BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 247 244 31.75 0.03 2 3 248 245 25.78 0.13 3 3 249 246 21.90 0.11 4 3 250 247 19.23 0.67 5 4 248 244 28.31 0.07 6 4 249 245 28.12 0.03 7 5 116 111 44.77 0.85 8 5 240 235 44.94 0.91 9 5 244 239 38.91 2.10 10 5 247 242 33.78 0.01 11 5 248 243 30.32 0.79 12 6 117 111 46.06 0.28 13 6 118 112 42.15 0.41 14 6 247 241 36.86 1.41 15 6 248 242 32.91 0.45 16 8 12 4 849.95 1.18 17 8 13 5 841.59 5.36 18 8 117 109 193.49 72.72 19 8 118 110 44.91 0.92 20 9 12 3 863.40 2.54 21 9 13 4 858.51 0.05 22 10 13 3 871.39 1.47 23 10 14 4 853.01 2.71 24 11 14 3 863.44 2.51 25 11 15 4 862.47 2.02 26 11 90 79 550.96 18.93 27 12 15 3 864.57 0.98 28 12 16 4 864.56 0.98 29 12 90 78 550.97 18.93 30 12 91 79 549.56 18.18 31 12 92 80 549.57 18.18 32 12 95 83 549.56 18.17 33 12 117 105 514.80 0.80 34 13 16 3 864.57 0.98 35 13 17 4 864.86 1.72 36 14 17 3 869.60 4.08 37 14 18 4 864.87 1.72 38 15 18 3 865.51 1.40 39 15 19 4 861.43 3.43 40 15 88 73 563.89 20.68 41 15 90 75 564.44 20.96 42 15 91 76 563.90 20.70 43 16 19 3 870.39 1.04 44 16 20 4 861.43 3.43 45 16 91 75 564.45 20.96 46 16 99 83 563.86 20.66 47 16 115 99 541.60 1.94 48 16 117 101 527.09 2.27 49 17 20 3 876.12 1.82 50 17 21 4 861.74 5.38 51 18 21 3 874.64 1.08 52 18 22 4 861.73 5.38 53 18 88 70 581.12 17.41 54 19 22 3 882.39 2.80 55 19 23 4 847.22 3.37 56 19 88 69 581.11 17.41 57 19 91 72 581.09 17.41 58 19 96 77 580.64 17.20 59 19 99 80 576.55 15.14 60 20 23 3 884.63 6.06 61 20 24 4 857.05 3.56 62 20 99 79 577.16 14.87 63 20 103 83 587.78 9.32 64 20 106 86 587.93 8.75 65 21 24 3 886.96 4.90 66 21 25 4 853.27 5.86 67 22 25 3 869.17 1.69 68 22 26 4 848.02 3.23 69 22 84 62 594.64 12.21 70 22 88 66 594.52 12.14 71 23 26 3 872.73 3.87 72 23 27 4 847.68 8.22 73 23 81 58 594.66 12.21 74 23 84 61 594.65 12.21 75 23 96 73 593.73 11.75 76 23 99 76 593.32 11.57 77 23 100 77 593.01 11.45 78 23 103 80 593.06 11.41 79 24 27 3 893.07 1.62 80 24 28 4 848.85 6.90 81 24 103 79 593.12 11.40 82 24 106 82 592.95 11.04 83 24 107 83 586.23 7.68 84 25 28 3 884.91 0.84 85 25 29 4 864.48 0.94 86 26 29 3 874.79 4.22 87 26 30 4 864.48 0.93 88 26 32 6 841.73 6.17 89 26 80 54 595.33 11.92 90 26 81 55 595.02 12.07 91 26 84 58 595.01 12.07 92 27 30 3 866.81 2.10 93 27 31 4 852.50 5.03 94 27 32 5 810.24 0.66 95 27 33 6 782.01 6.17 96 27 36 9 699.78 9.61 97 27 100 73 594.21 11.52 98 27 103 76 593.94 11.28 99 27 104 77 593.66 11.13 100 27 107 80 588.05 6.83 101 28 31 3 865.94 0.20 102 30 80 50 599.56 9.98 103 31 184 153 248.73 27.66 104 32 35 3 876.52 1.14 105 32 36 4 