BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 203 200 174.06 0.05 2 3 204 201 173.65 0.23 3 3 205 202 169.33 0.01 4 3 224 221 116.14 0.30 5 3 227 224 115.71 0.12 6 4 36 32 395.79 27.21 7 4 37 33 395.80 27.20 8 4 205 201 171.99 1.06 9 5 36 31 395.79 27.21 10 5 37 32 395.82 27.20 11 5 227 222 116.22 0.24 12 5 228 223 116.20 0.24 13 5 229 224 116.22 0.24 14 5 243 238 42.62 0.66 15 5 247 242 22.43 0.35 16 6 37 31 395.82 27.20 17 6 39 33 396.24 26.99 18 6 90 84 346.56 2.66 19 6 124 118 332.32 2.93 20 6 161 155 332.25 2.92 21 6 205 199 182.44 0.26 22 6 207 201 171.55 0.94 23 6 227 221 116.31 0.24 24 6 228 222 116.23 0.23 25 6 229 223 116.22 0.24 26 7 36 29 396.32 27.02 27 7 37 30 396.31 27.03 28 7 38 31 396.25 26.98 29 7 39 32 396.25 26.98 30 7 229 222 116.23 0.23 31 7 231 224 116.20 0.24 32 7 240 233 52.09 0.72 33 7 243 236 42.62 0.66 34 7 244 237 41.01 0.21 35 8 39 31 396.25 26.98 36 8 229 221 116.31 0.25 37 8 230 222 116.21 0.24 38 8 231 223 116.20 0.24 39 9 39 30 396.36 27.02 40 9 40 31 396.34 26.98 41 9 41 32 396.55 26.94 42 9 231 222 116.23 0.23 43 9 235 226 77.70 0.12 44 9 240 231 54.13 0.24 45 10 41 31 397.03 26.73 46 10 64 54 396.35 26.84 47 10 95 85 347.57 2.16 48 10 230 220 116.82 0.23 49 10 231 221 116.29 0.24 50 10 232 222 116.20 0.24 51 10 235 225 84.89 1.41 52 11 14 3 854.82 0.30 53 11 20 9 398.78 26.35 54 11 23 12 398.74 26.38 55 11 40 29 397.23 26.67 56 11 41 30 397.25 26.68 57 11 42 31 397.28 26.64 58 11 43 32 397.35 26.64 59 11 64 53 397.11 26.55 60 11 235 224 107.00 0.88 61 11 236 225 84.46 1.42 62 12 43 31 397.37 26.63 63 12 64 52 397.12 26.55 64 12 95 83 348.55 2.27 65 12 97 85 348.56 2.27 66 12 232 220 116.80 0.24 67 13 43 30 397.75 26.45 68 14 43 29 397.78 26.44 69 14 236 222 115.70 0.48 70 15 19 4 825.55 4.77 71 15 20 5 824.07 5.19 72 15 46 31 400.14 26.05 73 16 19 3 846.29 5.79 74 16 20 4 836.11 0.43 75 16 46 30 400.49 25.94 76 17 20 3 855.93 0.97 77 17 21 4 839.72 1.36 78 17 46 29 400.50 25.95 79 17 49 32 401.49 25.48 80 18 21 3 855.59 1.14 81 18 22 4 843.41 3.21 82 19 22 3 849.95 3.73 83 19 23 4 839.72 1.37 84 20 23 3 847.12 5.12 85 20 24 4 839.66 1.39 86 20 42 22 402.24 25.17 87 20 43 23 402.28 25.18 88 20 46 26 402.31 25.19 89 20 50 30 402.31 25.19 90 21 24 3 859.20 0.41 91 21 25 4 847.49 5.42 92 21 49 28 402.45 25.18 93 21 53 32 402.49 25.20 94 22 25 3 847.63 5.36 95 22 26 4 838.73 0.91 96 23 26 3 866.25 0.21 97 23 27 4 847.66 5.37 98 23 235 212 97.75 1.42 99 23 236 213 82.56 0.99 100 24 27 3 847.86 5.45 101 24 28 4 846.76 4.92 102 24 40 16 402.39 25.23 103 24 53 29 404.14 25.01 104 24 54 30 403.07 25.09 105 25 28 3 869.60 1.89 106 25 29 4 844.27 3.66 107 25 55 30 405.10 24.64 108 26 29 3 865.29 3.35 109 26 30 4 838.71 0.94 110 26 237 211 78.69 0.61 