BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 9 8 788.91 7.83 2 2 5 3 831.73 0.80 3 2 6 4 812.47 6.02 4 3 6 3 830.69 1.31 5 3 7 4 813.58 2.97 6 4 7 3 826.42 0.95 7 4 8 4 815.87 1.83 8 5 8 3 832.80 2.23 9 5 9 4 823.14 2.59 10 6 9 3 833.85 1.84 11 6 10 4 823.13 2.59 12 7 10 3 836.37 0.58 13 7 11 4 823.16 2.57 14 7 127 120 36.12 1.49 15 8 11 3 837.23 0.14 16 8 123 115 24.58 0.76 17 9 172 163 98.59 0.01 18 10 172 162 98.60 0.01 19 11 122 111 41.00 0.00 20 11 126 115 41.06 0.03 21 11 172 161 98.66 0.04 22 11 175 164 98.70 0.01 23 12 169 157 98.79 0.07 24 12 170 158 98.92 0.00 25 12 171 159 98.84 0.04 26 12 172 160 98.66 0.04 27 13 132 119 41.18 0.08 28 13 133 120 41.06 0.03 29 13 134 121 41.07 0.03 30 14 17 3 912.85 0.68 31 14 131 117 41.57 0.01 32 14 132 118 41.37 0.03 33 14 133 119 41.20 0.09 34 14 166 152 96.42 0.54 35 14 168 154 99.08 0.01 36 14 169 155 99.09 0.00 37 14 170 156 99.07 0.01 38 15 69 54 70.50 1.18 39 15 133 118 62.10 0.68 40 16 72 56 98.33 0.13 41 16 166 150 99.09 0.01 42 16 167 151 99.14 0.01 43 16 168 152 99.84 0.15 44 17 69 52 98.45 0.07 45 17 72 55 98.33 0.13 46 17 73 56 98.33 0.13 47 17 131 114 99.06 0.03 48 17 132 115 99.01 0.01 49 17 133 116 98.94 0.01 50 17 162 145 98.59 0.01 51 17 164 147 98.91 0.01 52 17 165 148 99.07 0.02 53 17 166 149 99.09 0.01 54 17 167 150 99.64 0.25 55 18 165 147 99.14 0.01 56 18 166 148 100.00 0.19 57 18 167 149 101.05 0.34 58 20 74 54 102.92 0.21 59 20 165 145 103.27 0.04 60 21 74 53 103.42 0.04 61 21 165 144 104.56 0.34 62 22 26 4 819.38 6.31 63 22 27 5 809.35 1.30 64 22 74 52 104.34 0.29 65 22 75 53 104.23 0.27 66 22 130 108 104.56 0.35 67 22 163 141 104.25 0.26 68 22 164 142 104.27 0.25 69 22 165 143 104.64 0.32 70 23 26 3 828.41 1.79 71 23 27 4 825.97 3.02 72 24 27 3 825.98 3.01 73 24 28 4 825.99 3.00 74 25 28 3 826.00 3.00 75 25 29 4 826.00 3.00 76 26 29 3 833.58 3.62 77 26 30 4 816.71 1.65 78 26 128 102 119.62 0.44 79 26 130 104 119.62 0.45 80 27 30 3 822.55 3.05 81 27 31 4 820.09 4.28 82 27 50 23 117.98 0.03 83 27 52 25 120.43 0.01 84 27 75 48 119.61 0.42 85 28 31 3 835.06 3.21 86 28 32 4 815.41 2.24 87 29 32 3 824.09 6.21 88 29 33 4 816.87 2.97 89 29 128 99 127.63 0.40 90 30 33 3 831.61 2.45 91 30 34 4 820.53 1.14 92 30 121 91 119.59 0.42 93 30 124 94 119.63 0.42 94 30 125 95 124.25 0.64 95 30 128 98 127.65 0.40 96 30 130 100 127.77 0.36 97 30 163 133 127.48 0.33 98 31 34 3 823.48 2.55 99 31 35 4 820.53 1.14 100 31 48 17 120.40 0.01 101 32 35 3 830.64 1.02 102 32 36 4 817.55 0.35 103 33 36 3 816.34 0.96 104 33 37 4 816.34 0.96 105 33 120 87 126.73 