BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 497 95 98 514.45 0.88 2 497 96 99 520.44 2.11 3 498 95 97 550.73 1.15 4 498 96 98 552.75 1.78 5 499 96 97 570.42 2.09 6 500 92 92 570.64 1.99 7 500 95 95 570.93 1.95 8 500 96 96 570.97 1.93 9 500 241 241 31.57 0.34 10 1 96 95 571.02 1.91 11 1 97 96 570.98 1.92 12 1 98 97 570.97 1.93 13 1 237 236 38.81 0.78 14 1 241 240 31.59 0.33 15 2 92 90 571.02 1.91 16 2 98 96 570.98 1.92 17 2 100 98 570.95 1.93 18 2 122 120 460.44 2.91 19 2 123 121 460.41 2.92 20 2 125 123 459.06 3.22 21 2 126 124 459.06 3.22 22 2 241 239 32.91 0.54 23 3 97 94 571.09 1.91 24 3 98 95 571.02 1.91 25 3 99 96 570.99 1.92 26 3 100 97 570.98 1.92 27 3 126 123 459.06 3.22 28 3 128 125 459.02 3.23 29 3 237 234 42.88 0.37 30 4 100 96 570.98 1.92 31 4 102 98 570.93 1.92 32 4 109 105 476.03 2.48 33 4 119 115 461.05 2.77 34 4 125 121 460.30 2.91 35 4 126 122 459.19 3.20 36 4 127 123 459.05 3.21 37 4 128 124 459.03 3.23 38 5 13 8 571.01 1.93 39 5 16 11 571.09 1.92 40 5 17 12 571.07 1.93 41 5 20 15 571.31 1.82 42 5 99 94 571.11 1.92 43 5 100 95 571.01 1.91 44 5 102 97 570.97 1.93 45 5 128 123 459.06 3.23 46 5 233 228 57.33 1.50 47 6 13 7 571.09 1.92 48 6 102 96 570.99 1.92 49 6 106 100 499.90 0.10 50 6 109 103 476.19 2.41 51 6 127 121 460.27 2.91 52 6 128 122 459.21 3.19 53 6 129 123 459.05 3.21 54 6 130 124 459.04 3.22 55 6 152 146 338.76 0.65 56 6 156 150 338.42 0.68 57 7 11 4 815.11 6.40 58 7 13 6 572.60 2.68 59 7 106 99 525.85 0.23 60 7 129 122 459.18 3.20 61 7 130 123 459.06 3.22 62 8 11 3 858.28 1.14 63 8 104 96 570.99 1.92 64 8 106 98 550.90 0.75 65 9 104 95 570.98 1.92 66 9 105 96 570.98 1.92 67 10 166 156 336.91 0.83 68 11 130 119 459.10 3.25 69 11 165 154 335.16 0.55 70 11 166 155 335.15 0.55 71 12 15 3 851.42 0.98 72 12 16 4 827.62 2.98 73 12 165 153 335.13 0.55 74 12 166 154 333.47 0.13 75 13 16 3 836.43 3.96 76 13 17 4 833.13 5.61 77 13 101 88 572.09 1.70 78 13 102 89 571.94 1.62 79 13 103 90 571.85 1.66 80 13 104 91 571.06 1.86 81 13 130 117 458.98 3.24 82 14 17 3 836.09 4.71 83 14 18 4 838.86 3.32 84 14 42 28 572.55 1.42 85 14 43 29 572.77 1.38 86 14 44 30 573.25 1.18 87 14 101 87 572.23 1.65 88 15 18 3 860.03 0.47 89 15 19 4 844.28 6.03 90 15 43 28 572.76 1.38 91 15 224 209 92.80 2.64 92 16 19 3 856.13 0.11 93 16 20 4 838.32 3.05 94 16 130 114 458.95 3.25 95 16 165 149 326.75 2.19 96 16 222 206 116.53 1.41 97 16 224 208 84.53 1.50 98 16 230 214 84.03 1.46 99 17 20 3 858.48 1.28 100 17 21 4 840.67 1.87 101 17 32 15 573.43 1.37 102 17 44 27 573.45 1.23 103 17 99 82 573.09 1.52 104 17 101 84 572.37 1.61 105 18 21 3 851.74 1.02 106 18 22 4 847.33 1.19 107 18 32 14 573.51 1.30 108 18 44 26 573.38 1.24 109 