111 26 238 212 74.44 1.02 112 26 241 215 64.03 0.78 113 27 30 3 843.31 3.29 114 27 40 13 402.67 25.29 115 27 240 213 65.16 0.22 116 27 244 217 52.62 1.01 117 28 53 25 413.54 20.41 118 28 54 26 405.09 24.64 119 28 55 27 405.10 24.64 120 28 56 28 405.12 24.63 121 30 56 26 515.63 3.96 122 30 241 211 53.67 1.56 123 31 245 214 45.22 0.63 124 32 36 4 861.16 2.56 125 32 38 6 691.22 57.98 126 32 56 24 538.12 0.52 127 32 58 26 530.38 3.36 128 32 59 27 530.37 3.35 129 32 244 212 45.22 0.62 130 33 36 3 886.94 3.60 131 33 58 25 544.62 0.12 132 33 244 211 45.22 0.62 133 33 245 212 40.99 0.23 134 34 58 24 550.51 3.06 135 36 58 22 574.16 0.42 136 36 244 208 40.86 0.29 137 37 58 21 574.20 0.40 138 37 90 53 406.53 26.82 139 37 205 168 182.56 0.26 140 38 58 20 574.22 0.40 141 38 59 21 574.20 0.41 142 38 60 22 574.20 0.41 143 38 244 206 38.91 0.54 144 39 60 21 574.20 0.41 145 39 61 22 574.18 0.42 146 39 62 23 574.15 0.42 147 39 90 51 406.67 27.20 148 39 124 85 393.39 24.18 149 40 54 14 574.33 0.39 150 40 59 19 574.31 0.38 151 40 60 20 574.24 0.42 152 40 61 21 574.19 0.42 153 40 62 22 574.19 0.42 154 40 240 200 56.48 1.41 155 41 62 21 574.19 0.42 156 41 63 22 574.18 0.42 157 41 64 23 574.11 0.43 158 41 92 51 408.99 27.98 159 42 46 4 808.53 2.10 160 42 47 5 736.83 27.93 161 42 50 8 574.72 0.32 162 42 61 19 574.32 0.38 163 42 62 20 574.29 0.38 164 42 64 22 574.17 0.42 165 43 46 3 859.16 0.11 166 43 47 4 819.90 3.58 167 43 64 21 574.20 0.41 168 44 47 3 844.69 6.17 169 44 78 34 410.69 27.79 170 46 49 3 844.75 7.12 171 46 50 4 838.73 10.12 172 47 50 3 852.47 3.25 173 47 51 4 852.20 3.39 174 47 61 14 574.38 0.38 175 47 62 15 574.36 0.38 176 47 63 16 574.33 0.38 177 47 83 36 409.85 27.88 178 47 86 39 409.81 27.90 179 47 88 41 409.31 27.98 180 48 51 3 853.30 2.85 181 48 52 4 846.39 0.48 182 48 86 38 409.76 27.91 183 49 52 3 854.12 0.80 184 49 53 4 849.23 1.65 185 50 53 3 854.12 0.80 186 50 54 4 849.23 1.65 187 50 61 11 574.38 0.38 188 51 54 3 859.93 0.54 189 51 61 10 574.40 0.36 190 51 86 35 409.81 27.90 191 51 87 36 409.43 27.82 192 51 88 37 409.16 27.96 193 54 59 5 574.61 0.30 194 54 61 7 574.59 0.31 195 56 59 3 864.84 3.00 196 60 89 29 409.09 27.93 197 60 90 30 409.03 27.96 198 61 88 27 409.26 28.00 199 61 89 28 409.12 27.93 200 61 90 29 409.03 27.96 201 62 88 26 409.36 28.05 202 62 90 28 409.02 27.96 203 63 83 20 409.88 27.88 204 63 90 27 409.26 28.01 205 63 91 28 409.11 27.94 206 63 92 29 409.11 27.94 207 64 92 28 409.12 27.97 208 65 78 13 410.70 27.79 209 65 91 26 409.75 27.87 210 65 92 27 409.41 28.00 211 65 93 28 409.29 28.03 212 65 98 33 409.60 27.91 213 65 112 47 410.16 27.78 214 66 78 12 410.69 27.78 215 66 80 14 410.64 27.80 216 66 83 17 410.57 27.75 217 66 91 25 409.78 27.86 218 66 93 27 409.76 27.86 