0.04 106 33 124 91 127.58 0.36 107 34 37 3 819.42 1.96 108 34 48 14 127.49 0.33 109 34 79 45 127.49 0.33 110 34 117 83 124.19 1.18 111 34 120 86 126.90 0.06 112 34 121 87 127.48 0.33 113 37 116 79 127.61 0.35 114 37 120 83 127.69 0.35 115 38 116 78 127.70 0.35 116 39 42 3 818.30 3.78 117 39 43 4 769.40 4.36 118 39 44 5 517.13 5.89 119 39 113 74 127.55 0.32 120 40 43 3 842.62 0.66 121 40 45 5 715.64 22.53 122 40 109 69 126.83 0.14 123 40 113 73 127.78 0.38 124 44 85 41 137.50 2.77 125 45 81 36 139.14 3.51 126 45 82 37 135.78 3.24 127 45 83 38 135.78 3.24 128 45 109 64 134.24 1.28 129 45 110 65 137.31 2.65 130 45 113 68 139.15 3.50 131 46 79 33 139.47 3.56 132 46 80 34 139.41 3.58 133 46 81 35 139.16 3.51 134 46 82 36 139.15 3.52 135 46 85 39 139.42 3.58 136 46 113 67 139.20 3.51 137 46 114 68 139.38 3.58 138 46 117 71 139.47 3.56 139 47 78 31 140.47 3.48 140 47 79 32 140.07 3.32 141 47 80 33 139.44 3.57 142 47 82 35 139.43 3.58 143 47 85 38 139.44 3.58 144 48 77 29 140.94 3.60 145 48 78 30 140.47 3.49 146 48 79 31 140.31 3.43 147 49 77 28 140.97 3.61 148 49 78 29 140.47 3.49 149 49 80 31 140.47 3.48 150 49 149 100 140.52 3.52 151 49 151 102 140.48 3.50 152 50 75 25 144.59 2.71 153 50 76 26 141.81 3.50 154 50 77 27 140.97 3.61 155 51 56 5 785.04 0.24 156 51 57 6 692.45 13.17 157 51 60 9 615.32 25.40 158 51 75 24 150.12 0.04 159 51 76 25 144.40 2.62 160 51 149 98 141.68 3.90 161 52 74 22 195.73 0.13 162 52 75 23 161.81 10.52 163 53 56 3 828.96 0.08 164 53 57 4 817.29 1.95 165 54 57 3 830.19 5.12 166 54 58 4 817.33 1.97 167 54 71 17 213.70 0.84 168 55 58 3 836.50 7.62 169 55 59 4 817.35 1.98 170 56 59 3 842.57 4.58 171 56 60 4 823.37 5.01 172 56 148 92 205.05 1.37 173 56 149 93 205.05 1.37 174 57 60 3 830.04 1.69 175 57 61 4 829.98 1.71 176 57 70 13 221.97 1.83 177 57 71 14 222.00 1.84 178 57 73 16 221.96 1.83 179 57 74 17 221.88 1.83 180 57 75 18 221.40 1.79 181 57 76 19 214.28 0.55 182 58 61 3 828.97 1.64 183 58 62 4 823.94 1.30 184 58 71 13 221.97 1.82 185 59 62 3 828.96 1.64 186 59 63 4 826.78 2.73 187 59 148 89 221.32 1.78 188 60 63 3 829.09 1.75 189 60 64 4 813.46 2.58 190 60 70 10 461.25 12.08 191 60 76 16 221.50 1.83 192 60 145 85 221.30 1.79 193 60 148 88 221.38 1.79 194 60 149 89 221.37 1.78 195 61 64 3 812.03 4.54 196 61 65 4 809.88 4.37 197 61 66 5 798.24 1.03 198 61 67 6 762.96 18.65 199 61 70 9 413.73 35.84 200 61 71 10 404.00 16.55 201 62 65 3 829.37 5.49 202 62 66 4 798.22 1.02 203 63 141 78 221.40 1.84 204 63 144 81 221.47 1.83 205 63 145 82 221.47 1.83 206 63 148 85 221.47 1.83 207 64 67 3 869.22 