BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 78 74 639.19 0.18 2 4 80 76 634.51 0.44 3 5 33 28 684.02 21.34 4 5 34 29 684.01 21.34 5 5 35 30 683.97 21.32 6 5 76 71 642.42 0.72 7 6 80 74 635.25 0.27 8 6 82 76 634.34 0.49 9 7 32 25 684.21 21.41 10 7 33 26 684.16 21.41 11 7 74 67 635.25 0.27 12 7 75 68 642.71 0.71 13 7 76 69 642.82 0.76 14 7 80 73 642.64 0.76 15 7 81 74 641.57 0.54 16 7 82 75 634.81 0.49 17 8 30 22 684.56 21.45 18 8 31 23 684.38 21.43 19 8 32 24 684.29 21.39 20 8 34 26 684.28 21.37 21 9 31 22 684.47 21.46 22 9 72 63 640.23 1.21 23 9 74 65 643.02 0.86 24 9 82 73 642.63 0.77 25 9 83 74 640.72 0.96 26 9 84 75 634.71 0.50 27 10 28 18 684.68 21.50 28 10 30 20 684.68 21.48 29 10 31 21 684.54 21.46 30 10 72 62 643.23 0.96 31 10 84 74 639.69 1.47 32 11 26 15 685.11 21.59 33 11 27 16 685.07 21.57 34 11 28 17 684.66 21.50 35 11 43 32 684.00 21.33 36 11 70 59 643.28 0.99 37 11 72 61 643.24 0.96 38 11 84 73 642.85 0.78 39 11 86 75 641.40 0.49 40 12 26 14 685.15 21.57 41 12 27 15 685.12 21.57 42 13 16 3 889.40 0.62 43 13 24 11 686.82 21.96 44 13 25 12 685.74 21.62 45 13 26 13 685.20 21.54 46 13 86 73 642.88 0.78 47 13 87 74 642.83 0.79 48 13 88 75 638.88 0.72 49 14 24 10 686.85 21.98 50 14 25 11 686.79 21.97 51 15 18 3 841.80 4.92 52 15 22 7 731.50 0.93 53 15 23 8 720.62 5.65 54 15 24 9 686.88 21.98 55 18 22 4 787.12 3.25 56 18 24 6 729.82 1.58 57 18 88 70 642.89 0.76 58 18 89 71 642.69 0.82 59 18 90 72 640.29 0.79 60 18 91 73 640.55 0.92 61 19 22 3 845.89 0.21 62 19 90 71 642.40 0.74 63 19 91 72 641.45 0.47 64 19 93 74 617.11 3.77 65 20 62 42 711.02 5.21 66 20 63 43 711.02 5.19 67 20 64 44 710.95 5.14 68 20 65 45 710.30 4.83 69 20 90 70 642.73 0.83 70 20 91 71 642.39 0.74 71 20 92 72 634.73 0.43 72 20 93 73 626.76 1.06 73 21 61 40 711.29 5.16 74 21 62 41 711.12 5.24 75 21 63 42 711.07 5.18 76 22 61 39 714.04 3.78 77 23 59 36 714.50 3.62 78 23 60 37 714.31 3.68 79 23 61 38 714.09 3.75 80 24 59 35 714.48 3.60 81 24 60 36 714.46 3.60 82 24 88 64 642.85 0.80 83 25 57 32 714.64 3.54 84 25 58 33 714.56 3.56 85 25 59 34 714.46 3.57 86 26 43 17 716.61 5.04 87 26 45 19 716.96 4.85 88 26 57 31 714.62 3.52 89 26 58 32 714.60 3.53 90 26 60 34 714.54 3.50 91 26 70 44 676.04 15.29 92 26 88 62 642.42 0.79 93 27 56 29 714.69 3.49 94 27 57 30 714.57 3.50 95 28 31 3 833.13 0.80 96 28 41 13 717.20 4.75 97 28 43 15 717.18 4.75 98 28 56 28 714.65 3.47 99 28 72 44 675.88 15.33 100 29 56 27 714.63 3.45 101 31 38 7 731.98 2.90 102 31 41 10 720.34 5.98 103 31 72 41 676.20 15.52 104 31 74 43 675.77 15.43 105 32 38 6 731.99 2.90 106 33 36 3 822.18 2.06 107 33 37 4 778.66 0.51 108 33 38 5 758.76 10.44 109 33 76 43 675.64 