BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 93 91 42.51 0.57 2 2 128 126 42.45 0.55 3 3 90 87 42.51 0.57 4 3 93 90 42.51 0.57 5 3 94 91 44.46 0.19 6 3 95 92 48.78 0.45 7 3 125 122 42.51 0.57 8 3 126 123 42.77 0.45 9 3 127 124 42.76 0.45 10 3 128 125 42.51 0.57 11 4 9 5 793.53 1.80 12 4 93 89 47.75 0.45 13 4 94 90 47.83 0.62 14 4 95 91 51.55 1.27 15 4 209 205 61.73 0.42 16 4 210 206 61.50 0.34 17 4 212 208 60.59 0.01 18 5 8 3 813.52 0.59 19 5 9 4 797.50 0.18 20 5 95 90 52.38 1.68 21 6 9 3 808.60 5.37 22 6 10 4 808.60 5.37 23 6 36 30 61.09 0.26 24 6 38 32 59.56 0.32 25 6 94 88 52.38 1.68 26 6 95 89 52.43 1.70 27 6 96 90 52.44 1.70 28 6 174 168 60.99 0.16 29 6 200 194 63.55 0.95 30 6 204 198 65.52 0.04 31 6 212 206 65.47 0.01 32 7 10 3 807.26 3.73 33 7 11 4 793.53 1.80 34 7 29 22 76.41 0.03 35 7 30 23 71.42 0.18 36 7 36 29 66.22 0.18 37 7 96 89 52.44 1.70 38 8 11 3 798.48 0.68 39 8 12 4 793.50 1.81 40 9 12 3 801.03 3.00 41 9 13 4 793.24 2.09 42 9 203 194 80.26 0.95 43 9 204 195 80.23 0.95 44 9 207 198 80.28 0.94 45 9 209 200 80.16 0.90 46 10 13 3 806.37 8.71 47 10 14 4 793.18 2.12 48 10 28 18 80.89 0.81 49 10 29 19 80.83 0.78 50 10 36 26 80.20 0.95 51 10 178 168 80.69 0.80 52 10 179 169 80.83 0.78 53 10 180 170 80.92 0.76 54 10 198 188 80.77 0.77 55 10 203 193 80.33 0.92 56 11 14 3 808.56 5.40 57 11 15 4 792.97 2.23 58 11 25 14 594.78 20.87 59 11 28 17 80.88 0.82 60 12 15 3 812.25 5.80 61 12 16 4 793.12 2.32 62 12 207 195 80.47 0.83 63 13 16 3 817.83 3.27 64 13 17 4 793.11 2.33 65 13 180 167 81.29 0.88 66 13 198 185 81.09 0.82 67 13 203 190 80.25 0.94 68 13 206 193 80.28 0.95 69 13 207 194 80.25 0.94 70 14 17 3 801.47 2.44 71 14 18 4 795.59 1.09 72 14 25 11 597.25 22.10 73 14 180 166 81.23 0.89 74 14 182 168 83.52 0.79 75 15 18 3 798.60 1.00 76 15 19 4 798.60 1.00 77 15 25 10 662.14 12.81 78 16 19 3 809.58 4.49 79 16 20 4 794.01 3.29 80 17 20 3 797.06 1.77 81 17 21 4 788.50 0.60 82 17 23 6 786.24 0.38 83 17 182 165 96.98 0.12 84 17 196 179 87.12 1.25 85 18 21 3 797.99 0.28 86 18 22 4 786.55 0.36 87 18 23 5 759.74 0.34 88 18 24 6 752.49 3.09 89 18 25 7 694.53 2.32 90 18 182 164 96.98 0.12 91 19 22 3 788.11 0.42 92 23 182 159 165.22 0.04 93 23 183 160 167.07 0.45 94 23 184 161 167.15 0.43 95 23 194 171 166.84 0.45 96 24 182 158 167.13 0.42 97 24 183 159 167.15 0.43 98 25 180 155 167.16 0.43 99 25 182 157 167.18 0.43 100 26 181 155 167.19 0.43 101 26 182 156 167.20 0.43 102 26 183 157 167.26 0.44 103 27 180 153 167.22 0.45 104 27 181 154 167.22 0.45 105 28 179 151 167.22 0.45 106 28 180 152 167.23 0.45 107 29 178 149 167.22 0.45 108 29 179 150 167.25 0.45 109 29 181 152 