BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 202 198 74.36 2.44 2 4 225 221 82.25 0.16 3 5 184 179 121.02 7.07 4 5 186 181 121.83 6.69 5 5 203 198 110.81 7.70 6 5 204 199 92.09 1.66 7 5 225 220 92.10 1.66 8 6 9 3 905.22 1.99 9 6 186 180 129.87 6.99 10 6 201 195 125.62 9.16 11 6 202 196 116.15 7.44 12 6 222 216 98.74 0.66 13 6 223 217 110.33 1.02 14 6 225 219 92.12 1.65 15 7 191 184 129.86 6.99 16 7 195 188 134.53 6.73 17 7 201 194 131.14 6.40 18 7 203 196 121.84 6.72 19 8 194 186 134.50 6.71 20 8 195 187 134.50 6.71 21 8 199 191 134.63 6.77 22 8 200 192 134.50 6.74 23 8 201 193 134.12 6.81 24 8 222 214 112.53 0.08 25 9 200 191 134.60 6.75 26 9 222 213 112.73 0.14 27 10 200 190 139.33 4.42 28 10 221 211 113.59 0.09 29 10 222 212 112.91 0.17 30 10 223 213 112.89 0.18 31 11 200 189 144.88 2.44 32 11 220 209 117.02 1.23 33 11 221 210 113.59 0.09 34 11 223 212 113.07 0.16 35 11 239 228 36.47 0.45 36 11 244 233 31.22 0.01 37 11 246 235 29.70 0.36 38 12 157 145 187.31 2.28 39 12 177 165 176.12 3.24 40 12 219 207 117.26 1.31 41 12 220 208 117.09 1.24 42 13 157 144 201.08 1.09 43 13 177 164 197.06 2.02 44 13 217 204 119.21 1.02 45 13 218 205 119.09 0.99 46 13 219 206 117.28 1.32 47 13 239 226 41.21 0.84 48 13 240 227 34.48 1.07 49 13 244 231 32.70 0.20 50 14 157 143 202.49 0.39 51 14 177 163 200.26 0.66 52 14 217 203 119.21 1.02 53 14 218 204 119.14 1.01 54 15 156 141 205.11 0.79 55 15 157 142 205.11 0.80 56 15 215 200 122.80 0.42 57 15 216 201 122.58 0.36 58 15 217 202 119.22 1.02 59 16 157 141 205.12 0.79 60 16 159 143 205.49 0.86 61 16 212 196 132.88 5.33 62 16 214 198 124.74 1.39 63 16 215 199 122.80 0.42 64 16 216 200 122.75 0.43 65 16 218 202 119.22 1.02 66 17 152 135 206.39 0.95 67 17 156 139 205.69 0.83 68 17 157 140 205.75 0.86 69 17 158 141 205.63 0.80 70 17 159 142 205.64 0.80 71 17 214 197 124.77 1.41 72 18 159 141 205.83 0.89 73 18 209 191 163.57 0.20 74 18 212 194 136.60 5.51 75 18 213 195 132.72 5.24 76 18 214 196 123.91 0.97 77 19 149 130 206.55 0.89 78 19 158 139 205.94 0.91 79 19 159 140 205.91 0.92 80 19 160 141 205.86 0.89 81 19 163 144 205.96 0.95 82 19 167 148 205.99 0.94 83 21 139 118 221.91 0.77 84 21 147 126 207.39 0.78 85 22 139 117 221.97 0.78 86 22 144 122 221.97 0.77 87 22 145 123 221.85 0.75 88 23 144 121 222.90 0.36 89 24 135 111 221.94 0.76 90 24 138 114 222.38 0.95 91 24 139 115 222.05 0.79 92 24 142 118 223.42 0.56 93 24 144 120 222.90 0.36 94 25 142 117 223.46 0.57 95 26 101 75 223.47 0.59 96 26 102 76 223.47 0.59 97 26 105 79 223.43 0.61 98 27 99 72 223.74 0.47 99 27 101 74 223.62 0.52 100 27 102 75 223.47 0.59 101 30 96 66 420.97 27.61 102 30 97 67 420.48 27.72 103 30 98 68 370.49 3.67 104 31 35 