856.81 3.12 106 32 80 48 612.21 3.79 107 33 36 3 865.94 1.45 108 33 37 4 856.82 3.12 109 33 104 71 607.99 4.44 110 33 107 74 608.26 3.94 111 33 108 75 607.48 3.55 112 33 165 132 253.47 25.50 113 34 37 3 864.53 0.61 114 34 38 4 856.82 3.11 115 34 165 131 253.47 25.50 116 34 166 132 253.50 25.48 117 34 183 149 257.39 23.54 118 34 184 150 254.03 25.13 119 35 38 3 864.78 0.73 120 35 39 4 856.79 3.10 121 35 73 38 637.95 1.25 122 35 74 39 637.93 1.25 123 35 76 41 637.90 1.26 124 35 77 42 637.73 1.27 125 35 80 45 637.77 1.24 126 36 39 3 868.44 7.44 127 36 40 4 848.99 4.36 128 36 77 41 637.88 1.27 129 36 80 44 637.87 1.27 130 36 81 45 637.77 1.24 131 36 100 64 609.99 4.25 132 36 101 65 609.94 4.25 133 36 104 68 608.69 4.12 134 37 40 3 882.91 0.20 135 37 41 4 864.83 9.23 136 37 101 64 609.95 4.25 137 37 104 67 609.07 3.94 138 37 105 68 608.69 4.12 139 37 162 125 260.99 21.99 140 37 165 128 260.88 21.80 141 37 235 198 202.65 0.76 142 37 239 202 196.30 1.46 143 38 41 3 889.28 2.98 144 38 42 4 852.02 2.83 145 38 70 32 638.58 1.20 146 38 71 33 638.50 1.18 147 38 72 34 638.50 1.18 148 38 73 35 638.46 1.17 149 38 74 36 638.17 1.20 150 39 42 3 882.63 0.34 151 39 43 4 851.96 2.79 152 39 60 21 638.46 1.16 153 39 74 35 638.13 1.20 154 39 77 38 637.92 1.26 155 39 78 39 637.93 1.26 156 39 81 42 637.91 1.27 157 39 97 58 611.00 4.52 158 40 43 3 872.51 4.72 159 40 44 4 860.13 1.33 160 40 97 57 611.01 4.53 161 40 98 58 610.98 4.53 162 40 101 61 609.96 4.26 163 41 44 3 875.59 3.88 164 41 45 4 843.63 9.34 165 41 131 90 488.18 0.80 166 41 132 91 488.17 0.80 167 41 145 104 282.67 24.85 168 41 149 108 285.94 23.11 169 41 242 201 190.12 1.26 170 42 46 4 864.31 0.99 171 42 60 18 638.58 1.15 172 42 70 28 638.61 1.19 173 42 71 29 638.59 1.19 174 42 74 32 638.47 1.19 175 42 131 89 488.18 0.80 176 43 46 3 919.56 1.28 177 43 55 12 639.09 1.01 178 43 56 13 639.00 0.99 179 43 60 17 638.57 1.15 180 43 98 55 611.25 4.62 181 44 47 3 912.34 1.35 182 44 55 11 639.09 1.01 183 44 56 12 638.94 1.03 184 44 98 54 611.56 4.48 185 44 242 198 190.18 1.26 186 44 243 199 190.12 1.26 187 44 245 201 189.90 1.19 188 45 131 86 488.20 0.78 189 45 149 104 285.43 22.90 190 45 242 197 190.59 1.04 191 46 54 8 640.43 0.50 192 46 55 9 640.40 0.48 193 46 126 80 550.07 0.30 194 46 130 84 488.22 0.79 195 46 131 85 488.20 0.79 196 47 52 5 731.81 26.35 197 47 53 6 642.69 0.26 198 47 54 7 640.70 0.43 199 47 55 8 640.41 0.49 200 47 148 101 287.67 22.87 201 47 149 102 286.73 22.97 202 47 245 198 190.05 1.25 203 47 246 199 189.90 1.19 204 48 51 3 900.01 1.17 205 48 52 4 735.99 24.26 206 48 