219 66 112 46 410.23 27.79 220 67 74 7 411.56 27.62 221 67 77 10 410.93 27.76 222 67 78 11 410.82 27.81 223 67 108 41 410.27 27.80 224 67 112 45 410.27 27.81 225 68 108 40 410.36 27.77 226 68 111 43 410.37 27.77 227 68 112 44 410.28 27.80 228 68 115 47 410.43 27.75 229 69 74 5 785.33 4.18 230 69 115 46 410.57 27.78 231 70 79 9 410.88 27.79 232 70 81 11 410.85 27.78 233 70 115 45 410.82 27.80 234 71 74 3 954.25 2.10 235 74 77 3 872.37 0.51 236 79 82 3 858.01 7.07 237 79 83 4 839.68 3.54 238 80 83 3 840.33 3.22 239 80 84 4 839.68 3.54 240 80 91 11 413.38 27.05 241 80 115 35 414.04 26.94 242 80 116 36 413.77 27.04 243 80 119 39 461.28 3.68 244 80 121 41 414.07 26.89 245 81 84 3 854.15 2.18 246 81 85 4 839.68 3.54 247 81 119 38 463.48 2.73 248 82 85 3 847.38 0.09 249 82 86 4 837.09 4.83 250 83 86 3 862.61 2.18 251 83 87 4 834.51 3.53 252 83 88 5 623.26 18.72 253 83 91 8 521.98 26.58 254 84 87 3 830.63 2.46 255 84 88 4 803.48 3.61 256 84 119 35 622.83 1.63 257 84 121 37 593.75 4.16 258 89 121 32 653.77 0.67 259 89 122 33 653.75 0.67 260 90 122 32 653.75 0.67 261 90 123 33 653.75 0.67 262 90 124 34 653.75 0.67 263 90 161 71 653.75 0.67 264 91 116 25 653.97 0.65 265 91 121 30 653.90 0.65 266 91 122 31 653.86 0.66 267 91 123 32 653.78 0.68 268 91 124 33 653.75 0.67 269 92 95 3 916.22 1.14 270 92 124 32 653.78 0.68 271 92 126 34 653.77 0.68 272 93 109 16 653.94 0.66 273 93 112 19 654.08 0.65 274 93 113 20 653.99 0.66 275 93 116 23 654.13 0.64 276 93 123 30 653.93 0.64 277 93 124 31 653.89 0.66 278 93 125 32 653.79 0.68 279 93 126 33 653.79 0.68 280 94 99 5 783.64 2.48 281 94 109 15 653.98 0.66 282 94 126 32 653.80 0.68 283 95 98 3 847.13 3.11 284 95 99 4 827.83 0.26 285 95 126 31 653.79 0.68 286 95 165 70 653.72 0.65 287 95 230 135 117.56 0.48 288 96 99 3 832.84 0.62 289 96 100 4 822.70 2.30 290 96 165 69 653.70 0.64 291 96 167 71 649.88 0.96 292 97 100 3 851.57 1.12 293 97 101 4 794.96 3.12 294 97 102 5 794.96 3.12 295 98 102 4 794.93 3.13 296 98 103 5 794.93 3.13 297 98 104 6 655.41 0.58 298 99 103 4 727.75 36.73 299 99 104 5 655.34 0.60 300 99 106 7 655.40 0.58 301 99 109 10 655.32 0.61 302 99 126 27 654.07 0.65 303 99 127 28 654.03 0.66 304 99 146 47 654.16 0.67 305 100 103 3 848.28 1.07 306 104 108 4 837.80 4.83 307 104 109 5 834.15 1.33 308 104 112 8 813.56 7.38 309 105 108 3 853.44 2.98 310 105 109 4 842.23 2.62 311 106 109 3 855.60 3.66 312 106 110 4 842.23 2.62 313 106 145 39 654.36 0.64 314 106 146 40 654.29 0.66 315 106 149 43 654.38 0.64 316 107 110 3 842.23 2.62 317 107 111 4 842.23 2.62 318 107 153 46 655.70 1.02 319 108 111 3 854.60 1.78 320 108 112 4 834.92 1.03 321 109 112 3 845.35 4.96 322 109 113 4 832.25 0.42 323 109 125 16 654.37 0.65 324 110 113 3 856.18 1.14 325 110 