641 38 613.13 16.41 143 1603 1644 41 609.82 17.88 144 1604 1607 3 848.49 2.39 145 1604 1608 4 840.28 1.66 146 1605 1608 3 854.71 3.18 147 1605 1609 4 840.29 1.66 148 1606 1609 3 864.97 3.76 149 1606 1610 4 838.50 2.54 150 1606 1641 35 613.10 16.42 151 1606 1645 39 605.93 19.74 152 1607 1610 3 864.97 3.76 153 1607 1611 4 838.50 2.54 154 1607 1614 7 810.35 7.29 155 1607 1644 37 606.00 19.78 156 1607 1645 38 605.90 19.73 157 1608 1611 3 849.80 0.01 158 1608 1612 4 857.12 3.99 159 1609 1613 4 855.74 4.69 160 1610 1613 3 859.77 2.67 161 1610 1614 4 822.10 1.42 162 1610 1618 8 779.47 0.40 163 1610 1620 10 779.35 0.43 164 1611 1614 3 859.76 2.67 165 1613 1616 3 866.66 1.60 166 1614 1618 4 807.95 0.23 167 1614 1645 31 605.75 19.73 168 1614 1646 32 605.69 19.75 169 1614 1648 34 604.98 20.09 170 1614 1649 35 604.88 20.14 171 1614 1652 38 604.57 20.25 172 1615 1648 33 604.98 20.09 173 1615 1652 37 604.90 20.09 174 1616 1652 36 604.64 20.18 175 1617 1652 35 600.29 22.36 176 1618 1649 31 604.87 20.13 177 1618 1652 34 600.29 22.36 178 1618 1653 35 598.62 23.18 179 1618 1654 36 598.37 23.29 180 1619 1654 35 588.20 28.38 181 1619 1655 36 587.50 28.71 182 1619 1657 38 587.22 28.82 183 1620 1653 33 598.25 23.38 184 1620 1655 35 587.50 28.72 185 1620 1656 36 567.49 38.68 186 1620 1657 37 567.48 38.68 187 1621 1657 36 567.32 38.69 188 1621 1694 73 388.06 15.42 189 1621 1705 84 377.80 10.71 190 1622 1656 34 567.54 38.70 191 1622 1657 35 567.40 38.73 192 1622 1658 36 567.29 38.69 193 1622 1659 37 566.98 38.85 194 1623 1659 36 566.98 38.84 195 1623 1705 82 377.95 10.62 196 1623 1706 83 377.94 10.62 197 1623 1709 86 375.45 11.50 198 1624 1627 3 829.21 4.58 199 1624 1658 34 567.43 38.75 200 1624 1659 35 567.32 38.72 201 1624 1660 36 566.94 38.88 202 1624 1661 37 566.48 39.05 203 1624 1665 41 539.88 27.14 204 1625 1659 34 567.41 38.76 205 1625 1660 35 566.64 39.02 206 1625 1709 84 376.48 11.00 207 1625 1712 87 374.62 11.35 208 1625 1761 136 247.22 15.47 209 1626 1631 5 831.18 0.39 210 1626 1662 36 566.53 39.08 211 1626 1761 135 230.69 12.44 212 1626 1763 137 227.02 14.27 213 1626 1766 140 211.75 15.39 214 1627 1631 4 813.24 1.80 215 1627 1662 35 566.02 39.32 216 1627 1664 37 563.00 38.70 217 1627 1665 38 554.04 34.26 218 1628 1631 3 844.40 1.81 219 1628 1632 4 832.81 0.43 220 1628 1664 36 563.76 38.34 221 1629 1632 3 835.53 2.92 222 1629 1664 35 565.57 39.24 223 1632 1664 32 564.77 39.59 224 1632 1668 36 531.65 24.07 225 1633 1637 4 820.19 4.98 226 1633 1638 5 800.65 4.59 227 1633 1641 8 799.21 5.32 228 1633 1668 35 531.66 24.07 229 1634 1637 3 850.05 7.55 230 1634 1638 4 829.96 0.10 231 1635 1638 3 844.10 2.00 232 1635 1639 4 844.12 2.01 233 1635 1672 37 452.55 1.46 234 1635 1675 40 447.47 4.00 235 1636 1639 3 860.14 5.34 236 1636 1640 4 844.52 