15.50 110 33 77 44 675.64 15.50 111 36 77 41 675.65 15.51 112 37 76 39 675.70 15.50 113 37 77 40 675.65 15.50 114 38 41 3 948.23 0.28 115 38 74 36 676.10 15.55 116 38 75 37 676.00 15.54 117 38 76 38 675.67 15.47 118 39 75 36 676.00 15.54 119 39 76 37 675.79 15.45 120 39 77 38 675.75 15.43 121 40 74 34 676.10 15.52 122 40 75 35 676.02 15.51 123 41 52 11 723.16 0.66 124 41 53 12 723.09 0.67 125 41 55 14 723.07 0.65 126 41 56 15 722.98 0.65 127 41 72 31 676.27 15.47 128 41 73 32 676.21 15.49 129 41 74 33 676.06 15.50 130 42 51 9 729.52 2.44 131 42 52 10 729.34 2.43 132 42 72 30 676.25 15.47 133 42 73 31 676.20 15.46 134 43 50 7 730.07 2.37 135 43 51 8 729.68 2.52 136 43 52 9 729.52 2.46 137 43 53 10 729.48 2.44 138 43 56 13 723.15 0.65 139 43 58 15 723.00 0.64 140 43 70 27 676.43 15.48 141 43 71 28 676.32 15.46 142 43 72 29 676.26 15.46 143 44 49 5 749.94 0.27 144 44 50 6 733.39 0.70 145 44 70 26 676.45 15.47 146 44 71 27 676.33 15.46 147 45 49 4 770.86 6.78 148 45 50 5 749.91 0.26 149 45 51 6 749.91 0.26 150 45 58 13 723.10 0.64 151 45 60 15 722.87 0.65 152 45 62 17 721.91 0.30 153 45 68 23 676.83 15.56 154 45 69 24 676.84 15.55 155 45 70 25 676.45 15.47 156 46 68 22 676.83 15.56 157 46 69 23 676.84 15.55 158 47 60 13 723.09 0.64 159 47 62 15 722.87 0.66 160 47 68 21 676.91 15.52 161 49 60 11 723.01 0.64 162 52 58 6 728.25 1.18 163 53 57 4 777.49 3.77 164 53 58 5 728.25 1.18 165 62 68 6 739.21 3.52 166 62 70 8 730.07 2.69 167 62 88 26 642.99 0.76 168 62 90 28 641.82 0.64 169 64 68 4 927.52 0.73 170 65 90 25 642.88 0.80 171 65 91 26 642.72 0.84 172 65 92 27 642.48 0.79 173 65 97 32 521.53 1.25 174 66 89 23 642.99 0.77 175 66 90 24 642.94 0.77 176 67 87 20 643.13 0.75 177 67 88 21 643.06 0.76 178 67 89 22 642.92 0.76 179 68 87 19 643.10 0.73 180 68 88 20 643.01 0.75 181 68 90 22 642.91 0.76 182 69 85 16 643.22 0.71 183 69 86 17 643.13 0.73 184 69 87 18 642.98 0.73 185 70 85 15 643.12 0.72 186 70 86 16 643.09 0.73 187 70 88 18 642.81 0.68 188 71 83 12 643.26 0.68 189 71 84 13 643.14 0.72 190 71 85 14 643.02 0.70 191 72 83 11 643.12 0.72 192 72 84 12 643.07 0.72 193 73 81 8 643.33 0.66 194 73 82 9 643.25 0.68 195 73 83 10 643.03 0.70 196 74 81 7 643.27 0.66 197 74 82 8 643.23 0.67 198 75 79 4 782.73 4.99 199 75 80 5 696.08 26.95 200 75 81 6 643.23 0.64 201 76 79 3 830.03 1.42 202 76 80 4 755.52 2.65 203 92 97 5 772.83 18.57 204 95 131 36 67.89 0.42 205 96 101 5 481.78 3.15 206 96 103 7 68.42 0.48 207 96 130 34 68.21 0.46 208 96 131 35 68.28 0.43 209 96 245 149 68.21 0.47 210 96 246 150 67.75 0.40 211 98 101 3 807.85 2.56 212 98 246 148 68.22 0.46 213 98 247 149 68.14 0.43 214 98 248 150 67.72 0.40 215 99 247 