201.46 1.35 110 30 35 5 704.13 7.18 111 30 36 6 655.73 5.25 112 30 177 147 167.24 0.44 113 30 178 148 167.24 0.44 114 31 34 3 808.76 0.43 115 31 35 4 713.68 8.17 116 31 177 146 167.25 0.45 117 31 178 147 167.35 0.44 118 31 179 148 187.66 0.14 119 31 197 166 195.47 0.17 120 32 35 3 800.30 3.43 121 34 176 142 195.56 0.74 122 34 177 143 211.94 0.04 123 35 176 141 213.18 0.66 124 36 96 60 185.33 1.48 125 36 175 139 214.40 0.06 126 36 176 140 214.53 0.04 127 36 177 141 214.83 0.16 128 36 178 142 214.90 0.18 129 36 200 164 214.36 0.29 130 37 174 137 214.40 0.07 131 37 175 138 214.72 0.11 132 38 41 3 896.01 2.92 133 38 94 56 196.12 0.53 134 38 95 57 196.16 0.53 135 38 96 58 205.33 3.27 136 38 100 62 189.68 0.69 137 38 125 87 189.41 0.50 138 38 126 88 192.46 0.53 139 38 172 134 208.12 1.02 140 38 173 135 214.58 0.07 141 38 174 136 214.70 0.12 142 39 172 133 214.59 0.08 143 39 173 134 214.72 0.12 144 40 172 132 214.97 0.15 145 41 100 59 208.75 1.83 146 41 102 61 196.09 0.53 147 41 104 63 193.39 1.15 148 41 125 84 207.11 2.10 149 41 171 130 214.99 0.14 150 41 172 131 214.97 0.15 151 42 104 62 195.09 1.02 152 42 170 128 215.00 0.15 153 42 171 129 214.99 0.14 154 43 104 61 198.42 0.16 155 43 121 78 199.79 2.10 156 43 169 126 215.01 0.15 157 43 170 127 215.01 0.15 158 44 168 124 215.01 0.15 159 44 169 125 215.05 0.14 160 44 171 127 217.36 0.32 161 45 48 3 827.81 6.62 162 45 106 61 214.56 0.22 163 45 108 63 214.71 0.12 164 45 119 74 214.62 0.19 165 45 166 121 214.96 0.14 166 45 167 122 215.04 0.14 167 45 168 123 215.05 0.14 168 45 229 184 215.00 0.16 169 46 167 121 215.32 0.21 170 46 168 122 216.01 0.13 171 46 169 123 221.50 2.36 172 47 166 119 215.31 0.21 173 48 107 59 215.19 0.14 174 48 108 60 215.08 0.16 175 48 109 61 215.18 0.12 176 49 109 60 215.59 0.34 177 49 111 62 215.83 0.43 178 49 166 117 241.50 1.55 179 50 108 58 218.23 0.68 180 50 109 59 215.96 0.26 181 50 110 60 217.28 1.16 182 50 111 61 218.24 0.68 183 50 163 113 217.99 0.88 184 50 164 114 218.00 0.87 185 50 166 116 241.53 1.56 186 51 111 60 233.96 2.19 187 51 112 61 238.22 2.44 188 52 112 60 241.44 1.60 189 52 163 111 241.43 1.57 190 52 164 112 241.51 1.56 191 53 112 59 241.44 1.60 192 54 113 59 241.48 1.61 193 54 160 106 238.20 2.11 194 54 161 107 241.37 1.56 195 54 162 108 241.44 1.57 196 54 235 181 241.39 1.57 197 55 159 104 241.48 1.57 198 55 160 105 241.49 1.57 199 55 161 106 241.49 1.57 200 56 158 102 237.78 0.69 201 56 159 103 241.48 1.57 202 56 160 104 241.49 1.57 203 56 238 182 240.66 1.22 204 57 82 25 241.75 1.58 205 57 158 101 241.48 1.57 206 57 159 102 241.50 1.57 207 57 161 104 241.72 1.57 208 58 156 98 240.40 