1.84 237 1637 1640 3 848.77 0.28 238 1637 1641 4 844.54 1.84 239 1638 1641 3 848.78 0.28 240 1638 1642 4 848.78 0.27 241 1638 1668 30 531.67 24.07 242 1638 1672 34 452.54 1.46 243 1638 1675 37 442.83 5.58 244 1638 1676 38 442.84 5.58 245 1639 1642 3 863.04 0.93 246 1639 1643 4 844.53 1.85 247 1639 1675 36 440.95 6.52 248 1640 1643 3 851.51 0.97 249 1640 1644 4 839.40 4.28 250 1641 1645 4 843.62 2.16 251 1641 1650 9 804.93 2.48 252 1642 1650 8 804.72 2.58 253 1642 1675 33 440.95 6.52 254 1642 1676 34 440.93 6.53 255 1642 1679 37 438.73 6.79 256 1643 1650 7 839.00 0.01 257 1644 1650 6 842.91 1.81 258 1645 1649 4 848.96 4.27 259 1645 1650 5 839.18 0.04 260 1646 1649 3 853.29 2.10 261 1646 1650 4 853.29 2.10 262 1647 1650 3 861.26 1.86 263 1647 1651 4 848.95 4.26 264 1648 1651 3 855.94 4.53 265 1648 1652 4 843.08 1.73 266 1649 1652 3 861.28 1.85 267 1649 1653 4 855.24 4.88 268 1650 1653 3 865.87 4.29 269 1650 1684 34 427.45 1.41 270 1651 1684 33 427.45 1.41 271 1653 1656 3 916.58 6.22 272 1653 1684 31 427.41 1.43 273 1655 1684 29 427.16 1.49 274 1656 1684 28 427.34 1.40 275 1656 1685 29 427.00 1.54 276 1656 1686 30 426.88 1.48 277 1657 1686 29 426.85 1.50 278 1657 1688 31 426.23 1.46 279 1657 1694 37 388.07 15.43 280 1657 1697 40 388.04 15.42 281 1658 1661 3 897.62 1.36 282 1658 1685 27 427.21 1.31 283 1658 1686 28 427.15 1.34 284 1658 1687 29 426.38 1.40 285 1658 1688 30 426.39 1.39 286 1659 1688 29 426.39 1.39 287 1659 1689 30 426.38 1.38 288 1659 1690 31 426.19 1.44 289 1659 1691 32 425.93 1.36 290 1659 1705 46 378.11 10.55 291 1659 1709 50 376.66 11.12 292 1659 1713 54 372.93 12.28 293 1660 1688 28 426.78 1.20 294 1660 1759 99 257.19 13.15 295 1660 1761 101 251.31 14.52 296 1661 1665 4 838.14 0.89 297 1661 1687 26 426.88 1.15 298 1661 1751 90 292.74 5.25 299 1661 1758 97 262.19 14.54 300 1661 1759 98 261.81 14.73 301 1662 1665 3 863.38 1.23 302 1662 1666 4 836.26 0.07 303 1662 1758 96 261.98 14.70 304 1662 1759 97 261.91 14.69 305 1662 1760 98 260.94 15.04 306 1663 1666 3 839.88 0.01 307 1663 1667 4 836.26 0.07 308 1663 1758 95 264.29 15.59 309 1663 1760 97 261.53 14.88 310 1664 1667 3 840.51 2.19 311 1664 1668 4 836.26 0.08 312 1665 1668 3 837.98 0.94 313 1665 1669 4 836.18 0.09 314 1666 1669 3 841.14 2.56 315 1666 1670 4 837.57 0.78 316 1666 1749 83 292.85 5.29 317 1666 1750 84 292.86 5.29 318 1666 1751 85 292.86 5.29 319 1666 1758 92 267.31 17.06 320 1667 1670 3 848.23 2.52 321 1667 1671 4 846.18 3.53 322 1668 1671 3 846.19 3.53 323 1668 1672 4 846.18 3.53 324 1668 1676 8 640.24 4.17 325 1669 1672 3 846.45 3.40 326 1669 1673 4 817.85 2.74 327 1669 1676 7 534.39 47.04 328 1669 1751 82 292.89 5.27 329 1669 1752 83 292.93 5.26 330 1670 1673 3 837.67 7.79 331 1672 1675 3 853.41 2.16 332 1672 1676 4 826.34 0.04 333 1673 