0.20 208 64 70 6 790.64 7.61 209 64 135 71 221.47 1.84 210 64 137 73 221.41 1.85 211 67 70 3 841.60 1.42 212 67 135 68 221.99 1.77 213 67 137 70 221.81 1.81 214 68 71 3 822.58 1.30 215 68 72 4 807.08 4.24 216 69 72 3 810.71 2.43 217 69 73 4 804.61 5.47 218 69 133 64 223.47 1.36 219 69 135 66 222.92 1.62 220 69 162 93 223.47 1.36 221 70 73 3 838.77 6.84 222 70 76 6 450.80 4.29 223 70 135 65 223.48 1.36 224 70 162 92 223.56 1.41 225 73 162 89 312.94 0.93 226 73 163 90 312.61 1.10 227 73 164 91 312.18 0.90 228 74 163 89 314.01 0.58 229 74 164 90 313.09 0.92 230 75 162 87 317.77 1.14 231 75 163 88 314.63 0.28 232 76 135 59 319.23 1.60 233 76 138 62 321.98 0.55 234 76 160 84 319.10 1.67 235 76 161 85 317.90 1.10 236 76 162 86 317.78 1.14 237 77 160 83 319.23 1.60 238 77 161 84 319.10 1.67 239 77 163 86 318.48 1.39 240 78 138 60 322.10 0.48 241 78 145 67 323.56 0.21 242 78 149 71 323.76 0.18 243 78 151 73 323.91 0.23 244 78 157 79 322.49 0.70 245 78 159 81 321.95 0.56 246 78 160 82 319.24 1.60 247 79 117 38 320.88 1.25 248 79 118 39 319.58 1.89 249 79 121 42 319.53 1.86 250 79 158 79 322.40 0.74 251 79 159 80 322.02 0.58 252 79 161 82 320.72 1.13 253 80 151 71 324.10 0.14 254 80 155 75 323.82 0.22 255 80 156 76 323.01 0.60 256 80 157 77 322.63 0.67 257 80 158 78 322.55 0.70 258 81 110 29 322.57 0.81 259 81 155 74 323.87 0.24 260 81 156 75 323.70 0.27 261 81 158 77 322.68 0.64 262 82 85 3 885.70 2.64 263 82 154 72 325.81 0.01 264 82 155 73 323.88 0.24 265 83 86 3 819.48 0.47 266 83 87 4 813.53 2.50 267 83 98 15 327.22 0.05 268 83 100 17 326.16 0.07 269 83 101 18 326.08 0.09 270 83 106 23 325.95 0.03 271 83 109 26 325.88 0.01 272 83 110 27 325.35 0.02 273 83 154 71 325.88 0.03 274 83 245 162 306.36 0.82 275 84 87 3 826.56 0.77 276 84 88 4 812.80 2.88 277 84 152 68 326.06 0.01 278 84 153 69 326.06 0.00 279 84 154 70 326.00 0.01 280 84 155 71 325.93 0.01 281 85 88 3 823.83 2.48 282 85 89 4 817.77 0.40 283 86 89 3 822.94 2.93 284 86 90 4 817.77 0.40 285 86 98 12 327.33 0.05 286 87 90 3 826.14 4.54 287 87 91 4 806.52 4.92 288 87 92 5 795.57 0.55 289 87 98 11 327.33 0.06 290 87 154 67 326.11 0.03 291 88 92 4 786.41 2.40 292 88 93 5 786.41 2.40 293 89 93 4 786.36 2.40 294 89 94 5 761.13 10.21 295 90 94 4 747.46 17.05 296 90 95 5 747.44 17.05 297 90 96 6 661.91 20.59 298 90 97 7 620.79 0.04 299 90 98 8 452.40 1.59 300 91 95 4 708.08 2.48 301 91 96 5 707.96 2.43 302 97 102 5 847.47 3.37 303 98 101 3 880.39 3.73 304 98 154 56 516.74 20.87 305 99 102 3 836.70 1.30 306 100 103 3 854.83 3.56 307 100 105 5 768.06 10.54 308 100 