4 826.29 2.68 105 31 36 5 736.17 1.44 106 31 39 8 680.15 2.83 107 31 93 62 428.45 23.87 108 31 97 66 420.97 27.61 109 31 98 67 420.50 27.71 110 31 99 68 420.16 27.56 111 32 35 3 830.44 1.41 112 32 36 4 826.30 2.68 113 32 97 65 468.57 32.08 114 33 36 3 842.01 5.20 115 33 97 64 480.35 26.59 116 35 38 3 852.21 0.10 117 35 39 4 841.80 2.86 118 36 39 3 855.65 1.61 119 36 40 4 840.74 2.32 120 36 93 57 519.32 39.62 121 36 94 58 520.57 38.99 122 36 97 61 519.35 39.62 123 37 40 3 849.75 1.12 124 37 41 4 841.81 2.85 125 38 41 3 851.41 0.06 126 38 42 4 832.08 5.29 127 39 42 3 836.51 7.51 128 39 43 4 832.08 5.29 129 39 93 54 537.64 35.67 130 39 99 60 533.56 37.64 131 39 100 61 525.82 41.51 132 40 43 3 852.08 0.28 133 40 44 4 832.07 5.29 134 40 90 50 537.63 35.69 135 40 93 53 537.77 35.62 136 40 94 54 538.15 35.62 137 41 44 3 845.50 1.43 138 41 45 4 830.88 5.87 139 42 45 3 849.95 0.80 140 42 46 4 830.97 5.82 141 42 99 57 538.20 35.66 142 42 100 58 538.19 35.66 143 42 101 59 538.18 35.66 144 42 141 99 491.31 13.94 145 43 46 3 848.58 1.56 146 43 47 4 835.44 8.05 147 43 86 43 538.56 35.55 148 43 89 46 538.52 35.53 149 43 90 47 538.61 35.49 150 43 93 50 539.06 35.28 151 43 100 57 538.19 35.66 152 43 137 94 492.78 13.94 153 43 141 98 492.05 13.58 154 44 47 3 848.50 1.53 155 44 48 4 835.40 8.04 156 44 86 42 539.02 35.33 157 44 90 46 539.12 35.27 158 45 48 3 847.13 2.18 159 45 49 4 847.18 2.19 160 46 49 3 849.85 1.67 161 46 50 4 836.15 7.71 162 46 137 91 493.78 13.44 163 46 140 94 493.25 13.19 164 46 141 95 493.06 13.10 165 47 50 3 849.85 1.67 166 47 51 4 836.09 7.67 167 47 80 33 539.14 35.41 168 47 81 34 539.17 35.39 169 47 86 39 544.72 32.50 170 47 137 90 493.95 13.37 171 48 51 3 854.80 4.14 172 48 52 4 840.24 5.59 173 49 52 3 847.98 7.55 174 49 53 4 831.76 5.50 175 49 140 91 494.19 12.80 176 50 53 3 854.98 4.05 177 50 54 4 831.77 5.51 178 50 80 30 544.90 32.61 179 50 136 86 494.68 13.09 180 50 137 87 494.72 13.06 181 50 140 90 494.55 12.98 182 51 54 3 834.15 4.36 183 51 55 4 835.97 3.42 184 51 80 29 544.91 32.60 185 51 81 30 545.01 32.52 186 52 55 3 845.50 1.32 187 52 56 4 842.70 0.08 188 53 56 3 850.69 1.26 189 53 57 4 836.20 3.32 190 53 136 83 501.21 10.60 191 53 140 87 500.66 10.13 192 53 146 93 494.68 7.50 193 54 57 3 847.51 0.33 194 54 58 4 835.99 3.42 195 54 73 19 546.56 32.58 196 54 76 22 546.49 32.56 197 54 77 23 546.40 32.52 198 54 80 26 546.39 32.53 199 54 133 79 502.05 10.75 200 54 136 82 501.69 10.57 201 55 58 3 849.62 3.18 202 55 59 4 836.21 3.31 203 55 77 22 546.48 32.56 204 56 59 3 838.17 4.30 205 56 60 4 827.38 3.48 206 57 60 3 836.63 5.07 207 57 61 4 820.34 0.21 208 57 73 16 546.71 32.55 209 57 136 79 502.32 10.67 210 57 166 109 