53 5 660.65 9.20 207 48 130 82 536.48 5.61 208 48 148 100 287.67 22.87 209 49 52 3 855.69 2.58 210 49 146 97 288.06 22.82 211 49 147 98 288.10 22.83 212 49 148 99 288.00 22.79 213 49 149 100 287.95 22.80 214 49 150 101 287.16 22.52 215 53 130 77 548.86 0.28 216 54 57 3 954.84 1.27 217 54 126 72 548.89 0.27 218 54 130 76 536.27 5.50 219 56 94 38 611.80 4.46 220 56 98 42 611.57 4.48 221 56 119 63 581.79 9.05 222 56 243 187 190.12 1.25 223 56 245 189 189.91 1.20 224 57 94 37 611.81 4.46 225 57 119 62 581.76 9.07 226 57 120 63 581.69 9.10 227 57 121 64 579.45 8.12 228 57 122 65 579.21 8.04 229 57 245 188 190.08 1.24 230 57 246 189 189.90 1.20 231 57 247 190 186.84 1.54 232 58 61 3 909.37 0.13 233 58 62 4 888.05 2.91 234 58 93 35 611.83 4.48 235 58 94 36 611.83 4.48 236 58 121 63 579.23 8.04 237 59 62 3 884.92 4.47 238 59 65 6 798.14 10.27 239 59 122 63 579.02 8.12 240 59 124 65 573.89 5.75 241 59 125 66 558.85 2.19 242 59 126 67 558.85 2.19 243 60 65 5 798.14 10.26 244 60 66 6 798.13 10.26 245 60 71 11 653.41 3.43 246 60 74 14 639.05 1.33 247 60 78 18 638.57 1.28 248 60 97 37 611.66 4.46 249 61 66 5 855.51 5.70 250 61 78 17 638.57 1.28 251 61 93 32 611.85 4.48 252 61 97 36 611.69 4.45 253 62 65 3 865.12 4.78 254 62 66 4 859.44 3.75 255 62 122 60 579.22 8.03 256 62 123 61 578.95 8.13 257 62 125 63 563.38 0.60 258 63 66 3 877.91 3.58 259 63 67 4 859.44 3.75 260 64 67 3 876.58 3.11 261 64 68 4 866.10 0.42 262 64 125 61 565.44 1.51 263 65 68 3 880.87 5.26 264 65 71 6 743.26 13.44 265 65 125 60 559.74 2.33 266 65 126 61 559.16 2.05 267 66 71 5 704.42 10.08 268 66 74 8 640.00 1.16 269 66 75 9 639.65 1.01 270 66 78 12 639.58 0.98 271 68 71 3 875.32 3.95 272 68 72 4 859.15 4.13 273 68 126 58 559.52 2.25 274 68 127 59 558.49 2.29 275 68 128 60 544.75 1.69 276 69 72 3 885.59 1.19 277 69 128 59 547.66 0.93 278 70 131 61 488.38 0.72 279 71 74 3 878.56 3.57 280 71 131 60 501.40 5.77 281 72 172 100 260.77 21.81 282 73 76 3 893.89 0.79 283 74 77 3 868.97 0.99 284 74 78 4 868.97 0.99 285 75 78 3 876.07 0.56 286 75 79 4 866.94 0.03 287 76 79 3 872.91 1.02 288 76 80 4 875.96 2.62 289 77 80 3 884.23 1.43 290 77 81 4 878.89 1.27 291 78 81 3 885.79 0.65 292 78 82 4 880.06 0.69 293 78 97 19 611.25 4.62 294 79 82 3 892.57 4.83 295 79 83 4 898.95 1.64 296 80 83 3 943.72 0.96 297 81 84 3 892.53 3.83 298 81 85 4 864.55 3.13 299 81 96 15 611.65 4.47 300 81 97 16 611.44 4.58 301 81 100 19 609.98 4.22 302 82 85 3 888.90 2.01 303 82 86 4 861.25 1.46 304 82 93 11 647.14 1.79 305 83 86 3 878.15 1.59 306 83 87 4 874.55 0.21 307 84 87 3 881.49 0.07 308 