19 22 3 847.33 1.19 110 19 23 4 847.33 1.19 111 19 224 205 84.52 1.50 112 19 227 208 84.42 1.53 113 19 230 211 83.67 1.50 114 20 23 3 848.26 0.27 115 20 24 4 846.88 0.96 116 20 99 79 573.59 1.30 117 20 230 210 66.21 4.07 118 20 233 213 59.20 0.57 119 20 234 214 59.25 0.66 120 21 24 3 854.63 1.15 121 21 25 4 834.60 5.18 122 21 30 9 574.01 1.45 123 21 32 11 573.65 1.28 124 21 99 78 573.65 1.32 125 22 25 3 850.99 2.49 126 22 32 10 574.05 1.45 127 22 227 205 84.42 1.53 128 23 26 3 884.41 6.62 129 23 227 204 84.41 1.52 130 23 230 207 76.23 0.94 131 23 231 208 66.23 4.06 132 23 234 211 59.30 0.63 133 24 27 3 875.49 4.05 134 24 28 4 809.21 1.56 135 24 30 6 574.74 1.13 136 24 97 73 574.46 1.26 137 24 99 75 574.24 1.31 138 24 234 210 58.84 0.64 139 25 28 3 861.94 3.71 140 25 30 5 721.01 6.21 141 25 32 7 721.01 6.21 142 28 96 68 682.86 3.31 143 28 97 69 682.84 3.31 144 28 234 206 56.91 1.60 145 29 94 65 682.98 3.31 146 29 95 66 682.93 3.31 147 29 96 67 682.88 3.31 148 29 97 68 682.88 3.31 149 30 95 65 682.98 3.31 150 30 97 67 682.88 3.31 151 30 99 69 682.86 3.31 152 31 47 16 805.19 1.94 153 31 91 60 682.99 3.31 154 31 95 64 683.04 3.29 155 31 97 66 682.98 3.31 156 31 98 67 682.88 3.31 157 31 99 68 682.88 3.31 158 32 44 12 805.23 1.92 159 32 45 13 805.23 1.92 160 32 46 14 805.20 1.94 161 32 47 15 805.20 1.94 162 32 48 16 805.23 1.95 163 32 99 67 682.89 3.31 164 33 48 15 805.23 1.96 165 33 88 55 683.37 3.25 166 33 91 58 683.34 3.24 167 33 98 65 683.04 3.29 168 33 99 66 682.97 3.32 169 33 100 67 682.88 3.31 170 33 101 68 682.88 3.31 171 34 39 5 824.72 0.06 172 34 42 8 805.52 1.95 173 34 44 10 805.50 1.96 174 34 48 14 805.30 1.97 175 34 49 15 805.24 1.97 176 34 50 16 805.25 1.97 177 34 51 17 805.25 1.97 178 34 101 67 682.90 3.32 179 35 50 15 805.27 1.97 180 35 74 39 695.78 4.59 181 35 100 65 683.05 3.29 182 35 101 66 682.96 3.33 183 35 102 67 682.89 3.31 184 35 103 68 682.88 3.32 185 35 109 74 476.14 2.42 186 35 114 79 468.10 1.05 187 35 118 83 461.28 2.83 188 36 39 3 852.85 1.38 189 36 50 14 805.40 1.95 190 36 51 15 805.40 1.96 191 36 74 38 695.89 4.61 192 36 102 66 682.96 3.33 193 36 103 67 682.88 3.32 194 36 104 68 682.88 3.32 195 37 50 13 805.46 1.98 196 37 51 14 805.40 1.96 197 37 54 17 804.16 1.75 198 37 55 18 771.04 11.00 199 37 56 19 771.03 11.00 200 37 73 36 695.91 4.55 201 37 74 37 695.87 4.58 202 37 77 40 695.84 4.58 203 38 56 18 771.03 11.01 204 39 42 3 959.08 3.51 205 39 51 12 805.47 1.98 206 40 51 11 805.49 1.97 207 42 46 4 859.99 2.35 208 42 49 7 805.52 1.97 209 42 51 9 805.49 1.97 210 44 101 57 682.90 3.33 211 46 49 3 864.35 3.46 212 47 91 44 683.05 3.31 213 48 84 36 683.45 3.26 214 48 87 39 683.39 3.25 215 48 88 40 683.38 