466.13 6.77 211 57 169 112 465.99 6.79 212 57 170 113 465.83 6.85 213 58 61 3 833.79 6.50 214 58 62 4 818.55 0.69 215 58 70 12 546.78 32.59 216 58 73 15 546.71 32.55 217 58 74 16 546.72 32.55 218 58 77 19 546.71 32.55 219 59 62 3 818.53 0.70 220 59 63 4 818.49 0.70 221 60 64 4 800.45 22.87 222 60 148 88 494.75 7.38 223 60 150 90 494.12 7.23 224 60 170 110 465.91 6.81 225 61 64 3 844.43 0.98 226 61 65 4 818.11 4.11 227 61 66 5 752.97 11.19 228 61 69 8 564.33 26.76 229 61 73 12 546.84 32.60 230 61 169 108 466.06 6.78 231 62 65 3 843.11 0.33 232 62 66 4 748.59 9.37 233 62 70 8 546.85 32.61 234 64 173 109 466.00 6.83 235 65 69 4 781.19 14.25 236 65 168 103 466.50 6.90 237 65 169 104 466.42 6.88 238 65 173 108 466.12 6.78 239 65 193 128 319.92 1.34 240 66 69 3 849.23 1.62 241 66 70 4 827.86 1.02 242 67 70 3 827.87 1.02 243 67 71 4 822.11 1.84 244 68 71 3 854.76 3.97 245 69 72 3 847.52 2.08 246 69 73 4 838.58 4.33 247 69 189 120 320.00 1.35 248 70 73 3 848.31 2.47 249 70 74 4 838.38 4.43 250 71 74 3 848.57 7.02 251 72 75 3 859.83 1.40 252 72 76 4 846.19 0.53 253 72 165 93 466.49 6.92 254 73 76 3 844.51 0.32 255 73 77 4 844.50 0.32 256 73 165 92 466.47 6.92 257 73 166 93 466.47 6.92 258 73 169 96 466.31 6.85 259 74 77 3 849.28 2.07 260 74 78 4 835.64 4.11 261 75 78 3 836.99 4.79 262 75 79 4 822.62 3.36 263 76 79 3 850.50 1.36 264 76 80 4 826.25 1.54 265 76 129 53 502.82 10.41 266 76 133 57 502.34 10.65 267 77 80 3 847.22 1.06 268 77 81 4 800.67 14.28 269 78 82 4 772.90 15.54 270 79 82 3 834.74 7.12 271 79 85 6 738.70 27.85 272 79 130 51 502.87 10.40 273 79 133 54 502.82 10.40 274 80 133 53 502.75 10.38 275 80 137 57 501.09 9.95 276 82 85 3 831.75 1.44 277 82 86 4 823.56 2.67 278 83 86 3 854.95 4.40 279 83 87 4 838.69 1.54 280 84 87 3 844.36 1.29 281 84 88 4 838.69 1.54 282 85 88 3 857.45 3.16 283 85 89 4 841.78 0.01 284 85 107 22 550.91 29.32 285 85 108 23 550.92 29.33 286 85 111 26 550.88 29.31 287 86 89 3 848.93 1.10 288 86 90 4 841.77 0.01 289 87 90 3 847.02 0.26 290 87 91 4 832.42 4.69 291 88 91 3 846.57 0.39 292 88 92 4 836.35 2.73 293 88 107 19 551.04 29.32 294 89 92 3 858.52 2.54 295 89 93 4 848.72 3.48 296 89 103 14 551.09 29.34 297 89 104 15 551.07 29.34 298 89 107 18 551.04 29.32 299 90 93 3 844.40 2.21 300 90 94 4 832.39 4.67 301 91 94 3 846.74 3.35 302 91 95 4 832.22 4.76 303 91 96 5 832.21 4.76 304 92 96 4 733.14 16.59 305 92 97 5 731.15 17.59 306 92 98 6 687.29 35.19 307 92 103 11 567.42 21.18 308 92 106 14 564.80 22.48 309 92 107 15 564.80 22.49 310 93 97 4 726.86 15.44 311 93 98 5 687.32 35.17 312 93 100 7 624.99 4.03 313 93 103 10 581.12 18.16 314 94 97 3 846.43 5.15 315 96 106 10 685.41 24.28 316 98 102 4 820.32 3.73 317 98 