148 68.26 0.45 216 99 248 149 68.09 0.43 217 99 249 150 67.92 0.46 218 99 251 152 66.64 0.93 219 100 108 8 74.70 0.52 220 100 110 10 74.70 0.52 221 100 151 51 73.54 1.08 222 100 156 56 69.48 1.01 223 100 228 128 69.39 0.97 224 100 246 146 68.36 0.45 225 100 247 147 68.31 0.43 226 100 249 149 68.31 0.43 227 101 133 32 79.47 0.44 228 101 245 144 69.41 0.96 229 102 105 3 829.47 1.80 230 102 106 4 795.62 8.68 231 102 107 5 748.19 6.21 232 102 110 8 482.11 5.68 233 102 123 21 85.18 0.35 234 102 133 31 79.48 0.44 235 102 134 32 79.37 0.40 236 102 135 33 80.01 0.72 237 102 245 143 75.34 0.17 238 103 130 27 79.63 0.51 239 103 131 28 79.62 0.51 240 103 132 29 80.15 0.25 241 103 133 30 79.64 0.51 242 105 123 18 95.48 0.81 243 105 133 28 93.28 0.25 244 106 123 17 96.70 0.24 245 107 110 3 824.93 5.31 246 107 121 14 106.32 1.24 247 107 122 15 105.81 1.46 248 107 123 16 103.20 1.02 249 107 135 28 97.75 0.09 250 108 151 43 106.77 1.02 251 109 151 42 106.80 1.00 252 110 135 25 106.81 1.02 253 110 149 39 110.67 0.68 254 110 150 40 109.59 0.39 255 110 151 41 106.80 1.00 256 110 228 118 109.58 0.39 257 110 247 137 106.77 1.01 258 111 121 10 117.98 0.10 259 111 149 38 116.92 0.22 260 111 150 39 109.94 0.23 261 112 119 7 118.16 0.04 262 112 120 8 118.16 0.04 263 112 121 9 118.14 0.04 264 112 137 25 118.14 0.04 265 112 148 36 117.93 0.12 266 112 149 37 117.28 0.06 267 113 147 34 118.20 0.03 268 113 148 35 118.16 0.04 269 113 150 37 118.15 0.04 270 114 117 3 819.93 1.46 271 114 146 32 119.81 0.24 272 114 147 33 118.20 0.03 273 114 148 34 118.20 0.03 274 115 145 30 157.09 1.07 275 115 146 31 138.80 0.79 276 116 141 25 169.44 0.61 277 116 142 26 169.16 0.48 278 116 143 27 168.99 0.43 279 116 144 28 168.71 0.43 280 116 145 29 163.72 1.35 281 117 139 22 169.69 0.65 282 117 140 23 169.60 0.63 283 117 141 24 169.44 0.61 284 117 146 29 167.27 0.05 285 117 147 30 162.72 1.84 286 117 234 117 168.50 0.53 287 118 139 21 169.72 0.67 288 118 140 22 169.70 0.65 289 119 137 18 169.74 0.66 290 119 138 19 169.72 0.67 291 119 139 20 169.72 0.67 292 120 137 17 169.77 0.67 293 120 138 18 169.72 0.66 294 121 135 14 170.09 0.52 295 121 136 15 169.83 0.69 296 121 137 16 169.78 0.66 297 121 149 28 169.46 0.65 298 122 135 13 170.14 0.54 299 122 136 14 170.16 0.55 300 123 133 10 171.49 0.06 301 123 134 11 171.44 0.06 302 123 135 12 170.16 0.54 303 124 127 3 875.25 0.09 304 124 133 9 171.50 0.07 305 124 134 10 171.46 0.05 306 124 135 11 171.47 0.06 307 124 136 12 171.50 0.06 308 124 243 119 171.74 0.01 309 125 132 7 187.44 0.33 310 125 133 8 171.74 0.05 311 126 136 10 188.16 0.04 312 126 243 117 188.17 0.04 313 127 168 41 188.22 0.04 314 127 209 82 188.24 