3.25 216 48 91 43 683.35 3.24 217 49 52 3 858.09 1.77 218 50 53 3 858.57 1.53 219 50 54 4 817.41 1.67 220 50 77 27 693.97 4.74 221 50 84 34 683.50 3.27 222 51 54 3 833.01 9.38 223 51 55 4 807.83 3.04 224 52 84 32 683.59 3.31 225 53 76 23 695.94 4.55 226 53 77 24 695.88 4.57 227 53 80 27 690.28 6.38 228 53 84 31 683.65 3.28 229 54 58 4 851.99 1.62 230 54 59 5 842.88 2.93 231 54 73 19 695.94 4.55 232 54 76 22 695.94 4.55 233 54 77 23 695.89 4.58 234 55 58 3 857.87 0.56 235 55 59 4 858.44 1.60 236 56 59 3 871.46 4.91 237 56 60 4 851.98 1.61 238 56 69 13 696.07 4.57 239 56 70 14 696.06 4.55 240 56 73 17 696.01 4.58 241 57 60 3 861.88 3.25 242 57 61 4 858.45 1.61 243 58 61 3 861.82 3.29 244 59 62 3 852.82 3.08 245 59 64 5 727.52 10.92 246 59 69 10 696.15 4.55 247 59 72 13 696.12 4.56 248 59 76 17 696.10 4.56 249 60 63 3 843.71 1.12 250 60 64 4 727.53 10.91 251 60 65 5 727.53 10.90 252 60 66 6 719.41 6.84 253 60 69 9 696.21 4.58 254 62 76 14 696.40 4.64 255 64 68 4 813.69 1.07 256 64 69 5 777.53 17.00 257 64 72 8 754.83 5.66 258 64 76 12 747.89 9.13 259 65 68 3 836.59 4.66 260 65 69 4 826.55 0.21 261 66 69 3 821.71 2.87 262 66 70 4 826.56 0.21 263 67 70 3 861.48 1.23 264 67 71 4 834.70 1.58 265 68 71 3 856.86 3.54 266 68 72 4 829.32 1.12 267 69 72 3 855.30 2.56 268 69 73 4 829.38 1.15 269 70 73 3 858.53 0.94 270 70 74 4 855.30 2.55 271 70 105 35 682.98 3.33 272 70 108 38 679.51 2.33 273 71 74 3 869.88 1.22 274 71 75 4 848.60 0.81 275 72 75 3 855.37 4.20 276 72 76 4 850.53 1.09 277 73 76 3 855.48 1.61 278 73 77 4 852.16 1.91 279 74 77 3 853.53 2.58 280 74 78 4 856.07 3.85 281 74 103 29 683.08 3.29 282 74 108 34 654.84 5.23 283 74 112 38 535.50 27.77 284 74 114 40 474.03 1.64 285 75 78 3 862.99 0.41 286 75 79 4 856.08 3.85 287 75 112 37 535.54 27.79 288 76 79 3 859.47 2.87 289 77 80 3 859.26 5.45 290 77 81 4 840.78 3.36 291 77 84 7 767.56 18.84 292 77 114 37 468.73 0.98 293 78 81 3 837.04 5.23 294 78 112 34 535.51 27.78 295 78 113 35 473.87 1.58 296 78 114 36 468.63 0.94 297 78 117 39 468.49 0.99 298 80 83 3 847.34 0.22 299 80 84 4 844.64 4.08 300 81 84 3 853.14 0.08 301 81 85 4 843.81 4.51 302 81 114 33 468.58 0.93 303 81 117 36 467.58 1.19 304 81 118 37 465.68 0.68 305 82 85 3 845.49 3.75 306 82 86 4 843.81 4.51 307 83 86 3 850.62 1.10 308 83 87 4 843.80 4.51 309 84 87 3 843.90 4.46 310 84 88 4 840.81 3.04 311 84 114 30 468.54 0.91 312 85 88 3 845.24 5.21 313 85 89 4 840.78 3.05 314 85 118 33 461.26 2.83 315 85 121 36 460.51 2.89 316 85 122 37 460.44 2.91 317 86 89 3 855.21 0.23 318 86 90 4 840.61 3.14 319 87 90 3 863.36 2.43 320 87 91 4 850.82 1.97 321 88 91 3 863.36 2.43 322 88 92 4 851.62 1.56 323 88 98 10 683.11 3.29 324 88 100 