BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 41 40 71.94 0.60 2 1 42 41 75.20 1.03 3 1 95 94 65.56 0.27 4 2 25 23 83.70 0.83 5 2 26 24 83.89 0.74 6 2 39 37 83.59 0.89 7 2 40 38 82.92 1.18 8 2 41 39 79.75 0.42 9 2 42 40 78.53 0.61 10 3 25 22 84.25 0.71 11 3 26 23 84.28 0.70 12 3 27 24 84.31 0.72 13 3 28 25 84.55 0.61 14 3 40 37 83.59 0.89 15 3 42 39 81.46 0.86 16 4 28 24 85.03 0.38 17 4 30 26 85.44 0.53 18 4 37 33 84.06 0.70 19 4 40 36 84.05 0.69 20 4 42 38 83.81 0.78 21 4 43 39 83.03 1.15 22 4 44 40 84.00 0.67 23 5 13 8 84.96 0.65 24 5 16 11 84.30 0.70 25 5 20 15 84.64 0.72 26 5 27 22 85.39 0.53 27 5 28 23 85.45 0.53 28 5 29 24 85.56 0.48 29 5 30 25 85.61 0.50 30 5 44 39 84.03 0.67 31 5 46 41 84.10 0.68 32 6 30 24 85.61 0.50 33 6 33 27 85.11 0.34 34 6 37 31 85.55 0.47 35 6 43 37 84.34 0.72 36 6 44 38 84.28 0.73 37 6 45 39 84.16 0.68 38 6 46 40 84.14 0.69 39 6 55 49 84.39 0.65 40 7 10 3 870.12 2.54 41 7 13 6 206.09 51.39 42 7 30 23 85.97 0.54 43 7 31 24 85.62 0.53 44 7 46 39 84.27 0.69 45 8 46 38 84.70 0.69 46 10 13 3 838.03 3.35 47 12 15 3 845.57 1.34 48 12 16 4 824.89 3.00 49 12 81 69 88.42 1.02 50 13 16 3 844.88 1.64 51 13 17 4 821.75 0.21 52 13 29 16 96.78 1.98 53 14 17 3 839.95 2.00 54 14 18 4 824.35 1.51 55 15 18 3 835.45 0.26 56 15 19 4 824.35 1.51 57 15 84 69 94.52 3.06 58 16 19 3 855.21 5.63 59 16 20 4 824.37 1.53 60 16 44 28 95.23 2.74 61 16 46 30 94.58 3.06 62 16 68 52 94.56 3.07 63 16 84 68 94.19 3.23 64 17 20 3 837.37 2.35 65 17 21 4 821.67 2.88 66 17 27 10 97.46 1.86 67 17 29 12 97.37 1.90 68 18 21 3 822.25 2.96 69 18 22 4 825.64 1.27 70 19 22 3 843.98 0.91 71 19 23 4 824.79 1.69 72 19 84 65 94.66 3.10 73 19 85 66 94.65 3.10 74 19 88 69 94.66 3.10 75 20 23 3 828.33 2.85 76 20 24 4 821.66 2.90 77 20 25 5 768.12 4.12 78 20 27 7 205.10 51.79 79 20 42 22 96.78 2.12 80 20 44 24 96.69 2.17 81 20 88 68 96.38 2.25 82 21 24 3 833.60 2.87 83 23 88 65 96.70 2.16 84 23 89 66 96.68 2.15 85 23 92 69 96.71 2.17 86 23 93 70 96.70 2.17 87 25 42 17 96.96 2.04 88 28 40 12 102.33 3.31 89 30 34 4 868.85 0.97 90 30 36 6 774.18 6.86 91 30 37 7 334.75 8.21 92 30 40 10 210.86 48.63 93 33 37 4 844.83 8.38 94 33 54 21 105.84 1.38 95 33 55 22 104.57 2.02 96 33 58 25 108.99 2.16 97 34 37 3 868.69 2.73 98 34 58 24 109.03 2.18 99 35 38 3 872.18 3.58 100 35 58 23 111.20 1.13 101 36 39 3 869.65 2.25 102 36 40 4 869.51 2.31 103 37 40 3 869.36 2.39 104 37 43 6 260.29 58.65 105 37 55 18 160.56 11.36 106 37 59 22 167.32 8.16 107 37 63 26 167.50 8.14 108 38 59 21 167.34 8.15 109 38 60 22 178.42 2.66 110 38 61 23 167.40 8.18 111 38 63 25 167.39 8.18 112 40 63 23 202.19 0.35 113 41 63 22 202.28 0.38 114 41 64 23 202.28 0.38 115 41 95 54 202.29 0.39 116 42 63 21 202.29 0.38 117 42 64 22 202.28 0.38 118 42 92 50 202.34 0.41 119 42 95 53 202.29 0.39 120 42 232 190 202.27 0.38 121 