106 6 682.74 7.86 309 101 106 5 684.95 6.77 310 101 154 53 526.09 16.20 311 101 244 143 306.44 0.83 312 101 245 144 306.39 0.83 313 101 246 145 306.38 0.82 314 102 246 144 306.36 0.83 315 102 248 146 306.31 0.83 316 103 106 3 850.43 1.18 317 103 246 143 306.38 0.83 318 103 248 145 303.26 0.04 319 104 107 3 834.66 2.71 320 104 108 4 816.51 2.50 321 104 247 143 306.09 0.89 322 104 248 144 303.26 0.04 323 105 108 3 828.16 0.41 324 105 109 4 821.81 0.15 325 106 109 3 823.08 0.48 326 106 110 4 821.82 0.14 327 106 245 139 306.38 0.83 328 107 110 3 823.88 0.08 329 107 111 4 826.11 2.29 330 107 156 49 612.55 2.10 331 107 240 133 306.78 0.77 332 107 245 138 306.36 0.83 333 108 111 3 826.11 2.29 334 108 112 4 809.98 0.74 335 109 112 3 842.34 2.67 336 109 113 4 815.06 3.25 337 110 113 3 828.06 2.34 338 110 114 4 809.25 2.00 339 110 158 48 613.12 1.87 340 111 114 3 825.21 1.08 341 111 115 4 802.27 1.54 342 111 158 47 613.07 1.85 343 112 115 3 825.20 1.08 344 112 116 4 817.67 4.83 345 113 116 3 824.89 1.24 346 113 117 4 817.67 4.83 347 114 117 3 824.97 1.19 348 114 118 4 814.78 3.44 349 114 158 44 613.35 1.95 350 115 118 3 821.30 3.00 351 115 119 4 814.88 3.38 352 116 119 3 830.67 0.17 353 116 120 4 818.39 1.53 354 117 120 3 823.18 3.92 355 117 121 4 823.16 3.92 356 118 121 3 823.18 3.92 357 118 122 4 823.17 3.92 358 118 134 16 613.65 2.02 359 118 159 41 613.64 2.00 360 118 160 42 613.63 2.04 361 118 161 43 613.62 2.04 362 119 122 3 819.48 4.94 363 119 123 4 818.84 5.25 364 120 123 3 826.50 1.43 365 120 124 4 821.15 6.41 366 121 124 3 828.81 2.58 367 121 125 4 813.87 2.77 368 121 161 40 613.74 2.05 369 122 125 3 832.32 0.83 370 122 126 4 813.88 2.77 371 122 132 10 613.69 2.06 372 122 161 39 613.75 2.06 373 123 126 3 836.73 3.04 374 123 127 4 821.50 0.92 375 124 127 3 833.99 0.03 376 124 128 4 826.90 3.52 377 125 128 3 831.12 0.32 378 125 129 4 822.15 1.15 379 125 130 5 755.81 1.56 380 125 132 7 615.77 1.09 381 125 163 38 613.78 2.08 382 126 129 3 817.41 2.96 383 126 130 4 802.32 0.89 384 126 132 6 736.07 26.55 385 129 166 37 613.69 2.08 386 129 169 40 613.26 2.23 387 130 163 33 614.02 2.02 388 130 164 34 613.96 2.00 389 130 165 35 613.70 2.08 390 130 166 36 613.69 2.09 391 131 163 32 614.05 2.02 392 131 164 33 614.01 2.02 393 131 166 35 613.61 2.12 394 132 161 29 614.20 2.00 395 132 162 30 614.10 2.00 396 132 163 31 614.07 2.01 397 133 161 28 614.23 2.01 398 133 162 29 614.12 2.02 399 134 159 25 614.56 2.04 400 134 160 26 614.40 2.03 401 134 161 27 614.23 2.01 402 135 138 3 860.10 3.42 403 135 159 24 614.56 2.04 404 135 