84 88 4 877.53 2.04 309 84 96 12 645.61 2.50 310 85 88 3 879.08 1.28 311 85 89 4 877.53 2.05 312 85 91 6 840.23 3.06 313 85 92 7 839.95 3.20 314 85 93 8 652.76 0.93 315 85 96 11 620.49 8.83 316 86 89 3 890.77 1.73 317 88 91 3 875.07 1.00 318 88 96 8 710.93 8.18 319 91 96 5 838.94 2.26 320 92 95 3 884.70 2.63 321 92 96 4 861.25 7.43 322 92 119 27 581.66 9.17 323 92 121 29 581.12 9.21 324 93 96 3 877.72 0.80 325 93 97 4 854.01 9.77 326 93 121 28 580.51 8.90 327 94 97 3 888.88 0.39 328 94 98 4 875.99 0.06 329 94 119 25 581.65 9.13 330 94 120 26 581.61 9.14 331 94 121 27 579.37 8.16 332 95 98 3 874.12 0.99 333 95 99 4 865.20 5.45 334 95 115 20 582.28 9.08 335 95 117 22 581.88 9.12 336 95 118 23 581.89 9.12 337 95 119 24 581.62 9.14 338 96 99 3 870.06 3.39 339 96 100 4 865.19 5.46 340 97 100 3 877.60 4.11 341 97 101 4 866.77 4.66 342 98 101 3 880.84 2.49 343 98 102 4 874.63 0.61 344 98 115 17 582.27 9.05 345 98 119 21 581.79 9.06 346 98 243 145 190.00 1.23 347 99 102 3 875.78 0.04 348 99 103 4 865.02 4.19 349 99 115 16 582.23 9.08 350 100 103 3 877.33 0.74 351 100 104 4 859.49 2.61 352 101 104 3 877.88 0.46 353 101 105 4 877.89 0.47 354 101 239 138 196.30 1.46 355 102 105 3 887.78 4.48 356 102 106 4 871.41 3.69 357 102 113 11 582.35 9.04 358 102 115 13 582.27 9.05 359 102 239 137 196.33 1.47 360 103 106 3 880.72 8.01 361 103 107 4 864.20 0.09 362 104 107 3 893.72 1.51 363 104 108 4 864.20 0.09 364 105 108 3 885.40 2.66 365 105 109 4 864.19 0.10 366 105 110 5 708.84 17.12 367 105 111 6 724.20 14.52 368 105 113 8 619.18 9.34 369 105 186 81 260.19 21.54 370 105 235 130 204.15 1.49 371 105 239 134 196.33 1.47 372 106 109 3 886.88 5.26 373 106 112 6 631.07 15.27 374 108 165 57 260.99 21.73 375 108 184 76 260.61 21.55 376 108 185 77 260.50 21.57 377 108 186 78 260.31 21.48 378 109 186 77 260.33 21.48 379 109 232 123 214.90 1.05 380 110 186 76 260.39 21.45 381 110 232 122 214.61 1.12 382 111 186 75 260.61 21.46 383 111 232 121 214.19 0.94 384 111 235 124 204.66 1.73 385 111 236 125 202.43 0.83 386 113 235 122 204.31 1.41 387 113 236 123 200.33 1.18 388 113 239 126 196.34 1.47 389 113 240 127 196.32 1.47 390 114 240 126 196.33 1.47 391 115 119 4 1078.05 1.32 392 116 240 124 196.34 1.47 393 116 244 128 190.12 1.26 394 116 247 131 189.38 1.26 395 117 247 130 189.87 1.20 396 118 247 129 189.87 1.20 397 119 243 124 192.62 1.75 398 119 244 125 190.30 1.20 399 119 247 128 184.59 0.77 400 126 130 4 897.57 0.30 401 127 130 3 961.78 1.05 402 130 144 14 288.59 22.57 403 132 140 8 288.93 22.49 404 132 141 9 288.89 22.49 405 132 144 12 