12 683.08 3.30 325 88 118 30 461.22 2.81 326 88 122 34 460.44 2.91 327 89 92 3 864.30 2.90 328 89 93 4 841.31 3.59 329 89 122 33 460.44 2.92 330 89 123 34 460.43 2.92 331 90 93 3 842.90 3.72 332 90 94 4 841.29 3.60 333 91 94 3 844.90 1.79 334 91 95 4 834.84 0.38 335 91 98 7 683.09 3.30 336 92 95 3 841.44 2.99 337 92 98 6 683.08 3.32 338 92 123 31 460.26 2.90 339 95 98 3 892.09 1.35 340 97 234 137 56.06 1.67 341 97 237 140 38.81 0.78 342 100 122 22 460.52 2.91 343 102 109 7 476.13 2.42 344 103 106 3 860.49 1.53 345 103 108 5 714.98 13.88 346 103 109 6 476.18 2.41 347 105 108 3 853.38 5.08 348 105 109 4 838.38 2.40 349 106 109 3 838.39 2.40 350 106 110 4 824.13 0.09 351 106 129 23 460.32 2.92 352 106 148 42 339.11 0.67 353 106 152 46 338.93 0.69 354 107 110 3 824.13 0.09 355 107 111 4 814.47 4.91 356 107 112 5 814.48 4.91 357 108 112 4 766.50 19.08 358 109 115 6 462.52 2.31 359 109 116 7 466.43 0.59 360 109 119 10 463.75 1.70 361 109 156 47 338.57 0.70 362 110 116 6 468.06 0.91 363 110 148 38 339.11 0.67 364 110 152 42 338.89 0.70 365 110 155 45 338.58 0.72 366 110 156 46 338.57 0.70 367 111 155 44 338.69 0.76 368 112 115 3 879.77 0.99 369 112 116 4 847.47 2.07 370 113 116 3 866.69 7.54 371 113 117 4 837.68 2.10 372 114 117 3 872.69 4.54 373 114 118 4 837.69 2.10 374 115 118 3 860.91 0.27 375 115 119 4 845.83 2.30 376 116 119 3 855.08 2.61 377 116 120 4 849.19 0.62 378 116 155 39 338.35 0.64 379 116 156 40 338.07 0.77 380 117 120 3 854.12 3.08 381 117 121 4 846.33 0.81 382 118 121 3 862.79 5.22 383 118 122 4 845.02 1.44 384 119 122 3 861.66 0.96 385 119 125 6 616.21 74.95 386 119 127 8 460.44 2.92 387 119 156 37 337.73 0.70 388 119 158 39 337.65 0.74 389 122 125 3 893.25 5.76 390 124 240 116 36.34 0.70 391 124 241 117 34.17 0.09 392 124 244 120 28.93 1.66 393 125 158 33 337.56 0.70 394 125 241 116 34.35 0.01 395 126 159 33 337.56 0.70 396 126 161 35 337.36 0.71 397 126 233 107 57.33 1.50 398 126 237 111 45.15 0.21 399 126 240 114 37.29 1.10 400 127 152 25 338.34 0.45 401 127 156 29 337.97 0.71 402 127 158 31 337.69 0.73 403 127 159 32 337.59 0.71 404 127 160 33 337.41 0.74 405 127 161 34 337.35 0.72 406 128 161 33 337.42 0.74 407 128 163 35 337.28 0.72 408 128 220 92 125.49 0.36 409 128 222 94 123.95 0.34 410 128 233 105 57.33 1.50 411 129 145 16 339.03 0.68 412 129 148 19 338.92 0.67 413 129 149 20 338.82 0.69 414 129 152 23 338.74 0.64 415 129 160 31 337.64 0.74 416 129 161 32 337.55 0.72 417 129 162 33 337.35 0.74 418 129 163 34 337.31 0.72 419 130 163 33 337.05 0.85 420 130 165 35 335.26 0.61 421 130 222 92 125.31 0.45 422 131 135 4 854.07 3.18 423 131 145 14 338.98 0.66 424 131 163 32 337.38 0.76 425 131 164 33 337.04 0.85 