BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 41 40 660.54 54.72 2 1 42 41 660.55 54.73 3 1 95 94 66.25 0.06 4 2 25 23 674.14 49.86 5 2 26 24 674.11 49.86 6 2 28 26 674.10 49.86 7 2 39 37 660.70 54.73 8 2 40 38 660.60 54.73 9 2 41 39 660.57 54.73 10 2 42 40 660.56 54.73 11 3 20 17 674.25 49.89 12 3 25 22 674.21 49.88 13 3 26 23 674.21 49.88 14 3 27 24 674.11 49.86 15 3 28 25 674.11 49.86 16 3 40 37 660.66 54.74 17 3 42 39 660.57 54.73 18 4 28 24 674.12 49.86 19 4 30 26 674.09 49.85 20 4 37 33 660.73 54.73 21 4 40 36 660.71 54.73 22 4 42 38 660.65 54.75 23 4 43 39 660.57 54.73 24 4 44 40 660.55 54.73 25 5 13 8 674.55 49.84 26 5 16 11 674.44 49.89 27 5 20 15 674.41 49.88 28 5 27 22 674.24 49.88 29 5 28 23 674.20 49.88 30 5 29 24 674.10 49.86 31 5 30 25 674.10 49.86 32 5 44 39 660.57 54.73 33 5 46 41 660.54 54.73 34 6 30 24 674.10 49.86 35 6 33 27 662.29 54.59 36 6 37 31 660.88 54.75 37 6 43 37 660.69 54.75 38 6 44 38 660.65 54.75 39 6 45 39 660.57 54.73 40 6 46 40 660.55 54.73 41 6 51 45 567.59 54.73 42 6 55 49 370.05 43.99 43 7 10 3 870.12 0.69 44 7 13 6 674.78 49.85 45 7 29 22 674.23 49.87 46 7 30 23 674.16 49.86 47 7 31 24 674.08 49.84 48 7 46 39 660.56 54.73 49 8 46 38 660.64 54.75 50 8 47 39 660.60 54.75 51 8 48 40 660.61 54.76 52 8 51 43 585.79 52.02 53 8 70 62 367.12 43.99 54 9 13 4 862.59 0.24 55 10 13 3 864.10 0.99 56 12 15 3 862.60 0.24 57 12 16 4 843.55 2.64 58 12 46 34 660.64 54.74 59 12 70 58 367.11 43.98 60 12 81 69 219.55 4.20 61 13 16 3 850.40 0.79 62 13 17 4 844.23 2.30 63 13 29 16 674.30 49.91 64 14 17 3 844.23 2.30 65 14 18 4 839.58 0.03 66 15 18 3 850.41 0.80 67 15 19 4 839.87 0.12 68 15 84 69 149.60 5.59 69 16 19 3 843.02 0.50 70 16 20 4 839.86 0.12 71 16 44 28 660.64 54.74 72 16 46 30 660.53 54.73 73 16 68 52 367.47 44.13 74 16 84 68 130.60 3.58 75 17 20 3 850.16 5.47 76 17 21 4 834.47 2.81 77 17 27 10 674.34 49.91 78 17 29 12 674.33 49.91 79 18 21 3 839.66 0.23 80 18 22 4 839.66 0.20 81 19 22 3 842.77 0.73 82 19 23 4 838.68 1.31 83 19 84 65 129.68 4.04 84 19 85 66 126.50 2.58 85 19 88 69 125.17 2.25 86 20 23 3 838.68 1.31 87 20 24 4 844.88 4.47 88 20 25 5 787.20 5.14 89 20 27 7 674.53 49.91 90 20 42 22 660.70 54.73 91 20 44 24 660.56 54.74 92 20 88 68 124.13 1.77 93 21 24 3 846.62 3.60 94 21 25 4 830.87 4.20 95 22 85 63 129.53 4.09 96 23 88 65 124.17 1.76 97 23 89 66 124.09 1.79 98 23 92 69 105.14 5.64 99 25 42 17 660.62 54.73 100 28 40 12 660.71 54.73 101 30 36 6 761.75 6.88 102 30 37 7 660.87 54.75 103 30 40 10 660.79 54.73 104 33 37 4 851.57 0.80 105 33 54 21 370.10 43.98 106 33 55 22 370.05 43.99 107 33 58 25 369.89 44.06 108 34 37 3 867.72 0.26 109 34 58 24 369.50 43.89 110 35 38 3 867.72 0.26 111 35 58 23 369.43 43.91 112 36 39 3 864.49 1.86 113 36 40 4 863.49 2.37 114 37 40 3 863.21 1.23 115 37 43 6 660.70 54.77 116 37 55 18 369.99 43.97 117 37 59 22 368.94 43.91 118 37 63 26 368.20 44.09 119 38 59 21 368.88 43.88 