103 5 739.34 18.47 318 98 106 8 721.78 9.71 319 99 102 3 843.16 0.74 320 99 103 4 826.63 0.58 321 100 103 3 849.01 2.18 322 100 104 4 826.63 0.57 323 100 141 41 508.24 5.51 324 101 105 4 844.99 4.19 325 101 138 37 508.88 5.83 326 101 141 40 508.25 5.51 327 101 142 41 508.23 5.51 328 102 105 3 857.66 0.55 329 102 106 4 842.20 2.79 330 103 106 3 853.12 1.72 331 103 107 4 849.18 0.26 332 104 107 3 859.65 5.49 333 104 108 4 831.12 2.76 334 104 137 33 509.34 5.66 335 104 138 34 509.33 5.65 336 104 141 37 508.66 5.32 337 105 108 3 856.40 3.67 338 105 109 4 824.78 0.40 339 105 138 33 509.32 5.64 340 106 109 3 841.47 2.40 341 106 110 4 831.00 2.83 342 107 110 3 834.58 4.62 343 107 111 4 837.03 3.39 344 108 111 3 839.50 4.61 345 108 112 4 830.91 6.45 346 108 130 22 511.38 6.08 347 108 134 26 509.44 5.70 348 109 112 3 849.37 0.91 349 109 113 4 827.49 4.72 350 109 131 22 511.37 6.08 351 110 113 3 860.45 0.99 352 110 114 4 846.62 1.09 353 111 114 3 855.74 3.35 354 111 115 4 855.62 3.40 355 111 130 19 511.50 6.10 356 112 115 3 860.79 0.82 357 112 116 4 846.63 1.09 358 112 127 15 511.49 6.11 359 112 130 18 511.50 6.09 360 112 131 19 511.49 6.11 361 113 116 3 846.78 1.02 362 113 131 18 511.51 6.10 363 114 117 3 848.82 0.11 364 115 123 8 511.84 6.08 365 115 126 11 511.84 6.08 366 115 127 12 511.79 6.08 367 115 130 15 511.78 6.08 368 116 127 11 511.81 6.08 369 116 131 15 511.81 6.08 370 118 123 5 764.02 24.75 371 119 124 5 814.45 0.16 372 120 123 3 837.63 4.66 373 120 124 4 820.86 2.56 374 121 124 3 834.07 2.88 375 121 125 4 816.48 0.37 376 122 125 3 831.54 7.91 377 122 126 4 820.97 2.62 378 123 126 3 847.05 0.92 379 123 127 4 826.71 0.26 380 124 127 3 839.54 0.67 381 124 128 4 822.23 2.50 382 125 128 3 827.14 0.04 383 125 129 4 831.72 2.25 384 126 129 3 838.80 1.29 385 126 130 4 829.40 5.99 386 127 130 3 836.86 2.26 387 127 131 4 834.11 3.64 388 128 131 3 847.00 5.37 389 128 132 4 827.78 4.25 390 129 132 3 840.94 0.62 391 129 133 4 843.60 0.67 392 129 165 36 466.48 6.94 393 130 133 3 850.07 3.90 394 130 134 4 843.60 0.66 395 131 134 3 844.78 1.25 396 131 135 4 836.91 2.69 397 132 135 3 845.00 1.35 398 132 136 4 836.93 2.68 399 132 164 32 466.47 6.96 400 132 166 34 466.47 6.96 401 133 136 3 845.56 0.86 402 133 137 4 836.93 2.68 403 134 137 3 846.88 0.98 404 134 138 4 841.97 0.16 405 135 138 3 851.98 5.17 406 135 139 4 841.87 0.11 407 136 139 3 857.62 2.02 408 136 140 4 835.11 4.01 409 136 146 10 512.53 0.65 410 136 166 30 466.44 6.97 411 137 140 3 857.62 2.02 412 137 141 4 823.23 0.79 413 138 141 3 844.38 8.64 414 138 142 4 821.08 1.84 415 139 142 3 833.88 3.81 416 139 143 4 825.87 0.58 417 139 144 5 691.43 11.91 418 139 146 7 605.72 11.65 419 140 