114 4 837.28 2.94 326 110 149 39 654.59 0.58 327 110 150 40 654.56 0.59 328 110 153 43 655.65 1.00 329 111 114 3 846.96 5.76 330 111 115 4 841.35 4.98 331 112 115 3 862.82 1.32 332 112 116 4 841.34 4.99 333 113 116 3 855.67 1.43 334 113 117 4 839.84 4.25 335 113 123 10 654.84 0.68 336 113 125 12 654.75 0.70 337 113 154 41 666.19 3.44 338 113 160 47 656.60 1.35 339 114 117 3 864.35 2.91 340 114 118 4 839.72 4.32 341 114 153 39 656.22 0.84 342 114 154 40 656.22 0.84 343 115 118 3 849.57 0.60 344 115 119 4 849.56 0.60 345 116 119 3 852.94 0.32 346 116 120 4 839.70 4.32 347 116 121 5 822.11 5.70 348 116 123 7 722.37 30.28 349 117 120 3 852.93 0.32 350 117 121 4 828.93 2.29 351 117 122 5 822.09 5.71 352 117 123 6 777.96 2.42 353 117 154 37 689.08 6.14 354 117 159 42 677.08 0.85 355 117 160 43 673.18 0.26 356 122 159 37 775.80 2.93 357 123 159 36 775.86 2.93 358 123 160 37 775.81 2.94 359 123 161 38 775.81 2.93 360 124 161 37 775.81 2.93 361 124 163 39 775.76 2.92 362 124 207 83 194.19 0.36 363 124 230 106 116.34 0.23 364 125 147 22 776.37 2.92 365 125 150 25 776.29 2.92 366 125 157 32 776.08 2.92 367 125 160 35 775.97 2.94 368 125 161 36 775.88 2.93 369 125 162 37 775.81 2.93 370 125 163 38 775.81 2.93 371 126 163 37 775.81 2.93 372 126 165 39 775.78 2.93 373 126 230 104 117.06 0.26 374 127 143 16 776.48 2.94 375 127 146 19 776.44 2.93 376 127 147 20 776.44 2.93 377 127 162 35 775.99 2.93 378 127 163 36 775.86 2.94 379 127 164 37 775.82 2.94 380 127 165 38 775.81 2.93 381 127 188 61 236.52 3.42 382 128 142 14 776.61 2.89 383 128 143 15 776.51 2.92 384 128 146 18 776.50 2.92 385 128 164 36 775.84 2.94 386 128 165 37 775.82 2.93 387 129 133 4 830.41 0.96 388 129 134 5 830.19 0.90 389 129 139 10 776.61 2.91 390 129 142 13 776.61 2.89 391 129 164 35 776.00 2.93 392 129 167 38 775.77 2.93 393 129 168 39 775.74 2.92 394 130 133 3 837.19 2.44 395 130 134 4 830.41 0.96 396 130 167 37 775.78 2.94 397 130 168 38 775.77 2.94 398 131 134 3 838.78 2.79 399 131 135 4 834.33 1.01 400 131 168 37 775.77 2.93 401 131 172 41 731.73 1.40 402 131 174 43 698.73 0.83 403 132 135 3 856.24 3.12 404 132 136 4 838.00 2.81 405 133 136 3 857.93 4.86 406 133 137 4 830.25 0.89 407 133 138 5 777.56 2.52 408 133 142 9 776.72 2.94 409 134 137 3 837.94 2.85 410 134 139 5 776.76 2.92 411 134 168 34 775.70 2.87 412 138 141 3 844.56 0.43 413 138 142 4 834.15 3.16 414 139 142 3 853.65 4.38 415 139 143 4 826.48 0.68 416 140 144 4 821.35 2.90 417 140 187 47 236.75 3.47 418 141 144 3 868.86 3.22 419 141 145 4 842.83 1.19 420 142 145 3 841.80 0.78 421 142 146 4 830.91 1.16 422 143 146 3 844.08 0.86 423 143 147 4 843.05 1.39 424 143 164 21 775.98 2.92 425 143 188 45 236.58 3.41 426 144 147 3 857.66 5.92 427 144 148 4 843.07 1.38 428 