1.57 209 58 157 99 241.49 1.56 210 58 158 100 241.53 1.57 211 59 80 21 241.73 1.56 212 59 82 23 241.83 1.59 213 59 139 80 241.71 1.58 214 59 157 98 241.57 1.56 215 59 159 100 241.73 1.57 216 59 237 178 241.72 1.56 217 60 78 18 241.69 1.55 218 60 80 20 241.82 1.58 219 60 156 96 241.69 1.55 220 60 157 97 241.63 1.58 221 61 154 93 415.95 0.91 222 61 155 94 411.91 0.61 223 61 156 95 241.69 1.55 224 62 76 14 415.44 0.69 225 62 78 16 415.55 0.76 226 62 154 92 416.75 0.56 227 62 155 93 415.84 0.85 228 63 76 13 418.22 0.62 229 63 152 89 419.00 0.82 230 63 153 90 418.44 0.58 231 63 154 91 416.75 0.56 232 64 72 8 664.07 2.40 233 64 74 10 418.74 0.72 234 64 75 11 418.76 0.72 235 64 76 12 418.53 0.61 236 64 147 83 448.01 3.24 237 64 152 88 419.01 0.82 238 64 153 89 418.67 0.69 239 65 151 86 464.38 0.96 240 65 152 87 419.00 0.81 241 65 246 181 241.44 1.58 242 66 70 4 812.81 2.37 243 66 71 5 702.42 3.91 244 66 72 6 674.94 1.65 245 66 147 81 480.09 6.07 246 66 148 82 492.49 0.32 247 66 149 83 492.92 0.53 248 66 150 84 484.21 4.46 249 66 151 85 479.88 6.18 250 66 246 180 241.56 1.57 251 67 150 83 491.92 0.61 252 67 246 179 241.56 1.57 253 68 149 81 512.51 1.59 254 68 150 82 511.18 2.26 255 69 149 80 516.25 0.09 256 69 150 81 516.25 0.09 257 70 149 79 516.30 0.07 258 71 146 75 524.25 0.61 259 71 147 76 523.54 0.52 260 71 148 77 521.20 1.58 261 71 149 78 520.25 2.02 262 72 146 74 524.25 0.61 263 72 147 75 524.09 0.66 264 73 144 71 524.67 0.69 265 73 145 72 524.46 0.61 266 73 146 73 524.27 0.62 267 74 144 70 524.69 0.69 268 74 145 71 524.47 0.61 269 75 142 67 525.16 0.56 270 75 143 68 525.03 0.57 271 75 144 69 524.69 0.69 272 76 142 66 525.17 0.57 273 76 143 67 525.09 0.58 274 76 145 69 525.02 0.57 275 76 153 77 524.24 0.59 276 76 154 78 524.20 0.58 277 76 155 79 521.29 1.50 278 76 241 165 242.33 1.79 279 77 141 64 525.41 0.48 280 77 142 65 525.16 0.56 281 78 140 62 525.50 0.48 282 78 141 63 525.44 0.47 283 78 155 77 524.24 0.59 284 78 157 79 523.14 0.52 285 78 239 161 242.69 1.85 286 79 139 60 525.69 0.53 287 79 140 61 525.51 0.48 288 80 138 58 526.12 0.61 289 80 139 59 525.95 0.67 290 80 157 77 524.20 0.58 291 81 136 55 526.44 0.57 292 81 137 56 526.35 0.55 293 81 138 57 526.12 0.61 294 82 136 54 526.51 0.57 295 82 137 55 526.44 0.57 296 82 139 57 526.24 0.57 297 82 159 77 524.15 0.56 298 82 161 79 523.36 0.55 299 83 134 51 527.17 0.46 300 83 135 52 527.10 0.47 301 83 136 53 526.71 0.47 302 84 134 50 527.39 0.36 303 84 135 51 527.16 0.46 304 84 161 77 523.79 0.38 305 84 232 148 287.97 6.84 306 84 233 149 280.42 9.29 307 85 132 47 546.15 0.21 308 85 133 48 542.63 0.35 309 85 134 49 534.92 3.40 