1676 3 841.09 1.03 334 1673 1677 4 810.91 7.75 335 1673 1680 7 443.44 4.77 336 1673 1752 79 292.94 5.25 337 1674 1677 3 847.35 0.24 338 1676 1679 3 859.01 6.07 339 1676 1680 4 835.79 4.67 340 1676 1752 76 292.76 5.26 341 1677 1680 3 851.13 3.00 342 1679 1682 3 849.94 9.66 343 1679 1685 6 475.44 23.12 344 1679 1752 73 292.60 5.32 345 1680 1752 72 292.60 5.32 346 1680 1753 73 292.58 5.32 347 1681 1752 71 292.62 5.31 348 1681 1753 72 292.33 5.27 349 1682 1685 3 849.00 0.08 350 1682 1754 72 292.18 5.33 351 1685 1752 67 292.28 5.23 352 1685 1754 69 290.69 6.00 353 1687 1751 64 292.37 5.33 354 1687 1752 65 292.60 5.33 355 1687 1754 67 290.28 6.25 356 1687 1756 69 267.63 11.95 357 1688 1693 5 518.46 36.99 358 1688 1694 6 429.27 0.51 359 1689 1757 68 265.04 13.13 360 1690 1693 3 859.19 4.75 361 1690 1694 4 851.82 1.07 362 1691 1694 3 848.66 0.52 363 1691 1695 4 845.80 1.94 364 1691 1703 12 378.23 10.55 365 1691 1705 14 378.17 10.55 366 1691 1710 19 375.60 11.68 367 1691 1713 22 356.31 20.57 368 1692 1695 3 848.68 0.52 369 1692 1696 4 851.38 1.86 370 1693 1696 3 858.70 1.79 371 1693 1697 4 856.37 0.62 372 1694 1697 3 863.84 0.78 373 1694 1698 4 848.40 0.35 374 1694 1703 9 378.22 10.55 375 1694 1705 11 378.16 10.56 376 1695 1698 3 855.78 0.92 377 1695 1701 6 382.37 12.52 378 1695 1703 8 378.23 10.55 379 1696 1701 5 493.78 67.94 380 1698 1701 3 847.02 3.46 381 1698 1702 4 814.69 3.81 382 1698 1703 5 407.03 24.69 383 1699 1702 3 857.30 0.28 384 1703 1710 7 800.85 0.47 385 1705 1709 4 835.10 4.44 386 1705 1710 5 831.81 2.79 387 1706 1709 3 837.03 3.47 388 1706 1710 4 840.62 1.67 389 1707 1710 3 846.72 0.59 390 1707 1711 4 840.62 1.68 391 1708 1711 3 842.32 0.94 392 1708 1712 4 846.48 4.20 393 1709 1712 3 854.23 0.33 394 1710 1713 3 851.50 1.69 395 1711 1714 3 876.16 3.14 396 1712 1716 4 874.94 2.23 397 1712 1739 27 278.03 25.95 398 1712 1740 28 271.41 22.67 399 1712 1742 30 268.33 18.07 400 1712 1761 49 257.06 13.21 401 1712 1763 51 248.25 15.98 402 1713 1716 3 902.63 2.10 403 1715 1737 22 285.69 26.72 404 1716 1735 19 286.11 26.50 405 1716 1736 20 285.84 26.64 406 1716 1737 21 285.69 26.72 407 1716 1742 26 290.07 24.53 408 1717 1735 18 291.48 23.84 409 1717 1736 19 286.02 26.56 410 1717 1802 85 83.80 4.82 411 1718 1733 15 307.98 15.60 412 1718 1734 16 296.87 21.15 413 1718 1735 17 293.50 22.84 414 1719 1733 14 307.98 15.61 415 1719 1734 15 297.74 20.73 416 1719 1736 17 297.91 20.84 417 1719 1793 74 146.89 6.03 418 1719 1795 76 113.79 0.33 419 1719 1798 79 99.66 1.73 420 1719 1799 80 88.38 7.04 421 1720 1733 13 307.98 15.61 422 1720 1793 73 150.77 5.88 423 1721 1793 72 150.83 5.86 424 1722 1731 9 312.07 14.72 425 1722 1732 10 311.54 14.59 426 1722 1733 11 311.50 14.61 427 1722 1734 12 311.50 14.61 428 1722 1792 70 152.02 6.38 429 