426 131 165 34 336.99 0.86 427 132 135 3 863.59 2.21 428 133 136 3 859.08 3.10 429 133 165 32 337.41 0.78 430 133 166 33 337.38 0.77 431 133 170 37 307.38 0.20 432 134 137 3 902.05 4.53 433 135 141 6 749.24 5.99 434 135 145 10 526.38 92.59 435 136 141 5 782.94 22.83 436 136 142 6 746.15 4.44 437 136 145 9 596.25 57.65 438 136 164 28 337.60 0.80 439 136 178 42 214.94 0.54 440 136 182 46 184.36 5.29 441 137 141 4 840.85 0.52 442 138 141 3 862.36 0.57 443 138 142 4 856.96 3.27 444 139 142 3 869.81 3.15 445 139 143 4 856.97 3.27 446 139 181 42 184.35 5.31 447 139 191 52 152.25 0.34 448 139 192 53 151.06 0.28 449 140 143 3 858.38 2.56 450 140 144 4 853.24 1.41 451 141 144 3 849.00 3.53 452 141 145 4 840.58 0.33 453 142 145 3 855.91 1.31 454 142 146 4 838.62 1.26 455 142 178 36 188.25 3.37 456 142 181 39 176.09 1.94 457 142 182 40 176.21 1.99 458 143 146 3 835.20 2.97 459 143 147 4 835.19 2.98 460 143 207 64 126.23 0.36 461 144 147 3 842.54 3.98 462 144 148 4 834.09 0.69 463 145 148 3 855.71 2.77 464 145 149 4 838.94 0.79 465 146 149 3 865.20 3.44 466 146 150 4 843.27 2.96 467 146 182 36 175.41 2.25 468 146 217 71 125.83 0.32 469 146 219 73 125.72 0.32 470 147 150 3 853.81 2.26 471 147 151 4 843.26 2.96 472 147 206 59 126.26 0.37 473 148 151 3 849.85 0.34 474 148 152 4 843.25 2.96 475 149 152 3 853.25 1.36 476 149 153 4 849.85 0.34 477 149 160 11 338.06 0.80 478 149 162 13 337.69 0.76 479 150 153 3 849.84 0.34 480 150 154 4 849.83 0.35 481 151 154 3 857.75 0.94 482 151 155 4 839.97 2.02 483 152 155 3 857.29 4.33 484 152 156 4 838.38 1.24 485 152 160 8 337.99 0.80 486 153 156 3 861.40 2.27 487 153 157 4 786.43 14.29 488 153 158 5 548.86 104.50 489 153 159 6 339.83 0.03 490 153 160 7 338.04 0.82 491 154 157 3 836.65 0.36 492 159 240 81 38.85 0.80 493 161 217 56 125.70 0.33 494 161 218 57 125.67 0.33 495 161 220 59 125.52 0.35 496 161 236 75 55.05 1.19 497 162 217 55 125.73 0.32 498 162 219 57 125.61 0.36 499 162 220 58 125.54 0.34 500 163 219 56 125.70 0.33 501 163 220 57 125.53 0.34 502 163 222 59 125.51 0.35 503 164 167 3 902.74 1.57 504 164 178 14 176.26 2.04 505 164 182 18 174.92 2.02 506 164 220 56 125.71 0.33 507 164 221 57 125.53 0.35 508 164 222 58 125.51 0.35 509 165 222 57 125.28 0.43 510 166 169 3 869.90 5.08 511 166 222 56 125.53 0.35 512 167 170 3 852.97 1.86 513 167 175 8 215.40 0.33 514 167 221 54 125.71 0.33 515 167 222 55 125.65 0.34 516 167 223 56 125.52 0.35 517 167 228 61 91.54 1.95 518 168 223 55 125.64 0.35 519 168 225 57 125.48 0.35 520 168 226 58 124.96 0.31 521 170 174 4 741.46 3.50 522 170 175 5 575.41 86.52 523 170 178 8 550.71 74.17 524 171 174 3 846.16 4.12 525 172 175 3 831.34 0.26 526 172 176 4 829.13 