43 55 12 203.53 0.09 122 43 59 16 203.47 0.11 123 43 63 20 202.69 0.26 124 43 64 21 202.53 0.34 125 43 65 22 202.38 0.42 126 43 66 23 202.53 0.35 127 44 66 22 202.71 0.27 128 44 68 24 202.95 0.25 129 45 51 6 529.02 115.25 130 45 52 7 207.95 2.24 131 45 55 10 213.33 0.23 132 45 65 20 202.95 0.25 133 45 66 21 202.90 0.23 134 45 67 22 202.90 0.23 135 45 68 23 202.98 0.24 136 46 68 22 207.94 0.18 137 46 70 24 227.51 0.01 138 47 51 4 815.39 4.53 139 47 68 21 227.15 0.10 140 47 69 22 227.41 0.02 141 47 70 23 227.52 0.01 142 47 71 24 228.39 0.42 143 48 51 3 832.99 1.77 144 48 52 4 826.48 0.83 145 48 69 21 227.52 0.01 146 48 71 23 228.40 0.42 147 49 52 3 842.70 5.61 148 49 53 4 826.51 0.82 149 49 74 25 229.16 0.35 150 49 75 26 229.02 0.28 151 49 76 27 228.35 0.42 152 49 77 28 227.55 0.01 153 50 53 3 854.94 0.51 154 50 54 4 826.57 0.80 155 51 54 3 859.30 2.69 156 51 55 4 826.59 0.78 157 52 55 3 860.33 3.25 158 52 56 4 824.38 2.52 159 52 65 13 232.43 0.70 160 52 67 15 232.43 0.71 161 52 76 24 232.50 0.67 162 52 77 25 232.38 0.70 163 52 231 179 232.41 0.71 164 53 56 3 851.71 7.55 165 53 57 4 833.94 7.44 166 53 76 23 234.69 0.15 167 53 114 61 237.50 0.38 168 53 115 62 237.62 0.34 169 53 209 156 245.31 0.32 170 54 57 3 855.20 5.80 171 54 58 4 845.56 10.64 172 54 211 157 261.42 0.68 173 55 58 3 862.33 2.26 174 55 59 4 841.55 3.64 175 55 65 10 244.47 0.28 176 56 59 3 853.31 2.25 177 56 62 6 793.99 0.46 178 56 65 9 326.00 40.48 179 56 102 46 256.19 1.28 180 56 114 58 255.26 0.82 181 56 229 173 250.52 1.42 182 56 231 175 245.82 0.40 183 57 111 54 280.12 0.49 184 57 112 55 272.45 0.94 185 57 209 152 278.95 0.10 186 57 211 154 280.17 0.30 187 57 212 155 280.25 0.31 188 59 62 3 855.72 1.03 189 59 63 4 861.32 1.01 190 59 65 6 822.58 5.29 191 59 102 43 280.66 0.54 192 59 112 53 280.74 0.55 193 60 102 42 280.72 0.56 194 60 112 52 280.78 0.55 195 60 227 167 280.45 0.65 196 61 227 166 280.69 0.57 197 62 65 3 1010.13 1.67 198 62 102 40 280.83 0.56 199 62 228 166 280.72 0.56 200 62 229 167 280.70 0.56 201 62 230 168 280.62 0.54 202 64 95 31 280.81 0.56 203 64 97 33 280.64 0.53 204 64 230 166 280.73 0.56 205 64 231 167 280.70 0.56 206 64 232 168 280.64 0.53 207 65 78 13 353.38 27.34 208 65 230 165 280.80 0.56 209 65 231 166 280.73 0.57 210 65 232 167 280.72 0.57 211 66 78 12 353.38 27.34 212 66 79 13 353.38 27.34 213 66 87 21 281.20 0.49 214 66 88 22 281.19 0.48 215 66 91 25 281.06 0.48 216 66 232 166 280.72 0.57 217 67 77 10 353.38 27.34 218 67 78 11 353.38 27.34 219 67 79 12 353.38 27.34 220 68 79 11 353.38 27.34 221 68 80 12 353.37 27.34 222 68 81 13 353.37 27.34 223 68 84 16 288.92 4.13 224 69 72 3 904.47 3.48 225 69 74 5 353.47 27.34 226 69 77 8 353.42 27.34 227 69 79 10 353.39 27.34 228 69 80 11 353.37 27.34 229 69 81 12 353.37 27.34 230 70 81 11 353.41 27.31 231 72 247 175 166.52 1.67 