310 85 231 146 292.31 6.50 311 85 232 147 289.89 6.16 312 86 120 34 547.02 0.24 313 86 121 35 546.39 0.04 314 86 132 46 546.33 0.14 315 86 133 47 543.54 0.58 316 86 164 78 522.39 0.55 317 86 230 144 305.65 5.80 318 86 231 145 292.31 6.50 319 87 130 43 547.96 0.53 320 87 131 44 547.22 0.17 321 87 132 45 546.45 0.09 322 87 229 142 311.61 7.65 323 87 230 143 306.12 5.74 324 88 121 33 547.29 0.21 325 88 122 34 547.31 0.20 326 88 123 35 547.31 0.20 327 88 130 42 547.99 0.54 328 88 131 43 547.86 0.48 329 88 228 140 313.25 7.88 330 88 229 141 312.16 7.90 331 89 128 39 548.32 0.49 332 89 129 40 548.29 0.48 333 89 130 41 548.03 0.52 334 89 227 138 313.99 7.62 335 89 228 139 313.93 7.64 336 90 93 3 830.87 1.99 337 90 124 34 548.34 0.51 338 90 125 35 548.34 0.51 339 90 127 37 548.47 0.47 340 90 128 38 548.34 0.51 341 90 170 80 497.15 2.12 342 90 226 136 315.30 7.15 343 90 227 137 313.99 7.62 344 91 226 135 315.29 7.14 345 91 227 136 314.95 7.28 346 92 223 131 321.13 6.38 347 92 225 133 315.60 7.02 348 92 226 134 315.58 7.00 349 93 125 32 548.48 0.48 350 93 223 130 321.11 6.38 351 94 125 31 554.41 0.83 352 94 126 32 554.43 0.83 353 94 172 78 497.04 2.05 354 94 212 118 321.42 6.54 355 94 223 129 321.12 6.38 356 95 98 3 839.71 1.97 357 95 126 31 554.44 0.83 358 98 126 28 625.33 5.98 359 99 126 27 625.34 5.98 360 99 127 28 625.33 5.98 361 100 124 24 625.53 6.02 362 100 126 26 625.37 5.99 363 101 122 21 629.74 4.69 364 101 123 22 625.96 6.10 365 101 124 23 625.56 6.00 366 101 131 30 625.28 5.95 367 102 122 20 629.78 4.70 368 102 123 21 629.39 4.82 369 103 120 17 631.54 4.34 370 103 121 18 631.26 4.28 371 103 122 19 630.29 4.51 372 103 133 30 625.22 5.92 373 104 120 16 631.54 4.34 374 104 121 17 631.35 4.30 375 104 122 18 631.13 4.21 376 104 123 19 631.13 4.21 377 105 118 13 632.22 4.28 378 105 119 14 631.88 4.32 379 105 120 15 631.51 4.36 380 105 121 16 631.51 4.36 381 106 118 12 632.35 4.22 382 106 119 13 632.15 4.25 383 107 117 10 648.51 3.49 384 107 118 11 632.36 4.22 385 108 116 8 649.89 2.86 386 108 117 9 648.58 3.52 387 108 119 11 648.50 3.50 388 108 164 56 524.26 0.45 389 109 114 5 713.10 7.53 390 109 115 6 678.52 2.89 391 109 116 7 649.91 2.85 392 110 113 3 813.77 2.63 393 110 114 4 732.87 2.35 394 110 115 5 678.55 2.90 395 110 117 7 678.46 2.93 396 110 162 52 524.54 0.39 397 110 163 53 524.53 0.39 398 110 164 54 524.46 0.42 399 110 233 123 304.61 12.44 400 110 235 125 304.01 12.64 401 111 114 3 791.74 4.44 402 113 160 47 525.37 0.48 403 113 235 122 304.23 12.63 404 115 235 120 304.16 12.60 405 115 236 121 304.08 12.62 406 117 233 116 304.60 12.37 407 117 235 118 303.28 12.34 408 119 