120 38 60 22 368.88 43.88 121 38 61 23 368.31 44.09 122 38 63 25 368.20 44.09 123 40 63 23 368.14 44.11 124 41 63 22 368.13 44.11 125 41 64 23 368.13 44.11 126 41 95 54 96.65 2.02 127 42 63 21 368.12 44.11 128 42 64 22 367.97 44.04 129 42 88 46 124.10 1.79 130 42 92 50 105.78 4.71 131 42 95 53 96.91 2.02 132 42 232 190 87.98 0.81 133 43 55 12 369.94 43.94 134 43 59 16 368.94 43.91 135 43 63 20 368.20 44.09 136 43 64 21 368.14 44.11 137 43 65 22 367.93 44.06 138 43 66 23 367.92 44.05 139 44 66 22 367.69 44.17 140 44 68 24 367.47 44.13 141 44 88 44 124.10 1.79 142 45 51 6 771.28 5.92 143 45 52 7 370.03 43.98 144 45 55 10 370.03 43.98 145 45 65 20 368.17 44.09 146 45 66 21 367.94 44.06 147 45 67 22 367.60 44.19 148 45 68 23 367.60 44.19 149 46 68 22 367.47 44.13 150 46 70 24 367.11 43.98 151 47 51 4 825.98 5.32 152 47 68 21 367.69 44.17 153 47 69 22 367.45 44.12 154 47 70 23 367.08 43.98 155 47 71 24 366.91 44.02 156 48 51 3 863.84 4.99 157 48 52 4 837.45 0.51 158 48 69 21 367.66 44.16 159 48 71 23 366.68 44.04 160 49 52 3 840.75 1.14 161 49 53 4 836.56 0.07 162 49 74 25 230.37 3.02 163 49 75 26 226.88 1.28 164 49 76 27 222.05 3.68 165 50 53 3 837.79 0.78 166 50 54 4 836.45 0.11 167 51 54 3 841.15 1.28 168 51 55 4 836.45 0.11 169 52 55 3 841.36 2.56 170 52 56 4 841.35 2.56 171 52 65 13 368.43 44.06 172 52 67 15 368.13 44.12 173 52 76 24 222.02 3.68 174 52 77 25 221.97 3.66 175 52 231 179 88.69 0.52 176 53 56 3 841.32 2.58 177 53 57 4 837.35 1.16 178 53 76 23 222.05 3.69 179 53 114 61 99.02 2.71 180 53 115 62 100.78 2.44 181 53 209 156 101.13 2.29 182 53 211 158 101.73 2.42 183 54 57 3 843.07 1.70 184 54 58 4 837.36 1.16 185 54 211 157 101.42 2.54 186 55 58 3 853.09 6.71 187 55 59 4 837.36 1.16 188 55 65 10 368.44 44.05 189 56 59 3 842.44 1.38 190 56 62 6 795.41 0.26 191 56 65 9 368.53 44.02 192 56 102 46 97.28 1.92 193 56 114 58 98.91 2.75 194 56 229 173 90.33 0.92 195 56 231 175 88.72 0.52 196 57 111 54 97.91 1.67 197 57 112 55 97.34 1.92 198 57 208 151 101.35 2.53 199 57 209 152 101.39 2.55 200 57 211 154 101.62 2.46 201 57 212 155 101.64 2.44 202 59 62 3 846.76 0.90 203 59 63 4 862.06 2.21 204 59 65 6 833.49 1.09 205 59 102 43 97.20 1.90 206 60 102 42 97.14 1.86 207 60 112 52 97.31 1.90 208 60 227 167 91.52 0.41 209 61 227 166 91.50 0.40 210 62 65 3 1002.38 1.25 211 62 228 166 90.97 0.60 212 62 229 167 88.67 0.53 213 62 230 168 88.32 0.70 214 64 95 31 97.21 1.89 215 64 97 33 91.20 0.36 216 64 230 166 88.14 0.78 217 64 231 167 88.00 0.81 218 64 232 168 87.86 0.89 219 65 78 13 221.84 3.63 220 65 230 165 88.31 0.71 221 65 231 166 88.02 0.81 222 65 232 167 87.98 0.81 223 66 78 12 221.82 3.64 224 66 79 13 221.81 3.63 225 66 87 21 124.10 1.79 226 66 88 22 122.98 2.35 227 66 91 25 112.18 7.45 228 66 232 166 87.98 0.81 229 67 77 10 221.84 3.64 230 67 78 11 221.83 3.64 231 67 79 12 221.83 3.64 232 68 79 11 221.79 3.62 233 68 80 12 221.64 3.58 234 68 81 13 221.20 