143 3 822.46 2.27 420 140 146 6 619.88 18.73 421 147 170 23 467.26 7.57 422 148 151 3 863.86 2.45 423 148 170 22 467.18 7.61 424 149 152 3 881.85 4.03 425 149 167 18 467.28 7.38 426 149 170 21 467.18 7.61 427 149 171 22 467.10 7.65 428 150 170 20 467.19 7.62 429 150 172 22 478.39 13.29 430 152 156 4 854.51 0.71 431 152 172 20 615.27 81.66 432 152 175 23 631.43 89.83 433 153 175 22 632.12 90.14 434 154 175 21 632.13 90.15 435 155 175 20 632.12 90.14 436 155 177 22 632.08 90.17 437 156 175 19 632.21 90.18 438 156 176 20 632.14 90.20 439 156 177 21 632.14 90.20 440 157 177 20 631.26 90.64 441 157 178 21 631.19 90.62 442 157 180 23 621.32 95.53 443 157 218 61 119.08 1.02 444 157 220 63 117.25 1.31 445 158 163 5 838.88 1.80 446 158 171 13 632.63 90.01 447 158 176 18 631.87 90.36 448 158 178 20 631.25 90.65 449 158 179 21 610.75 100.86 450 158 180 22 598.43 106.99 451 159 180 21 591.13 110.64 452 159 208 49 247.05 1.90 453 159 209 50 247.02 1.90 454 160 163 3 919.88 0.17 455 160 180 20 587.86 112.34 456 160 181 21 587.79 112.34 457 160 182 22 587.77 112.36 458 160 209 49 246.56 1.66 459 161 181 20 577.28 117.62 460 161 182 21 577.20 117.64 461 161 183 22 577.14 117.67 462 161 209 48 246.56 1.66 463 162 181 19 577.25 117.64 464 162 183 21 565.00 123.70 465 162 190 28 319.92 1.34 466 163 167 4 825.66 5.18 467 163 168 5 805.55 4.88 468 163 171 8 754.96 29.05 469 163 179 16 587.91 112.38 470 163 181 18 577.29 117.63 471 163 190 27 319.58 1.43 472 164 167 3 838.45 1.22 473 164 168 4 825.67 5.17 474 164 189 25 319.75 1.33 475 165 168 3 841.75 0.45 476 165 169 4 827.93 4.04 477 165 189 24 319.71 1.43 478 166 170 4 841.20 1.81 479 167 170 3 853.82 1.57 480 167 171 4 813.86 8.98 481 168 171 3 838.34 3.24 482 168 189 21 319.88 1.39 483 168 193 25 319.36 1.59 484 171 176 5 763.13 25.00 485 171 179 8 601.28 105.78 486 171 193 22 319.86 1.38 487 172 175 3 861.58 1.21 488 172 176 4 822.56 5.77 489 173 176 3 841.12 0.03 490 173 193 20 320.00 1.39 491 173 197 24 317.94 2.12 492 174 197 23 319.77 1.42 493 176 199 23 317.56 1.94 494 177 200 23 317.28 1.97 495 178 199 21 317.29 1.96 496 178 200 22 317.22 1.94 497 178 220 42 117.25 1.31 498 179 190 11 320.18 1.46 499 179 193 14 320.12 1.44 500 179 194 15 319.96 1.36 501 179 199 20 317.33 1.97 502 179 200 21 317.20 1.93 503 179 201 22 316.28 1.76 504 179 202 23 316.29 1.76 505 179 220 41 117.26 1.31 506 180 202 22 316.28 1.76 507 180 205 25 315.83 1.82 508 180 208 28 247.46 1.72 509 180 218 38 119.44 1.23 510 180 220 40 117.28 1.31 511 180 227 47 112.83 0.19 512 181 202 21 316.28 1.76 513 181 203 22 315.98 1.79 514 181 205 24 303.15 0.76 515 181 208 27 267.18 8.17 516 182 203 21 316.16 1.81 517 182 205 23 301.00 