288.89 22.49 406 132 145 13 288.87 22.50 407 133 136 3 877.51 0.61 408 133 140 7 295.64 25.72 409 134 175 41 274.83 15.33 410 134 205 71 257.31 6.66 411 134 206 72 257.38 6.62 412 134 207 73 257.26 6.64 413 135 175 40 274.88 15.35 414 135 206 71 257.41 6.62 415 136 140 4 844.14 7.75 416 136 207 71 257.46 6.62 417 137 140 3 883.00 2.17 418 137 141 4 846.38 8.88 419 137 210 73 257.50 6.50 420 138 141 3 859.84 4.75 421 138 142 4 852.82 3.43 422 138 210 72 257.52 6.50 423 138 214 76 255.78 5.63 424 138 217 79 239.18 2.67 425 139 142 3 871.64 3.48 426 139 143 4 849.05 4.95 427 140 143 3 847.95 4.41 428 140 144 4 846.06 3.46 429 141 144 3 874.31 6.13 430 141 145 4 840.45 6.26 431 141 156 15 292.94 21.48 432 141 207 66 260.26 5.14 433 141 210 69 260.25 5.13 434 141 211 70 260.12 5.07 435 141 217 76 241.79 4.22 436 142 146 4 729.61 4.00 437 142 147 5 729.61 4.00 438 142 153 11 293.31 21.52 439 142 156 14 293.25 21.53 440 142 214 72 258.66 4.19 441 142 216 74 242.08 4.11 442 142 217 75 241.81 4.24 443 143 147 4 698.73 11.44 444 143 148 5 698.73 11.44 445 144 148 4 312.88 31.04 446 144 149 5 312.87 31.04 447 145 149 4 465.13 107.19 448 145 150 5 309.10 29.21 449 145 151 6 308.43 29.02 450 145 156 11 293.31 21.53 451 145 159 14 305.82 27.79 452 146 150 4 309.00 29.19 453 146 151 5 307.22 28.40 454 149 242 93 196.25 1.49 455 149 245 96 190.09 1.24 456 151 155 4 847.52 0.08 457 151 159 8 797.26 1.38 458 151 241 90 198.19 0.82 459 151 242 91 197.25 1.10 460 151 245 94 190.59 1.02 461 152 155 3 862.04 7.16 462 152 156 4 862.05 7.16 463 152 241 89 198.59 0.73 464 153 156 3 872.89 1.74 465 153 157 4 872.60 1.88 466 153 216 63 265.08 16.11 467 153 217 64 265.08 16.10 468 153 220 67 263.62 16.83 469 154 157 3 879.54 1.57 470 154 158 4 866.36 1.24 471 154 220 66 262.56 17.36 472 154 224 70 261.70 17.78 473 154 234 80 213.81 0.64 474 155 158 3 870.47 0.52 475 155 159 4 866.29 1.28 476 155 238 83 199.38 0.68 477 155 241 86 197.93 0.77 478 156 159 3 873.26 2.21 479 156 160 4 866.35 1.25 480 156 211 55 283.41 7.34 481 156 217 61 265.46 16.23 482 157 160 3 871.28 1.22 483 157 161 4 866.31 1.23 484 157 191 34 300.32 1.20 485 157 211 54 283.38 7.33 486 157 217 60 265.33 16.30 487 157 220 63 262.86 17.50 488 157 221 64 262.51 17.68 489 157 224 67 261.27 18.30 490 158 161 3 879.86 3.07 491 158 224 66 261.00 18.46 492 158 231 73 218.43 2.73 493 158 234 76 213.58 0.70 494 158 235 77 204.47 1.61 495 158 238 80 198.92 0.91 496 159 162 3 892.34 3.17 497 159 235 76 202.86 0.81 498 159 238 79 198.11 0.85 499 159 239 80 197.20 1.04 500 159 242 83 191.97 