1722 1793 71 145.97 7.06 430 1723 1729 6 312.42 14.75 431 1723 1730 7 312.36 14.77 432 1723 1731 8 312.07 14.72 433 1723 1732 9 312.06 14.73 434 1723 1791 68 152.69 6.37 435 1723 1792 69 152.00 6.38 436 1724 1729 5 312.44 14.75 437 1724 1730 6 312.39 14.75 438 1724 1732 8 312.12 14.76 439 1724 1789 65 153.85 6.17 440 1724 1790 66 153.81 6.16 441 1724 1791 67 153.28 6.35 442 1725 1729 4 437.80 47.93 443 1725 1777 52 154.31 5.94 444 1725 1790 65 153.80 6.17 445 1725 1791 66 153.75 6.18 446 1725 1792 67 153.75 6.18 447 1726 1729 3 822.28 0.70 448 1730 1745 15 436.43 48.38 449 1730 1747 17 436.32 48.38 450 1731 1745 14 436.44 48.40 451 1731 1746 15 436.31 48.41 452 1731 1747 16 436.28 48.43 453 1732 1743 11 436.54 48.40 454 1732 1744 12 436.48 48.40 455 1732 1745 13 436.38 48.41 456 1732 1746 14 436.29 48.43 457 1732 1791 59 152.36 6.33 458 1733 1743 10 436.70 48.44 459 1733 1744 11 436.48 48.40 460 1734 1741 7 436.94 48.39 461 1734 1742 8 436.89 48.37 462 1734 1743 9 436.70 48.44 463 1734 1778 44 153.39 6.40 464 1734 1791 57 152.79 6.52 465 1734 1799 65 87.89 6.79 466 1735 1741 6 436.97 48.40 467 1735 1742 7 436.89 48.38 468 1735 1744 9 436.89 48.37 469 1736 1740 4 547.11 8.27 470 1736 1741 5 436.97 48.41 471 1736 1799 63 87.89 6.79 472 1736 1802 66 87.80 6.82 473 1736 1803 67 87.80 6.82 474 1736 1806 70 87.12 7.14 475 1737 1740 3 832.59 2.85 476 1737 1806 69 86.96 7.24 477 1738 1806 68 86.95 7.24 478 1738 1809 71 82.44 7.26 479 1739 1763 24 412.81 59.95 480 1739 1764 25 412.73 59.97 481 1739 1806 67 85.67 7.90 482 1740 1761 21 417.38 57.86 483 1740 1762 22 414.33 59.27 484 1740 1763 23 412.76 59.96 485 1740 1764 24 412.69 59.94 486 1741 1761 20 417.39 57.75 487 1741 1762 21 414.22 59.31 488 1741 1767 26 360.68 59.97 489 1741 1780 39 153.75 6.22 490 1741 1803 62 87.81 6.83 491 1742 1759 17 417.83 57.64 492 1742 1760 18 417.47 57.77 493 1742 1761 19 416.63 58.11 494 1743 1759 16 417.80 57.62 495 1743 1760 17 417.64 57.62 496 1743 1762 19 417.38 57.73 497 1743 1791 48 152.25 6.27 498 1744 1758 14 418.12 57.48 499 1744 1759 15 417.66 57.59 500 1745 1757 12 418.14 57.47 501 1745 1758 13 418.06 57.50 502 1746 1757 11 418.67 57.60 503 1747 1757 10 418.91 57.57 504 1748 1755 7 419.15 57.52 505 1748 1756 8 419.04 57.51 506 1748 1757 9 418.91 57.57 507 1749 1754 5 670.98 8.49 508 1749 1755 6 419.20 57.51 509 1749 1756 7 419.18 57.51 510 1749 1758 9 419.14 57.48 511 1750 1753 3 843.15 3.83 512 1750 1754 4 518.08 8.15 513 1750 1755 5 517.99 8.14 514 1751 1754 3 835.93 0.49 515 1751 1756 5 587.27 26.46 516 1751 1758 7 517.96 8.14 517 1762 1766 4 820.78 2.08 518 1762 1767 5 741.83 37.41 519 1762 1782 20 156.44 6.13 520 1762 1785 23 166.85 4.83 521 1762 1789 27 153.91 6.29 522 1763 1766 3 838.31 1.71 523 1763 1767 4 830.75 1.67 524 1764 