3.39 235 68 84 16 130.01 4.09 236 69 73 4 845.33 0.32 237 69 74 5 222.08 3.67 238 69 77 8 221.92 3.63 239 69 79 10 221.84 3.64 240 69 80 11 221.72 3.62 241 69 81 12 221.69 3.61 242 70 81 11 221.66 3.57 243 73 244 171 32.77 0.61 244 73 247 174 23.62 0.04 245 73 248 175 18.96 0.62 246 74 77 3 822.69 4.51 247 74 244 170 35.06 0.70 248 75 156 81 100.83 2.03 249 75 241 166 41.88 0.81 250 75 244 169 33.73 0.14 251 75 248 173 22.50 0.19 252 76 114 38 101.50 2.40 253 76 153 77 100.59 2.13 254 76 231 155 90.52 1.02 255 77 234 157 87.77 0.89 256 78 231 153 88.81 0.56 257 78 232 154 88.67 0.53 258 78 234 156 84.05 1.65 259 79 83 4 836.65 0.20 260 79 84 5 184.33 15.26 261 79 87 8 126.53 2.56 262 80 83 3 840.45 0.18 263 80 84 4 790.29 7.48 264 81 84 3 848.45 1.00 265 84 87 3 842.06 2.44 266 84 88 4 827.12 2.00 267 85 88 3 841.40 2.77 268 85 89 4 839.02 3.95 269 86 89 3 849.81 0.10 270 86 90 4 827.13 2.00 271 87 90 3 844.27 2.68 272 87 91 4 827.10 2.00 273 88 91 3 842.79 3.14 274 88 92 4 827.10 2.00 275 89 92 3 849.72 1.81 276 89 93 4 823.08 3.10 277 90 93 3 832.81 1.52 278 90 94 4 821.94 3.67 279 91 95 4 803.08 5.76 280 91 232 141 87.87 0.81 281 92 95 3 834.70 0.93 282 95 232 137 88.66 0.53 283 96 232 136 88.67 0.53 284 96 233 137 88.41 0.65 285 97 230 133 100.98 1.29 286 97 231 134 95.39 1.46 287 97 232 135 88.69 0.53 288 97 233 136 88.67 0.53 289 98 170 72 319.98 20.40 290 98 230 132 102.29 0.66 291 98 231 133 95.41 1.45 292 98 233 135 88.69 0.53 293 98 234 136 88.69 0.53 294 98 237 139 84.56 1.40 295 99 228 129 105.59 1.46 296 99 229 130 105.48 1.47 297 99 230 131 104.47 1.73 298 100 114 14 319.52 20.37 299 100 152 52 319.87 20.43 300 100 153 53 319.74 20.38 301 100 169 69 320.07 20.39 302 100 170 70 320.19 20.40 303 100 228 128 106.53 1.93 304 100 229 129 105.55 1.45 305 100 231 131 105.55 1.45 306 100 237 137 88.04 0.73 307 101 114 13 319.74 20.37 308 101 152 51 320.09 20.39 309 101 172 71 320.16 20.39 310 101 193 92 320.11 20.39 311 101 224 123 158.96 6.16 312 101 228 127 106.63 1.98 313 102 112 10 319.83 20.41 314 102 113 11 319.73 20.46 315 102 114 12 319.74 20.37 316 102 224 122 160.78 7.04 317 102 225 123 148.43 0.91 318 102 227 125 116.56 2.58 319 102 228 126 107.01 1.83 320 102 229 127 107.01 1.83 321 103 112 9 319.89 20.43 322 103 113 10 319.88 20.43 323 103 117 14 319.63 20.43 324 103 151 48 320.17 20.39 325 103 174 71 320.18 20.39 326 103 222 119 170.07 5.92 327 103 223 120 169.75 6.00 328 103 224 121 162.54 6.17 329 103 225 122 158.94 6.16 330 104 111 7 319.89 20.43 331 104 112 8 319.91 20.43 332 104 222 118 170.08 5.92 333 104 223 119 169.99 5.91 334 104 225 121 162.19 6.34 335 105 108 3 839.52 2.26 336 105 109 4 807.25 4.65 337 105 117 12 320.06 20.39 338 105 206 101 319.63 20.43 339 105 220 115 196.03 1.15 340 105 221 116 195.23 1.12 341 105 222 117 175.91 3.00 342 106 109 3 834.11 0.61 343 106 220 114 196.05 1.15 344 106 221 115 195.88 1.12 