232 72 248 176 153.17 4.55 233 72 249 177 152.13 4.59 234 73 244 171 169.38 0.61 235 73 247 174 162.31 0.14 236 73 248 175 153.16 4.55 237 74 77 3 826.53 0.62 238 74 244 170 170.91 0.15 239 75 156 81 281.40 0.33 240 75 237 162 254.25 5.40 241 75 241 166 175.75 0.53 242 75 244 169 170.92 0.15 243 76 114 38 281.48 0.41 244 76 153 77 281.37 0.35 245 76 231 155 281.17 0.53 246 77 234 157 280.64 0.60 247 78 231 153 281.15 0.52 248 78 232 154 280.94 0.59 249 78 234 156 280.62 0.60 250 79 83 4 809.62 4.38 251 79 84 5 447.72 66.47 252 79 87 8 289.82 4.67 253 80 83 3 853.68 1.80 254 80 84 4 809.61 4.38 255 81 84 3 848.10 4.59 256 83 86 3 877.58 2.56 257 83 87 4 829.72 2.75 258 84 87 3 840.20 6.90 259 84 88 4 829.71 2.75 260 85 88 3 852.05 0.97 261 85 89 4 829.70 2.76 262 86 89 3 847.51 3.60 263 86 90 4 832.95 1.86 264 87 90 3 853.19 0.77 265 87 91 4 832.17 1.48 266 88 91 3 833.90 2.34 267 88 92 4 832.39 1.59 268 89 92 3 847.03 2.31 269 89 93 4 832.39 1.59 270 90 93 3 854.84 6.58 271 90 94 4 820.81 1.31 272 91 95 4 800.25 0.04 273 91 232 141 281.55 0.36 274 92 95 3 809.72 7.27 275 92 232 140 282.54 0.07 276 95 232 137 286.50 1.32 277 96 232 136 286.51 1.32 278 96 233 137 286.49 1.32 279 97 230 133 288.18 1.96 280 97 231 134 288.03 1.95 281 97 232 135 286.51 1.32 282 97 233 136 286.50 1.32 283 98 170 72 506.54 0.70 284 98 230 132 288.20 1.95 285 98 231 133 288.09 1.98 286 98 233 135 286.50 1.32 287 98 234 136 286.74 1.44 288 98 237 139 283.76 0.09 289 99 228 129 292.26 3.31 290 99 229 130 289.40 2.16 291 99 230 131 288.93 2.18 292 100 114 14 505.94 0.79 293 100 153 53 505.97 0.78 294 100 169 69 506.70 0.74 295 100 170 70 506.70 0.73 296 100 228 128 293.58 2.81 297 100 229 129 290.83 2.70 298 100 231 131 289.48 2.14 299 100 237 137 262.23 10.60 300 101 114 13 506.00 0.77 301 101 151 50 506.06 0.73 302 101 152 51 506.70 0.73 303 101 172 71 506.75 0.72 304 101 193 92 506.66 0.76 305 101 224 123 338.65 11.98 306 101 228 127 293.92 2.64 307 102 112 10 506.28 0.62 308 102 113 11 506.29 0.62 309 102 114 12 506.00 0.77 310 102 224 122 360.53 1.04 311 102 225 123 338.60 12.00 312 102 227 125 320.95 10.60 313 102 228 126 293.95 2.64 314 102 229 127 293.94 2.64 315 103 112 9 506.67 0.70 316 103 113 10 506.36 0.66 317 103 117 14 505.93 0.78 318 103 151 48 506.75 0.72 319 103 174 71 506.76 0.72 320 103 193 90 506.79 0.72 321 103 222 119 373.58 3.63 322 103 223 120 371.06 2.40 323 103 224 121 360.53 1.04 324 103 225 122 338.61 12.00 325 104 111 7 506.72 0.70 326 104 112 8 506.72 0.70 327 104 222 118 374.04 3.43 328 104 223 119 373.56 3.62 329 105 108 3 855.23 2.21 330 105 109 4 802.72 10.46 331 105 117 12 506.69 0.74 332 105 206 101 505.97 0.75 333 105 220 115 378.70 4.97 334 105 221 116 378.17 4.88 335 105 222 117 373.81 3.74 336 106 109 3 824.99 0.67 337 106 220 114 378.88 5.00 338 