0.38 501 160 163 3 898.47 0.10 502 160 190 30 335.75 16.47 503 160 211 51 283.39 7.36 504 161 164 3 885.28 1.92 505 161 190 29 335.72 16.46 506 161 191 30 335.75 16.48 507 161 231 70 218.56 2.79 508 162 165 3 898.30 4.12 509 162 186 24 343.75 19.71 510 162 235 73 202.81 0.83 511 163 179 16 336.75 16.00 512 163 190 27 341.58 19.39 513 163 205 42 288.75 4.98 514 163 208 45 284.57 7.04 515 164 180 16 343.12 19.22 516 164 185 21 344.12 19.72 517 164 186 22 344.09 19.73 518 164 187 23 343.67 19.82 519 164 190 26 341.37 19.50 520 165 183 18 344.10 19.71 521 165 184 19 344.12 19.72 522 165 185 20 344.12 19.73 523 165 186 21 344.07 19.75 524 166 178 12 345.13 19.32 525 166 179 13 345.12 19.32 526 166 183 17 344.16 19.75 527 167 177 10 345.24 19.38 528 167 178 11 345.13 19.32 529 167 179 12 345.13 19.32 530 168 173 5 433.31 9.19 531 168 175 7 345.77 19.41 532 168 176 8 345.31 19.37 533 168 177 9 345.28 19.37 534 168 179 11 345.28 19.36 535 168 203 35 330.78 25.62 536 169 172 3 862.53 0.07 537 169 173 4 789.43 15.56 538 169 174 5 418.25 16.72 539 169 175 6 346.06 19.35 540 169 176 7 345.38 19.34 541 170 174 4 418.25 16.72 542 170 175 5 346.09 19.36 543 170 176 6 346.06 19.35 544 175 203 28 330.81 25.62 545 175 205 30 330.67 25.68 546 175 206 31 330.74 25.64 547 176 203 27 330.80 25.62 548 177 203 26 330.81 25.61 549 178 183 5 345.41 19.24 550 178 201 23 330.93 25.62 551 178 202 24 330.86 25.62 552 178 203 25 330.78 25.62 553 179 190 11 343.52 20.04 554 179 193 14 340.50 21.50 555 179 202 23 330.90 25.61 556 179 203 24 330.77 25.62 557 179 205 26 330.75 25.61 558 179 208 29 325.05 27.16 559 180 183 3 874.07 5.72 560 180 189 9 343.86 19.89 561 180 190 10 343.53 20.04 562 180 193 13 341.67 20.95 563 180 202 22 330.94 25.62 564 181 199 18 331.02 25.64 565 181 202 21 330.94 25.63 566 182 185 3 865.22 1.88 567 186 231 45 247.80 17.43 568 186 232 46 243.44 15.30 569 186 235 49 232.15 15.49 570 187 228 41 254.84 20.96 571 187 231 44 247.79 17.43 572 187 232 45 243.44 15.30 573 188 191 3 879.03 1.59 574 188 192 4 873.78 2.94 575 188 224 36 261.14 18.54 576 188 225 37 261.10 18.53 577 188 228 40 254.80 20.93 578 188 231 43 243.55 15.30 579 189 192 3 873.78 2.94 580 189 193 4 871.85 3.92 581 190 193 3 877.48 1.19 582 190 194 4 865.88 0.90 583 190 208 18 324.94 27.12 584 190 211 21 323.45 27.23 585 191 194 3 868.78 0.36 586 191 195 4 866.81 1.35 587 191 221 30 262.23 18.07 588 191 224 33 261.18 18.55 589 191 225 34 261.10 18.53 590 191 231 40 243.41 15.22 591 192 195 3 881.50 1.90 592 192 196 4 865.99 0.94 593 192 225 33 261.12 18.53 594 