0.04 315 127 243 116 188.17 0.04 316 128 131 3 870.77 3.32 317 128 132 4 782.85 7.08 318 128 133 5 467.76 0.36 319 128 134 6 188.16 0.04 320 128 209 81 188.28 0.05 321 128 245 117 188.12 0.04 322 129 166 37 188.16 0.04 323 129 209 80 188.29 0.05 324 129 211 82 188.23 0.06 325 129 245 116 188.16 0.04 326 129 246 117 188.12 0.05 327 130 245 115 188.25 0.06 328 130 246 116 188.13 0.05 329 133 245 112 188.44 0.05 330 134 244 110 188.53 0.07 331 134 245 111 188.44 0.05 332 135 149 14 198.24 2.46 333 135 243 108 189.10 0.05 334 135 244 109 188.55 0.07 335 136 241 105 198.68 2.45 336 136 242 106 198.49 2.46 337 136 243 107 193.66 2.27 338 137 147 10 200.62 1.57 339 137 148 11 198.75 2.48 340 137 149 12 198.59 2.48 341 137 230 93 198.58 2.49 342 137 232 95 198.94 2.40 343 137 241 104 198.69 2.45 344 137 242 105 198.50 2.46 345 138 239 101 206.43 1.42 346 138 240 102 200.69 1.55 347 138 241 103 198.69 2.45 348 139 147 8 200.69 1.56 349 139 239 100 212.53 1.61 350 139 240 101 201.31 1.59 351 140 238 98 235.11 0.17 352 140 239 99 213.86 1.36 353 141 174 33 247.08 0.84 354 141 234 93 222.86 0.94 355 141 236 95 264.67 0.86 356 141 237 96 262.35 0.13 357 141 238 97 251.56 0.48 358 141 240 99 236.78 0.75 359 142 237 95 264.19 0.63 360 143 234 91 266.22 0.49 361 143 236 93 265.76 0.46 362 143 237 94 265.69 0.48 363 144 234 90 266.23 0.49 364 144 235 91 266.16 0.58 365 145 233 88 266.43 0.53 366 145 234 89 266.33 0.54 367 146 231 85 266.60 0.52 368 146 232 86 266.44 0.53 369 146 233 87 266.44 0.54 370 147 231 84 266.64 0.51 371 147 232 85 266.50 0.56 372 148 181 33 267.20 0.64 373 148 230 82 266.77 0.46 374 148 231 83 266.66 0.52 375 149 229 80 267.13 0.60 376 149 230 81 267.00 0.56 377 149 244 95 266.30 0.59 378 150 181 31 267.66 0.60 379 150 227 77 276.28 3.33 380 150 228 78 267.66 0.60 381 150 229 79 267.28 0.64 382 151 156 5 824.31 1.99 383 151 227 76 276.32 3.31 384 151 228 77 267.83 0.53 385 152 226 74 305.05 1.36 386 152 227 75 280.52 1.63 387 153 156 3 869.08 1.31 388 153 225 72 316.15 1.05 389 153 226 73 298.00 1.20 390 153 227 74 285.08 0.61 391 154 225 71 317.33 0.74 392 154 226 72 317.05 0.61 393 155 222 67 343.58 3.12 394 155 223 68 333.50 1.92 395 155 224 69 327.74 0.85 396 155 225 70 323.38 0.47 397 155 226 71 322.70 0.35 398 156 226 70 326.69 1.07 399 156 228 72 322.68 0.88 400 156 249 93 265.75 0.57 401 157 161 4 795.37 2.19 402 157 163 6 738.18 1.83 403 157 182 25 328.96 0.18 404 157 216 59 345.67 1.01 405 157 226 69 329.82 0.25 406 157 249 92 265.71 0.56 407 158 161 3 836.63 1.60 408 158 216 58 374.80 1.80 409 159 216 57 374.75 1.76 410 159 217 58 374.78 1.68 411 159 218 59 374.64 1.62 412 159 220 61 