106 221 115 378.36 4.83 339 107 206 99 506.56 0.78 340 107 214 107 506.10 0.69 341 107 216 109 503.94 0.04 342 107 220 113 378.84 5.02 343 108 111 3 839.27 4.61 344 108 206 98 506.66 0.76 345 108 207 99 506.75 0.73 346 108 208 100 506.77 0.73 347 108 212 104 506.74 0.73 348 108 213 105 506.64 0.77 349 108 214 106 506.31 0.74 350 111 208 97 506.82 0.72 351 111 212 101 506.55 0.85 352 112 225 113 364.96 3.26 353 112 227 115 337.44 12.10 354 113 116 3 846.77 2.85 355 113 117 4 805.44 10.43 356 113 208 95 506.96 0.68 357 113 209 96 514.00 0.62 358 114 151 37 581.88 3.14 359 114 153 39 581.95 3.14 360 114 209 95 544.23 1.23 361 114 229 115 293.96 2.66 362 114 231 117 293.98 2.67 363 115 150 35 581.93 3.12 364 115 151 36 581.93 3.12 365 115 153 38 581.96 3.14 366 115 154 39 581.95 3.14 367 115 209 94 544.40 1.18 368 116 148 32 582.45 3.19 369 116 149 33 582.08 3.04 370 116 150 34 582.08 3.04 371 116 158 42 582.64 3.20 372 116 208 92 544.79 1.25 373 116 209 93 544.71 1.24 374 116 210 94 544.71 1.24 375 117 148 31 582.55 3.16 376 117 149 32 582.45 3.19 377 117 206 89 544.89 1.26 378 117 207 90 544.85 1.27 379 117 208 91 544.80 1.25 380 117 222 105 373.59 3.63 381 118 147 29 582.60 3.15 382 118 148 30 582.55 3.16 383 118 158 40 582.85 3.11 384 118 206 88 544.90 1.26 385 118 207 89 544.85 1.27 386 119 128 9 583.81 3.11 387 119 146 27 583.07 3.12 388 119 147 28 582.83 3.12 389 119 199 80 577.35 3.66 390 119 201 82 570.23 0.79 391 119 204 85 545.07 1.24 392 119 205 86 545.02 1.22 393 119 206 87 544.90 1.26 394 119 216 97 543.67 1.26 395 120 128 8 583.81 3.11 396 120 145 25 583.63 3.17 397 120 146 26 583.21 3.20 398 120 158 38 583.45 3.12 399 120 179 59 583.60 3.17 400 120 204 84 545.09 1.24 401 120 205 85 545.07 1.24 402 120 207 87 545.01 1.23 403 121 126 5 583.89 3.10 404 121 128 7 583.81 3.11 405 121 145 24 583.76 3.10 406 122 203 81 567.53 0.46 407 122 204 82 560.27 2.08 408 122 205 83 545.69 1.15 409 122 215 93 544.72 1.27 410 122 216 94 544.71 1.27 411 122 217 95 544.70 1.26 412 123 126 3 844.89 3.20 413 127 143 16 622.29 8.34 414 127 144 17 621.73 8.29 415 127 182 55 622.20 8.48 416 128 143 15 622.49 8.43 417 128 144 16 622.18 8.48 418 128 146 18 622.23 8.53 419 128 201 73 576.92 3.85 420 128 202 74 576.71 3.94 421 128 203 75 570.86 1.09 422 128 204 76 570.00 1.21 423 129 141 12 623.07 8.40 424 129 142 13 622.82 8.50 425 129 143 14 622.62 8.46 426 129 201 72 577.35 3.67 427 130 141 11 623.07 8.40 428 130 142 12 623.01 8.40 429 130 144 14 622.83 8.46 430 130 147 17 622.50 8.44 431 130 199 69 580.32 3.71 432 130 200 70 579.91 3.77 433 130 201 71 577.40 3.64 434 131 140 9 623.16 8.38 435 131 141 10 623.08 8.40 436 131 199 68 580.36 3.70 437 131 200 69 580.18 3.64 438 132 138 6 623.47 8.36 439 132 139 7 623.23 8.39 440 132 140 8 623.18 8.38 441 132 147 15 622.89 8.44 442 