160 25 614.48 2.00 405 135 162 27 614.36 2.00 406 136 139 3 858.25 2.81 407 136 159 23 614.57 2.04 408 137 140 3 827.78 1.41 409 137 233 96 308.04 0.49 410 138 142 4 812.19 2.31 411 138 145 7 641.15 11.86 412 138 157 19 614.95 2.00 413 138 158 20 614.86 2.03 414 138 159 21 614.70 2.02 415 138 160 22 614.68 2.01 416 139 142 3 818.81 0.99 417 139 157 18 614.95 2.00 418 139 158 19 614.94 1.99 419 139 233 94 308.04 0.49 420 139 235 96 308.00 0.48 421 139 236 97 306.74 0.77 422 140 233 93 308.04 0.49 423 140 235 95 308.02 0.49 424 141 144 3 846.28 7.90 425 141 145 4 810.91 1.68 426 142 145 3 826.27 2.43 427 142 146 4 819.33 5.90 428 142 157 15 615.01 2.02 429 142 235 93 308.00 0.48 430 142 239 97 306.69 0.76 431 143 146 3 826.28 2.43 432 143 147 4 822.90 4.12 433 143 242 99 306.60 0.75 434 144 147 3 826.29 2.44 435 144 148 4 820.47 5.34 436 145 148 3 822.91 4.12 437 145 149 4 820.46 5.34 438 145 157 12 615.07 2.00 439 146 149 3 827.15 2.56 440 146 150 4 798.63 12.30 441 146 151 5 703.81 5.51 442 146 157 11 615.06 2.00 443 146 239 93 306.74 0.77 444 146 243 97 306.43 0.83 445 147 150 3 818.57 5.57 446 151 155 4 719.15 10.35 447 151 157 6 615.38 1.96 448 152 155 3 828.63 0.18 449 152 243 91 306.44 0.83 450 153 243 90 306.44 0.83 451 153 244 91 306.43 0.83 452 154 243 89 306.43 0.83 453 154 244 90 306.39 0.83 454 154 245 91 306.36 0.82 455 155 243 88 306.43 0.83 456 155 245 90 306.36 0.82 457 156 239 83 306.72 0.79 458 156 240 84 306.55 0.83 459 156 243 87 306.40 0.83 460 156 245 89 306.26 0.82 461 157 239 82 306.72 0.78 462 166 169 3 835.64 7.16 463 170 211 41 421.38 2.06 464 171 211 40 421.44 2.08 465 172 207 35 421.76 2.01 466 173 204 31 421.77 2.00 467 173 207 34 421.77 2.01 468 174 200 26 422.68 1.92 469 174 204 30 421.78 2.00 470 174 207 33 421.78 2.00 471 175 200 25 422.70 1.91 472 176 180 4 828.12 1.68 473 176 181 5 811.19 2.25 474 176 197 21 422.72 1.91 475 176 200 24 422.78 1.88 476 176 220 44 422.69 1.91 477 177 180 3 834.93 1.80 478 177 181 4 831.40 0.04 479 177 196 19 423.11 1.93 480 177 200 23 423.03 1.92 481 177 203 26 423.12 1.94 482 178 181 3 836.63 2.29 483 178 182 4 812.91 3.08 484 179 182 3 836.63 2.29 485 179 183 4 825.19 3.06 486 179 188 9 430.49 0.69 487 179 196 17 423.36 1.85 488 180 183 3 884.78 1.06 489 180 188 8 424.94 2.08 490 180 193 13 423.34 1.84 491 180 196 16 423.34 1.83 492 183 186 3 857.85 2.41 493 183 187 4 634.94 24.69 494 183 188 5 430.91 4.14 495 183 193 10 430.90 4.15 496 187 193 6 809.56 1.50 497 188 192 4 821.30 0.27 498 188 193 5 804.33 4.11 499 188 196 8 781.59 0.33 500 189 