164 45 523.75 0.33 409 119 229 110 313.56 7.99 410 119 233 114 304.57 12.35 411 120 133 13 625.15 6.00 412 121 133 12 625.10 5.99 413 121 229 108 313.14 8.08 414 122 131 9 625.27 5.96 415 122 133 11 625.01 5.94 416 123 131 8 625.24 5.98 417 124 127 3 824.21 2.12 418 124 131 7 630.94 3.13 419 125 170 45 497.00 2.04 420 125 223 98 321.01 6.42 421 127 131 4 886.64 0.07 422 137 234 97 248.41 1.90 423 139 157 18 524.17 0.56 424 139 159 20 522.95 0.69 425 139 234 95 248.42 1.90 426 139 237 98 243.31 2.00 427 140 157 17 524.13 0.55 428 141 157 16 523.68 0.68 429 141 237 96 242.97 1.97 430 141 239 98 242.67 1.83 431 142 239 97 242.62 1.84 432 143 239 96 242.41 1.75 433 143 241 98 242.36 1.77 434 145 153 8 593.86 32.71 435 145 241 96 242.12 1.67 436 145 243 98 242.05 1.66 437 146 243 97 241.98 1.63 438 147 151 4 749.88 2.33 439 147 152 5 703.18 1.11 440 147 153 6 643.88 7.71 441 148 151 3 798.29 2.33 442 148 243 95 241.86 1.56 443 150 245 95 241.63 1.56 444 150 246 96 241.58 1.57 445 150 249 99 148.09 0.09 446 151 243 92 241.74 1.55 447 151 244 93 241.62 1.56 448 151 245 94 241.57 1.57 449 151 246 95 241.56 1.57 450 151 250 99 105.82 6.04 451 152 243 91 241.75 1.64 452 152 244 92 241.59 1.56 453 152 246 94 241.20 1.48 454 152 247 95 241.56 1.57 455 153 241 88 242.22 1.78 456 153 242 89 242.15 1.78 457 153 243 90 241.75 1.65 458 154 240 86 242.33 1.82 459 154 241 87 242.13 1.82 460 154 242 88 242.11 1.82 461 155 240 85 242.32 1.83 462 155 241 86 242.12 1.83 463 156 238 82 242.88 1.98 464 156 239 83 242.43 1.84 465 156 240 84 242.38 1.82 466 157 237 80 243.03 2.00 467 157 238 81 242.84 1.95 468 157 239 82 242.50 1.87 469 158 237 79 243.03 2.00 470 158 238 80 242.97 2.01 471 159 234 75 249.94 2.65 472 159 235 76 248.34 1.91 473 159 236 77 246.53 2.33 474 159 237 78 243.18 2.02 475 160 234 74 258.46 1.03 476 160 235 75 248.34 1.91 477 160 236 76 248.25 1.94 478 160 238 78 245.89 2.07 479 161 232 71 283.33 9.37 480 161 233 72 274.24 6.79 481 161 234 73 258.50 1.01 482 162 232 70 288.19 6.96 483 162 233 71 283.24 9.40 484 162 235 73 274.22 6.79 485 163 231 68 291.44 5.82 486 163 232 69 289.44 6.33 487 164 229 65 308.98 6.30 488 164 230 66 295.00 7.35 489 164 231 67 291.52 5.81 490 164 232 68 291.50 5.80 491 164 233 69 291.50 5.80 492 165 229 64 308.98 6.30 493 165 230 65 302.20 3.75 494 166 228 62 313.92 7.55 495 166 229 63 312.14 7.59 496 167 226 59 315.57 6.97 497 167 227 60 315.37 6.92 498 167 228 61 313.95 7.57 499 168 226 58 315.59 6.96 500 168 227 59 315.39 6.94 501 168 228 60 314.31 7.44 502 168 229 61 314.04 7.58 503 169 224 55 316.04 6.86 504 169 225 56 315.81 6.94 505 169 226 57 