345 107 206 99 319.94 20.41 346 107 214 107 319.88 20.41 347 107 216 109 319.42 20.37 348 107 220 113 196.08 1.16 349 108 111 3 832.03 0.54 350 108 206 98 320.12 20.42 351 108 207 99 320.19 20.39 352 108 208 100 320.24 20.40 353 108 212 104 320.11 20.40 354 108 213 105 320.04 20.41 355 108 214 106 319.93 20.41 356 111 208 97 320.33 20.41 357 111 212 101 320.30 20.41 358 112 225 113 160.75 7.05 359 112 227 115 148.37 0.92 360 113 116 3 849.05 7.53 361 113 117 4 810.90 8.15 362 113 208 95 320.50 20.34 363 113 209 96 337.75 20.89 364 114 151 37 570.11 63.09 365 114 153 39 570.13 63.09 366 114 209 95 357.66 20.39 367 114 229 115 106.67 2.00 368 114 231 117 106.67 2.00 369 115 150 35 570.14 63.05 370 115 151 36 570.15 63.09 371 115 153 38 570.14 63.09 372 115 154 39 570.10 63.07 373 115 209 94 357.68 20.39 374 116 148 32 570.28 62.97 375 116 149 33 570.20 63.04 376 116 150 34 570.14 63.07 377 116 158 42 570.37 62.95 378 116 208 92 357.83 20.42 379 116 209 93 357.76 20.38 380 117 148 31 570.39 62.97 381 117 149 32 570.30 63.02 382 117 206 89 358.00 20.45 383 117 207 90 357.99 20.44 384 117 208 91 357.84 20.42 385 117 220 103 196.05 1.15 386 117 222 105 170.08 5.92 387 118 147 29 570.43 62.95 388 118 148 30 570.39 62.97 389 118 158 40 570.59 62.88 390 118 206 88 358.01 20.44 391 118 207 89 357.98 20.45 392 119 128 9 571.26 62.55 393 119 146 27 571.00 62.66 394 119 147 28 570.57 62.88 395 119 199 80 399.47 19.54 396 119 201 82 398.19 19.07 397 119 204 85 358.58 20.34 398 119 205 86 358.22 20.52 399 119 206 87 358.01 20.44 400 119 216 97 357.58 20.45 401 119 220 101 239.55 22.84 402 120 128 8 571.27 62.55 403 120 145 25 571.22 62.55 404 120 146 26 571.01 62.66 405 120 158 38 571.29 62.60 406 120 179 59 571.21 62.55 407 120 204 84 361.81 18.75 408 120 205 85 358.22 20.51 409 120 207 87 358.02 20.44 410 121 126 5 571.29 62.54 411 121 128 7 571.27 62.55 412 121 145 24 571.26 62.55 413 121 203 82 392.48 19.20 414 121 204 83 374.31 20.37 415 122 203 81 396.05 19.38 416 122 204 82 388.92 19.73 417 122 205 83 379.62 17.73 418 122 215 93 357.80 20.38 419 122 216 94 357.79 20.38 420 122 217 95 357.79 20.38 421 123 126 3 852.72 10.04 422 123 203 80 397.92 19.42 423 127 143 16 602.37 47.21 424 127 144 17 601.86 47.31 425 127 145 18 601.85 47.30 426 127 182 55 602.19 47.13 427 128 143 15 602.45 47.19 428 128 144 16 602.37 47.19 429 128 146 18 602.31 47.22 430 128 201 73 399.35 19.53 431 128 202 74 398.66 19.29 432 128 203 75 398.39 19.30 433 128 204 76 392.45 19.18 434 129 141 12 602.82 47.23 435 129 142 13 602.67 47.22 436 129 143 14 602.50 47.16 437 129 201 72 399.38 19.52 438 130 141 11 602.83 47.24 439 130 142 12 602.68 47.22 440 130 144 14 602.59 47.16 441 130 147 17 602.49 47.28 442 130 199 69 403.07 19.95 443 130 200 70 401.50 20.45 444 130 201 71 399.37 19.52 445 131 140 9 602.89 47.23 446 131 141 10 602.83 47.24 447 131 199 68 403.17 19.90 448 131 200 69 401.77 