192 3 848.00 4.22 501 189 193 4 818.37 1.20 502 190 193 3 820.93 0.08 503 190 194 4 818.38 1.20 504 191 194 3 827.87 3.38 505 191 195 4 820.86 0.05 506 192 195 3 820.88 0.06 507 192 196 4 823.58 1.29 508 193 196 3 828.55 3.78 509 193 197 4 823.60 1.30 510 194 197 3 829.64 3.23 511 194 198 4 823.60 1.30 512 195 198 3 824.62 0.96 513 195 199 4 823.59 1.31 514 196 199 3 823.60 1.30 515 196 200 4 823.12 1.54 516 197 200 3 822.20 1.77 517 197 201 4 811.55 4.23 518 197 220 23 426.59 0.77 519 197 221 24 426.51 0.79 520 198 201 3 828.25 4.67 521 198 202 4 814.36 5.67 522 199 202 3 822.32 1.70 523 199 203 4 811.48 4.24 524 200 203 3 823.60 3.23 525 200 204 4 817.97 0.99 526 200 220 20 426.75 0.72 527 201 204 3 818.47 0.73 528 201 205 4 812.25 1.88 529 201 219 18 426.69 0.76 530 201 220 19 426.67 0.75 531 201 221 20 426.67 0.76 532 202 205 3 819.88 1.44 533 202 206 4 812.24 1.88 534 203 206 3 824.87 0.69 535 203 207 4 817.73 0.83 536 204 207 3 819.07 0.21 537 204 208 4 822.83 1.71 538 204 219 15 426.91 0.67 539 204 220 16 426.88 0.67 540 205 208 3 823.52 1.38 541 205 209 4 822.92 1.68 542 205 217 12 427.12 0.64 543 205 219 14 426.94 0.68 544 206 209 3 823.52 1.38 545 206 210 4 822.94 1.67 546 207 210 3 838.24 0.63 547 207 211 4 821.81 1.10 548 208 211 3 821.82 1.10 549 208 212 4 821.81 1.10 550 208 216 8 439.04 5.73 551 208 217 9 428.53 0.52 552 208 218 10 428.44 0.56 553 209 212 3 821.82 1.10 554 209 213 4 772.75 15.09 555 209 214 5 644.12 3.62 556 209 216 7 439.43 5.55 557 209 217 8 428.51 0.52 558 210 213 3 787.91 7.50 559 212 215 3 811.51 1.99 560 212 216 4 683.72 42.04 561 218 222 4 798.35 3.69 562 218 223 5 776.41 0.27 563 219 222 3 822.54 0.17 564 219 223 4 819.51 1.69 565 220 223 3 819.55 1.67 566 220 224 4 819.51 1.69 567 221 224 3 823.09 0.56 568 221 225 4 798.35 3.71 569 222 225 3 827.39 2.43 570 222 226 4 819.00 6.61 571 223 226 3 827.34 2.45 572 223 227 4 823.93 4.15 573 224 227 3 826.86 3.93 574 224 228 4 804.89 5.38 575 225 229 4 801.04 3.46 576 226 229 3 855.62 3.04 577 227 230 3 878.24 2.39 578 227 232 5 768.62 0.45 579 228 232 4 812.91 4.09 580 231 234 3 842.55 0.14 581 233 236 3 848.95 1.22 582 235 238 3 817.84 0.48 583 235 239 4 803.49 4.09 584 236 239 3 827.39 5.25 585 236 240 4 812.89 0.61 586 237 240 3 823.82 4.75 587 237 241 4 812.35 0.88 588 237 247 10 374.43 28.59 589 238 241 3 824.74 4.29 590 238 242 4 812.34 0.88 591 239 242 3 814.94 0.41 592 239 243 4 812.32 0.87 593 240 243 3 818.55 1.46 594 240 244 4 812.31 0.88 595 240 245 5 659.13 54.45 596 241 244 3 812.20 0.92 597 241 245 4 789.20 10.57