374.50 1.55 413 159 222 63 374.35 1.45 414 160 215 55 375.15 1.74 415 160 216 56 374.94 1.73 416 160 217 57 374.87 1.73 417 160 218 58 374.64 1.62 418 161 215 54 375.31 1.77 419 161 216 55 375.05 1.69 420 161 249 88 265.70 0.56 421 162 213 51 376.66 2.04 422 162 214 52 376.19 2.21 423 162 215 53 375.94 2.09 424 162 249 87 265.48 0.48 425 163 182 19 375.24 1.15 426 163 213 50 376.73 2.02 427 163 214 51 376.61 2.02 428 163 215 52 376.68 1.97 429 163 216 53 376.79 1.92 430 163 228 65 374.81 1.23 431 163 248 85 265.79 0.60 432 163 249 86 265.17 0.34 433 164 211 47 377.72 1.82 434 164 212 48 377.42 1.78 435 164 213 49 376.73 2.02 436 164 246 82 267.12 0.98 437 164 247 83 265.90 0.66 438 164 248 84 265.78 0.60 439 165 180 15 374.80 1.22 440 165 210 45 381.18 0.35 441 165 211 46 378.02 1.67 442 165 212 47 377.49 1.81 443 165 214 49 377.42 1.78 444 165 228 63 375.75 1.11 445 165 230 65 374.82 1.21 446 165 247 82 265.80 0.60 447 166 209 43 403.45 0.42 448 166 210 44 381.18 0.35 449 166 211 45 378.05 1.69 450 166 244 78 268.80 0.65 451 166 245 79 267.51 1.01 452 166 246 80 267.19 1.02 453 166 248 82 267.00 1.10 454 167 178 11 391.22 5.58 455 167 180 13 375.85 1.00 456 167 209 42 403.48 0.42 457 167 210 43 384.93 1.53 458 167 212 45 380.77 0.55 459 167 230 63 375.78 1.02 460 167 242 75 275.40 2.98 461 167 243 76 275.24 2.93 462 167 244 77 270.37 1.45 463 168 207 39 418.46 2.66 464 168 208 40 416.92 2.44 465 168 209 41 403.49 0.42 466 168 242 74 275.40 2.97 467 168 243 75 275.31 2.94 468 169 175 6 771.75 2.64 469 169 178 9 417.19 2.15 470 169 201 32 418.63 2.54 471 169 204 35 418.45 2.66 472 169 206 37 418.42 2.65 473 169 207 38 418.47 2.66 474 169 208 39 418.43 2.64 475 169 210 41 417.58 2.50 476 170 173 3 868.18 0.88 477 170 201 31 427.36 0.24 478 170 206 36 418.54 2.64 479 170 207 37 418.47 2.66 480 170 240 70 293.84 2.93 481 170 241 71 285.28 1.31 482 170 242 72 279.97 0.83 483 171 201 30 439.78 3.96 484 171 206 35 419.53 3.12 485 171 207 36 419.54 3.12 486 172 201 29 444.80 4.08 487 173 201 28 444.89 4.03 488 173 240 67 293.97 2.97 489 174 234 60 375.75 1.11 490 174 235 61 375.73 1.11 491 174 236 62 375.14 1.33 492 174 237 63 374.75 1.17 493 174 238 64 352.77 0.04 494 174 240 66 311.73 1.81 495 175 201 26 444.99 4.08 496 175 232 57 376.00 1.04 497 175 233 58 375.93 1.04 498 175 234 59 375.83 1.06 499 175 235 60 375.83 1.06 500 175 240 65 303.16 6.01 501 176 200 24 445.12 4.09 502 176 201 25 445.00 4.08 503 176 233 57 375.93 1.04 504 176 235 59 375.72 1.13 505 177 198 21 445.79 3.94 506 177 199 22 445.20 4.05 507 177 200 23 445.12 4.08 508 177 201 24 445.10 4.07 509 177 232 55 376.00 1.07 510 177 233 56 375.92 