20.57 449 132 138 6 603.39 47.23 450 132 139 7 603.25 47.26 451 132 140 8 603.03 47.29 452 132 147 15 602.78 47.23 453 132 176 44 602.70 47.30 454 132 189 57 441.43 33.39 455 132 197 65 408.49 17.68 456 132 198 66 408.29 17.72 457 132 199 67 407.26 17.86 458 133 136 3 854.51 0.88 459 133 137 4 654.40 22.58 460 133 138 5 603.39 47.23 461 133 139 6 603.39 47.23 462 133 197 64 408.51 17.68 463 133 198 65 408.41 17.67 464 133 200 67 408.29 17.72 465 134 137 3 831.87 0.10 466 134 139 5 671.31 31.03 467 134 176 42 603.02 47.36 468 134 191 57 441.41 33.40 469 134 195 61 409.61 17.88 470 134 196 62 409.54 17.91 471 134 197 63 408.50 17.68 472 135 195 60 431.89 29.00 473 135 196 61 409.60 17.89 474 140 183 43 645.08 25.88 475 140 189 49 441.49 33.37 476 141 183 42 645.03 25.84 477 142 147 5 735.13 37.39 478 142 178 36 635.31 31.71 479 142 181 39 645.12 25.80 480 142 183 41 645.00 25.83 481 142 186 44 597.66 9.86 482 143 181 38 645.12 25.80 483 143 182 39 645.06 25.80 484 144 178 34 646.10 26.36 485 144 179 35 646.25 26.27 486 144 180 36 645.96 26.14 487 144 181 37 645.12 25.80 488 145 179 34 646.36 26.22 489 145 180 35 646.26 26.24 490 146 178 32 646.88 26.30 491 146 179 33 646.40 26.20 492 147 176 29 648.82 27.00 493 147 177 30 648.12 26.84 494 147 178 31 646.88 26.30 495 147 179 32 646.88 26.30 496 148 158 10 654.68 29.50 497 148 159 11 654.65 29.51 498 148 162 14 654.67 29.52 499 148 175 27 649.77 27.05 500 148 176 28 648.86 27.00 501 148 177 29 648.69 26.98 502 148 179 31 648.11 26.84 503 149 174 25 654.91 28.73 504 149 175 26 649.83 27.04 505 149 176 27 649.60 27.09 506 149 197 48 402.97 19.98 507 149 199 50 399.22 19.43 508 149 222 73 170.08 5.92 509 150 154 4 716.74 32.58 510 150 159 9 654.73 29.50 511 150 174 24 654.97 28.70 512 150 175 25 652.32 27.75 513 151 154 3 825.17 0.24 514 151 155 4 822.00 1.34 515 151 173 22 655.94 28.45 516 151 175 24 654.98 28.71 517 151 193 42 483.32 54.97 518 152 155 3 842.35 5.04 519 152 167 15 657.54 28.10 520 152 168 16 657.49 28.07 521 152 169 17 657.26 28.01 522 152 171 19 657.23 28.03 523 152 172 20 657.13 28.03 524 152 173 21 657.05 28.04 525 152 175 23 656.00 28.41 526 152 193 41 483.44 55.02 527 153 156 3 897.20 2.94 528 153 169 16 657.31 27.99 529 154 157 3 825.85 2.63 530 154 159 5 792.90 5.83 531 155 159 4 792.90 5.83 532 155 160 5 792.90 5.83 533 155 165 10 657.89 28.02 534 155 166 11 657.58 28.09 535 155 167 12 657.56 28.09 536 155 175 20 656.98 28.04 537 156 160 4 805.29 6.67 538 156 166 10 657.60 28.09 539 156 241 85 50.43 0.93 540 156 245 89 38.12 0.07 541 156 248 92 29.61 0.67 542 157 160 3 824.75 3.06 543 157 161 4 822.60 1.99 544 158 161 3 834.20 0.41 545 158 162 4 822.60 1.99 546 158 179 21 656.56 28.17 547 159 162 3 822.61 2.00 548 159 163 4 822.60 1.99 549 159 164 5 714.50 34.02 550 159 175 16 657.12 28.02 551 159 177 18 656.81 28.11 552 160 163 3 825.64 0.47 553 160 164 4 718.52 36.05 554 160 166 6 