132 176 44 622.64 8.45 443 132 189 57 622.66 8.44 444 132 197 65 582.24 3.14 445 132 198 66 581.34 3.45 446 132 199 67 581.01 3.58 447 133 136 3 844.82 2.95 448 133 137 4 748.12 1.68 449 133 138 5 623.48 8.36 450 133 197 64 582.31 3.10 451 133 198 65 581.36 3.44 452 133 200 67 581.34 3.45 453 134 137 3 835.06 2.16 454 134 139 5 748.13 1.70 455 134 176 42 622.82 8.46 456 134 196 62 582.82 3.06 457 134 197 63 582.33 3.10 458 135 195 60 583.01 3.08 459 135 196 61 582.89 3.07 460 136 198 62 582.78 3.08 461 139 189 50 622.65 8.46 462 140 183 43 736.28 0.38 463 140 189 49 622.65 8.45 464 141 183 42 736.33 0.39 465 142 147 5 811.85 2.41 466 142 178 36 707.52 0.84 467 142 181 39 736.38 0.42 468 142 183 41 736.31 0.39 469 142 186 44 724.78 1.01 470 143 181 38 736.40 0.42 471 143 182 39 736.35 0.39 472 144 178 34 736.33 0.40 473 144 179 35 736.82 0.23 474 144 180 36 736.82 0.29 475 144 181 37 736.40 0.42 476 145 179 34 737.01 0.33 477 145 180 35 736.91 0.29 478 146 178 32 737.37 0.45 479 146 179 33 737.03 0.33 480 147 176 29 737.81 0.32 481 147 177 30 737.69 0.33 482 147 178 31 737.38 0.45 483 147 179 32 737.37 0.46 484 148 158 10 737.99 0.40 485 148 159 11 737.98 0.40 486 148 175 27 738.04 0.38 487 148 176 28 737.81 0.31 488 148 177 29 737.75 0.31 489 148 179 31 737.51 0.41 490 149 174 25 738.25 0.46 491 149 175 26 738.09 0.39 492 149 176 27 737.99 0.38 493 149 197 48 580.34 3.54 494 149 199 50 576.79 3.81 495 149 222 73 374.01 3.51 496 150 154 4 793.26 11.30 497 150 159 9 738.11 0.40 498 150 174 24 738.89 0.13 499 150 175 25 738.22 0.45 500 151 154 3 834.29 2.50 501 151 155 4 834.10 2.59 502 151 173 22 740.45 0.36 503 151 175 24 739.02 0.09 504 151 193 42 736.04 0.56 505 152 155 3 834.09 2.58 506 152 167 15 741.82 0.52 507 152 168 16 741.88 0.54 508 152 169 17 741.88 0.54 509 152 171 19 741.77 0.52 510 152 172 20 741.32 0.52 511 152 173 21 741.06 0.58 512 152 175 23 740.12 0.21 513 152 193 41 736.81 0.34 514 153 156 3 876.80 0.54 515 153 169 16 741.90 0.54 516 154 157 3 833.89 0.09 517 154 159 5 773.34 1.75 518 155 159 4 799.57 11.37 519 155 160 5 799.57 11.37 520 155 165 10 742.48 0.53 521 155 166 11 742.27 0.45 522 155 167 12 742.08 0.51 523 155 175 20 741.39 0.47 524 156 160 4 804.52 8.89 525 156 166 10 742.31 0.47 526 156 241 85 190.02 0.99 527 156 245 89 170.99 0.13 528 156 248 92 163.81 0.61 529 157 160 3 835.98 0.83 530 157 161 4 827.26 2.36 531 158 161 3 846.00 4.17 532 158 162 4 827.24 2.35 533 158 179 21 740.91 0.64 534 159 162 3 839.06 3.54 535 159 163 4 827.25 2.35 536 159 164 5 763.38 3.37 537 159 175 16 741.86 0.57 538 159 177 18 741.69 0.54 539 160 163 3 843.21 5.94 540 160 164 4 769.71 0.10 541 160 166 6 753.34 0.35 542 160 245 85 172.38 0.16 543 160 248 88 166.38 1.34 544 162 177 15 741.83 0.58 545 162 178 16 741.53 0.70 546 162 179 17 740.99 0.54 547 162 