144 191 47 236.56 3.41 429 145 148 3 843.23 1.30 430 145 149 4 843.24 1.29 431 146 149 3 870.76 2.19 432 146 150 4 864.42 5.36 433 147 150 3 875.68 0.41 434 147 151 4 842.70 0.97 435 147 157 10 783.33 5.92 436 147 162 15 776.00 2.93 437 147 188 41 236.62 3.42 438 147 191 44 236.56 3.41 439 147 192 45 236.39 3.47 440 148 151 3 842.70 0.97 441 148 152 4 842.70 0.97 442 148 191 43 236.61 3.42 443 148 195 47 232.94 1.84 444 149 152 3 862.57 6.15 445 149 153 4 842.47 0.14 446 150 153 3 843.93 1.90 447 150 154 4 834.55 2.79 448 150 157 7 785.92 5.17 449 151 154 3 834.41 2.86 450 151 157 6 787.96 3.62 451 151 191 40 236.59 3.41 452 151 192 41 236.52 3.42 453 151 195 44 231.50 1.65 454 151 196 45 225.16 2.43 455 154 157 3 900.57 0.18 456 157 160 3 825.38 1.08 457 157 192 35 235.96 3.52 458 157 196 39 212.58 8.59 459 157 204 47 194.39 0.36 460 160 204 44 194.40 0.35 461 161 205 44 194.28 0.35 462 161 207 46 194.19 0.36 463 162 204 42 194.68 0.33 464 162 205 43 194.46 0.33 465 162 206 44 194.25 0.34 466 162 207 45 194.24 0.34 467 163 207 44 194.24 0.34 468 163 230 67 116.89 0.18 469 164 184 20 236.52 3.38 470 164 185 21 236.39 3.42 471 164 207 43 194.47 0.32 472 164 209 45 194.11 0.34 473 165 209 44 194.09 0.33 474 166 169 3 865.48 2.21 475 166 170 4 857.45 1.84 476 166 176 10 237.33 3.23 477 166 208 42 194.78 0.18 478 166 209 43 194.31 0.33 479 166 210 44 194.12 0.34 480 167 170 3 861.36 0.10 481 167 172 5 842.56 0.02 482 168 172 4 834.51 4.00 483 168 174 6 691.54 0.11 484 168 176 8 370.56 60.68 485 168 180 12 237.69 3.28 486 169 172 3 859.70 3.91 487 169 174 5 493.78 0.83 488 169 175 6 303.61 29.64 489 169 176 7 239.79 2.25 490 170 210 40 194.41 0.26 491 170 211 41 194.36 0.28 492 176 180 4 811.13 3.42 493 176 208 32 195.62 0.51 494 177 180 3 846.76 0.65 495 177 181 4 832.43 4.44 496 178 181 3 844.69 1.69 497 178 182 4 844.96 1.56 498 178 219 41 120.95 0.02 499 178 220 42 120.73 0.05 500 178 223 45 120.39 0.09 501 179 182 3 848.12 0.01 502 179 183 4 844.98 1.56 503 179 223 44 120.42 0.10 504 180 183 3 869.85 4.15 505 180 184 4 848.09 0.01 506 181 184 3 872.13 5.29 507 181 185 4 841.69 0.10 508 181 208 27 195.68 0.55 509 181 220 39 120.73 0.05 510 181 226 45 119.64 0.03 511 182 185 3 871.70 5.51 512 182 186 4 844.73 1.68 513 182 223 41 119.92 0.10 514 182 224 42 119.93 0.10 515 182 225 43 119.71 0.06 516 182 226 44 119.70 0.06 517 183 186 3 871.70 5.51 518 183 187 4 849.07 3.85 519 184 187 3 849.96 3.73 520 184 188 4 844.22 6.28 521 185 188 3 848.98 3.90 522 185 189 4 848.97 3.90 523 185 206 21 196.32 0.85 524 185 207 22 196.32 0.85 525 185 208 23 196.25 0.83 526 185 225 40 119.62 0.03 527 185 226 41 119.56 0.03 528 186 189 3 859.31 1.27 529 186 190 4 848.97 3.90 530 186 225 39 119.64 0.03 531 