0.18 518 182 206 24 291.91 2.54 519 182 207 25 287.31 0.26 520 182 208 26 285.68 1.07 521 182 209 27 271.39 6.03 522 183 203 20 316.25 1.77 523 184 203 19 316.33 1.73 524 185 188 3 807.94 2.48 525 185 191 6 622.90 10.97 526 185 203 18 316.25 1.77 527 186 191 5 643.85 21.30 528 186 195 9 643.95 21.22 529 186 203 17 316.35 1.73 530 187 191 4 775.18 6.30 531 187 192 5 664.58 31.40 532 187 195 8 644.70 21.38 533 188 191 3 839.82 0.65 534 188 192 4 816.01 0.82 535 189 193 4 818.99 0.68 536 190 193 3 826.70 2.69 537 190 194 4 819.00 0.68 538 190 203 13 316.30 1.75 539 191 194 3 840.09 4.00 540 191 195 4 829.81 0.98 541 191 203 12 316.31 1.75 542 192 195 3 850.66 1.29 543 192 196 4 829.82 0.98 544 193 196 3 851.27 1.58 545 193 197 4 829.83 0.96 546 194 197 3 829.84 0.96 547 194 198 4 807.44 12.17 548 194 199 5 627.76 34.09 549 194 201 7 316.93 1.99 550 195 198 3 829.82 0.96 551 200 220 20 116.88 1.31 552 200 222 22 112.92 0.23 553 202 205 3 904.06 1.40 554 202 220 18 117.25 1.32 555 202 222 20 113.21 0.32 556 202 225 23 112.82 0.19 557 205 208 3 875.21 1.97 558 207 210 3 849.43 7.02 559 207 211 4 782.82 10.50 560 208 211 3 824.70 4.90 561 208 212 4 735.47 34.16 562 208 227 19 112.95 0.23 563 212 216 4 799.58 14.80 564 212 218 6 470.07 51.59 565 212 229 17 112.83 0.20 566 213 216 3 862.13 1.28 567 216 229 13 112.83 0.18 568 216 230 14 112.52 0.06 569 217 230 13 112.45 0.03 570 217 231 14 112.45 0.03 571 217 236 19 77.34 0.32 572 218 227 9 112.87 0.17 573 218 228 10 112.55 0.04 574 218 229 11 112.47 0.03 575 218 230 12 110.68 0.85 576 219 227 8 112.70 0.10 577 219 228 9 112.53 0.04 578 219 231 12 109.08 1.24 579 219 239 20 33.98 0.82 580 220 226 6 123.48 5.48 581 220 227 7 112.48 0.02 582 221 224 3 858.26 4.07 583 221 225 4 607.21 16.23 584 221 226 5 123.46 5.48 585 221 228 7 112.48 0.01 586 221 239 18 33.92 0.82 587 222 225 3 810.11 10.52 588 223 243 20 31.57 0.14 589 224 243 19 31.57 0.15 590 228 231 3 869.69 7.70 591 228 235 7 631.27 20.18 592 228 239 11 187.38 33.80 593 231 235 4 759.83 26.46 594 231 236 5 711.74 2.43 595 232 235 3 823.71 3.94 596 232 236 4 814.31 0.76 597 233 236 3 817.63 0.90 598 233 237 4 814.33 0.74 599 233 240 7 703.43 1.72 600 234 237 3 822.01 4.58 601 234 238 4 814.33 0.74 602 235 238 3 830.41 2.69 603 235 239 4 817.85 1.70 604 236 239 3 845.08 3.63 605 236 240 4 774.60 18.79 606 237 240 3 834.50 0.27 607 238 241 3 885.22 0.44 608 238 242 4 762.01 9.01 609 238 243 5 743.30 18.37 610 239 242 3 834.99 8.21 611 239 243 4 688.75 45.64 612 239 244 5 645.88 26.83 613 240 244 4 703.32 1.87 614 241 244 3 760.77 26.87 615 241 245 4 645.88 26.83 616 243 246 3 853.18 0.81 617 245 248 3 867.70 3.12 618 246 249 3 854.62 9.67