1.35 527 173 176 3 838.58 1.19 528 173 177 4 838.34 1.35 529 174 177 3 842.81 0.89 530 174 178 4 839.71 1.94 531 175 178 3 855.40 2.40 532 175 179 4 840.86 1.38 533 176 179 3 857.43 4.21 534 176 180 4 841.18 1.54 535 177 180 3 865.60 0.11 536 177 181 4 865.60 0.12 537 178 181 3 869.51 1.29 538 178 182 4 854.70 5.56 539 179 182 3 861.88 2.52 540 179 183 4 834.06 2.02 541 179 221 42 125.87 0.30 542 180 184 4 769.66 2.92 543 180 187 7 222.35 3.25 544 181 188 7 169.04 2.73 545 181 191 10 176.28 6.35 546 182 219 37 125.89 0.30 547 182 221 39 125.89 0.30 548 183 221 38 125.97 0.33 549 184 187 3 875.35 1.54 550 184 188 4 846.53 2.26 551 185 188 3 902.32 3.40 552 186 189 3 853.56 0.77 553 187 190 3 835.48 0.15 554 187 191 4 837.15 2.81 555 188 191 3 865.52 3.43 556 188 192 4 852.95 2.85 557 189 192 3 852.98 2.87 558 189 193 4 865.51 3.42 559 189 204 15 126.50 0.40 560 189 207 18 126.43 0.42 561 190 193 3 868.65 4.96 562 190 194 4 868.65 4.97 563 191 194 3 886.82 1.13 564 192 204 12 126.53 0.40 565 192 207 15 126.70 0.29 566 195 201 6 228.18 2.05 567 197 200 3 574.95 105.84 568 197 201 4 829.13 5.01 569 198 201 3 829.72 4.75 570 198 202 4 829.16 5.03 571 199 202 3 844.86 2.81 572 199 203 4 837.47 0.93 573 200 203 3 844.90 2.79 574 200 204 4 844.84 2.75 575 201 204 3 844.91 2.79 576 201 205 4 845.35 2.50 577 202 205 3 857.26 1.31 578 202 206 4 855.12 2.38 579 203 206 3 858.43 0.73 580 203 207 4 863.88 2.00 581 204 207 3 863.08 2.40 582 205 208 3 899.61 4.04 583 209 214 5 227.31 10.44 584 209 215 6 179.30 2.06 585 209 216 7 179.20 2.08 586 210 214 4 362.24 10.31 587 210 216 6 189.66 3.14 588 220 229 9 197.71 3.54 589 220 232 12 192.47 1.69 590 220 233 13 191.08 0.98 591 220 236 16 190.02 1.37 592 221 228 7 198.03 3.60 593 221 229 8 197.94 3.60 594 222 228 6 206.35 0.57 595 222 229 7 197.97 3.58 596 222 230 8 197.97 3.58 597 222 233 11 197.95 3.59 598 223 226 3 846.92 0.19 599 223 227 4 232.80 5.33 600 223 228 5 214.81 3.66 601 223 230 7 208.71 0.60 602 224 230 6 238.40 2.50 603 227 230 3 858.49 0.76 604 227 231 4 864.23 2.12 605 229 232 3 852.00 0.14 606 229 233 4 851.99 0.14 607 230 233 3 853.83 0.79 608 230 234 4 852.13 0.06 609 231 234 3 847.48 2.27 610 231 235 4 838.21 6.27 611 232 235 3 859.16 3.56 612 232 236 4 847.62 2.04 613 233 236 3 867.02 0.36 614 233 237 4 851.42 3.93 615 234 237 3 851.44 3.94 616 234 238 4 851.43 3.94 617 235 238 3 860.57 3.59 618 235 239 4 849.95 4.67 619 236 239 3 849.95 4.67 620 236 240 4 850.19 0.69 621 237 240 3 850.21 0.70 622 237 241 4 850.19 0.69 623 238 241 3 855.09 1.35 624 238 242 4 849.92 0.56 625 239 242 3 854.62 1.58 626 239 243 4 845.89 2.57 627 240 243 3 857.25 4.23 628 240 244 4 777.32 25.96