186 24 737.04 0.34 548 163 177 14 741.83 0.58 549 164 177 13 741.79 0.57 550 164 188 24 736.95 0.35 551 165 175 10 741.96 0.56 552 165 188 23 737.01 0.35 553 165 190 25 736.95 0.35 554 166 241 75 197.24 1.21 555 166 245 79 173.68 0.51 556 167 171 4 825.99 5.00 557 167 173 6 742.57 0.38 558 167 175 8 741.96 0.55 559 168 171 3 844.20 3.66 560 168 238 70 225.46 6.99 561 168 241 73 188.33 0.52 562 169 237 68 226.56 6.95 563 169 241 72 179.02 0.67 564 170 237 67 250.07 4.02 565 172 193 21 736.88 0.34 566 172 194 22 736.88 0.34 567 172 224 52 370.03 2.23 568 173 190 17 736.95 0.34 569 173 191 18 736.89 0.34 570 173 193 20 736.64 0.26 571 174 190 16 736.91 0.35 572 174 191 17 736.87 0.33 573 174 192 18 736.82 0.32 574 174 193 19 735.10 0.14 575 174 195 21 717.38 0.95 576 174 197 23 591.77 8.67 577 174 222 48 374.65 3.31 578 174 223 49 374.00 3.42 579 175 189 14 736.92 0.35 580 175 190 15 736.88 0.35 581 176 188 12 736.91 0.35 582 176 189 13 736.89 0.35 583 176 191 15 736.82 0.32 584 176 197 21 581.02 3.58 585 177 186 9 737.01 0.34 586 177 188 11 736.89 0.35 587 178 181 3 850.62 1.13 588 178 186 8 736.99 0.37 589 181 186 5 748.19 5.24 590 183 186 3 896.72 5.42 591 191 195 4 833.07 0.09 592 191 197 6 753.18 5.59 593 192 195 3 862.28 0.60 594 193 224 31 370.09 2.02 595 194 224 30 371.00 2.38 596 196 221 25 378.66 4.95 597 196 222 26 378.12 4.76 598 196 223 27 374.69 3.26 599 197 221 24 378.62 4.96 600 197 222 25 377.88 4.83 601 198 219 21 425.60 7.42 602 198 220 22 378.97 4.95 603 198 221 23 378.66 4.95 604 199 218 19 533.36 1.86 605 199 219 20 445.79 1.84 606 199 220 21 379.01 4.95 607 199 222 23 378.85 5.01 608 200 218 18 533.25 1.91 609 200 219 19 458.94 0.93 610 201 204 3 860.80 5.92 611 201 216 15 544.07 1.13 612 201 217 16 543.25 1.38 613 201 218 17 539.52 1.22 614 202 217 15 543.67 1.26 615 202 218 16 543.02 1.50 616 203 217 14 543.75 1.27 617 204 215 11 544.45 1.20 618 204 216 12 544.24 1.17 619 205 213 8 544.74 1.24 620 205 214 9 544.46 1.20 621 205 215 10 544.25 1.20 622 205 216 11 543.77 1.22 623 206 213 7 544.45 1.20 624 206 214 8 544.41 1.19 625 206 216 10 543.55 1.28 626 206 220 14 401.38 6.23 627 207 210 3 841.45 0.14 628 207 211 4 835.56 2.77 629 207 212 5 648.82 50.83 630 207 213 6 544.09 1.30 631 208 211 3 855.23 1.75 632 208 212 4 753.97 1.22 633 216 220 4 867.44 0.94 634 231 237 6 792.96 2.00 635 234 237 3 840.72 2.90 636 236 239 3 839.14 1.80 637 236 240 4 815.12 5.39 638 237 240 3 837.00 2.87 639 237 241 4 825.14 0.39 640 238 241 3 839.35 4.31 641 238 242 4 841.27 4.97 642 240 243 3 848.90 0.47 643 240 244 4 815.02 5.10 644 241 244 3 847.27 4.61 645 241 245 4 813.04 5.55 646 242 245 3 843.95 2.94 647 242 246 4 813.06 2.43 648 243 246 3 855.08 7.53 649 243 247 4 826.00 6.87 650 244 247 3 840.26 0.26 651 244 248 4 818.24 2.99 652 245 248 3 842.82 1.54