676.26 34.45 555 160 245 85 40.86 0.29 556 160 248 88 35.02 1.62 557 162 177 15 657.07 28.05 558 162 178 16 656.96 28.04 559 162 179 17 656.53 28.19 560 162 186 24 484.35 54.73 561 163 177 14 657.07 28.05 562 164 177 13 657.04 28.05 563 164 188 24 484.11 54.75 564 165 175 10 657.15 28.02 565 165 188 23 484.16 54.77 566 165 190 25 484.07 54.77 567 166 241 75 70.73 3.35 568 166 245 79 41.36 0.22 569 167 171 4 823.75 2.31 570 167 173 6 658.75 28.44 571 167 175 8 657.36 27.89 572 168 171 3 853.05 1.66 573 168 238 70 87.41 0.77 574 168 241 73 54.85 0.77 575 169 237 68 87.76 0.71 576 169 241 72 52.57 0.36 577 170 237 67 88.66 0.52 578 170 238 68 86.55 0.42 579 172 193 21 483.60 54.95 580 172 224 52 166.33 4.30 581 173 190 17 483.86 54.88 582 173 191 18 483.74 54.90 583 173 193 20 483.32 54.97 584 174 190 16 483.80 54.88 585 174 191 17 483.74 54.89 586 174 192 18 483.67 54.91 587 174 193 19 482.43 54.51 588 174 195 21 475.80 51.20 589 174 197 23 409.54 18.37 590 174 222 48 183.65 0.86 591 174 223 49 170.00 5.88 592 175 189 14 484.04 54.77 593 175 190 15 483.76 54.87 594 176 188 12 484.07 54.75 595 176 189 13 484.00 54.75 596 176 191 15 483.76 54.86 597 176 197 21 407.54 17.98 598 177 186 9 484.26 54.73 599 177 188 11 484.04 54.73 600 178 181 3 857.21 3.37 601 178 186 8 484.25 54.71 602 181 186 5 663.62 29.07 603 183 186 3 892.08 5.19 604 191 195 4 835.76 1.28 605 191 197 6 696.57 14.23 606 192 195 3 844.19 2.93 607 193 224 31 168.93 6.03 608 194 224 30 169.88 5.93 609 196 221 25 195.62 0.99 610 197 221 24 195.58 0.96 611 197 222 25 194.60 0.72 612 198 219 21 253.14 16.28 613 198 220 22 196.18 1.11 614 198 221 23 195.72 0.98 615 199 219 20 298.93 4.67 616 199 220 21 239.76 22.90 617 199 222 23 196.16 1.12 618 200 218 18 347.62 18.64 619 200 219 19 298.93 4.67 620 201 204 3 876.31 5.88 621 201 216 15 357.64 20.46 622 201 217 16 357.39 20.55 623 201 218 17 355.69 20.66 624 202 217 15 357.42 20.55 625 202 218 16 357.39 20.55 626 203 217 14 357.72 20.41 627 204 215 11 357.79 20.39 628 204 216 12 357.74 20.40 629 205 214 9 357.79 20.39 630 205 215 10 357.70 20.41 631 205 216 11 357.48 20.50 632 206 213 7 357.79 20.39 633 206 214 8 357.74 20.41 634 206 216 10 357.39 20.55 635 206 220 14 239.49 22.87 636 207 210 3 858.03 0.59 637 207 211 4 828.14 0.45 638 207 212 5 666.66 30.43 639 207 213 6 357.69 20.41 640 208 211 3 857.85 0.68 641 208 212 4 789.47 7.59 642 216 220 4 846.49 0.53 643 231 237 6 736.94 25.55 644 234 237 3 836.48 0.80 645 236 239 3 821.41 1.82 646 236 240 4 807.33 3.83 647 237 240 3 842.75 2.32 648 237 241 4 817.96 0.08 649 238 241 3 848.66 5.30 650 238 242 4 854.09 2.59 651 240 243 3 863.92 5.29 652 240 244 4 820.39 2.56 653 241 244 3 836.91 1.01 654 241 245 4 820.40 2.58 655 242 245 3 860.30 0.06 656 242 246 4 820.39 2.58 657 243 246 3 839.25 4.57 658 243 247 4 818.06 1.66 659 244 247 3 827.27 1.94 660 244 248 4 817.99 1.63 661 245 248 3 830.11 2.19