315.59 6.96 506 170 224 54 316.05 6.86 507 170 225 55 315.81 6.95 508 170 226 56 315.79 6.95 509 170 227 57 315.77 6.95 510 171 223 52 320.20 6.73 511 171 224 53 317.33 6.24 512 172 222 50 322.78 6.08 513 172 223 51 320.98 6.35 514 173 220 47 323.45 6.21 515 173 221 48 322.97 6.15 516 173 222 49 322.88 6.14 517 174 200 26 324.34 5.83 518 174 204 30 323.97 5.97 519 174 212 38 323.31 6.21 520 174 220 46 323.47 6.21 521 174 221 47 323.22 6.25 522 174 223 49 322.95 6.15 523 175 200 25 324.34 5.83 524 175 219 44 323.63 6.25 525 175 220 45 323.47 6.21 526 176 199 23 324.85 5.93 527 176 200 24 324.62 5.86 528 177 197 20 327.16 5.36 529 177 198 21 326.84 5.48 530 177 199 22 324.93 5.94 531 178 197 19 345.44 2.74 532 178 198 20 326.91 5.46 533 179 195 16 365.25 10.64 534 179 196 17 353.28 4.71 535 179 197 18 345.36 2.69 536 180 195 15 365.26 10.64 537 180 196 16 365.19 10.67 538 180 198 18 353.25 4.72 539 181 193 12 366.85 10.71 540 181 194 13 366.79 10.72 541 181 195 14 365.50 10.54 542 182 193 11 366.86 10.71 543 182 194 12 366.81 10.73 544 182 196 14 366.79 10.73 545 183 191 8 647.75 10.93 546 183 192 9 367.77 10.39 547 183 193 10 366.86 10.71 548 183 194 11 366.85 10.71 549 184 191 7 647.75 10.93 550 184 192 8 588.39 16.96 551 184 194 10 367.85 10.24 552 185 190 5 660.74 4.49 553 185 191 6 647.76 10.93 554 186 190 4 663.46 3.14 555 186 191 5 660.74 4.50 556 186 192 6 660.74 4.50 557 187 190 3 816.27 0.27 558 198 203 5 779.84 0.81 559 199 202 3 797.95 0.12 560 199 203 4 789.57 4.06 561 199 219 20 374.52 13.18 562 200 203 3 807.71 4.47 563 200 204 4 789.57 4.06 564 201 204 3 808.93 3.86 565 201 205 4 792.94 2.38 566 201 214 13 374.76 13.18 567 201 216 15 374.89 13.18 568 201 219 18 374.66 13.22 569 201 220 19 374.62 13.23 570 201 221 20 374.43 13.19 571 202 205 3 795.17 1.26 572 202 206 4 792.94 2.38 573 203 206 3 798.49 1.02 574 203 207 4 791.44 3.10 575 204 207 3 805.98 2.68 576 204 208 4 791.44 3.10 577 204 209 5 748.70 1.54 578 204 212 8 554.09 80.34 579 204 214 10 374.99 13.13 580 204 221 17 374.89 13.10 581 205 208 3 791.27 2.41 582 205 214 9 375.06 13.09 583 205 216 11 375.33 13.02 584 209 212 3 851.71 2.54 585 211 223 12 374.97 13.18 586 211 224 13 374.84 13.23 587 212 221 9 376.08 13.21 588 212 222 10 375.81 13.14 589 212 223 11 375.29 13.02 590 213 221 8 510.10 65.07 591 213 222 9 375.89 13.18 592 214 220 6 656.11 2.07 593 214 221 7 599.77 20.25 594 215 218 3 800.09 1.04 595 215 219 4 732.55 0.59 596 215 220 5 712.12 3.78 597 215 222 7 616.21 22.02 598 216 219 3 772.30 4.80 599 218 222 4 893.75 1.90 600 245 249 4 716.06 2.78 601 245 250 5 645.72 4.24 602 246 249 3 825.57 3.90