1767 3 843.59 3.27 525 1764 1806 42 85.68 7.90 526 1764 1807 43 85.56 7.91 527 1764 1810 46 59.52 1.36 528 1765 1807 42 85.69 7.90 529 1765 1810 45 59.49 1.35 530 1766 1782 16 170.09 3.46 531 1766 1783 17 170.08 3.46 532 1766 1807 41 85.70 7.90 533 1767 1770 3 913.87 2.83 534 1767 1780 13 170.15 3.42 535 1767 1781 14 170.17 3.44 536 1767 1782 15 170.12 3.46 537 1767 1803 36 88.16 6.99 538 1767 1807 40 85.67 7.88 539 1768 1781 13 170.22 3.46 540 1768 1783 15 170.22 3.46 541 1769 1780 11 170.30 3.48 542 1769 1781 12 170.28 3.48 543 1770 1779 9 170.41 3.52 544 1770 1780 10 170.31 3.48 545 1770 1800 30 88.42 7.05 546 1770 1803 33 88.41 7.05 547 1770 1804 34 88.28 7.04 548 1770 1807 37 87.89 6.92 549 1771 1778 7 170.47 3.50 550 1771 1779 8 170.41 3.52 551 1772 1777 5 234.25 35.33 552 1772 1778 6 170.49 3.50 553 1772 1796 24 137.04 8.09 554 1772 1797 25 118.87 0.98 555 1772 1800 28 104.77 0.22 556 1773 1777 4 234.27 35.33 557 1773 1778 5 170.56 3.49 558 1773 1779 6 170.56 3.50 559 1774 1777 3 814.51 5.23 560 1775 1796 21 165.34 2.39 561 1775 1797 22 147.83 6.13 562 1776 1792 16 170.20 3.58 563 1776 1793 17 170.16 3.58 564 1776 1794 18 169.94 3.56 565 1776 1796 20 147.83 6.13 566 1777 1790 13 170.30 3.58 567 1777 1791 14 170.20 3.58 568 1777 1792 15 170.12 3.62 569 1777 1796 19 136.47 6.94 570 1778 1790 12 170.25 3.60 571 1778 1791 13 170.19 3.58 572 1778 1796 18 126.69 2.92 573 1778 1799 21 109.17 0.86 574 1778 1800 22 103.90 0.21 575 1778 1803 25 94.73 4.25 576 1779 1788 9 170.46 3.65 577 1779 1789 10 170.33 3.64 578 1779 1790 11 170.16 3.62 579 1780 1788 8 170.40 3.68 580 1780 1789 9 170.35 3.70 581 1780 1791 11 170.24 3.67 582 1780 1803 23 88.36 7.05 583 1781 1788 7 170.34 3.72 584 1782 1785 3 864.89 7.17 585 1782 1789 7 170.34 3.73 586 1792 1796 4 829.15 2.09 587 1793 1796 3 853.46 1.17 588 1793 1799 6 742.54 36.83 589 1795 1798 3 855.23 2.67 590 1795 1799 4 841.62 4.13 591 1796 1799 3 843.55 3.18 592 1796 1800 4 841.62 4.13 593 1797 1800 3 853.89 0.93 594 1797 1801 4 830.99 1.21 595 1798 1801 3 841.65 4.11 596 1798 1802 4 831.02 1.22 597 1799 1802 3 848.65 2.96 598 1799 1803 4 830.18 1.65 599 1800 1803 3 845.83 2.17 600 1800 1804 4 839.43 2.98 601 1801 1804 3 840.61 2.39 602 1801 1805 4 831.22 1.31 603 1802 1805 3 850.03 2.35 604 1802 1806 4 835.36 0.81 605 1803 1806 3 842.25 2.64 606 1803 1807 4 835.35 0.81 607 1804 1807 3 839.43 2.95 608 1804 1808 4 833.62 0.05 609 1805 1808 3 848.97 0.72 610 1805 1809 4 811.33 3.33 611 1806 1809 3 865.36 10.47 612 1806 1810 4 811.01 3.50 613 1807 1810 3 849.71 2.64 614 1807 1811 4 829.52 1.82 615 1808 1811 3 843.62 3.04 616 1808 1812 4 831.73 2.93 617 1809 1812 3 856.44 3.36 618 1809 1813 4 855.39 2.84 619 1810 1813 3 856.95 2.33 620 1810 1814 4 843.64 3.03 621 1811 1814 3 843.65 3.03