187 190 3 858.74 0.99 532 187 191 4 834.55 1.21 533 188 191 3 849.97 5.38 534 188 192 4 845.66 4.36 535 188 206 18 196.52 0.82 536 189 192 3 852.47 0.96 537 189 193 4 848.26 1.14 538 189 201 12 197.31 0.92 539 189 202 13 197.27 0.90 540 189 204 15 196.96 0.86 541 189 206 17 196.45 0.79 542 189 225 36 119.59 0.04 543 189 227 38 119.53 0.04 544 190 193 3 849.21 1.03 545 190 194 4 842.07 2.53 546 191 194 3 854.83 1.34 547 191 195 4 848.85 0.85 548 192 195 3 859.11 0.80 549 192 196 4 829.33 3.82 550 192 201 9 197.31 0.92 551 192 204 12 196.96 0.85 552 193 197 4 318.31 28.32 553 193 198 5 197.40 0.96 554 193 201 8 197.33 0.92 555 196 200 4 820.90 0.15 556 197 200 3 858.23 6.08 557 197 201 4 838.47 0.12 558 198 201 3 844.75 1.98 559 198 202 4 838.75 0.27 560 199 202 3 845.12 2.12 561 199 203 4 840.12 0.40 562 200 203 3 861.29 2.85 563 200 204 4 840.11 0.39 564 201 204 3 843.65 0.41 565 204 227 23 118.75 0.21 566 205 227 22 118.62 0.19 567 206 225 19 119.23 0.08 568 206 226 20 119.05 0.17 569 206 227 21 118.77 0.12 570 207 227 20 118.85 0.08 571 207 228 21 118.58 0.12 572 207 229 22 118.20 0.18 573 207 230 23 117.55 0.48 574 208 212 4 838.59 0.14 575 208 220 12 119.41 0.36 576 208 226 18 119.23 0.11 577 208 228 20 118.85 0.08 578 208 229 21 118.49 0.20 579 208 230 22 118.42 0.21 580 209 230 21 118.06 0.34 581 211 216 5 827.77 5.54 582 212 216 4 825.36 6.25 583 212 229 17 119.01 0.06 584 212 231 19 118.84 0.12 585 213 216 3 842.09 2.11 586 213 217 4 755.57 31.53 587 213 234 21 117.12 0.72 588 213 239 26 57.37 0.35 589 213 243 30 49.77 1.60 590 214 239 25 55.08 0.77 591 214 242 28 50.08 1.74 592 214 243 29 50.04 1.74 593 216 219 3 862.63 2.54 594 216 220 4 841.40 1.33 595 217 220 3 875.20 3.10 596 217 229 12 119.71 0.07 597 217 243 26 45.82 0.31 598 217 246 29 41.20 0.04 599 218 221 3 838.74 0.04 600 218 246 28 41.33 0.01 601 220 223 3 864.56 2.23 602 220 226 6 119.98 0.12 603 220 229 9 119.81 0.07 604 220 247 27 39.73 0.78 605 221 246 25 39.73 0.77 606 221 247 26 38.61 0.38 607 223 226 3 846.64 5.79 608 226 229 3 987.50 4.55 609 226 247 21 38.54 0.35 610 229 243 14 42.72 0.61 611 231 234 3 886.47 2.41 612 231 235 4 840.72 1.52 613 231 240 9 176.50 28.03 614 231 243 12 48.59 1.02 615 232 235 3 869.12 3.33 616 233 236 3 878.47 1.34 617 233 237 4 852.66 4.21 618 234 237 3 857.14 1.97 619 234 239 5 590.07 101.49 620 234 240 6 243.54 2.75 621 234 243 9 240.87 4.09 622 235 240 5 243.54 2.75 623 237 240 3 884.60 0.10 624 238 241 3 883.33 0.73 625 239 242 3 868.63 0.22 626 239 243 4 853.54 7.34 627 240 243 3 868.64 0.21 628 240 244 4 851.15 8.54 629 241 244 3 858.29 4.96 630 241 245 4 850.84 8.69 631 242 245 3 854.34 6.94 632 242 246 4 833.60 1.30 633 243 246 3 837.78 3.40 634 245 248 3 826.33 3.07