1.06 511 178 198 20 445.81 3.94 512 178 199 21 445.57 4.03 513 178 204 26 445.13 4.08 514 178 230 52 377.28 1.21 515 178 231 53 376.76 1.43 516 178 232 54 375.94 1.08 517 178 242 64 276.15 2.53 518 179 194 15 452.75 0.97 519 179 196 17 447.07 3.44 520 179 197 18 445.92 4.00 521 179 198 19 445.80 3.94 522 179 230 51 377.29 1.21 523 179 231 52 377.13 1.28 524 180 196 16 447.13 3.46 525 180 197 17 447.11 3.45 526 180 212 32 443.03 4.34 527 180 214 34 442.94 4.37 528 180 229 49 377.69 1.29 529 180 230 50 377.30 1.21 530 181 194 13 452.89 1.04 531 181 195 14 451.87 1.33 532 181 196 15 447.13 3.45 533 181 214 33 443.00 4.34 534 181 227 46 379.69 0.74 535 181 228 47 378.47 0.99 536 181 229 48 377.75 1.31 537 181 230 49 377.58 1.34 538 181 231 50 377.54 1.32 539 182 194 12 452.92 1.04 540 182 195 13 452.05 1.26 541 182 214 32 443.01 4.35 542 182 216 34 441.40 3.88 543 182 226 44 379.93 0.64 544 182 227 45 379.65 0.74 545 182 228 46 378.55 1.02 546 183 189 6 786.00 3.39 547 183 192 9 464.28 6.50 548 183 193 10 453.22 1.19 549 183 194 11 452.93 1.04 550 183 226 43 385.22 2.00 551 183 227 44 379.72 0.72 552 184 192 8 472.95 2.19 553 184 193 9 453.34 1.16 554 184 195 11 453.25 1.21 555 184 216 32 442.98 4.36 556 184 220 36 432.69 2.10 557 184 224 40 398.32 1.46 558 184 225 41 397.48 1.23 559 184 226 42 385.31 1.97 560 185 189 4 769.24 5.00 561 185 191 6 499.62 2.32 562 185 192 7 472.95 2.19 563 185 224 39 398.37 1.47 564 185 225 40 398.26 1.46 565 185 226 41 397.46 1.63 566 186 189 3 819.93 2.58 567 186 190 4 806.60 4.08 568 186 191 5 745.27 4.07 569 186 224 38 398.94 1.20 570 186 225 39 398.92 1.20 571 187 225 38 398.94 1.20 572 189 192 3 854.74 0.67 573 189 227 38 398.39 1.47 574 191 224 33 420.95 0.14 575 193 220 27 434.61 1.45 576 193 224 31 403.11 3.31 577 195 214 19 443.03 4.36 578 195 216 21 441.79 4.06 579 195 217 22 440.88 3.62 580 196 214 18 442.98 4.36 581 197 212 15 443.03 4.34 582 197 213 16 443.00 4.35 583 197 214 17 442.25 4.06 584 198 203 5 826.93 5.42 585 199 203 4 816.27 0.17 586 199 204 5 797.45 0.61 587 199 212 13 443.01 4.35 588 200 203 3 833.67 2.06 589 200 204 4 816.28 0.17 590 201 204 3 841.11 4.17 591 201 206 5 802.83 2.08 592 204 210 6 616.31 63.82 593 205 208 3 823.97 2.23 594 205 209 4 800.88 0.97 595 205 210 5 642.24 53.37 596 209 243 34 275.67 2.84 597 209 244 35 275.41 2.97 598 209 245 36 275.37 2.96 599 216 220 4 838.78 0.34 600 216 226 10 386.62 1.31 601 217 220 3 848.40 4.17 602 221 224 3 828.16 1.70 603 228 247 19 265.87 0.57 604 228 249 21 265.72 0.56 605 230 244 14 267.19 1.02 606 232 240 8 370.12 43.52 607 232 242 10 276.19 2.55 608 234 240 6 758.26 1.43 609 244 247 3 966.40 0.56