193 196 3 871.71 3.00 595 193 197 4 861.49 1.30 596 193 202 9 330.97 25.64 597 193 204 11 330.85 25.64 598 194 197 3 888.78 0.93 599 194 204 10 330.84 25.64 600 194 208 14 324.96 27.12 601 194 209 15 324.93 27.14 602 194 211 17 323.66 26.60 603 194 212 18 322.82 26.93 604 194 221 27 263.36 17.75 605 195 212 17 322.82 26.93 606 195 218 23 265.88 16.54 607 195 221 26 264.43 17.23 608 195 222 27 263.35 17.75 609 195 225 30 261.48 18.56 610 197 204 7 330.97 25.64 611 198 204 6 330.97 25.64 612 198 209 11 330.23 25.50 613 199 202 3 880.69 1.09 614 204 208 4 852.27 4.10 615 204 209 5 850.13 3.04 616 205 208 3 863.18 1.34 617 205 209 4 852.34 4.11 618 206 209 3 867.43 0.79 619 206 210 4 858.37 2.95 620 207 210 3 865.30 0.80 621 207 211 4 866.33 1.02 622 208 211 3 886.03 0.91 623 209 212 3 886.03 0.91 624 209 213 4 866.35 1.02 625 210 213 3 891.96 2.06 626 210 214 4 864.21 2.08 627 211 217 6 265.88 16.54 628 211 218 7 265.87 16.54 629 211 221 10 264.44 17.23 630 212 218 6 323.83 12.47 631 212 221 9 264.93 16.98 632 214 217 3 866.33 1.04 633 214 218 4 860.74 3.33 634 215 219 4 880.77 2.30 635 216 219 3 915.70 1.98 636 216 220 4 874.87 5.26 637 217 220 3 883.03 3.48 638 217 221 4 872.52 6.43 639 218 221 3 872.05 3.85 640 218 222 4 869.70 5.03 641 219 222 3 869.26 5.25 642 219 223 4 862.59 1.91 643 220 223 3 862.67 1.95 644 220 224 4 862.59 1.91 645 221 224 3 868.07 0.76 646 221 225 4 867.62 0.97 647 222 225 3 872.05 2.80 648 222 226 4 855.37 5.53 649 223 226 3 868.01 0.79 650 223 227 4 853.86 4.76 651 223 230 7 737.25 7.45 652 223 234 11 716.47 17.82 653 224 227 3 864.47 1.68 654 224 230 6 737.25 7.45 655 224 231 7 525.77 66.50 656 224 234 10 711.91 20.11 657 227 230 3 866.09 0.01 658 227 231 4 835.59 15.26 659 228 231 3 862.20 1.96 660 228 232 4 855.39 5.36 661 229 232 3 866.50 0.19 662 229 233 4 863.81 1.16 663 230 233 3 872.64 4.00 664 230 234 4 835.26 4.05 665 231 234 3 876.15 5.19 666 231 235 4 842.23 0.62 667 232 235 3 865.72 1.63 668 232 236 4 842.24 0.63 669 233 236 3 875.92 1.44 670 233 237 4 861.78 3.59 671 234 237 3 884.78 0.86 672 234 238 4 861.77 3.58 673 235 238 3 874.70 6.43 674 235 239 4 856.70 1.03 675 236 239 3 867.52 3.24 676 236 240 4 862.52 3.94 677 237 240 3 866.30 2.81 678 237 241 4 862.52 3.94 679 238 241 3 867.66 6.50 680 238 242 4 847.88 3.39 681 239 242 3 858.24 6.72 682 239 243 4 840.90 0.10 683 240 243 3 882.94 1.68 684 240 244 4 866.22 2.70 685 241 244 3 870.45 4.82 686 241 245 4 855.37 8.12 687 242 245 3 879.26 3.69 688 244 247 3 856.40 2.33 689 246 250 4 736.59 11.55 690 247 250 3 912.45 1.16